Dpse\GA23522 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA23522
Entrez Gene ID	6900553
Full Name	GA23522 gene product from transcript GA23522-RB
Synonyms	GA23522,dpse_GLEANR_12440
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_002134900.2	XP_002134936.1	uncharacterized protein, isoform A
XM_015187430.1	XP_015042916.1	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA23522 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA23522 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe12613	XM_002134900.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA23522), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODe26267	XM_015187430.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA23522), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA23522 ( XM_002134900.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_002134936.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA23522), mRNA.

Price & Availability↑

CloneID	ODe12613
Clone ID Related Accession (Same CDS sequence)	XM_002134900.2
Accession Version	XM_002134900.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). On Dec 22, 2015 this sequence version replaced XM_002134900.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCGCC ACAGGATTGT TCGGACGATG GATTACAAAG ACGAATACGA TGGTTACGAT 
GACGTCTACG GACACTCCGT TGATGATGAC CACTGCATTT CACCCACAGA TGCCCAACAA 
TGGCTGTACG ACCGGGAGCG CGGCAGGCAG AGCATCTCCA CCTTCATTAG CAAGAACAAA 
GACATCAAGG AGGAAGGAGA GCACGACGAG GACGCCGAGG ACGAGGCCTA CGAGAAAGCC 
AGACGCGACT CGGAGAGCTT CCAGATGCCC GAACTGAACG AAATGGACCA GGCCAAGCTG 
AGTTCCTGCG TGGACGAGGT GCGGAGTGTG GTGGGCGACA CCGTGTCCGA GCGCCGCATT 
GTGGAGACCT CCATGAAATT TGACTACGAC ATACAGAAGA TTTTGGACGA GATCCTAAAC 
GGAGAGACAA AGAAGTCGGC CAAACCTACG CTCCGACCGA AGGTGCCTGT CCCCATTGCA 
GCCACTACAG TGAAGTCTGC CAGCAAGCCC CCGCCCCCGG CAAAAATCAG CCTAAAGGAG 
CCGCGGCGTG GCTTTGAAAT AGCTTCTCCC AAGTTACCGT CCTCCCCTGT AGTCTCTGGC 
AGGAATTCCC CCGTCGAGGT GGGAGAAGGC GATTTGTCCC GCACTTCCGC CAGCCTCTTT 
AAGGTCTCTA AGGACCAGTC CCAACGCAAT GCCCAGCAAC TGTACGAGAA GGAGCGATCC 
GACCAAAAGA GCCACATCCA CATGATCGTC ATCGGCCATG TAGATGCGGG AAAGAGCACC 
