LINJ_35_1780 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_35_1780
Entrez Gene ID	5073085
Full Name	putative DNA polymerase epsilon subunit b
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001468961.1	XP_001468998.1	putative DNA polymerase epsilon subunit b

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_35_1780 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_35_1780 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc06311	XM_001468961.1 Latest version!	Leishmania infantum JPCM5 putative DNA polymerase epsilon subunit b partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_35_1780 ( XM_001468961.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001468998.1 Leishmania infantum JPCM5 putative DNA polymerase epsilon subunit b partial mRNA.

Price & Availability↑

CloneID	OLc06311
Clone ID Related Accession (Same CDS sequence)	XM_001468961.1
Accession Version	XM_001468961.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative DNA polymerase epsilon subunit b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009419). COMPLETENESS: incomplete on both ends.

ATGAGGGAGC CCAAGGATGA GATAAGGAAG TTTGCGCAGG CGTACAAGTA CAACTTGAAG 
CCGTTGGCGG TCAGCCGCTT TGCCAGCTTC CTGAAAAAGT ATGACACTGG TGAGGAGTAC 
CGTCGCGATC TTCTGCGCGA CTTGTTCAGC TTGATTGGGG AACAATGCTG TGACAATCGA 
CTCGTTGATG AATTGAAGGC AGAAGCGGTC ATCAGCTTGC AAGAAGCAAA GATAAAAGGA 
TGCCTAGGCA CCGACGGGCC ATCTTCCGTG CAAGTCGTCT CCCTGGGTGC TGTTCCCAAA 
GTCTTTGTCG ACGAGGTAAG TAACGAACTG AGGGTGAGTA GTTCCTCGGT TGCATTAAAC 
CGTCTTCAGT GCCTCAATCA ACGCTATGCA CTCGCTCGTC GGCGTTGCTT GCGATGTGGC 
CTCTACCACA AATCAGTGGA TCAGCATGCG CAACATGACG GCTCCCTGGC TCTTTTGCCC 
ATCTCCGCGC TTGAGGGGCT CGATCCAGGC CTCCAAGTCG CTGTGCTTGG GCTCTTGGTC 
GTGCAGGATG ACGAAGTATT CTTGGAGGAT TTAAGGGGAC AGATTAAACT GAAATTCAGC 
GAGGAGTCGC GGGTCACGCC GCACGGCTTT GTGGGAAATG GGTTCCTGAC TGTGGTGAGC 
GGATTCTGGG TGAACGGCAC TCTCCAGGTC ACTCGAGTGG ACCTCCCGCC GGCAGAGCGG 
CGGGAGGTGA CGTTGCGGGA TGCCGGCTCT AGCATGGACC TATTCGGGTT GGCCCCGCCC 
AACCCCATGG AGGCTTTGCG AGAGGAGAAG AAGCGGGTGC AGAGCGTGAT CATCGTCATG 
GCGCACGTGC ACCTCGACAA GGCGGGCACC ATGAGCAAGC TGGCTCGCTT TTTTGAGCGC 
ATGCAGGGCT TAAGTGAGTC AGAGCTGCGC GACGTCACTT TTGTCTTCAC CGGAGACTTT 
TCGTCCTTCC CGCTTCACTT TGGCGACGCG TCTCACTTGC CTGATATCTT CGACAACTCG 
GACCGCTTTC AGATCCTCTT AGACGGACTA GGTCGCTGCA TCGCCGCCAA TGCTCCCACT 
GCAGCACAGC ACAGTCATTT TGTGCTTATC CCGGGCCCCA GCGACATGAC TGCCTTGCAG 
GGCTTTCAGC CGCAAACGCC CATTGCCACA CAATTTGCCA AGGGTGTGGA GAGTCGAACA 
AAGAAGCTCA CTCTTGCTCC GAATCCGTGC CGGTTGCGCT TTCACACACA CGAGGCTATC 
ATCGCTCGCC GTGATTTCCT GCGCGATCTC CAGCGCGGCG AGTTGCTCTA TCCGTGGGAC 
CGCTATCGCA CCACGGAGCG TACCGACGCG GCACTGGATC AGGGCGCTTC TAGTGGGGCT 
GGGGCAGCCA CCACAACGTC GTTTGAGCGC GTTACCAAGA CACTGCTAGA CGAGGCACAC 
CTGTCACCCA GCATTGATGA GGCGGTTTTG TGGAGAGCAG ACGACGCCTT GCGCATCCCG 
GTGCTGCCGC ACACGATGCT GCTGTGCGAC TCAACAGAGC AATGGGAGTG CCATTACAAA 
GGCGTTCATG TCGTGAACCC CGGCTCCTTT GCCGTCAACG GCACATTCCT TTGGTACACC 
CCAGCAGACG GGCAGTGCAG CCTCAATAGA CTGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001468998.1
CDS	1..1659
Translation	MREPKDEIRKFAQAYKYNLKPLAVSRFASFLKKYDTGEEYRRDLLRDLFSLIGEQCCDNRLVDELKAEAVISLQEAKIKGCLGTDGPSSVQVVSLGAVPKVFVDEVSNELRVSSSSVALNRLQCLNQRYALARRRCLRCGLYHKSVDQHAQHDGSLALLPISALEGLDPGLQVAVLGLLVVQDDEVFLEDLRGQIKLKFSEESRVTPHGFVGNGFLTVVSGFWVNGTLQVTRVDLPPAERREVTLRDAGSSMDLFGLAPPNPMEALREEKKRVQSVIIVMAHVHLDKAGTMSKLARFFERMQGLSESELRDVTFVFTGDFSSFPLHFGDASHLPDIFDNSDRFQILLDGLGRCIAANAPTAAQHSHFVLIPGPSDMTALQGFQPQTPIATQFAKGVESRTKKLTLAPNPCRLRFHTHEAIIARRDFLRDLQRGELLYPWDRYRTTERTDAALDQGASSGAGAATTTSFERVTKTLLDEAHLSPSIDEAVLWRADDALRIPVLPHTMLLCDSTEQWECHYKGVHVVNPGSFAVNGTFLWYTPADGQCSLNRLE

