Rlf cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rlf
Entrez Gene ID	109263
Full Name	rearranged L-myc fusion sequence
Synonyms	9230110M18Rik,AI195322,MommeD8
General protein information	Preferred Names rearranged L-myc fusion sequence Names zinc finger protein Rlf
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D2.2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006502657.4	XP_006502720.1	zinc finger protein Rlf isoform X1
XM_017319915.1	XP_017175404.1	zinc finger protein Rlf isoform X2
XM_017319915.2	XP_017175404.1	zinc finger protein Rlf isoform X1
XM_006502657.3	XP_006502720.1	zinc finger protein Rlf isoform X2
XM_006502658.1	XP_006502721.1	zinc finger protein Rlf isoform X2
NM_001346660.1	NP_001333589.1	zinc finger protein Rlf
XM_006502658.2	XP_006502721.1	zinc finger protein Rlf isoform X1
XM_030253080.1	XP_030108940.1	zinc finger protein Rlf isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RLF	NP_036553.2
Pan troglodytes (chimpanzee)	RLF	XP_524678.1
Rattus norvegicus (Norway rat)	Rlf	XP_233485.6
Danio rerio (zebrafish)	rlf	XP_690924.6
Macaca mulatta (Rhesus monkey)	LOC693503	XP_002808256.1
Bos taurus (cattle)	RLF	NP_001192868.1
Xenopus tropicalis (tropical clawed frog)	rlf	NP_001123386.2
Canis lupus familiaris (dog)	RLF	XP_848535.1
Mus musculus (house mouse)	Rlf	NP_001074482.1
Gallus gallus (chicken)	RLF	XP_417721.4

Gene Ontology
Bibliography

Related articles in PubMed

Loss of Rearranged L-Myc Fusion (RLF) results in defects in heart development in the mouse.
Bourke LM, Del Monte-Nieto G, Outhwaite JE, Bharti V, Pollock PM, Simmons DG, Adam A, Hur SS, Maghzal GJ, Whitelaw E, Stocker R, Suter CM, Harvey RP, Harten SK
Differentiation; research in biological diversity948-20(2017 Mar - Apr)

Control of cardiac jelly dynamics by NOTCH1 and NRG1 defines the building plan for trabeculation.
Del Monte-Nieto G, Ramialison M, Adam AAS, Wu B, Aharonov A, D'Uva G, Bourke LM, Pitulescu ME, Chen H, de la Pompa JL, Shou W, Adams RH, Harten SK, Tzahor E, Zhou B, Harvey RP
Nature557(7705)439-445(2018 05)

The recently identified modifier of murine metastable epialleles, Rearranged L-Myc Fusion, is involved in maintaining epigenetic marks at CpG island shores and enhancers.
Harten SK, Oey H, Bourke LM, Bharti V, Isbel L, Daxinger L, Faou P, Robertson N, Matthews JM, Whitelaw E
BMC biology1321(2015 Mar)

An ENU mutagenesis screen identifies novel and known genes involved in epigenetic processes in the mouse.
Daxinger L, Harten SK, Oey H, Epp T, Isbel L, Huang E, Whitelaw N, Apedaile A, Sorolla A, Yong J, Bharti V, Sutton J, Ashe A, Pang Z, Wallace N, Gerhardt DJ, Blewitt ME, Jeddeloh JA, Whitelaw E
Genome biology14(9)R96(2013)

A genome-wide screen for modifiers of transgene variegation identifies genes with critical roles in development.
Ashe A, Morgan DK, Whitelaw NC, Bruxner TJ, Vickaryous NK, Cox LL, Butterfield NC, Wicking C, Blewitt ME, Wilkins SJ, Anderson GJ, Cox TC, Whitelaw E
Genome biology9(12)R182(2008)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rlf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rlf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu33280	NM_001346660.1 Latest version!	Mus musculus rearranged L-myc fusion sequence (Rlf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu33281	XM_006502658.2 Latest version!	Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu33281	XM_030253080.1 Latest version!	Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu33281	XM_006502657.4 Latest version!	Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu33281	XM_017319915.2 Latest version!	Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OMu33281	XM_017319915.1	Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu33281	XM_006502657.3	Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu33281	XM_006502658.1	Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rlf ( NM_001346660.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001333589.1 Mus musculus rearranged L-myc fusion sequence (Rlf), mRNA.

Price & Availability↑

CloneID	OMu33280
Clone ID Related Accession (Same CDS sequence)	NM_001346660.1
Accession Version	NM_001346660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1530892800000
Organism	Mus musculus(house mouse)
Product	zinc finger protein Rlf
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL606927.12. On Oct 25, 2016 this sequence version replaced XM_006502656.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.107745.1, SRR1660813.25145.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG GAAAGGGAGA CGCCACGGCC GCCGCCGGGG CCGGGGCTGG GGCTGAGGCT 
CCGGCGGTGG CGGGAGCCGA AGTCGAGACT GAGTCCATGG CTCGGGGTCA TCGCCCTGCA 
TCTCCGGCGT CGGGAGCGCC GGGCCTACGA CCGTGTCTCT GGCAGCTGGA GACCGAGCTG 
AGGGAGCAGG AGGTGTCCGA GGTCTCATCT TTGAGCTACT GCCGGAGCTT CTGCCAGACC 
TTATTGCAAT ATGCAAGCAA CAAGAATGCA TCAGAACACA TTGTGTATCT TCTGGAGGTA 
TACCGCCTCG CTATCCAAAG CTTTGCCAGC GCACGTCCAT TTCTAACTAC TGAATGTGAA 
GATGTTCTCT TAGTGCTTGG CAGATTAGTC CTGAGTTGTT TTGAATTACT GCTTTCAGTG 
TCTGAAAGTG AACTGCCCTG TGAAGTCTGG GTACCATTCC TTCAGTCTCT ACAGGAGTCA 
CATGATGCAT TGTTGGAATT TGGAAATAAT AATTTACAAA TACTGGTTCA TGTTACAAAA 
GAAGGAGTGT GGAAAAACCC CATTCTTCTT AAAATTCTGT CACAACAGCC AGTAGAGACA 
GAGGAAGTTA ACAAATTGAT TGCACAAGAA GGACCTTCCT TTCTACAAAT GAGAATCAAG 
CATTTGTTGA AGTCTAACTG CATCCCCCAA GCTACTGCTT TATCAAAACT GTGTGCAGAG 
TCTAAAGAAC TTGCCCAGGT GTCGTGTTTT CAGCAAGCCT ATATCACATG TTTATGTTCC 
ATGCTTCCCA GCGAAGAGGC TATTCAGGAG ATTGCAAAGG TGGACTGCAA GGACGTGTTA 
GACATCATAT GTAATCTGGA ATCTGAGGGT CAGGATAACA CAGCATTTGT TCTTTGTACA 
ACTTACCTTA CCCAGCAGCT CCAAACGGCC AGTGTATATT GTTCTTGGGA GCTGACTCTC 
TTTTGGAGTA AACTGCAAAG AAGAATTGAC CCATCTTTGG AGACATTTTT GGAGCGTTGT 
CGGCAGTTTG GTGTCATAGC TAAAACACAA CAGCATTTAT TTTGCCTGAT TAGAGTCATA 
CAGACTGAAG CACAAGATGC TGGCATTGGG GTATCAATTT TATTATGTGT CAGAGCTCTT 
CAACTCAGAT CAAGTGAAGA TGAGGAAATG AAAGCTTCGG TCTGTAAAAC AATTTCCTGT 
CTTTTACCAG AAGATTTGGA AGTCAGACGA GCCTGTCAGC TTACAGAATT CTTAATTGAA 
CCCAGTTTGG ATGGATTCAA TATGCTGGAA GAACTGTATT TGCAGCCAGA TCAAAAATTT 
GATGAAGAAA ACGCACCAGT TCCAAATTCC CTGCGGTGTG AGCTTTTACT AGCTTTAAAA 
GCCCATTGGC CCTTTGATCC TGAATTTTGG GACTGGAAAA CTTTGAAGCG ACACTGCCAC 
CAACTCCTGG GACAAGAAGC CTCAGATTCC GATGATGACC TAAGTGGCTA TGAAATGTCT 
ATTAATGATA CAGATGTCTT AGAGTCATTT CTCAGTGACT ATGATGATGG AAAAGAAGAT 
AAGCAATATA GAAGAAGTCT AACAGATCAG AATAAGGAGA AAAGAGACAA AAAGCCCATT 
GGTTCCTCTG AGAGATACCA GAGGTGGCTT CAGTATAAGT TTTTCTGTTT GTTGTGTAAG 
CGGGAATGCA TAGAGGCCAG GATTCTCCAT CATTCCAAGA TGCACATGGA AGATGGAATA 
TATACCTGTC CAGTTTGTAT TAAAAAATTC AAGAGAAAAG AGCTATTTGT TCCTCACGTA 
ATGGAACATG TTAAAATGCC ACCAAGCAGA AGCCACCGTT CTAGAAAGAA ATTACTATTG 
AAAAGCGCTC AAAGGGGTAT TTATCCCAAG AGTCCTACTG GAAGTCTGGA ACAAAATCCA 
GAGCAAGCCA GGGGAGAGTC TCATGAATAT GTCACATTTA GCAAGTTAGA AGACCGCCGC 
CTGCAAGACA GAGACTTGTA CCCGTGTCCG GGTACAGATT GTTCCCGTGT GTTCAAACAG 
TTTAAATATT TAAGCGTGCA TCTTAAAGCT GAACACCAAA ATAATGACGA AAACGCCAAG 
CACTACTTGG ATATGAAAAA TAGGAGAGAG AAGTGTACTT ATTGCCGGCG GCATTTCATG 
TCAGCTTTTC ACCTCCGAGA ACATGAGCAG GTGCATTGTG GTCCTCAGCC TTACATGTGT 
GTCTCTATAG ATTGCTATGC AAGGTTTGGG TCAGTGAATG AACTCCTTAA CCACAAGCAG 
AAGCATGATG ACCTCCGTTA CAAATGTGAG TTGAATGGCT GCAATATTGT GTTCAGTGAC 
TTGGGCCAGC TTTACCACCA TGAAGCACAA CACTTCAGGG ATGCATCCTA CACATGCAAT 
GTCCTTGGCT GTAAAAAGTT CTATTATTCT AAAATTGAAT ACCAGAACCA CCTCTCCATG 
CATAATGTTG AAAGTCCAGA TGGAGAATTA AAGAAATCAG TGAAACTTGA GGAACCTGGA 
GCAGGTGGGA AGCAAGATTG TGTGGACCAG TCCCATCTAC TTGATGAAAC TGAGAAGTCC 
CATTCTCTTG AAGATCACCA TCTCTGTCCA GGGTCAGCTA GTGCTCATAT AGACACCACA 
GAGACCCTGA AGGACAACAG CGACAGCAAT TCTAGTGATC AGTTAAGTCA CAGCTCTTCC 
ACGTCAATAA CTGAGGAGTT AATTGACACC CTAGACCACT CTGAAACCAT GCAGGACCTG 
TTACTGTCTC ATGAGAAAGT CTTTGTTCCC TCCAGTTTAA AAGACAAGTG CTCCAATGTG 
GCTGTTTGTT TTGATGGAAC TAAATTCACC TGTGGTTTTG ACGGCTGTGG CTCCACCTAC 
AAAAACGCAA GAGGGATGCA GAAACATCTG CGGAAGGTGC ATCCGTACCA CTGCAAGCCA 
AGAAAAATAA AGACAAAAGA CCTCTTTAAC TGCCTGGATG ACAAACATAA TGAAGCTGAC 
AAGTTTGATG CAGAACCTAA ACCTAGCTCT GATACAAATA GTGACTCCCC AGATGAAGGT 
CCAGATCACA GTATCCACAC AAAATGCAAA CGAGAACATC AAGGTTATTC CCCTGAACCT 
TCCATTTGTG CTTCTAAAAG GCCATGTACA GAGGATACAA TGTTGGAACT TCTGTTACGT 
TTGAAACATT TAAGCTTGAA GAACTCAATA GCACATGGCT CTTTCTCAGG GTCATTGCAA 
GGGTGCCCAT CTAGTGGTGC TAAGTCTCTT CAGTCAGTCC CATCTTCCAT TTCAGACGTT 
AATCTTCAGA ATCAGGATGA AAATATGCCA AGTCAGTACC TTGCACAGTT GGCGGCAAAG 
CCTTTCTTCT GTGAGCTTCA AGGATGCAAA TATGAGTTTG TGACCAGAGA AGCTTTGTTA 
ATGCATTATC TTAAAAAGCA TAACTATTCA AAAGAGAAGG TCCTTCAGTT AACCATGTTT 
CAGCACCGAT ATTCCCCATT CCGGTGTCAC ATCTGCCAAA GGTCATTTAC AAGAAAAACA 
CATCTTAGGA TTCATTATAA AAACAAACAT CAAATTGGCA GTGACAGGGC AACTCACAGA 
CTGTTAGACA GTGAAAAATG TGATCATGAA GGGCCATGCT CAGTAGACAG ACTGAAAGGT 
GACTGCTCTA CAGAACTTGG TCCCAACAGT AATTCAGAGA CCACGCAATG TCATTCTAAA 
AAAGATGAAT GCAGCTCAGA AACAGACTTG GAGTCCTCTT GTGAGGAAAC AGAAAGTAAG 
ATCTCTGGTA TCTCATCACC AATAGGTAGC CATAGAGAAG AGGGAGAGGA GAAAGAGGGG 
AGAGGAAGTA GGCGGACTGT TGCTAAAGGA AATCTGTGCT ATATTTTGAA TAAGTACCAC 
AAACCATTCC ATTGTATCCA CAAAACTTGC AATTCTTCAT TTACCAATCT AAAAGGCCTG 
ATTCGCCATT ACAGAACTGT GCATCAGTAC AACAAAGAGC AGTTATGCTT AGAGAAGGAC 
AAAGCAAGAA CCAAAAGGGA GCTTGTAAAA TGTAAAAAGC TATTTGCTTG CAAATATAAA 
GACTGTAACA AGCGCTTCCT GTGCTCCAAG GCCCTGGCTA AGCACTGTAG TGACTCTCAT 
AACCTAGACC ACATTGAAGA GTCTAAAGTG CTTTCTGAGA CGGAGTCTGC AGCGAGGTTT 
TCTTGTAACC AGCCTCAGTG CCCTGCTGTT TTTTATTCAT TCAGTAAGTT GAAACACCAC 
CTGTTGGAAC AGCATAATAT TGAAGGAGAA ATCCATTCGG ATTATGAGAT CCATTGTACT 
CTTAATGGCT GTGGCCAGAT TTTCAGCCAT CGAAGTAATT ACTTCCAGCA TGTCTACTAC 
CGACATAAGG ACTATTATGA CAACCTGTTC AGCAGCCAGA AAGTAGCAAA TGAGCGGCTT 
CTAAGGAGTG AAAAGGTGTG TCAGACAACT CAGGCACAAG GACTGACGCA GACGCAGGGG 
CAGACACAGG GACAGACGCA GGGACAGGAA CAGCAGGCTG CCAAAAGGCC ATTTAATACT 
AAAGCTAAAA AATGTGGCTT ACTCAAAGAT AAAAAAGCTC CAATTACCTT TAAAACAAGA 
GCAGAAGCGA TCCATATGTG TGTTGAGCAT TCTGAGCACA CACAGTACCC GTGCATGGTT 
CAGGGATGCT TGTCTGTGGT GAAGCTGGAA AGCAGCATTG TGAGACATTA CAAACGTACC 
CACCAGATGA ATAGTGCCTA TTTAGAGCAG CAGTTGGAGA ACCTTGTGGT TTGTGTTAAG 
TATGGTACCA AAATTAAAGA CGAGCCCCCT TCAGAAGTAG AGCCCTGTGT AAAGAAAGAG 
GAAAGTACTA GCTGTGAATC GGTGCACACA GAGAATGGAG CCCCAGGAGA CAGCAGTGTG 
CCCCTCCCAA ACACTGATTC TACTTGCCCA GCTGAACAAG ATGTCGGTCA GAAAGGGTGT 
TCAGAAAGAA ACCCAGTTTT TGACACACAC AGTCTGCTCT ACAGGGGAAC TTTGAAATGC 
AACCATTCTT CAGAAACCAC TTCTTTGGAA CAGTGTAATA TAGCTCAGTC TTCTCCCTGT 
AAAATAGAAA GCCCCATACC TAATCCCAGT GGGACAGAAA GTGGTACTTA TTTCACGGAC 
TTCCAGCTGC CATTACCAAG GATCAAAGAA GAACCTGGGC AGCATAGTTC AGGGCAAGAA 
AACACTGTAA AGAATGCAAC CCAGGTCCCA AAAGAGAATA TTAGAAAGCA TTCTCAGCCC 
AGGTCATTTG ATCTGAAGAC TTACAAACCT ATGGGATTTG AGTCTTCCTT TCTGAAATTT 
ATTCAGGAAA GTGAAGAGAA GGATGATGAT TTTGATGACT GGGAGCCTTC AGAGCACTTA 
ACATTGAATA ACTCCTCTCA CCCCAGTAAT GACTTGACAG GGAATGTTGT GGCAGATACC 
ATAGTGAATG AGAGTGCCCC TCAAGTTGAC ATACCTCATT CTTCCAGTGA CCCTCCAGTT 
TCAGAGAACC TGACTGCAGT CCCACCATTA GTAGTAGCTG AAGCAACAGC AGTTCCTTCC 
TTGGAAAACC TGAGGGTTGT GCTGGACAAA GCATTAACAG ACTGTGGAGA ACTTGCCTTA 
AAACAGCTTC ATTACCTTCG GCCTGTGGTT GTCCTTGAAA GATCTAAGTT CTCTACACCA 
ATTTTAGACT TGTTTCCAAC TAAGAAGACA GATGAGCTTT GTGTAGGAAG TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333589.1
CDS	14..5770
Translation	MADGKGDATAAAGAGAGAEAPAVAGAEVETESMARGHRPASPASGAPGLRPCLWQLETELREQEVSEVSSLSYCRSFCQTLLQYASNKNASEHIVYLLEVYRLAIQSFASARPFLTTECEDVLLVLGRLVLSCFELLLSVSESELPCEVWVPFLQSLQESHDALLEFGNNNLQILVHVTKEGVWKNPILLKILSQQPVETEEVNKLIAQEGPSFLQMRIKHLLKSNCIPQATALSKLCAESKELAQVSCFQQAYITCLCSMLPSEEAIQEIAKVDCKDVLDIICNLESEGQDNTAFVLCTTYLTQQLQTASVYCSWELTLFWSKLQRRIDPSLETFLERCRQFGVIAKTQQHLFCLIRVIQTEAQDAGIGVSILLCVRALQLRSSEDEEMKASVCKTISCLLPEDLEVRRACQLTEFLIEPSLDGFNMLEELYLQPDQKFDEENAPVPNSLRCELLLALKAHWPFDPEFWDWKTLKRHCHQLLGQEASDSDDDLSGYEMSINDTDVLESFLSDYDDGKEDKQYRRSLTDQNKEKRDKKPIGSSERYQRWLQYKFFCLLCKRECIEARILHHSKMHMEDGIYTCPVCIKKFKRKELFVPHVMEHVKMPPSRSHRSRKKLLLKSAQRGIYPKSPTGSLEQNPEQARGESHEYVTFSKLEDRRLQDRDLYPCPGTDCSRVFKQFKYLSVHLKAEHQNNDENAKHYLDMKNRREKCTYCRRHFMSAFHLREHEQVHCGPQPYMCVSIDCYARFGSVNELLNHKQKHDDLRYKCELNGCNIVFSDLGQLYHHEAQHFRDASYTCNVLGCKKFYYSKIEYQNHLSMHNVESPDGELKKSVKLEEPGAGGKQDCVDQSHLLDETEKSHSLEDHHLCPGSASAHIDTTETLKDNSDSNSSDQLSHSSSTSITEELIDTLDHSETMQDLLLSHEKVFVPSSLKDKCSNVAVCFDGTKFTCGFDGCGSTYKNARGMQKHLRKVHPYHCKPRKIKTKDLFNCLDDKHNEADKFDAEPKPSSDTNSDSPDEGPDHSIHTKCKREHQGYSPEPSICASKRPCTEDTMLELLLRLKHLSLKNSIAHGSFSGSLQGCPSSGAKSLQSVPSSISDVNLQNQDENMPSQYLAQLAAKPFFCELQGCKYEFVTREALLMHYLKKHNYSKEKVLQLTMFQHRYSPFRCHICQRSFTRKTHLRIHYKNKHQIGSDRATHRLLDSEKCDHEGPCSVDRLKGDCSTELGPNSNSETTQCHSKKDECSSETDLESSCEETESKISGISSPIGSHREEGEEKEGRGSRRTVAKGNLCYILNKYHKPFHCIHKTCNSSFTNLKGLIRHYRTVHQYNKEQLCLEKDKARTKRELVKCKKLFACKYKDCNKRFLCSKALAKHCSDSHNLDHIEESKVLSETESAARFSCNQPQCPAVFYSFSKLKHHLLEQHNIEGEIHSDYEIHCTLNGCGQIFSHRSNYFQHVYYRHKDYYDNLFSSQKVANERLLRSEKVCQTTQAQGLTQTQGQTQGQTQGQEQQAAKRPFNTKAKKCGLLKDKKAPITFKTRAEAIHMCVEHSEHTQYPCMVQGCLSVVKLESSIVRHYKRTHQMNSAYLEQQLENLVVCVKYGTKIKDEPPSEVEPCVKKEESTSCESVHTENGAPGDSSVPLPNTDSTCPAEQDVGQKGCSERNPVFDTHSLLYRGTLKCNHSSETTSLEQCNIAQSSPCKIESPIPNPSGTESGTYFTDFQLPLPRIKEEPGQHSSGQENTVKNATQVPKENIRKHSQPRSFDLKTYKPMGFESSFLKFIQESEEKDDDFDDWEPSEHLTLNNSSHPSNDLTGNVVADTIVNESAPQVDIPHSSSDPPVSENLTAVPPLVVAEATAVPSLENLRVVLDKALTDCGELALKQLHYLRPVVVLERSKFSTPILDLFPTKKTDELCVGSS

Target ORF information:

RefSeq Version	NM_001346660.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus rearranged L-myc fusion sequence (Rlf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346660.1

ORF Insert Sequence:

ATGGCGGACG GAAAGGGAGA CGCCACGGCC GCCGCCGGGG CCGGGGCTGG GGCTGAGGCT 
CCGGCGGTGG CGGGAGCCGA AGTCGAGACT GAGTCCATGG CTCGGGGTCA TCGCCCTGCA 
TCTCCGGCGT CGGGAGCGCC GGGCCTACGA CCGTGTCTCT GGCAGCTGGA GACCGAGCTG 
AGGGAGCAGG AGGTGTCCGA GGTCTCATCT TTGAGCTACT GCCGGAGCTT CTGCCAGACC 
TTATTGCAAT ATGCAAGCAA CAAGAATGCA TCAGAACACA TTGTGTATCT TCTGGAGGTA 
TACCGCCTCG CTATCCAAAG CTTTGCCAGC GCACGTCCAT TTCTAACTAC TGAATGTGAA 
GATGTTCTCT TAGTGCTTGG CAGATTAGTC CTGAGTTGTT TTGAATTACT GCTTTCAGTG 
TCTGAAAGTG AACTGCCCTG TGAAGTCTGG GTACCATTCC TTCAGTCTCT ACAGGAGTCA 
CATGATGCAT TGTTGGAATT TGGAAATAAT AATTTACAAA TACTGGTTCA TGTTACAAAA 
GAAGGAGTGT GGAAAAACCC CATTCTTCTT AAAATTCTGT CACAACAGCC AGTAGAGACA 
GAGGAAGTTA ACAAATTGAT TGCACAAGAA GGACCTTCCT TTCTACAAAT GAGAATCAAG 
CATTTGTTGA AGTCTAACTG CATCCCCCAA GCTACTGCTT TATCAAAACT GTGTGCAGAG 
TCTAAAGAAC TTGCCCAGGT GTCGTGTTTT CAGCAAGCCT ATATCACATG TTTATGTTCC 
ATGCTTCCCA GCGAAGAGGC TATTCAGGAG ATTGCAAAGG TGGACTGCAA GGACGTGTTA 
GACATCATAT GTAATCTGGA ATCTGAGGGT CAGGATAACA CAGCATTTGT TCTTTGTACA 
ACTTACCTTA CCCAGCAGCT CCAAACGGCC AGTGTATATT GTTCTTGGGA GCTGACTCTC 
TTTTGGAGTA AACTGCAAAG AAGAATTGAC CCATCTTTGG AGACATTTTT GGAGCGTTGT 
CGGCAGTTTG GTGTCATAGC TAAAACACAA CAGCATTTAT TTTGCCTGAT TAGAGTCATA 
CAGACTGAAG CACAAGATGC TGGCATTGGG GTATCAATTT TATTATGTGT CAGAGCTCTT 
CAACTCAGAT CAAGTGAAGA TGAGGAAATG AAAGCTTCGG TCTGTAAAAC AATTTCCTGT 
CTTTTACCAG AAGATTTGGA AGTCAGACGA GCCTGTCAGC TTACAGAATT CTTAATTGAA 
CCCAGTTTGG ATGGATTCAA TATGCTGGAA GAACTGTATT TGCAGCCAGA TCAAAAATTT 
GATGAAGAAA ACGCACCAGT TCCAAATTCC CTGCGGTGTG AGCTTTTACT AGCTTTAAAA 
GCCCATTGGC CCTTTGATCC TGAATTTTGG GACTGGAAAA CTTTGAAGCG ACACTGCCAC 
CAACTCCTGG GACAAGAAGC CTCAGATTCC GATGATGACC TAAGTGGCTA TGAAATGTCT 
ATTAATGATA CAGATGTCTT AGAGTCATTT CTCAGTGACT ATGATGATGG AAAAGAAGAT 
AAGCAATATA GAAGAAGTCT AACAGATCAG AATAAGGAGA AAAGAGACAA AAAGCCCATT 
GGTTCCTCTG AGAGATACCA GAGGTGGCTT CAGTATAAGT TTTTCTGTTT GTTGTGTAAG 
CGGGAATGCA TAGAGGCCAG GATTCTCCAT CATTCCAAGA TGCACATGGA AGATGGAATA 
TATACCTGTC CAGTTTGTAT TAAAAAATTC AAGAGAAAAG AGCTATTTGT TCCTCACGTA 
ATGGAACATG TTAAAATGCC ACCAAGCAGA AGCCACCGTT CTAGAAAGAA ATTACTATTG 
AAAAGCGCTC AAAGGGGTAT TTATCCCAAG AGTCCTACTG GAAGTCTGGA ACAAAATCCA 
GAGCAAGCCA GGGGAGAGTC TCATGAATAT GTCACATTTA GCAAGTTAGA AGACCGCCGC 
CTGCAAGACA GAGACTTGTA CCCGTGTCCG GGTACAGATT GTTCCCGTGT GTTCAAACAG 
TTTAAATATT TAAGCGTGCA TCTTAAAGCT GAACACCAAA ATAATGACGA AAACGCCAAG 
CACTACTTGG ATATGAAAAA TAGGAGAGAG AAGTGTACTT ATTGCCGGCG GCATTTCATG 
TCAGCTTTTC ACCTCCGAGA ACATGAGCAG GTGCATTGTG GTCCTCAGCC TTACATGTGT 
GTCTCTATAG ATTGCTATGC AAGGTTTGGG TCAGTGAATG AACTCCTTAA CCACAAGCAG 
AAGCATGATG ACCTCCGTTA CAAATGTGAG TTGAATGGCT GCAATATTGT GTTCAGTGAC 
TTGGGCCAGC TTTACCACCA TGAAGCACAA CACTTCAGGG ATGCATCCTA CACATGCAAT 
GTCCTTGGCT GTAAAAAGTT CTATTATTCT AAAATTGAAT ACCAGAACCA CCTCTCCATG 
CATAATGTTG AAAGTCCAGA TGGAGAATTA AAGAAATCAG TGAAACTTGA GGAACCTGGA 
GCAGGTGGGA AGCAAGATTG TGTGGACCAG TCCCATCTAC TTGATGAAAC TGAGAAGTCC 
CATTCTCTTG AAGATCACCA TCTCTGTCCA GGGTCAGCTA GTGCTCATAT AGACACCACA 
GAGACCCTGA AGGACAACAG CGACAGCAAT TCTAGTGATC AGTTAAGTCA CAGCTCTTCC 
ACGTCAATAA CTGAGGAGTT AATTGACACC CTAGACCACT CTGAAACCAT GCAGGACCTG 
TTACTGTCTC ATGAGAAAGT CTTTGTTCCC TCCAGTTTAA AAGACAAGTG CTCCAATGTG 
GCTGTTTGTT TTGATGGAAC TAAATTCACC TGTGGTTTTG ACGGCTGTGG CTCCACCTAC 
AAAAACGCAA GAGGGATGCA GAAACATCTG CGGAAGGTGC ATCCGTACCA CTGCAAGCCA 
AGAAAAATAA AGACAAAAGA CCTCTTTAAC TGCCTGGATG ACAAACATAA TGAAGCTGAC 
AAGTTTGATG CAGAACCTAA ACCTAGCTCT GATACAAATA GTGACTCCCC AGATGAAGGT 
CCAGATCACA GTATCCACAC AAAATGCAAA CGAGAACATC AAGGTTATTC CCCTGAACCT 
TCCATTTGTG CTTCTAAAAG GCCATGTACA GAGGATACAA TGTTGGAACT TCTGTTACGT 
TTGAAACATT TAAGCTTGAA GAACTCAATA GCACATGGCT CTTTCTCAGG GTCATTGCAA 
GGGTGCCCAT CTAGTGGTGC TAAGTCTCTT CAGTCAGTCC CATCTTCCAT TTCAGACGTT 
AATCTTCAGA ATCAGGATGA AAATATGCCA AGTCAGTACC TTGCACAGTT GGCGGCAAAG 
CCTTTCTTCT GTGAGCTTCA AGGATGCAAA TATGAGTTTG TGACCAGAGA AGCTTTGTTA 
ATGCATTATC TTAAAAAGCA TAACTATTCA AAAGAGAAGG TCCTTCAGTT AACCATGTTT 
CAGCACCGAT ATTCCCCATT CCGGTGTCAC ATCTGCCAAA GGTCATTTAC AAGAAAAACA 
CATCTTAGGA TTCATTATAA AAACAAACAT CAAATTGGCA GTGACAGGGC AACTCACAGA 
CTGTTAGACA GTGAAAAATG TGATCATGAA GGGCCATGCT CAGTAGACAG ACTGAAAGGT 
GACTGCTCTA CAGAACTTGG TCCCAACAGT AATTCAGAGA CCACGCAATG TCATTCTAAA 
AAAGATGAAT GCAGCTCAGA AACAGACTTG GAGTCCTCTT GTGAGGAAAC AGAAAGTAAG 
ATCTCTGGTA TCTCATCACC AATAGGTAGC CATAGAGAAG AGGGAGAGGA GAAAGAGGGG 
AGAGGAAGTA GGCGGACTGT TGCTAAAGGA AATCTGTGCT ATATTTTGAA TAAGTACCAC 
AAACCATTCC ATTGTATCCA CAAAACTTGC AATTCTTCAT TTACCAATCT AAAAGGCCTG 
ATTCGCCATT ACAGAACTGT GCATCAGTAC AACAAAGAGC AGTTATGCTT AGAGAAGGAC 
AAAGCAAGAA CCAAAAGGGA GCTTGTAAAA TGTAAAAAGC TATTTGCTTG CAAATATAAA 
GACTGTAACA AGCGCTTCCT GTGCTCCAAG GCCCTGGCTA AGCACTGTAG TGACTCTCAT 
AACCTAGACC ACATTGAAGA GTCTAAAGTG CTTTCTGAGA CGGAGTCTGC AGCGAGGTTT 
TCTTGTAACC AGCCTCAGTG CCCTGCTGTT TTTTATTCAT TCAGTAAGTT GAAACACCAC 
CTGTTGGAAC AGCATAATAT TGAAGGAGAA ATCCATTCGG ATTATGAGAT CCATTGTACT 
CTTAATGGCT GTGGCCAGAT TTTCAGCCAT CGAAGTAATT ACTTCCAGCA TGTCTACTAC 
CGACATAAGG ACTATTATGA CAACCTGTTC AGCAGCCAGA AAGTAGCAAA TGAGCGGCTT 
CTAAGGAGTG AAAAGGTGTG TCAGACAACT CAGGCACAAG GACTGACGCA GACGCAGGGG 
CAGACACAGG GACAGACGCA GGGACAGGAA CAGCAGGCTG CCAAAAGGCC ATTTAATACT 
AAAGCTAAAA AATGTGGCTT ACTCAAAGAT AAAAAAGCTC CAATTACCTT TAAAACAAGA 
GCAGAAGCGA TCCATATGTG TGTTGAGCAT TCTGAGCACA CACAGTACCC GTGCATGGTT 
CAGGGATGCT TGTCTGTGGT GAAGCTGGAA AGCAGCATTG TGAGACATTA CAAACGTACC 
CACCAGATGA ATAGTGCCTA TTTAGAGCAG CAGTTGGAGA ACCTTGTGGT TTGTGTTAAG 
TATGGTACCA AAATTAAAGA CGAGCCCCCT TCAGAAGTAG AGCCCTGTGT AAAGAAAGAG 
GAAAGTACTA GCTGTGAATC GGTGCACACA GAGAATGGAG CCCCAGGAGA CAGCAGTGTG 
CCCCTCCCAA ACACTGATTC TACTTGCCCA GCTGAACAAG ATGTCGGTCA GAAAGGGTGT 
TCAGAAAGAA ACCCAGTTTT TGACACACAC AGTCTGCTCT ACAGGGGAAC TTTGAAATGC 
AACCATTCTT CAGAAACCAC TTCTTTGGAA CAGTGTAATA TAGCTCAGTC TTCTCCCTGT 
AAAATAGAAA GCCCCATACC TAATCCCAGT GGGACAGAAA GTGGTACTTA TTTCACGGAC 
TTCCAGCTGC CATTACCAAG GATCAAAGAA GAACCTGGGC AGCATAGTTC AGGGCAAGAA 
AACACTGTAA AGAATGCAAC CCAGGTCCCA AAAGAGAATA TTAGAAAGCA TTCTCAGCCC 
AGGTCATTTG ATCTGAAGAC TTACAAACCT ATGGGATTTG AGTCTTCCTT TCTGAAATTT 
ATTCAGGAAA GTGAAGAGAA GGATGATGAT TTTGATGACT GGGAGCCTTC AGAGCACTTA 
ACATTGAATA ACTCCTCTCA CCCCAGTAAT GACTTGACAG GGAATGTTGT GGCAGATACC 
ATAGTGAATG AGAGTGCCCC TCAAGTTGAC ATACCTCATT CTTCCAGTGA CCCTCCAGTT 
TCAGAGAACC TGACTGCAGT CCCACCATTA GTAGTAGCTG AAGCAACAGC AGTTCCTTCC 
TTGGAAAACC TGAGGGTTGT GCTGGACAAA GCATTAACAG ACTGTGGAGA ACTTGCCTTA 
AAACAGCTTC ATTACCTTCG GCCTGTGGTT GTCCTTGAAA GATCTAAGTT CTCTACACCA 
ATTTTAGACT TGTTTCCAAC TAAGAAGACA GATGAGCTTT GTGTAGGAAG TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rlf ( XM_006502657.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006502720.1 Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X3, mRNA.

