Dpse\GA30460 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA30460
Entrez Gene ID	6898777
Full Name	GA30460 gene product from transcript GA30460-RF
Synonyms	Dpse\GA12651,Dpse\GA24224,Dpse\GA24225,Dpse_GA12651,Dpse_GA12652,Dpse_GA21337,Dpse_GA24224,Dpse_GA24225,GA12651,GA12652,GA21337,GA24224,GA24225,GA30460,dpse_GLEANR_14025,dpse_GLEANR_14026,dpse_GLEANR_14027
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015184950.1	XP_015040436.1	uncharacterized protein, isoform E
XM_015184952.1	XP_015040438.1	uncharacterized protein, isoform G
XM_015184948.1	XP_015040434.1	uncharacterized protein, isoform C
XM_015184949.1	XP_015040435.1	uncharacterized protein, isoform D
XM_015184951.1	XP_015040437.1	uncharacterized protein, isoform F
XM_002138718.3	XP_002138754.2	uncharacterized protein, isoform B
XM_004444334.2	XP_004444391.1	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA30460 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA30460 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODe06605	XM_002138718.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA30460), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODe23356	XM_015184949.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant D (Dpse\GA30460), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODe06604	XM_004444334.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA30460), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODe23355	XM_015184948.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA30460), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODe23357	XM_015184950.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant E (Dpse\GA30460), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODe23357	XM_015184951.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant F (Dpse\GA30460), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODe23358	XM_015184952.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant G (Dpse\GA30460), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA30460 ( XM_002138718.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_002138754.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA30460), mRNA.

Price & Availability↑

CloneID	ODe06605
Clone ID Related Accession (Same CDS sequence)	XM_002138718.3
Accession Version	XM_002138718.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_002138718.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGGTT ATCGCTCGTT GCCGCTGGCC CCCGTTTCGG CCGGCAGTAT GGCAGCGGCG 
GCGGCCGCCG TTCCGGCCCA AAATGTGGGT CATGCCGGAC CCACGATGGC CTATGCCCAC 
CACCAGTCGA TGACGGCCAT GTCATCGAAT AACGGTATTA TCTATCAGTC GGCGGTGCAC 
GCCTCCATAC ACAGCCTCCC GAGCCAGCAT CGCTCATCCA CCACGCATCA TCACATTCTA 
GCCACGGCCA CCGCCACAGC AACGGCCGCA GCAGCAGCAG CGGCAGCCAC AGCCGCCGCA 
GCAATGGTGT CCCTGCCGGC GACAGCAGCA GCAGCAGCAC AGCAACACGG AACCTATGGA 
CACCAGCAGC AGCCACACCA CCCACAACAG CAGCAGCAGC AGCAACAACA GCAGCAGCAG 
CAGCATCTTC CTCAGCAATC CCATCAGCAG CAGCAGCAGC AGCAGCAGCA TCAGCAACAA 
CTGATGTCCC ATCAGCAGCA TTCCGCGTTG CAGCAGCAGC AGCAACAACA TCACCATCTA 
CAGCAATCCC ATCAGCAACA GCAACAGCTC CATCAGCAGC AACAGCAACA GCAGCAGCAG 
CAGCATCTCT CTGCCGTGCA CCACCACCAT CATCACCACC ACCAGCAGCT GTCGCTGCAC 
CAGAACCACT TGTACTTCAG CGGTGGGAGT CTGGGGCACC ATCACCACCA TCGACAGATC 
TTCAGCCACC TGCAGAGCGC CCTAATGCCG CTCAGTGTCA ATGGCGAACC TATCAAACTA 
CCCGACAATT TGGAGAGCCT GCCAAGAGCT GACAGTTTTC CATCACAGCG TCATCGTTGG 
AATACAAATG AGGAAATTGC TGCGATTCTT ATCAGCTTTG ACAAGCACTG CGAGTGGCAG 
TCCAAGGAGG TCCGAACCAG ACCCAAGAGC GGATCCCTGC TGCTGTACTC GCGGAAGAAG 
GTGCGCTATC GCCGCGACGG CTATTGCTGG AAGAAGCGCA AGGATGGCAA GACGACGCGC 
GAGGATCACA TGAAACTGAA AGTACAAGGC ACGGAGTGCA TCTACGGCTG TTATGTACAC 
TCGGCCATAT TGCCCACGTT CCATCGCAGA TGTTACTGGC TGTTGCAGAA TCCGGACATT 
GTTTTGGTGC ACTATCTGAA TGTGCCGTAT CCGGATGATA ATAAAATGGC AGTGATTGCC 
CCCAGCATCA CCCTGTGGGG CGACAAGAAA GAGTGGACCA AGGAGGAGCT GGTCAGCCAG 
CTGAAGCCGA TGCTCTCCAC AGTCAACTCC GATGATGACT CCGACTCGGG CAACGACATT 
GAGATATCGA CGGCCGAGAC CGTGGAGTCG ATTGTGTGCC AGCTGATGGA GAAGCAGCGC 
TTGTCCCGCC AGGCGGCCCT CGTCAAGCAG CTCGACTGCG GCTGCGGGGA CTCGGGCTGT 
GCCGATGGCA AGACCTGCAC GCATCCCGTG ATGCGGCGCA CGGGCGGCAT CATCAAGTCC 
GCGGTGGCCA CATCGGCCTC AGAGAAGCGA CTCAACGAGG GGGCCTTCAA CGGCGGCACG 
CCCAATGTGG TGGTGGCCAG CAAGCTATAC TCCCGCTGGG CGGAGCGGCC GCGCCAGCGC 
AACGATGGCA TGCACCTGGA CAACCCGCAC GCACAGTTCC ACAATGCCAC GCAACAGCTG 
CCCCAGGAAT CGGCCATCAA GTTTCAGATC ATTCCTCCTC AACAGCAGCA GCAGCAGCAA 
CAACAGACGG GACATGGCGA TGGCAGCTAT CAGCAGCAAT ATCGTGGTGC CAATCAGATG 
CTGGCCGCAA GAAGCAATCT GGTGATGCAG CAGCAACAGC AACAGCAGCA GCAATATCAC 
CAGCAACAGC AGCAACACTT GAGTCTGCAA CGATTCGTGG CCAGTCAGAG CCAGAATTCG 
GCTCATTTGA ATCATCATCA TCATCGCCTG CAAATTGCCA ATACAACAAT CACGGCCACA 
GCCAGAGATC CTTCCACAAC AGTAGCAGCA GCAGCAACAG CAGCATCGGC ATCAGCAGCA 
GGAGGAGGCT CCGTCTTAAA TGGAATGGAT ACGGAACTAA TTGAAATCCA AAGCCACATG 
ACTGCAAACA AAATCACTAG CTCCGTCAGC AGCAATGGAA ATGGCAGCGG CAGCAATCCC 
AGTGCCAGTG CCAAGCAATT AGCAGCTCAA ACGAACAACG AATTAAATGT CGTAAATAAT 
AACGACCCCA GCAACAATAA CTTTATGTAC AATCAACAAC AGCAGCAACT GAATACTTCC 
AGCAACAGCA ACAACACACA GCAGCAGCAG CAACATTATT ATAAATTGCA ACAGACATCA 
GCAACCCCAA ACAGTGGCCA ACTCCAACTC CAAAATCAAA CCCAAACACA AACACAAACC 
CCAACCCTTG TCCAACCCCC TGTTGAGGCC ATGTGTATGT CACCTGAGCA TCGCAGCGGC 
AGCAGCAGCA GCAGCAACAA CAATACGGCC TCAACGTCTT CCGCTGTCAA CAGTCATAAC 
ACCTCCCCCT CCACAGCCAT AGCCACAACC ACGTCCATGG CCGCATCTAT ATCTATATCC 
ACATCTACGT CCGGCACATC GTCCACCTCG AGTTCCTTAC AATCGATTAT CGATGGCAAT 
CAGCAGCAAC AAATTACAAC GACATCGACG GCAGCAACTT GTGATAATTT AATGAGCGCC 
AATGCGCTCA CTGAGCTGAA TGGAGGGAGC AGCAGCAGCA GCAACAATCA GGCCACAGAT 
GGCCACAACA AGAGTCAATC CCAATCGCAG TCTCAGCCGC AAACTCTGAG CCAAAATGGT 
TGTGCAACAT TCAGTGCTTC CAGTGATAAT AGCAGCCAAA TTAGCGCCAG CGACGAGAGT 
AGCAGCTTTG GGGCCAACAA TCAGAACAAC AGCATACACA GCATCCACAG CAGCCACAGC 
AGCAGCAGTA ACAGCAGCGA GGACGAGCCC CAGGCGGGCA ATGCTGCCGA GACCCTGAGC 
TTCTTCAACG AAACCCTGGA TCTATCCCAT GAGGATATTC AACGTACACT GATAGCCAAT 
ATGCAGCCGT ACAATACGGC TGCAGGCGAA GCAGCAGCGA CAACGATAGA ACCTTTGGCA 
ACAAACGGCA GCCACAGTAG CAGCAACAGC AATGGCAATC CTCAAGAAGT GGAGAAGAAA 
CCTTCAGTCG GCCAGGAAGT CGAAGCCGAA CCTGAGGAGG ATGACACAGA CGATGTGTTT 
GCCAACCTGG ATGCCTTCGA TATGCTCGTG GAGTTCCCCG AGCTGGATCT GGATGATAAG 
CAGGCCCTGA ACAACACGGC CTTGGAGCAG GGCTCCTATC TGGGACAGGC GGCCGCCCAA 
ACGCAGCAGC CCCGCAAGAT CCACAACATC TGCGACTTCA GTCCGGAGTG GTCGTACACC 
GAGGGCGGAG TCAAGGTACT TGTGGCCGGA CCCTGGACGA GCAGCAATGG TGCTGGTGCC 
TACACGGTGC TCTTCGATGC CCAGCCCGTG CCCACGCAGA TGGTGCAGGA GGGAGTGCTC 
CGTTGCTACT GTCCTGCCCA CGAGGCGGGC TTTGTGACGC TCCAGGTGGC CTGCGGCGGC 
TTTCTCGTGT CCAATTCGGT CATGTTCGAG TACAAGCTGT CGCTGCTGGC CGATGCCCCG 
TTCGATGCGA GCAGCTCCAA CGATTGCCTG TACAAATTCA CGCTGCTCAA TCGCCTCTCG 
ACCATCGACG ACAAGTTGCA GCTGAAGACG GAGCAGGAGC CGACGACGGA CCACACGGCT 
CTCTATCTGG AGCCGAACTT TGAGGAGAAG CTGGTCGCGT ACTGCCACAG GCTGACGAAG 
CATGCCTGGA GCATGCCCAG CACAGTGGCC AGCTGGAGCG TGGGCCTGCG GGGCATGACG 
CTGCTGCATC TGGCCGCCGC CTTGGGCTAT GCCAAGCTGG TAGGCGCCAT GCTCAATTGG 
CGGGCGGAGA ATCCACATAT CATACTCGAA ACGGAGCTGG ACGCCCTCAG TCAGGATGTG 
TACGGTTTCA CGCCGCTCGC CTGGTCCTGT GTGCGCGGCC ATGTGGAGTG TTCCCTGCTG 
CTCTACAAGT GGAATCACAA TGCGCTGAAG ATCAAGACGC AGAGCCAGCA AACGGCCCTG 
GATTTGGCCA ACCTGAAGGG GCACAAACAT CTCCTGTCCC AGATATTTCG ACTGGAGAAG 
GAGCGCTGCC GCAAGCCGCA CTTGCGAGGC GGCCTGGCCA ATCTTTCAAT GAACCTAGGC 
GTTGAAACGG AAGCGGAGGA GTCGCCGCCG CAGACCTACA ACAGCTTTGA CCTGGAGATA 
CTCCAAAGAA AGCACGATGG CGTCTTCCTC AGACCTGGGG CAGTGCATAG CAATCAAAGT 
CCTCCCAACA ATGGCAGCCG GTACTCCAAG CGCTCTTCCA TAGACAGCGG CATCAACATG 
GACATACGCA GCAAGTCAGG CAAGTCCCTG GCCAGGCTGC ACAGCAACTT TGAGGGGCAC 
GACAGCTATG CATTGGGAGT GGACTCGCCG CTGGATACGC TGACTGGGAC CAATTGCCTG 
CTCTCACCGC TACGTAAAAT GGATTTCGCC CTGTGGGACT CGGATGCCAA AGTGCTGACT 
CTGGCCGAAC ACATTATAGC AGCAATGCCA GAGCGCATCA AAAATGAAGC CGATGAAATG 
ATGGTGCTGG GCAGCCCCTT GACTGAGCCA CTGACCTCGG AGTCCTCGGC CCTGACAGAC 
AGCTTTATGG ATCCCCTGCT GGACTCGCTG CCCAATACGC ATTTCGACAG CGACTTCAAC 
TTCGACTTCC ACGACCACAG CTATCGGTAC CACGATGTGA GCACTCCCTG CTCCAGCCTG 
AGTCCCGCCA GCTCGGGGCC GCTGCAGTCG CCGGCCAGCT ACTCCATACT GGGCACAGAT 
CCGTCTGTCA GCTCGCCCAG TCCGCCGCCG TCGACCAAGC AGCTGACCGA GTTCCTGCAC 
GCATCCAGCC TGTCGCAGTA TCCGTTCGAG GCGGACTTCT CCAAGCTGAC GCTGACCGAC 
ACGGAGCAGC GGGAGCTGTA CGAGGCGGCC AAGTGCATAC AGAAGGCCTA CCGCTCGTAC 
AAGGGCCGGC AGAAGCTCGA GGAGCAGAAC AAAGAGCGCA CTGCCGCCAT TGTCATCCAG 
AACTATTACA GGCGGTACAA GCAGTACGCC TATTACAGGC AGATGACCAA CGCCGCCCTG 
GTCATCCAGC ATGGCTATCG TTCCTATCGA AAGAACAAAC GCTTCAAGAA GAGCGGCCTG 
GGCCAGGGCA TGGCAGGGGG CAGCGATCAC GGCAGCGTCA GCAGCAACTC TCAGTGCCTG 
TCCAGCTTCT ACGACCACTA CAAGCAGGAT CAGCAGCAGC TGCAGCAGCA GCATGAGATG 
GGCTCGCAGC CGAGCACGCC CAAGGAAACC AGTCCCTCGG GTCCCTTGAA ACGAACCTAC 
TCGCAGTCCA CCCAGAATCA AGCTGCCAGG AAGATCCAAC AGTTCATGAG ACAATCACGC 
ATCAAACTAC AGAGAGAGCG AGCCGAAAAA GAGAAGCTGG TGCACCAACG CAGGGCGGAA 
TACCTTCAAA ACTTGCAGTA TCAAGGGCAG CAGGAAATCT TGGCTTGCCT AGAAAATAAC 
AGTTCTGCCC AGAGCAGTGG CAGCAACACC TATACGAACA GCATCAACAA TCTACAATCC 
AATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002138754.2
CDS	218..5926
Translation	MAGYRSLPLAPVSAGSMAAAAAAVPAQNVGHAGPTMAYAHHQSMTAMSSNNGIIYQSAVHASIHSLPSQHRSSTTHHHILATATATATAAAAAAAATAAAAMVSLPATAAAAAQQHGTYGHQQQPHHPQQQQQQQQQQQQQHLPQQSHQQQQQQQQHQQQLMSHQQHSALQQQQQQHHHLQQSHQQQQQLHQQQQQQQQQQHLSAVHHHHHHHHQQLSLHQNHLYFSGGSLGHHHHHRQIFSHLQSALMPLSVNGEPIKLPDNLESLPRADSFPSQRHRWNTNEEIAAILISFDKHCEWQSKEVRTRPKSGSLLLYSRKKVRYRRDGYCWKKRKDGKTTREDHMKLKVQGTECIYGCYVHSAILPTFHRRCYWLLQNPDIVLVHYLNVPYPDDNKMAVIAPSITLWGDKKEWTKEELVSQLKPMLSTVNSDDDSDSGNDIEISTAETVESIVCQLMEKQRLSRQAALVKQLDCGCGDSGCADGKTCTHPVMRRTGGIIKSAVATSASEKRLNEGAFNGGTPNVVVASKLYSRWAERPRQRNDGMHLDNPHAQFHNATQQLPQESAIKFQIIPPQQQQQQQQQTGHGDGSYQQQYRGANQMLAARSNLVMQQQQQQQQQYHQQQQQHLSLQRFVASQSQNSAHLNHHHHRLQIANTTITATARDPSTTVAAAATAASASAAGGGSVLNGMDTELIEIQSHMTANKITSSVSSNGNGSGSNPSASAKQLAAQTNNELNVVNNNDPSNNNFMYNQQQQQLNTSSNSNNTQQQQQHYYKLQQTSATPNSGQLQLQNQTQTQTQTPTLVQPPVEAMCMSPEHRSGSSSSSNNNTASTSSAVNSHNTSPSTAIATTTSMAASISISTSTSGTSSTSSSLQSIIDGNQQQQITTTSTAATCDNLMSANALTELNGGSSSSSNNQATDGHNKSQSQSQSQPQTLSQNGCATFSASSDNSSQISASDESSSFGANNQNNSIHSIHSSHSSSSNSSEDEPQAGNAAETLSFFNETLDLSHEDIQRTLIANMQPYNTAAGEAAATTIEPLATNGSHSSSNSNGNPQEVEKKPSVGQEVEAEPEEDDTDDVFANLDAFDMLVEFPELDLDDKQALNNTALEQGSYLGQAAAQTQQPRKIHNICDFSPEWSYTEGGVKVLVAGPWTSSNGAGAYTVLFDAQPVPTQMVQEGVLRCYCPAHEAGFVTLQVACGGFLVSNSVMFEYKLSLLADAPFDASSSNDCLYKFTLLNRLSTIDDKLQLKTEQEPTTDHTALYLEPNFEEKLVAYCHRLTKHAWSMPSTVASWSVGLRGMTLLHLAAALGYAKLVGAMLNWRAENPHIILETELDALSQDVYGFTPLAWSCVRGHVECSLLLYKWNHNALKIKTQSQQTALDLANLKGHKHLLSQIFRLEKERCRKPHLRGGLANLSMNLGVETEAEESPPQTYNSFDLEILQRKHDGVFLRPGAVHSNQSPPNNGSRYSKRSSIDSGINMDIRSKSGKSLARLHSNFEGHDSYALGVDSPLDTLTGTNCLLSPLRKMDFALWDSDAKVLTLAEHIIAAMPERIKNEADEMMVLGSPLTEPLTSESSALTDSFMDPLLDSLPNTHFDSDFNFDFHDHSYRYHDVSTPCSSLSPASSGPLQSPASYSILGTDPSVSSPSPPPSTKQLTEFLHASSLSQYPFEADFSKLTLTDTEQRELYEAAKCIQKAYRSYKGRQKLEEQNKERTAAIVIQNYYRRYKQYAYYRQMTNAALVIQHGYRSYRKNKRFKKSGLGQGMAGGSDHGSVSSNSQCLSSFYDHYKQDQQQLQQQHEMGSQPSTPKETSPSGPLKRTYSQSTQNQAARKIQQFMRQSRIKLQRERAEKEKLVHQRRAEYLQNLQYQGQQEILACLENNSSAQSSGSNTYTNSINNLQSNQ