Target ORF information:

RefSeq Version	XM_001468961.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative DNA polymerase epsilon subunit b partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001468961.1

ORF Insert Sequence:

ATGAGGGAGC CCAAGGATGA GATAAGGAAG TTTGCGCAGG CGTACAAGTA CAACTTGAAG 
CCGTTGGCGG TCAGCCGCTT TGCCAGCTTC CTGAAAAAGT ATGACACTGG TGAGGAGTAC 
CGTCGCGATC TTCTGCGCGA CTTGTTCAGC TTGATTGGGG AACAATGCTG TGACAATCGA 
CTCGTTGATG AATTGAAGGC AGAAGCGGTC ATCAGCTTGC AAGAAGCAAA GATAAAAGGA 
TGCCTAGGCA CCGACGGGCC ATCTTCCGTG CAAGTCGTCT CCCTGGGTGC TGTTCCCAAA 
GTCTTTGTCG ACGAGGTAAG TAACGAACTG AGGGTGAGTA GTTCCTCGGT TGCATTAAAC 
CGTCTTCAGT GCCTCAATCA ACGCTATGCA CTCGCTCGTC GGCGTTGCTT GCGATGTGGC 
CTCTACCACA AATCAGTGGA TCAGCATGCG CAACATGACG GCTCCCTGGC TCTTTTGCCC 
ATCTCCGCGC TTGAGGGGCT CGATCCAGGC CTCCAAGTCG CTGTGCTTGG GCTCTTGGTC 
GTGCAGGATG ACGAAGTATT CTTGGAGGAT TTAAGGGGAC AGATTAAACT GAAATTCAGC 
GAGGAGTCGC GGGTCACGCC GCACGGCTTT GTGGGAAATG GGTTCCTGAC TGTGGTGAGC 
GGATTCTGGG TGAACGGCAC TCTCCAGGTC ACTCGAGTGG ACCTCCCGCC GGCAGAGCGG 
CGGGAGGTGA CGTTGCGGGA TGCCGGCTCT AGCATGGACC TATTCGGGTT GGCCCCGCCC 
AACCCCATGG AGGCTTTGCG AGAGGAGAAG AAGCGGGTGC AGAGCGTGAT CATCGTCATG 
GCGCACGTGC ACCTCGACAA GGCGGGCACC ATGAGCAAGC TGGCTCGCTT TTTTGAGCGC 
ATGCAGGGCT TAAGTGAGTC AGAGCTGCGC GACGTCACTT TTGTCTTCAC CGGAGACTTT 
TCGTCCTTCC CGCTTCACTT TGGCGACGCG TCTCACTTGC CTGATATCTT CGACAACTCG 
GACCGCTTTC AGATCCTCTT AGACGGACTA GGTCGCTGCA TCGCCGCCAA TGCTCCCACT 
GCAGCACAGC ACAGTCATTT TGTGCTTATC CCGGGCCCCA GCGACATGAC TGCCTTGCAG 
GGCTTTCAGC CGCAAACGCC CATTGCCACA CAATTTGCCA AGGGTGTGGA GAGTCGAACA 
AAGAAGCTCA CTCTTGCTCC GAATCCGTGC CGGTTGCGCT TTCACACACA CGAGGCTATC 
ATCGCTCGCC GTGATTTCCT GCGCGATCTC CAGCGCGGCG AGTTGCTCTA TCCGTGGGAC 
CGCTATCGCA CCACGGAGCG TACCGACGCG GCACTGGATC AGGGCGCTTC TAGTGGGGCT 
GGGGCAGCCA CCACAACGTC GTTTGAGCGC GTTACCAAGA CACTGCTAGA CGAGGCACAC 
CTGTCACCCA GCATTGATGA GGCGGTTTTG TGGAGAGCAG ACGACGCCTT GCGCATCCCG 
GTGCTGCCGC ACACGATGCT GCTGTGCGAC TCAACAGAGC AATGGGAGTG CCATTACAAA 
GGCGTTCATG TCGTGAACCC CGGCTCCTTT GCCGTCAACG GCACATTCCT TTGGTACACC 
CCAGCAGACG GGCAGTGCAG CCTCAATAGA CTGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_35_1780 cDNA ORF clone, Leishmania infantum JPCM5

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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