CTGATGGGCC ACTTACTCTA CGACACAGGA AACGTCTCGC AGCGCGTGAT GCACAAGCAC 
GAGCAAGAGT CCAAGAAGCT TGGGAAGCAG AGCTTCATGT ACGCCTGGGT TCTGGACGAG 
ACCGGCGAGG AGCGTGCCCG AGGTATCACC ATGGACGTGG GGCAGTCTCG CATCGAGACA 
AAGACAAAGA TTGTGACTCT GCTAGATGCG CCTGGCCACA AGGATTTCAT ACCCAACATG 
ATCTCCGGTG CCACACAGGC GGATGTGGCC CTCCTAGTGG TGGATGCCAC GCGGGGCGAA 
TTCGAATCCG GCTTTGAGCT GGGTGGACAG ACGCGCGAGC ATGCCATCCT GGTACGATCC 
CTGGGTGTAA ACCAGCTGGG CGTGGTTATC AACAAGCTGG ACACTGTTGG CTGGTCCCAG 
CAGCGATTCA CGGAGATTGT CACCAAACTA AAGTCCTTCC TTAAACAGGC GGGCTTCAAG 
GAGTCGGACG TAAGCTTCAC GCCCTGCTCT GGCCTGACCG GCGAGAATCT CACCAAGACA 
GCCCAGGAGA CCGCACTCAC CAGCTGGTAC AGTGGTCGCC ACCTGCTGGA CGTGATTGAG 
AATTTCAAGA TACCGGAGCG GGCCATCGAC AGACCGCTGC GGATGTCAGT CTCGGATATC 
TACAAGGGCA CTGGTTCTGG ATTCTGCATT TCTGGACGAG TTGAGACTGG TATGATCTGC 
GTGAATGATA AGGTGCTGGT CGGCGCAAGC AGGGAGCAGG CGCAGGTCAA GTCCCTGACC 
ATGGATGAGT TCACCCAGAC GAGCGTCTTT GCTGGCGATC AGATTTCGGT GACGCTGGCC 
GGCGTGGATA TCAACAACAT AACCGTCGGC TGCATCATCA GTGACCCACA GACACCTATT 
CCCGTGACCA CACGCTTCCA GGCCCGCATC ATCGTCTTCA GTGTCAAGGT GCCCATCACC 
ATGGGGTTTC CGGTTCTGCT CCACCACCAG TCGCTCATCG AGCCGGCTGT TGTTTGCAAA 
CTGACGGCAT CCATACACAA GAGCACCGGC GAGGTGGTAA AGAAGAAGCC GCGTTGCCTA 
GGCAGCAACT CGTGTGCCCT GGTCGAATTG GAGACGAGTC GCCCCATCTG CATAGAGCGG 
TACGCCGACT TCAAGGAACT GGGTCGCATC ATGTTGAGGG TGGCTGGTGT AACGATTGCT 
GCTGGAATGG TCACAAAGAT ACGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002134936.1
CDS	116..2122
Translation	MSRHRIVRTMDYKDEYDGYDDVYGHSVDDDHCISPTDAQQWLYDRERGRQSISTFISKNKDIKEEGEHDEDAEDEAYEKARRDSESFQMPELNEMDQAKLSSCVDEVRSVVGDTVSERRIVETSMKFDYDIQKILDEILNGETKKSAKPTLRPKVPVPIAATTVKSASKPPPPAKISLKEPRRGFEIASPKLPSSPVVSGRNSPVEVGEGDLSRTSASLFKVSKDQSQRNAQQLYEKERSDQKSHIHMIVIGHVDAGKSTLMGHLLYDTGNVSQRVMHKHEQESKKLGKQSFMYAWVLDETGEERARGITMDVGQSRIETKTKIVTLLDAPGHKDFIPNMISGATQADVALLVVDATRGEFESGFELGGQTREHAILVRSLGVNQLGVVINKLDTVGWSQQRFTEIVTKLKSFLKQAGFKESDVSFTPCSGLTGENLTKTAQETALTSWYSGRHLLDVIENFKIPERAIDRPLRMSVSDIYKGTGSGFCISGRVETGMICVNDKVLVGASREQAQVKSLTMDEFTQTSVFAGDQISVTLAGVDINNITVGCIISDPQTPIPVTTRFQARIIVFSVKVPITMGFPVLLHHQSLIEPAVVCKLTASIHKSTGEVVKKKPRCLGSNSCALVELETSRPICIERYADFKELGRIMLRVAGVTIAAGMVTKIR

