LINJ_06_0260 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_06_0260
Entrez Gene ID	4961290
Full Name	putative 5'-3' exonuclease
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001463071.2	XP_001463108.2	putative 5'-3' exonuclease

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_06_0260 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_06_0260 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc00017	XM_001463071.2 Latest version!	Leishmania infantum JPCM5 putative 5'-3' exonuclease partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_06_0260 ( XM_001463071.2 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001463108.2 Leishmania infantum JPCM5 putative 5'-3' exonuclease partial mRNA.

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CloneID	OLc00017
Clone ID Related Accession (Same CDS sequence)	XM_001463071.2
Accession Version	XM_001463071.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative 5'-3' exonuclease
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009277). On Jul 14, 2011 this sequence version replaced XM_001463071.1. COMPLETENESS: incomplete on both ends.

ATGGGTGTTC CCAAGTTCTT CCGCTGGGCG GCGGAGCGCT TCCCCTCCAT CATCACCCCC 
TTCAAGGACT CGCCACCGCC GGTGGACAAC CTCTACCTCG ATATCAATGG TATCATCCAC 
AACTGCACGC ACCCGAACGA TGTCGACGCG ACGCGGCGCA GTCCGACAGA GAAGGAGATG 
ATTCAGGCGA TGTTCGTCTA TCTGGAGAAA CTCTTCAACG CTATCCAGCC GCGCAAGTAC 
TTCCTGCTTG CCGTCGATGG TGTCGCGCCG CGCGCGAAAA TGAATCAGCA GCGCCAGCGC 
CGCTACCGCG CCGGTTACGA GATGATGATC GCGCGTGAGG AGGCGCTCGC GATGGGCGAG 
GAGGTGCCGG AGGAAAAGGA CGTGTTCGAC AGCAACTGCA TCACCCCCGG CACCCCGTTC 
ATGGTGCGCG TGAGCAAGGA GTTTCAGTAC TTTATCACCA TGAAGCTGTC CACCGATCCG 
GCGTGGCAGG GGTGCCAGAT CATCTTCTCC GGCCACGATT GCCCCGGCGA GGGTGAGCAC 
AAGATCGTCG ATTTTATTCG CCGGCGCAAG ATGCAGCCGA ACTACGACCC CAACGAGACC 
CACTGCATGT ACGGCCTCGA CGCCGATCTC GTCATGCTGG CGCTCGCCAC GCACGAGCCG 
CACTTTGTGC TGCTGCGCGA GGTGGTCACG TTCGGCCCCG GCTCTCGCAA GGAGCGGGAG 
CGGCAGGAGG AGGATGAGGC GAAGGGCCTC GTGAAGGACA AGGCGTACCA CAAGGCGGAC 
GAGTTCGTGC TGCTGCACAT CAACGTCTTC CGCGACTACC TGCAGCTGGA GGTGAAGGGC 
TACCTGGCGA AGGCGAAGAG CAGCGTGAGT ATCGACTTTG AGCGAGTGAT CGACGACTTC 
GTCTTCATGT GCTTCTTTAT CGGCAACGAC TTTCTGCCGA CGCTGCCGAC CATCGGCATT 
