Egln2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Egln2
Entrez Gene ID	112406
Full Name	egl-9 family hypoxia-inducible factor 2
Synonyms	0610011A13Rik,C85656,Hif-p4h-1,Ier4,Phd1,SM-20
General protein information	Preferred Names egl-9 family hypoxia-inducible factor 2 Names egl nine homolog 2 HIF-PH1 HIF-prolyl hydroxylase 1 HPH-1 falkor hypoxia-inducible factor prolyl hydroxylase 1 immediate early response 4 prolyl hydroxylase domain-containing protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 A3\|7 15.83 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017321929.1	XP_017177418.1	egl nine homolog 2 isoform X1
XM_030241990.1	XP_030097850.1	egl nine homolog 2 isoform X2
NM_053208.4	NP_444438.2	egl nine homolog 2 isoform 1
XM_030241989.1	XP_030097849.1	egl nine homolog 2 isoform X1
XM_030241991.1	XP_030097851.1	egl nine homolog 2 isoform X2
NM_001357767.1	NP_001344696.1	egl nine homolog 2 isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EGLN2	NP_001233442.1
Xenopus tropicalis (tropical clawed frog)	egln2	XP_002938856.2
Homo sapiens (human)	EGLN2	NP_542770.2
Macaca mulatta (Rhesus monkey)	EGLN2	XP_001099832.1
Mus musculus (house mouse)	Egln2	NP_444438.2
Rattus norvegicus (Norway rat)	Egln2	NP_001004083.1
Canis lupus familiaris (dog)	EGLN2	XP_541609.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of Prolyl-Hydroxylase 1 Protects against Biliary Fibrosis via Attenuated Activation of Hepatic Stellate Cells.
Strowitzki MJ, Kirchberg J, Tuffs C, Schiedeck M, Ritter AS, Biller M, Harnoss JM, Lasitschka F, Schmidt T, Radhakrishnan P, Ulrich A, Schneider M
The American journal of pathology188(12)2826-2838(2018 12)

Regulation of hypoxia inducible factor/prolyl hydroxylase binding domain proteins 1 by PPARα and high salt diet.
Ozurumba E, Mathew O, Ranganna K, Choi M, Oyekan A
Journal of basic and clinical physiology and pharmacology29(2)165-173(2018 Mar)

Hypoxia-inducible factor prolyl-4-hydroxylase-1 is a convergent point in the reciprocal negative regulation of NF-κB and p53 signaling pathways.
Ullah K, Rosendahl AH, Izzi V, Bergmann U, Pihlajaniemi T, Mäki JM, Myllyharju J
Scientific reports7(1)17220(2017 12)

Opposing regulation and roles for PHD3 in lung dendritic cells and alveolar macrophages.
Tavernier SJ, Vanlangenakker N, Vetters J, Carmeliet P, Janssens S, Lambrecht BN
Journal of leukocyte biology102(4)1115-1126(2017 10)

Hypoxia-inducible factor 1-alpha does not regulate osteoclastogenesis but enhances bone resorption activity via prolyl-4-hydroxylase 2.
Hulley PA, Bishop T, Vernet A, Schneider JE, Edwards JR, Athanasou NA, Knowles HJ
The Journal of pathology242(3)322-333(2017 07)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Egln2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Egln2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu81894	NM_001357767.1 Latest version!	Mus musculus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu81894	XM_030241991.1 Latest version!	Mus musculus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu81894	XM_017321929.1 Latest version!	Mus musculus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu81894	XM_030241990.1 Latest version!	Mus musculus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu23401	NM_053208.4 Latest version!	Mus musculus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$188.30 $269.00
OMu23401	XM_030241989.1 Latest version!	Mus musculus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Egln2 ( NM_001357767.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344696.1 Mus musculus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu81894
Clone ID Related Accession (Same CDS sequence)	XM_017321929.1 , XM_030241990.1 , XM_030241991.1 , NM_001357767.1
Accession Version	NM_001357767.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570982400000
Organism	Mus musculus(house mouse)
Product	egl nine homolog 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC157553.10. On Nov 4, 2017 this sequence version replaced XM_017321929.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.580881.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACAGCC CGTGCCAGCC GCAGGCCCTG AATCAAGCTC TCCCTCAGTT GCCAGGGTCT 
GTGTCAGAGT CCTTGGAGTC TAGCCGAGCC AGAATGGGGG TGGAGAGTTA CCTGCCCTGC 
CCTCTGCTCC CGGCCTATCA CCGTCCAGGA GCATCTGGGG AGGCCTCGGC TGGCAATGGG 
ACCCCCAGAA CCACAGCCAC TGCTACTACG ACCACTGCCA GTCCCCTGCG GGAGGGCTTT 
GGTGGGCAGG ATGGTGGTGA GCTTTGGCCA CTGCAGAGTG AAGGTGCTGC TGCGTTGGTC 
ACCAAGGAGT GCCAGCGACT GGCGGCCCAG GGTGCCCGGC CTGAGGCCCC CAAACGGAAG 
TGGGCCAAGG ATGGTGGGGA TGCCCCTTCA CCCAGCAAGC GACCGTGGGC CAGGCAAGAG 
AACCAGGAGG CCAAGGGGGA AAGTGGTATG GGCTGTGACA GCGGTGCCAG CAACAGCAGC 
AGCAGCAGCA GCAACACTAC CAGTAGCAGT GGCGAGGCCA TGGTGGCGTG TTATCCAGGC 
AATGGGCTCG GGTACGTGAG GCATGTTGAC AATCCCCACG GCGATGGGCG CTGCATCACC 
TGTATCTATT ACCTGAATCA GAACTGGGAT GTTAAGGTGC ATGGCGGCCT GCTGCAGATC 
TTCCCCGAGG GTCGGCCAGT GGTAGCCAAC ATCGAGCCAC TCTTTGACCG GTTGCTCATT 
TTCTGGTCTG ACCGACGGAA CCCACATGAG GTGAAGCCAG CCTATGCCAC CAGGTACGCC 
ATCACTGTCT GGTATTTTGA TGCCAAGGAA CGGGCAGCAG CCAGAGACAA GTATCAGCTA 
GCATCGGGAC AGAAAGGTGT TCAAGTACCA GTATCACAGC CAACTACACC TACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344696.1
CDS	231..1127
Translation	MDSPCQPQALNQALPQLPGSVSESLESSRARMGVESYLPCPLLPAYHRPGASGEASAGNGTPRTTATATTTTASPLREGFGGQDGGELWPLQSEGAAALVTKECQRLAAQGARPEAPKRKWAKDGGDAPSPSKRPWARQENQEAKGESGMGCDSGASNSSSSSSNTTSSSGEAMVACYPGNGLGYVRHVDNPHGDGRCITCIYYLNQNWDVKVHGGLLQIFPEGRPVVANIEPLFDRLLIFWSDRRNPHEVKPAYATRYAITVWYFDAKERAAARDKYQLASGQKGVQVPVSQPTTPT