Target ORF information:

RefSeq Version	XM_002134900.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA23522), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002134900.2

ORF Insert Sequence:

ATGTCGCGCC ACAGGATTGT TCGGACGATG GATTACAAAG ACGAATACGA TGGTTACGAT 
GACGTCTACG GACACTCCGT TGATGATGAC CACTGCATTT CACCCACAGA TGCCCAACAA 
TGGCTGTACG ACCGGGAGCG CGGCAGGCAG AGCATCTCCA CCTTCATTAG CAAGAACAAA 
GACATCAAGG AGGAAGGAGA GCACGACGAG GACGCCGAGG ACGAGGCCTA CGAGAAAGCC 
AGACGCGACT CGGAGAGCTT CCAGATGCCC GAACTGAACG AAATGGACCA GGCCAAGCTG 
AGTTCCTGCG TGGACGAGGT GCGGAGTGTG GTGGGCGACA CCGTGTCCGA GCGCCGCATT 
GTGGAGACCT CCATGAAATT TGACTACGAC ATACAGAAGA TTTTGGACGA GATCCTAAAC 
GGAGAGACAA AGAAGTCGGC CAAACCTACG CTCCGACCGA AGGTGCCTGT CCCCATTGCA 
GCCACTACAG TGAAGTCTGC CAGCAAGCCC CCGCCCCCGG CAAAAATCAG CCTAAAGGAG 
CCGCGGCGTG GCTTTGAAAT AGCTTCTCCC AAGTTACCGT CCTCCCCTGT AGTCTCTGGC 
AGGAATTCCC CCGTCGAGGT GGGAGAAGGC GATTTGTCCC GCACTTCCGC CAGCCTCTTT 
AAGGTCTCTA AGGACCAGTC CCAACGCAAT GCCCAGCAAC TGTACGAGAA GGAGCGATCC 
GACCAAAAGA GCCACATCCA CATGATCGTC ATCGGCCATG TAGATGCGGG AAAGAGCACC 
CTGATGGGCC ACTTACTCTA CGACACAGGA AACGTCTCGC AGCGCGTGAT GCACAAGCAC 
GAGCAAGAGT CCAAGAAGCT TGGGAAGCAG AGCTTCATGT ACGCCTGGGT TCTGGACGAG 
ACCGGCGAGG AGCGTGCCCG AGGTATCACC ATGGACGTGG GGCAGTCTCG CATCGAGACA 
AAGACAAAGA TTGTGACTCT GCTAGATGCG CCTGGCCACA AGGATTTCAT ACCCAACATG 
ATCTCCGGTG CCACACAGGC GGATGTGGCC CTCCTAGTGG TGGATGCCAC GCGGGGCGAA 
TTCGAATCCG GCTTTGAGCT GGGTGGACAG ACGCGCGAGC ATGCCATCCT GGTACGATCC 
CTGGGTGTAA ACCAGCTGGG CGTGGTTATC AACAAGCTGG ACACTGTTGG CTGGTCCCAG 
CAGCGATTCA CGGAGATTGT CACCAAACTA AAGTCCTTCC TTAAACAGGC GGGCTTCAAG 
GAGTCGGACG TAAGCTTCAC GCCCTGCTCT GGCCTGACCG GCGAGAATCT CACCAAGACA 
GCCCAGGAGA CCGCACTCAC CAGCTGGTAC AGTGGTCGCC ACCTGCTGGA CGTGATTGAG 
AATTTCAAGA TACCGGAGCG GGCCATCGAC AGACCGCTGC GGATGTCAGT CTCGGATATC 
TACAAGGGCA CTGGTTCTGG ATTCTGCATT TCTGGACGAG TTGAGACTGG TATGATCTGC 
GTGAATGATA AGGTGCTGGT CGGCGCAAGC AGGGAGCAGG CGCAGGTCAA GTCCCTGACC 
ATGGATGAGT TCACCCAGAC GAGCGTCTTT GCTGGCGATC AGATTTCGGT GACGCTGGCC 
GGCGTGGATA TCAACAACAT AACCGTCGGC TGCATCATCA GTGACCCACA GACACCTATT 
CCCGTGACCA CACGCTTCCA GGCCCGCATC ATCGTCTTCA GTGTCAAGGT GCCCATCACC 
ATGGGGTTTC CGGTTCTGCT CCACCACCAG TCGCTCATCG AGCCGGCTGT TGTTTGCAAA 
CTGACGGCAT CCATACACAA GAGCACCGGC GAGGTGGTAA AGAAGAAGCC GCGTTGCCTA 
GGCAGCAACT CGTGTGCCCT GGTCGAATTG GAGACGAGTC GCCCCATCTG CATAGAGCGG 
TACGCCGACT TCAAGGAACT GGGTCGCATC ATGTTGAGGG TGGCTGGTGT AACGATTGCT 
GCTGGAATGG TCACAAAGAT ACGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA23522 ( XM_015187430.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015042916.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA23522), mRNA.