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CloneID	OMu33281
Clone ID Related Accession (Same CDS sequence)	XM_006502657.4 , XM_017319915.1 , XM_017319915.2 , XM_006502657.3 , XM_006502658.1 , XM_006502658.2 , XM_030253080.1
Accession Version	XM_006502657.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	zinc finger protein Rlf isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006502657.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATCA AGCATTTGTT GAAGTCTAAC TGCATCCCCC AAGCTACTGC TTTATCAAAA 
CTGTGTGCAG AGTCTAAAGA ACTTGCCCAG GTGTCGTGTT TTCAGCAAGC CTATATCACA 
TGTTTATGTT CCATGCTTCC CAGCGAAGAG GCTATTCAGG AGATTGCAAA GGTGGACTGC 
AAGGACGTGT TAGACATCAT ATGTAATCTG GAATCTGAGG GTCAGGATAA CACAGCATTT 
GTTCTTTGTA CAACTTACCT TACCCAGCAG CTCCAAACGG CCAGTGTATA TTGTTCTTGG 
GAGCTGACTC TCTTTTGGAG TAAACTGCAA AGAAGAATTG ACCCATCTTT GGAGACATTT 
TTGGAGCGTT GTCGGCAGTT TGGTGTCATA GCTAAAACAC AACAGCATTT ATTTTGCCTG 
ATTAGAGTCA TACAGACTGA AGCACAAGAT GCTGGCATTG GGGTATCAAT TTTATTATGT 
GTCAGAGCTC TTCAACTCAG ATCAAGTGAA GATGAGGAAA TGAAAGCTTC GGTCTGTAAA 
ACAATTTCCT GTCTTTTACC AGAAGATTTG GAAGTCAGAC GAGCCTGTCA GCTTACAGAA 
TTCTTAATTG AACCCAGTTT GGATGGATTC AATATGCTGG AAGAACTGTA TTTGCAGCCA 
GATCAAAAAT TTGATGAAGA AAACGCACCA GTTCCAAATT CCCTGCGGTG TGAGCTTTTA 
CTAGCTTTAA AAGCCCATTG GCCCTTTGAT CCTGAATTTT GGGACTGGAA AACTTTGAAG 
CGACACTGCC ACCAACTCCT GGGACAAGAA GCCTCAGATT CCGATGATGA CCTAAGTGGC 
TATGAAATGT CTATTAATGA TACAGATGTC TTAGAGTCAT TTCTCAGTGA CTATGATGAT 
GGAAAAGAAG ATAAGCAATA TAGAAGAAGT CTAACAGATC AGAATAAGGA GAAAAGAGAC 
AAAAAGCCCA TTGGTTCCTC TGAGAGATAC CAGAGGTGGC TTCAGTATAA GTTTTTCTGT 
TTGTTGTGTA AGCGGGAATG CATAGAGGCC AGGATTCTCC ATCATTCCAA GATGCACATG 
GAAGATGGAA TATATACCTG TCCAGTTTGT ATTAAAAAAT TCAAGAGAAA AGAGCTATTT 
GTTCCTCACG TAATGGAACA TGTTAAAATG CCACCAAGCA GAAGCCACCG TTCTAGAAAG 
AAATTACTAT TGAAAAGCGC TCAAAGGGGT ATTTATCCCA AGAGTCCTAC TGGAAGTCTG 
GAACAAAATC CAGAGCAAGC CAGGGGAGAG TCTCATGAAT ATGTCACATT TAGCAAGTTA 
GAAGACCGCC GCCTGCAAGA CAGAGACTTG TACCCGTGTC CGGGTACAGA TTGTTCCCGT 
GTGTTCAAAC AGTTTAAATA TTTAAGCGTG CATCTTAAAG CTGAACACCA AAATAATGAC 
GAAAACGCCA AGCACTACTT GGATATGAAA AATAGGAGAG AGAAGTGTAC TTATTGCCGG 
CGGCATTTCA TGTCAGCTTT TCACCTCCGA GAACATGAGC AGGTGCATTG TGGTCCTCAG 
CCTTACATGT GTGTCTCTAT AGATTGCTAT GCAAGGTTTG GGTCAGTGAA TGAACTCCTT 
AACCACAAGC AGAAGCATGA TGACCTCCGT TACAAATGTG AGTTGAATGG CTGCAATATT 
GTGTTCAGTG ACTTGGGCCA GCTTTACCAC CATGAAGCAC AACACTTCAG GGATGCATCC 
TACACATGCA ATGTCCTTGG CTGTAAAAAG TTCTATTATT CTAAAATTGA ATACCAGAAC 
CACCTCTCCA TGCATAATGT TGAAAGTCCA GATGGAGAAT TAAAGAAATC AGTGAAACTT 
GAGGAACCTG GAGCAGGTGG GAAGCAAGAT TGTGTGGACC AGTCCCATCT ACTTGATGAA 
ACTGAGAAGT CCCATTCTCT TGAAGATCAC CATCTCTGTC CAGGGTCAGC TAGTGCTCAT 
ATAGACACCA CAGAGACCCT GAAGGACAAC AGCGACAGCA ATTCTAGTGA TCAGTTAAGT 
CACAGCTCTT CCACGTCAAT AACTGAGGAG TTAATTGACA CCCTAGACCA CTCTGAAACC 
ATGCAGGACC TGTTACTGTC TCATGAGAAA GTCTTTGTTC CCTCCAGTTT AAAAGACAAG 
TGCTCCAATG TGGCTGTTTG TTTTGATGGA ACTAAATTCA CCTGTGGTTT TGACGGCTGT 
GGCTCCACCT ACAAAAACGC AAGAGGGATG CAGAAACATC TGCGGAAGGT GCATCCGTAC 
CACTGCAAGC CAAGAAAAAT AAAGACAAAA GACCTCTTTA ACTGCCTGGA TGACAAACAT 
AATGAAGCTG ACAAGTTTGA TGCAGAACCT AAACCTAGCT CTGATACAAA TAGTGACTCC 
CCAGATGAAG GTCCAGATCA CAGTATCCAC ACAAAATGCA AACGAGAACA TCAAGGTTAT 
TCCCCTGAAC CTTCCATTTG TGCTTCTAAA AGGCCATGTA CAGAGGATAC AATGTTGGAA 
CTTCTGTTAC GTTTGAAACA TTTAAGCTTG AAGAACTCAA TAGCACATGG CTCTTTCTCA 
GGGTCATTGC AAGGGTGCCC ATCTAGTGGT GCTAAGTCTC TTCAGTCAGT CCCATCTTCC 
ATTTCAGACG TTAATCTTCA GAATCAGGAT GAAAATATGC CAAGTCAGTA CCTTGCACAG 
TTGGCGGCAA AGCCTTTCTT CTGTGAGCTT CAAGGATGCA AATATGAGTT TGTGACCAGA 
GAAGCTTTGT TAATGCATTA TCTTAAAAAG CATAACTATT CAAAAGAGAA GGTCCTTCAG 
TTAACCATGT TTCAGCACCG ATATTCCCCA TTCCGGTGTC ACATCTGCCA AAGGTCATTT 
ACAAGAAAAA CACATCTTAG GATTCATTAT AAAAACAAAC ATCAAATTGG CAGTGACAGG 
GCAACTCACA GACTGTTAGA CAGTGAAAAA TGTGATCATG AAGGGCCATG CTCAGTAGAC 
AGACTGAAAG GTGACTGCTC TACAGAACTT GGTCCCAACA GTAATTCAGA GACCACGCAA 
TGTCATTCTA AAAAAGATGA ATGCAGCTCA GAAACAGACT TGGAGTCCTC TTGTGAGGAA 
ACAGAAAGTA AGATCTCTGG TATCTCATCA CCAATAGGTA GCCATAGAGA AGAGGGAGAG 
GAGAAAGAGG GGAGAGGAAG TAGGCGGACT GTTGCTAAAG GAAATCTGTG CTATATTTTG 
AATAAGTACC ACAAACCATT CCATTGTATC CACAAAACTT GCAATTCTTC ATTTACCAAT 
CTAAAAGGCC TGATTCGCCA TTACAGAACT GTGCATCAGT ACAACAAAGA GCAGTTATGC 
TTAGAGAAGG ACAAAGCAAG AACCAAAAGG GAGCTTGTAA AATGTAAAAA GCTATTTGCT 
TGCAAATATA AAGACTGTAA CAAGCGCTTC CTGTGCTCCA AGGCCCTGGC TAAGCACTGT 
AGTGACTCTC ATAACCTAGA CCACATTGAA GAGTCTAAAG TGCTTTCTGA GACGGAGTCT 
GCAGCGAGGT TTTCTTGTAA CCAGCCTCAG TGCCCTGCTG TTTTTTATTC ATTCAGTAAG 
TTGAAACACC ACCTGTTGGA ACAGCATAAT ATTGAAGGAG AAATCCATTC GGATTATGAG 
ATCCATTGTA CTCTTAATGG CTGTGGCCAG ATTTTCAGCC ATCGAAGTAA TTACTTCCAG 
CATGTCTACT ACCGACATAA GGACTATTAT GACAACCTGT TCAGCAGCCA GAAAGTAGCA 
AATGAGCGGC TTCTAAGGAG TGAAAAGGTG TGTCAGACAA CTCAGGCACA AGGACTGACG 
CAGACGCAGG GGCAGACACA GGGACAGACG CAGGGACAGG AACAGCAGGC TGCCAAAAGG 
CCATTTAATA CTAAAGCTAA AAAATGTGGC TTACTCAAAG ATAAAAAAGC TCCAATTACC 
TTTAAAACAA GAGCAGAAGC GATCCATATG TGTGTTGAGC ATTCTGAGCA CACACAGTAC 
CCGTGCATGG TTCAGGGATG CTTGTCTGTG GTGAAGCTGG AAAGCAGCAT TGTGAGACAT 
TACAAACGTA CCCACCAGAT GAATAGTGCC TATTTAGAGC AGCAGTTGGA GAACCTTGTG 
GTTTGTGTTA AGTATGGTAC CAAAATTAAA GACGAGCCCC CTTCAGAAGT AGAGCCCTGT 
GTAAAGAAAG AGGAAAGTAC TAGCTGTGAA TCGGTGCACA CAGAGAATGG AGCCCCAGGA 
GACAGCAGTG TGCCCCTCCC AAACACTGAT TCTACTTGCC CAGCTGAACA AGATGTCGGT 
CAGAAAGGGT GTTCAGAAAG AAACCCAGTT TTTGACACAC ACAGTCTGCT CTACAGGGGA 
ACTTTGAAAT GCAACCATTC TTCAGAAACC ACTTCTTTGG AACAGTGTAA TATAGCTCAG 
TCTTCTCCCT GTAAAATAGA AAGCCCCATA CCTAATCCCA GTGGGACAGA AAGTGGTACT 
TATTTCACGG ACTTCCAGCT GCCATTACCA AGGATCAAAG AAGAACCTGG GCAGCATAGT 
TCAGGGCAAG AAAACACTGT AAAGAATGCA ACCCAGGTCC CAAAAGAGAA TATTAGAAAG 
CATTCTCAGC CCAGGTCATT TGATCTGAAG ACTTACAAAC CTATGGGATT TGAGTCTTCC 
TTTCTGAAAT TTATTCAGGA AAGTGAAGAG AAGGATGATG ATTTTGATGA CTGGGAGCCT 
TCAGAGCACT TAACATTGAA TAACTCCTCT CACCCCAGTA ATGACTTGAC AGGGAATGTT 
GTGGCAGATA CCATAGTGAA TGAGAGTGCC CCTCAAGTTG ACATACCTCA TTCTTCCAGT 
GACCCTCCAG TTTCAGAGAA CCTGACTGCA GTCCCACCAT TAGTAGTAGC TGAAGCAACA 
GCAGTTCCTT CCTTGGAAAA CCTGAGGGTT GTGCTGGACA AAGCATTAAC AGACTGTGGA 
GAACTTGCCT TAAAACAGCT TCATTACCTT CGGCCTGTGG TTGTCCTTGA AAGATCTAAG 
TTCTCTACAC CAATTTTAGA CTTGTTTCCA ACTAAGAAGA CAGATGAGCT TTGTGTAGGA 
AGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006502720.1
CDS	2595..7703
Translation	MRIKHLLKSNCIPQATALSKLCAESKELAQVSCFQQAYITCLCSMLPSEEAIQEIAKVDCKDVLDIICNLESEGQDNTAFVLCTTYLTQQLQTASVYCSWELTLFWSKLQRRIDPSLETFLERCRQFGVIAKTQQHLFCLIRVIQTEAQDAGIGVSILLCVRALQLRSSEDEEMKASVCKTISCLLPEDLEVRRACQLTEFLIEPSLDGFNMLEELYLQPDQKFDEENAPVPNSLRCELLLALKAHWPFDPEFWDWKTLKRHCHQLLGQEASDSDDDLSGYEMSINDTDVLESFLSDYDDGKEDKQYRRSLTDQNKEKRDKKPIGSSERYQRWLQYKFFCLLCKRECIEARILHHSKMHMEDGIYTCPVCIKKFKRKELFVPHVMEHVKMPPSRSHRSRKKLLLKSAQRGIYPKSPTGSLEQNPEQARGESHEYVTFSKLEDRRLQDRDLYPCPGTDCSRVFKQFKYLSVHLKAEHQNNDENAKHYLDMKNRREKCTYCRRHFMSAFHLREHEQVHCGPQPYMCVSIDCYARFGSVNELLNHKQKHDDLRYKCELNGCNIVFSDLGQLYHHEAQHFRDASYTCNVLGCKKFYYSKIEYQNHLSMHNVESPDGELKKSVKLEEPGAGGKQDCVDQSHLLDETEKSHSLEDHHLCPGSASAHIDTTETLKDNSDSNSSDQLSHSSSTSITEELIDTLDHSETMQDLLLSHEKVFVPSSLKDKCSNVAVCFDGTKFTCGFDGCGSTYKNARGMQKHLRKVHPYHCKPRKIKTKDLFNCLDDKHNEADKFDAEPKPSSDTNSDSPDEGPDHSIHTKCKREHQGYSPEPSICASKRPCTEDTMLELLLRLKHLSLKNSIAHGSFSGSLQGCPSSGAKSLQSVPSSISDVNLQNQDENMPSQYLAQLAAKPFFCELQGCKYEFVTREALLMHYLKKHNYSKEKVLQLTMFQHRYSPFRCHICQRSFTRKTHLRIHYKNKHQIGSDRATHRLLDSEKCDHEGPCSVDRLKGDCSTELGPNSNSETTQCHSKKDECSSETDLESSCEETESKISGISSPIGSHREEGEEKEGRGSRRTVAKGNLCYILNKYHKPFHCIHKTCNSSFTNLKGLIRHYRTVHQYNKEQLCLEKDKARTKRELVKCKKLFACKYKDCNKRFLCSKALAKHCSDSHNLDHIEESKVLSETESAARFSCNQPQCPAVFYSFSKLKHHLLEQHNIEGEIHSDYEIHCTLNGCGQIFSHRSNYFQHVYYRHKDYYDNLFSSQKVANERLLRSEKVCQTTQAQGLTQTQGQTQGQTQGQEQQAAKRPFNTKAKKCGLLKDKKAPITFKTRAEAIHMCVEHSEHTQYPCMVQGCLSVVKLESSIVRHYKRTHQMNSAYLEQQLENLVVCVKYGTKIKDEPPSEVEPCVKKEESTSCESVHTENGAPGDSSVPLPNTDSTCPAEQDVGQKGCSERNPVFDTHSLLYRGTLKCNHSSETTSLEQCNIAQSSPCKIESPIPNPSGTESGTYFTDFQLPLPRIKEEPGQHSSGQENTVKNATQVPKENIRKHSQPRSFDLKTYKPMGFESSFLKFIQESEEKDDDFDDWEPSEHLTLNNSSHPSNDLTGNVVADTIVNESAPQVDIPHSSSDPPVSENLTAVPPLVVAEATAVPSLENLRVVLDKALTDCGELALKQLHYLRPVVVLERSKFSTPILDLFPTKKTDELCVGSS

Target ORF information:

RefSeq Version	XM_006502657.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006502657.4

ORF Insert Sequence:

ATGAGAATCA AGCATTTGTT GAAGTCTAAC TGCATCCCCC AAGCTACTGC TTTATCAAAA 
CTGTGTGCAG AGTCTAAAGA ACTTGCCCAG GTGTCGTGTT TTCAGCAAGC CTATATCACA 
TGTTTATGTT CCATGCTTCC CAGCGAAGAG GCTATTCAGG AGATTGCAAA GGTGGACTGC 
AAGGACGTGT TAGACATCAT ATGTAATCTG GAATCTGAGG GTCAGGATAA CACAGCATTT 
GTTCTTTGTA CAACTTACCT TACCCAGCAG CTCCAAACGG CCAGTGTATA TTGTTCTTGG 
GAGCTGACTC TCTTTTGGAG TAAACTGCAA AGAAGAATTG ACCCATCTTT GGAGACATTT 
TTGGAGCGTT GTCGGCAGTT TGGTGTCATA GCTAAAACAC AACAGCATTT ATTTTGCCTG 
ATTAGAGTCA TACAGACTGA AGCACAAGAT GCTGGCATTG GGGTATCAAT TTTATTATGT 
GTCAGAGCTC TTCAACTCAG ATCAAGTGAA GATGAGGAAA TGAAAGCTTC GGTCTGTAAA 
ACAATTTCCT GTCTTTTACC AGAAGATTTG GAAGTCAGAC GAGCCTGTCA GCTTACAGAA 
TTCTTAATTG AACCCAGTTT GGATGGATTC AATATGCTGG AAGAACTGTA TTTGCAGCCA 
GATCAAAAAT TTGATGAAGA AAACGCACCA GTTCCAAATT CCCTGCGGTG TGAGCTTTTA 
CTAGCTTTAA AAGCCCATTG GCCCTTTGAT CCTGAATTTT GGGACTGGAA AACTTTGAAG 
CGACACTGCC ACCAACTCCT GGGACAAGAA GCCTCAGATT CCGATGATGA CCTAAGTGGC 
TATGAAATGT CTATTAATGA TACAGATGTC TTAGAGTCAT TTCTCAGTGA CTATGATGAT 
GGAAAAGAAG ATAAGCAATA TAGAAGAAGT CTAACAGATC AGAATAAGGA GAAAAGAGAC 
AAAAAGCCCA TTGGTTCCTC TGAGAGATAC CAGAGGTGGC TTCAGTATAA GTTTTTCTGT 
TTGTTGTGTA AGCGGGAATG CATAGAGGCC AGGATTCTCC ATCATTCCAA GATGCACATG 
GAAGATGGAA TATATACCTG TCCAGTTTGT ATTAAAAAAT TCAAGAGAAA AGAGCTATTT 
GTTCCTCACG TAATGGAACA TGTTAAAATG CCACCAAGCA GAAGCCACCG TTCTAGAAAG 
AAATTACTAT TGAAAAGCGC TCAAAGGGGT ATTTATCCCA AGAGTCCTAC TGGAAGTCTG 
GAACAAAATC CAGAGCAAGC CAGGGGAGAG TCTCATGAAT ATGTCACATT TAGCAAGTTA 
GAAGACCGCC GCCTGCAAGA CAGAGACTTG TACCCGTGTC CGGGTACAGA TTGTTCCCGT 
GTGTTCAAAC AGTTTAAATA TTTAAGCGTG CATCTTAAAG CTGAACACCA AAATAATGAC 
GAAAACGCCA AGCACTACTT GGATATGAAA AATAGGAGAG AGAAGTGTAC TTATTGCCGG 
CGGCATTTCA TGTCAGCTTT TCACCTCCGA GAACATGAGC AGGTGCATTG TGGTCCTCAG 
CCTTACATGT GTGTCTCTAT AGATTGCTAT GCAAGGTTTG GGTCAGTGAA TGAACTCCTT 
AACCACAAGC AGAAGCATGA TGACCTCCGT TACAAATGTG AGTTGAATGG CTGCAATATT 
GTGTTCAGTG ACTTGGGCCA GCTTTACCAC CATGAAGCAC AACACTTCAG GGATGCATCC 
TACACATGCA ATGTCCTTGG CTGTAAAAAG TTCTATTATT CTAAAATTGA ATACCAGAAC 
CACCTCTCCA TGCATAATGT TGAAAGTCCA GATGGAGAAT TAAAGAAATC AGTGAAACTT 
GAGGAACCTG GAGCAGGTGG GAAGCAAGAT TGTGTGGACC AGTCCCATCT ACTTGATGAA 
ACTGAGAAGT CCCATTCTCT TGAAGATCAC CATCTCTGTC CAGGGTCAGC TAGTGCTCAT 
ATAGACACCA CAGAGACCCT GAAGGACAAC AGCGACAGCA ATTCTAGTGA TCAGTTAAGT 
CACAGCTCTT CCACGTCAAT AACTGAGGAG TTAATTGACA CCCTAGACCA CTCTGAAACC 
ATGCAGGACC TGTTACTGTC TCATGAGAAA GTCTTTGTTC CCTCCAGTTT AAAAGACAAG 
TGCTCCAATG TGGCTGTTTG TTTTGATGGA ACTAAATTCA CCTGTGGTTT TGACGGCTGT 
GGCTCCACCT ACAAAAACGC AAGAGGGATG CAGAAACATC TGCGGAAGGT GCATCCGTAC 
CACTGCAAGC CAAGAAAAAT AAAGACAAAA GACCTCTTTA ACTGCCTGGA TGACAAACAT 
AATGAAGCTG ACAAGTTTGA TGCAGAACCT AAACCTAGCT CTGATACAAA TAGTGACTCC 
CCAGATGAAG GTCCAGATCA CAGTATCCAC ACAAAATGCA AACGAGAACA TCAAGGTTAT 
TCCCCTGAAC CTTCCATTTG TGCTTCTAAA AGGCCATGTA CAGAGGATAC AATGTTGGAA 
CTTCTGTTAC GTTTGAAACA TTTAAGCTTG AAGAACTCAA TAGCACATGG CTCTTTCTCA 
GGGTCATTGC AAGGGTGCCC ATCTAGTGGT GCTAAGTCTC TTCAGTCAGT CCCATCTTCC 
ATTTCAGACG TTAATCTTCA GAATCAGGAT GAAAATATGC CAAGTCAGTA CCTTGCACAG 
TTGGCGGCAA AGCCTTTCTT CTGTGAGCTT CAAGGATGCA AATATGAGTT TGTGACCAGA 
GAAGCTTTGT TAATGCATTA TCTTAAAAAG CATAACTATT CAAAAGAGAA GGTCCTTCAG 
TTAACCATGT TTCAGCACCG ATATTCCCCA TTCCGGTGTC ACATCTGCCA AAGGTCATTT 
ACAAGAAAAA CACATCTTAG GATTCATTAT AAAAACAAAC ATCAAATTGG CAGTGACAGG 
GCAACTCACA GACTGTTAGA CAGTGAAAAA TGTGATCATG AAGGGCCATG CTCAGTAGAC 
AGACTGAAAG GTGACTGCTC TACAGAACTT GGTCCCAACA GTAATTCAGA GACCACGCAA 
TGTCATTCTA AAAAAGATGA ATGCAGCTCA GAAACAGACT TGGAGTCCTC TTGTGAGGAA 
ACAGAAAGTA AGATCTCTGG TATCTCATCA CCAATAGGTA GCCATAGAGA AGAGGGAGAG 
GAGAAAGAGG GGAGAGGAAG TAGGCGGACT GTTGCTAAAG GAAATCTGTG CTATATTTTG 
AATAAGTACC ACAAACCATT CCATTGTATC CACAAAACTT GCAATTCTTC ATTTACCAAT 
CTAAAAGGCC TGATTCGCCA TTACAGAACT GTGCATCAGT ACAACAAAGA GCAGTTATGC 
TTAGAGAAGG ACAAAGCAAG AACCAAAAGG GAGCTTGTAA AATGTAAAAA GCTATTTGCT 
TGCAAATATA AAGACTGTAA CAAGCGCTTC CTGTGCTCCA AGGCCCTGGC TAAGCACTGT 
AGTGACTCTC ATAACCTAGA CCACATTGAA GAGTCTAAAG TGCTTTCTGA GACGGAGTCT 
GCAGCGAGGT TTTCTTGTAA CCAGCCTCAG TGCCCTGCTG TTTTTTATTC ATTCAGTAAG 
TTGAAACACC ACCTGTTGGA ACAGCATAAT ATTGAAGGAG AAATCCATTC GGATTATGAG 
ATCCATTGTA CTCTTAATGG CTGTGGCCAG ATTTTCAGCC ATCGAAGTAA TTACTTCCAG 
CATGTCTACT ACCGACATAA GGACTATTAT GACAACCTGT TCAGCAGCCA GAAAGTAGCA 
AATGAGCGGC TTCTAAGGAG TGAAAAGGTG TGTCAGACAA CTCAGGCACA AGGACTGACG 
CAGACGCAGG GGCAGACACA GGGACAGACG CAGGGACAGG AACAGCAGGC TGCCAAAAGG 
CCATTTAATA CTAAAGCTAA AAAATGTGGC TTACTCAAAG ATAAAAAAGC TCCAATTACC 
TTTAAAACAA GAGCAGAAGC GATCCATATG TGTGTTGAGC ATTCTGAGCA CACACAGTAC 
CCGTGCATGG TTCAGGGATG CTTGTCTGTG GTGAAGCTGG AAAGCAGCAT TGTGAGACAT 
TACAAACGTA CCCACCAGAT GAATAGTGCC TATTTAGAGC AGCAGTTGGA GAACCTTGTG 
GTTTGTGTTA AGTATGGTAC CAAAATTAAA GACGAGCCCC CTTCAGAAGT AGAGCCCTGT 
GTAAAGAAAG AGGAAAGTAC TAGCTGTGAA TCGGTGCACA CAGAGAATGG AGCCCCAGGA 
GACAGCAGTG TGCCCCTCCC AAACACTGAT TCTACTTGCC CAGCTGAACA AGATGTCGGT 
CAGAAAGGGT GTTCAGAAAG AAACCCAGTT TTTGACACAC ACAGTCTGCT CTACAGGGGA 
ACTTTGAAAT GCAACCATTC TTCAGAAACC ACTTCTTTGG AACAGTGTAA TATAGCTCAG 
TCTTCTCCCT GTAAAATAGA AAGCCCCATA CCTAATCCCA GTGGGACAGA AAGTGGTACT 
TATTTCACGG ACTTCCAGCT GCCATTACCA AGGATCAAAG AAGAACCTGG GCAGCATAGT 
TCAGGGCAAG AAAACACTGT AAAGAATGCA ACCCAGGTCC CAAAAGAGAA TATTAGAAAG 
CATTCTCAGC CCAGGTCATT TGATCTGAAG ACTTACAAAC CTATGGGATT TGAGTCTTCC 
TTTCTGAAAT TTATTCAGGA AAGTGAAGAG AAGGATGATG ATTTTGATGA CTGGGAGCCT 
TCAGAGCACT TAACATTGAA TAACTCCTCT CACCCCAGTA ATGACTTGAC AGGGAATGTT 
GTGGCAGATA CCATAGTGAA TGAGAGTGCC CCTCAAGTTG ACATACCTCA TTCTTCCAGT 
GACCCTCCAG TTTCAGAGAA CCTGACTGCA GTCCCACCAT TAGTAGTAGC TGAAGCAACA 
GCAGTTCCTT CCTTGGAAAA CCTGAGGGTT GTGCTGGACA AAGCATTAAC AGACTGTGGA 
GAACTTGCCT TAAAACAGCT TCATTACCTT CGGCCTGTGG TTGTCCTTGA AAGATCTAAG 
TTCTCTACAC CAATTTTAGA CTTGTTTCCA ACTAAGAAGA CAGATGAGCT TTGTGTAGGA 
AGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rlf ( XM_017319915.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175404.1 Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu33281
Clone ID Related Accession (Same CDS sequence)	XM_006502657.4 , XM_017319915.1 , XM_017319915.2 , XM_006502657.3 , XM_006502658.1 , XM_006502658.2 , XM_030253080.1
Accession Version	XM_017319915.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	zinc finger protein Rlf isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATCA AGCATTTGTT GAAGTCTAAC TGCATCCCCC AAGCTACTGC TTTATCAAAA 
CTGTGTGCAG AGTCTAAAGA ACTTGCCCAG GTGTCGTGTT TTCAGCAAGC CTATATCACA 
TGTTTATGTT CCATGCTTCC CAGCGAAGAG GCTATTCAGG AGATTGCAAA GGTGGACTGC 
AAGGACGTGT TAGACATCAT ATGTAATCTG GAATCTGAGG GTCAGGATAA CACAGCATTT 
GTTCTTTGTA CAACTTACCT TACCCAGCAG CTCCAAACGG CCAGTGTATA TTGTTCTTGG 
GAGCTGACTC TCTTTTGGAG TAAACTGCAA AGAAGAATTG ACCCATCTTT GGAGACATTT 
TTGGAGCGTT GTCGGCAGTT TGGTGTCATA GCTAAAACAC AACAGCATTT ATTTTGCCTG 
ATTAGAGTCA TACAGACTGA AGCACAAGAT GCTGGCATTG GGGTATCAAT TTTATTATGT 
GTCAGAGCTC TTCAACTCAG ATCAAGTGAA GATGAGGAAA TGAAAGCTTC GGTCTGTAAA 
ACAATTTCCT GTCTTTTACC AGAAGATTTG GAAGTCAGAC GAGCCTGTCA GCTTACAGAA 
TTCTTAATTG AACCCAGTTT GGATGGATTC AATATGCTGG AAGAACTGTA TTTGCAGCCA 
GATCAAAAAT TTGATGAAGA AAACGCACCA GTTCCAAATT CCCTGCGGTG TGAGCTTTTA 
CTAGCTTTAA AAGCCCATTG GCCCTTTGAT CCTGAATTTT GGGACTGGAA AACTTTGAAG 
CGACACTGCC ACCAACTCCT GGGACAAGAA GCCTCAGATT CCGATGATGA CCTAAGTGGC 
TATGAAATGT CTATTAATGA TACAGATGTC TTAGAGTCAT TTCTCAGTGA CTATGATGAT 
GGAAAAGAAG ATAAGCAATA TAGAAGAAGT CTAACAGATC AGAATAAGGA GAAAAGAGAC 
AAAAAGCCCA TTGGTTCCTC TGAGAGATAC CAGAGGTGGC TTCAGTATAA GTTTTTCTGT 
TTGTTGTGTA AGCGGGAATG CATAGAGGCC AGGATTCTCC ATCATTCCAA GATGCACATG 
GAAGATGGAA TATATACCTG TCCAGTTTGT ATTAAAAAAT TCAAGAGAAA AGAGCTATTT 
GTTCCTCACG TAATGGAACA TGTTAAAATG CCACCAAGCA GAAGCCACCG TTCTAGAAAG 
AAATTACTAT TGAAAAGCGC TCAAAGGGGT ATTTATCCCA AGAGTCCTAC TGGAAGTCTG 
GAACAAAATC CAGAGCAAGC CAGGGGAGAG TCTCATGAAT ATGTCACATT TAGCAAGTTA 
GAAGACCGCC GCCTGCAAGA CAGAGACTTG TACCCGTGTC CGGGTACAGA TTGTTCCCGT 
GTGTTCAAAC AGTTTAAATA TTTAAGCGTG CATCTTAAAG CTGAACACCA AAATAATGAC 
GAAAACGCCA AGCACTACTT GGATATGAAA AATAGGAGAG AGAAGTGTAC TTATTGCCGG 
CGGCATTTCA TGTCAGCTTT TCACCTCCGA GAACATGAGC AGGTGCATTG TGGTCCTCAG 
CCTTACATGT GTGTCTCTAT AGATTGCTAT GCAAGGTTTG GGTCAGTGAA TGAACTCCTT 
AACCACAAGC AGAAGCATGA TGACCTCCGT TACAAATGTG AGTTGAATGG CTGCAATATT 
GTGTTCAGTG ACTTGGGCCA GCTTTACCAC CATGAAGCAC AACACTTCAG GGATGCATCC 
TACACATGCA ATGTCCTTGG CTGTAAAAAG TTCTATTATT CTAAAATTGA ATACCAGAAC 
CACCTCTCCA TGCATAATGT TGAAAGTCCA GATGGAGAAT TAAAGAAATC AGTGAAACTT 
GAGGAACCTG GAGCAGGTGG GAAGCAAGAT TGTGTGGACC AGTCCCATCT ACTTGATGAA 
ACTGAGAAGT CCCATTCTCT TGAAGATCAC CATCTCTGTC CAGGGTCAGC TAGTGCTCAT 
ATAGACACCA CAGAGACCCT GAAGGACAAC AGCGACAGCA ATTCTAGTGA TCAGTTAAGT 
CACAGCTCTT CCACGTCAAT AACTGAGGAG TTAATTGACA CCCTAGACCA CTCTGAAACC 
ATGCAGGACC TGTTACTGTC TCATGAGAAA GTCTTTGTTC CCTCCAGTTT AAAAGACAAG 
TGCTCCAATG TGGCTGTTTG TTTTGATGGA ACTAAATTCA CCTGTGGTTT TGACGGCTGT 
GGCTCCACCT ACAAAAACGC AAGAGGGATG CAGAAACATC TGCGGAAGGT GCATCCGTAC 
CACTGCAAGC CAAGAAAAAT AAAGACAAAA GACCTCTTTA ACTGCCTGGA TGACAAACAT 
AATGAAGCTG ACAAGTTTGA TGCAGAACCT AAACCTAGCT CTGATACAAA TAGTGACTCC 
CCAGATGAAG GTCCAGATCA CAGTATCCAC ACAAAATGCA AACGAGAACA TCAAGGTTAT 
TCCCCTGAAC CTTCCATTTG TGCTTCTAAA AGGCCATGTA CAGAGGATAC AATGTTGGAA 
CTTCTGTTAC GTTTGAAACA TTTAAGCTTG AAGAACTCAA TAGCACATGG CTCTTTCTCA 
GGGTCATTGC AAGGGTGCCC ATCTAGTGGT GCTAAGTCTC TTCAGTCAGT CCCATCTTCC 
ATTTCAGACG TTAATCTTCA GAATCAGGAT GAAAATATGC CAAGTCAGTA CCTTGCACAG 
TTGGCGGCAA AGCCTTTCTT CTGTGAGCTT CAAGGATGCA AATATGAGTT TGTGACCAGA 
GAAGCTTTGT TAATGCATTA TCTTAAAAAG CATAACTATT CAAAAGAGAA GGTCCTTCAG 
TTAACCATGT TTCAGCACCG ATATTCCCCA TTCCGGTGTC ACATCTGCCA AAGGTCATTT 
ACAAGAAAAA CACATCTTAG GATTCATTAT AAAAACAAAC ATCAAATTGG CAGTGACAGG 
GCAACTCACA GACTGTTAGA CAGTGAAAAA TGTGATCATG AAGGGCCATG CTCAGTAGAC 
AGACTGAAAG GTGACTGCTC TACAGAACTT GGTCCCAACA GTAATTCAGA GACCACGCAA 
TGTCATTCTA AAAAAGATGA ATGCAGCTCA GAAACAGACT TGGAGTCCTC TTGTGAGGAA 
ACAGAAAGTA AGATCTCTGG TATCTCATCA CCAATAGGTA GCCATAGAGA AGAGGGAGAG 
GAGAAAGAGG GGAGAGGAAG TAGGCGGACT GTTGCTAAAG GAAATCTGTG CTATATTTTG 
AATAAGTACC ACAAACCATT CCATTGTATC CACAAAACTT GCAATTCTTC ATTTACCAAT 
CTAAAAGGCC TGATTCGCCA TTACAGAACT GTGCATCAGT ACAACAAAGA GCAGTTATGC 
TTAGAGAAGG ACAAAGCAAG AACCAAAAGG GAGCTTGTAA AATGTAAAAA GCTATTTGCT 
TGCAAATATA AAGACTGTAA CAAGCGCTTC CTGTGCTCCA AGGCCCTGGC TAAGCACTGT 
AGTGACTCTC ATAACCTAGA CCACATTGAA GAGTCTAAAG TGCTTTCTGA GACGGAGTCT 
GCAGCGAGGT TTTCTTGTAA CCAGCCTCAG TGCCCTGCTG TTTTTTATTC ATTCAGTAAG 
TTGAAACACC ACCTGTTGGA ACAGCATAAT ATTGAAGGAG AAATCCATTC GGATTATGAG 
ATCCATTGTA CTCTTAATGG CTGTGGCCAG ATTTTCAGCC ATCGAAGTAA TTACTTCCAG 
CATGTCTACT ACCGACATAA GGACTATTAT GACAACCTGT TCAGCAGCCA GAAAGTAGCA 
AATGAGCGGC TTCTAAGGAG TGAAAAGGTG TGTCAGACAA CTCAGGCACA AGGACTGACG 
CAGACGCAGG GGCAGACACA GGGACAGACG CAGGGACAGG AACAGCAGGC TGCCAAAAGG 
CCATTTAATA CTAAAGCTAA AAAATGTGGC TTACTCAAAG ATAAAAAAGC TCCAATTACC 
TTTAAAACAA GAGCAGAAGC GATCCATATG TGTGTTGAGC ATTCTGAGCA CACACAGTAC 
CCGTGCATGG TTCAGGGATG CTTGTCTGTG GTGAAGCTGG AAAGCAGCAT TGTGAGACAT 
TACAAACGTA CCCACCAGAT GAATAGTGCC TATTTAGAGC AGCAGTTGGA GAACCTTGTG 
GTTTGTGTTA AGTATGGTAC CAAAATTAAA GACGAGCCCC CTTCAGAAGT AGAGCCCTGT 
GTAAAGAAAG AGGAAAGTAC TAGCTGTGAA TCGGTGCACA CAGAGAATGG AGCCCCAGGA 
GACAGCAGTG TGCCCCTCCC AAACACTGAT TCTACTTGCC CAGCTGAACA AGATGTCGGT 
CAGAAAGGGT GTTCAGAAAG AAACCCAGTT TTTGACACAC ACAGTCTGCT CTACAGGGGA 
ACTTTGAAAT GCAACCATTC TTCAGAAACC ACTTCTTTGG AACAGTGTAA TATAGCTCAG 
TCTTCTCCCT GTAAAATAGA AAGCCCCATA CCTAATCCCA GTGGGACAGA AAGTGGTACT 
TATTTCACGG ACTTCCAGCT GCCATTACCA AGGATCAAAG AAGAACCTGG GCAGCATAGT 
TCAGGGCAAG AAAACACTGT AAAGAATGCA ACCCAGGTCC CAAAAGAGAA TATTAGAAAG 
CATTCTCAGC CCAGGTCATT TGATCTGAAG ACTTACAAAC CTATGGGATT TGAGTCTTCC 
TTTCTGAAAT TTATTCAGGA AAGTGAAGAG AAGGATGATG ATTTTGATGA CTGGGAGCCT 
TCAGAGCACT TAACATTGAA TAACTCCTCT CACCCCAGTA ATGACTTGAC AGGGAATGTT 
GTGGCAGATA CCATAGTGAA TGAGAGTGCC CCTCAAGTTG ACATACCTCA TTCTTCCAGT 
GACCCTCCAG TTTCAGAGAA CCTGACTGCA GTCCCACCAT TAGTAGTAGC TGAAGCAACA 
GCAGTTCCTT CCTTGGAAAA CCTGAGGGTT GTGCTGGACA AAGCATTAAC AGACTGTGGA 
GAACTTGCCT TAAAACAGCT TCATTACCTT CGGCCTGTGG TTGTCCTTGA AAGATCTAAG 
TTCTCTACAC CAATTTTAGA CTTGTTTCCA ACTAAGAAGA CAGATGAGCT TTGTGTAGGA 
AGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175404.1
CDS	1485..6593
Translation	MRIKHLLKSNCIPQATALSKLCAESKELAQVSCFQQAYITCLCSMLPSEEAIQEIAKVDCKDVLDIICNLESEGQDNTAFVLCTTYLTQQLQTASVYCSWELTLFWSKLQRRIDPSLETFLERCRQFGVIAKTQQHLFCLIRVIQTEAQDAGIGVSILLCVRALQLRSSEDEEMKASVCKTISCLLPEDLEVRRACQLTEFLIEPSLDGFNMLEELYLQPDQKFDEENAPVPNSLRCELLLALKAHWPFDPEFWDWKTLKRHCHQLLGQEASDSDDDLSGYEMSINDTDVLESFLSDYDDGKEDKQYRRSLTDQNKEKRDKKPIGSSERYQRWLQYKFFCLLCKRECIEARILHHSKMHMEDGIYTCPVCIKKFKRKELFVPHVMEHVKMPPSRSHRSRKKLLLKSAQRGIYPKSPTGSLEQNPEQARGESHEYVTFSKLEDRRLQDRDLYPCPGTDCSRVFKQFKYLSVHLKAEHQNNDENAKHYLDMKNRREKCTYCRRHFMSAFHLREHEQVHCGPQPYMCVSIDCYARFGSVNELLNHKQKHDDLRYKCELNGCNIVFSDLGQLYHHEAQHFRDASYTCNVLGCKKFYYSKIEYQNHLSMHNVESPDGELKKSVKLEEPGAGGKQDCVDQSHLLDETEKSHSLEDHHLCPGSASAHIDTTETLKDNSDSNSSDQLSHSSSTSITEELIDTLDHSETMQDLLLSHEKVFVPSSLKDKCSNVAVCFDGTKFTCGFDGCGSTYKNARGMQKHLRKVHPYHCKPRKIKTKDLFNCLDDKHNEADKFDAEPKPSSDTNSDSPDEGPDHSIHTKCKREHQGYSPEPSICASKRPCTEDTMLELLLRLKHLSLKNSIAHGSFSGSLQGCPSSGAKSLQSVPSSISDVNLQNQDENMPSQYLAQLAAKPFFCELQGCKYEFVTREALLMHYLKKHNYSKEKVLQLTMFQHRYSPFRCHICQRSFTRKTHLRIHYKNKHQIGSDRATHRLLDSEKCDHEGPCSVDRLKGDCSTELGPNSNSETTQCHSKKDECSSETDLESSCEETESKISGISSPIGSHREEGEEKEGRGSRRTVAKGNLCYILNKYHKPFHCIHKTCNSSFTNLKGLIRHYRTVHQYNKEQLCLEKDKARTKRELVKCKKLFACKYKDCNKRFLCSKALAKHCSDSHNLDHIEESKVLSETESAARFSCNQPQCPAVFYSFSKLKHHLLEQHNIEGEIHSDYEIHCTLNGCGQIFSHRSNYFQHVYYRHKDYYDNLFSSQKVANERLLRSEKVCQTTQAQGLTQTQGQTQGQTQGQEQQAAKRPFNTKAKKCGLLKDKKAPITFKTRAEAIHMCVEHSEHTQYPCMVQGCLSVVKLESSIVRHYKRTHQMNSAYLEQQLENLVVCVKYGTKIKDEPPSEVEPCVKKEESTSCESVHTENGAPGDSSVPLPNTDSTCPAEQDVGQKGCSERNPVFDTHSLLYRGTLKCNHSSETTSLEQCNIAQSSPCKIESPIPNPSGTESGTYFTDFQLPLPRIKEEPGQHSSGQENTVKNATQVPKENIRKHSQPRSFDLKTYKPMGFESSFLKFIQESEEKDDDFDDWEPSEHLTLNNSSHPSNDLTGNVVADTIVNESAPQVDIPHSSSDPPVSENLTAVPPLVVAEATAVPSLENLRVVLDKALTDCGELALKQLHYLRPVVVLERSKFSTPILDLFPTKKTDELCVGSS

Target ORF information:

RefSeq Version	XM_017319915.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319915.1

ORF Insert Sequence:

ATGAGAATCA AGCATTTGTT GAAGTCTAAC TGCATCCCCC AAGCTACTGC TTTATCAAAA 
CTGTGTGCAG AGTCTAAAGA ACTTGCCCAG GTGTCGTGTT TTCAGCAAGC CTATATCACA 
TGTTTATGTT CCATGCTTCC CAGCGAAGAG GCTATTCAGG AGATTGCAAA GGTGGACTGC 
AAGGACGTGT TAGACATCAT ATGTAATCTG GAATCTGAGG GTCAGGATAA CACAGCATTT 
GTTCTTTGTA CAACTTACCT TACCCAGCAG CTCCAAACGG CCAGTGTATA TTGTTCTTGG 
GAGCTGACTC TCTTTTGGAG TAAACTGCAA AGAAGAATTG ACCCATCTTT GGAGACATTT 
TTGGAGCGTT GTCGGCAGTT TGGTGTCATA GCTAAAACAC AACAGCATTT ATTTTGCCTG 
ATTAGAGTCA TACAGACTGA AGCACAAGAT GCTGGCATTG GGGTATCAAT TTTATTATGT 
GTCAGAGCTC TTCAACTCAG ATCAAGTGAA GATGAGGAAA TGAAAGCTTC GGTCTGTAAA 
ACAATTTCCT GTCTTTTACC AGAAGATTTG GAAGTCAGAC GAGCCTGTCA GCTTACAGAA 
TTCTTAATTG AACCCAGTTT GGATGGATTC AATATGCTGG AAGAACTGTA TTTGCAGCCA 
GATCAAAAAT TTGATGAAGA AAACGCACCA GTTCCAAATT CCCTGCGGTG TGAGCTTTTA 
CTAGCTTTAA AAGCCCATTG GCCCTTTGAT CCTGAATTTT GGGACTGGAA AACTTTGAAG 
CGACACTGCC ACCAACTCCT GGGACAAGAA GCCTCAGATT CCGATGATGA CCTAAGTGGC 
TATGAAATGT CTATTAATGA TACAGATGTC TTAGAGTCAT TTCTCAGTGA CTATGATGAT 
GGAAAAGAAG ATAAGCAATA TAGAAGAAGT CTAACAGATC AGAATAAGGA GAAAAGAGAC 
AAAAAGCCCA TTGGTTCCTC TGAGAGATAC CAGAGGTGGC TTCAGTATAA GTTTTTCTGT 
TTGTTGTGTA AGCGGGAATG CATAGAGGCC AGGATTCTCC ATCATTCCAA GATGCACATG 
GAAGATGGAA TATATACCTG TCCAGTTTGT ATTAAAAAAT TCAAGAGAAA AGAGCTATTT 
GTTCCTCACG TAATGGAACA TGTTAAAATG CCACCAAGCA GAAGCCACCG TTCTAGAAAG 
AAATTACTAT TGAAAAGCGC TCAAAGGGGT ATTTATCCCA AGAGTCCTAC TGGAAGTCTG 
GAACAAAATC CAGAGCAAGC CAGGGGAGAG TCTCATGAAT ATGTCACATT TAGCAAGTTA 
GAAGACCGCC GCCTGCAAGA CAGAGACTTG TACCCGTGTC CGGGTACAGA TTGTTCCCGT 
GTGTTCAAAC AGTTTAAATA TTTAAGCGTG CATCTTAAAG CTGAACACCA AAATAATGAC 
GAAAACGCCA AGCACTACTT GGATATGAAA AATAGGAGAG AGAAGTGTAC TTATTGCCGG 
CGGCATTTCA TGTCAGCTTT TCACCTCCGA GAACATGAGC AGGTGCATTG TGGTCCTCAG 
CCTTACATGT GTGTCTCTAT AGATTGCTAT GCAAGGTTTG GGTCAGTGAA TGAACTCCTT 
AACCACAAGC AGAAGCATGA TGACCTCCGT TACAAATGTG AGTTGAATGG CTGCAATATT 
GTGTTCAGTG ACTTGGGCCA GCTTTACCAC CATGAAGCAC AACACTTCAG GGATGCATCC 
TACACATGCA ATGTCCTTGG CTGTAAAAAG TTCTATTATT CTAAAATTGA ATACCAGAAC 
CACCTCTCCA TGCATAATGT TGAAAGTCCA GATGGAGAAT TAAAGAAATC AGTGAAACTT 
GAGGAACCTG GAGCAGGTGG GAAGCAAGAT TGTGTGGACC AGTCCCATCT ACTTGATGAA 
ACTGAGAAGT CCCATTCTCT TGAAGATCAC CATCTCTGTC CAGGGTCAGC TAGTGCTCAT 
ATAGACACCA CAGAGACCCT GAAGGACAAC AGCGACAGCA ATTCTAGTGA TCAGTTAAGT 
CACAGCTCTT CCACGTCAAT AACTGAGGAG TTAATTGACA CCCTAGACCA CTCTGAAACC 
ATGCAGGACC TGTTACTGTC TCATGAGAAA GTCTTTGTTC CCTCCAGTTT AAAAGACAAG 
TGCTCCAATG TGGCTGTTTG TTTTGATGGA ACTAAATTCA CCTGTGGTTT TGACGGCTGT 
GGCTCCACCT ACAAAAACGC AAGAGGGATG CAGAAACATC TGCGGAAGGT GCATCCGTAC 
CACTGCAAGC CAAGAAAAAT AAAGACAAAA GACCTCTTTA ACTGCCTGGA TGACAAACAT 
AATGAAGCTG ACAAGTTTGA TGCAGAACCT AAACCTAGCT CTGATACAAA TAGTGACTCC 
CCAGATGAAG GTCCAGATCA CAGTATCCAC ACAAAATGCA AACGAGAACA TCAAGGTTAT 
TCCCCTGAAC CTTCCATTTG TGCTTCTAAA AGGCCATGTA CAGAGGATAC AATGTTGGAA 
CTTCTGTTAC GTTTGAAACA TTTAAGCTTG AAGAACTCAA TAGCACATGG CTCTTTCTCA 
GGGTCATTGC AAGGGTGCCC ATCTAGTGGT GCTAAGTCTC TTCAGTCAGT CCCATCTTCC 
ATTTCAGACG TTAATCTTCA GAATCAGGAT GAAAATATGC CAAGTCAGTA CCTTGCACAG 
TTGGCGGCAA AGCCTTTCTT CTGTGAGCTT CAAGGATGCA AATATGAGTT TGTGACCAGA 
GAAGCTTTGT TAATGCATTA TCTTAAAAAG CATAACTATT CAAAAGAGAA GGTCCTTCAG 
TTAACCATGT TTCAGCACCG ATATTCCCCA TTCCGGTGTC ACATCTGCCA AAGGTCATTT 
ACAAGAAAAA CACATCTTAG GATTCATTAT AAAAACAAAC ATCAAATTGG CAGTGACAGG 
GCAACTCACA GACTGTTAGA CAGTGAAAAA TGTGATCATG AAGGGCCATG CTCAGTAGAC 
AGACTGAAAG GTGACTGCTC TACAGAACTT GGTCCCAACA GTAATTCAGA GACCACGCAA 
TGTCATTCTA AAAAAGATGA ATGCAGCTCA GAAACAGACT TGGAGTCCTC TTGTGAGGAA 
ACAGAAAGTA AGATCTCTGG TATCTCATCA CCAATAGGTA GCCATAGAGA AGAGGGAGAG 
GAGAAAGAGG GGAGAGGAAG TAGGCGGACT GTTGCTAAAG GAAATCTGTG CTATATTTTG 
AATAAGTACC ACAAACCATT CCATTGTATC CACAAAACTT GCAATTCTTC ATTTACCAAT 
CTAAAAGGCC TGATTCGCCA TTACAGAACT GTGCATCAGT ACAACAAAGA GCAGTTATGC 
TTAGAGAAGG ACAAAGCAAG AACCAAAAGG GAGCTTGTAA AATGTAAAAA GCTATTTGCT 
TGCAAATATA AAGACTGTAA CAAGCGCTTC CTGTGCTCCA AGGCCCTGGC TAAGCACTGT 
AGTGACTCTC ATAACCTAGA CCACATTGAA GAGTCTAAAG TGCTTTCTGA GACGGAGTCT 
GCAGCGAGGT TTTCTTGTAA CCAGCCTCAG TGCCCTGCTG TTTTTTATTC ATTCAGTAAG 
TTGAAACACC ACCTGTTGGA ACAGCATAAT ATTGAAGGAG AAATCCATTC GGATTATGAG 
ATCCATTGTA CTCTTAATGG CTGTGGCCAG ATTTTCAGCC ATCGAAGTAA TTACTTCCAG 
CATGTCTACT ACCGACATAA GGACTATTAT GACAACCTGT TCAGCAGCCA GAAAGTAGCA 
AATGAGCGGC TTCTAAGGAG TGAAAAGGTG TGTCAGACAA CTCAGGCACA AGGACTGACG 
CAGACGCAGG GGCAGACACA GGGACAGACG CAGGGACAGG AACAGCAGGC TGCCAAAAGG 
CCATTTAATA CTAAAGCTAA AAAATGTGGC TTACTCAAAG ATAAAAAAGC TCCAATTACC 
TTTAAAACAA GAGCAGAAGC GATCCATATG TGTGTTGAGC ATTCTGAGCA CACACAGTAC 
CCGTGCATGG TTCAGGGATG CTTGTCTGTG GTGAAGCTGG AAAGCAGCAT TGTGAGACAT 
TACAAACGTA CCCACCAGAT GAATAGTGCC TATTTAGAGC AGCAGTTGGA GAACCTTGTG 
GTTTGTGTTA AGTATGGTAC CAAAATTAAA GACGAGCCCC CTTCAGAAGT AGAGCCCTGT 
GTAAAGAAAG AGGAAAGTAC TAGCTGTGAA TCGGTGCACA CAGAGAATGG AGCCCCAGGA 
GACAGCAGTG TGCCCCTCCC AAACACTGAT TCTACTTGCC CAGCTGAACA AGATGTCGGT 
CAGAAAGGGT GTTCAGAAAG AAACCCAGTT TTTGACACAC ACAGTCTGCT CTACAGGGGA 
ACTTTGAAAT GCAACCATTC TTCAGAAACC ACTTCTTTGG AACAGTGTAA TATAGCTCAG 
TCTTCTCCCT GTAAAATAGA AAGCCCCATA CCTAATCCCA GTGGGACAGA AAGTGGTACT 
TATTTCACGG ACTTCCAGCT GCCATTACCA AGGATCAAAG AAGAACCTGG GCAGCATAGT 
TCAGGGCAAG AAAACACTGT AAAGAATGCA ACCCAGGTCC CAAAAGAGAA TATTAGAAAG 
CATTCTCAGC CCAGGTCATT TGATCTGAAG ACTTACAAAC CTATGGGATT TGAGTCTTCC 
TTTCTGAAAT TTATTCAGGA AAGTGAAGAG AAGGATGATG ATTTTGATGA CTGGGAGCCT 
TCAGAGCACT TAACATTGAA TAACTCCTCT CACCCCAGTA ATGACTTGAC AGGGAATGTT 
GTGGCAGATA CCATAGTGAA TGAGAGTGCC CCTCAAGTTG ACATACCTCA TTCTTCCAGT 
GACCCTCCAG TTTCAGAGAA CCTGACTGCA GTCCCACCAT TAGTAGTAGC TGAAGCAACA 
GCAGTTCCTT CCTTGGAAAA CCTGAGGGTT GTGCTGGACA AAGCATTAAC AGACTGTGGA 
GAACTTGCCT TAAAACAGCT TCATTACCTT CGGCCTGTGG TTGTCCTTGA AAGATCTAAG 
TTCTCTACAC CAATTTTAGA CTTGTTTCCA ACTAAGAAGA CAGATGAGCT TTGTGTAGGA 
AGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rlf ( XM_017319915.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175404.1 Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu33281
Clone ID Related Accession (Same CDS sequence)	XM_006502657.4 , XM_017319915.1 , XM_017319915.2 , XM_006502657.3 , XM_006502658.1 , XM_006502658.2 , XM_030253080.1
Accession Version	XM_017319915.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	zinc finger protein Rlf isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319915.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATCA AGCATTTGTT GAAGTCTAAC TGCATCCCCC AAGCTACTGC TTTATCAAAA 
CTGTGTGCAG AGTCTAAAGA ACTTGCCCAG GTGTCGTGTT TTCAGCAAGC CTATATCACA 
TGTTTATGTT CCATGCTTCC CAGCGAAGAG GCTATTCAGG AGATTGCAAA GGTGGACTGC 
AAGGACGTGT TAGACATCAT ATGTAATCTG GAATCTGAGG GTCAGGATAA CACAGCATTT 
GTTCTTTGTA CAACTTACCT TACCCAGCAG CTCCAAACGG CCAGTGTATA TTGTTCTTGG 
GAGCTGACTC TCTTTTGGAG TAAACTGCAA AGAAGAATTG ACCCATCTTT GGAGACATTT 
TTGGAGCGTT GTCGGCAGTT TGGTGTCATA GCTAAAACAC AACAGCATTT ATTTTGCCTG 
ATTAGAGTCA TACAGACTGA AGCACAAGAT GCTGGCATTG GGGTATCAAT TTTATTATGT 
GTCAGAGCTC TTCAACTCAG ATCAAGTGAA GATGAGGAAA TGAAAGCTTC GGTCTGTAAA 
ACAATTTCCT GTCTTTTACC AGAAGATTTG GAAGTCAGAC GAGCCTGTCA GCTTACAGAA 
TTCTTAATTG AACCCAGTTT GGATGGATTC AATATGCTGG AAGAACTGTA TTTGCAGCCA 
GATCAAAAAT TTGATGAAGA AAACGCACCA GTTCCAAATT CCCTGCGGTG TGAGCTTTTA 
CTAGCTTTAA AAGCCCATTG GCCCTTTGAT CCTGAATTTT GGGACTGGAA AACTTTGAAG 
CGACACTGCC ACCAACTCCT GGGACAAGAA GCCTCAGATT CCGATGATGA CCTAAGTGGC 
TATGAAATGT CTATTAATGA TACAGATGTC TTAGAGTCAT TTCTCAGTGA CTATGATGAT 
GGAAAAGAAG ATAAGCAATA TAGAAGAAGT CTAACAGATC AGAATAAGGA GAAAAGAGAC 
AAAAAGCCCA TTGGTTCCTC TGAGAGATAC CAGAGGTGGC TTCAGTATAA GTTTTTCTGT 
TTGTTGTGTA AGCGGGAATG CATAGAGGCC AGGATTCTCC ATCATTCCAA GATGCACATG 
GAAGATGGAA TATATACCTG TCCAGTTTGT ATTAAAAAAT TCAAGAGAAA AGAGCTATTT 
GTTCCTCACG TAATGGAACA TGTTAAAATG CCACCAAGCA GAAGCCACCG TTCTAGAAAG 
AAATTACTAT TGAAAAGCGC TCAAAGGGGT ATTTATCCCA AGAGTCCTAC TGGAAGTCTG 
GAACAAAATC CAGAGCAAGC CAGGGGAGAG TCTCATGAAT ATGTCACATT TAGCAAGTTA 
GAAGACCGCC GCCTGCAAGA CAGAGACTTG TACCCGTGTC CGGGTACAGA TTGTTCCCGT 
GTGTTCAAAC AGTTTAAATA TTTAAGCGTG CATCTTAAAG CTGAACACCA AAATAATGAC 
GAAAACGCCA AGCACTACTT GGATATGAAA AATAGGAGAG AGAAGTGTAC TTATTGCCGG 
CGGCATTTCA TGTCAGCTTT TCACCTCCGA GAACATGAGC AGGTGCATTG TGGTCCTCAG 
CCTTACATGT GTGTCTCTAT AGATTGCTAT GCAAGGTTTG GGTCAGTGAA TGAACTCCTT 
AACCACAAGC AGAAGCATGA TGACCTCCGT TACAAATGTG AGTTGAATGG CTGCAATATT 
GTGTTCAGTG ACTTGGGCCA GCTTTACCAC CATGAAGCAC AACACTTCAG GGATGCATCC 
TACACATGCA ATGTCCTTGG CTGTAAAAAG TTCTATTATT CTAAAATTGA ATACCAGAAC 
CACCTCTCCA TGCATAATGT TGAAAGTCCA GATGGAGAAT TAAAGAAATC AGTGAAACTT 
GAGGAACCTG GAGCAGGTGG GAAGCAAGAT TGTGTGGACC AGTCCCATCT ACTTGATGAA 
ACTGAGAAGT CCCATTCTCT TGAAGATCAC CATCTCTGTC CAGGGTCAGC TAGTGCTCAT 
ATAGACACCA CAGAGACCCT GAAGGACAAC AGCGACAGCA ATTCTAGTGA TCAGTTAAGT 
CACAGCTCTT CCACGTCAAT AACTGAGGAG TTAATTGACA CCCTAGACCA CTCTGAAACC 
ATGCAGGACC TGTTACTGTC TCATGAGAAA GTCTTTGTTC CCTCCAGTTT AAAAGACAAG 
TGCTCCAATG TGGCTGTTTG TTTTGATGGA ACTAAATTCA CCTGTGGTTT TGACGGCTGT 
GGCTCCACCT ACAAAAACGC AAGAGGGATG CAGAAACATC TGCGGAAGGT GCATCCGTAC 
CACTGCAAGC CAAGAAAAAT AAAGACAAAA GACCTCTTTA ACTGCCTGGA TGACAAACAT 
AATGAAGCTG ACAAGTTTGA TGCAGAACCT AAACCTAGCT CTGATACAAA TAGTGACTCC 
CCAGATGAAG GTCCAGATCA CAGTATCCAC ACAAAATGCA AACGAGAACA TCAAGGTTAT 
TCCCCTGAAC CTTCCATTTG TGCTTCTAAA AGGCCATGTA CAGAGGATAC AATGTTGGAA 
CTTCTGTTAC GTTTGAAACA TTTAAGCTTG AAGAACTCAA TAGCACATGG CTCTTTCTCA 
GGGTCATTGC AAGGGTGCCC ATCTAGTGGT GCTAAGTCTC TTCAGTCAGT CCCATCTTCC 
ATTTCAGACG TTAATCTTCA GAATCAGGAT GAAAATATGC CAAGTCAGTA CCTTGCACAG 
TTGGCGGCAA AGCCTTTCTT CTGTGAGCTT CAAGGATGCA AATATGAGTT TGTGACCAGA 
GAAGCTTTGT TAATGCATTA TCTTAAAAAG CATAACTATT CAAAAGAGAA GGTCCTTCAG 
TTAACCATGT TTCAGCACCG ATATTCCCCA TTCCGGTGTC ACATCTGCCA AAGGTCATTT 
ACAAGAAAAA CACATCTTAG GATTCATTAT AAAAACAAAC ATCAAATTGG CAGTGACAGG 
GCAACTCACA GACTGTTAGA CAGTGAAAAA TGTGATCATG AAGGGCCATG CTCAGTAGAC 
AGACTGAAAG GTGACTGCTC TACAGAACTT GGTCCCAACA GTAATTCAGA GACCACGCAA 
TGTCATTCTA AAAAAGATGA ATGCAGCTCA GAAACAGACT TGGAGTCCTC TTGTGAGGAA 
ACAGAAAGTA AGATCTCTGG TATCTCATCA CCAATAGGTA GCCATAGAGA AGAGGGAGAG 
GAGAAAGAGG GGAGAGGAAG TAGGCGGACT GTTGCTAAAG GAAATCTGTG CTATATTTTG 
AATAAGTACC ACAAACCATT CCATTGTATC CACAAAACTT GCAATTCTTC ATTTACCAAT 
CTAAAAGGCC TGATTCGCCA TTACAGAACT GTGCATCAGT ACAACAAAGA GCAGTTATGC 
TTAGAGAAGG ACAAAGCAAG AACCAAAAGG GAGCTTGTAA AATGTAAAAA GCTATTTGCT 
TGCAAATATA AAGACTGTAA CAAGCGCTTC CTGTGCTCCA AGGCCCTGGC TAAGCACTGT 
AGTGACTCTC ATAACCTAGA CCACATTGAA GAGTCTAAAG TGCTTTCTGA GACGGAGTCT 
GCAGCGAGGT TTTCTTGTAA CCAGCCTCAG TGCCCTGCTG TTTTTTATTC ATTCAGTAAG 
TTGAAACACC ACCTGTTGGA ACAGCATAAT ATTGAAGGAG AAATCCATTC GGATTATGAG 
ATCCATTGTA CTCTTAATGG CTGTGGCCAG ATTTTCAGCC ATCGAAGTAA TTACTTCCAG 
CATGTCTACT ACCGACATAA GGACTATTAT GACAACCTGT TCAGCAGCCA GAAAGTAGCA 
AATGAGCGGC TTCTAAGGAG TGAAAAGGTG TGTCAGACAA CTCAGGCACA AGGACTGACG 
CAGACGCAGG GGCAGACACA GGGACAGACG CAGGGACAGG AACAGCAGGC TGCCAAAAGG 
CCATTTAATA CTAAAGCTAA AAAATGTGGC TTACTCAAAG ATAAAAAAGC TCCAATTACC 
TTTAAAACAA GAGCAGAAGC GATCCATATG TGTGTTGAGC ATTCTGAGCA CACACAGTAC 
CCGTGCATGG TTCAGGGATG CTTGTCTGTG GTGAAGCTGG AAAGCAGCAT TGTGAGACAT 
TACAAACGTA CCCACCAGAT GAATAGTGCC TATTTAGAGC AGCAGTTGGA GAACCTTGTG 
GTTTGTGTTA AGTATGGTAC CAAAATTAAA GACGAGCCCC CTTCAGAAGT AGAGCCCTGT 
GTAAAGAAAG AGGAAAGTAC TAGCTGTGAA TCGGTGCACA CAGAGAATGG AGCCCCAGGA 
GACAGCAGTG TGCCCCTCCC AAACACTGAT TCTACTTGCC CAGCTGAACA AGATGTCGGT 
CAGAAAGGGT GTTCAGAAAG AAACCCAGTT TTTGACACAC ACAGTCTGCT CTACAGGGGA 
ACTTTGAAAT GCAACCATTC TTCAGAAACC ACTTCTTTGG AACAGTGTAA TATAGCTCAG 
TCTTCTCCCT GTAAAATAGA AAGCCCCATA CCTAATCCCA GTGGGACAGA AAGTGGTACT 
TATTTCACGG ACTTCCAGCT GCCATTACCA AGGATCAAAG AAGAACCTGG GCAGCATAGT 
TCAGGGCAAG AAAACACTGT AAAGAATGCA ACCCAGGTCC CAAAAGAGAA TATTAGAAAG 
CATTCTCAGC CCAGGTCATT TGATCTGAAG ACTTACAAAC CTATGGGATT TGAGTCTTCC 
TTTCTGAAAT TTATTCAGGA AAGTGAAGAG AAGGATGATG ATTTTGATGA CTGGGAGCCT 
TCAGAGCACT TAACATTGAA TAACTCCTCT CACCCCAGTA ATGACTTGAC AGGGAATGTT 
GTGGCAGATA CCATAGTGAA TGAGAGTGCC CCTCAAGTTG ACATACCTCA TTCTTCCAGT 
GACCCTCCAG TTTCAGAGAA CCTGACTGCA GTCCCACCAT TAGTAGTAGC TGAAGCAACA 
GCAGTTCCTT CCTTGGAAAA CCTGAGGGTT GTGCTGGACA AAGCATTAAC AGACTGTGGA 
GAACTTGCCT TAAAACAGCT TCATTACCTT CGGCCTGTGG TTGTCCTTGA AAGATCTAAG 
TTCTCTACAC CAATTTTAGA CTTGTTTCCA ACTAAGAAGA CAGATGAGCT TTGTGTAGGA 
AGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175404.1
CDS	1833..6941
Translation	MRIKHLLKSNCIPQATALSKLCAESKELAQVSCFQQAYITCLCSMLPSEEAIQEIAKVDCKDVLDIICNLESEGQDNTAFVLCTTYLTQQLQTASVYCSWELTLFWSKLQRRIDPSLETFLERCRQFGVIAKTQQHLFCLIRVIQTEAQDAGIGVSILLCVRALQLRSSEDEEMKASVCKTISCLLPEDLEVRRACQLTEFLIEPSLDGFNMLEELYLQPDQKFDEENAPVPNSLRCELLLALKAHWPFDPEFWDWKTLKRHCHQLLGQEASDSDDDLSGYEMSINDTDVLESFLSDYDDGKEDKQYRRSLTDQNKEKRDKKPIGSSERYQRWLQYKFFCLLCKRECIEARILHHSKMHMEDGIYTCPVCIKKFKRKELFVPHVMEHVKMPPSRSHRSRKKLLLKSAQRGIYPKSPTGSLEQNPEQARGESHEYVTFSKLEDRRLQDRDLYPCPGTDCSRVFKQFKYLSVHLKAEHQNNDENAKHYLDMKNRREKCTYCRRHFMSAFHLREHEQVHCGPQPYMCVSIDCYARFGSVNELLNHKQKHDDLRYKCELNGCNIVFSDLGQLYHHEAQHFRDASYTCNVLGCKKFYYSKIEYQNHLSMHNVESPDGELKKSVKLEEPGAGGKQDCVDQSHLLDETEKSHSLEDHHLCPGSASAHIDTTETLKDNSDSNSSDQLSHSSSTSITEELIDTLDHSETMQDLLLSHEKVFVPSSLKDKCSNVAVCFDGTKFTCGFDGCGSTYKNARGMQKHLRKVHPYHCKPRKIKTKDLFNCLDDKHNEADKFDAEPKPSSDTNSDSPDEGPDHSIHTKCKREHQGYSPEPSICASKRPCTEDTMLELLLRLKHLSLKNSIAHGSFSGSLQGCPSSGAKSLQSVPSSISDVNLQNQDENMPSQYLAQLAAKPFFCELQGCKYEFVTREALLMHYLKKHNYSKEKVLQLTMFQHRYSPFRCHICQRSFTRKTHLRIHYKNKHQIGSDRATHRLLDSEKCDHEGPCSVDRLKGDCSTELGPNSNSETTQCHSKKDECSSETDLESSCEETESKISGISSPIGSHREEGEEKEGRGSRRTVAKGNLCYILNKYHKPFHCIHKTCNSSFTNLKGLIRHYRTVHQYNKEQLCLEKDKARTKRELVKCKKLFACKYKDCNKRFLCSKALAKHCSDSHNLDHIEESKVLSETESAARFSCNQPQCPAVFYSFSKLKHHLLEQHNIEGEIHSDYEIHCTLNGCGQIFSHRSNYFQHVYYRHKDYYDNLFSSQKVANERLLRSEKVCQTTQAQGLTQTQGQTQGQTQGQEQQAAKRPFNTKAKKCGLLKDKKAPITFKTRAEAIHMCVEHSEHTQYPCMVQGCLSVVKLESSIVRHYKRTHQMNSAYLEQQLENLVVCVKYGTKIKDEPPSEVEPCVKKEESTSCESVHTENGAPGDSSVPLPNTDSTCPAEQDVGQKGCSERNPVFDTHSLLYRGTLKCNHSSETTSLEQCNIAQSSPCKIESPIPNPSGTESGTYFTDFQLPLPRIKEEPGQHSSGQENTVKNATQVPKENIRKHSQPRSFDLKTYKPMGFESSFLKFIQESEEKDDDFDDWEPSEHLTLNNSSHPSNDLTGNVVADTIVNESAPQVDIPHSSSDPPVSENLTAVPPLVVAEATAVPSLENLRVVLDKALTDCGELALKQLHYLRPVVVLERSKFSTPILDLFPTKKTDELCVGSS

Target ORF information:

RefSeq Version	XM_017319915.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319915.2

ORF Insert Sequence:

ATGAGAATCA AGCATTTGTT GAAGTCTAAC TGCATCCCCC AAGCTACTGC TTTATCAAAA 
CTGTGTGCAG AGTCTAAAGA ACTTGCCCAG GTGTCGTGTT TTCAGCAAGC CTATATCACA 
TGTTTATGTT CCATGCTTCC CAGCGAAGAG GCTATTCAGG AGATTGCAAA GGTGGACTGC 
AAGGACGTGT TAGACATCAT ATGTAATCTG GAATCTGAGG GTCAGGATAA CACAGCATTT 
GTTCTTTGTA CAACTTACCT TACCCAGCAG CTCCAAACGG CCAGTGTATA TTGTTCTTGG 
GAGCTGACTC TCTTTTGGAG TAAACTGCAA AGAAGAATTG ACCCATCTTT GGAGACATTT 
TTGGAGCGTT GTCGGCAGTT TGGTGTCATA GCTAAAACAC AACAGCATTT ATTTTGCCTG 
ATTAGAGTCA TACAGACTGA AGCACAAGAT GCTGGCATTG GGGTATCAAT TTTATTATGT 
GTCAGAGCTC TTCAACTCAG ATCAAGTGAA GATGAGGAAA TGAAAGCTTC GGTCTGTAAA 
ACAATTTCCT GTCTTTTACC AGAAGATTTG GAAGTCAGAC GAGCCTGTCA GCTTACAGAA 
TTCTTAATTG AACCCAGTTT GGATGGATTC AATATGCTGG AAGAACTGTA TTTGCAGCCA 
GATCAAAAAT TTGATGAAGA AAACGCACCA GTTCCAAATT CCCTGCGGTG TGAGCTTTTA 
CTAGCTTTAA AAGCCCATTG GCCCTTTGAT CCTGAATTTT GGGACTGGAA AACTTTGAAG 
CGACACTGCC ACCAACTCCT GGGACAAGAA GCCTCAGATT CCGATGATGA CCTAAGTGGC 
TATGAAATGT CTATTAATGA TACAGATGTC TTAGAGTCAT TTCTCAGTGA CTATGATGAT 
GGAAAAGAAG ATAAGCAATA TAGAAGAAGT CTAACAGATC AGAATAAGGA GAAAAGAGAC 
AAAAAGCCCA TTGGTTCCTC TGAGAGATAC CAGAGGTGGC TTCAGTATAA GTTTTTCTGT 
TTGTTGTGTA AGCGGGAATG CATAGAGGCC AGGATTCTCC ATCATTCCAA GATGCACATG 
GAAGATGGAA TATATACCTG TCCAGTTTGT ATTAAAAAAT TCAAGAGAAA AGAGCTATTT 
GTTCCTCACG TAATGGAACA TGTTAAAATG CCACCAAGCA GAAGCCACCG TTCTAGAAAG 
AAATTACTAT TGAAAAGCGC TCAAAGGGGT ATTTATCCCA AGAGTCCTAC TGGAAGTCTG 
GAACAAAATC CAGAGCAAGC CAGGGGAGAG TCTCATGAAT ATGTCACATT TAGCAAGTTA 
GAAGACCGCC GCCTGCAAGA CAGAGACTTG TACCCGTGTC CGGGTACAGA TTGTTCCCGT 
GTGTTCAAAC AGTTTAAATA TTTAAGCGTG CATCTTAAAG CTGAACACCA AAATAATGAC 
GAAAACGCCA AGCACTACTT GGATATGAAA AATAGGAGAG AGAAGTGTAC TTATTGCCGG 
CGGCATTTCA TGTCAGCTTT TCACCTCCGA GAACATGAGC AGGTGCATTG TGGTCCTCAG 
CCTTACATGT GTGTCTCTAT AGATTGCTAT GCAAGGTTTG GGTCAGTGAA TGAACTCCTT 
AACCACAAGC AGAAGCATGA TGACCTCCGT TACAAATGTG AGTTGAATGG CTGCAATATT 
GTGTTCAGTG ACTTGGGCCA GCTTTACCAC CATGAAGCAC AACACTTCAG GGATGCATCC 
TACACATGCA ATGTCCTTGG CTGTAAAAAG TTCTATTATT CTAAAATTGA ATACCAGAAC 
CACCTCTCCA TGCATAATGT TGAAAGTCCA GATGGAGAAT TAAAGAAATC AGTGAAACTT 
GAGGAACCTG GAGCAGGTGG GAAGCAAGAT TGTGTGGACC AGTCCCATCT ACTTGATGAA 
ACTGAGAAGT CCCATTCTCT TGAAGATCAC CATCTCTGTC CAGGGTCAGC TAGTGCTCAT 
ATAGACACCA CAGAGACCCT GAAGGACAAC AGCGACAGCA ATTCTAGTGA TCAGTTAAGT 
CACAGCTCTT CCACGTCAAT AACTGAGGAG TTAATTGACA CCCTAGACCA CTCTGAAACC 
ATGCAGGACC TGTTACTGTC TCATGAGAAA GTCTTTGTTC CCTCCAGTTT AAAAGACAAG 
TGCTCCAATG TGGCTGTTTG TTTTGATGGA ACTAAATTCA CCTGTGGTTT TGACGGCTGT 
GGCTCCACCT ACAAAAACGC AAGAGGGATG CAGAAACATC TGCGGAAGGT GCATCCGTAC 
CACTGCAAGC CAAGAAAAAT AAAGACAAAA GACCTCTTTA ACTGCCTGGA TGACAAACAT 
AATGAAGCTG ACAAGTTTGA TGCAGAACCT AAACCTAGCT CTGATACAAA TAGTGACTCC 
CCAGATGAAG GTCCAGATCA CAGTATCCAC ACAAAATGCA AACGAGAACA TCAAGGTTAT 
TCCCCTGAAC CTTCCATTTG TGCTTCTAAA AGGCCATGTA CAGAGGATAC AATGTTGGAA 
CTTCTGTTAC GTTTGAAACA TTTAAGCTTG AAGAACTCAA TAGCACATGG CTCTTTCTCA 
GGGTCATTGC AAGGGTGCCC ATCTAGTGGT GCTAAGTCTC TTCAGTCAGT CCCATCTTCC 
ATTTCAGACG TTAATCTTCA GAATCAGGAT GAAAATATGC CAAGTCAGTA CCTTGCACAG 
TTGGCGGCAA AGCCTTTCTT CTGTGAGCTT CAAGGATGCA AATATGAGTT TGTGACCAGA 
GAAGCTTTGT TAATGCATTA TCTTAAAAAG CATAACTATT CAAAAGAGAA GGTCCTTCAG 
TTAACCATGT TTCAGCACCG ATATTCCCCA TTCCGGTGTC ACATCTGCCA AAGGTCATTT 
ACAAGAAAAA CACATCTTAG GATTCATTAT AAAAACAAAC ATCAAATTGG CAGTGACAGG 
GCAACTCACA GACTGTTAGA CAGTGAAAAA TGTGATCATG AAGGGCCATG CTCAGTAGAC 
AGACTGAAAG GTGACTGCTC TACAGAACTT GGTCCCAACA GTAATTCAGA GACCACGCAA 
TGTCATTCTA AAAAAGATGA ATGCAGCTCA GAAACAGACT TGGAGTCCTC TTGTGAGGAA 
ACAGAAAGTA AGATCTCTGG TATCTCATCA CCAATAGGTA GCCATAGAGA AGAGGGAGAG 
GAGAAAGAGG GGAGAGGAAG TAGGCGGACT GTTGCTAAAG GAAATCTGTG CTATATTTTG 
AATAAGTACC ACAAACCATT CCATTGTATC CACAAAACTT GCAATTCTTC ATTTACCAAT 
CTAAAAGGCC TGATTCGCCA TTACAGAACT GTGCATCAGT ACAACAAAGA GCAGTTATGC 
TTAGAGAAGG ACAAAGCAAG AACCAAAAGG GAGCTTGTAA AATGTAAAAA GCTATTTGCT 
TGCAAATATA AAGACTGTAA CAAGCGCTTC CTGTGCTCCA AGGCCCTGGC TAAGCACTGT 
AGTGACTCTC ATAACCTAGA CCACATTGAA GAGTCTAAAG TGCTTTCTGA GACGGAGTCT 
GCAGCGAGGT TTTCTTGTAA CCAGCCTCAG TGCCCTGCTG TTTTTTATTC ATTCAGTAAG 
TTGAAACACC ACCTGTTGGA ACAGCATAAT ATTGAAGGAG AAATCCATTC GGATTATGAG 
ATCCATTGTA CTCTTAATGG CTGTGGCCAG ATTTTCAGCC ATCGAAGTAA TTACTTCCAG 
CATGTCTACT ACCGACATAA GGACTATTAT GACAACCTGT TCAGCAGCCA GAAAGTAGCA 
AATGAGCGGC TTCTAAGGAG TGAAAAGGTG TGTCAGACAA CTCAGGCACA AGGACTGACG 
CAGACGCAGG GGCAGACACA GGGACAGACG CAGGGACAGG AACAGCAGGC TGCCAAAAGG 
CCATTTAATA CTAAAGCTAA AAAATGTGGC TTACTCAAAG ATAAAAAAGC TCCAATTACC 
TTTAAAACAA GAGCAGAAGC GATCCATATG TGTGTTGAGC ATTCTGAGCA CACACAGTAC 
CCGTGCATGG TTCAGGGATG CTTGTCTGTG GTGAAGCTGG AAAGCAGCAT TGTGAGACAT 
TACAAACGTA CCCACCAGAT GAATAGTGCC TATTTAGAGC AGCAGTTGGA GAACCTTGTG 
GTTTGTGTTA AGTATGGTAC CAAAATTAAA GACGAGCCCC CTTCAGAAGT AGAGCCCTGT 
GTAAAGAAAG AGGAAAGTAC TAGCTGTGAA TCGGTGCACA CAGAGAATGG AGCCCCAGGA 
GACAGCAGTG TGCCCCTCCC AAACACTGAT TCTACTTGCC CAGCTGAACA AGATGTCGGT 
CAGAAAGGGT GTTCAGAAAG AAACCCAGTT TTTGACACAC ACAGTCTGCT CTACAGGGGA 
ACTTTGAAAT GCAACCATTC TTCAGAAACC ACTTCTTTGG AACAGTGTAA TATAGCTCAG 
TCTTCTCCCT GTAAAATAGA AAGCCCCATA CCTAATCCCA GTGGGACAGA AAGTGGTACT 
TATTTCACGG ACTTCCAGCT GCCATTACCA AGGATCAAAG AAGAACCTGG GCAGCATAGT 
TCAGGGCAAG AAAACACTGT AAAGAATGCA ACCCAGGTCC CAAAAGAGAA TATTAGAAAG 
CATTCTCAGC CCAGGTCATT TGATCTGAAG ACTTACAAAC CTATGGGATT TGAGTCTTCC 
TTTCTGAAAT TTATTCAGGA AAGTGAAGAG AAGGATGATG ATTTTGATGA CTGGGAGCCT 
TCAGAGCACT TAACATTGAA TAACTCCTCT CACCCCAGTA ATGACTTGAC AGGGAATGTT 
GTGGCAGATA CCATAGTGAA TGAGAGTGCC CCTCAAGTTG ACATACCTCA TTCTTCCAGT 
GACCCTCCAG TTTCAGAGAA CCTGACTGCA GTCCCACCAT TAGTAGTAGC TGAAGCAACA 
GCAGTTCCTT CCTTGGAAAA CCTGAGGGTT GTGCTGGACA AAGCATTAAC AGACTGTGGA 
GAACTTGCCT TAAAACAGCT TCATTACCTT CGGCCTGTGG TTGTCCTTGA AAGATCTAAG 
TTCTCTACAC CAATTTTAGA CTTGTTTCCA ACTAAGAAGA CAGATGAGCT TTGTGTAGGA 
AGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rlf ( XM_006502657.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006502720.1 Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X3, mRNA.