Target ORF information:

RefSeq Version	XM_002138718.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA30460), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002138718.3

ORF Insert Sequence:

ATGGCCGGTT ATCGCTCGTT GCCGCTGGCC CCCGTTTCGG CCGGCAGTAT GGCAGCGGCG 
GCGGCCGCCG TTCCGGCCCA AAATGTGGGT CATGCCGGAC CCACGATGGC CTATGCCCAC 
CACCAGTCGA TGACGGCCAT GTCATCGAAT AACGGTATTA TCTATCAGTC GGCGGTGCAC 
GCCTCCATAC ACAGCCTCCC GAGCCAGCAT CGCTCATCCA CCACGCATCA TCACATTCTA 
GCCACGGCCA CCGCCACAGC AACGGCCGCA GCAGCAGCAG CGGCAGCCAC AGCCGCCGCA 
GCAATGGTGT CCCTGCCGGC GACAGCAGCA GCAGCAGCAC AGCAACACGG AACCTATGGA 
CACCAGCAGC AGCCACACCA CCCACAACAG CAGCAGCAGC AGCAACAACA GCAGCAGCAG 
CAGCATCTTC CTCAGCAATC CCATCAGCAG CAGCAGCAGC AGCAGCAGCA TCAGCAACAA 
CTGATGTCCC ATCAGCAGCA TTCCGCGTTG CAGCAGCAGC AGCAACAACA TCACCATCTA 
CAGCAATCCC ATCAGCAACA GCAACAGCTC CATCAGCAGC AACAGCAACA GCAGCAGCAG 
CAGCATCTCT CTGCCGTGCA CCACCACCAT CATCACCACC ACCAGCAGCT GTCGCTGCAC 
CAGAACCACT TGTACTTCAG CGGTGGGAGT CTGGGGCACC ATCACCACCA TCGACAGATC 
TTCAGCCACC TGCAGAGCGC CCTAATGCCG CTCAGTGTCA ATGGCGAACC TATCAAACTA 
CCCGACAATT TGGAGAGCCT GCCAAGAGCT GACAGTTTTC CATCACAGCG TCATCGTTGG 
AATACAAATG AGGAAATTGC TGCGATTCTT ATCAGCTTTG ACAAGCACTG CGAGTGGCAG 
TCCAAGGAGG TCCGAACCAG ACCCAAGAGC GGATCCCTGC TGCTGTACTC GCGGAAGAAG 
GTGCGCTATC GCCGCGACGG CTATTGCTGG AAGAAGCGCA AGGATGGCAA GACGACGCGC 
GAGGATCACA TGAAACTGAA AGTACAAGGC ACGGAGTGCA TCTACGGCTG TTATGTACAC 
TCGGCCATAT TGCCCACGTT CCATCGCAGA TGTTACTGGC TGTTGCAGAA TCCGGACATT 
GTTTTGGTGC ACTATCTGAA TGTGCCGTAT CCGGATGATA ATAAAATGGC AGTGATTGCC 
CCCAGCATCA CCCTGTGGGG CGACAAGAAA GAGTGGACCA AGGAGGAGCT GGTCAGCCAG 
CTGAAGCCGA TGCTCTCCAC AGTCAACTCC GATGATGACT CCGACTCGGG CAACGACATT 
GAGATATCGA CGGCCGAGAC CGTGGAGTCG ATTGTGTGCC AGCTGATGGA GAAGCAGCGC 
TTGTCCCGCC AGGCGGCCCT CGTCAAGCAG CTCGACTGCG GCTGCGGGGA CTCGGGCTGT 
GCCGATGGCA AGACCTGCAC GCATCCCGTG ATGCGGCGCA CGGGCGGCAT CATCAAGTCC 
GCGGTGGCCA CATCGGCCTC AGAGAAGCGA CTCAACGAGG GGGCCTTCAA CGGCGGCACG 
CCCAATGTGG TGGTGGCCAG CAAGCTATAC TCCCGCTGGG CGGAGCGGCC GCGCCAGCGC 
AACGATGGCA TGCACCTGGA CAACCCGCAC GCACAGTTCC ACAATGCCAC GCAACAGCTG 
CCCCAGGAAT CGGCCATCAA GTTTCAGATC ATTCCTCCTC AACAGCAGCA GCAGCAGCAA 
CAACAGACGG GACATGGCGA TGGCAGCTAT CAGCAGCAAT ATCGTGGTGC CAATCAGATG 
CTGGCCGCAA GAAGCAATCT GGTGATGCAG CAGCAACAGC AACAGCAGCA GCAATATCAC 
CAGCAACAGC AGCAACACTT GAGTCTGCAA CGATTCGTGG CCAGTCAGAG CCAGAATTCG 
GCTCATTTGA ATCATCATCA TCATCGCCTG CAAATTGCCA ATACAACAAT CACGGCCACA 
GCCAGAGATC CTTCCACAAC AGTAGCAGCA GCAGCAACAG CAGCATCGGC ATCAGCAGCA 
GGAGGAGGCT CCGTCTTAAA TGGAATGGAT ACGGAACTAA TTGAAATCCA AAGCCACATG 
ACTGCAAACA AAATCACTAG CTCCGTCAGC AGCAATGGAA ATGGCAGCGG CAGCAATCCC 
AGTGCCAGTG CCAAGCAATT AGCAGCTCAA ACGAACAACG AATTAAATGT CGTAAATAAT 
AACGACCCCA GCAACAATAA CTTTATGTAC AATCAACAAC AGCAGCAACT GAATACTTCC 
AGCAACAGCA ACAACACACA GCAGCAGCAG CAACATTATT ATAAATTGCA ACAGACATCA 
GCAACCCCAA ACAGTGGCCA ACTCCAACTC CAAAATCAAA CCCAAACACA AACACAAACC 
CCAACCCTTG TCCAACCCCC TGTTGAGGCC ATGTGTATGT CACCTGAGCA TCGCAGCGGC 
AGCAGCAGCA GCAGCAACAA CAATACGGCC TCAACGTCTT CCGCTGTCAA CAGTCATAAC 
ACCTCCCCCT CCACAGCCAT AGCCACAACC ACGTCCATGG CCGCATCTAT ATCTATATCC 
ACATCTACGT CCGGCACATC GTCCACCTCG AGTTCCTTAC AATCGATTAT CGATGGCAAT 
CAGCAGCAAC AAATTACAAC GACATCGACG GCAGCAACTT GTGATAATTT AATGAGCGCC 
AATGCGCTCA CTGAGCTGAA TGGAGGGAGC AGCAGCAGCA GCAACAATCA GGCCACAGAT 
GGCCACAACA AGAGTCAATC CCAATCGCAG TCTCAGCCGC AAACTCTGAG CCAAAATGGT 
TGTGCAACAT TCAGTGCTTC CAGTGATAAT AGCAGCCAAA TTAGCGCCAG CGACGAGAGT 
AGCAGCTTTG GGGCCAACAA TCAGAACAAC AGCATACACA GCATCCACAG CAGCCACAGC 
AGCAGCAGTA ACAGCAGCGA GGACGAGCCC CAGGCGGGCA ATGCTGCCGA GACCCTGAGC 
TTCTTCAACG AAACCCTGGA TCTATCCCAT GAGGATATTC AACGTACACT GATAGCCAAT 
ATGCAGCCGT ACAATACGGC TGCAGGCGAA GCAGCAGCGA CAACGATAGA ACCTTTGGCA 
ACAAACGGCA GCCACAGTAG CAGCAACAGC AATGGCAATC CTCAAGAAGT GGAGAAGAAA 
CCTTCAGTCG GCCAGGAAGT CGAAGCCGAA CCTGAGGAGG ATGACACAGA CGATGTGTTT 
GCCAACCTGG ATGCCTTCGA TATGCTCGTG GAGTTCCCCG AGCTGGATCT GGATGATAAG 
CAGGCCCTGA ACAACACGGC CTTGGAGCAG GGCTCCTATC TGGGACAGGC GGCCGCCCAA 
ACGCAGCAGC CCCGCAAGAT CCACAACATC TGCGACTTCA GTCCGGAGTG GTCGTACACC 
GAGGGCGGAG TCAAGGTACT TGTGGCCGGA CCCTGGACGA GCAGCAATGG TGCTGGTGCC 
TACACGGTGC TCTTCGATGC CCAGCCCGTG CCCACGCAGA TGGTGCAGGA GGGAGTGCTC 
CGTTGCTACT GTCCTGCCCA CGAGGCGGGC TTTGTGACGC TCCAGGTGGC CTGCGGCGGC 
TTTCTCGTGT CCAATTCGGT CATGTTCGAG TACAAGCTGT CGCTGCTGGC CGATGCCCCG 
TTCGATGCGA GCAGCTCCAA CGATTGCCTG TACAAATTCA CGCTGCTCAA TCGCCTCTCG 
ACCATCGACG ACAAGTTGCA GCTGAAGACG GAGCAGGAGC CGACGACGGA CCACACGGCT 
CTCTATCTGG AGCCGAACTT TGAGGAGAAG CTGGTCGCGT ACTGCCACAG GCTGACGAAG 
CATGCCTGGA GCATGCCCAG CACAGTGGCC AGCTGGAGCG TGGGCCTGCG GGGCATGACG 
CTGCTGCATC TGGCCGCCGC CTTGGGCTAT GCCAAGCTGG TAGGCGCCAT GCTCAATTGG 
CGGGCGGAGA ATCCACATAT CATACTCGAA ACGGAGCTGG ACGCCCTCAG TCAGGATGTG 
TACGGTTTCA CGCCGCTCGC CTGGTCCTGT GTGCGCGGCC ATGTGGAGTG TTCCCTGCTG 
CTCTACAAGT GGAATCACAA TGCGCTGAAG ATCAAGACGC AGAGCCAGCA AACGGCCCTG 
GATTTGGCCA ACCTGAAGGG GCACAAACAT CTCCTGTCCC AGATATTTCG ACTGGAGAAG 
GAGCGCTGCC GCAAGCCGCA CTTGCGAGGC GGCCTGGCCA ATCTTTCAAT GAACCTAGGC 
GTTGAAACGG AAGCGGAGGA GTCGCCGCCG CAGACCTACA ACAGCTTTGA CCTGGAGATA 
CTCCAAAGAA AGCACGATGG CGTCTTCCTC AGACCTGGGG CAGTGCATAG CAATCAAAGT 
CCTCCCAACA ATGGCAGCCG GTACTCCAAG CGCTCTTCCA TAGACAGCGG CATCAACATG 
GACATACGCA GCAAGTCAGG CAAGTCCCTG GCCAGGCTGC ACAGCAACTT TGAGGGGCAC 
GACAGCTATG CATTGGGAGT GGACTCGCCG CTGGATACGC TGACTGGGAC CAATTGCCTG 
CTCTCACCGC TACGTAAAAT GGATTTCGCC CTGTGGGACT CGGATGCCAA AGTGCTGACT 
CTGGCCGAAC ACATTATAGC AGCAATGCCA GAGCGCATCA AAAATGAAGC CGATGAAATG 
ATGGTGCTGG GCAGCCCCTT GACTGAGCCA CTGACCTCGG AGTCCTCGGC CCTGACAGAC 
AGCTTTATGG ATCCCCTGCT GGACTCGCTG CCCAATACGC ATTTCGACAG CGACTTCAAC 
TTCGACTTCC ACGACCACAG CTATCGGTAC CACGATGTGA GCACTCCCTG CTCCAGCCTG 
AGTCCCGCCA GCTCGGGGCC GCTGCAGTCG CCGGCCAGCT ACTCCATACT GGGCACAGAT 
CCGTCTGTCA GCTCGCCCAG TCCGCCGCCG TCGACCAAGC AGCTGACCGA GTTCCTGCAC 
GCATCCAGCC TGTCGCAGTA TCCGTTCGAG GCGGACTTCT CCAAGCTGAC GCTGACCGAC 
ACGGAGCAGC GGGAGCTGTA CGAGGCGGCC AAGTGCATAC AGAAGGCCTA CCGCTCGTAC 
AAGGGCCGGC AGAAGCTCGA GGAGCAGAAC AAAGAGCGCA CTGCCGCCAT TGTCATCCAG 
AACTATTACA GGCGGTACAA GCAGTACGCC TATTACAGGC AGATGACCAA CGCCGCCCTG 
GTCATCCAGC ATGGCTATCG TTCCTATCGA AAGAACAAAC GCTTCAAGAA GAGCGGCCTG 
GGCCAGGGCA TGGCAGGGGG CAGCGATCAC GGCAGCGTCA GCAGCAACTC TCAGTGCCTG 
TCCAGCTTCT ACGACCACTA CAAGCAGGAT CAGCAGCAGC TGCAGCAGCA GCATGAGATG 
GGCTCGCAGC CGAGCACGCC CAAGGAAACC AGTCCCTCGG GTCCCTTGAA ACGAACCTAC 
TCGCAGTCCA CCCAGAATCA AGCTGCCAGG AAGATCCAAC AGTTCATGAG ACAATCACGC 
ATCAAACTAC AGAGAGAGCG AGCCGAAAAA GAGAAGCTGG TGCACCAACG CAGGGCGGAA 
TACCTTCAAA ACTTGCAGTA TCAAGGGCAG CAGGAAATCT TGGCTTGCCT AGAAAATAAC 
AGTTCTGCCC AGAGCAGTGG CAGCAACACC TATACGAACA GCATCAACAA TCTACAATCC 
AATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA30460 ( XM_015184949.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015040435.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant D (Dpse\GA30460), mRNA.