Target ORF information:

RefSeq Version	NM_001357767.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357767.1

ORF Insert Sequence:

ATGGACAGCC CGTGCCAGCC GCAGGCCCTG AATCAAGCTC TCCCTCAGTT GCCAGGGTCT 
GTGTCAGAGT CCTTGGAGTC TAGCCGAGCC AGAATGGGGG TGGAGAGTTA CCTGCCCTGC 
CCTCTGCTCC CGGCCTATCA CCGTCCAGGA GCATCTGGGG AGGCCTCGGC TGGCAATGGG 
ACCCCCAGAA CCACAGCCAC TGCTACTACG ACCACTGCCA GTCCCCTGCG GGAGGGCTTT 
GGTGGGCAGG ATGGTGGTGA GCTTTGGCCA CTGCAGAGTG AAGGTGCTGC TGCGTTGGTC 
ACCAAGGAGT GCCAGCGACT GGCGGCCCAG GGTGCCCGGC CTGAGGCCCC CAAACGGAAG 
TGGGCCAAGG ATGGTGGGGA TGCCCCTTCA CCCAGCAAGC GACCGTGGGC CAGGCAAGAG 
AACCAGGAGG CCAAGGGGGA AAGTGGTATG GGCTGTGACA GCGGTGCCAG CAACAGCAGC 
AGCAGCAGCA GCAACACTAC CAGTAGCAGT GGCGAGGCCA TGGTGGCGTG TTATCCAGGC 
AATGGGCTCG GGTACGTGAG GCATGTTGAC AATCCCCACG GCGATGGGCG CTGCATCACC 
TGTATCTATT ACCTGAATCA GAACTGGGAT GTTAAGGTGC ATGGCGGCCT GCTGCAGATC 
TTCCCCGAGG GTCGGCCAGT GGTAGCCAAC ATCGAGCCAC TCTTTGACCG GTTGCTCATT 
TTCTGGTCTG ACCGACGGAA CCCACATGAG GTGAAGCCAG CCTATGCCAC CAGGTACGCC 
ATCACTGTCT GGTATTTTGA TGCCAAGGAA CGGGCAGCAG CCAGAGACAA GTATCAGCTA 
GCATCGGGAC AGAAAGGTGT TCAAGTACCA GTATCACAGC CAACTACACC TACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egln2 ( XM_017321929.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177418.1 Mus musculus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu81894
Clone ID Related Accession (Same CDS sequence)	XM_017321929.1 , XM_030241990.1 , XM_030241991.1 , NM_001357767.1
Accession Version	XM_017321929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(House mouse)
Product	egl nine homolog 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CGTGCCAGCC GCAGGCCCTG AATCAAGCTC TCCCTCAGTT GCCAGGGTCT 
GTGTCAGAGT CCTTGGAGTC TAGCCGAGCC AGAATGGGGG TGGAGAGTTA CCTGCCCTGC 
CCTCTGCTCC CGGCCTATCA CCGTCCAGGA GCATCTGGGG AGGCCTCGGC TGGCAATGGG 
ACCCCCAGAA CCACAGCCAC TGCTACTACG ACCACTGCCA GTCCCCTGCG GGAGGGCTTT 
GGTGGGCAGG ATGGTGGTGA GCTTTGGCCA CTGCAGAGTG AAGGTGCTGC TGCGTTGGTC 
ACCAAGGAGT GCCAGCGACT GGCGGCCCAG GGTGCCCGGC CTGAGGCCCC CAAACGGAAG 
TGGGCCAAGG ATGGTGGGGA TGCCCCTTCA CCCAGCAAGC GACCGTGGGC CAGGCAAGAG 
AACCAGGAGG CCAAGGGGGA AAGTGGTATG GGCTGTGACA GCGGTGCCAG CAACAGCAGC 
AGCAGCAGCA GCAACACTAC CAGTAGCAGT GGCGAGGCCA TGGTGGCGTG TTATCCAGGC 
AATGGGCTCG GGTACGTGAG GCATGTTGAC AATCCCCACG GCGATGGGCG CTGCATCACC 
TGTATCTATT ACCTGAATCA GAACTGGGAT GTTAAGGTGC ATGGCGGCCT GCTGCAGATC 
TTCCCCGAGG GTCGGCCAGT GGTAGCCAAC ATCGAGCCAC TCTTTGACCG GTTGCTCATT 
TTCTGGTCTG ACCGACGGAA CCCACATGAG GTGAAGCCAG CCTATGCCAC CAGGTACGCC 
ATCACTGTCT GGTATTTTGA TGCCAAGGAA CGGGCAGCAG CCAGAGACAA GTATCAGCTA 
GCATCGGGAC AGAAAGGTGT TCAAGTACCA GTATCACAGC CAACTACACC TACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177418.1
CDS	113..1009
Translation	MDSPCQPQALNQALPQLPGSVSESLESSRARMGVESYLPCPLLPAYHRPGASGEASAGNGTPRTTATATTTTASPLREGFGGQDGGELWPLQSEGAAALVTKECQRLAAQGARPEAPKRKWAKDGGDAPSPSKRPWARQENQEAKGESGMGCDSGASNSSSSSSNTTSSSGEAMVACYPGNGLGYVRHVDNPHGDGRCITCIYYLNQNWDVKVHGGLLQIFPEGRPVVANIEPLFDRLLIFWSDRRNPHEVKPAYATRYAITVWYFDAKERAAARDKYQLASGQKGVQVPVSQPTTPT