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CloneID	OMu33281
Clone ID Related Accession (Same CDS sequence)	XM_006502657.4 , XM_017319915.1 , XM_017319915.2 , XM_006502657.3 , XM_006502658.1 , XM_006502658.2 , XM_030253080.1
Accession Version	XM_006502657.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	zinc finger protein Rlf isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006502657.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATCA AGCATTTGTT GAAGTCTAAC TGCATCCCCC AAGCTACTGC TTTATCAAAA 
CTGTGTGCAG AGTCTAAAGA ACTTGCCCAG GTGTCGTGTT TTCAGCAAGC CTATATCACA 
TGTTTATGTT CCATGCTTCC CAGCGAAGAG GCTATTCAGG AGATTGCAAA GGTGGACTGC 
AAGGACGTGT TAGACATCAT ATGTAATCTG GAATCTGAGG GTCAGGATAA CACAGCATTT 
GTTCTTTGTA CAACTTACCT TACCCAGCAG CTCCAAACGG CCAGTGTATA TTGTTCTTGG 
GAGCTGACTC TCTTTTGGAG TAAACTGCAA AGAAGAATTG ACCCATCTTT GGAGACATTT 
TTGGAGCGTT GTCGGCAGTT TGGTGTCATA GCTAAAACAC AACAGCATTT ATTTTGCCTG 
ATTAGAGTCA TACAGACTGA AGCACAAGAT GCTGGCATTG GGGTATCAAT TTTATTATGT 
GTCAGAGCTC TTCAACTCAG ATCAAGTGAA GATGAGGAAA TGAAAGCTTC GGTCTGTAAA 
ACAATTTCCT GTCTTTTACC AGAAGATTTG GAAGTCAGAC GAGCCTGTCA GCTTACAGAA 
TTCTTAATTG AACCCAGTTT GGATGGATTC AATATGCTGG AAGAACTGTA TTTGCAGCCA 
GATCAAAAAT TTGATGAAGA AAACGCACCA GTTCCAAATT CCCTGCGGTG TGAGCTTTTA 
CTAGCTTTAA AAGCCCATTG GCCCTTTGAT CCTGAATTTT GGGACTGGAA AACTTTGAAG 
CGACACTGCC ACCAACTCCT GGGACAAGAA GCCTCAGATT CCGATGATGA CCTAAGTGGC 
TATGAAATGT CTATTAATGA TACAGATGTC TTAGAGTCAT TTCTCAGTGA CTATGATGAT 
GGAAAAGAAG ATAAGCAATA TAGAAGAAGT CTAACAGATC AGAATAAGGA GAAAAGAGAC 
AAAAAGCCCA TTGGTTCCTC TGAGAGATAC CAGAGGTGGC TTCAGTATAA GTTTTTCTGT 
TTGTTGTGTA AGCGGGAATG CATAGAGGCC AGGATTCTCC ATCATTCCAA GATGCACATG 
GAAGATGGAA TATATACCTG TCCAGTTTGT ATTAAAAAAT TCAAGAGAAA AGAGCTATTT 
GTTCCTCACG TAATGGAACA TGTTAAAATG CCACCAAGCA GAAGCCACCG TTCTAGAAAG 
AAATTACTAT TGAAAAGCGC TCAAAGGGGT ATTTATCCCA AGAGTCCTAC TGGAAGTCTG 
GAACAAAATC CAGAGCAAGC CAGGGGAGAG TCTCATGAAT ATGTCACATT TAGCAAGTTA 
GAAGACCGCC GCCTGCAAGA CAGAGACTTG TACCCGTGTC CGGGTACAGA TTGTTCCCGT 
GTGTTCAAAC AGTTTAAATA TTTAAGCGTG CATCTTAAAG CTGAACACCA AAATAATGAC 
GAAAACGCCA AGCACTACTT GGATATGAAA AATAGGAGAG AGAAGTGTAC TTATTGCCGG 
CGGCATTTCA TGTCAGCTTT TCACCTCCGA GAACATGAGC AGGTGCATTG TGGTCCTCAG 
CCTTACATGT GTGTCTCTAT AGATTGCTAT GCAAGGTTTG GGTCAGTGAA TGAACTCCTT 
AACCACAAGC AGAAGCATGA TGACCTCCGT TACAAATGTG AGTTGAATGG CTGCAATATT 
GTGTTCAGTG ACTTGGGCCA GCTTTACCAC CATGAAGCAC AACACTTCAG GGATGCATCC 
TACACATGCA ATGTCCTTGG CTGTAAAAAG TTCTATTATT CTAAAATTGA ATACCAGAAC 
CACCTCTCCA TGCATAATGT TGAAAGTCCA GATGGAGAAT TAAAGAAATC AGTGAAACTT 
GAGGAACCTG GAGCAGGTGG GAAGCAAGAT TGTGTGGACC AGTCCCATCT ACTTGATGAA 
ACTGAGAAGT CCCATTCTCT TGAAGATCAC CATCTCTGTC CAGGGTCAGC TAGTGCTCAT 
ATAGACACCA CAGAGACCCT GAAGGACAAC AGCGACAGCA ATTCTAGTGA TCAGTTAAGT 
CACAGCTCTT CCACGTCAAT AACTGAGGAG TTAATTGACA CCCTAGACCA CTCTGAAACC 
ATGCAGGACC TGTTACTGTC TCATGAGAAA GTCTTTGTTC CCTCCAGTTT AAAAGACAAG 
TGCTCCAATG TGGCTGTTTG TTTTGATGGA ACTAAATTCA CCTGTGGTTT TGACGGCTGT 
GGCTCCACCT ACAAAAACGC AAGAGGGATG CAGAAACATC TGCGGAAGGT GCATCCGTAC 
CACTGCAAGC CAAGAAAAAT AAAGACAAAA GACCTCTTTA ACTGCCTGGA TGACAAACAT 
AATGAAGCTG ACAAGTTTGA TGCAGAACCT AAACCTAGCT CTGATACAAA TAGTGACTCC 
CCAGATGAAG GTCCAGATCA CAGTATCCAC ACAAAATGCA AACGAGAACA TCAAGGTTAT 
TCCCCTGAAC CTTCCATTTG TGCTTCTAAA AGGCCATGTA CAGAGGATAC AATGTTGGAA 
CTTCTGTTAC GTTTGAAACA TTTAAGCTTG AAGAACTCAA TAGCACATGG CTCTTTCTCA 
GGGTCATTGC AAGGGTGCCC ATCTAGTGGT GCTAAGTCTC TTCAGTCAGT CCCATCTTCC 
ATTTCAGACG TTAATCTTCA GAATCAGGAT GAAAATATGC CAAGTCAGTA CCTTGCACAG 
TTGGCGGCAA AGCCTTTCTT CTGTGAGCTT CAAGGATGCA AATATGAGTT TGTGACCAGA 
GAAGCTTTGT TAATGCATTA TCTTAAAAAG CATAACTATT CAAAAGAGAA GGTCCTTCAG 
TTAACCATGT TTCAGCACCG ATATTCCCCA TTCCGGTGTC ACATCTGCCA AAGGTCATTT 
ACAAGAAAAA CACATCTTAG GATTCATTAT AAAAACAAAC ATCAAATTGG CAGTGACAGG 
GCAACTCACA GACTGTTAGA CAGTGAAAAA TGTGATCATG AAGGGCCATG CTCAGTAGAC 
AGACTGAAAG GTGACTGCTC TACAGAACTT GGTCCCAACA GTAATTCAGA GACCACGCAA 
TGTCATTCTA AAAAAGATGA ATGCAGCTCA GAAACAGACT TGGAGTCCTC TTGTGAGGAA 
ACAGAAAGTA AGATCTCTGG TATCTCATCA CCAATAGGTA GCCATAGAGA AGAGGGAGAG 
GAGAAAGAGG GGAGAGGAAG TAGGCGGACT GTTGCTAAAG GAAATCTGTG CTATATTTTG 
AATAAGTACC ACAAACCATT CCATTGTATC CACAAAACTT GCAATTCTTC ATTTACCAAT 
CTAAAAGGCC TGATTCGCCA TTACAGAACT GTGCATCAGT ACAACAAAGA GCAGTTATGC 
TTAGAGAAGG ACAAAGCAAG AACCAAAAGG GAGCTTGTAA AATGTAAAAA GCTATTTGCT 
TGCAAATATA AAGACTGTAA CAAGCGCTTC CTGTGCTCCA AGGCCCTGGC TAAGCACTGT 
AGTGACTCTC ATAACCTAGA CCACATTGAA GAGTCTAAAG TGCTTTCTGA GACGGAGTCT 
GCAGCGAGGT TTTCTTGTAA CCAGCCTCAG TGCCCTGCTG TTTTTTATTC ATTCAGTAAG 
TTGAAACACC ACCTGTTGGA ACAGCATAAT ATTGAAGGAG AAATCCATTC GGATTATGAG 
ATCCATTGTA CTCTTAATGG CTGTGGCCAG ATTTTCAGCC ATCGAAGTAA TTACTTCCAG 
CATGTCTACT ACCGACATAA GGACTATTAT GACAACCTGT TCAGCAGCCA GAAAGTAGCA 
AATGAGCGGC TTCTAAGGAG TGAAAAGGTG TGTCAGACAA CTCAGGCACA AGGACTGACG 
CAGACGCAGG GGCAGACACA GGGACAGACG CAGGGACAGG AACAGCAGGC TGCCAAAAGG 
CCATTTAATA CTAAAGCTAA AAAATGTGGC TTACTCAAAG ATAAAAAAGC TCCAATTACC 
TTTAAAACAA GAGCAGAAGC GATCCATATG TGTGTTGAGC ATTCTGAGCA CACACAGTAC 
CCGTGCATGG TTCAGGGATG CTTGTCTGTG GTGAAGCTGG AAAGCAGCAT TGTGAGACAT 
TACAAACGTA CCCACCAGAT GAATAGTGCC TATTTAGAGC AGCAGTTGGA GAACCTTGTG 
GTTTGTGTTA AGTATGGTAC CAAAATTAAA GACGAGCCCC CTTCAGAAGT AGAGCCCTGT 
GTAAAGAAAG AGGAAAGTAC TAGCTGTGAA TCGGTGCACA CAGAGAATGG AGCCCCAGGA 
GACAGCAGTG TGCCCCTCCC AAACACTGAT TCTACTTGCC CAGCTGAACA AGATGTCGGT 
CAGAAAGGGT GTTCAGAAAG AAACCCAGTT TTTGACACAC ACAGTCTGCT CTACAGGGGA 
ACTTTGAAAT GCAACCATTC TTCAGAAACC ACTTCTTTGG AACAGTGTAA TATAGCTCAG 
TCTTCTCCCT GTAAAATAGA AAGCCCCATA CCTAATCCCA GTGGGACAGA AAGTGGTACT 
TATTTCACGG ACTTCCAGCT GCCATTACCA AGGATCAAAG AAGAACCTGG GCAGCATAGT 
TCAGGGCAAG AAAACACTGT AAAGAATGCA ACCCAGGTCC CAAAAGAGAA TATTAGAAAG 
CATTCTCAGC CCAGGTCATT TGATCTGAAG ACTTACAAAC CTATGGGATT TGAGTCTTCC 
TTTCTGAAAT TTATTCAGGA AAGTGAAGAG AAGGATGATG ATTTTGATGA CTGGGAGCCT 
TCAGAGCACT TAACATTGAA TAACTCCTCT CACCCCAGTA ATGACTTGAC AGGGAATGTT 
GTGGCAGATA CCATAGTGAA TGAGAGTGCC CCTCAAGTTG ACATACCTCA TTCTTCCAGT 
GACCCTCCAG TTTCAGAGAA CCTGACTGCA GTCCCACCAT TAGTAGTAGC TGAAGCAACA 
GCAGTTCCTT CCTTGGAAAA CCTGAGGGTT GTGCTGGACA AAGCATTAAC AGACTGTGGA 
GAACTTGCCT TAAAACAGCT TCATTACCTT CGGCCTGTGG TTGTCCTTGA AAGATCTAAG 
TTCTCTACAC CAATTTTAGA CTTGTTTCCA ACTAAGAAGA CAGATGAGCT TTGTGTAGGA 
AGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006502720.1
CDS	204..5312
Translation	MRIKHLLKSNCIPQATALSKLCAESKELAQVSCFQQAYITCLCSMLPSEEAIQEIAKVDCKDVLDIICNLESEGQDNTAFVLCTTYLTQQLQTASVYCSWELTLFWSKLQRRIDPSLETFLERCRQFGVIAKTQQHLFCLIRVIQTEAQDAGIGVSILLCVRALQLRSSEDEEMKASVCKTISCLLPEDLEVRRACQLTEFLIEPSLDGFNMLEELYLQPDQKFDEENAPVPNSLRCELLLALKAHWPFDPEFWDWKTLKRHCHQLLGQEASDSDDDLSGYEMSINDTDVLESFLSDYDDGKEDKQYRRSLTDQNKEKRDKKPIGSSERYQRWLQYKFFCLLCKRECIEARILHHSKMHMEDGIYTCPVCIKKFKRKELFVPHVMEHVKMPPSRSHRSRKKLLLKSAQRGIYPKSPTGSLEQNPEQARGESHEYVTFSKLEDRRLQDRDLYPCPGTDCSRVFKQFKYLSVHLKAEHQNNDENAKHYLDMKNRREKCTYCRRHFMSAFHLREHEQVHCGPQPYMCVSIDCYARFGSVNELLNHKQKHDDLRYKCELNGCNIVFSDLGQLYHHEAQHFRDASYTCNVLGCKKFYYSKIEYQNHLSMHNVESPDGELKKSVKLEEPGAGGKQDCVDQSHLLDETEKSHSLEDHHLCPGSASAHIDTTETLKDNSDSNSSDQLSHSSSTSITEELIDTLDHSETMQDLLLSHEKVFVPSSLKDKCSNVAVCFDGTKFTCGFDGCGSTYKNARGMQKHLRKVHPYHCKPRKIKTKDLFNCLDDKHNEADKFDAEPKPSSDTNSDSPDEGPDHSIHTKCKREHQGYSPEPSICASKRPCTEDTMLELLLRLKHLSLKNSIAHGSFSGSLQGCPSSGAKSLQSVPSSISDVNLQNQDENMPSQYLAQLAAKPFFCELQGCKYEFVTREALLMHYLKKHNYSKEKVLQLTMFQHRYSPFRCHICQRSFTRKTHLRIHYKNKHQIGSDRATHRLLDSEKCDHEGPCSVDRLKGDCSTELGPNSNSETTQCHSKKDECSSETDLESSCEETESKISGISSPIGSHREEGEEKEGRGSRRTVAKGNLCYILNKYHKPFHCIHKTCNSSFTNLKGLIRHYRTVHQYNKEQLCLEKDKARTKRELVKCKKLFACKYKDCNKRFLCSKALAKHCSDSHNLDHIEESKVLSETESAARFSCNQPQCPAVFYSFSKLKHHLLEQHNIEGEIHSDYEIHCTLNGCGQIFSHRSNYFQHVYYRHKDYYDNLFSSQKVANERLLRSEKVCQTTQAQGLTQTQGQTQGQTQGQEQQAAKRPFNTKAKKCGLLKDKKAPITFKTRAEAIHMCVEHSEHTQYPCMVQGCLSVVKLESSIVRHYKRTHQMNSAYLEQQLENLVVCVKYGTKIKDEPPSEVEPCVKKEESTSCESVHTENGAPGDSSVPLPNTDSTCPAEQDVGQKGCSERNPVFDTHSLLYRGTLKCNHSSETTSLEQCNIAQSSPCKIESPIPNPSGTESGTYFTDFQLPLPRIKEEPGQHSSGQENTVKNATQVPKENIRKHSQPRSFDLKTYKPMGFESSFLKFIQESEEKDDDFDDWEPSEHLTLNNSSHPSNDLTGNVVADTIVNESAPQVDIPHSSSDPPVSENLTAVPPLVVAEATAVPSLENLRVVLDKALTDCGELALKQLHYLRPVVVLERSKFSTPILDLFPTKKTDELCVGSS

Target ORF information:

RefSeq Version	XM_006502657.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006502657.3

ORF Insert Sequence:

ATGAGAATCA AGCATTTGTT GAAGTCTAAC TGCATCCCCC AAGCTACTGC TTTATCAAAA 
CTGTGTGCAG AGTCTAAAGA ACTTGCCCAG GTGTCGTGTT TTCAGCAAGC CTATATCACA 
TGTTTATGTT CCATGCTTCC CAGCGAAGAG GCTATTCAGG AGATTGCAAA GGTGGACTGC 
AAGGACGTGT TAGACATCAT ATGTAATCTG GAATCTGAGG GTCAGGATAA CACAGCATTT 
GTTCTTTGTA CAACTTACCT TACCCAGCAG CTCCAAACGG CCAGTGTATA TTGTTCTTGG 
GAGCTGACTC TCTTTTGGAG TAAACTGCAA AGAAGAATTG ACCCATCTTT GGAGACATTT 
TTGGAGCGTT GTCGGCAGTT TGGTGTCATA GCTAAAACAC AACAGCATTT ATTTTGCCTG 
ATTAGAGTCA TACAGACTGA AGCACAAGAT GCTGGCATTG GGGTATCAAT TTTATTATGT 
GTCAGAGCTC TTCAACTCAG ATCAAGTGAA GATGAGGAAA TGAAAGCTTC GGTCTGTAAA 
ACAATTTCCT GTCTTTTACC AGAAGATTTG GAAGTCAGAC GAGCCTGTCA GCTTACAGAA 
TTCTTAATTG AACCCAGTTT GGATGGATTC AATATGCTGG AAGAACTGTA TTTGCAGCCA 
GATCAAAAAT TTGATGAAGA AAACGCACCA GTTCCAAATT CCCTGCGGTG TGAGCTTTTA 
CTAGCTTTAA AAGCCCATTG GCCCTTTGAT CCTGAATTTT GGGACTGGAA AACTTTGAAG 
CGACACTGCC ACCAACTCCT GGGACAAGAA GCCTCAGATT CCGATGATGA CCTAAGTGGC 
TATGAAATGT CTATTAATGA TACAGATGTC TTAGAGTCAT TTCTCAGTGA CTATGATGAT 
GGAAAAGAAG ATAAGCAATA TAGAAGAAGT CTAACAGATC AGAATAAGGA GAAAAGAGAC 
AAAAAGCCCA TTGGTTCCTC TGAGAGATAC CAGAGGTGGC TTCAGTATAA GTTTTTCTGT 
TTGTTGTGTA AGCGGGAATG CATAGAGGCC AGGATTCTCC ATCATTCCAA GATGCACATG 
GAAGATGGAA TATATACCTG TCCAGTTTGT ATTAAAAAAT TCAAGAGAAA AGAGCTATTT 
GTTCCTCACG TAATGGAACA TGTTAAAATG CCACCAAGCA GAAGCCACCG TTCTAGAAAG 
AAATTACTAT TGAAAAGCGC TCAAAGGGGT ATTTATCCCA AGAGTCCTAC TGGAAGTCTG 
GAACAAAATC CAGAGCAAGC CAGGGGAGAG TCTCATGAAT ATGTCACATT TAGCAAGTTA 
GAAGACCGCC GCCTGCAAGA CAGAGACTTG TACCCGTGTC CGGGTACAGA TTGTTCCCGT 
GTGTTCAAAC AGTTTAAATA TTTAAGCGTG CATCTTAAAG CTGAACACCA AAATAATGAC 
GAAAACGCCA AGCACTACTT GGATATGAAA AATAGGAGAG AGAAGTGTAC TTATTGCCGG 
CGGCATTTCA TGTCAGCTTT TCACCTCCGA GAACATGAGC AGGTGCATTG TGGTCCTCAG 
CCTTACATGT GTGTCTCTAT AGATTGCTAT GCAAGGTTTG GGTCAGTGAA TGAACTCCTT 
AACCACAAGC AGAAGCATGA TGACCTCCGT TACAAATGTG AGTTGAATGG CTGCAATATT 
GTGTTCAGTG ACTTGGGCCA GCTTTACCAC CATGAAGCAC AACACTTCAG GGATGCATCC 
TACACATGCA ATGTCCTTGG CTGTAAAAAG TTCTATTATT CTAAAATTGA ATACCAGAAC 
CACCTCTCCA TGCATAATGT TGAAAGTCCA GATGGAGAAT TAAAGAAATC AGTGAAACTT 
GAGGAACCTG GAGCAGGTGG GAAGCAAGAT TGTGTGGACC AGTCCCATCT ACTTGATGAA 
ACTGAGAAGT CCCATTCTCT TGAAGATCAC CATCTCTGTC CAGGGTCAGC TAGTGCTCAT 
ATAGACACCA CAGAGACCCT GAAGGACAAC AGCGACAGCA ATTCTAGTGA TCAGTTAAGT 
CACAGCTCTT CCACGTCAAT AACTGAGGAG TTAATTGACA CCCTAGACCA CTCTGAAACC 
ATGCAGGACC TGTTACTGTC TCATGAGAAA GTCTTTGTTC CCTCCAGTTT AAAAGACAAG 
TGCTCCAATG TGGCTGTTTG TTTTGATGGA ACTAAATTCA CCTGTGGTTT TGACGGCTGT 
GGCTCCACCT ACAAAAACGC AAGAGGGATG CAGAAACATC TGCGGAAGGT GCATCCGTAC 
CACTGCAAGC CAAGAAAAAT AAAGACAAAA GACCTCTTTA ACTGCCTGGA TGACAAACAT 
AATGAAGCTG ACAAGTTTGA TGCAGAACCT AAACCTAGCT CTGATACAAA TAGTGACTCC 
CCAGATGAAG GTCCAGATCA CAGTATCCAC ACAAAATGCA AACGAGAACA TCAAGGTTAT 
TCCCCTGAAC CTTCCATTTG TGCTTCTAAA AGGCCATGTA CAGAGGATAC AATGTTGGAA 
CTTCTGTTAC GTTTGAAACA TTTAAGCTTG AAGAACTCAA TAGCACATGG CTCTTTCTCA 
GGGTCATTGC AAGGGTGCCC ATCTAGTGGT GCTAAGTCTC TTCAGTCAGT CCCATCTTCC 
ATTTCAGACG TTAATCTTCA GAATCAGGAT GAAAATATGC CAAGTCAGTA CCTTGCACAG 
TTGGCGGCAA AGCCTTTCTT CTGTGAGCTT CAAGGATGCA AATATGAGTT TGTGACCAGA 
GAAGCTTTGT TAATGCATTA TCTTAAAAAG CATAACTATT CAAAAGAGAA GGTCCTTCAG 
TTAACCATGT TTCAGCACCG ATATTCCCCA TTCCGGTGTC ACATCTGCCA AAGGTCATTT 
ACAAGAAAAA CACATCTTAG GATTCATTAT AAAAACAAAC ATCAAATTGG CAGTGACAGG 
GCAACTCACA GACTGTTAGA CAGTGAAAAA TGTGATCATG AAGGGCCATG CTCAGTAGAC 
AGACTGAAAG GTGACTGCTC TACAGAACTT GGTCCCAACA GTAATTCAGA GACCACGCAA 
TGTCATTCTA AAAAAGATGA ATGCAGCTCA GAAACAGACT TGGAGTCCTC TTGTGAGGAA 
ACAGAAAGTA AGATCTCTGG TATCTCATCA CCAATAGGTA GCCATAGAGA AGAGGGAGAG 
GAGAAAGAGG GGAGAGGAAG TAGGCGGACT GTTGCTAAAG GAAATCTGTG CTATATTTTG 
AATAAGTACC ACAAACCATT CCATTGTATC CACAAAACTT GCAATTCTTC ATTTACCAAT 
CTAAAAGGCC TGATTCGCCA TTACAGAACT GTGCATCAGT ACAACAAAGA GCAGTTATGC 
TTAGAGAAGG ACAAAGCAAG AACCAAAAGG GAGCTTGTAA AATGTAAAAA GCTATTTGCT 
TGCAAATATA AAGACTGTAA CAAGCGCTTC CTGTGCTCCA AGGCCCTGGC TAAGCACTGT 
AGTGACTCTC ATAACCTAGA CCACATTGAA GAGTCTAAAG TGCTTTCTGA GACGGAGTCT 
GCAGCGAGGT TTTCTTGTAA CCAGCCTCAG TGCCCTGCTG TTTTTTATTC ATTCAGTAAG 
TTGAAACACC ACCTGTTGGA ACAGCATAAT ATTGAAGGAG AAATCCATTC GGATTATGAG 
ATCCATTGTA CTCTTAATGG CTGTGGCCAG ATTTTCAGCC ATCGAAGTAA TTACTTCCAG 
CATGTCTACT ACCGACATAA GGACTATTAT GACAACCTGT TCAGCAGCCA GAAAGTAGCA 
AATGAGCGGC TTCTAAGGAG TGAAAAGGTG TGTCAGACAA CTCAGGCACA AGGACTGACG 
CAGACGCAGG GGCAGACACA GGGACAGACG CAGGGACAGG AACAGCAGGC TGCCAAAAGG 
CCATTTAATA CTAAAGCTAA AAAATGTGGC TTACTCAAAG ATAAAAAAGC TCCAATTACC 
TTTAAAACAA GAGCAGAAGC GATCCATATG TGTGTTGAGC ATTCTGAGCA CACACAGTAC 
CCGTGCATGG TTCAGGGATG CTTGTCTGTG GTGAAGCTGG AAAGCAGCAT TGTGAGACAT 
TACAAACGTA CCCACCAGAT GAATAGTGCC TATTTAGAGC AGCAGTTGGA GAACCTTGTG 
GTTTGTGTTA AGTATGGTAC CAAAATTAAA GACGAGCCCC CTTCAGAAGT AGAGCCCTGT 
GTAAAGAAAG AGGAAAGTAC TAGCTGTGAA TCGGTGCACA CAGAGAATGG AGCCCCAGGA 
GACAGCAGTG TGCCCCTCCC AAACACTGAT TCTACTTGCC CAGCTGAACA AGATGTCGGT 
CAGAAAGGGT GTTCAGAAAG AAACCCAGTT TTTGACACAC ACAGTCTGCT CTACAGGGGA 
ACTTTGAAAT GCAACCATTC TTCAGAAACC ACTTCTTTGG AACAGTGTAA TATAGCTCAG 
TCTTCTCCCT GTAAAATAGA AAGCCCCATA CCTAATCCCA GTGGGACAGA AAGTGGTACT 
TATTTCACGG ACTTCCAGCT GCCATTACCA AGGATCAAAG AAGAACCTGG GCAGCATAGT 
TCAGGGCAAG AAAACACTGT AAAGAATGCA ACCCAGGTCC CAAAAGAGAA TATTAGAAAG 
CATTCTCAGC CCAGGTCATT TGATCTGAAG ACTTACAAAC CTATGGGATT TGAGTCTTCC 
TTTCTGAAAT TTATTCAGGA AAGTGAAGAG AAGGATGATG ATTTTGATGA CTGGGAGCCT 
TCAGAGCACT TAACATTGAA TAACTCCTCT CACCCCAGTA ATGACTTGAC AGGGAATGTT 
GTGGCAGATA CCATAGTGAA TGAGAGTGCC CCTCAAGTTG ACATACCTCA TTCTTCCAGT 
GACCCTCCAG TTTCAGAGAA CCTGACTGCA GTCCCACCAT TAGTAGTAGC TGAAGCAACA 
GCAGTTCCTT CCTTGGAAAA CCTGAGGGTT GTGCTGGACA AAGCATTAAC AGACTGTGGA 
GAACTTGCCT TAAAACAGCT TCATTACCTT CGGCCTGTGG TTGTCCTTGA AAGATCTAAG 
TTCTCTACAC CAATTTTAGA CTTGTTTCCA ACTAAGAAGA CAGATGAGCT TTGTGTAGGA 
AGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rlf ( XM_006502658.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006502721.1 Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X4, mRNA.