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CloneID	ODe23356
Clone ID Related Accession (Same CDS sequence)	XM_015184949.1
Accession Version	XM_015184949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform D
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGGTT ATCGCTCGTT GCCGCTGGCC CCCGTTTCGG CCGGCAGTAT GGCAGCGGCG 
GCGGCCGCCG TTCCGGCCCA AAATGTGGGT CATGCCGGAC CCACGATGGC CTATGCCCAC 
CACCAGTCGA TGACGGCCAT GTCATCGAAT AACGGTATTA TCTATCAGTC GGCGGTGCAC 
GCCTCCATAC ACAGCCTCCC GAGCCAGCAT CGCTCATCCA CCACGCATCA TCACATTCTA 
GCCACGGCCA CCGCCACAGC AACGGCCGCA GCAGCAGCAG CGGCAGCCAC AGCCGCCGCA 
GCAATGGTGT CCCTGCCGGC GACAGCAGCA GCAGCAGCAC AGCAACACGG AACCTATGGA 
CACCAGCAGC AGCCACACCA CCCACAACAG CAGCAGCAGC AGCAACAACA GCAGCAGCAG 
CAGCATCTTC CTCAGCAATC CCATCAGCAG CAGCAGCAGC AGCAGCAGCA TCAGCAACAA 
CTGATGTCCC ATCAGCAGCA TTCCGCGTTG CAGCAGCAGC AGCAACAACA TCACCATCTA 
CAGCAATCCC ATCAGCAACA GCAACAGCTC CATCAGCAGC AACAGCAACA GCAGCAGCAG 
CAGCATCTCT CTGCCGTGCA CCACCACCAT CATCACCACC ACCAGCAGCT GTCGCTGCAC 
CAGAACCACT TGTACTTCAG CGGTGGGAGT CTGGGGCACC ATCACCACCA TCGACAGATC 
TTCAGCCACC TGCAGAGCGC CCTAATGCCG CTCAGTGTCA ATGGCGAACC TATCAAACTA 
CCCGACAATT TGGAGAGCCT GCCAAGAGCT GACAGTTTTC CATCACAGCG TCATCGTTGG 
AATACAAATG AGGAAATTGC TGCGATTCTT ATCAGCTTTG ACAAGCACTG CGAGTGGCAG 
TCCAAGGAGG TCCGAACCAG ACCCAAGAGC GGATCCCTGC TGCTGTACTC GCGGAAGAAG 
GTGCGCTATC GCCGCGACGG CTATTGCTGG AAGAAGCGCA AGGATGGCAA GACGACGCGC 
GAGGATCACA TGAAACTGAA AGTACAAGGC ACGGAGTGCA TCTACGGCTG TTATGTACAC 
TCGGCCATAT TGCCCACGTT CCATCGCAGA TGTTACTGGC TGTTGCAGAA TCCGGACATT 
GTTTTGGTGC ACTATCTGAA TGTGCCGTAT CCGGATGATA ATAAAATGGC AGTGATTGCC 
CCCAGCATCA CCCTGTGGGG CGACAAGAAA GAGTGGACCA AGGAGGAGCT GGTCAGCCAG 
CTGAAGCCGA TGCTCTCCAC AGTCAACTCC GATGATGACT CCGACTCGGG CAACGACATT 
GAGATATCGA CGGCCGAGAC CGTGGAGTCG ATTGTGTGCC AGCTGATGGA GAAGCAGCGC 
TTGTCCCGCC AGGCGGCCCT CGTCAAGCAG CTCGACTGCG GCTGCGGGGA CTCGGGCTGT 
GCCGATGGCA AGACCTGCAC GCATCCCGTG ATGCGGCGCA CGGGCGGCAT CATCAAGTCC 
GCGGTGGCCA CATCGGCCTC AGAGAAGCGA CTCAACGAGG GGGCCTTCAA CGGCGGCACG 
CCCAATGTGG TGGTGGCCAG CAAGCTATAC TCCCGCTGGG CGGAGCGGCC GCGCCAGCGC 
AACGATGGCA TGCACCTGGA CAACCCGCAC GCACAGTTCC ACAATGCCAC GCAACAGCTG 
CCCCAGGAAT CGGCCATCAA GTTTCAGATC ATTCCTCCTC AACAGCAGCA GCAGCAGCAA 
CAACAGACGG GACATGGCGA TGGCAGCTAT CAGCAGCAAT ATCGTGGTGC CAATCAGATG 
CTGGCCGCAA GAAGCAATCT GGTGATGCAG CAGCAACAGC AACAGCAGCA GCAATATCAC 
CAGCAACAGC AGCAACACTT GAGTCTGCAA CGATTCGTGG CCAGTCAGAG CCAGAATTCG 
GCTCATTTGA ATCATCATCA TCATCGCCTG CAAATTGCCA ATACAACAAT CACGGCCACA 
GCCAGAGATC CTTCCACAAC AGTAGCAGCA GCAGCAACAG CAGCATCGGC ATCAGCAGCA 
GGAGGAGGCT CCGTCTTAAA TGGAATGGAT ACGGAACTAA TTGAAATCCA AAGCCACATG 
ACTGCAAACA AAATCACTAG CTCCGTCAGC AGCAATGGAA ATGGCAGCGG CAGCAATCCC 
AGTGCCAGTG CCAAGCAATT AGCAGCTCAA ACGAACAACG AATTAAATGT CGTAAATAAT 
AACGACCCCA GCAACAATAA CTTTATGTAC AATCAACAAC AGCAGCAACT GAATACTTCC 
AGCAACAGCA ACAACACACA GCAGCAGCAG CAACATTATT ATAAATTGCA ACAGACATCA 
GCAACCCCAA ACAGTGGCCA ACTCCAACTC CAAAATCAAA CCCAAACACA AACACAAACC 
CCAACCCTTG TCCAACCCCC TGTTGAGGCC ATGTGTATGT CACCTGAGCA TCGCAGCGGC 
AGCAGCAGCA GCAGCAACAA CAATACGGCC TCAACGTCTT CCGCTGTCAA CAGTCATAAC 
ACCTCCCCCT CCACAGCCAT AGCCACAACC ACGTCCATGG CCGCATCTAT ATCTATATCC 
ACATCTACGT CCGGCACATC GTCCACCTCG AGTTCCTTAC AATCGATTAT CGATGGCAAT 
CAGCAGCAAC AAATTACAAC GACATCGACG GCAGCAACTT GTGATAATTT AATGAGCGCC 
AATGCGCTCA CTGAGCTGAA TGGAGGGAGC AGCAGCAGCA GCAACAATCA GGCCACAGAT 
GGCCACAACA AGAGTCAATC CCAATCGCAG TCTCAGCCGC AAACTCTGAG CCAAAATGGT 
TGTGCAACAT TCAGTGCTTC CAGTGATAAT AGCAGCCAAA TTAGCGCCAG CGACGAGAGT 
AGCAGCTTTG GGGCCAACAA TCAGAACAAC AGCATACACA GCATCCACAG CAGCCACAGC 
AGCAGCAGTA ACAGCAGCGA GGACGAGCCC CAGGCGGGCA ATGCTGCCGA GACCCTGAGC 
TTCTTCAACG AAACCCTGGA TCTATCCCAT GAGGATATTC AACGTACACT GATAGCCAAT 
ATGCAGCCGT ACAATACGGC TGCAGGCGAA GCAGCAGCGA CAACGATAGA ACCTTTGGCA 
ACAAACGGCA GCCACAGTAG CAGCAACAGC AATGGCAATC CTCAAGAAGT GGAGAAGAAA 
CCTTCAGTCG GCCAGGAAGT CGAAGCCGAA CCTGAGGAGG ATGACACAGA CGATGTGTTT 
GCCAACCTGG ATGCCTTCGA TATGCTCGTG GAGTTCCCCG AGCTGGATCT GGATGATAAG 
CAGGCCCTGA ACAACACGGC CTTGGAGCAG GGCTCCTATC TGGGACAGGC GGCCGCCCAA 
ACGCAGCAGC CCCGCAAGAT CCACAACATC TGCGACTTCA GTCCGGAGTG GTCGTACACC 
GAGGGCGGAG TCAAGGTACT TGTGGCCGGA CCCTGGACGA GCAGCAATGG TGCTGGTGCC 
TACACGGTGC TCTTCGATGC CCAGCCCGTG CCCACGCAGA TGGTGCAGGA GGGAGTGCTC 
CGTTGCTACT GTCCTGCCCA CGAGGCGGGC TTTGTGACGC TCCAGGTGGC CTGCGGCGGC 
TTTCTCGTGT CCAATTCGGT CATGTTCGAG TACAAGCTGT CGCTGCTGGC CGATGCCCCG 
TTCGATGCGA GCAGCTCCAA CGATTGCCTG TACAAATTCA CGCTGCTCAA TCGCCTCTCG 
ACCATCGACG ACAAGTTGCA GCTGAAGACG GAGCAGGAGC CGACGACGGA CCACACGGCT 
CTCTATCTGG AGCCGAACTT TGAGGAGAAG CTGGTCGCGT ACTGCCACAG GCTGACGAAG 
CATGCCTGGA GCATGCCCAG CACAGTGGCC AGCTGGAGCG TGGGCCTGCG GGGCATGACG 
CTGCTGCATC TGGCCGCCGC CTTGGGCTAT GCCAAGCTGG TAGGCGCCAT GCTCAATTGG 
CGGGCGGAGA ATCCACATAT CATACTCGAA ACGGAGCTGG ACGCCCTCAG TCAGGATGTG 
TACGGTTTCA CGCCGCTCGC CTGGTCCTGT GTGCGCGGCC ATGTGGAGTG TTCCCTGCTG 
CTCTACAAGT GGAATCACAA TGCGCTGAAG ATCAAGACGC AGAGCCAGCA AACGGCCCTG 
GATTTGGCCA ACCTGAAGGG GCACAAACAT CTCCTGTCCC AGATATTTCG ACTGGAGAAG 
GAGCGCTGCC GCAAGCCGCA CTTGCGAGGC GGCCTGGCCA ATCTTTCAAT GAACCTAGGC 
GTTGAAACGG AAGCGGAGGA GTCGCCGCCG CAGACCTACA ACAGCTTTGA CCTGGAGATA 
CTCCAAAGAA AGCACGATGG CGTCTTCCTC AGACCTGGGG CAGTGCATAG CAATCAAAGT 
CCTCCCAACA ATGGCAGCCG GTACTCCAAG CGCTCTTCCA TAGACAGCGG CATCAACATG 
GACATACGCA GCAAGTCAGG CAAGTCCCTG GCCAGGCTGC ACAGCAACTT TGAGGGGCAC 
GACAGCTATG CATTGGGAGT GGACTCGCCG CTGGATACGC TGACTGGGAC CAATTGCCTG 
CTCTCACCGC TACGTAAAAT GGATTTCGCC CTGTGCGAGG TATCCACGGG CGAGTCGAGC 
CCCATACACG ACAAAGACAT CGATGGCGAT GACACCTCGA CGAGCGCCAC CGATGTGACC 
ATAGGCAATG ACCTGGCCCT GGCCCTGGCC GATGCAGTTG TAGGGGACTC GGATGCCAAA 
GTGCTGACTC TGGCCGAACA CATTATAGCA GCAATGCCAG AGCGCATCAA AAATGAAGCC 
GATGAAATGA TGGTGCTGGG CAGCCCCTTG ACTGAGCCAC TGACCTCGGA GTCCTCGGCC 
CTGACAGACA GCTTTATGGA TCCCCTGCTG GACTCGCTGC CCAATACGCA TTTCGACAGC 
GACTTCAACT TCGACTTCCA CGACCACAGC TATCGGTACC ACGATGTGAG CACTCCCTGC 
TCCAGCCTGA GTCCCGCCAG CTCGGGGCCG CTGCAGTCGC CGGCCAGCTA CTCCATACTG 
GGCACAGATC CGTCTGTCAG CTCGCCCAGT CCGCCGCCGT CGACCAAGCA GCTGACCGAG 
TTCCTGCACG CATCCAGCCT GTCGCAGTAT CCGTTCGAGG CGGACTTCTC CAAGCTGACG 
CTGACCGACA CGGAGCAGCG GGAGCTGTAC GAGGCGGCCA AGTGCATACA GAAGGCCTAC 
CGCTCGTACA AGGGCCGGCA GAAGCTCGAG GAGCAGAACA AAGAGCGCAC TGCCGCCATT 
GTCATCCAGA ACTATTACAG GCGGTACAAG CAGTACGCCT ATTACAGGCA GATGACCAAC 
GCCGCCCTGG TCATCCAGCA TGGCTATCGT TCCTATCGAA AGAACAAACG CTTCAAGAAG 
AGCGGCCTGG GCCAGGGCAT GGCAGGGGGC AGCGATCACG GCAGCGTCAG CAGCAACTCT 
CAGTGCCTGT CCAGCTTCTA CGACCACTAC AAGCAGGATC AGCAGCAGCT GCAGCAGCAG 
CATGAGATGG GCTCGCAGCC GAGCACGCCC AAGGAAACCA GTCCCTCGGG TCCCTTGAAA 
CGAACCTACT CGCAGTCCAC CCAGAATCAA GCTGCCAGGA AGATCCAACA GTTCATGAGA 
CAATCACGCA TCAAACTACA GAGAGAGCGA GCCGAAAAAG AGAAGCTGGT GCACCAACGC 
AGGGCGGAAT ACCTTCAAAA CTTGCAGTAT CAAGGGCAGC AGGAAATCTT GGCTTGCCTA 
GAAAATAACA GTTCTGCCCA GAGCAGTGGC AGCAACACCT ATACGAACAG CATCAACAAT 
CTACAATCCA ATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015040435.1
CDS	220..6057
Translation	MAGYRSLPLAPVSAGSMAAAAAAVPAQNVGHAGPTMAYAHHQSMTAMSSNNGIIYQSAVHASIHSLPSQHRSSTTHHHILATATATATAAAAAAAATAAAAMVSLPATAAAAAQQHGTYGHQQQPHHPQQQQQQQQQQQQQHLPQQSHQQQQQQQQHQQQLMSHQQHSALQQQQQQHHHLQQSHQQQQQLHQQQQQQQQQQHLSAVHHHHHHHHQQLSLHQNHLYFSGGSLGHHHHHRQIFSHLQSALMPLSVNGEPIKLPDNLESLPRADSFPSQRHRWNTNEEIAAILISFDKHCEWQSKEVRTRPKSGSLLLYSRKKVRYRRDGYCWKKRKDGKTTREDHMKLKVQGTECIYGCYVHSAILPTFHRRCYWLLQNPDIVLVHYLNVPYPDDNKMAVIAPSITLWGDKKEWTKEELVSQLKPMLSTVNSDDDSDSGNDIEISTAETVESIVCQLMEKQRLSRQAALVKQLDCGCGDSGCADGKTCTHPVMRRTGGIIKSAVATSASEKRLNEGAFNGGTPNVVVASKLYSRWAERPRQRNDGMHLDNPHAQFHNATQQLPQESAIKFQIIPPQQQQQQQQQTGHGDGSYQQQYRGANQMLAARSNLVMQQQQQQQQQYHQQQQQHLSLQRFVASQSQNSAHLNHHHHRLQIANTTITATARDPSTTVAAAATAASASAAGGGSVLNGMDTELIEIQSHMTANKITSSVSSNGNGSGSNPSASAKQLAAQTNNELNVVNNNDPSNNNFMYNQQQQQLNTSSNSNNTQQQQQHYYKLQQTSATPNSGQLQLQNQTQTQTQTPTLVQPPVEAMCMSPEHRSGSSSSSNNNTASTSSAVNSHNTSPSTAIATTTSMAASISISTSTSGTSSTSSSLQSIIDGNQQQQITTTSTAATCDNLMSANALTELNGGSSSSSNNQATDGHNKSQSQSQSQPQTLSQNGCATFSASSDNSSQISASDESSSFGANNQNNSIHSIHSSHSSSSNSSEDEPQAGNAAETLSFFNETLDLSHEDIQRTLIANMQPYNTAAGEAAATTIEPLATNGSHSSSNSNGNPQEVEKKPSVGQEVEAEPEEDDTDDVFANLDAFDMLVEFPELDLDDKQALNNTALEQGSYLGQAAAQTQQPRKIHNICDFSPEWSYTEGGVKVLVAGPWTSSNGAGAYTVLFDAQPVPTQMVQEGVLRCYCPAHEAGFVTLQVACGGFLVSNSVMFEYKLSLLADAPFDASSSNDCLYKFTLLNRLSTIDDKLQLKTEQEPTTDHTALYLEPNFEEKLVAYCHRLTKHAWSMPSTVASWSVGLRGMTLLHLAAALGYAKLVGAMLNWRAENPHIILETELDALSQDVYGFTPLAWSCVRGHVECSLLLYKWNHNALKIKTQSQQTALDLANLKGHKHLLSQIFRLEKERCRKPHLRGGLANLSMNLGVETEAEESPPQTYNSFDLEILQRKHDGVFLRPGAVHSNQSPPNNGSRYSKRSSIDSGINMDIRSKSGKSLARLHSNFEGHDSYALGVDSPLDTLTGTNCLLSPLRKMDFALCEVSTGESSPIHDKDIDGDDTSTSATDVTIGNDLALALADAVVGDSDAKVLTLAEHIIAAMPERIKNEADEMMVLGSPLTEPLTSESSALTDSFMDPLLDSLPNTHFDSDFNFDFHDHSYRYHDVSTPCSSLSPASSGPLQSPASYSILGTDPSVSSPSPPPSTKQLTEFLHASSLSQYPFEADFSKLTLTDTEQRELYEAAKCIQKAYRSYKGRQKLEEQNKERTAAIVIQNYYRRYKQYAYYRQMTNAALVIQHGYRSYRKNKRFKKSGLGQGMAGGSDHGSVSSNSQCLSSFYDHYKQDQQQLQQQHEMGSQPSTPKETSPSGPLKRTYSQSTQNQAARKIQQFMRQSRIKLQRERAEKEKLVHQRRAEYLQNLQYQGQQEILACLENNSSAQSSGSNTYTNSINNLQSNQ

