LINJ_27_0740 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_27_0740
Entrez Gene ID	5070037
Full Name	putative 2-oxoglutarate dehydrogenase subunit
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001466292.2	XP_001466329.2	putative 2-oxoglutarate dehydrogenase subunit

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_27_0740 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_27_0740 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc03638	XM_001466292.2 Latest version!	Leishmania infantum JPCM5 putative 2-oxoglutarate dehydrogenase subunit partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_27_0740 ( XM_001466292.2 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001466329.2 Leishmania infantum JPCM5 putative 2-oxoglutarate dehydrogenase subunit partial mRNA.

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CloneID	OLc03638
Clone ID Related Accession (Same CDS sequence)	XM_001466292.2
Accession Version	XM_001466292.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative 2-oxoglutarate dehydrogenase subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009411). On Jul 14, 2011 this sequence version replaced XM_001466292.1. COMPLETENESS: incomplete on both ends.

ATGATGCGCC GCGCATTGAG TGGTGCCGTC GCCGTCCGCG CGTCGGCGAT GCGCAGTTAT 
ACGGACGCCC GCACGATCCG CAAGCCGAAC CCATATGACC AGCTCGTCAA CGCCGAGAAC 
CAGCACTACG TGGAGGATCT CATGCGACAG TACGAGGCTG ACAGCGCTCT TGTCGATCCC 
AGCTGGGTGC CCGTGCTGGA GGCAATTCGG TCCGGAAGTG ACGATTCTCC GGTTGTGGCG 
ACGTTCAGCC GCCCGACAGA CGCCAAGTCA TTGTCGGAAA AGCAGCGCCA CGACAATATG 
CGCCTTTCGT GGATGATCCG CGAATACGAG CGGTTTGGCC ACCATATGGC GAACGTGGAC 
CCGCTCAGCG GCTACCACGC CGATAATCGT ATCCTGGGAT CTCGCACACT GGCGCCGGAG 
GAGTTCGGCT TCACCAAGGA TGATCTGACG CATGTCTTCA ACGTCACTTT TGGCGCCAGC 
CACGAGGCGA CCTTTGTCAG CGGTGGCACT GCCATGACGC TGCAGCAGAT TATCGATCAA 
CTCCGCCGGC TCTACTGTGG ACCGATCGGG TTCGAGTTCA TGTCGTCGGG CTTCTTCGAG 
CTGCGCAACT GGTTCCGGCA GGAGGTCATG GACTCCCTGC AGCCCTTGCC AGCCGAGGAG 
CGTAGGCTTT ACTACAACGA CGTCGTGAAG GCCTGTGGCT TCGAGAAGTT TCTGCAGTTG 
AAGTACGCCA CAAAGCATCG CTTCGGCCTC GACGGCGGCG AGGCGCTCAT TCCGGCCTTG 
AAGGCCGCCA TCCTCACGTC GAGCGACCTC GGCGTGCAGA GCGCGATCAT CGGCATGGCA 
CACCGCGGCC GCCTGAATGT CCTGGCGAAC GTGCTGCGCA AGTCCCTGCG CGCCATCCTG 
AACGAGTTCG AAGGCCGCGT GGCGATCGAG GATGCGCACC TCACCGGCGA CGTCGAGTAC 
CATCTCGGCA AGCGCAAGCA CGTGAAACTG CCAAACAACA AGTCCATCGA GCTGGACCTG 
CTGCCGAACC CCTCCCATCT CGAAGCCGTA AACCCGCTGG TGCTGGGTAA GGCGCGTGCG 
CGGCAAATCT ACACGAACGA CGTGGAGTGC ACGGCGGTGC TGCCGATCCT CATCCACGGC 
GACGCCGCAT TCGCCGGTCA AGGGTCGTGC TACGAGACGA TGGGCTTCTG CGAGCTAGAA 
AACTTCCACG TCGGTGGCAC GCTGCATTTG GTCATCAACA ACCAGATTGG ATTCACCACC 
AACCCGAAGG ACTCGCGCGC CAGCGCCTAC TGCACCGACC TGTCAAAGGT GAACAACGCC 
CCCGTGATGC ACGTCAACGG TGATGACGTG GACGCGTGCG TGAAGGCGGC CAAGATTGCC 
GCACGCTTCC GCCAGCAGTT CCACCACGAC ATCATCATCG ACCTCGTCTG CTACCGCCGC 