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CloneID	OMu33281
Clone ID Related Accession (Same CDS sequence)	XM_006502657.4 , XM_017319915.1 , XM_017319915.2 , XM_006502657.3 , XM_006502658.1 , XM_006502658.2 , XM_030253080.1
Accession Version	XM_006502658.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	zinc finger protein Rlf isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATCA AGCATTTGTT GAAGTCTAAC TGCATCCCCC AAGCTACTGC TTTATCAAAA 
CTGTGTGCAG AGTCTAAAGA ACTTGCCCAG GTGTCGTGTT TTCAGCAAGC CTATATCACA 
TGTTTATGTT CCATGCTTCC CAGCGAAGAG GCTATTCAGG AGATTGCAAA GGTGGACTGC 
AAGGACGTGT TAGACATCAT ATGTAATCTG GAATCTGAGG GTCAGGATAA CACAGCATTT 
GTTCTTTGTA CAACTTACCT TACCCAGCAG CTCCAAACGG CCAGTGTATA TTGTTCTTGG 
GAGCTGACTC TCTTTTGGAG TAAACTGCAA AGAAGAATTG ACCCATCTTT GGAGACATTT 
TTGGAGCGTT GTCGGCAGTT TGGTGTCATA GCTAAAACAC AACAGCATTT ATTTTGCCTG 
ATTAGAGTCA TACAGACTGA AGCACAAGAT GCTGGCATTG GGGTATCAAT TTTATTATGT 
GTCAGAGCTC TTCAACTCAG ATCAAGTGAA GATGAGGAAA TGAAAGCTTC GGTCTGTAAA 
ACAATTTCCT GTCTTTTACC AGAAGATTTG GAAGTCAGAC GAGCCTGTCA GCTTACAGAA 
TTCTTAATTG AACCCAGTTT GGATGGATTC AATATGCTGG AAGAACTGTA TTTGCAGCCA 
GATCAAAAAT TTGATGAAGA AAACGCACCA GTTCCAAATT CCCTGCGGTG TGAGCTTTTA 
CTAGCTTTAA AAGCCCATTG GCCCTTTGAT CCTGAATTTT GGGACTGGAA AACTTTGAAG 
CGACACTGCC ACCAACTCCT GGGACAAGAA GCCTCAGATT CCGATGATGA CCTAAGTGGC 
TATGAAATGT CTATTAATGA TACAGATGTC TTAGAGTCAT TTCTCAGTGA CTATGATGAT 
GGAAAAGAAG ATAAGCAATA TAGAAGAAGT CTAACAGATC AGAATAAGGA GAAAAGAGAC 
AAAAAGCCCA TTGGTTCCTC TGAGAGATAC CAGAGGTGGC TTCAGTATAA GTTTTTCTGT 
TTGTTGTGTA AGCGGGAATG CATAGAGGCC AGGATTCTCC ATCATTCCAA GATGCACATG 
GAAGATGGAA TATATACCTG TCCAGTTTGT ATTAAAAAAT TCAAGAGAAA AGAGCTATTT 
GTTCCTCACG TAATGGAACA TGTTAAAATG CCACCAAGCA GAAGCCACCG TTCTAGAAAG 
AAATTACTAT TGAAAAGCGC TCAAAGGGGT ATTTATCCCA AGAGTCCTAC TGGAAGTCTG 
GAACAAAATC CAGAGCAAGC CAGGGGAGAG TCTCATGAAT ATGTCACATT TAGCAAGTTA 
GAAGACCGCC GCCTGCAAGA CAGAGACTTG TACCCGTGTC CGGGTACAGA TTGTTCCCGT 
GTGTTCAAAC AGTTTAAATA TTTAAGCGTG CATCTTAAAG CTGAACACCA AAATAATGAC 
GAAAACGCCA AGCACTACTT GGATATGAAA AATAGGAGAG AGAAGTGTAC TTATTGCCGG 
CGGCATTTCA TGTCAGCTTT TCACCTCCGA GAACATGAGC AGGTGCATTG TGGTCCTCAG 
CCTTACATGT GTGTCTCTAT AGATTGCTAT GCAAGGTTTG GGTCAGTGAA TGAACTCCTT 
AACCACAAGC AGAAGCATGA TGACCTCCGT TACAAATGTG AGTTGAATGG CTGCAATATT 
GTGTTCAGTG ACTTGGGCCA GCTTTACCAC CATGAAGCAC AACACTTCAG GGATGCATCC 
TACACATGCA ATGTCCTTGG CTGTAAAAAG TTCTATTATT CTAAAATTGA ATACCAGAAC 
CACCTCTCCA TGCATAATGT TGAAAGTCCA GATGGAGAAT TAAAGAAATC AGTGAAACTT 
GAGGAACCTG GAGCAGGTGG GAAGCAAGAT TGTGTGGACC AGTCCCATCT ACTTGATGAA 
ACTGAGAAGT CCCATTCTCT TGAAGATCAC CATCTCTGTC CAGGGTCAGC TAGTGCTCAT 
ATAGACACCA CAGAGACCCT GAAGGACAAC AGCGACAGCA ATTCTAGTGA TCAGTTAAGT 
CACAGCTCTT CCACGTCAAT AACTGAGGAG TTAATTGACA CCCTAGACCA CTCTGAAACC 
ATGCAGGACC TGTTACTGTC TCATGAGAAA GTCTTTGTTC CCTCCAGTTT AAAAGACAAG 
TGCTCCAATG TGGCTGTTTG TTTTGATGGA ACTAAATTCA CCTGTGGTTT TGACGGCTGT 
GGCTCCACCT ACAAAAACGC AAGAGGGATG CAGAAACATC TGCGGAAGGT GCATCCGTAC 
CACTGCAAGC CAAGAAAAAT AAAGACAAAA GACCTCTTTA ACTGCCTGGA TGACAAACAT 
AATGAAGCTG ACAAGTTTGA TGCAGAACCT AAACCTAGCT CTGATACAAA TAGTGACTCC 
CCAGATGAAG GTCCAGATCA CAGTATCCAC ACAAAATGCA AACGAGAACA TCAAGGTTAT 
TCCCCTGAAC CTTCCATTTG TGCTTCTAAA AGGCCATGTA CAGAGGATAC AATGTTGGAA 
CTTCTGTTAC GTTTGAAACA TTTAAGCTTG AAGAACTCAA TAGCACATGG CTCTTTCTCA 
GGGTCATTGC AAGGGTGCCC ATCTAGTGGT GCTAAGTCTC TTCAGTCAGT CCCATCTTCC 
ATTTCAGACG TTAATCTTCA GAATCAGGAT GAAAATATGC CAAGTCAGTA CCTTGCACAG 
TTGGCGGCAA AGCCTTTCTT CTGTGAGCTT CAAGGATGCA AATATGAGTT TGTGACCAGA 
GAAGCTTTGT TAATGCATTA TCTTAAAAAG CATAACTATT CAAAAGAGAA GGTCCTTCAG 
TTAACCATGT TTCAGCACCG ATATTCCCCA TTCCGGTGTC ACATCTGCCA AAGGTCATTT 
ACAAGAAAAA CACATCTTAG GATTCATTAT AAAAACAAAC ATCAAATTGG CAGTGACAGG 
GCAACTCACA GACTGTTAGA CAGTGAAAAA TGTGATCATG AAGGGCCATG CTCAGTAGAC 
AGACTGAAAG GTGACTGCTC TACAGAACTT GGTCCCAACA GTAATTCAGA GACCACGCAA 
TGTCATTCTA AAAAAGATGA ATGCAGCTCA GAAACAGACT TGGAGTCCTC TTGTGAGGAA 
ACAGAAAGTA AGATCTCTGG TATCTCATCA CCAATAGGTA GCCATAGAGA AGAGGGAGAG 
GAGAAAGAGG GGAGAGGAAG TAGGCGGACT GTTGCTAAAG GAAATCTGTG CTATATTTTG 
AATAAGTACC ACAAACCATT CCATTGTATC CACAAAACTT GCAATTCTTC ATTTACCAAT 
CTAAAAGGCC TGATTCGCCA TTACAGAACT GTGCATCAGT ACAACAAAGA GCAGTTATGC 
TTAGAGAAGG ACAAAGCAAG AACCAAAAGG GAGCTTGTAA AATGTAAAAA GCTATTTGCT 
TGCAAATATA AAGACTGTAA CAAGCGCTTC CTGTGCTCCA AGGCCCTGGC TAAGCACTGT 
AGTGACTCTC ATAACCTAGA CCACATTGAA GAGTCTAAAG TGCTTTCTGA GACGGAGTCT 
GCAGCGAGGT TTTCTTGTAA CCAGCCTCAG TGCCCTGCTG TTTTTTATTC ATTCAGTAAG 
TTGAAACACC ACCTGTTGGA ACAGCATAAT ATTGAAGGAG AAATCCATTC GGATTATGAG 
ATCCATTGTA CTCTTAATGG CTGTGGCCAG ATTTTCAGCC ATCGAAGTAA TTACTTCCAG 
CATGTCTACT ACCGACATAA GGACTATTAT GACAACCTGT TCAGCAGCCA GAAAGTAGCA 
AATGAGCGGC TTCTAAGGAG TGAAAAGGTG TGTCAGACAA CTCAGGCACA AGGACTGACG 
CAGACGCAGG GGCAGACACA GGGACAGACG CAGGGACAGG AACAGCAGGC TGCCAAAAGG 
CCATTTAATA CTAAAGCTAA AAAATGTGGC TTACTCAAAG ATAAAAAAGC TCCAATTACC 
TTTAAAACAA GAGCAGAAGC GATCCATATG TGTGTTGAGC ATTCTGAGCA CACACAGTAC 
CCGTGCATGG TTCAGGGATG CTTGTCTGTG GTGAAGCTGG AAAGCAGCAT TGTGAGACAT 
TACAAACGTA CCCACCAGAT GAATAGTGCC TATTTAGAGC AGCAGTTGGA GAACCTTGTG 
GTTTGTGTTA AGTATGGTAC CAAAATTAAA GACGAGCCCC CTTCAGAAGT AGAGCCCTGT 
GTAAAGAAAG AGGAAAGTAC TAGCTGTGAA TCGGTGCACA CAGAGAATGG AGCCCCAGGA 
GACAGCAGTG TGCCCCTCCC AAACACTGAT TCTACTTGCC CAGCTGAACA AGATGTCGGT 
CAGAAAGGGT GTTCAGAAAG AAACCCAGTT TTTGACACAC ACAGTCTGCT CTACAGGGGA 
ACTTTGAAAT GCAACCATTC TTCAGAAACC ACTTCTTTGG AACAGTGTAA TATAGCTCAG 
TCTTCTCCCT GTAAAATAGA AAGCCCCATA CCTAATCCCA GTGGGACAGA AAGTGGTACT 
TATTTCACGG ACTTCCAGCT GCCATTACCA AGGATCAAAG AAGAACCTGG GCAGCATAGT 
TCAGGGCAAG AAAACACTGT AAAGAATGCA ACCCAGGTCC CAAAAGAGAA TATTAGAAAG 
CATTCTCAGC CCAGGTCATT TGATCTGAAG ACTTACAAAC CTATGGGATT TGAGTCTTCC 
TTTCTGAAAT TTATTCAGGA AAGTGAAGAG AAGGATGATG ATTTTGATGA CTGGGAGCCT 
TCAGAGCACT TAACATTGAA TAACTCCTCT CACCCCAGTA ATGACTTGAC AGGGAATGTT 
GTGGCAGATA CCATAGTGAA TGAGAGTGCC CCTCAAGTTG ACATACCTCA TTCTTCCAGT 
GACCCTCCAG TTTCAGAGAA CCTGACTGCA GTCCCACCAT TAGTAGTAGC TGAAGCAACA 
GCAGTTCCTT CCTTGGAAAA CCTGAGGGTT GTGCTGGACA AAGCATTAAC AGACTGTGGA 
GAACTTGCCT TAAAACAGCT TCATTACCTT CGGCCTGTGG TTGTCCTTGA AAGATCTAAG 
TTCTCTACAC CAATTTTAGA CTTGTTTCCA ACTAAGAAGA CAGATGAGCT TTGTGTAGGA 
AGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006502721.1
CDS	284..5392
Translation	MRIKHLLKSNCIPQATALSKLCAESKELAQVSCFQQAYITCLCSMLPSEEAIQEIAKVDCKDVLDIICNLESEGQDNTAFVLCTTYLTQQLQTASVYCSWELTLFWSKLQRRIDPSLETFLERCRQFGVIAKTQQHLFCLIRVIQTEAQDAGIGVSILLCVRALQLRSSEDEEMKASVCKTISCLLPEDLEVRRACQLTEFLIEPSLDGFNMLEELYLQPDQKFDEENAPVPNSLRCELLLALKAHWPFDPEFWDWKTLKRHCHQLLGQEASDSDDDLSGYEMSINDTDVLESFLSDYDDGKEDKQYRRSLTDQNKEKRDKKPIGSSERYQRWLQYKFFCLLCKRECIEARILHHSKMHMEDGIYTCPVCIKKFKRKELFVPHVMEHVKMPPSRSHRSRKKLLLKSAQRGIYPKSPTGSLEQNPEQARGESHEYVTFSKLEDRRLQDRDLYPCPGTDCSRVFKQFKYLSVHLKAEHQNNDENAKHYLDMKNRREKCTYCRRHFMSAFHLREHEQVHCGPQPYMCVSIDCYARFGSVNELLNHKQKHDDLRYKCELNGCNIVFSDLGQLYHHEAQHFRDASYTCNVLGCKKFYYSKIEYQNHLSMHNVESPDGELKKSVKLEEPGAGGKQDCVDQSHLLDETEKSHSLEDHHLCPGSASAHIDTTETLKDNSDSNSSDQLSHSSSTSITEELIDTLDHSETMQDLLLSHEKVFVPSSLKDKCSNVAVCFDGTKFTCGFDGCGSTYKNARGMQKHLRKVHPYHCKPRKIKTKDLFNCLDDKHNEADKFDAEPKPSSDTNSDSPDEGPDHSIHTKCKREHQGYSPEPSICASKRPCTEDTMLELLLRLKHLSLKNSIAHGSFSGSLQGCPSSGAKSLQSVPSSISDVNLQNQDENMPSQYLAQLAAKPFFCELQGCKYEFVTREALLMHYLKKHNYSKEKVLQLTMFQHRYSPFRCHICQRSFTRKTHLRIHYKNKHQIGSDRATHRLLDSEKCDHEGPCSVDRLKGDCSTELGPNSNSETTQCHSKKDECSSETDLESSCEETESKISGISSPIGSHREEGEEKEGRGSRRTVAKGNLCYILNKYHKPFHCIHKTCNSSFTNLKGLIRHYRTVHQYNKEQLCLEKDKARTKRELVKCKKLFACKYKDCNKRFLCSKALAKHCSDSHNLDHIEESKVLSETESAARFSCNQPQCPAVFYSFSKLKHHLLEQHNIEGEIHSDYEIHCTLNGCGQIFSHRSNYFQHVYYRHKDYYDNLFSSQKVANERLLRSEKVCQTTQAQGLTQTQGQTQGQTQGQEQQAAKRPFNTKAKKCGLLKDKKAPITFKTRAEAIHMCVEHSEHTQYPCMVQGCLSVVKLESSIVRHYKRTHQMNSAYLEQQLENLVVCVKYGTKIKDEPPSEVEPCVKKEESTSCESVHTENGAPGDSSVPLPNTDSTCPAEQDVGQKGCSERNPVFDTHSLLYRGTLKCNHSSETTSLEQCNIAQSSPCKIESPIPNPSGTESGTYFTDFQLPLPRIKEEPGQHSSGQENTVKNATQVPKENIRKHSQPRSFDLKTYKPMGFESSFLKFIQESEEKDDDFDDWEPSEHLTLNNSSHPSNDLTGNVVADTIVNESAPQVDIPHSSSDPPVSENLTAVPPLVVAEATAVPSLENLRVVLDKALTDCGELALKQLHYLRPVVVLERSKFSTPILDLFPTKKTDELCVGSS

Target ORF information:

RefSeq Version	XM_006502658.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006502658.1

ORF Insert Sequence:

ATGAGAATCA AGCATTTGTT GAAGTCTAAC TGCATCCCCC AAGCTACTGC TTTATCAAAA 
CTGTGTGCAG AGTCTAAAGA ACTTGCCCAG GTGTCGTGTT TTCAGCAAGC CTATATCACA 
TGTTTATGTT CCATGCTTCC CAGCGAAGAG GCTATTCAGG AGATTGCAAA GGTGGACTGC 
AAGGACGTGT TAGACATCAT ATGTAATCTG GAATCTGAGG GTCAGGATAA CACAGCATTT 
GTTCTTTGTA CAACTTACCT TACCCAGCAG CTCCAAACGG CCAGTGTATA TTGTTCTTGG 
GAGCTGACTC TCTTTTGGAG TAAACTGCAA AGAAGAATTG ACCCATCTTT GGAGACATTT 
TTGGAGCGTT GTCGGCAGTT TGGTGTCATA GCTAAAACAC AACAGCATTT ATTTTGCCTG 
ATTAGAGTCA TACAGACTGA AGCACAAGAT GCTGGCATTG GGGTATCAAT TTTATTATGT 
GTCAGAGCTC TTCAACTCAG ATCAAGTGAA GATGAGGAAA TGAAAGCTTC GGTCTGTAAA 
ACAATTTCCT GTCTTTTACC AGAAGATTTG GAAGTCAGAC GAGCCTGTCA GCTTACAGAA 
TTCTTAATTG AACCCAGTTT GGATGGATTC AATATGCTGG AAGAACTGTA TTTGCAGCCA 
GATCAAAAAT TTGATGAAGA AAACGCACCA GTTCCAAATT CCCTGCGGTG TGAGCTTTTA 
CTAGCTTTAA AAGCCCATTG GCCCTTTGAT CCTGAATTTT GGGACTGGAA AACTTTGAAG 
CGACACTGCC ACCAACTCCT GGGACAAGAA GCCTCAGATT CCGATGATGA CCTAAGTGGC 
TATGAAATGT CTATTAATGA TACAGATGTC TTAGAGTCAT TTCTCAGTGA CTATGATGAT 
GGAAAAGAAG ATAAGCAATA TAGAAGAAGT CTAACAGATC AGAATAAGGA GAAAAGAGAC 
AAAAAGCCCA TTGGTTCCTC TGAGAGATAC CAGAGGTGGC TTCAGTATAA GTTTTTCTGT 
TTGTTGTGTA AGCGGGAATG CATAGAGGCC AGGATTCTCC ATCATTCCAA GATGCACATG 
GAAGATGGAA TATATACCTG TCCAGTTTGT ATTAAAAAAT TCAAGAGAAA AGAGCTATTT 
GTTCCTCACG TAATGGAACA TGTTAAAATG CCACCAAGCA GAAGCCACCG TTCTAGAAAG 
AAATTACTAT TGAAAAGCGC TCAAAGGGGT ATTTATCCCA AGAGTCCTAC TGGAAGTCTG 
GAACAAAATC CAGAGCAAGC CAGGGGAGAG TCTCATGAAT ATGTCACATT TAGCAAGTTA 
GAAGACCGCC GCCTGCAAGA CAGAGACTTG TACCCGTGTC CGGGTACAGA TTGTTCCCGT 
GTGTTCAAAC AGTTTAAATA TTTAAGCGTG CATCTTAAAG CTGAACACCA AAATAATGAC 
GAAAACGCCA AGCACTACTT GGATATGAAA AATAGGAGAG AGAAGTGTAC TTATTGCCGG 
CGGCATTTCA TGTCAGCTTT TCACCTCCGA GAACATGAGC AGGTGCATTG TGGTCCTCAG 
CCTTACATGT GTGTCTCTAT AGATTGCTAT GCAAGGTTTG GGTCAGTGAA TGAACTCCTT 
AACCACAAGC AGAAGCATGA TGACCTCCGT TACAAATGTG AGTTGAATGG CTGCAATATT 
GTGTTCAGTG ACTTGGGCCA GCTTTACCAC CATGAAGCAC AACACTTCAG GGATGCATCC 
TACACATGCA ATGTCCTTGG CTGTAAAAAG TTCTATTATT CTAAAATTGA ATACCAGAAC 
CACCTCTCCA TGCATAATGT TGAAAGTCCA GATGGAGAAT TAAAGAAATC AGTGAAACTT 
GAGGAACCTG GAGCAGGTGG GAAGCAAGAT TGTGTGGACC AGTCCCATCT ACTTGATGAA 
ACTGAGAAGT CCCATTCTCT TGAAGATCAC CATCTCTGTC CAGGGTCAGC TAGTGCTCAT 
ATAGACACCA CAGAGACCCT GAAGGACAAC AGCGACAGCA ATTCTAGTGA TCAGTTAAGT 
CACAGCTCTT CCACGTCAAT AACTGAGGAG TTAATTGACA CCCTAGACCA CTCTGAAACC 
ATGCAGGACC TGTTACTGTC TCATGAGAAA GTCTTTGTTC CCTCCAGTTT AAAAGACAAG 
TGCTCCAATG TGGCTGTTTG TTTTGATGGA ACTAAATTCA CCTGTGGTTT TGACGGCTGT 
GGCTCCACCT ACAAAAACGC AAGAGGGATG CAGAAACATC TGCGGAAGGT GCATCCGTAC 
CACTGCAAGC CAAGAAAAAT AAAGACAAAA GACCTCTTTA ACTGCCTGGA TGACAAACAT 
AATGAAGCTG ACAAGTTTGA TGCAGAACCT AAACCTAGCT CTGATACAAA TAGTGACTCC 
CCAGATGAAG GTCCAGATCA CAGTATCCAC ACAAAATGCA AACGAGAACA TCAAGGTTAT 
TCCCCTGAAC CTTCCATTTG TGCTTCTAAA AGGCCATGTA CAGAGGATAC AATGTTGGAA 
CTTCTGTTAC GTTTGAAACA TTTAAGCTTG AAGAACTCAA TAGCACATGG CTCTTTCTCA 
GGGTCATTGC AAGGGTGCCC ATCTAGTGGT GCTAAGTCTC TTCAGTCAGT CCCATCTTCC 
ATTTCAGACG TTAATCTTCA GAATCAGGAT GAAAATATGC CAAGTCAGTA CCTTGCACAG 
TTGGCGGCAA AGCCTTTCTT CTGTGAGCTT CAAGGATGCA AATATGAGTT TGTGACCAGA 
GAAGCTTTGT TAATGCATTA TCTTAAAAAG CATAACTATT CAAAAGAGAA GGTCCTTCAG 
TTAACCATGT TTCAGCACCG ATATTCCCCA TTCCGGTGTC ACATCTGCCA AAGGTCATTT 
ACAAGAAAAA CACATCTTAG GATTCATTAT AAAAACAAAC ATCAAATTGG CAGTGACAGG 
GCAACTCACA GACTGTTAGA CAGTGAAAAA TGTGATCATG AAGGGCCATG CTCAGTAGAC 
AGACTGAAAG GTGACTGCTC TACAGAACTT GGTCCCAACA GTAATTCAGA GACCACGCAA 
TGTCATTCTA AAAAAGATGA ATGCAGCTCA GAAACAGACT TGGAGTCCTC TTGTGAGGAA 
ACAGAAAGTA AGATCTCTGG TATCTCATCA CCAATAGGTA GCCATAGAGA AGAGGGAGAG 
GAGAAAGAGG GGAGAGGAAG TAGGCGGACT GTTGCTAAAG GAAATCTGTG CTATATTTTG 
AATAAGTACC ACAAACCATT CCATTGTATC CACAAAACTT GCAATTCTTC ATTTACCAAT 
CTAAAAGGCC TGATTCGCCA TTACAGAACT GTGCATCAGT ACAACAAAGA GCAGTTATGC 
TTAGAGAAGG ACAAAGCAAG AACCAAAAGG GAGCTTGTAA AATGTAAAAA GCTATTTGCT 
TGCAAATATA AAGACTGTAA CAAGCGCTTC CTGTGCTCCA AGGCCCTGGC TAAGCACTGT 
AGTGACTCTC ATAACCTAGA CCACATTGAA GAGTCTAAAG TGCTTTCTGA GACGGAGTCT 
GCAGCGAGGT TTTCTTGTAA CCAGCCTCAG TGCCCTGCTG TTTTTTATTC ATTCAGTAAG 
TTGAAACACC ACCTGTTGGA ACAGCATAAT ATTGAAGGAG AAATCCATTC GGATTATGAG 
ATCCATTGTA CTCTTAATGG CTGTGGCCAG ATTTTCAGCC ATCGAAGTAA TTACTTCCAG 
CATGTCTACT ACCGACATAA GGACTATTAT GACAACCTGT TCAGCAGCCA GAAAGTAGCA 
AATGAGCGGC TTCTAAGGAG TGAAAAGGTG TGTCAGACAA CTCAGGCACA AGGACTGACG 
CAGACGCAGG GGCAGACACA GGGACAGACG CAGGGACAGG AACAGCAGGC TGCCAAAAGG 
CCATTTAATA CTAAAGCTAA AAAATGTGGC TTACTCAAAG ATAAAAAAGC TCCAATTACC 
TTTAAAACAA GAGCAGAAGC GATCCATATG TGTGTTGAGC ATTCTGAGCA CACACAGTAC 
CCGTGCATGG TTCAGGGATG CTTGTCTGTG GTGAAGCTGG AAAGCAGCAT TGTGAGACAT 
TACAAACGTA CCCACCAGAT GAATAGTGCC TATTTAGAGC AGCAGTTGGA GAACCTTGTG 
GTTTGTGTTA AGTATGGTAC CAAAATTAAA GACGAGCCCC CTTCAGAAGT AGAGCCCTGT 
GTAAAGAAAG AGGAAAGTAC TAGCTGTGAA TCGGTGCACA CAGAGAATGG AGCCCCAGGA 
GACAGCAGTG TGCCCCTCCC AAACACTGAT TCTACTTGCC CAGCTGAACA AGATGTCGGT 
CAGAAAGGGT GTTCAGAAAG AAACCCAGTT TTTGACACAC ACAGTCTGCT CTACAGGGGA 
ACTTTGAAAT GCAACCATTC TTCAGAAACC ACTTCTTTGG AACAGTGTAA TATAGCTCAG 
TCTTCTCCCT GTAAAATAGA AAGCCCCATA CCTAATCCCA GTGGGACAGA AAGTGGTACT 
TATTTCACGG ACTTCCAGCT GCCATTACCA AGGATCAAAG AAGAACCTGG GCAGCATAGT 
TCAGGGCAAG AAAACACTGT AAAGAATGCA ACCCAGGTCC CAAAAGAGAA TATTAGAAAG 
CATTCTCAGC CCAGGTCATT TGATCTGAAG ACTTACAAAC CTATGGGATT TGAGTCTTCC 
TTTCTGAAAT TTATTCAGGA AAGTGAAGAG AAGGATGATG ATTTTGATGA CTGGGAGCCT 
TCAGAGCACT TAACATTGAA TAACTCCTCT CACCCCAGTA ATGACTTGAC AGGGAATGTT 
GTGGCAGATA CCATAGTGAA TGAGAGTGCC CCTCAAGTTG ACATACCTCA TTCTTCCAGT 
GACCCTCCAG TTTCAGAGAA CCTGACTGCA GTCCCACCAT TAGTAGTAGC TGAAGCAACA 
GCAGTTCCTT CCTTGGAAAA CCTGAGGGTT GTGCTGGACA AAGCATTAAC AGACTGTGGA 
GAACTTGCCT TAAAACAGCT TCATTACCTT CGGCCTGTGG TTGTCCTTGA AAGATCTAAG 
TTCTCTACAC CAATTTTAGA CTTGTTTCCA ACTAAGAAGA CAGATGAGCT TTGTGTAGGA 
AGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rlf ( XM_006502658.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006502721.1 Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X4, mRNA.