Target ORF information:

RefSeq Version	XM_015184949.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant D (Dpse\GA30460), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015184949.1

ORF Insert Sequence:

ATGGCCGGTT ATCGCTCGTT GCCGCTGGCC CCCGTTTCGG CCGGCAGTAT GGCAGCGGCG 
GCGGCCGCCG TTCCGGCCCA AAATGTGGGT CATGCCGGAC CCACGATGGC CTATGCCCAC 
CACCAGTCGA TGACGGCCAT GTCATCGAAT AACGGTATTA TCTATCAGTC GGCGGTGCAC 
GCCTCCATAC ACAGCCTCCC GAGCCAGCAT CGCTCATCCA CCACGCATCA TCACATTCTA 
GCCACGGCCA CCGCCACAGC AACGGCCGCA GCAGCAGCAG CGGCAGCCAC AGCCGCCGCA 
GCAATGGTGT CCCTGCCGGC GACAGCAGCA GCAGCAGCAC AGCAACACGG AACCTATGGA 
CACCAGCAGC AGCCACACCA CCCACAACAG CAGCAGCAGC AGCAACAACA GCAGCAGCAG 
CAGCATCTTC CTCAGCAATC CCATCAGCAG CAGCAGCAGC AGCAGCAGCA TCAGCAACAA 
CTGATGTCCC ATCAGCAGCA TTCCGCGTTG CAGCAGCAGC AGCAACAACA TCACCATCTA 
CAGCAATCCC ATCAGCAACA GCAACAGCTC CATCAGCAGC AACAGCAACA GCAGCAGCAG 
CAGCATCTCT CTGCCGTGCA CCACCACCAT CATCACCACC ACCAGCAGCT GTCGCTGCAC 
CAGAACCACT TGTACTTCAG CGGTGGGAGT CTGGGGCACC ATCACCACCA TCGACAGATC 
TTCAGCCACC TGCAGAGCGC CCTAATGCCG CTCAGTGTCA ATGGCGAACC TATCAAACTA 
CCCGACAATT TGGAGAGCCT GCCAAGAGCT GACAGTTTTC CATCACAGCG TCATCGTTGG 
AATACAAATG AGGAAATTGC TGCGATTCTT ATCAGCTTTG ACAAGCACTG CGAGTGGCAG 
TCCAAGGAGG TCCGAACCAG ACCCAAGAGC GGATCCCTGC TGCTGTACTC GCGGAAGAAG 
GTGCGCTATC GCCGCGACGG CTATTGCTGG AAGAAGCGCA AGGATGGCAA GACGACGCGC 
GAGGATCACA TGAAACTGAA AGTACAAGGC ACGGAGTGCA TCTACGGCTG TTATGTACAC 
TCGGCCATAT TGCCCACGTT CCATCGCAGA TGTTACTGGC TGTTGCAGAA TCCGGACATT 
GTTTTGGTGC ACTATCTGAA TGTGCCGTAT CCGGATGATA ATAAAATGGC AGTGATTGCC 
CCCAGCATCA CCCTGTGGGG CGACAAGAAA GAGTGGACCA AGGAGGAGCT GGTCAGCCAG 
CTGAAGCCGA TGCTCTCCAC AGTCAACTCC GATGATGACT CCGACTCGGG CAACGACATT 
GAGATATCGA CGGCCGAGAC CGTGGAGTCG ATTGTGTGCC AGCTGATGGA GAAGCAGCGC 
TTGTCCCGCC AGGCGGCCCT CGTCAAGCAG CTCGACTGCG GCTGCGGGGA CTCGGGCTGT 
GCCGATGGCA AGACCTGCAC GCATCCCGTG ATGCGGCGCA CGGGCGGCAT CATCAAGTCC 
GCGGTGGCCA CATCGGCCTC AGAGAAGCGA CTCAACGAGG GGGCCTTCAA CGGCGGCACG 
CCCAATGTGG TGGTGGCCAG CAAGCTATAC TCCCGCTGGG CGGAGCGGCC GCGCCAGCGC 
AACGATGGCA TGCACCTGGA CAACCCGCAC GCACAGTTCC ACAATGCCAC GCAACAGCTG 
CCCCAGGAAT CGGCCATCAA GTTTCAGATC ATTCCTCCTC AACAGCAGCA GCAGCAGCAA 
CAACAGACGG GACATGGCGA TGGCAGCTAT CAGCAGCAAT ATCGTGGTGC CAATCAGATG 
CTGGCCGCAA GAAGCAATCT GGTGATGCAG CAGCAACAGC AACAGCAGCA GCAATATCAC 
CAGCAACAGC AGCAACACTT GAGTCTGCAA CGATTCGTGG CCAGTCAGAG CCAGAATTCG 
GCTCATTTGA ATCATCATCA TCATCGCCTG CAAATTGCCA ATACAACAAT CACGGCCACA 
GCCAGAGATC CTTCCACAAC AGTAGCAGCA GCAGCAACAG CAGCATCGGC ATCAGCAGCA 
GGAGGAGGCT CCGTCTTAAA TGGAATGGAT ACGGAACTAA TTGAAATCCA AAGCCACATG 
ACTGCAAACA AAATCACTAG CTCCGTCAGC AGCAATGGAA ATGGCAGCGG CAGCAATCCC 
AGTGCCAGTG CCAAGCAATT AGCAGCTCAA ACGAACAACG AATTAAATGT CGTAAATAAT 
AACGACCCCA GCAACAATAA CTTTATGTAC AATCAACAAC AGCAGCAACT GAATACTTCC 
AGCAACAGCA ACAACACACA GCAGCAGCAG CAACATTATT ATAAATTGCA ACAGACATCA 
GCAACCCCAA ACAGTGGCCA ACTCCAACTC CAAAATCAAA CCCAAACACA AACACAAACC 
CCAACCCTTG TCCAACCCCC TGTTGAGGCC ATGTGTATGT CACCTGAGCA TCGCAGCGGC 
AGCAGCAGCA GCAGCAACAA CAATACGGCC TCAACGTCTT CCGCTGTCAA CAGTCATAAC 
ACCTCCCCCT CCACAGCCAT AGCCACAACC ACGTCCATGG CCGCATCTAT ATCTATATCC 
ACATCTACGT CCGGCACATC GTCCACCTCG AGTTCCTTAC AATCGATTAT CGATGGCAAT 
CAGCAGCAAC AAATTACAAC GACATCGACG GCAGCAACTT GTGATAATTT AATGAGCGCC 
AATGCGCTCA CTGAGCTGAA TGGAGGGAGC AGCAGCAGCA GCAACAATCA GGCCACAGAT 
GGCCACAACA AGAGTCAATC CCAATCGCAG TCTCAGCCGC AAACTCTGAG CCAAAATGGT 
TGTGCAACAT TCAGTGCTTC CAGTGATAAT AGCAGCCAAA TTAGCGCCAG CGACGAGAGT 
AGCAGCTTTG GGGCCAACAA TCAGAACAAC AGCATACACA GCATCCACAG CAGCCACAGC 
AGCAGCAGTA ACAGCAGCGA GGACGAGCCC CAGGCGGGCA ATGCTGCCGA GACCCTGAGC 
TTCTTCAACG AAACCCTGGA TCTATCCCAT GAGGATATTC AACGTACACT GATAGCCAAT 
ATGCAGCCGT ACAATACGGC TGCAGGCGAA GCAGCAGCGA CAACGATAGA ACCTTTGGCA 
ACAAACGGCA GCCACAGTAG CAGCAACAGC AATGGCAATC CTCAAGAAGT GGAGAAGAAA 
CCTTCAGTCG GCCAGGAAGT CGAAGCCGAA CCTGAGGAGG ATGACACAGA CGATGTGTTT 
GCCAACCTGG ATGCCTTCGA TATGCTCGTG GAGTTCCCCG AGCTGGATCT GGATGATAAG 
CAGGCCCTGA ACAACACGGC CTTGGAGCAG GGCTCCTATC TGGGACAGGC GGCCGCCCAA 
ACGCAGCAGC CCCGCAAGAT CCACAACATC TGCGACTTCA GTCCGGAGTG GTCGTACACC 
GAGGGCGGAG TCAAGGTACT TGTGGCCGGA CCCTGGACGA GCAGCAATGG TGCTGGTGCC 
TACACGGTGC TCTTCGATGC CCAGCCCGTG CCCACGCAGA TGGTGCAGGA GGGAGTGCTC 
CGTTGCTACT GTCCTGCCCA CGAGGCGGGC TTTGTGACGC TCCAGGTGGC CTGCGGCGGC 
TTTCTCGTGT CCAATTCGGT CATGTTCGAG TACAAGCTGT CGCTGCTGGC CGATGCCCCG 
TTCGATGCGA GCAGCTCCAA CGATTGCCTG TACAAATTCA CGCTGCTCAA TCGCCTCTCG 
ACCATCGACG ACAAGTTGCA GCTGAAGACG GAGCAGGAGC CGACGACGGA CCACACGGCT 
CTCTATCTGG AGCCGAACTT TGAGGAGAAG CTGGTCGCGT ACTGCCACAG GCTGACGAAG 
CATGCCTGGA GCATGCCCAG CACAGTGGCC AGCTGGAGCG TGGGCCTGCG GGGCATGACG 
CTGCTGCATC TGGCCGCCGC CTTGGGCTAT GCCAAGCTGG TAGGCGCCAT GCTCAATTGG 
CGGGCGGAGA ATCCACATAT CATACTCGAA ACGGAGCTGG ACGCCCTCAG TCAGGATGTG 
TACGGTTTCA CGCCGCTCGC CTGGTCCTGT GTGCGCGGCC ATGTGGAGTG TTCCCTGCTG 
CTCTACAAGT GGAATCACAA TGCGCTGAAG ATCAAGACGC AGAGCCAGCA AACGGCCCTG 
GATTTGGCCA ACCTGAAGGG GCACAAACAT CTCCTGTCCC AGATATTTCG ACTGGAGAAG 
GAGCGCTGCC GCAAGCCGCA CTTGCGAGGC GGCCTGGCCA ATCTTTCAAT GAACCTAGGC 
GTTGAAACGG AAGCGGAGGA GTCGCCGCCG CAGACCTACA ACAGCTTTGA CCTGGAGATA 
CTCCAAAGAA AGCACGATGG CGTCTTCCTC AGACCTGGGG CAGTGCATAG CAATCAAAGT 
CCTCCCAACA ATGGCAGCCG GTACTCCAAG CGCTCTTCCA TAGACAGCGG CATCAACATG 
GACATACGCA GCAAGTCAGG CAAGTCCCTG GCCAGGCTGC ACAGCAACTT TGAGGGGCAC 
GACAGCTATG CATTGGGAGT GGACTCGCCG CTGGATACGC TGACTGGGAC CAATTGCCTG 
CTCTCACCGC TACGTAAAAT GGATTTCGCC CTGTGCGAGG TATCCACGGG CGAGTCGAGC 
CCCATACACG ACAAAGACAT CGATGGCGAT GACACCTCGA CGAGCGCCAC CGATGTGACC 
ATAGGCAATG ACCTGGCCCT GGCCCTGGCC GATGCAGTTG TAGGGGACTC GGATGCCAAA 
GTGCTGACTC TGGCCGAACA CATTATAGCA GCAATGCCAG AGCGCATCAA AAATGAAGCC 
GATGAAATGA TGGTGCTGGG CAGCCCCTTG ACTGAGCCAC TGACCTCGGA GTCCTCGGCC 
CTGACAGACA GCTTTATGGA TCCCCTGCTG GACTCGCTGC CCAATACGCA TTTCGACAGC 
GACTTCAACT TCGACTTCCA CGACCACAGC TATCGGTACC ACGATGTGAG CACTCCCTGC 
TCCAGCCTGA GTCCCGCCAG CTCGGGGCCG CTGCAGTCGC CGGCCAGCTA CTCCATACTG 
GGCACAGATC CGTCTGTCAG CTCGCCCAGT CCGCCGCCGT CGACCAAGCA GCTGACCGAG 
TTCCTGCACG CATCCAGCCT GTCGCAGTAT CCGTTCGAGG CGGACTTCTC CAAGCTGACG 
CTGACCGACA CGGAGCAGCG GGAGCTGTAC GAGGCGGCCA AGTGCATACA GAAGGCCTAC 
CGCTCGTACA AGGGCCGGCA GAAGCTCGAG GAGCAGAACA AAGAGCGCAC TGCCGCCATT 
GTCATCCAGA ACTATTACAG GCGGTACAAG CAGTACGCCT ATTACAGGCA GATGACCAAC 
GCCGCCCTGG TCATCCAGCA TGGCTATCGT TCCTATCGAA AGAACAAACG CTTCAAGAAG 
AGCGGCCTGG GCCAGGGCAT GGCAGGGGGC AGCGATCACG GCAGCGTCAG CAGCAACTCT 
CAGTGCCTGT CCAGCTTCTA CGACCACTAC AAGCAGGATC AGCAGCAGCT GCAGCAGCAG 
CATGAGATGG GCTCGCAGCC GAGCACGCCC AAGGAAACCA GTCCCTCGGG TCCCTTGAAA 
CGAACCTACT CGCAGTCCAC CCAGAATCAA GCTGCCAGGA AGATCCAACA GTTCATGAGA 
CAATCACGCA TCAAACTACA GAGAGAGCGA GCCGAAAAAG AGAAGCTGGT GCACCAACGC 
AGGGCGGAAT ACCTTCAAAA CTTGCAGTAT CAAGGGCAGC AGGAAATCTT GGCTTGCCTA 
GAAAATAACA GTTCTGCCCA GAGCAGTGGC AGCAACACCT ATACGAACAG CATCAACAAT 
CTACAATCCA ATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA30460 ( XM_004444334.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_004444391.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA30460), mRNA.