TACGGACACA ACGAAACCGA CCTGCCCGAC TTTACCCAGC CGCAGCTGTA CAACCAGATT 
CGCCAGCACC CCAGCGTCGT AGACATTTAC ACCAAGACCC TCATCAGGGA CGGCGTGCTG 
ACGGCAGAGG AGGCCAAAGC GAAGGACAAG GAATGGGAGG GCGTCCTGCG CCAGGCGTAC 
GACCGCATGA ACAGCGCACA GAACTTCGTG AAAGTCATGC CCGTGTTTGA CCCCGAGAGC 
GAGAACACCT CGGCCGATTT GTCGTACGCG AAGATCGCTG CCACACGTGT GCCGCCGCCG 
GTTTCGGCGG TGGACACCGG CGTCGAGACT CAGACGCTAC GCATGGCCGG CTTGCGCCTG 
GCGTCGATCC CGAAGGAGAT GCAGAAGCCG CACCCGGTGG TGGAACGGAC GTACGCGGCG 
CGCAAGAAGG GCACGGAGCA GGGGGACGCA ATCGAGTGGT GTCAGGCAGA GCTCATGGCG 
TTGGCGACGC TCTCAATGCA AGGGGTGCCG ATCCGGCTGA CTGGCGAGGA TGTCGAGCGC 
GGCACCTTCA CCCAGCGCCA CGCCGGCATT ACGGACATGA AGACGAACCT CAAGTACTTC 
CCGGTGAAGA CGCTGTCGCC TTCGCAGGCC CTCATCACAA TCTCCAACAG CTCCCTCTCT 
GAGCTCGGCG TCTGCGGCTT TGAGATGGGG TACAACATGG AGAACACCCG CTCCATCACC 
ATTTGGGAGG CCCAGTTCGG CGACTTCGCT AACGGTGCAC AGGTGATCTT CGACCAGTTC 
CTCAGCTGCT GCGAGGAGAA GTGGAATGAG CACAGTAGCC TCGTCCTGTC GCTGCCGCAC 
GGCTACTCGG GCGCCGGCCC CGAGCACAGC TCTGCTCGCG TGGAGCGCTT CCTGCAGCTG 
AGCGACGACG GCGACCGAGT GCCATCGGAC TTTCGCCACT TCCCCAACGA CCAGGCGCTC 
GAAATCCGCA TCCGTCGCCA CAACTGGCAG GTGACCTACC CCAGCACTCC GGCGAACTAC 
TTTCATCTTC TGCGCCGCCA AGGACTCCGC GAATTTGCGA AGCCGCTCAT CAATTTCTTC 
TCGAAGGCGC GCCTGCGTGC GCCGAACCTA TCAAAGCTGT CCGACATGAC TCAGGGGACC 
AGTTTCAAGG CGGTCATTGA CACCGCACGC GCCAAGGACA CGGTGGCCCG CAAGGTCGTG 
TTCTGCTCTG GCCAAATCGA GAGCATTGTG AATGATGCAA AGGCCGCCAG GCAGAAGGAG 
ACGCCAGGCG TGCACGACGA CGTCGTCCTC GTCACAGTCG AGCAGCTGGC TCCGTTCCCG 
TGGGAACAGG TGGCGGATGT GATGGAGAAG TACGCGCAGC GTAACCTCAA CACGGAGTTT 
GTGTGGCTGC AGGAGGAGCC ACGCAACATG GGCATGTGGA CGCACATGCG GCCCCGCATG 
AACAGCCTGA TGCACCATCT CGGCCTCAAG CAGAAGCGCA TTAACGTCGT GAGCCGCCCG 
TCTGCAGCTT CGCCGTCCAC GGGCTACGGC TCCGTGCACG TGGCGGAGGA GAAGAAGCTT 
ATCGCGGAAA CCCTCGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001466329.2
CDS	1..3021
Translation	MMRRALSGAVAVRASAMRSYTDARTIRKPNPYDQLVNAENQHYVEDLMRQYEADSALVDPSWVPVLEAIRSGSDDSPVVATFSRPTDAKSLSEKQRHDNMRLSWMIREYERFGHHMANVDPLSGYHADNRILGSRTLAPEEFGFTKDDLTHVFNVTFGASHEATFVSGGTAMTLQQIIDQLRRLYCGPIGFEFMSSGFFELRNWFRQEVMDSLQPLPAEERRLYYNDVVKACGFEKFLQLKYATKHRFGLDGGEALIPALKAAILTSSDLGVQSAIIGMAHRGRLNVLANVLRKSLRAILNEFEGRVAIEDAHLTGDVEYHLGKRKHVKLPNNKSIELDLLPNPSHLEAVNPLVLGKARARQIYTNDVECTAVLPILIHGDAAFAGQGSCYETMGFCELENFHVGGTLHLVINNQIGFTTNPKDSRASAYCTDLSKVNNAPVMHVNGDDVDACVKAAKIAARFRQQFHHDIIIDLVCYRRYGHNETDLPDFTQPQLYNQIRQHPSVVDIYTKTLIRDGVLTAEEAKAKDKEWEGVLRQAYDRMNSAQNFVKVMPVFDPESENTSADLSYAKIAATRVPPPVSAVDTGVETQTLRMAGLRLASIPKEMQKPHPVVERTYAARKKGTEQGDAIEWCQAELMALATLSMQGVPIRLTGEDVERGTFTQRHAGITDMKTNLKYFPVKTLSPSQALITISNSSLSELGVCGFEMGYNMENTRSITIWEAQFGDFANGAQVIFDQFLSCCEEKWNEHSSLVLSLPHGYSGAGPEHSSARVERFLQLSDDGDRVPSDFRHFPNDQALEIRIRRHNWQVTYPSTPANYFHLLRRQGLREFAKPLINFFSKARLRAPNLSKLSDMTQGTSFKAVIDTARAKDTVARKVVFCSGQIESIVNDAKAARQKETPGVHDDVVLVTVEQLAPFPWEQVADVMEKYAQRNLNTEFVWLQEEPRNMGMWTHMRPRMNSLMHHLGLKQKRINVVSRPSAASPSTGYGSVHVAEEKKLIAETLA