Price & Availability↑

CloneID	ODe26267
Clone ID Related Accession (Same CDS sequence)	XM_015187430.1
Accession Version	XM_015187430.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCGCC ACAGGATTGT TCGGACGATG GATTACAAAG ACGAATACGA TGATGCCCAA 
CAATGGCTGT ACGACCGGGA GCGCGGCAGG CAGAGCATCT CCACCTTCAT TAGCAAGAAC 
AAAGACATCA AGGAGGAAGG AGAGCACGAC GAGGACGCCG AGGACGAGGC CTACGAGAAA 
GCCAGACGCG ACTCGGAGAG CTTCCAGATG CCCGAACTGA ACGAAATGGA CCAGGCCAAG 
CTGAGTTCCT GCGTGGACGA GGTGCGGAGT GTGGTGGGCG ACACCGTGTC CGAGCGCCGC 
ATTGTGGAGA CCTCCATGAA ATTTGACTAC GACATACAGA AGATTTTGGA CGAGATCCTA 
AACGGAGAGA CAAAGAAGTC GGCCAAACCT ACGCTCCGAC CGAAGGTGCC TGTCCCCATT 
GCAGCCACTA CAGTGAAGTC TGCCAGCAAG CCCCCGCCCC CGGCAAAAAT CAGCCTAAAG 
GAGCCGCGGC GTGGCTTTGA AATAGCTTCT CCCAAGTTAC CGTCCTCCCC TGTAGTCTCT 
GGCAGGAATT CCCCCGTCGA GGTGGGAGAA GGCGATTTGT CCCGCACTTC CGCCAGCCTC 
TTTAAGGTCT CTAAGGACCA GTCCCAACGC AATGCCCAGC AACTGTACGA GAAGGAGCGA 
TCCGACCAAA AGAGCCACAT CCACATGATC GTCATCGGCC ATGTAGATGC GGGAAAGAGC 
ACCCTGATGG GCCACTTACT CTACGACACA GGAAACGTCT CGCAGCGCGT GATGCACAAG 
CACGAGCAAG AGTCCAAGAA GCTTGGGAAG CAGAGCTTCA TGTACGCCTG GGTTCTGGAC 
GAGACCGGCG AGGAGCGTGC CCGAGGTATC ACCATGGACG TGGGGCAGTC TCGCATCGAG 
ACAAAGACAA AGATTGTGAC TCTGCTAGAT GCGCCTGGCC ACAAGGATTT CATACCCAAC 
ATGATCTCCG GTGCCACACA GGCGGATGTG GCCCTCCTAG TGGTGGATGC CACGCGGGGC 
GAATTCGAAT CCGGCTTTGA GCTGGGTGGA CAGACGCGCG AGCATGCCAT CCTGGTACGA 
TCCCTGGGTG TAAACCAGCT GGGCGTGGTT ATCAACAAGC TGGACACTGT TGGCTGGTCC 
CAGCAGCGAT TCACGGAGAT TGTCACCAAA CTAAAGTCCT TCCTTAAACA GGCGGGCTTC 