AACGATGGCA GCATACCGGC GCTCCTGCGC ATCTACGTGG ACAACATGCT CAGCAAGAGC 
AAGTACCTGA CGCACAAAGG CGAGATCGAC TGGCGCAACG CCGAGGTGTG GTTGAACGAG 
GTCGGCAAGC TCGAGTTCGA GACGCTGCGG CGACGTCAGC AGGAGGAGGC AGAGTTCCAG 
AAGAACCGCG CTCGCCGCGA CCCTACCCAC GAGGTGGACA CCGCCTCCGT AATACCTATC 
ACGTCGATCC TGGAGTTCAA ACAGCGCTAC TACGGCGAGA AGCACGGCTT TGCTGGCGGC 
TGGGACGCGA ACAGCGAAGA CATGCAACGA CTGCGGGAGC ACTACGTGGA GGGGCTCATG 
TGGGTCATGC ATTACTACTA TCAAGGCGTG CCTTCATGGA AGTGGTTCTT TCCGCACTAC 
TACGCACCCA TGGCGAGCGA CATGGTGAAC CTCCCCGCTA TCGCTGCCCG CGTCTCGTTC 
GAACTCGGCA AGCCCTTCCA GCCGCACCAG CAGCTGCTGG CCGTGCTGCC GCCCATGTCA 
TACCTGTCCA TGCCGAAGGC CTACTGGCCC CTGCTGCGCA GCCCAAGTAG CCCGCTCGCC 
AAGTACTTGC CCGAGCACAT CGTTATTGAC CGCGAGGGCG CGCGCGCGCC GTGGGAGGGC 
ATTGTGCTGA TCCCCTTCAT CGACGAGCGC ACGCTGCTCG CCGCCTACGA GTCGGTGCAG 
AAGAACGTCA GCCCCGAGGA CGCCGCGAAC AACGTGAATG GCCCCCCGCT GCGGTTCTGC 
TACGACGGCA AGATGCCGCC GTACGACCTG GAGGATGAGA TGTTCGGCTC TATCCGGAAT 
GTCCTCGTGA GGCGCGAGAC ATACGCGTTC CCTCACATCG ACAAGTTCAT TCCACAGCTG 
TGCCCTGGCG TGCACGTTGG CACGCGGCAG CTGGAGGGCT TCAGCACCCT CCAGTCGAAG 
CGCGACCTAA TCCAGCCGGT CTTCGAGGCT GGGGTCGTGT CGATCTTTGG CCGTCCCACC 
AAGAACGACA GTCTCATCCT GCAGATGCGC GACTTCTTCA AGGCCAAGTC AGTCGACCAG 
GTGGCGACTC TCGTCGGCAA GGAGGTGCTC GTCGGCTTCC CGCACTACAA GCGTGCCCGC 
ATCGCCTCCC TCAAGGATGT GCACAAGTCC ATCACTGCTT CGTACAGCAA GGAGGGCACC 
TTCAGCGGCA CCGAGGAGCA CAAGAACAAC CGCGAAGAGT CGACTGCCTT CGTGAAGGAG 
GCGGACACGC ACGCGCGCTT CATGAAGTCG CAGCTGGGCC TCGCCGTGGA GCAGGTCGAA 
GTTCTCGTGT ACGTGCACCG CTTCAACGGA ATGCAGGTGA CGCGCAAGGG GCACATTGAG 
CGACACTTCG CCACCACCTG GACGTGCTAC CCAATCCAGC TGATCTGCAC CTTGGATAAC 
ATAGACATGC AGGAGGACAG CCGTTACGTC GAGCGCGACC GCAGCGAAGG TGACAGCGCC 
TTTGGTGCCC GCTGCGTTTA CATCGGTCCT CAGCCCAAGT CGAAGAAGTC GCAGATGGAC 
GTGTACGGCA GCTGTGGCAC GGTTGTGACG GCTGGCCAGC ACGACACGAC GACGCAGCAG 
GGCGATCAGA TGACGGTTCG CCTCAAGGTG TTTCAGGAGC CGCTGCGGCT GCCGGCGGAG 
CTGCTGCAGT TTGCCGCACA GCGCAACTGG ACGGCGCTGC CGCAGGTGGC GATCAGTCTC 
GACATTTTAC CGCACACCTT GACTATCATC TGTAGCTCCA TCGTCACGTC GCCGCAGTTT 
GGCTCTCGCG AGCTGGGGCT GTGTCTAAAG TTCACTGGGC GGTGCATCGC CAAGGTCGGG 
TACGCGAAGC TGGTCCAGCA CAGTCTGAAC CCGTGGTACG TTGGGAACAA GGACATCTTT 
GCCCGCATGG AAAAGGATAC GGAGGACATT GGCTACCACT TGGAGATGGG CGAGAAGGGC 