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CloneID	ODe06604
Clone ID Related Accession (Same CDS sequence)	XM_004444334.2
Accession Version	XM_004444334.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_004444334.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAATG TGTCCGATGC CAGCTGCAGC GCCTCTTCCC TGAAACTCCA ACGGCAGCAA 
ACTCAAATCA AAGCCAAAAA TGTCCTAATC AGATCCCAGA GCAGCGGCAA GCTTAGCAGA 
AATGAGGCTG AATCCACAAC AACAGCAACA ACAACAGCAA CAGCGAAAAC CACAAAAGCC 
ACAACGAGGA CTCTCAAGAT CGGGGCCATG CAACAGAAGC AGCTACAGCC ACCAAATGGA 
GTCAACGCTA GCAACATCAT CCTGCTGCGG GGCACACGCA ACGAGAACGG CCAGATCATT 
ATCCAGAACA AGCAGGACAT CCTCACCCTG CTCAACGAGC AGCAGCAGCA CCAGGAGAAG 
GCAGCCCAGG CGGCCCAGGC GTCCACAGCC ATCACACTCA ATCAACTGCC AACGACCACC 
ACAGTGGCTC GCAAGACGAT TGTCCAGAGC TCGGGGAGCA GCACCACCAG CACCAGCTCC 
ACCATATCCA TCGTGGCCAG CAGCAGCAGC GGCAGCAGTA GCGGTGTTGG TGCCAAGGAT 
GCCGCTAGCA ACACGATCCT CCTGCAGACG CCCATCAATG CCAGCCAGCT GGAGAGCGTG 
CTGAAGGCCC AGGAGCGAAC GAAACAGGCG AACGGCACGG CACAGACCAA GCTCATAGAG 
AGGCCCTTCC TGCTGAAGCA CGCCACGCGG AGCCTCAGCT CGGAGAGTAA TTGTGACAGC 
AAATCGCCGT TCGTGCTGCA GACACTCAAG CGACTGGAGA AATCGCAATC CATTCTGGTC 
ATACGCAACT CCACATCAGG CAGCACTTCC TCAATGGCCG TTTCTCCGCC TGTGAATGTT 
TCGGCCTCCG TATCATCGAA TGTTTTAAGC GTGACACGCA CTTCAAAGGC GGCCAAGGGC 
ATGGCAGGTG CGCTGCACAA GCTCAAGGCG GCCGCAACGG CAGCAGCCGC AGCGGGAGCA 
GGAGCAGGGG CAGCACCATC CGCATCATCG ACAGGCACAA CCATACTGAG GCTAGGCAAA 
AGCGCCACCA CATTGGCCAT TAACGGAGCT GGGGGCAGCA GCAAACTGGA GGTCGCCGCC 
GCCGCCGCAA CAGCAACCAC AACGTCAACA GCAAATAAAT TGTCCAACAC GGTGACATCG 
GAGCAGCAGC AACAGCAACA ATCGACAACA ACGCAAACGA ATGTGCCACT TGGGAACGAT 
GGCGAACCTA TCAAACTACC CGACAATTTG GAGAGCCTGC CAAGAGCTGA CAGTTTTCCA 
TCACAGCGTC ATCGTTGGAA TACAAATGAG GAAATTGCTG CGATTCTTAT CAGCTTTGAC 
AAGCACTGCG AGTGGCAGTC CAAGGAGGTC CGAACCAGAC CCAAGAGCGG ATCCCTGCTG 
CTGTACTCGC GGAAGAAGGT GCGCTATCGC CGCGACGGCT ATTGCTGGAA GAAGCGCAAG 
GATGGCAAGA CGACGCGCGA GGATCACATG AAACTGAAAG TACAAGGCAC GGAGTGCATC 
TACGGCTGTT ATGTACACTC GGCCATATTG CCCACGTTCC ATCGCAGATG TTACTGGCTG 
TTGCAGAATC CGGACATTGT TTTGGTGCAC TATCTGAATG TGCCGTATCC GGATGATAAT 
AAAATGGCAG TGATTGCCCC CAGCATCACC CTGTGGGGCG ACAAGAAAGA GTGGACCAAG 
GAGGAGCTGG TCAGCCAGCT GAAGCCGATG CTCTCCACAG TCAACTCCGA TGATGACTCC 
GACTCGGGCA ACGACATTGA GATATCGACG GCCGAGACCG TGGAGTCGAT TGTGTGCCAG 
CTGATGGAGA AGCAGCGCTT GTCCCGCCAG GCGGCCCTCG TCAAGCAGCT CGACTGCGGC 
TGCGGGGACT CGGGCTGTGC CGATGGCAAG ACCTGCACGC ATCCCGTGAT GCGGCGCACG 
GGCGGCATCA TCAAGTCCGC GGTGGCCACA TCGGCCTCAG AGAAGCGACT CAACGAGGGG 
GCCTTCAACG GCGGCACGCC CAATGTGGTG GTGGCCAGCA AGCTATACTC CCGCTGGGCG 
GAGCGGCCGC GCCAGCGCAA CGATGGCATG CACCTGGACA ACCCGCACGC ACAGTTCCAC 
AATGCCACGC AACAGCTGCC CCAGGAATCG GCCATCAAGT TTCAGATCAT TCCTCCTCAA 
CAGCAGCAGC AGCAGCAACA ACAGACGGGA CATGGCGATG GCAGCTATCA GCAGCAATAT 
CGTGGTGCCA ATCAGATGCT GGCCGCAAGA AGCAATCTGG TGATGCAGCA GCAACAGCAA 
CAGCAGCAGC AATATCACCA GCAACAGCAG CAACACTTGA GTCTGCAACG ATTCGTGGCC 
AGTCAGAGCC AGAATTCGGC TCATTTGAAT CATCATCATC ATCGCCTGCA AATTGCCAAT 
ACAACAATCA CGGCCACAGC CAGAGATCCT TCCACAACAG TAGCAGCAGC AGCAACAGCA 
GCATCGGCAT CAGCAGCAGG AGGAGGCTCC GTCTTAAATG GAATGGATAC GGAACTAATT 
GAAATCCAAA GCCACATGAC TGCAAACAAA ATCACTAGCT CCGTCAGCAG CAATGGAAAT 
GGCAGCGGCA GCAATCCCAG TGCCAGTGCC AAGCAATTAG CAGCTCAAAC GAACAACGAA 
TTAAATGTCG TAAATAATAA CGACCCCAGC AACAATAACT TTATGTACAA TCAACAACAG 
CAGCAACTGA ATACTTCCAG CAACAGCAAC AACACACAGC AGCAGCAGCA ACATTATTAT 
AAATTGCAAC AGACATCAGC AACCCCAAAC AGTGGCCAAC TCCAACTCCA AAATCAAACC 
CAAACACAAA CACAAACCCC AACCCTTGTC CAACCCCCTG TTGAGGCCAT GTGTATGTCA 
CCTGAGCATC GCAGCGGCAG CAGCAGCAGC AGCAACAACA ATACGGCCTC AACGTCTTCC 
GCTGTCAACA GTCATAACAC CTCCCCCTCC ACAGCCATAG CCACAACCAC GTCCATGGCC 
GCATCTATAT CTATATCCAC ATCTACGTCC GGCACATCGT CCACCTCGAG TTCCTTACAA 
TCGATTATCG ATGGCAATCA GCAGCAACAA ATTACAACGA CATCGACGGC AGCAACTTGT 
GATAATTTAA TGAGCGCCAA TGCGCTCACT GAGCTGAATG GAGGGAGCAG CAGCAGCAGC 
AACAATCAGG CCACAGATGG CCACAACAAG AGTCAATCCC AATCGCAGTC TCAGCCGCAA 
ACTCTGAGCC AAAATGGTTG TGCAACATTC AGTGCTTCCA GTGATAATAG CAGCCAAATT 
AGCGCCAGCG ACGAGAGTAG CAGCTTTGGG GCCAACAATC AGAACAACAG CATACACAGC 
ATCCACAGCA GCCACAGCAG CAGCAGTAAC AGCAGCGAGG ACGAGCCCCA GGCGGGCAAT 
GCTGCCGAGA CCCTGAGCTT CTTCAACGAA ACCCTGGATC TATCCCATGA GGATATTCAA 
CGTACACTGA TAGCCAATAT GCAGCCGTAC AATACGGCTG CAGGCGAAGC AGCAGCGACA 
ACGATAGAAC CTTTGGCAAC AAACGGCAGC CACAGTAGCA GCAACAGCAA TGGCAATCCT 
CAAGAAGTGG AGAAGAAACC TTCAGTCGGC CAGGAAGTCG AAGCCGAACC TGAGGAGGAT 
GACACAGACG ATGTGTTTGC CAACCTGGAT GCCTTCGATA TGCTCGTGGA GTTCCCCGAG 
CTGGATCTGG ATGATAAGCA GGCCCTGAAC AACACGGCCT TGGAGCAGGG CTCCTATCTG 
GGACAGGCGG CCGCCCAAAC GCAGCAGCCC CGCAAGATCC ACAACATCTG CGACTTCAGT 
CCGGAGTGGT CGTACACCGA GGGCGGAGTC AAGGTACTTG TGGCCGGACC CTGGACGAGC 
AGCAATGGTG CTGGTGCCTA CACGGTGCTC TTCGATGCCC AGCCCGTGCC CACGCAGATG 
GTGCAGGAGG GAGTGCTCCG TTGCTACTGT CCTGCCCACG AGGCGGGCTT TGTGACGCTC 
CAGGTGGCCT GCGGCGGCTT TCTCGTGTCC AATTCGGTCA TGTTCGAGTA CAAGCTGTCG 