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CloneID	OMu33281
Clone ID Related Accession (Same CDS sequence)	XM_006502657.4 , XM_017319915.1 , XM_017319915.2 , XM_006502657.3 , XM_006502658.1 , XM_006502658.2 , XM_030253080.1
Accession Version	XM_006502658.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	zinc finger protein Rlf isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006502658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATCA AGCATTTGTT GAAGTCTAAC TGCATCCCCC AAGCTACTGC TTTATCAAAA 
CTGTGTGCAG AGTCTAAAGA ACTTGCCCAG GTGTCGTGTT TTCAGCAAGC CTATATCACA 
TGTTTATGTT CCATGCTTCC CAGCGAAGAG GCTATTCAGG AGATTGCAAA GGTGGACTGC 
AAGGACGTGT TAGACATCAT ATGTAATCTG GAATCTGAGG GTCAGGATAA CACAGCATTT 
GTTCTTTGTA CAACTTACCT TACCCAGCAG CTCCAAACGG CCAGTGTATA TTGTTCTTGG 
GAGCTGACTC TCTTTTGGAG TAAACTGCAA AGAAGAATTG ACCCATCTTT GGAGACATTT 
TTGGAGCGTT GTCGGCAGTT TGGTGTCATA GCTAAAACAC AACAGCATTT ATTTTGCCTG 
ATTAGAGTCA TACAGACTGA AGCACAAGAT GCTGGCATTG GGGTATCAAT TTTATTATGT 
GTCAGAGCTC TTCAACTCAG ATCAAGTGAA GATGAGGAAA TGAAAGCTTC GGTCTGTAAA 
ACAATTTCCT GTCTTTTACC AGAAGATTTG GAAGTCAGAC GAGCCTGTCA GCTTACAGAA 
TTCTTAATTG AACCCAGTTT GGATGGATTC AATATGCTGG AAGAACTGTA TTTGCAGCCA 
GATCAAAAAT TTGATGAAGA AAACGCACCA GTTCCAAATT CCCTGCGGTG TGAGCTTTTA 
CTAGCTTTAA AAGCCCATTG GCCCTTTGAT CCTGAATTTT GGGACTGGAA AACTTTGAAG 
CGACACTGCC ACCAACTCCT GGGACAAGAA GCCTCAGATT CCGATGATGA CCTAAGTGGC 
TATGAAATGT CTATTAATGA TACAGATGTC TTAGAGTCAT TTCTCAGTGA CTATGATGAT 
GGAAAAGAAG ATAAGCAATA TAGAAGAAGT CTAACAGATC AGAATAAGGA GAAAAGAGAC 
AAAAAGCCCA TTGGTTCCTC TGAGAGATAC CAGAGGTGGC TTCAGTATAA GTTTTTCTGT 
TTGTTGTGTA AGCGGGAATG CATAGAGGCC AGGATTCTCC ATCATTCCAA GATGCACATG 
GAAGATGGAA TATATACCTG TCCAGTTTGT ATTAAAAAAT TCAAGAGAAA AGAGCTATTT 
GTTCCTCACG TAATGGAACA TGTTAAAATG CCACCAAGCA GAAGCCACCG TTCTAGAAAG 
AAATTACTAT TGAAAAGCGC TCAAAGGGGT ATTTATCCCA AGAGTCCTAC TGGAAGTCTG 
GAACAAAATC CAGAGCAAGC CAGGGGAGAG TCTCATGAAT ATGTCACATT TAGCAAGTTA 
GAAGACCGCC GCCTGCAAGA CAGAGACTTG TACCCGTGTC CGGGTACAGA TTGTTCCCGT 
GTGTTCAAAC AGTTTAAATA TTTAAGCGTG CATCTTAAAG CTGAACACCA AAATAATGAC 
GAAAACGCCA AGCACTACTT GGATATGAAA AATAGGAGAG AGAAGTGTAC TTATTGCCGG 
CGGCATTTCA TGTCAGCTTT TCACCTCCGA GAACATGAGC AGGTGCATTG TGGTCCTCAG 
CCTTACATGT GTGTCTCTAT AGATTGCTAT GCAAGGTTTG GGTCAGTGAA TGAACTCCTT 
AACCACAAGC AGAAGCATGA TGACCTCCGT TACAAATGTG AGTTGAATGG CTGCAATATT 
GTGTTCAGTG ACTTGGGCCA GCTTTACCAC CATGAAGCAC AACACTTCAG GGATGCATCC 
TACACATGCA ATGTCCTTGG CTGTAAAAAG TTCTATTATT CTAAAATTGA ATACCAGAAC 
CACCTCTCCA TGCATAATGT TGAAAGTCCA GATGGAGAAT TAAAGAAATC AGTGAAACTT 
GAGGAACCTG GAGCAGGTGG GAAGCAAGAT TGTGTGGACC AGTCCCATCT ACTTGATGAA 
ACTGAGAAGT CCCATTCTCT TGAAGATCAC CATCTCTGTC CAGGGTCAGC TAGTGCTCAT 
ATAGACACCA CAGAGACCCT GAAGGACAAC AGCGACAGCA ATTCTAGTGA TCAGTTAAGT 
CACAGCTCTT CCACGTCAAT AACTGAGGAG TTAATTGACA CCCTAGACCA CTCTGAAACC 
ATGCAGGACC TGTTACTGTC TCATGAGAAA GTCTTTGTTC CCTCCAGTTT AAAAGACAAG 
TGCTCCAATG TGGCTGTTTG TTTTGATGGA ACTAAATTCA CCTGTGGTTT TGACGGCTGT 
GGCTCCACCT ACAAAAACGC AAGAGGGATG CAGAAACATC TGCGGAAGGT GCATCCGTAC 
CACTGCAAGC CAAGAAAAAT AAAGACAAAA GACCTCTTTA ACTGCCTGGA TGACAAACAT 
AATGAAGCTG ACAAGTTTGA TGCAGAACCT AAACCTAGCT CTGATACAAA TAGTGACTCC 
CCAGATGAAG GTCCAGATCA CAGTATCCAC ACAAAATGCA AACGAGAACA TCAAGGTTAT 
TCCCCTGAAC CTTCCATTTG TGCTTCTAAA AGGCCATGTA CAGAGGATAC AATGTTGGAA 
CTTCTGTTAC GTTTGAAACA TTTAAGCTTG AAGAACTCAA TAGCACATGG CTCTTTCTCA 
GGGTCATTGC AAGGGTGCCC ATCTAGTGGT GCTAAGTCTC TTCAGTCAGT CCCATCTTCC 
ATTTCAGACG TTAATCTTCA GAATCAGGAT GAAAATATGC CAAGTCAGTA CCTTGCACAG 
TTGGCGGCAA AGCCTTTCTT CTGTGAGCTT CAAGGATGCA AATATGAGTT TGTGACCAGA 
GAAGCTTTGT TAATGCATTA TCTTAAAAAG CATAACTATT CAAAAGAGAA GGTCCTTCAG 
TTAACCATGT TTCAGCACCG ATATTCCCCA TTCCGGTGTC ACATCTGCCA AAGGTCATTT 
ACAAGAAAAA CACATCTTAG GATTCATTAT AAAAACAAAC ATCAAATTGG CAGTGACAGG 
GCAACTCACA GACTGTTAGA CAGTGAAAAA TGTGATCATG AAGGGCCATG CTCAGTAGAC 
AGACTGAAAG GTGACTGCTC TACAGAACTT GGTCCCAACA GTAATTCAGA GACCACGCAA 
TGTCATTCTA AAAAAGATGA ATGCAGCTCA GAAACAGACT TGGAGTCCTC TTGTGAGGAA 
ACAGAAAGTA AGATCTCTGG TATCTCATCA CCAATAGGTA GCCATAGAGA AGAGGGAGAG 
GAGAAAGAGG GGAGAGGAAG TAGGCGGACT GTTGCTAAAG GAAATCTGTG CTATATTTTG 
AATAAGTACC ACAAACCATT CCATTGTATC CACAAAACTT GCAATTCTTC ATTTACCAAT 
CTAAAAGGCC TGATTCGCCA TTACAGAACT GTGCATCAGT ACAACAAAGA GCAGTTATGC 
TTAGAGAAGG ACAAAGCAAG AACCAAAAGG GAGCTTGTAA AATGTAAAAA GCTATTTGCT 
TGCAAATATA AAGACTGTAA CAAGCGCTTC CTGTGCTCCA AGGCCCTGGC TAAGCACTGT 
AGTGACTCTC ATAACCTAGA CCACATTGAA GAGTCTAAAG TGCTTTCTGA GACGGAGTCT 
GCAGCGAGGT TTTCTTGTAA CCAGCCTCAG TGCCCTGCTG TTTTTTATTC ATTCAGTAAG 
TTGAAACACC ACCTGTTGGA ACAGCATAAT ATTGAAGGAG AAATCCATTC GGATTATGAG 
ATCCATTGTA CTCTTAATGG CTGTGGCCAG ATTTTCAGCC ATCGAAGTAA TTACTTCCAG 
CATGTCTACT ACCGACATAA GGACTATTAT GACAACCTGT TCAGCAGCCA GAAAGTAGCA 
AATGAGCGGC TTCTAAGGAG TGAAAAGGTG TGTCAGACAA CTCAGGCACA AGGACTGACG 
CAGACGCAGG GGCAGACACA GGGACAGACG CAGGGACAGG AACAGCAGGC TGCCAAAAGG 
CCATTTAATA CTAAAGCTAA AAAATGTGGC TTACTCAAAG ATAAAAAAGC TCCAATTACC 
TTTAAAACAA GAGCAGAAGC GATCCATATG TGTGTTGAGC ATTCTGAGCA CACACAGTAC 
CCGTGCATGG TTCAGGGATG CTTGTCTGTG GTGAAGCTGG AAAGCAGCAT TGTGAGACAT 
TACAAACGTA CCCACCAGAT GAATAGTGCC TATTTAGAGC AGCAGTTGGA GAACCTTGTG 
GTTTGTGTTA AGTATGGTAC CAAAATTAAA GACGAGCCCC CTTCAGAAGT AGAGCCCTGT 
GTAAAGAAAG AGGAAAGTAC TAGCTGTGAA TCGGTGCACA CAGAGAATGG AGCCCCAGGA 
GACAGCAGTG TGCCCCTCCC AAACACTGAT TCTACTTGCC CAGCTGAACA AGATGTCGGT 
CAGAAAGGGT GTTCAGAAAG AAACCCAGTT TTTGACACAC ACAGTCTGCT CTACAGGGGA 
ACTTTGAAAT GCAACCATTC TTCAGAAACC ACTTCTTTGG AACAGTGTAA TATAGCTCAG 
TCTTCTCCCT GTAAAATAGA AAGCCCCATA CCTAATCCCA GTGGGACAGA AAGTGGTACT 
TATTTCACGG ACTTCCAGCT GCCATTACCA AGGATCAAAG AAGAACCTGG GCAGCATAGT 
TCAGGGCAAG AAAACACTGT AAAGAATGCA ACCCAGGTCC CAAAAGAGAA TATTAGAAAG 
CATTCTCAGC CCAGGTCATT TGATCTGAAG ACTTACAAAC CTATGGGATT TGAGTCTTCC 
TTTCTGAAAT TTATTCAGGA AAGTGAAGAG AAGGATGATG ATTTTGATGA CTGGGAGCCT 
TCAGAGCACT TAACATTGAA TAACTCCTCT CACCCCAGTA ATGACTTGAC AGGGAATGTT 
GTGGCAGATA CCATAGTGAA TGAGAGTGCC CCTCAAGTTG ACATACCTCA TTCTTCCAGT 
GACCCTCCAG TTTCAGAGAA CCTGACTGCA GTCCCACCAT TAGTAGTAGC TGAAGCAACA 
GCAGTTCCTT CCTTGGAAAA CCTGAGGGTT GTGCTGGACA AAGCATTAAC AGACTGTGGA 
GAACTTGCCT TAAAACAGCT TCATTACCTT CGGCCTGTGG TTGTCCTTGA AAGATCTAAG 
TTCTCTACAC CAATTTTAGA CTTGTTTCCA ACTAAGAAGA CAGATGAGCT TTGTGTAGGA 
AGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006502721.1
CDS	317..5425
Translation	MRIKHLLKSNCIPQATALSKLCAESKELAQVSCFQQAYITCLCSMLPSEEAIQEIAKVDCKDVLDIICNLESEGQDNTAFVLCTTYLTQQLQTASVYCSWELTLFWSKLQRRIDPSLETFLERCRQFGVIAKTQQHLFCLIRVIQTEAQDAGIGVSILLCVRALQLRSSEDEEMKASVCKTISCLLPEDLEVRRACQLTEFLIEPSLDGFNMLEELYLQPDQKFDEENAPVPNSLRCELLLALKAHWPFDPEFWDWKTLKRHCHQLLGQEASDSDDDLSGYEMSINDTDVLESFLSDYDDGKEDKQYRRSLTDQNKEKRDKKPIGSSERYQRWLQYKFFCLLCKRECIEARILHHSKMHMEDGIYTCPVCIKKFKRKELFVPHVMEHVKMPPSRSHRSRKKLLLKSAQRGIYPKSPTGSLEQNPEQARGESHEYVTFSKLEDRRLQDRDLYPCPGTDCSRVFKQFKYLSVHLKAEHQNNDENAKHYLDMKNRREKCTYCRRHFMSAFHLREHEQVHCGPQPYMCVSIDCYARFGSVNELLNHKQKHDDLRYKCELNGCNIVFSDLGQLYHHEAQHFRDASYTCNVLGCKKFYYSKIEYQNHLSMHNVESPDGELKKSVKLEEPGAGGKQDCVDQSHLLDETEKSHSLEDHHLCPGSASAHIDTTETLKDNSDSNSSDQLSHSSSTSITEELIDTLDHSETMQDLLLSHEKVFVPSSLKDKCSNVAVCFDGTKFTCGFDGCGSTYKNARGMQKHLRKVHPYHCKPRKIKTKDLFNCLDDKHNEADKFDAEPKPSSDTNSDSPDEGPDHSIHTKCKREHQGYSPEPSICASKRPCTEDTMLELLLRLKHLSLKNSIAHGSFSGSLQGCPSSGAKSLQSVPSSISDVNLQNQDENMPSQYLAQLAAKPFFCELQGCKYEFVTREALLMHYLKKHNYSKEKVLQLTMFQHRYSPFRCHICQRSFTRKTHLRIHYKNKHQIGSDRATHRLLDSEKCDHEGPCSVDRLKGDCSTELGPNSNSETTQCHSKKDECSSETDLESSCEETESKISGISSPIGSHREEGEEKEGRGSRRTVAKGNLCYILNKYHKPFHCIHKTCNSSFTNLKGLIRHYRTVHQYNKEQLCLEKDKARTKRELVKCKKLFACKYKDCNKRFLCSKALAKHCSDSHNLDHIEESKVLSETESAARFSCNQPQCPAVFYSFSKLKHHLLEQHNIEGEIHSDYEIHCTLNGCGQIFSHRSNYFQHVYYRHKDYYDNLFSSQKVANERLLRSEKVCQTTQAQGLTQTQGQTQGQTQGQEQQAAKRPFNTKAKKCGLLKDKKAPITFKTRAEAIHMCVEHSEHTQYPCMVQGCLSVVKLESSIVRHYKRTHQMNSAYLEQQLENLVVCVKYGTKIKDEPPSEVEPCVKKEESTSCESVHTENGAPGDSSVPLPNTDSTCPAEQDVGQKGCSERNPVFDTHSLLYRGTLKCNHSSETTSLEQCNIAQSSPCKIESPIPNPSGTESGTYFTDFQLPLPRIKEEPGQHSSGQENTVKNATQVPKENIRKHSQPRSFDLKTYKPMGFESSFLKFIQESEEKDDDFDDWEPSEHLTLNNSSHPSNDLTGNVVADTIVNESAPQVDIPHSSSDPPVSENLTAVPPLVVAEATAVPSLENLRVVLDKALTDCGELALKQLHYLRPVVVLERSKFSTPILDLFPTKKTDELCVGSS

Target ORF information:

RefSeq Version	XM_006502658.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006502658.2

ORF Insert Sequence:

ATGAGAATCA AGCATTTGTT GAAGTCTAAC TGCATCCCCC AAGCTACTGC TTTATCAAAA 
CTGTGTGCAG AGTCTAAAGA ACTTGCCCAG GTGTCGTGTT TTCAGCAAGC CTATATCACA 
TGTTTATGTT CCATGCTTCC CAGCGAAGAG GCTATTCAGG AGATTGCAAA GGTGGACTGC 
AAGGACGTGT TAGACATCAT ATGTAATCTG GAATCTGAGG GTCAGGATAA CACAGCATTT 
GTTCTTTGTA CAACTTACCT TACCCAGCAG CTCCAAACGG CCAGTGTATA TTGTTCTTGG 
GAGCTGACTC TCTTTTGGAG TAAACTGCAA AGAAGAATTG ACCCATCTTT GGAGACATTT 
TTGGAGCGTT GTCGGCAGTT TGGTGTCATA GCTAAAACAC AACAGCATTT ATTTTGCCTG 
ATTAGAGTCA TACAGACTGA AGCACAAGAT GCTGGCATTG GGGTATCAAT TTTATTATGT 
GTCAGAGCTC TTCAACTCAG ATCAAGTGAA GATGAGGAAA TGAAAGCTTC GGTCTGTAAA 
ACAATTTCCT GTCTTTTACC AGAAGATTTG GAAGTCAGAC GAGCCTGTCA GCTTACAGAA 
TTCTTAATTG AACCCAGTTT GGATGGATTC AATATGCTGG AAGAACTGTA TTTGCAGCCA 
GATCAAAAAT TTGATGAAGA AAACGCACCA GTTCCAAATT CCCTGCGGTG TGAGCTTTTA 
CTAGCTTTAA AAGCCCATTG GCCCTTTGAT CCTGAATTTT GGGACTGGAA AACTTTGAAG 
CGACACTGCC ACCAACTCCT GGGACAAGAA GCCTCAGATT CCGATGATGA CCTAAGTGGC 
TATGAAATGT CTATTAATGA TACAGATGTC TTAGAGTCAT TTCTCAGTGA CTATGATGAT 
GGAAAAGAAG ATAAGCAATA TAGAAGAAGT CTAACAGATC AGAATAAGGA GAAAAGAGAC 
AAAAAGCCCA TTGGTTCCTC TGAGAGATAC CAGAGGTGGC TTCAGTATAA GTTTTTCTGT 
TTGTTGTGTA AGCGGGAATG CATAGAGGCC AGGATTCTCC ATCATTCCAA GATGCACATG 
GAAGATGGAA TATATACCTG TCCAGTTTGT ATTAAAAAAT TCAAGAGAAA AGAGCTATTT 
GTTCCTCACG TAATGGAACA TGTTAAAATG CCACCAAGCA GAAGCCACCG TTCTAGAAAG 
AAATTACTAT TGAAAAGCGC TCAAAGGGGT ATTTATCCCA AGAGTCCTAC TGGAAGTCTG 
GAACAAAATC CAGAGCAAGC CAGGGGAGAG TCTCATGAAT ATGTCACATT TAGCAAGTTA 
GAAGACCGCC GCCTGCAAGA CAGAGACTTG TACCCGTGTC CGGGTACAGA TTGTTCCCGT 
GTGTTCAAAC AGTTTAAATA TTTAAGCGTG CATCTTAAAG CTGAACACCA AAATAATGAC 
GAAAACGCCA AGCACTACTT GGATATGAAA AATAGGAGAG AGAAGTGTAC TTATTGCCGG 
CGGCATTTCA TGTCAGCTTT TCACCTCCGA GAACATGAGC AGGTGCATTG TGGTCCTCAG 
CCTTACATGT GTGTCTCTAT AGATTGCTAT GCAAGGTTTG GGTCAGTGAA TGAACTCCTT 
AACCACAAGC AGAAGCATGA TGACCTCCGT TACAAATGTG AGTTGAATGG CTGCAATATT 
GTGTTCAGTG ACTTGGGCCA GCTTTACCAC CATGAAGCAC AACACTTCAG GGATGCATCC 
TACACATGCA ATGTCCTTGG CTGTAAAAAG TTCTATTATT CTAAAATTGA ATACCAGAAC 
CACCTCTCCA TGCATAATGT TGAAAGTCCA GATGGAGAAT TAAAGAAATC AGTGAAACTT 
GAGGAACCTG GAGCAGGTGG GAAGCAAGAT TGTGTGGACC AGTCCCATCT ACTTGATGAA 
ACTGAGAAGT CCCATTCTCT TGAAGATCAC CATCTCTGTC CAGGGTCAGC TAGTGCTCAT 
ATAGACACCA CAGAGACCCT GAAGGACAAC AGCGACAGCA ATTCTAGTGA TCAGTTAAGT 
CACAGCTCTT CCACGTCAAT AACTGAGGAG TTAATTGACA CCCTAGACCA CTCTGAAACC 
ATGCAGGACC TGTTACTGTC TCATGAGAAA GTCTTTGTTC CCTCCAGTTT AAAAGACAAG 
TGCTCCAATG TGGCTGTTTG TTTTGATGGA ACTAAATTCA CCTGTGGTTT TGACGGCTGT 
GGCTCCACCT ACAAAAACGC AAGAGGGATG CAGAAACATC TGCGGAAGGT GCATCCGTAC 
CACTGCAAGC CAAGAAAAAT AAAGACAAAA GACCTCTTTA ACTGCCTGGA TGACAAACAT 
AATGAAGCTG ACAAGTTTGA TGCAGAACCT AAACCTAGCT CTGATACAAA TAGTGACTCC 
CCAGATGAAG GTCCAGATCA CAGTATCCAC ACAAAATGCA AACGAGAACA TCAAGGTTAT 
TCCCCTGAAC CTTCCATTTG TGCTTCTAAA AGGCCATGTA CAGAGGATAC AATGTTGGAA 
CTTCTGTTAC GTTTGAAACA TTTAAGCTTG AAGAACTCAA TAGCACATGG CTCTTTCTCA 
GGGTCATTGC AAGGGTGCCC ATCTAGTGGT GCTAAGTCTC TTCAGTCAGT CCCATCTTCC 
ATTTCAGACG TTAATCTTCA GAATCAGGAT GAAAATATGC CAAGTCAGTA CCTTGCACAG 
TTGGCGGCAA AGCCTTTCTT CTGTGAGCTT CAAGGATGCA AATATGAGTT TGTGACCAGA 
GAAGCTTTGT TAATGCATTA TCTTAAAAAG CATAACTATT CAAAAGAGAA GGTCCTTCAG 
TTAACCATGT TTCAGCACCG ATATTCCCCA TTCCGGTGTC ACATCTGCCA AAGGTCATTT 
ACAAGAAAAA CACATCTTAG GATTCATTAT AAAAACAAAC ATCAAATTGG CAGTGACAGG 
GCAACTCACA GACTGTTAGA CAGTGAAAAA TGTGATCATG AAGGGCCATG CTCAGTAGAC 
AGACTGAAAG GTGACTGCTC TACAGAACTT GGTCCCAACA GTAATTCAGA GACCACGCAA 
TGTCATTCTA AAAAAGATGA ATGCAGCTCA GAAACAGACT TGGAGTCCTC TTGTGAGGAA 
ACAGAAAGTA AGATCTCTGG TATCTCATCA CCAATAGGTA GCCATAGAGA AGAGGGAGAG 
GAGAAAGAGG GGAGAGGAAG TAGGCGGACT GTTGCTAAAG GAAATCTGTG CTATATTTTG 
AATAAGTACC ACAAACCATT CCATTGTATC CACAAAACTT GCAATTCTTC ATTTACCAAT 
CTAAAAGGCC TGATTCGCCA TTACAGAACT GTGCATCAGT ACAACAAAGA GCAGTTATGC 
TTAGAGAAGG ACAAAGCAAG AACCAAAAGG GAGCTTGTAA AATGTAAAAA GCTATTTGCT 
TGCAAATATA AAGACTGTAA CAAGCGCTTC CTGTGCTCCA AGGCCCTGGC TAAGCACTGT 
AGTGACTCTC ATAACCTAGA CCACATTGAA GAGTCTAAAG TGCTTTCTGA GACGGAGTCT 
GCAGCGAGGT TTTCTTGTAA CCAGCCTCAG TGCCCTGCTG TTTTTTATTC ATTCAGTAAG 
TTGAAACACC ACCTGTTGGA ACAGCATAAT ATTGAAGGAG AAATCCATTC GGATTATGAG 
ATCCATTGTA CTCTTAATGG CTGTGGCCAG ATTTTCAGCC ATCGAAGTAA TTACTTCCAG 
CATGTCTACT ACCGACATAA GGACTATTAT GACAACCTGT TCAGCAGCCA GAAAGTAGCA 
AATGAGCGGC TTCTAAGGAG TGAAAAGGTG TGTCAGACAA CTCAGGCACA AGGACTGACG 
CAGACGCAGG GGCAGACACA GGGACAGACG CAGGGACAGG AACAGCAGGC TGCCAAAAGG 
CCATTTAATA CTAAAGCTAA AAAATGTGGC TTACTCAAAG ATAAAAAAGC TCCAATTACC 
TTTAAAACAA GAGCAGAAGC GATCCATATG TGTGTTGAGC ATTCTGAGCA CACACAGTAC 
CCGTGCATGG TTCAGGGATG CTTGTCTGTG GTGAAGCTGG AAAGCAGCAT TGTGAGACAT 
TACAAACGTA CCCACCAGAT GAATAGTGCC TATTTAGAGC AGCAGTTGGA GAACCTTGTG 
GTTTGTGTTA AGTATGGTAC CAAAATTAAA GACGAGCCCC CTTCAGAAGT AGAGCCCTGT 
GTAAAGAAAG AGGAAAGTAC TAGCTGTGAA TCGGTGCACA CAGAGAATGG AGCCCCAGGA 
GACAGCAGTG TGCCCCTCCC AAACACTGAT TCTACTTGCC CAGCTGAACA AGATGTCGGT 
CAGAAAGGGT GTTCAGAAAG AAACCCAGTT TTTGACACAC ACAGTCTGCT CTACAGGGGA 
ACTTTGAAAT GCAACCATTC TTCAGAAACC ACTTCTTTGG AACAGTGTAA TATAGCTCAG 
TCTTCTCCCT GTAAAATAGA AAGCCCCATA CCTAATCCCA GTGGGACAGA AAGTGGTACT 
TATTTCACGG ACTTCCAGCT GCCATTACCA AGGATCAAAG AAGAACCTGG GCAGCATAGT 
TCAGGGCAAG AAAACACTGT AAAGAATGCA ACCCAGGTCC CAAAAGAGAA TATTAGAAAG 
CATTCTCAGC CCAGGTCATT TGATCTGAAG ACTTACAAAC CTATGGGATT TGAGTCTTCC 
TTTCTGAAAT TTATTCAGGA AAGTGAAGAG AAGGATGATG ATTTTGATGA CTGGGAGCCT 
TCAGAGCACT TAACATTGAA TAACTCCTCT CACCCCAGTA ATGACTTGAC AGGGAATGTT 
GTGGCAGATA CCATAGTGAA TGAGAGTGCC CCTCAAGTTG ACATACCTCA TTCTTCCAGT 
GACCCTCCAG TTTCAGAGAA CCTGACTGCA GTCCCACCAT TAGTAGTAGC TGAAGCAACA 
GCAGTTCCTT CCTTGGAAAA CCTGAGGGTT GTGCTGGACA AAGCATTAAC AGACTGTGGA 
GAACTTGCCT TAAAACAGCT TCATTACCTT CGGCCTGTGG TTGTCCTTGA AAGATCTAAG 
TTCTCTACAC CAATTTTAGA CTTGTTTCCA ACTAAGAAGA CAGATGAGCT TTGTGTAGGA 
AGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rlf ( XM_030253080.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108940.1 Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X2, mRNA.