GACTTCACGG GCAATTCGAG CAGCAACCGT GGTGGCAGCG GCACGTGGTA CTTCTCCCGC 
GACGCGCAGG AGCTGATCCG TGACTACGTG AAGCGGTTCC GCCCACTCAT CCAGTACATC 
GAGGCGGGCG GCGTAAACTC GAACAGCGTT GAGCCGCAGC AGTTCCTGAC GGGCAAGTGG 
CGCGACAGGG ATGTCGACGA CGTGCTAACG GAGATCGAGA CATTCTTAAT CGAGTCGGGG 
CTGCGCGAAA CGCCGATGAT CACGGCCACG GAGGAGGCGT TTCCGCAGCA GCTGCTCGAG 
CAGCTCGAGG CGTCGCTGCA GGAGCAGCAG AACCGCAGCT TCAAGGAGCT CTCGTTGCGG 
CAGGTAAGCC GCAATCAGCT CTACTTTCCT GTCACGCGCA GCAGCTGCGG CCATCTCGTG 
CCGCTACCAC TGCCGCAGGA GCAGACTTTC TACCTGGGCT CGCGGGTCGC GAACTGCCGC 
GCTACGGGCA TTGTTCCCTT CGGCACGCAA GGCACGATCG TGCGCTTGCT GGCCAGCGGC 
AGGGAAGCAG AGGTCGTCTA CGATACCCCC TTCACGGGAG GCACGCGACT CGATGGCCGC 
CTCAAGGACA CTCGCGCCGC CATCACGAAG CTGTCGGCGC TGCTTGTACT CAAGTCGACG 
GATGCGCCGC AGCCACAGCT GCAACCTCAG ACGCAGCCGG ACATGAGGGC CGCTGCAGCC 
GCCACCACGA ACGGGTCGGC CAAGCTGCGG GAGCTGGAAT TCCTCCTGCA GGCGAACGGC 
ATTCACGTGG ACCCCTCGGG CAAGTCCACA GTGTCGCCGC CATCGCTGCC TGCAGCGTTT 
CAGAGCCCCA CAAGTGCCGC GGCTCGCGCT GCGCCGGCCG ACTCTGGTGC GAGCCATAGC 
AGCACCGCAG GCGCCGGTGC AAGCGTTGTG CCGCGGCCGC GCAGTGCCGC TGCGACGGAG 
CAGACGCCGG CACCAGCGAC ACTAGGCAAG CGCGCCACCC CTGCCTCCGC CACCGCCCAC 
TTGTCCTCAA CGTCTTCGGT GACGCCAATC ACCAGAGAGA CCCACAGCAG TCCCGCGAGC 
CTCAAAATCA CGGCTGCCCC AGGGAGCAGC GGGATCAGCC TTCAAGACTT GGCCAAACAC 
TTGCCGGCCT CCACATCCCA GCGCTCGCCC GCCACTGTCG CCTCGCCGGC TGGCCACGCT 
CCAGCGTCGG GTGCAGTTGC GGCAACAACG GCGGCGGCGC TCAGCGGCAT CACCATCCCG 
GCCCCCAAGT CTGCGCAGCA GCACCAACAC CCGATGCAGC CCGCTTCGCC GCCGGCGACG 
ACAGCTGTGG CCTCGCCAAG CCAAAGCAAT GAGTCGAACG AGACTTCCCC CGGCTCAAAC 
ACGCGCCGCA AGTCGCGCGA CCTGCACGTC ATCCCAGACG ACTTCGTCAG TGGACGGTTC 
AAGATCAAGT ACACGAACAC GGGCTCGCTG TTCCGCAGCT GGCTGGACAC CTTCACGGCG 
GAGGCGGTGC GCAAGACCCT CGCCGAGCTG GAGGCGGAGG GGAATGCGAA TCCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001463108.2
CDS	1..4500
Translation	MGVPKFFRWAAERFPSIITPFKDSPPPVDNLYLDINGIIHNCTHPNDVDATRRSPTEKEMIQAMFVYLEKLFNAIQPRKYFLLAVDGVAPRAKMNQQRQRRYRAGYEMMIAREEALAMGEEVPEEKDVFDSNCITPGTPFMVRVSKEFQYFITMKLSTDPAWQGCQIIFSGHDCPGEGEHKIVDFIRRRKMQPNYDPNETHCMYGLDADLVMLALATHEPHFVLLREVVTFGPGSRKERERQEEDEAKGLVKDKAYHKADEFVLLHINVFRDYLQLEVKGYLAKAKSSVSIDFERVIDDFVFMCFFIGNDFLPTLPTIGINDGSIPALLRIYVDNMLSKSKYLTHKGEIDWRNAEVWLNEVGKLEFETLRRRQQEEAEFQKNRARRDPTHEVDTASVIPITSILEFKQRYYGEKHGFAGGWDANSEDMQRLREHYVEGLMWVMHYYYQGVPSWKWFFPHYYAPMASDMVNLPAIAARVSFELGKPFQPHQQLLAVLPPMSYLSMPKAYWPLLRSPSSPLAKYLPEHIVIDREGARAPWEGIVLIPFIDERTLLAAYESVQKNVSPEDAANNVNGPPLRFCYDGKMPPYDLEDEMFGSIRNVLVRRETYAFPHIDKFIPQLCPGVHVGTRQLEGFSTLQSKRDLIQPVFEAGVVSIFGRPTKNDSLILQMRDFFKAKSVDQVATLVGKEVLVGFPHYKRARIASLKDVHKSITASYSKEGTFSGTEEHKNNREESTAFVKEADTHARFMKSQLGLAVEQVEVLVYVHRFNGMQVTRKGHIERHFATTWTCYPIQLICTLDNIDMQEDSRYVERDRSEGDSAFGARCVYIGPQPKSKKSQMDVYGSCGTVVTAGQHDTTTQQGDQMTVRLKVFQEPLRLPAELLQFAAQRNWTALPQVAISLDILPHTLTIICSSIVTSPQFGSRELGLCLKFTGRCIAKVGYAKLVQHSLNPWYVGNKDIFARMEKDTEDIGYHLEMGEKGDFTGNSSSNRGGSGTWYFSRDAQELIRDYVKRFRPLIQYIEAGGVNSNSVEPQQFLTGKWRDRDVDDVLTEIETFLIESGLRETPMITATEEAFPQQLLEQLEASLQEQQNRSFKELSLRQVSRNQLYFPVTRSSCGHLVPLPLPQEQTFYLGSRVANCRATGIVPFGTQGTIVRLLASGREAEVVYDTPFTGGTRLDGRLKDTRAAITKLSALLVLKSTDAPQPQLQPQTQPDMRAAAAATTNGSAKLRELEFLLQANGIHVDPSGKSTVSPPSLPAAFQSPTSAAARAAPADSGASHSSTAGAGASVVPRPRSAAATEQTPAPATLGKRATPASATAHLSSTSSVTPITRETHSSPASLKITAAPGSSGISLQDLAKHLPASTSQRSPATVASPAGHAPASGAVAATTAAALSGITIPAPKSAQQHQHPMQPASPPATTAVASPSQSNESNETSPGSNTRRKSRDLHVIPDDFVSGRFKIKYTNTGSLFRSWLDTFTAEAVRKTLAELEAEGNANPL

Target ORF information:

RefSeq Version	XM_001463071.2
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative 5'-3' exonuclease partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001463071.2

ORF Insert Sequence:

ATGGGTGTTC CCAAGTTCTT CCGCTGGGCG GCGGAGCGCT TCCCCTCCAT CATCACCCCC 
TTCAAGGACT CGCCACCGCC GGTGGACAAC CTCTACCTCG ATATCAATGG TATCATCCAC 
AACTGCACGC ACCCGAACGA TGTCGACGCG ACGCGGCGCA GTCCGACAGA GAAGGAGATG 
ATTCAGGCGA TGTTCGTCTA TCTGGAGAAA CTCTTCAACG CTATCCAGCC GCGCAAGTAC 
TTCCTGCTTG CCGTCGATGG TGTCGCGCCG CGCGCGAAAA TGAATCAGCA GCGCCAGCGC 
CGCTACCGCG CCGGTTACGA GATGATGATC GCGCGTGAGG AGGCGCTCGC GATGGGCGAG 
GAGGTGCCGG AGGAAAAGGA CGTGTTCGAC AGCAACTGCA TCACCCCCGG CACCCCGTTC 
ATGGTGCGCG TGAGCAAGGA GTTTCAGTAC TTTATCACCA TGAAGCTGTC CACCGATCCG 
GCGTGGCAGG GGTGCCAGAT CATCTTCTCC GGCCACGATT GCCCCGGCGA GGGTGAGCAC 
AAGATCGTCG ATTTTATTCG CCGGCGCAAG ATGCAGCCGA ACTACGACCC CAACGAGACC 
CACTGCATGT ACGGCCTCGA CGCCGATCTC GTCATGCTGG CGCTCGCCAC GCACGAGCCG 
CACTTTGTGC TGCTGCGCGA GGTGGTCACG TTCGGCCCCG GCTCTCGCAA GGAGCGGGAG 