Target ORF information:

RefSeq Version	XM_001466292.2
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative 2-oxoglutarate dehydrogenase subunit partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001466292.2

ORF Insert Sequence:

ATGATGCGCC GCGCATTGAG TGGTGCCGTC GCCGTCCGCG CGTCGGCGAT GCGCAGTTAT 
ACGGACGCCC GCACGATCCG CAAGCCGAAC CCATATGACC AGCTCGTCAA CGCCGAGAAC 
CAGCACTACG TGGAGGATCT CATGCGACAG TACGAGGCTG ACAGCGCTCT TGTCGATCCC 
AGCTGGGTGC CCGTGCTGGA GGCAATTCGG TCCGGAAGTG ACGATTCTCC GGTTGTGGCG 
ACGTTCAGCC GCCCGACAGA CGCCAAGTCA TTGTCGGAAA AGCAGCGCCA CGACAATATG 
CGCCTTTCGT GGATGATCCG CGAATACGAG CGGTTTGGCC ACCATATGGC GAACGTGGAC 
CCGCTCAGCG GCTACCACGC CGATAATCGT ATCCTGGGAT CTCGCACACT GGCGCCGGAG 
GAGTTCGGCT TCACCAAGGA TGATCTGACG CATGTCTTCA ACGTCACTTT TGGCGCCAGC 
CACGAGGCGA CCTTTGTCAG CGGTGGCACT GCCATGACGC TGCAGCAGAT TATCGATCAA 
CTCCGCCGGC TCTACTGTGG ACCGATCGGG TTCGAGTTCA TGTCGTCGGG CTTCTTCGAG 
CTGCGCAACT GGTTCCGGCA GGAGGTCATG GACTCCCTGC AGCCCTTGCC AGCCGAGGAG 
CGTAGGCTTT ACTACAACGA CGTCGTGAAG GCCTGTGGCT TCGAGAAGTT TCTGCAGTTG 
AAGTACGCCA CAAAGCATCG CTTCGGCCTC GACGGCGGCG AGGCGCTCAT TCCGGCCTTG 
AAGGCCGCCA TCCTCACGTC GAGCGACCTC GGCGTGCAGA GCGCGATCAT CGGCATGGCA 
CACCGCGGCC GCCTGAATGT CCTGGCGAAC GTGCTGCGCA AGTCCCTGCG CGCCATCCTG 
AACGAGTTCG AAGGCCGCGT GGCGATCGAG GATGCGCACC TCACCGGCGA CGTCGAGTAC 
CATCTCGGCA AGCGCAAGCA CGTGAAACTG CCAAACAACA AGTCCATCGA GCTGGACCTG 
CTGCCGAACC CCTCCCATCT CGAAGCCGTA AACCCGCTGG TGCTGGGTAA GGCGCGTGCG 
CGGCAAATCT ACACGAACGA CGTGGAGTGC ACGGCGGTGC TGCCGATCCT CATCCACGGC 
GACGCCGCAT TCGCCGGTCA AGGGTCGTGC TACGAGACGA TGGGCTTCTG CGAGCTAGAA 
AACTTCCACG TCGGTGGCAC GCTGCATTTG GTCATCAACA ACCAGATTGG ATTCACCACC 
AACCCGAAGG ACTCGCGCGC CAGCGCCTAC TGCACCGACC TGTCAAAGGT GAACAACGCC 
CCCGTGATGC ACGTCAACGG TGATGACGTG GACGCGTGCG TGAAGGCGGC CAAGATTGCC 
GCACGCTTCC GCCAGCAGTT CCACCACGAC ATCATCATCG ACCTCGTCTG CTACCGCCGC 
TACGGACACA ACGAAACCGA CCTGCCCGAC TTTACCCAGC CGCAGCTGTA CAACCAGATT 