AAGGAGTCGG ACGTAAGCTT CACGCCCTGC TCTGGCCTGA CCGGCGAGAA TCTCACCAAG 
ACAGCCCAGG AGACCGCACT CACCAGCTGG TACAGTGGTC GCCACCTGCT GGACGTGATT 
GAGAATTTCA AGATACCGGA GCGGGCCATC GACAGACCGC TGCGGATGTC AGTCTCGGAT 
ATCTACAAGG GCACTGGTTC TGGATTCTGC ATTTCTGGAC GAGTTGAGAC TGGTATGATC 
TGCGTGAATG ATAAGGTGCT GGTCGGCGCA AGCAGGGAGC AGGCGCAGGT CAAGTCCCTG 
ACCATGGATG AGTTCACCCA GACGAGCGTC TTTGCTGGCG ATCAGATTTC GGTGACGCTG 
GCCGGCGTGG ATATCAACAA CATAACCGTC GGCTGCATCA TCAGTGACCC ACAGACACCT 
ATTCCCGTGA CCACACGCTT CCAGGCCCGC ATCATCGTCT TCAGTGTCAA GGTGCCCATC 
ACCATGGGGT TTCCGGTTCT GCTCCACCAC CAGTCGCTCA TCGAGCCGGC TGTTGTTTGC 
AAACTGACGG CATCCATACA CAAGAGCACC GGCGAGGTGG TAAAGAAGAA GCCGCGTTGC 
CTAGGCAGCA ACTCGTGTGC CCTGGTCGAA TTGGAGACGA GTCGCCCCAT CTGCATAGAG 
CGGTACGCCG ACTTCAAGGA ACTGGGTCGC ATCATGTTGA GGGTGGCTGG TGTAACGATT 
GCTGCTGGAA TGGTCACAAA GATACGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015042916.1
CDS	116..2065
Translation	MSRHRIVRTMDYKDEYDDAQQWLYDRERGRQSISTFISKNKDIKEEGEHDEDAEDEAYEKARRDSESFQMPELNEMDQAKLSSCVDEVRSVVGDTVSERRIVETSMKFDYDIQKILDEILNGETKKSAKPTLRPKVPVPIAATTVKSASKPPPPAKISLKEPRRGFEIASPKLPSSPVVSGRNSPVEVGEGDLSRTSASLFKVSKDQSQRNAQQLYEKERSDQKSHIHMIVIGHVDAGKSTLMGHLLYDTGNVSQRVMHKHEQESKKLGKQSFMYAWVLDETGEERARGITMDVGQSRIETKTKIVTLLDAPGHKDFIPNMISGATQADVALLVVDATRGEFESGFELGGQTREHAILVRSLGVNQLGVVINKLDTVGWSQQRFTEIVTKLKSFLKQAGFKESDVSFTPCSGLTGENLTKTAQETALTSWYSGRHLLDVIENFKIPERAIDRPLRMSVSDIYKGTGSGFCISGRVETGMICVNDKVLVGASREQAQVKSLTMDEFTQTSVFAGDQISVTLAGVDINNITVGCIISDPQTPIPVTTRFQARIIVFSVKVPITMGFPVLLHHQSLIEPAVVCKLTASIHKSTGEVVKKKPRCLGSNSCALVELETSRPICIERYADFKELGRIMLRVAGVTIAAGMVTKIR