Target ORF information:

RefSeq Version	XM_017321929.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321929.1

ORF Insert Sequence:

ATGGACAGCC CGTGCCAGCC GCAGGCCCTG AATCAAGCTC TCCCTCAGTT GCCAGGGTCT 
GTGTCAGAGT CCTTGGAGTC TAGCCGAGCC AGAATGGGGG TGGAGAGTTA CCTGCCCTGC 
CCTCTGCTCC CGGCCTATCA CCGTCCAGGA GCATCTGGGG AGGCCTCGGC TGGCAATGGG 
ACCCCCAGAA CCACAGCCAC TGCTACTACG ACCACTGCCA GTCCCCTGCG GGAGGGCTTT 
GGTGGGCAGG ATGGTGGTGA GCTTTGGCCA CTGCAGAGTG AAGGTGCTGC TGCGTTGGTC 
ACCAAGGAGT GCCAGCGACT GGCGGCCCAG GGTGCCCGGC CTGAGGCCCC CAAACGGAAG 
TGGGCCAAGG ATGGTGGGGA TGCCCCTTCA CCCAGCAAGC GACCGTGGGC CAGGCAAGAG 
AACCAGGAGG CCAAGGGGGA AAGTGGTATG GGCTGTGACA GCGGTGCCAG CAACAGCAGC 
AGCAGCAGCA GCAACACTAC CAGTAGCAGT GGCGAGGCCA TGGTGGCGTG TTATCCAGGC 
AATGGGCTCG GGTACGTGAG GCATGTTGAC AATCCCCACG GCGATGGGCG CTGCATCACC 
TGTATCTATT ACCTGAATCA GAACTGGGAT GTTAAGGTGC ATGGCGGCCT GCTGCAGATC 
TTCCCCGAGG GTCGGCCAGT GGTAGCCAAC ATCGAGCCAC TCTTTGACCG GTTGCTCATT 
TTCTGGTCTG ACCGACGGAA CCCACATGAG GTGAAGCCAG CCTATGCCAC CAGGTACGCC 
ATCACTGTCT GGTATTTTGA TGCCAAGGAA CGGGCAGCAG CCAGAGACAA GTATCAGCTA 
GCATCGGGAC AGAAAGGTGT TCAAGTACCA GTATCACAGC CAACTACACC TACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egln2 ( XM_030241990.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030097850.1 Mus musculus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu81894
Clone ID Related Accession (Same CDS sequence)	XM_017321929.1 , XM_030241990.1 , XM_030241991.1 , NM_001357767.1
Accession Version	XM_030241990.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	egl nine homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CGTGCCAGCC GCAGGCCCTG AATCAAGCTC TCCCTCAGTT GCCAGGGTCT 
GTGTCAGAGT CCTTGGAGTC TAGCCGAGCC AGAATGGGGG TGGAGAGTTA CCTGCCCTGC 
CCTCTGCTCC CGGCCTATCA CCGTCCAGGA GCATCTGGGG AGGCCTCGGC TGGCAATGGG 
ACCCCCAGAA CCACAGCCAC TGCTACTACG ACCACTGCCA GTCCCCTGCG GGAGGGCTTT 
GGTGGGCAGG ATGGTGGTGA GCTTTGGCCA CTGCAGAGTG AAGGTGCTGC TGCGTTGGTC 
ACCAAGGAGT GCCAGCGACT GGCGGCCCAG GGTGCCCGGC CTGAGGCCCC CAAACGGAAG 
TGGGCCAAGG ATGGTGGGGA TGCCCCTTCA CCCAGCAAGC GACCGTGGGC CAGGCAAGAG 
AACCAGGAGG CCAAGGGGGA AAGTGGTATG GGCTGTGACA GCGGTGCCAG CAACAGCAGC 
AGCAGCAGCA GCAACACTAC CAGTAGCAGT GGCGAGGCCA TGGTGGCGTG TTATCCAGGC 
AATGGGCTCG GGTACGTGAG GCATGTTGAC AATCCCCACG GCGATGGGCG CTGCATCACC 
TGTATCTATT ACCTGAATCA GAACTGGGAT GTTAAGGTGC ATGGCGGCCT GCTGCAGATC 
TTCCCCGAGG GTCGGCCAGT GGTAGCCAAC ATCGAGCCAC TCTTTGACCG GTTGCTCATT 
TTCTGGTCTG ACCGACGGAA CCCACATGAG GTGAAGCCAG CCTATGCCAC CAGGTACGCC 
ATCACTGTCT GGTATTTTGA TGCCAAGGAA CGGGCAGCAG CCAGAGACAA GTATCAGCTA 
GCATCGGGAC AGAAAGGTGT TCAAGTACCA GTATCACAGC CAACTACACC TACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030097850.1
CDS	345..1241
Translation	MDSPCQPQALNQALPQLPGSVSESLESSRARMGVESYLPCPLLPAYHRPGASGEASAGNGTPRTTATATTTTASPLREGFGGQDGGELWPLQSEGAAALVTKECQRLAAQGARPEAPKRKWAKDGGDAPSPSKRPWARQENQEAKGESGMGCDSGASNSSSSSSNTTSSSGEAMVACYPGNGLGYVRHVDNPHGDGRCITCIYYLNQNWDVKVHGGLLQIFPEGRPVVANIEPLFDRLLIFWSDRRNPHEVKPAYATRYAITVWYFDAKERAAARDKYQLASGQKGVQVPVSQPTTPT