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CloneID	OMu33281
Clone ID Related Accession (Same CDS sequence)	XM_006502657.4 , XM_017319915.1 , XM_017319915.2 , XM_006502657.3 , XM_006502658.1 , XM_006502658.2 , XM_030253080.1
Accession Version	XM_030253080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	zinc finger protein Rlf isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATCA AGCATTTGTT GAAGTCTAAC TGCATCCCCC AAGCTACTGC TTTATCAAAA 
CTGTGTGCAG AGTCTAAAGA ACTTGCCCAG GTGTCGTGTT TTCAGCAAGC CTATATCACA 
TGTTTATGTT CCATGCTTCC CAGCGAAGAG GCTATTCAGG AGATTGCAAA GGTGGACTGC 
AAGGACGTGT TAGACATCAT ATGTAATCTG GAATCTGAGG GTCAGGATAA CACAGCATTT 
GTTCTTTGTA CAACTTACCT TACCCAGCAG CTCCAAACGG CCAGTGTATA TTGTTCTTGG 
GAGCTGACTC TCTTTTGGAG TAAACTGCAA AGAAGAATTG ACCCATCTTT GGAGACATTT 
TTGGAGCGTT GTCGGCAGTT TGGTGTCATA GCTAAAACAC AACAGCATTT ATTTTGCCTG 
ATTAGAGTCA TACAGACTGA AGCACAAGAT GCTGGCATTG GGGTATCAAT TTTATTATGT 
GTCAGAGCTC TTCAACTCAG ATCAAGTGAA GATGAGGAAA TGAAAGCTTC GGTCTGTAAA 
ACAATTTCCT GTCTTTTACC AGAAGATTTG GAAGTCAGAC GAGCCTGTCA GCTTACAGAA 
TTCTTAATTG AACCCAGTTT GGATGGATTC AATATGCTGG AAGAACTGTA TTTGCAGCCA 
GATCAAAAAT TTGATGAAGA AAACGCACCA GTTCCAAATT CCCTGCGGTG TGAGCTTTTA 
CTAGCTTTAA AAGCCCATTG GCCCTTTGAT CCTGAATTTT GGGACTGGAA AACTTTGAAG 
CGACACTGCC ACCAACTCCT GGGACAAGAA GCCTCAGATT CCGATGATGA CCTAAGTGGC 
TATGAAATGT CTATTAATGA TACAGATGTC TTAGAGTCAT TTCTCAGTGA CTATGATGAT 
GGAAAAGAAG ATAAGCAATA TAGAAGAAGT CTAACAGATC AGAATAAGGA GAAAAGAGAC 
AAAAAGCCCA TTGGTTCCTC TGAGAGATAC CAGAGGTGGC TTCAGTATAA GTTTTTCTGT 
TTGTTGTGTA AGCGGGAATG CATAGAGGCC AGGATTCTCC ATCATTCCAA GATGCACATG 
GAAGATGGAA TATATACCTG TCCAGTTTGT ATTAAAAAAT TCAAGAGAAA AGAGCTATTT 
GTTCCTCACG TAATGGAACA TGTTAAAATG CCACCAAGCA GAAGCCACCG TTCTAGAAAG 
AAATTACTAT TGAAAAGCGC TCAAAGGGGT ATTTATCCCA AGAGTCCTAC TGGAAGTCTG 
GAACAAAATC CAGAGCAAGC CAGGGGAGAG TCTCATGAAT ATGTCACATT TAGCAAGTTA 
GAAGACCGCC GCCTGCAAGA CAGAGACTTG TACCCGTGTC CGGGTACAGA TTGTTCCCGT 
GTGTTCAAAC AGTTTAAATA TTTAAGCGTG CATCTTAAAG CTGAACACCA AAATAATGAC 
GAAAACGCCA AGCACTACTT GGATATGAAA AATAGGAGAG AGAAGTGTAC TTATTGCCGG 
CGGCATTTCA TGTCAGCTTT TCACCTCCGA GAACATGAGC AGGTGCATTG TGGTCCTCAG 
CCTTACATGT GTGTCTCTAT AGATTGCTAT GCAAGGTTTG GGTCAGTGAA TGAACTCCTT 
AACCACAAGC AGAAGCATGA TGACCTCCGT TACAAATGTG AGTTGAATGG CTGCAATATT 
GTGTTCAGTG ACTTGGGCCA GCTTTACCAC CATGAAGCAC AACACTTCAG GGATGCATCC 
TACACATGCA ATGTCCTTGG CTGTAAAAAG TTCTATTATT CTAAAATTGA ATACCAGAAC 
CACCTCTCCA TGCATAATGT TGAAAGTCCA GATGGAGAAT TAAAGAAATC AGTGAAACTT 
GAGGAACCTG GAGCAGGTGG GAAGCAAGAT TGTGTGGACC AGTCCCATCT ACTTGATGAA 
ACTGAGAAGT CCCATTCTCT TGAAGATCAC CATCTCTGTC CAGGGTCAGC TAGTGCTCAT 
ATAGACACCA CAGAGACCCT GAAGGACAAC AGCGACAGCA ATTCTAGTGA TCAGTTAAGT 
CACAGCTCTT CCACGTCAAT AACTGAGGAG TTAATTGACA CCCTAGACCA CTCTGAAACC 
ATGCAGGACC TGTTACTGTC TCATGAGAAA GTCTTTGTTC CCTCCAGTTT AAAAGACAAG 
TGCTCCAATG TGGCTGTTTG TTTTGATGGA ACTAAATTCA CCTGTGGTTT TGACGGCTGT 
GGCTCCACCT ACAAAAACGC AAGAGGGATG CAGAAACATC TGCGGAAGGT GCATCCGTAC 
CACTGCAAGC CAAGAAAAAT AAAGACAAAA GACCTCTTTA ACTGCCTGGA TGACAAACAT 
AATGAAGCTG ACAAGTTTGA TGCAGAACCT AAACCTAGCT CTGATACAAA TAGTGACTCC 
CCAGATGAAG GTCCAGATCA CAGTATCCAC ACAAAATGCA AACGAGAACA TCAAGGTTAT 
TCCCCTGAAC CTTCCATTTG TGCTTCTAAA AGGCCATGTA CAGAGGATAC AATGTTGGAA 
CTTCTGTTAC GTTTGAAACA TTTAAGCTTG AAGAACTCAA TAGCACATGG CTCTTTCTCA 
GGGTCATTGC AAGGGTGCCC ATCTAGTGGT GCTAAGTCTC TTCAGTCAGT CCCATCTTCC 
ATTTCAGACG TTAATCTTCA GAATCAGGAT GAAAATATGC CAAGTCAGTA CCTTGCACAG 
TTGGCGGCAA AGCCTTTCTT CTGTGAGCTT CAAGGATGCA AATATGAGTT TGTGACCAGA 
GAAGCTTTGT TAATGCATTA TCTTAAAAAG CATAACTATT CAAAAGAGAA GGTCCTTCAG 
TTAACCATGT TTCAGCACCG ATATTCCCCA TTCCGGTGTC ACATCTGCCA AAGGTCATTT 
ACAAGAAAAA CACATCTTAG GATTCATTAT AAAAACAAAC ATCAAATTGG CAGTGACAGG 
GCAACTCACA GACTGTTAGA CAGTGAAAAA TGTGATCATG AAGGGCCATG CTCAGTAGAC 
AGACTGAAAG GTGACTGCTC TACAGAACTT GGTCCCAACA GTAATTCAGA GACCACGCAA 
TGTCATTCTA AAAAAGATGA ATGCAGCTCA GAAACAGACT TGGAGTCCTC TTGTGAGGAA 
ACAGAAAGTA AGATCTCTGG TATCTCATCA CCAATAGGTA GCCATAGAGA AGAGGGAGAG 
GAGAAAGAGG GGAGAGGAAG TAGGCGGACT GTTGCTAAAG GAAATCTGTG CTATATTTTG 
AATAAGTACC ACAAACCATT CCATTGTATC CACAAAACTT GCAATTCTTC ATTTACCAAT 
CTAAAAGGCC TGATTCGCCA TTACAGAACT GTGCATCAGT ACAACAAAGA GCAGTTATGC 
TTAGAGAAGG ACAAAGCAAG AACCAAAAGG GAGCTTGTAA AATGTAAAAA GCTATTTGCT 
TGCAAATATA AAGACTGTAA CAAGCGCTTC CTGTGCTCCA AGGCCCTGGC TAAGCACTGT 
AGTGACTCTC ATAACCTAGA CCACATTGAA GAGTCTAAAG TGCTTTCTGA GACGGAGTCT 
GCAGCGAGGT TTTCTTGTAA CCAGCCTCAG TGCCCTGCTG TTTTTTATTC ATTCAGTAAG 
TTGAAACACC ACCTGTTGGA ACAGCATAAT ATTGAAGGAG AAATCCATTC GGATTATGAG 
ATCCATTGTA CTCTTAATGG CTGTGGCCAG ATTTTCAGCC ATCGAAGTAA TTACTTCCAG 
CATGTCTACT ACCGACATAA GGACTATTAT GACAACCTGT TCAGCAGCCA GAAAGTAGCA 
AATGAGCGGC TTCTAAGGAG TGAAAAGGTG TGTCAGACAA CTCAGGCACA AGGACTGACG 
CAGACGCAGG GGCAGACACA GGGACAGACG CAGGGACAGG AACAGCAGGC TGCCAAAAGG 
CCATTTAATA CTAAAGCTAA AAAATGTGGC TTACTCAAAG ATAAAAAAGC TCCAATTACC 
TTTAAAACAA GAGCAGAAGC GATCCATATG TGTGTTGAGC ATTCTGAGCA CACACAGTAC 
CCGTGCATGG TTCAGGGATG CTTGTCTGTG GTGAAGCTGG AAAGCAGCAT TGTGAGACAT 
TACAAACGTA CCCACCAGAT GAATAGTGCC TATTTAGAGC AGCAGTTGGA GAACCTTGTG 
GTTTGTGTTA AGTATGGTAC CAAAATTAAA GACGAGCCCC CTTCAGAAGT AGAGCCCTGT 
GTAAAGAAAG AGGAAAGTAC TAGCTGTGAA TCGGTGCACA CAGAGAATGG AGCCCCAGGA 
GACAGCAGTG TGCCCCTCCC AAACACTGAT TCTACTTGCC CAGCTGAACA AGATGTCGGT 
CAGAAAGGGT GTTCAGAAAG AAACCCAGTT TTTGACACAC ACAGTCTGCT CTACAGGGGA 
ACTTTGAAAT GCAACCATTC TTCAGAAACC ACTTCTTTGG AACAGTGTAA TATAGCTCAG 
TCTTCTCCCT GTAAAATAGA AAGCCCCATA CCTAATCCCA GTGGGACAGA AAGTGGTACT 
TATTTCACGG ACTTCCAGCT GCCATTACCA AGGATCAAAG AAGAACCTGG GCAGCATAGT 
TCAGGGCAAG AAAACACTGT AAAGAATGCA ACCCAGGTCC CAAAAGAGAA TATTAGAAAG 
CATTCTCAGC CCAGGTCATT TGATCTGAAG ACTTACAAAC CTATGGGATT TGAGTCTTCC 
TTTCTGAAAT TTATTCAGGA AAGTGAAGAG AAGGATGATG ATTTTGATGA CTGGGAGCCT 
TCAGAGCACT TAACATTGAA TAACTCCTCT CACCCCAGTA ATGACTTGAC AGGGAATGTT 
GTGGCAGATA CCATAGTGAA TGAGAGTGCC CCTCAAGTTG ACATACCTCA TTCTTCCAGT 
GACCCTCCAG TTTCAGAGAA CCTGACTGCA GTCCCACCAT TAGTAGTAGC TGAAGCAACA 
GCAGTTCCTT CCTTGGAAAA CCTGAGGGTT GTGCTGGACA AAGCATTAAC AGACTGTGGA 
GAACTTGCCT TAAAACAGCT TCATTACCTT CGGCCTGTGG TTGTCCTTGA AAGATCTAAG 
TTCTCTACAC CAATTTTAGA CTTGTTTCCA ACTAAGAAGA CAGATGAGCT TTGTGTAGGA 
AGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108940.1
CDS	752..5860
Translation	MRIKHLLKSNCIPQATALSKLCAESKELAQVSCFQQAYITCLCSMLPSEEAIQEIAKVDCKDVLDIICNLESEGQDNTAFVLCTTYLTQQLQTASVYCSWELTLFWSKLQRRIDPSLETFLERCRQFGVIAKTQQHLFCLIRVIQTEAQDAGIGVSILLCVRALQLRSSEDEEMKASVCKTISCLLPEDLEVRRACQLTEFLIEPSLDGFNMLEELYLQPDQKFDEENAPVPNSLRCELLLALKAHWPFDPEFWDWKTLKRHCHQLLGQEASDSDDDLSGYEMSINDTDVLESFLSDYDDGKEDKQYRRSLTDQNKEKRDKKPIGSSERYQRWLQYKFFCLLCKRECIEARILHHSKMHMEDGIYTCPVCIKKFKRKELFVPHVMEHVKMPPSRSHRSRKKLLLKSAQRGIYPKSPTGSLEQNPEQARGESHEYVTFSKLEDRRLQDRDLYPCPGTDCSRVFKQFKYLSVHLKAEHQNNDENAKHYLDMKNRREKCTYCRRHFMSAFHLREHEQVHCGPQPYMCVSIDCYARFGSVNELLNHKQKHDDLRYKCELNGCNIVFSDLGQLYHHEAQHFRDASYTCNVLGCKKFYYSKIEYQNHLSMHNVESPDGELKKSVKLEEPGAGGKQDCVDQSHLLDETEKSHSLEDHHLCPGSASAHIDTTETLKDNSDSNSSDQLSHSSSTSITEELIDTLDHSETMQDLLLSHEKVFVPSSLKDKCSNVAVCFDGTKFTCGFDGCGSTYKNARGMQKHLRKVHPYHCKPRKIKTKDLFNCLDDKHNEADKFDAEPKPSSDTNSDSPDEGPDHSIHTKCKREHQGYSPEPSICASKRPCTEDTMLELLLRLKHLSLKNSIAHGSFSGSLQGCPSSGAKSLQSVPSSISDVNLQNQDENMPSQYLAQLAAKPFFCELQGCKYEFVTREALLMHYLKKHNYSKEKVLQLTMFQHRYSPFRCHICQRSFTRKTHLRIHYKNKHQIGSDRATHRLLDSEKCDHEGPCSVDRLKGDCSTELGPNSNSETTQCHSKKDECSSETDLESSCEETESKISGISSPIGSHREEGEEKEGRGSRRTVAKGNLCYILNKYHKPFHCIHKTCNSSFTNLKGLIRHYRTVHQYNKEQLCLEKDKARTKRELVKCKKLFACKYKDCNKRFLCSKALAKHCSDSHNLDHIEESKVLSETESAARFSCNQPQCPAVFYSFSKLKHHLLEQHNIEGEIHSDYEIHCTLNGCGQIFSHRSNYFQHVYYRHKDYYDNLFSSQKVANERLLRSEKVCQTTQAQGLTQTQGQTQGQTQGQEQQAAKRPFNTKAKKCGLLKDKKAPITFKTRAEAIHMCVEHSEHTQYPCMVQGCLSVVKLESSIVRHYKRTHQMNSAYLEQQLENLVVCVKYGTKIKDEPPSEVEPCVKKEESTSCESVHTENGAPGDSSVPLPNTDSTCPAEQDVGQKGCSERNPVFDTHSLLYRGTLKCNHSSETTSLEQCNIAQSSPCKIESPIPNPSGTESGTYFTDFQLPLPRIKEEPGQHSSGQENTVKNATQVPKENIRKHSQPRSFDLKTYKPMGFESSFLKFIQESEEKDDDFDDWEPSEHLTLNNSSHPSNDLTGNVVADTIVNESAPQVDIPHSSSDPPVSENLTAVPPLVVAEATAVPSLENLRVVLDKALTDCGELALKQLHYLRPVVVLERSKFSTPILDLFPTKKTDELCVGSS

Target ORF information:

RefSeq Version	XM_030253080.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus rearranged L-myc fusion sequence (Rlf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253080.1

ORF Insert Sequence:

ATGAGAATCA AGCATTTGTT GAAGTCTAAC TGCATCCCCC AAGCTACTGC TTTATCAAAA 
CTGTGTGCAG AGTCTAAAGA ACTTGCCCAG GTGTCGTGTT TTCAGCAAGC CTATATCACA 
TGTTTATGTT CCATGCTTCC CAGCGAAGAG GCTATTCAGG AGATTGCAAA GGTGGACTGC 
AAGGACGTGT TAGACATCAT ATGTAATCTG GAATCTGAGG GTCAGGATAA CACAGCATTT 
GTTCTTTGTA CAACTTACCT TACCCAGCAG CTCCAAACGG CCAGTGTATA TTGTTCTTGG 
GAGCTGACTC TCTTTTGGAG TAAACTGCAA AGAAGAATTG ACCCATCTTT GGAGACATTT 
TTGGAGCGTT GTCGGCAGTT TGGTGTCATA GCTAAAACAC AACAGCATTT ATTTTGCCTG 
ATTAGAGTCA TACAGACTGA AGCACAAGAT GCTGGCATTG GGGTATCAAT TTTATTATGT 
GTCAGAGCTC TTCAACTCAG ATCAAGTGAA GATGAGGAAA TGAAAGCTTC GGTCTGTAAA 
ACAATTTCCT GTCTTTTACC AGAAGATTTG GAAGTCAGAC GAGCCTGTCA GCTTACAGAA 
TTCTTAATTG AACCCAGTTT GGATGGATTC AATATGCTGG AAGAACTGTA TTTGCAGCCA 
GATCAAAAAT TTGATGAAGA AAACGCACCA GTTCCAAATT CCCTGCGGTG TGAGCTTTTA 
CTAGCTTTAA AAGCCCATTG GCCCTTTGAT CCTGAATTTT GGGACTGGAA AACTTTGAAG 
CGACACTGCC ACCAACTCCT GGGACAAGAA GCCTCAGATT CCGATGATGA CCTAAGTGGC 
TATGAAATGT CTATTAATGA TACAGATGTC TTAGAGTCAT TTCTCAGTGA CTATGATGAT 
GGAAAAGAAG ATAAGCAATA TAGAAGAAGT CTAACAGATC AGAATAAGGA GAAAAGAGAC 
AAAAAGCCCA TTGGTTCCTC TGAGAGATAC CAGAGGTGGC TTCAGTATAA GTTTTTCTGT 
TTGTTGTGTA AGCGGGAATG CATAGAGGCC AGGATTCTCC ATCATTCCAA GATGCACATG 
GAAGATGGAA TATATACCTG TCCAGTTTGT ATTAAAAAAT TCAAGAGAAA AGAGCTATTT 
GTTCCTCACG TAATGGAACA TGTTAAAATG CCACCAAGCA GAAGCCACCG TTCTAGAAAG 
AAATTACTAT TGAAAAGCGC TCAAAGGGGT ATTTATCCCA AGAGTCCTAC TGGAAGTCTG 
GAACAAAATC CAGAGCAAGC CAGGGGAGAG TCTCATGAAT ATGTCACATT TAGCAAGTTA 
GAAGACCGCC GCCTGCAAGA CAGAGACTTG TACCCGTGTC CGGGTACAGA TTGTTCCCGT 
GTGTTCAAAC AGTTTAAATA TTTAAGCGTG CATCTTAAAG CTGAACACCA AAATAATGAC 
GAAAACGCCA AGCACTACTT GGATATGAAA AATAGGAGAG AGAAGTGTAC TTATTGCCGG 
CGGCATTTCA TGTCAGCTTT TCACCTCCGA GAACATGAGC AGGTGCATTG TGGTCCTCAG 
CCTTACATGT GTGTCTCTAT AGATTGCTAT GCAAGGTTTG GGTCAGTGAA TGAACTCCTT 
AACCACAAGC AGAAGCATGA TGACCTCCGT TACAAATGTG AGTTGAATGG CTGCAATATT 
GTGTTCAGTG ACTTGGGCCA GCTTTACCAC CATGAAGCAC AACACTTCAG GGATGCATCC 
TACACATGCA ATGTCCTTGG CTGTAAAAAG TTCTATTATT CTAAAATTGA ATACCAGAAC 
CACCTCTCCA TGCATAATGT TGAAAGTCCA GATGGAGAAT TAAAGAAATC AGTGAAACTT 
GAGGAACCTG GAGCAGGTGG GAAGCAAGAT TGTGTGGACC AGTCCCATCT ACTTGATGAA 
ACTGAGAAGT CCCATTCTCT TGAAGATCAC CATCTCTGTC CAGGGTCAGC TAGTGCTCAT 
ATAGACACCA CAGAGACCCT GAAGGACAAC AGCGACAGCA ATTCTAGTGA TCAGTTAAGT 
CACAGCTCTT CCACGTCAAT AACTGAGGAG TTAATTGACA CCCTAGACCA CTCTGAAACC 
ATGCAGGACC TGTTACTGTC TCATGAGAAA GTCTTTGTTC CCTCCAGTTT AAAAGACAAG 
TGCTCCAATG TGGCTGTTTG TTTTGATGGA ACTAAATTCA CCTGTGGTTT TGACGGCTGT 
GGCTCCACCT ACAAAAACGC AAGAGGGATG CAGAAACATC TGCGGAAGGT GCATCCGTAC 
CACTGCAAGC CAAGAAAAAT AAAGACAAAA GACCTCTTTA ACTGCCTGGA TGACAAACAT 
AATGAAGCTG ACAAGTTTGA TGCAGAACCT AAACCTAGCT CTGATACAAA TAGTGACTCC 
CCAGATGAAG GTCCAGATCA CAGTATCCAC ACAAAATGCA AACGAGAACA TCAAGGTTAT 
TCCCCTGAAC CTTCCATTTG TGCTTCTAAA AGGCCATGTA CAGAGGATAC AATGTTGGAA 
CTTCTGTTAC GTTTGAAACA TTTAAGCTTG AAGAACTCAA TAGCACATGG CTCTTTCTCA 
GGGTCATTGC AAGGGTGCCC ATCTAGTGGT GCTAAGTCTC TTCAGTCAGT CCCATCTTCC 
ATTTCAGACG TTAATCTTCA GAATCAGGAT GAAAATATGC CAAGTCAGTA CCTTGCACAG 
TTGGCGGCAA AGCCTTTCTT CTGTGAGCTT CAAGGATGCA AATATGAGTT TGTGACCAGA 
GAAGCTTTGT TAATGCATTA TCTTAAAAAG CATAACTATT CAAAAGAGAA GGTCCTTCAG 
TTAACCATGT TTCAGCACCG ATATTCCCCA TTCCGGTGTC ACATCTGCCA AAGGTCATTT 
ACAAGAAAAA CACATCTTAG GATTCATTAT AAAAACAAAC ATCAAATTGG CAGTGACAGG 
GCAACTCACA GACTGTTAGA CAGTGAAAAA TGTGATCATG AAGGGCCATG CTCAGTAGAC 
AGACTGAAAG GTGACTGCTC TACAGAACTT GGTCCCAACA GTAATTCAGA GACCACGCAA 
TGTCATTCTA AAAAAGATGA ATGCAGCTCA GAAACAGACT TGGAGTCCTC TTGTGAGGAA 
ACAGAAAGTA AGATCTCTGG TATCTCATCA CCAATAGGTA GCCATAGAGA AGAGGGAGAG 
GAGAAAGAGG GGAGAGGAAG TAGGCGGACT GTTGCTAAAG GAAATCTGTG CTATATTTTG 
AATAAGTACC ACAAACCATT CCATTGTATC CACAAAACTT GCAATTCTTC ATTTACCAAT 
CTAAAAGGCC TGATTCGCCA TTACAGAACT GTGCATCAGT ACAACAAAGA GCAGTTATGC 
TTAGAGAAGG ACAAAGCAAG AACCAAAAGG GAGCTTGTAA AATGTAAAAA GCTATTTGCT 
TGCAAATATA AAGACTGTAA CAAGCGCTTC CTGTGCTCCA AGGCCCTGGC TAAGCACTGT 
AGTGACTCTC ATAACCTAGA CCACATTGAA GAGTCTAAAG TGCTTTCTGA GACGGAGTCT 
GCAGCGAGGT TTTCTTGTAA CCAGCCTCAG TGCCCTGCTG TTTTTTATTC ATTCAGTAAG 
TTGAAACACC ACCTGTTGGA ACAGCATAAT ATTGAAGGAG AAATCCATTC GGATTATGAG 
ATCCATTGTA CTCTTAATGG CTGTGGCCAG ATTTTCAGCC ATCGAAGTAA TTACTTCCAG 
CATGTCTACT ACCGACATAA GGACTATTAT GACAACCTGT TCAGCAGCCA GAAAGTAGCA 
AATGAGCGGC TTCTAAGGAG TGAAAAGGTG TGTCAGACAA CTCAGGCACA AGGACTGACG 
CAGACGCAGG GGCAGACACA GGGACAGACG CAGGGACAGG AACAGCAGGC TGCCAAAAGG 
CCATTTAATA CTAAAGCTAA AAAATGTGGC TTACTCAAAG ATAAAAAAGC TCCAATTACC 
TTTAAAACAA GAGCAGAAGC GATCCATATG TGTGTTGAGC ATTCTGAGCA CACACAGTAC 
CCGTGCATGG TTCAGGGATG CTTGTCTGTG GTGAAGCTGG AAAGCAGCAT TGTGAGACAT 
TACAAACGTA CCCACCAGAT GAATAGTGCC TATTTAGAGC AGCAGTTGGA GAACCTTGTG 
GTTTGTGTTA AGTATGGTAC CAAAATTAAA GACGAGCCCC CTTCAGAAGT AGAGCCCTGT 
GTAAAGAAAG AGGAAAGTAC TAGCTGTGAA TCGGTGCACA CAGAGAATGG AGCCCCAGGA 
GACAGCAGTG TGCCCCTCCC AAACACTGAT TCTACTTGCC CAGCTGAACA AGATGTCGGT 
CAGAAAGGGT GTTCAGAAAG AAACCCAGTT TTTGACACAC ACAGTCTGCT CTACAGGGGA 
ACTTTGAAAT GCAACCATTC TTCAGAAACC ACTTCTTTGG AACAGTGTAA TATAGCTCAG 
TCTTCTCCCT GTAAAATAGA AAGCCCCATA CCTAATCCCA GTGGGACAGA AAGTGGTACT 
TATTTCACGG ACTTCCAGCT GCCATTACCA AGGATCAAAG AAGAACCTGG GCAGCATAGT 
TCAGGGCAAG AAAACACTGT AAAGAATGCA ACCCAGGTCC CAAAAGAGAA TATTAGAAAG 
CATTCTCAGC CCAGGTCATT TGATCTGAAG ACTTACAAAC CTATGGGATT TGAGTCTTCC 
TTTCTGAAAT TTATTCAGGA AAGTGAAGAG AAGGATGATG ATTTTGATGA CTGGGAGCCT 
TCAGAGCACT TAACATTGAA TAACTCCTCT CACCCCAGTA ATGACTTGAC AGGGAATGTT 
GTGGCAGATA CCATAGTGAA TGAGAGTGCC CCTCAAGTTG ACATACCTCA TTCTTCCAGT 
GACCCTCCAG TTTCAGAGAA CCTGACTGCA GTCCCACCAT TAGTAGTAGC TGAAGCAACA 
GCAGTTCCTT CCTTGGAAAA CCTGAGGGTT GTGCTGGACA AAGCATTAAC AGACTGTGGA 
GAACTTGCCT TAAAACAGCT TCATTACCTT CGGCCTGTGG TTGTCCTTGA AAGATCTAAG 
TTCTCTACAC CAATTTTAGA CTTGTTTCCA ACTAAGAAGA CAGATGAGCT TTGTGTAGGA 
AGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of Rearranged L-Myc Fusion (RLF) results in defects in heart development in the mouse.
Differentiation; research in biological diversity948-20(2017 Mar - Apr)
Bourke LM,Del Monte-Nieto G,Outhwaite JE,Bharti V,Pollock PM,Simmons DG,Adam A,Hur SS,Maghzal GJ,Whitelaw E,Stocker R,Suter CM,Harvey RP,Harten SK

Control of cardiac jelly dynamics by NOTCH1 and NRG1 defines the building plan for trabeculation.
Nature557(7705)439-445(2018 05)
Del Monte-Nieto G,Ramialison M,Adam AAS,Wu B,Aharonov A,D'Uva G,Bourke LM,Pitulescu ME,Chen H,de la Pompa JL,Shou W,Adams RH,Harten SK,Tzahor E,Zhou B,Harvey RP

The recently identified modifier of murine metastable epialleles, Rearranged L-Myc Fusion, is involved in maintaining epigenetic marks at CpG island shores and enhancers.
BMC biology1321(2015 Mar)
Harten SK,Oey H,Bourke LM,Bharti V,Isbel L,Daxinger L,Faou P,Robertson N,Matthews JM,Whitelaw E

An ENU mutagenesis screen identifies novel and known genes involved in epigenetic processes in the mouse.
Genome biology14(9)R96(2013)
Daxinger L,Harten SK,Oey H,Epp T,Isbel L,Huang E,Whitelaw N,Apedaile A,Sorolla A,Yong J,Bharti V,Sutton J,Ashe A,Pang Z,Wallace N,Gerhardt DJ,Blewitt ME,Jeddeloh JA,Whitelaw E

A genome-wide screen for modifiers of transgene variegation identifies genes with critical roles in development.
Genome biology9(12)R182(2008)
Ashe A,Morgan DK,Whitelaw NC,Bruxner TJ,Vickaryous NK,Cox LL,Butterfield NC,Wicking C,Blewitt ME,Wilkins SJ,Anderson GJ,Cox TC,Whitelaw E

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Rlf cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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