CGGCAGGAGG AGGATGAGGC GAAGGGCCTC GTGAAGGACA AGGCGTACCA CAAGGCGGAC 
GAGTTCGTGC TGCTGCACAT CAACGTCTTC CGCGACTACC TGCAGCTGGA GGTGAAGGGC 
TACCTGGCGA AGGCGAAGAG CAGCGTGAGT ATCGACTTTG AGCGAGTGAT CGACGACTTC 
GTCTTCATGT GCTTCTTTAT CGGCAACGAC TTTCTGCCGA CGCTGCCGAC CATCGGCATT 
AACGATGGCA GCATACCGGC GCTCCTGCGC ATCTACGTGG ACAACATGCT CAGCAAGAGC 
AAGTACCTGA CGCACAAAGG CGAGATCGAC TGGCGCAACG CCGAGGTGTG GTTGAACGAG 
GTCGGCAAGC TCGAGTTCGA GACGCTGCGG CGACGTCAGC AGGAGGAGGC AGAGTTCCAG 
AAGAACCGCG CTCGCCGCGA CCCTACCCAC GAGGTGGACA CCGCCTCCGT AATACCTATC 
ACGTCGATCC TGGAGTTCAA ACAGCGCTAC TACGGCGAGA AGCACGGCTT TGCTGGCGGC 
TGGGACGCGA ACAGCGAAGA CATGCAACGA CTGCGGGAGC ACTACGTGGA GGGGCTCATG 
TGGGTCATGC ATTACTACTA TCAAGGCGTG CCTTCATGGA AGTGGTTCTT TCCGCACTAC 
TACGCACCCA TGGCGAGCGA CATGGTGAAC CTCCCCGCTA TCGCTGCCCG CGTCTCGTTC 
GAACTCGGCA AGCCCTTCCA GCCGCACCAG CAGCTGCTGG CCGTGCTGCC GCCCATGTCA 
TACCTGTCCA TGCCGAAGGC CTACTGGCCC CTGCTGCGCA GCCCAAGTAG CCCGCTCGCC 
AAGTACTTGC CCGAGCACAT CGTTATTGAC CGCGAGGGCG CGCGCGCGCC GTGGGAGGGC 
ATTGTGCTGA TCCCCTTCAT CGACGAGCGC ACGCTGCTCG CCGCCTACGA GTCGGTGCAG 
AAGAACGTCA GCCCCGAGGA CGCCGCGAAC AACGTGAATG GCCCCCCGCT GCGGTTCTGC 
TACGACGGCA AGATGCCGCC GTACGACCTG GAGGATGAGA TGTTCGGCTC TATCCGGAAT 
GTCCTCGTGA GGCGCGAGAC ATACGCGTTC CCTCACATCG ACAAGTTCAT TCCACAGCTG 
TGCCCTGGCG TGCACGTTGG CACGCGGCAG CTGGAGGGCT TCAGCACCCT CCAGTCGAAG 
CGCGACCTAA TCCAGCCGGT CTTCGAGGCT GGGGTCGTGT CGATCTTTGG CCGTCCCACC 
AAGAACGACA GTCTCATCCT GCAGATGCGC GACTTCTTCA AGGCCAAGTC AGTCGACCAG 
GTGGCGACTC TCGTCGGCAA GGAGGTGCTC GTCGGCTTCC CGCACTACAA GCGTGCCCGC 
ATCGCCTCCC TCAAGGATGT GCACAAGTCC ATCACTGCTT CGTACAGCAA GGAGGGCACC 
TTCAGCGGCA CCGAGGAGCA CAAGAACAAC CGCGAAGAGT CGACTGCCTT CGTGAAGGAG 
GCGGACACGC ACGCGCGCTT CATGAAGTCG CAGCTGGGCC TCGCCGTGGA GCAGGTCGAA 
GTTCTCGTGT ACGTGCACCG CTTCAACGGA ATGCAGGTGA CGCGCAAGGG GCACATTGAG 
CGACACTTCG CCACCACCTG GACGTGCTAC CCAATCCAGC TGATCTGCAC CTTGGATAAC 
ATAGACATGC AGGAGGACAG CCGTTACGTC GAGCGCGACC GCAGCGAAGG TGACAGCGCC 
TTTGGTGCCC GCTGCGTTTA CATCGGTCCT CAGCCCAAGT CGAAGAAGTC GCAGATGGAC 
GTGTACGGCA GCTGTGGCAC GGTTGTGACG GCTGGCCAGC ACGACACGAC GACGCAGCAG 
GGCGATCAGA TGACGGTTCG CCTCAAGGTG TTTCAGGAGC CGCTGCGGCT GCCGGCGGAG 
CTGCTGCAGT TTGCCGCACA GCGCAACTGG ACGGCGCTGC CGCAGGTGGC GATCAGTCTC 