CTGCTGGCCG ATGCCCCGTT CGATGCGAGC AGCTCCAACG ATTGCCTGTA CAAATTCACG 
CTGCTCAATC GCCTCTCGAC CATCGACGAC AAGTTGCAGC TGAAGACGGA GCAGGAGCCG 
ACGACGGACC ACACGGCTCT CTATCTGGAG CCGAACTTTG AGGAGAAGCT GGTCGCGTAC 
TGCCACAGGC TGACGAAGCA TGCCTGGAGC ATGCCCAGCA CAGTGGCCAG CTGGAGCGTG 
GGCCTGCGGG GCATGACGCT GCTGCATCTG GCCGCCGCCT TGGGCTATGC CAAGCTGGTA 
GGCGCCATGC TCAATTGGCG GGCGGAGAAT CCACATATCA TACTCGAAAC GGAGCTGGAC 
GCCCTCAGTC AGGATGTGTA CGGTTTCACG CCGCTCGCCT GGTCCTGTGT GCGCGGCCAT 
GTGGAGTGTT CCCTGCTGCT CTACAAGTGG AATCACAATG CGCTGAAGAT CAAGACGCAG 
AGCCAGCAAA CGGCCCTGGA TTTGGCCAAC CTGAAGGGGC ACAAACATCT CCTGTCCCAG 
ATATTTCGAC TGGAGAAGGA GCGCTGCCGC AAGCCGCACT TGCGAGGCGG CCTGGCCAAT 
CTTTCAATGA ACCTAGGCGT TGAAACGGAA GCGGAGGAGT CGCCGCCGCA GACCTACAAC 
AGCTTTGACC TGGAGATACT CCAAAGAAAG CACGATGGCG TCTTCCTCAG ACCTGGGGCA 
GTGCATAGCA ATCAAAGTCC TCCCAACAAT GGCAGCCGGT ACTCCAAGCG CTCTTCCATA 
GACAGCGGCA TCAACATGGA CATACGCAGC AAGTCAGGCA AGTCCCTGGC CAGGCTGCAC 
AGCAACTTTG AGGGGCACGA CAGCTATGCA TTGGGAGTGG ACTCGCCGCT GGATACGCTG 
ACTGGGACCA ATTGCCTGCT CTCACCGCTA CGTAAAATGG ATTTCGCCCT GTGCGAGGTA 
TCCACGGGCG AGTCGAGCCC CATACACGAC AAAGACATCG ATGGCGATGA CACCTCGACG 
AGCGCCACCG ATGTGACCAT AGGCAATGAC CTGGCCCTGG CCCTGGCCGA TGCAGTTGTA 
GGGGACTCGG ATGCCAAAGT GCTGACTCTG GCCGAACACA TTATAGCAGC AATGCCAGAG 
CGCATCAAAA ATGAAGCCGA TGAAATGATG GTGCTGGGCA GCCCCTTGAC TGAGCCACTG 
ACCTCGGAGT CCTCGGCCCT GACAGACAGC TTTATGGATC CCCTGCTGGA CTCGCTGCCC 
AATACGCATT TCGACAGCGA CTTCAACTTC GACTTCCACG ACCACAGCTA TCGGTACCAC 
GATGTGAGCA CTCCCTGCTC CAGCCTGAGT CCCGCCAGCT CGGGGCCGCT GCAGTCGCCG 
GCCAGCTACT CCATACTGGG CACAGATCCG TCTGTCAGCT CGCCCAGTCC GCCGCCGTCG 
ACCAAGCAGC TGACCGAGTT CCTGCACGCA TCCAGCCTGT CGCAGTATCC GTTCGAGGCG 
GACTTCTCCA AGCTGACGCT GACCGACACG GAGCAGCGGG AGCTGTACGA GGCGGCCAAG 
TGCATACAGA AGGCCTACCG CTCGTACAAG GGCCGGCAGA AGCTCGAGGA GCAGAACAAA 
GAGCGCACTG CCGCCATTGT CATCCAGAAC TATTACAGGC GGTACAAGCA GTACGCCTAT 
TACAGGCAGA TGACCAACGC CGCCCTGGTC ATCCAGCATG GCTATCGTTC CTATCGAAAG 
AACAAACGCT TCAAGAAGAG CGGCCTGGGC CAGGGCATGG CAGGGGGCAG CGATCACGGC 
AGCGTCAGCA GCAACTCTCA GTGCCTGTCC AGCTTCTACG ACCACTACAA GCAGGATCAG 
CAGCAGCTGC AGCAGCAGCA TGAGATGGGC TCGCAGCCGA GCACGCCCAA GGAAACCAGT 
CCCTCGGGTC CCTTGAAACG AACCTACTCG CAGTCCACCC AGAATCAAGC TGCCAGGAAG 
ATCCAACAGT TCATGAGACA ATCACGCATC AAACTACAGA GAGAGCGAGC CGAAAAAGAG 
AAGCTGGTGC ACCAACGCAG GGCGGAATAC CTTCAAAACT TGCAGTATCA AGGGCAGCAG 
GAAATCTTGG CTTGCCTAGA AAATAACAGT TCTGCCCAGA GCAGTGGCAG CAACACCTAT 
ACGAACAGCA TCAACAATCT ACAATCCAAT CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004444391.1
CDS	371..6646
Translation	MENVSDASCSASSLKLQRQQTQIKAKNVLIRSQSSGKLSRNEAESTTTATTTATAKTTKATTRTLKIGAMQQKQLQPPNGVNASNIILLRGTRNENGQIIIQNKQDILTLLNEQQQHQEKAAQAAQASTAITLNQLPTTTTVARKTIVQSSGSSTTSTSSTISIVASSSSGSSSGVGAKDAASNTILLQTPINASQLESVLKAQERTKQANGTAQTKLIERPFLLKHATRSLSSESNCDSKSPFVLQTLKRLEKSQSILVIRNSTSGSTSSMAVSPPVNVSASVSSNVLSVTRTSKAAKGMAGALHKLKAAATAAAAAGAGAGAAPSASSTGTTILRLGKSATTLAINGAGGSSKLEVAAAAATATTTSTANKLSNTVTSEQQQQQQSTTTQTNVPLGNDGEPIKLPDNLESLPRADSFPSQRHRWNTNEEIAAILISFDKHCEWQSKEVRTRPKSGSLLLYSRKKVRYRRDGYCWKKRKDGKTTREDHMKLKVQGTECIYGCYVHSAILPTFHRRCYWLLQNPDIVLVHYLNVPYPDDNKMAVIAPSITLWGDKKEWTKEELVSQLKPMLSTVNSDDDSDSGNDIEISTAETVESIVCQLMEKQRLSRQAALVKQLDCGCGDSGCADGKTCTHPVMRRTGGIIKSAVATSASEKRLNEGAFNGGTPNVVVASKLYSRWAERPRQRNDGMHLDNPHAQFHNATQQLPQESAIKFQIIPPQQQQQQQQQTGHGDGSYQQQYRGANQMLAARSNLVMQQQQQQQQQYHQQQQQHLSLQRFVASQSQNSAHLNHHHHRLQIANTTITATARDPSTTVAAAATAASASAAGGGSVLNGMDTELIEIQSHMTANKITSSVSSNGNGSGSNPSASAKQLAAQTNNELNVVNNNDPSNNNFMYNQQQQQLNTSSNSNNTQQQQQHYYKLQQTSATPNSGQLQLQNQTQTQTQTPTLVQPPVEAMCMSPEHRSGSSSSSNNNTASTSSAVNSHNTSPSTAIATTTSMAASISISTSTSGTSSTSSSLQSIIDGNQQQQITTTSTAATCDNLMSANALTELNGGSSSSSNNQATDGHNKSQSQSQSQPQTLSQNGCATFSASSDNSSQISASDESSSFGANNQNNSIHSIHSSHSSSSNSSEDEPQAGNAAETLSFFNETLDLSHEDIQRTLIANMQPYNTAAGEAAATTIEPLATNGSHSSSNSNGNPQEVEKKPSVGQEVEAEPEEDDTDDVFANLDAFDMLVEFPELDLDDKQALNNTALEQGSYLGQAAAQTQQPRKIHNICDFSPEWSYTEGGVKVLVAGPWTSSNGAGAYTVLFDAQPVPTQMVQEGVLRCYCPAHEAGFVTLQVACGGFLVSNSVMFEYKLSLLADAPFDASSSNDCLYKFTLLNRLSTIDDKLQLKTEQEPTTDHTALYLEPNFEEKLVAYCHRLTKHAWSMPSTVASWSVGLRGMTLLHLAAALGYAKLVGAMLNWRAENPHIILETELDALSQDVYGFTPLAWSCVRGHVECSLLLYKWNHNALKIKTQSQQTALDLANLKGHKHLLSQIFRLEKERCRKPHLRGGLANLSMNLGVETEAEESPPQTYNSFDLEILQRKHDGVFLRPGAVHSNQSPPNNGSRYSKRSSIDSGINMDIRSKSGKSLARLHSNFEGHDSYALGVDSPLDTLTGTNCLLSPLRKMDFALCEVSTGESSPIHDKDIDGDDTSTSATDVTIGNDLALALADAVVGDSDAKVLTLAEHIIAAMPERIKNEADEMMVLGSPLTEPLTSESSALTDSFMDPLLDSLPNTHFDSDFNFDFHDHSYRYHDVSTPCSSLSPASSGPLQSPASYSILGTDPSVSSPSPPPSTKQLTEFLHASSLSQYPFEADFSKLTLTDTEQRELYEAAKCIQKAYRSYKGRQKLEEQNKERTAAIVIQNYYRRYKQYAYYRQMTNAALVIQHGYRSYRKNKRFKKSGLGQGMAGGSDHGSVSSNSQCLSSFYDHYKQDQQQLQQQHEMGSQPSTPKETSPSGPLKRTYSQSTQNQAARKIQQFMRQSRIKLQRERAEKEKLVHQRRAEYLQNLQYQGQQEILACLENNSSAQSSGSNTYTNSINNLQSNQ

Target ORF information:

RefSeq Version	XM_004444334.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA30460), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004444334.2

ORF Insert Sequence:

ATGGAAAATG TGTCCGATGC CAGCTGCAGC GCCTCTTCCC TGAAACTCCA ACGGCAGCAA 
ACTCAAATCA AAGCCAAAAA TGTCCTAATC AGATCCCAGA GCAGCGGCAA GCTTAGCAGA 
AATGAGGCTG AATCCACAAC AACAGCAACA ACAACAGCAA CAGCGAAAAC CACAAAAGCC 
ACAACGAGGA CTCTCAAGAT CGGGGCCATG CAACAGAAGC AGCTACAGCC ACCAAATGGA 
GTCAACGCTA GCAACATCAT CCTGCTGCGG GGCACACGCA ACGAGAACGG CCAGATCATT 
ATCCAGAACA AGCAGGACAT CCTCACCCTG CTCAACGAGC AGCAGCAGCA CCAGGAGAAG 
GCAGCCCAGG CGGCCCAGGC GTCCACAGCC ATCACACTCA ATCAACTGCC AACGACCACC 
ACAGTGGCTC GCAAGACGAT TGTCCAGAGC TCGGGGAGCA GCACCACCAG CACCAGCTCC 
ACCATATCCA TCGTGGCCAG CAGCAGCAGC GGCAGCAGTA GCGGTGTTGG TGCCAAGGAT 
GCCGCTAGCA ACACGATCCT CCTGCAGACG CCCATCAATG CCAGCCAGCT GGAGAGCGTG 
CTGAAGGCCC AGGAGCGAAC GAAACAGGCG AACGGCACGG CACAGACCAA GCTCATAGAG 
AGGCCCTTCC TGCTGAAGCA CGCCACGCGG AGCCTCAGCT CGGAGAGTAA TTGTGACAGC 
AAATCGCCGT TCGTGCTGCA GACACTCAAG CGACTGGAGA AATCGCAATC CATTCTGGTC 
ATACGCAACT CCACATCAGG CAGCACTTCC TCAATGGCCG TTTCTCCGCC TGTGAATGTT 
TCGGCCTCCG TATCATCGAA TGTTTTAAGC GTGACACGCA CTTCAAAGGC GGCCAAGGGC 
ATGGCAGGTG CGCTGCACAA GCTCAAGGCG GCCGCAACGG CAGCAGCCGC AGCGGGAGCA 
GGAGCAGGGG CAGCACCATC CGCATCATCG ACAGGCACAA CCATACTGAG GCTAGGCAAA 
AGCGCCACCA CATTGGCCAT TAACGGAGCT GGGGGCAGCA GCAAACTGGA GGTCGCCGCC 
GCCGCCGCAA CAGCAACCAC AACGTCAACA GCAAATAAAT TGTCCAACAC GGTGACATCG 
GAGCAGCAGC AACAGCAACA ATCGACAACA ACGCAAACGA ATGTGCCACT TGGGAACGAT 
GGCGAACCTA TCAAACTACC CGACAATTTG GAGAGCCTGC CAAGAGCTGA CAGTTTTCCA 
TCACAGCGTC ATCGTTGGAA TACAAATGAG GAAATTGCTG CGATTCTTAT CAGCTTTGAC 
AAGCACTGCG AGTGGCAGTC CAAGGAGGTC CGAACCAGAC CCAAGAGCGG ATCCCTGCTG 
CTGTACTCGC GGAAGAAGGT GCGCTATCGC CGCGACGGCT ATTGCTGGAA GAAGCGCAAG 
GATGGCAAGA CGACGCGCGA GGATCACATG AAACTGAAAG TACAAGGCAC GGAGTGCATC 
TACGGCTGTT ATGTACACTC GGCCATATTG CCCACGTTCC ATCGCAGATG TTACTGGCTG 
TTGCAGAATC CGGACATTGT TTTGGTGCAC TATCTGAATG TGCCGTATCC GGATGATAAT 
AAAATGGCAG TGATTGCCCC CAGCATCACC CTGTGGGGCG ACAAGAAAGA GTGGACCAAG 
GAGGAGCTGG TCAGCCAGCT GAAGCCGATG CTCTCCACAG TCAACTCCGA TGATGACTCC 
GACTCGGGCA ACGACATTGA GATATCGACG GCCGAGACCG TGGAGTCGAT TGTGTGCCAG 
CTGATGGAGA AGCAGCGCTT GTCCCGCCAG GCGGCCCTCG TCAAGCAGCT CGACTGCGGC 
TGCGGGGACT CGGGCTGTGC CGATGGCAAG ACCTGCACGC ATCCCGTGAT GCGGCGCACG 
GGCGGCATCA TCAAGTCCGC GGTGGCCACA TCGGCCTCAG AGAAGCGACT CAACGAGGGG 
GCCTTCAACG GCGGCACGCC CAATGTGGTG GTGGCCAGCA AGCTATACTC CCGCTGGGCG 
GAGCGGCCGC GCCAGCGCAA CGATGGCATG CACCTGGACA ACCCGCACGC ACAGTTCCAC 
AATGCCACGC AACAGCTGCC CCAGGAATCG GCCATCAAGT TTCAGATCAT TCCTCCTCAA 
CAGCAGCAGC AGCAGCAACA ACAGACGGGA CATGGCGATG GCAGCTATCA GCAGCAATAT 
CGTGGTGCCA ATCAGATGCT GGCCGCAAGA AGCAATCTGG TGATGCAGCA GCAACAGCAA 
CAGCAGCAGC AATATCACCA GCAACAGCAG CAACACTTGA GTCTGCAACG ATTCGTGGCC 
AGTCAGAGCC AGAATTCGGC TCATTTGAAT CATCATCATC ATCGCCTGCA AATTGCCAAT 
ACAACAATCA CGGCCACAGC CAGAGATCCT TCCACAACAG TAGCAGCAGC AGCAACAGCA 
GCATCGGCAT CAGCAGCAGG AGGAGGCTCC GTCTTAAATG GAATGGATAC GGAACTAATT 
GAAATCCAAA GCCACATGAC TGCAAACAAA ATCACTAGCT CCGTCAGCAG CAATGGAAAT 
GGCAGCGGCA GCAATCCCAG TGCCAGTGCC AAGCAATTAG CAGCTCAAAC GAACAACGAA 
TTAAATGTCG TAAATAATAA CGACCCCAGC AACAATAACT TTATGTACAA TCAACAACAG 
CAGCAACTGA ATACTTCCAG CAACAGCAAC AACACACAGC AGCAGCAGCA ACATTATTAT 
AAATTGCAAC AGACATCAGC AACCCCAAAC AGTGGCCAAC TCCAACTCCA AAATCAAACC 
CAAACACAAA CACAAACCCC AACCCTTGTC CAACCCCCTG TTGAGGCCAT GTGTATGTCA 
CCTGAGCATC GCAGCGGCAG CAGCAGCAGC AGCAACAACA ATACGGCCTC AACGTCTTCC 
GCTGTCAACA GTCATAACAC CTCCCCCTCC ACAGCCATAG CCACAACCAC GTCCATGGCC 
GCATCTATAT CTATATCCAC ATCTACGTCC GGCACATCGT CCACCTCGAG TTCCTTACAA 
TCGATTATCG ATGGCAATCA GCAGCAACAA ATTACAACGA CATCGACGGC AGCAACTTGT 
GATAATTTAA TGAGCGCCAA TGCGCTCACT GAGCTGAATG GAGGGAGCAG CAGCAGCAGC 
AACAATCAGG CCACAGATGG CCACAACAAG AGTCAATCCC AATCGCAGTC TCAGCCGCAA 
ACTCTGAGCC AAAATGGTTG TGCAACATTC AGTGCTTCCA GTGATAATAG CAGCCAAATT 
AGCGCCAGCG ACGAGAGTAG CAGCTTTGGG GCCAACAATC AGAACAACAG CATACACAGC 
ATCCACAGCA GCCACAGCAG CAGCAGTAAC AGCAGCGAGG ACGAGCCCCA GGCGGGCAAT 
GCTGCCGAGA CCCTGAGCTT CTTCAACGAA ACCCTGGATC TATCCCATGA GGATATTCAA 
CGTACACTGA TAGCCAATAT GCAGCCGTAC AATACGGCTG CAGGCGAAGC AGCAGCGACA 
ACGATAGAAC CTTTGGCAAC AAACGGCAGC CACAGTAGCA GCAACAGCAA TGGCAATCCT 
CAAGAAGTGG AGAAGAAACC TTCAGTCGGC CAGGAAGTCG AAGCCGAACC TGAGGAGGAT 
GACACAGACG ATGTGTTTGC CAACCTGGAT GCCTTCGATA TGCTCGTGGA GTTCCCCGAG 
CTGGATCTGG ATGATAAGCA GGCCCTGAAC AACACGGCCT TGGAGCAGGG CTCCTATCTG 
GGACAGGCGG CCGCCCAAAC GCAGCAGCCC CGCAAGATCC ACAACATCTG CGACTTCAGT 
CCGGAGTGGT CGTACACCGA GGGCGGAGTC AAGGTACTTG TGGCCGGACC CTGGACGAGC 
AGCAATGGTG CTGGTGCCTA CACGGTGCTC TTCGATGCCC AGCCCGTGCC CACGCAGATG 
GTGCAGGAGG GAGTGCTCCG TTGCTACTGT CCTGCCCACG AGGCGGGCTT TGTGACGCTC 
CAGGTGGCCT GCGGCGGCTT TCTCGTGTCC AATTCGGTCA TGTTCGAGTA CAAGCTGTCG 
CTGCTGGCCG ATGCCCCGTT CGATGCGAGC AGCTCCAACG ATTGCCTGTA CAAATTCACG 
CTGCTCAATC GCCTCTCGAC CATCGACGAC AAGTTGCAGC TGAAGACGGA GCAGGAGCCG 
ACGACGGACC ACACGGCTCT CTATCTGGAG CCGAACTTTG AGGAGAAGCT GGTCGCGTAC 
TGCCACAGGC TGACGAAGCA TGCCTGGAGC ATGCCCAGCA CAGTGGCCAG CTGGAGCGTG 
GGCCTGCGGG GCATGACGCT GCTGCATCTG GCCGCCGCCT TGGGCTATGC CAAGCTGGTA 
GGCGCCATGC TCAATTGGCG GGCGGAGAAT CCACATATCA TACTCGAAAC GGAGCTGGAC 
GCCCTCAGTC AGGATGTGTA CGGTTTCACG CCGCTCGCCT GGTCCTGTGT GCGCGGCCAT 
GTGGAGTGTT CCCTGCTGCT CTACAAGTGG AATCACAATG CGCTGAAGAT CAAGACGCAG 
AGCCAGCAAA CGGCCCTGGA TTTGGCCAAC CTGAAGGGGC ACAAACATCT CCTGTCCCAG 
ATATTTCGAC TGGAGAAGGA GCGCTGCCGC AAGCCGCACT TGCGAGGCGG CCTGGCCAAT 
CTTTCAATGA ACCTAGGCGT TGAAACGGAA GCGGAGGAGT CGCCGCCGCA GACCTACAAC 
AGCTTTGACC TGGAGATACT CCAAAGAAAG CACGATGGCG TCTTCCTCAG ACCTGGGGCA 
GTGCATAGCA ATCAAAGTCC TCCCAACAAT GGCAGCCGGT ACTCCAAGCG CTCTTCCATA 
GACAGCGGCA TCAACATGGA CATACGCAGC AAGTCAGGCA AGTCCCTGGC CAGGCTGCAC 
AGCAACTTTG AGGGGCACGA CAGCTATGCA TTGGGAGTGG ACTCGCCGCT GGATACGCTG 
ACTGGGACCA ATTGCCTGCT CTCACCGCTA CGTAAAATGG ATTTCGCCCT GTGCGAGGTA 
TCCACGGGCG AGTCGAGCCC CATACACGAC AAAGACATCG ATGGCGATGA CACCTCGACG 
AGCGCCACCG ATGTGACCAT AGGCAATGAC CTGGCCCTGG CCCTGGCCGA TGCAGTTGTA 
GGGGACTCGG ATGCCAAAGT GCTGACTCTG GCCGAACACA TTATAGCAGC AATGCCAGAG 
CGCATCAAAA ATGAAGCCGA TGAAATGATG GTGCTGGGCA GCCCCTTGAC TGAGCCACTG 
ACCTCGGAGT CCTCGGCCCT GACAGACAGC TTTATGGATC CCCTGCTGGA CTCGCTGCCC 
AATACGCATT TCGACAGCGA CTTCAACTTC GACTTCCACG ACCACAGCTA TCGGTACCAC 
GATGTGAGCA CTCCCTGCTC CAGCCTGAGT CCCGCCAGCT CGGGGCCGCT GCAGTCGCCG 
GCCAGCTACT CCATACTGGG CACAGATCCG TCTGTCAGCT CGCCCAGTCC GCCGCCGTCG 
ACCAAGCAGC TGACCGAGTT CCTGCACGCA TCCAGCCTGT CGCAGTATCC GTTCGAGGCG 
GACTTCTCCA AGCTGACGCT GACCGACACG GAGCAGCGGG AGCTGTACGA GGCGGCCAAG 
TGCATACAGA AGGCCTACCG CTCGTACAAG GGCCGGCAGA AGCTCGAGGA GCAGAACAAA 
GAGCGCACTG CCGCCATTGT CATCCAGAAC TATTACAGGC GGTACAAGCA GTACGCCTAT 
TACAGGCAGA TGACCAACGC CGCCCTGGTC ATCCAGCATG GCTATCGTTC CTATCGAAAG 
AACAAACGCT TCAAGAAGAG CGGCCTGGGC CAGGGCATGG CAGGGGGCAG CGATCACGGC 
AGCGTCAGCA GCAACTCTCA GTGCCTGTCC AGCTTCTACG ACCACTACAA GCAGGATCAG 
CAGCAGCTGC AGCAGCAGCA TGAGATGGGC TCGCAGCCGA GCACGCCCAA GGAAACCAGT 
CCCTCGGGTC CCTTGAAACG AACCTACTCG CAGTCCACCC AGAATCAAGC TGCCAGGAAG 
ATCCAACAGT TCATGAGACA ATCACGCATC AAACTACAGA GAGAGCGAGC CGAAAAAGAG 
AAGCTGGTGC ACCAACGCAG GGCGGAATAC CTTCAAAACT TGCAGTATCA AGGGCAGCAG 
GAAATCTTGG CTTGCCTAGA AAATAACAGT TCTGCCCAGA GCAGTGGCAG CAACACCTAT 
ACGAACAGCA TCAACAATCT ACAATCCAAT CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA30460 ( XM_015184948.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015040434.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA30460), mRNA.