Target ORF information:

RefSeq Version	XM_030241990.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030241990.1

ORF Insert Sequence:

ATGGACAGCC CGTGCCAGCC GCAGGCCCTG AATCAAGCTC TCCCTCAGTT GCCAGGGTCT 
GTGTCAGAGT CCTTGGAGTC TAGCCGAGCC AGAATGGGGG TGGAGAGTTA CCTGCCCTGC 
CCTCTGCTCC CGGCCTATCA CCGTCCAGGA GCATCTGGGG AGGCCTCGGC TGGCAATGGG 
ACCCCCAGAA CCACAGCCAC TGCTACTACG ACCACTGCCA GTCCCCTGCG GGAGGGCTTT 
GGTGGGCAGG ATGGTGGTGA GCTTTGGCCA CTGCAGAGTG AAGGTGCTGC TGCGTTGGTC 
ACCAAGGAGT GCCAGCGACT GGCGGCCCAG GGTGCCCGGC CTGAGGCCCC CAAACGGAAG 
TGGGCCAAGG ATGGTGGGGA TGCCCCTTCA CCCAGCAAGC GACCGTGGGC CAGGCAAGAG 
AACCAGGAGG CCAAGGGGGA AAGTGGTATG GGCTGTGACA GCGGTGCCAG CAACAGCAGC 
AGCAGCAGCA GCAACACTAC CAGTAGCAGT GGCGAGGCCA TGGTGGCGTG TTATCCAGGC 
AATGGGCTCG GGTACGTGAG GCATGTTGAC AATCCCCACG GCGATGGGCG CTGCATCACC 
TGTATCTATT ACCTGAATCA GAACTGGGAT GTTAAGGTGC ATGGCGGCCT GCTGCAGATC 
TTCCCCGAGG GTCGGCCAGT GGTAGCCAAC ATCGAGCCAC TCTTTGACCG GTTGCTCATT 
TTCTGGTCTG ACCGACGGAA CCCACATGAG GTGAAGCCAG CCTATGCCAC CAGGTACGCC 
ATCACTGTCT GGTATTTTGA TGCCAAGGAA CGGGCAGCAG CCAGAGACAA GTATCAGCTA 
GCATCGGGAC AGAAAGGTGT TCAAGTACCA GTATCACAGC CAACTACACC TACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egln2 ( XM_030241991.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030097851.1 Mus musculus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu81894
Clone ID Related Accession (Same CDS sequence)	XM_017321929.1 , XM_030241990.1 , XM_030241991.1 , NM_001357767.1
Accession Version	XM_030241991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	egl nine homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CGTGCCAGCC GCAGGCCCTG AATCAAGCTC TCCCTCAGTT GCCAGGGTCT 
GTGTCAGAGT CCTTGGAGTC TAGCCGAGCC AGAATGGGGG TGGAGAGTTA CCTGCCCTGC 
CCTCTGCTCC CGGCCTATCA CCGTCCAGGA GCATCTGGGG AGGCCTCGGC TGGCAATGGG 
ACCCCCAGAA CCACAGCCAC TGCTACTACG ACCACTGCCA GTCCCCTGCG GGAGGGCTTT 
GGTGGGCAGG ATGGTGGTGA GCTTTGGCCA CTGCAGAGTG AAGGTGCTGC TGCGTTGGTC 
ACCAAGGAGT GCCAGCGACT GGCGGCCCAG GGTGCCCGGC CTGAGGCCCC CAAACGGAAG 
TGGGCCAAGG ATGGTGGGGA TGCCCCTTCA CCCAGCAAGC GACCGTGGGC CAGGCAAGAG 
AACCAGGAGG CCAAGGGGGA AAGTGGTATG GGCTGTGACA GCGGTGCCAG CAACAGCAGC 
AGCAGCAGCA GCAACACTAC CAGTAGCAGT GGCGAGGCCA TGGTGGCGTG TTATCCAGGC 
AATGGGCTCG GGTACGTGAG GCATGTTGAC AATCCCCACG GCGATGGGCG CTGCATCACC 
TGTATCTATT ACCTGAATCA GAACTGGGAT GTTAAGGTGC ATGGCGGCCT GCTGCAGATC 
TTCCCCGAGG GTCGGCCAGT GGTAGCCAAC ATCGAGCCAC TCTTTGACCG GTTGCTCATT 
TTCTGGTCTG ACCGACGGAA CCCACATGAG GTGAAGCCAG CCTATGCCAC CAGGTACGCC 
ATCACTGTCT GGTATTTTGA TGCCAAGGAA CGGGCAGCAG CCAGAGACAA GTATCAGCTA 
GCATCGGGAC AGAAAGGTGT TCAAGTACCA GTATCACAGC CAACTACACC TACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030097851.1
CDS	461..1357
Translation	MDSPCQPQALNQALPQLPGSVSESLESSRARMGVESYLPCPLLPAYHRPGASGEASAGNGTPRTTATATTTTASPLREGFGGQDGGELWPLQSEGAAALVTKECQRLAAQGARPEAPKRKWAKDGGDAPSPSKRPWARQENQEAKGESGMGCDSGASNSSSSSSNTTSSSGEAMVACYPGNGLGYVRHVDNPHGDGRCITCIYYLNQNWDVKVHGGLLQIFPEGRPVVANIEPLFDRLLIFWSDRRNPHEVKPAYATRYAITVWYFDAKERAAARDKYQLASGQKGVQVPVSQPTTPT