GACATTTTAC CGCACACCTT GACTATCATC TGTAGCTCCA TCGTCACGTC GCCGCAGTTT 
GGCTCTCGCG AGCTGGGGCT GTGTCTAAAG TTCACTGGGC GGTGCATCGC CAAGGTCGGG 
TACGCGAAGC TGGTCCAGCA CAGTCTGAAC CCGTGGTACG TTGGGAACAA GGACATCTTT 
GCCCGCATGG AAAAGGATAC GGAGGACATT GGCTACCACT TGGAGATGGG CGAGAAGGGC 
GACTTCACGG GCAATTCGAG CAGCAACCGT GGTGGCAGCG GCACGTGGTA CTTCTCCCGC 
GACGCGCAGG AGCTGATCCG TGACTACGTG AAGCGGTTCC GCCCACTCAT CCAGTACATC 
GAGGCGGGCG GCGTAAACTC GAACAGCGTT GAGCCGCAGC AGTTCCTGAC GGGCAAGTGG 
CGCGACAGGG ATGTCGACGA CGTGCTAACG GAGATCGAGA CATTCTTAAT CGAGTCGGGG 
CTGCGCGAAA CGCCGATGAT CACGGCCACG GAGGAGGCGT TTCCGCAGCA GCTGCTCGAG 
CAGCTCGAGG CGTCGCTGCA GGAGCAGCAG AACCGCAGCT TCAAGGAGCT CTCGTTGCGG 
CAGGTAAGCC GCAATCAGCT CTACTTTCCT GTCACGCGCA GCAGCTGCGG CCATCTCGTG 
CCGCTACCAC TGCCGCAGGA GCAGACTTTC TACCTGGGCT CGCGGGTCGC GAACTGCCGC 
GCTACGGGCA TTGTTCCCTT CGGCACGCAA GGCACGATCG TGCGCTTGCT GGCCAGCGGC 
AGGGAAGCAG AGGTCGTCTA CGATACCCCC TTCACGGGAG GCACGCGACT CGATGGCCGC 
CTCAAGGACA CTCGCGCCGC CATCACGAAG CTGTCGGCGC TGCTTGTACT CAAGTCGACG 
GATGCGCCGC AGCCACAGCT GCAACCTCAG ACGCAGCCGG ACATGAGGGC CGCTGCAGCC 
GCCACCACGA ACGGGTCGGC CAAGCTGCGG GAGCTGGAAT TCCTCCTGCA GGCGAACGGC 
ATTCACGTGG ACCCCTCGGG CAAGTCCACA GTGTCGCCGC CATCGCTGCC TGCAGCGTTT 
CAGAGCCCCA CAAGTGCCGC GGCTCGCGCT GCGCCGGCCG ACTCTGGTGC GAGCCATAGC 
AGCACCGCAG GCGCCGGTGC AAGCGTTGTG CCGCGGCCGC GCAGTGCCGC TGCGACGGAG 
CAGACGCCGG CACCAGCGAC ACTAGGCAAG CGCGCCACCC CTGCCTCCGC CACCGCCCAC 
TTGTCCTCAA CGTCTTCGGT GACGCCAATC ACCAGAGAGA CCCACAGCAG TCCCGCGAGC 
CTCAAAATCA CGGCTGCCCC AGGGAGCAGC GGGATCAGCC TTCAAGACTT GGCCAAACAC 
TTGCCGGCCT CCACATCCCA GCGCTCGCCC GCCACTGTCG CCTCGCCGGC TGGCCACGCT 
CCAGCGTCGG GTGCAGTTGC GGCAACAACG GCGGCGGCGC TCAGCGGCAT CACCATCCCG 
GCCCCCAAGT CTGCGCAGCA GCACCAACAC CCGATGCAGC CCGCTTCGCC GCCGGCGACG 
ACAGCTGTGG CCTCGCCAAG CCAAAGCAAT GAGTCGAACG AGACTTCCCC CGGCTCAAAC 
ACGCGCCGCA AGTCGCGCGA CCTGCACGTC ATCCCAGACG ACTTCGTCAG TGGACGGTTC 
AAGATCAAGT ACACGAACAC GGGCTCGCTG TTCCGCAGCT GGCTGGACAC CTTCACGGCG 
GAGGCGGTGC GCAAGACCCT CGCCGAGCTG GAGGCGGAGG GGAATGCGAA TCCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_06_0260 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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