CGCCAGCACC CCAGCGTCGT AGACATTTAC ACCAAGACCC TCATCAGGGA CGGCGTGCTG 
ACGGCAGAGG AGGCCAAAGC GAAGGACAAG GAATGGGAGG GCGTCCTGCG CCAGGCGTAC 
GACCGCATGA ACAGCGCACA GAACTTCGTG AAAGTCATGC CCGTGTTTGA CCCCGAGAGC 
GAGAACACCT CGGCCGATTT GTCGTACGCG AAGATCGCTG CCACACGTGT GCCGCCGCCG 
GTTTCGGCGG TGGACACCGG CGTCGAGACT CAGACGCTAC GCATGGCCGG CTTGCGCCTG 
GCGTCGATCC CGAAGGAGAT GCAGAAGCCG CACCCGGTGG TGGAACGGAC GTACGCGGCG 
CGCAAGAAGG GCACGGAGCA GGGGGACGCA ATCGAGTGGT GTCAGGCAGA GCTCATGGCG 
TTGGCGACGC TCTCAATGCA AGGGGTGCCG ATCCGGCTGA CTGGCGAGGA TGTCGAGCGC 
GGCACCTTCA CCCAGCGCCA CGCCGGCATT ACGGACATGA AGACGAACCT CAAGTACTTC 
CCGGTGAAGA CGCTGTCGCC TTCGCAGGCC CTCATCACAA TCTCCAACAG CTCCCTCTCT 
GAGCTCGGCG TCTGCGGCTT TGAGATGGGG TACAACATGG AGAACACCCG CTCCATCACC 
ATTTGGGAGG CCCAGTTCGG CGACTTCGCT AACGGTGCAC AGGTGATCTT CGACCAGTTC 
CTCAGCTGCT GCGAGGAGAA GTGGAATGAG CACAGTAGCC TCGTCCTGTC GCTGCCGCAC 
GGCTACTCGG GCGCCGGCCC CGAGCACAGC TCTGCTCGCG TGGAGCGCTT CCTGCAGCTG 
AGCGACGACG GCGACCGAGT GCCATCGGAC TTTCGCCACT TCCCCAACGA CCAGGCGCTC 
GAAATCCGCA TCCGTCGCCA CAACTGGCAG GTGACCTACC CCAGCACTCC GGCGAACTAC 
TTTCATCTTC TGCGCCGCCA AGGACTCCGC GAATTTGCGA AGCCGCTCAT CAATTTCTTC 
TCGAAGGCGC GCCTGCGTGC GCCGAACCTA TCAAAGCTGT CCGACATGAC TCAGGGGACC 
AGTTTCAAGG CGGTCATTGA CACCGCACGC GCCAAGGACA CGGTGGCCCG CAAGGTCGTG 
TTCTGCTCTG GCCAAATCGA GAGCATTGTG AATGATGCAA AGGCCGCCAG GCAGAAGGAG 
ACGCCAGGCG TGCACGACGA CGTCGTCCTC GTCACAGTCG AGCAGCTGGC TCCGTTCCCG 
TGGGAACAGG TGGCGGATGT GATGGAGAAG TACGCGCAGC GTAACCTCAA CACGGAGTTT 
GTGTGGCTGC AGGAGGAGCC ACGCAACATG GGCATGTGGA CGCACATGCG GCCCCGCATG 
AACAGCCTGA TGCACCATCT CGGCCTCAAG CAGAAGCGCA TTAACGTCGT GAGCCGCCCG 
TCTGCAGCTT CGCCGTCCAC GGGCTACGGC TCCGTGCACG TGGCGGAGGA GAAGAAGCTT 
ATCGCGGAAA CCCTCGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_27_0740 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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