Target ORF information:

RefSeq Version	XM_030241991.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030241991.1

ORF Insert Sequence:

ATGGACAGCC CGTGCCAGCC GCAGGCCCTG AATCAAGCTC TCCCTCAGTT GCCAGGGTCT 
GTGTCAGAGT CCTTGGAGTC TAGCCGAGCC AGAATGGGGG TGGAGAGTTA CCTGCCCTGC 
CCTCTGCTCC CGGCCTATCA CCGTCCAGGA GCATCTGGGG AGGCCTCGGC TGGCAATGGG 
ACCCCCAGAA CCACAGCCAC TGCTACTACG ACCACTGCCA GTCCCCTGCG GGAGGGCTTT 
GGTGGGCAGG ATGGTGGTGA GCTTTGGCCA CTGCAGAGTG AAGGTGCTGC TGCGTTGGTC 
ACCAAGGAGT GCCAGCGACT GGCGGCCCAG GGTGCCCGGC CTGAGGCCCC CAAACGGAAG 
TGGGCCAAGG ATGGTGGGGA TGCCCCTTCA CCCAGCAAGC GACCGTGGGC CAGGCAAGAG 
AACCAGGAGG CCAAGGGGGA AAGTGGTATG GGCTGTGACA GCGGTGCCAG CAACAGCAGC 
AGCAGCAGCA GCAACACTAC CAGTAGCAGT GGCGAGGCCA TGGTGGCGTG TTATCCAGGC 
AATGGGCTCG GGTACGTGAG GCATGTTGAC AATCCCCACG GCGATGGGCG CTGCATCACC 
TGTATCTATT ACCTGAATCA GAACTGGGAT GTTAAGGTGC ATGGCGGCCT GCTGCAGATC 
TTCCCCGAGG GTCGGCCAGT GGTAGCCAAC ATCGAGCCAC TCTTTGACCG GTTGCTCATT 
TTCTGGTCTG ACCGACGGAA CCCACATGAG GTGAAGCCAG CCTATGCCAC CAGGTACGCC 
ATCACTGTCT GGTATTTTGA TGCCAAGGAA CGGGCAGCAG CCAGAGACAA GTATCAGCTA 
GCATCGGGAC AGAAAGGTGT TCAAGTACCA GTATCACAGC CAACTACACC TACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egln2 ( NM_053208.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_444438.2 Mus musculus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant 1, mRNA.