Target ORF information:

RefSeq Version	XM_015187430.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA23522), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015187430.1

ORF Insert Sequence:

ATGTCGCGCC ACAGGATTGT TCGGACGATG GATTACAAAG ACGAATACGA TGATGCCCAA 
CAATGGCTGT ACGACCGGGA GCGCGGCAGG CAGAGCATCT CCACCTTCAT TAGCAAGAAC 
AAAGACATCA AGGAGGAAGG AGAGCACGAC GAGGACGCCG AGGACGAGGC CTACGAGAAA 
GCCAGACGCG ACTCGGAGAG CTTCCAGATG CCCGAACTGA ACGAAATGGA CCAGGCCAAG 
CTGAGTTCCT GCGTGGACGA GGTGCGGAGT GTGGTGGGCG ACACCGTGTC CGAGCGCCGC 
ATTGTGGAGA CCTCCATGAA ATTTGACTAC GACATACAGA AGATTTTGGA CGAGATCCTA 
AACGGAGAGA CAAAGAAGTC GGCCAAACCT ACGCTCCGAC CGAAGGTGCC TGTCCCCATT 
GCAGCCACTA CAGTGAAGTC TGCCAGCAAG CCCCCGCCCC CGGCAAAAAT CAGCCTAAAG 
GAGCCGCGGC GTGGCTTTGA AATAGCTTCT CCCAAGTTAC CGTCCTCCCC TGTAGTCTCT 
GGCAGGAATT CCCCCGTCGA GGTGGGAGAA GGCGATTTGT CCCGCACTTC CGCCAGCCTC 
TTTAAGGTCT CTAAGGACCA GTCCCAACGC AATGCCCAGC AACTGTACGA GAAGGAGCGA 
TCCGACCAAA AGAGCCACAT CCACATGATC GTCATCGGCC ATGTAGATGC GGGAAAGAGC 
ACCCTGATGG GCCACTTACT CTACGACACA GGAAACGTCT CGCAGCGCGT GATGCACAAG 
CACGAGCAAG AGTCCAAGAA GCTTGGGAAG CAGAGCTTCA TGTACGCCTG GGTTCTGGAC 
GAGACCGGCG AGGAGCGTGC CCGAGGTATC ACCATGGACG TGGGGCAGTC TCGCATCGAG 
ACAAAGACAA AGATTGTGAC TCTGCTAGAT GCGCCTGGCC ACAAGGATTT CATACCCAAC 
ATGATCTCCG GTGCCACACA GGCGGATGTG GCCCTCCTAG TGGTGGATGC CACGCGGGGC 
GAATTCGAAT CCGGCTTTGA GCTGGGTGGA CAGACGCGCG AGCATGCCAT CCTGGTACGA 
TCCCTGGGTG TAAACCAGCT GGGCGTGGTT ATCAACAAGC TGGACACTGT TGGCTGGTCC 
CAGCAGCGAT TCACGGAGAT TGTCACCAAA CTAAAGTCCT TCCTTAAACA GGCGGGCTTC 
AAGGAGTCGG ACGTAAGCTT CACGCCCTGC TCTGGCCTGA CCGGCGAGAA TCTCACCAAG 
ACAGCCCAGG AGACCGCACT CACCAGCTGG TACAGTGGTC GCCACCTGCT GGACGTGATT 
GAGAATTTCA AGATACCGGA GCGGGCCATC GACAGACCGC TGCGGATGTC AGTCTCGGAT 
ATCTACAAGG GCACTGGTTC TGGATTCTGC ATTTCTGGAC GAGTTGAGAC TGGTATGATC 
TGCGTGAATG ATAAGGTGCT GGTCGGCGCA AGCAGGGAGC AGGCGCAGGT CAAGTCCCTG 
ACCATGGATG AGTTCACCCA GACGAGCGTC TTTGCTGGCG ATCAGATTTC GGTGACGCTG 
GCCGGCGTGG ATATCAACAA CATAACCGTC GGCTGCATCA TCAGTGACCC ACAGACACCT 
ATTCCCGTGA CCACACGCTT CCAGGCCCGC ATCATCGTCT TCAGTGTCAA GGTGCCCATC 
ACCATGGGGT TTCCGGTTCT GCTCCACCAC CAGTCGCTCA TCGAGCCGGC TGTTGTTTGC 
AAACTGACGG CATCCATACA CAAGAGCACC GGCGAGGTGG TAAAGAAGAA GCCGCGTTGC 
CTAGGCAGCA ACTCGTGTGC CCTGGTCGAA TTGGAGACGA GTCGCCCCAT CTGCATAGAG 
CGGTACGCCG ACTTCAAGGA ACTGGGTCGC ATCATGTTGA GGGTGGCTGG TGTAACGATT 
GCTGCTGGAA TGGTCACAAA GATACGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA23522 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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