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CloneID	OMu23401
Clone ID Related Accession (Same CDS sequence)	NM_053208.4 , XM_030241989.1
Accession Version	NM_053208.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	egl nine homolog 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB248412.1, BC086764.1 and BE993075.1. On Apr 7, 2010 this sequence version replaced NM_053208.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ310547.1, AK152056.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACAGCC CGTGCCAGCC GCAGGCCCTG AATCAAGCTC TCCCTCAGTT GCCAGGGTCT 
GTGTCAGAGT CCTTGGAGTC TAGCCGAGCC AGAATGGGGG TGGAGAGTTA CCTGCCCTGC 
CCTCTGCTCC CGGCCTATCA CCGTCCAGGA GCATCTGGGG AGGCCTCGGC TGGCAATGGG 
ACCCCCAGAA CCACAGCCAC TGCTACTACG ACCACTGCCA GTCCCCTGCG GGAGGGCTTT 
GGTGGGCAGG ATGGTGGTGA GCTTTGGCCA CTGCAGAGTG AAGGTGCTGC TGCGTTGGTC 
ACCAAGGAGT GCCAGCGACT GGCGGCCCAG GGTGCCCGGC CTGAGGCCCC CAAACGGAAG 
TGGGCCAAGG ATGGTGGGGA TGCCCCTTCA CCCAGCAAGC GACCGTGGGC CAGGCAAGAG 
AACCAGGAGG CCAAGGGGGA AAGTGGTATG GGCTGTGACA GCGGTGCCAG CAACAGCAGC 
AGCAGCAGCA GCAACACTAC CAGTAGCAGT GGCGAGGCAA GTGCTAGGCT GAGGGAGGAA 
GTCCAGCCCT CTGCACCTGA GCGCCTGGCC CTGGACTATA TTGTGCCTTG CATGCGGTAC 
TATGGTATCT GTGTCAAGGA CAACTTCTTG GGGGCAGTAC TGGGTGGCCG TGTGCTGGCT 
GAGGTGGAAG CCCTGAAGTG GGGCGGGCGT CTTCGTGATG GGCAACTAGT GAGCCAGCGG 
GCGATCCCAC CGCGCAGCAT TCGTGGGGAC CAGATTGCCT GGGTAGAAGG TCACGAGCCA 
GGCTGCCGGA GCATTGGTGC CCTCATGGCT CACGTGGACG CAGTAATCCG CCACTGTGCA 
GGGCGGCTGG GCAACTACGT CATCAATGGG CGCACCAAGG CCATGGTGGC GTGTTATCCA 
GGCAATGGGC TCGGGTACGT GAGGCATGTT GACAATCCCC ACGGCGATGG GCGCTGCATC 
ACCTGTATCT ATTACCTGAA TCAGAACTGG GATGTTAAGG TGCATGGCGG CCTGCTGCAG 
ATCTTCCCCG AGGGTCGGCC AGTGGTAGCC AACATCGAGC CACTCTTTGA CCGGTTGCTC 
ATTTTCTGGT CTGACCGACG GAACCCACAT GAGGTGAAGC CAGCCTATGC CACCAGGTAC 
GCCATCACTG TCTGGTATTT TGATGCCAAG GAACGGGCAG CAGCCAGAGA CAAGTATCAG 
CTAGCATCGG GACAGAAAGG TGTTCAAGTA CCAGTATCAC AGCCAACTAC ACCTACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_444438.2
CDS	350..1609
Translation	MDSPCQPQALNQALPQLPGSVSESLESSRARMGVESYLPCPLLPAYHRPGASGEASAGNGTPRTTATATTTTASPLREGFGGQDGGELWPLQSEGAAALVTKECQRLAAQGARPEAPKRKWAKDGGDAPSPSKRPWARQENQEAKGESGMGCDSGASNSSSSSSNTTSSSGEASARLREEVQPSAPERLALDYIVPCMRYYGICVKDNFLGAVLGGRVLAEVEALKWGGRLRDGQLVSQRAIPPRSIRGDQIAWVEGHEPGCRSIGALMAHVDAVIRHCAGRLGNYVINGRTKAMVACYPGNGLGYVRHVDNPHGDGRCITCIYYLNQNWDVKVHGGLLQIFPEGRPVVANIEPLFDRLLIFWSDRRNPHEVKPAYATRYAITVWYFDAKERAAARDKYQLASGQKGVQVPVSQPTTPT

Target ORF information:

RefSeq Version	NM_053208.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053208.4

ORF Insert Sequence:

ATGGACAGCC CGTGCCAGCC GCAGGCCCTG AATCAAGCTC TCCCTCAGTT GCCAGGGTCT 
GTGTCAGAGT CCTTGGAGTC TAGCCGAGCC AGAATGGGGG TGGAGAGTTA CCTGCCCTGC 
CCTCTGCTCC CGGCCTATCA CCGTCCAGGA GCATCTGGGG AGGCCTCGGC TGGCAATGGG 
ACCCCCAGAA CCACAGCCAC TGCTACTACG ACCACTGCCA GTCCCCTGCG GGAGGGCTTT 
GGTGGGCAGG ATGGTGGTGA GCTTTGGCCA CTGCAGAGTG AAGGTGCTGC TGCGTTGGTC 
ACCAAGGAGT GCCAGCGACT GGCGGCCCAG GGTGCCCGGC CTGAGGCCCC CAAACGGAAG 
TGGGCCAAGG ATGGTGGGGA TGCCCCTTCA CCCAGCAAGC GACCGTGGGC CAGGCAAGAG 
AACCAGGAGG CCAAGGGGGA AAGTGGTATG GGCTGTGACA GCGGTGCCAG CAACAGCAGC 
AGCAGCAGCA GCAACACTAC CAGTAGCAGT GGCGAGGCAA GTGCTAGGCT GAGGGAGGAA 
GTCCAGCCCT CTGCACCTGA GCGCCTGGCC CTGGACTATA TTGTGCCTTG CATGCGGTAC 
TATGGTATCT GTGTCAAGGA CAACTTCTTG GGGGCAGTAC TGGGTGGCCG TGTGCTGGCT 
GAGGTGGAAG CCCTGAAGTG GGGCGGGCGT CTTCGTGATG GGCAACTAGT GAGCCAGCGG 
GCGATCCCAC CGCGCAGCAT TCGTGGGGAC CAGATTGCCT GGGTAGAAGG TCACGAGCCA 
GGCTGCCGGA GCATTGGTGC CCTCATGGCT CACGTGGACG CAGTAATCCG CCACTGTGCA 
GGGCGGCTGG GCAACTACGT CATCAATGGG CGCACCAAGG CCATGGTGGC GTGTTATCCA 
GGCAATGGGC TCGGGTACGT GAGGCATGTT GACAATCCCC ACGGCGATGG GCGCTGCATC 
ACCTGTATCT ATTACCTGAA TCAGAACTGG GATGTTAAGG TGCATGGCGG CCTGCTGCAG 
ATCTTCCCCG AGGGTCGGCC AGTGGTAGCC AACATCGAGC CACTCTTTGA CCGGTTGCTC 
ATTTTCTGGT CTGACCGACG GAACCCACAT GAGGTGAAGC CAGCCTATGC CACCAGGTAC 
GCCATCACTG TCTGGTATTT TGATGCCAAG GAACGGGCAG CAGCCAGAGA CAAGTATCAG 
CTAGCATCGG GACAGAAAGG TGTTCAAGTA CCAGTATCAC AGCCAACTAC ACCTACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egln2 ( XM_030241989.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030097849.1 Mus musculus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu23401
Clone ID Related Accession (Same CDS sequence)	NM_053208.4 , XM_030241989.1
Accession Version	XM_030241989.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	egl nine homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CGTGCCAGCC GCAGGCCCTG AATCAAGCTC TCCCTCAGTT GCCAGGGTCT 
GTGTCAGAGT CCTTGGAGTC TAGCCGAGCC AGAATGGGGG TGGAGAGTTA CCTGCCCTGC 
CCTCTGCTCC CGGCCTATCA CCGTCCAGGA GCATCTGGGG AGGCCTCGGC TGGCAATGGG 
ACCCCCAGAA CCACAGCCAC TGCTACTACG ACCACTGCCA GTCCCCTGCG GGAGGGCTTT 
GGTGGGCAGG ATGGTGGTGA GCTTTGGCCA CTGCAGAGTG AAGGTGCTGC TGCGTTGGTC 
ACCAAGGAGT GCCAGCGACT GGCGGCCCAG GGTGCCCGGC CTGAGGCCCC CAAACGGAAG 
TGGGCCAAGG ATGGTGGGGA TGCCCCTTCA CCCAGCAAGC GACCGTGGGC CAGGCAAGAG 
AACCAGGAGG CCAAGGGGGA AAGTGGTATG GGCTGTGACA GCGGTGCCAG CAACAGCAGC 
AGCAGCAGCA GCAACACTAC CAGTAGCAGT GGCGAGGCAA GTGCTAGGCT GAGGGAGGAA 
GTCCAGCCCT CTGCACCTGA GCGCCTGGCC CTGGACTATA TTGTGCCTTG CATGCGGTAC 
TATGGTATCT GTGTCAAGGA CAACTTCTTG GGGGCAGTAC TGGGTGGCCG TGTGCTGGCT 
GAGGTGGAAG CCCTGAAGTG GGGCGGGCGT CTTCGTGATG GGCAACTAGT GAGCCAGCGG 
GCGATCCCAC CGCGCAGCAT TCGTGGGGAC CAGATTGCCT GGGTAGAAGG TCACGAGCCA 
GGCTGCCGGA GCATTGGTGC CCTCATGGCT CACGTGGACG CAGTAATCCG CCACTGTGCA 
GGGCGGCTGG GCAACTACGT CATCAATGGG CGCACCAAGG CCATGGTGGC GTGTTATCCA 
GGCAATGGGC TCGGGTACGT GAGGCATGTT GACAATCCCC ACGGCGATGG GCGCTGCATC 
ACCTGTATCT ATTACCTGAA TCAGAACTGG GATGTTAAGG TGCATGGCGG CCTGCTGCAG 
ATCTTCCCCG AGGGTCGGCC AGTGGTAGCC AACATCGAGC CACTCTTTGA CCGGTTGCTC 
ATTTTCTGGT CTGACCGACG GAACCCACAT GAGGTGAAGC CAGCCTATGC CACCAGGTAC 
GCCATCACTG TCTGGTATTT TGATGCCAAG GAACGGGCAG CAGCCAGAGA CAAGTATCAG 
CTAGCATCGG GACAGAAAGG TGTTCAAGTA CCAGTATCAC AGCCAACTAC ACCTACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030097849.1
CDS	461..1720
Translation	MDSPCQPQALNQALPQLPGSVSESLESSRARMGVESYLPCPLLPAYHRPGASGEASAGNGTPRTTATATTTTASPLREGFGGQDGGELWPLQSEGAAALVTKECQRLAAQGARPEAPKRKWAKDGGDAPSPSKRPWARQENQEAKGESGMGCDSGASNSSSSSSNTTSSSGEASARLREEVQPSAPERLALDYIVPCMRYYGICVKDNFLGAVLGGRVLAEVEALKWGGRLRDGQLVSQRAIPPRSIRGDQIAWVEGHEPGCRSIGALMAHVDAVIRHCAGRLGNYVINGRTKAMVACYPGNGLGYVRHVDNPHGDGRCITCIYYLNQNWDVKVHGGLLQIFPEGRPVVANIEPLFDRLLIFWSDRRNPHEVKPAYATRYAITVWYFDAKERAAARDKYQLASGQKGVQVPVSQPTTPT

Target ORF information:

RefSeq Version	XM_030241989.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030241989.1

ORF Insert Sequence:

ATGGACAGCC CGTGCCAGCC GCAGGCCCTG AATCAAGCTC TCCCTCAGTT GCCAGGGTCT 
GTGTCAGAGT CCTTGGAGTC TAGCCGAGCC AGAATGGGGG TGGAGAGTTA CCTGCCCTGC 
CCTCTGCTCC CGGCCTATCA CCGTCCAGGA GCATCTGGGG AGGCCTCGGC TGGCAATGGG 
ACCCCCAGAA CCACAGCCAC TGCTACTACG ACCACTGCCA GTCCCCTGCG GGAGGGCTTT 
GGTGGGCAGG ATGGTGGTGA GCTTTGGCCA CTGCAGAGTG AAGGTGCTGC TGCGTTGGTC 
ACCAAGGAGT GCCAGCGACT GGCGGCCCAG GGTGCCCGGC CTGAGGCCCC CAAACGGAAG 
TGGGCCAAGG ATGGTGGGGA TGCCCCTTCA CCCAGCAAGC GACCGTGGGC CAGGCAAGAG 
AACCAGGAGG CCAAGGGGGA AAGTGGTATG GGCTGTGACA GCGGTGCCAG CAACAGCAGC 
AGCAGCAGCA GCAACACTAC CAGTAGCAGT GGCGAGGCAA GTGCTAGGCT GAGGGAGGAA 
GTCCAGCCCT CTGCACCTGA GCGCCTGGCC CTGGACTATA TTGTGCCTTG CATGCGGTAC 
TATGGTATCT GTGTCAAGGA CAACTTCTTG GGGGCAGTAC TGGGTGGCCG TGTGCTGGCT 
GAGGTGGAAG CCCTGAAGTG GGGCGGGCGT CTTCGTGATG GGCAACTAGT GAGCCAGCGG 
GCGATCCCAC CGCGCAGCAT TCGTGGGGAC CAGATTGCCT GGGTAGAAGG TCACGAGCCA 
GGCTGCCGGA GCATTGGTGC CCTCATGGCT CACGTGGACG CAGTAATCCG CCACTGTGCA 
GGGCGGCTGG GCAACTACGT CATCAATGGG CGCACCAAGG CCATGGTGGC GTGTTATCCA 
GGCAATGGGC TCGGGTACGT GAGGCATGTT GACAATCCCC ACGGCGATGG GCGCTGCATC 
ACCTGTATCT ATTACCTGAA TCAGAACTGG GATGTTAAGG TGCATGGCGG CCTGCTGCAG 
ATCTTCCCCG AGGGTCGGCC AGTGGTAGCC AACATCGAGC CACTCTTTGA CCGGTTGCTC 
ATTTTCTGGT CTGACCGACG GAACCCACAT GAGGTGAAGC CAGCCTATGC CACCAGGTAC 
GCCATCACTG TCTGGTATTT TGATGCCAAG GAACGGGCAG CAGCCAGAGA CAAGTATCAG 
CTAGCATCGG GACAGAAAGG TGTTCAAGTA CCAGTATCAC AGCCAACTAC ACCTACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of Prolyl-Hydroxylase 1 Protects against Biliary Fibrosis via Attenuated Activation of Hepatic Stellate Cells.
The American journal of pathology188(12)2826-2838(2018 12)
Strowitzki MJ,Kirchberg J,Tuffs C,Schiedeck M,Ritter AS,Biller M,Harnoss JM,Lasitschka F,Schmidt T,Radhakrishnan P,Ulrich A,Schneider M

Regulation of hypoxia inducible factor/prolyl hydroxylase binding domain proteins 1 by PPARα and high salt diet.
Journal of basic and clinical physiology and pharmacology29(2)165-173(2018 Mar)
Ozurumba E,Mathew O,Ranganna K,Choi M,Oyekan A

Hypoxia-inducible factor prolyl-4-hydroxylase-1 is a convergent point in the reciprocal negative regulation of NF-κB and p53 signaling pathways.
Scientific reports7(1)17220(2017 12)
Ullah K,Rosendahl AH,Izzi V,Bergmann U,Pihlajaniemi T,Mäki JM,Myllyharju J

Opposing regulation and roles for PHD3 in lung dendritic cells and alveolar macrophages.
Journal of leukocyte biology102(4)1115-1126(2017 10)
Tavernier SJ,Vanlangenakker N,Vetters J,Carmeliet P,Janssens S,Lambrecht BN

Hypoxia-inducible factor 1-alpha does not regulate osteoclastogenesis but enhances bone resorption activity via prolyl-4-hydroxylase 2.
The Journal of pathology242(3)322-333(2017 07)
Hulley PA,Bishop T,Vernet A,Schneider JE,Edwards JR,Athanasou NA,Knowles HJ

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Egln2 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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