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CloneID	ODe23355
Clone ID Related Accession (Same CDS sequence)	XM_015184948.1
Accession Version	XM_015184948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAATG TGTCCGATGC CAGCTGCAGC GCCTCTTCCC TGAAACTCCA ACGGCAGCAA 
ACTCAAATCA AAGCCAAAAA TGTCCTAATC AGATCCCAGA GCAGCGGCAA GCTTAGCAGA 
AATGAGGCTG AATCCACAAC AACAGCAACA ACAACAGCAA CAGCGAAAAC CACAAAAGCC 
ACAACGAGGA CTCTCAAGAT CGGGGCCATG CAACAGAAGC AGCTACAGCC ACCAAATGGA 
GTCAACGCTA GCAACATCAT CCTGCTGCGG GGCACACGCA ACGAGAACGG CCAGATCATT 
ATCCAGAACA AGCAGGACAT CCTCACCCTG CTCAACGAGC AGCAGCAGCA CCAGGAGAAG 
GCAGCCCAGG CGGCCCAGGC GTCCACAGCC ATCACACTCA ATCAACTGCC AACGACCACC 
ACAGTGGCTC GCAAGACGAT TGTCCAGAGC TCGGGGAGCA GCACCACCAG CACCAGCTCC 
ACCATATCCA TCGTGGCCAG CAGCAGCAGC GGCAGCAGTA GCGGTGTTGG TGCCAAGGAT 
GCCGCTAGCA ACACGATCCT CCTGCAGACG CCCATCAATG CCAGCCAGCT GGAGAGCGTG 
CTGAAGGCCC AGGAGCGAAC GAAACAGGCG AACGGCACGG CACAGACCAA GCTCATAGAG 
AGGCCCTTCC TGCTGAAGCA CGCCACGCGG AGCCTCAGCT CGGAGAGTAA TTGTGACAGC 
AAATCGCCGT TCGTGCTGCA GACACTCAAG CGACTGGAGA AATCGCAATC CATTCTGGTC 
ATACGCAACT CCACATCAGG CAGCACTTCC TCAATGGCCG TTTCTCCGCC TGTGAATGTT 
TCGGCCTCCG TATCATCGAA TGTTTTAAGC GTGACACGCA CTTCAAAGGC GGCCAAGGGC 
ATGGCAGGTG CGCTGCACAA GCTCAAGGCG GCCGCAACGG CAGCAGCCGC AGCGGGAGCA 
GGAGCAGGGG CAGCACCATC CGCATCATCG ACAGGCACAA CCATACTGAG GCTAGGCAAA 
AGCGCCACCA CATTGGCCAT TAACGGAGCT GGGGGCAGCA GCAAACTGGA GGTCGCCGCC 
GCCGCCGCAA CAGCAACCAC AACGTCAACA GCAAATAAAT TGTCCAACAC GGTGACATCG 
GAGCAGCAGC AACAGCAACA ATCGACAACA ACGCAAACGA ATGTGCCACT TGGGAACGAT 
GGCGAACCTA TCAAACTACC CGACAATTTG GAGAGCCTGC CAAGAGCTGA CAGTTTTCCA 
TCACAGCGTC ATCGTTGGAA TACAAATGAG GAAATTGCTG CGATTCTTAT CAGCTTTGAC 
AAGCACTGCG AGTGGCAGTC CAAGGAGGTC CGAACCAGAC CCAAGAGCGG ATCCCTGCTG 
CTGTACTCGC GGAAGAAGGT GCGCTATCGC CGCGACGGCT ATTGCTGGAA GAAGCGCAAG 
GATGGCAAGA CGACGCGCGA GGATCACATG AAACTGAAAG TACAAGGCAC GGAGTGCATC 
TACGGCTGTT ATGTACACTC GGCCATATTG CCCACGTTCC ATCGCAGATG TTACTGGCTG 
TTGCAGAATC CGGACATTGT TTTGGTGCAC TATCTGAATG TGCCGTATCC GGATGATAAT 
AAAATGGCAG TGATTGCCCC CAGCATCACC CTGTGGGGCG ACAAGAAAGA GTGGACCAAG 
GAGGAGCTGG TCAGCCAGCT GAAGCCGATG CTCTCCACAG TCAACTCCGA TGATGACTCC 
GACTCGGGCA ACGACATTGA GATATCGACG GCCGAGACCG TGGAGTCGAT TGTGTGCCAG 
CTGATGGAGA AGCAGCGCTT GTCCCGCCAG GCGGCCCTCG TCAAGCAGCT CGACTGCGGC 
TGCGGGGACT CGGGCTGTGC CGATGGCAAG ACCTGCACGC ATCCCGTGAT GCGGCGCACG 
GGCGGCATCA TCAAGTCCGC GGTGGCCACA TCGGCCTCAG AGAAGCGACT CAACGAGGGG 
GCCTTCAACG GCGGCACGCC CAATGTGGTG GTGGCCAGCA AGCTATACTC CCGCTGGGCG 
GAGCGGCCGC GCCAGCGCAA CGATGGCATG CACCTGGACA ACCCGCACGC ACAGTTCCAC 
AATGCCACGC AACAGCTGCC CCAGGAATCG GCCATCAAGT TTCAGATCAT TCCTCCTCAA 
CAGCAGCAGC AGCAGCAACA ACAGACGGGA CATGGCGATG GCAGCTATCA GCAGCAATAT 
CGTGGTGCCA ATCAGATGCT GGCCGCAAGA AGCAATCTGG TGATGCAGCA GCAACAGCAA 
CAGCAGCAGC AATATCACCA GCAACAGCAG CAACACTTGA GTCTGCAACG ATTCGTGGCC 
AGTCAGAGCC AGAATTCGGC TCATTTGAAT CATCATCATC ATCGCCTGCA AATTGCCAAT 
ACAACAATCA CGGCCACAGC CAGAGATCCT TCCACAACAG TAGCAGCAGC AGCAACAGCA 
GCATCGGCAT CAGCAGCAGG AGGAGGCTCC GTCTTAAATG GAATGGATAC GGAACTAATT 
GAAATCCAAA GCCACATGAC TGCAAACAAA ATCACTAGCT CCGTCAGCAG CAATGGAAAT 
GGCAGCGGCA GCAATCCCAG TGCCAGTGCC AAGCAATTAG CAGCTCAAAC GAACAACGAA 
TTAAATGTCG TAAATAATAA CGACCCCAGC AACAATAACT TTATGTACAA TCAACAACAG 
CAGCAACTGA ATACTTCCAG CAACAGCAAC AACACACAGC AGCAGCAGCA ACATTATTAT 
AAATTGCAAC AGACATCAGC AACCCCAAAC AGTGGCCAAC TCCAACTCCA AAATCAAACC 
CAAACACAAA CACAAACCCC AACCCTTGTC CAACCCCCTG TTGAGGCCAT GTGTATGTCA 
CCTGAGCATC GCAGCGGCAG CAGCAGCAGC AGCAACAACA ATACGGCCTC AACGTCTTCC 
GCTGTCAACA GTCATAACAC CTCCCCCTCC ACAGCCATAG CCACAACCAC GTCCATGGCC 
GCATCTATAT CTATATCCAC ATCTACGTCC GGCACATCGT CCACCTCGAG TTCCTTACAA 
TCGATTATCG ATGGCAATCA GCAGCAACAA ATTACAACGA CATCGACGGC AGCAACTTGT 
GATAATTTAA TGAGCGCCAA TGCGCTCACT GAGCTGAATG GAGGGAGCAG CAGCAGCAGC 
AACAATCAGG CCACAGATGG CCACAACAAG AGTCAATCCC AATCGCAGTC TCAGCCGCAA 
ACTCTGAGCC AAAATGGTTG TGCAACATTC AGTGCTTCCA GTGATAATAG CAGCCAAATT 
AGCGCCAGCG ACGAGAGTAG CAGCTTTGGG GCCAACAATC AGAACAACAG CATACACAGC 
ATCCACAGCA GCCACAGCAG CAGCAGTAAC AGCAGCGAGG ACGAGCCCCA GGCGGGCAAT 
GCTGCCGAGA CCCTGAGCTT CTTCAACGAA ACCCTGGATC TATCCCATGA GGATATTCAA 
CGTACACTGA TAGCCAATAT GCAGCCGTAC AATACGGCTG CAGGCGAAGC AGCAGCGACA 
ACGATAGAAC CTTTGGCAAC AAACGGCAGC CACAGTAGCA GCAACAGCAA TGGCAATCCT 
CAAGAAGTGG AGAAGAAACC TTCAGTCGGC CAGGAAGTCG AAGCCGAACC TGAGGAGGAT 
GACACAGACG ATGTGTTTGC CAACCTGGAT GCCTTCGATA TGCTCGTGGA GTTCCCCGAG 
CTGGATCTGG ATGATAAGCA GGCCCTGAAC AACACGGCCT TGGAGCAGGG CTCCTATCTG 
GGACAGGCGG CCGCCCAAAC GCAGCAGCCC CGCAAGATCC ACAACATCTG CGACTTCAGT 
CCGGAGTGGT CGTACACCGA GGGCGGAGTC AAGGTACTTG TGGCCGGACC CTGGACGAGC 
AGCAATGGTG CTGGTGCCTA CACGGTGCTC TTCGATGCCC AGCCCGTGCC CACGCAGATG 
GTGCAGGAGG GAGTGCTCCG TTGCTACTGT CCTGCCCACG AGGCGGGCTT TGTGACGCTC 
CAGGTGGCCT GCGGCGGCTT TCTCGTGTCC AATTCGGTCA TGTTCGAGTA CAAGCTGTCG 
CTGCTGGCCG ATGCCCCGTT CGATGCGAGC AGCTCCAACG ATTGCCTGTA CAAATTCACG 
CTGCTCAATC GCCTCTCGAC CATCGACGAC AAGTTGCAGC TGAAGACGGA GCAGGAGCCG 
ACGACGGACC ACACGGCTCT CTATCTGGAG CCGAACTTTG AGGAGAAGCT GGTCGCGTAC 
TGCCACAGGC TGACGAAGCA TGCCTGGAGC ATGCCCAGCA CAGTGGCCAG CTGGAGCGTG 
GGCCTGCGGG GCATGACGCT GCTGCATCTG GCCGCCGCCT TGGGCTATGC CAAGCTGGTA 
GGCGCCATGC TCAATTGGCG GGCGGAGAAT CCACATATCA TACTCGAAAC GGAGCTGGAC 
GCCCTCAGTC AGGATGTGTA CGGTTTCACG CCGCTCGCCT GGTCCTGTGT GCGCGGCCAT 
GTGGAGTGTT CCCTGCTGCT CTACAAGTGG AATCACAATG CGCTGAAGAT CAAGACGCAG 
AGCCAGCAAA CGGCCCTGGA TTTGGCCAAC CTGAAGGGGC ACAAACATCT CCTGTCCCAG 
ATATTTCGAC TGGAGAAGGA GCGCTGCCGC AAGCCGCACT TGCGAGGCGG CCTGGCCAAT 
CTTTCAATGA ACCTAGGCGT TGAAACGGAA GCGGAGGAGT CGCCGCCGCA GACCTACAAC 
AGCTTTGACC TGGAGATACT CCAAAGAAAG CACGATGGCG TCTTCCTCAG ACCTGGGGCA 
GTGCATAGCA ATCAAAGTCC TCCCAACAAT GGCAGCCGGT ACTCCAAGCG CTCTTCCATA 
GACAGCGGCA TCAACATGGA CATACGCAGC AAGTCAGGCA AGTCCCTGGC CAGGCTGCAC 
AGCAACTTTG AGGGGCACGA CAGCTATGCA TTGGGAGTGG ACTCGCCGCT GGATACGCTG 
ACTGGGACCA ATTGCCTGCT CTCACCGCTA CGTAAAATGG ATTTCGCCCT GTGCGAGGTA 
TCCACGGGCG AGTCGAGCCC CATACACGAC AAAGACATCG ATGGCGATGA CACCTCGACG 
AGCGCCACCG ATGTGACCAT AGGCAATGAC CTGGCCCTGG CCCTGGCCGA TGCAGTTGTA 
GGGGACTCGG ATGCCAAAGT GCTGACTCTG GCCGAACACA TTATAGCAGC AATGCCAGAG 
CGCATCAAAA ATGAAGCCGA TGAAATGATG GTGCTGGGCA GCCCCTTGAC TGAGCCACTG 
ACCTCGGAGT CCTCGGCCCT GACAGACAGC TTTATGGATC CCCTGCTGGA CTCGCTGCCC 
AATACGCATT TCGACAGCGA CTTCAACTTC GACTTCCACG ACCACAGCTA TCGGTACCAC 
GATGTGAGCA CTCCCTGCTC CAGCCTGAGT CCCGCCAGCT CGGGGCCGCT GCAGTCGCCG 
GCCAGCTACT CCATACTGGG CACAGATCCG TCTGTCAGCT CGCCCAGTCC GCCGCCGTCG 
ACCAAGCAGC TGACCGAGTT CCTGCACGCA TCCAGCCTGT CGCAGTATCC GTTCGAGGCG 
GACTTCTCCA AGCTGACGCT GACCGACACG GAGCAGCGGG AGCTGTACGA GGCGGCCAAG 
TGCATACAGA AGGCCTACCG CTCGTACAAG GGCCGGCAGA AGCTCGAGGA GCAGAACAAA 
GAGCGCACTG CCGCCATTGT CATCCAGAAC TATTACAGGC GGTACAAGCA GTACGCCTAT 
TACAGGCAGA TGACCAACGC CGCCCTGGTC ATCCAGCATG GCTATCGTTC CTATCGAAAG 
AACAAACGCT TCAAGAAGAG CGGCCTGGGC CAGGGCATGG CAGGGGGCAG CGATCACGGC 
AGCGTCAGCA GCAACTCTCA GTGCCTGTCC AGCTTCTACG ACCACTACAA GCAGGATCAG 
CAGCAGCTGC AGCAGCAGCA TGAGATGGGC TCGCAGCCGA GCACGCCCAA GGAAACCAGT 
CCCTCGGGTC CCTTGAAACG AACCTACTCG CAGTCCACCC AGAATCAAGC TGCCAGGAAG 
ATCCAACAGT TCATGAGACA ATCACGCATC AAACTACAGA GAGAGCGAGC CGAAAAAGAG 
AAGCTGGTGC ACCAACGCAG GGCGGAATAC CTTCAAAACT TGCAGTATCA AGGGCAGCAG 
GAAATCTTGG CTTGCCTAGA AAATAATTCT GCCCAGAGCA GTGGCAGCAA CACCTATACG 
AACAGCATCA ACAATCTACA ATCCAATCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015040434.1
CDS	371..6643
Translation	MENVSDASCSASSLKLQRQQTQIKAKNVLIRSQSSGKLSRNEAESTTTATTTATAKTTKATTRTLKIGAMQQKQLQPPNGVNASNIILLRGTRNENGQIIIQNKQDILTLLNEQQQHQEKAAQAAQASTAITLNQLPTTTTVARKTIVQSSGSSTTSTSSTISIVASSSSGSSSGVGAKDAASNTILLQTPINASQLESVLKAQERTKQANGTAQTKLIERPFLLKHATRSLSSESNCDSKSPFVLQTLKRLEKSQSILVIRNSTSGSTSSMAVSPPVNVSASVSSNVLSVTRTSKAAKGMAGALHKLKAAATAAAAAGAGAGAAPSASSTGTTILRLGKSATTLAINGAGGSSKLEVAAAAATATTTSTANKLSNTVTSEQQQQQQSTTTQTNVPLGNDGEPIKLPDNLESLPRADSFPSQRHRWNTNEEIAAILISFDKHCEWQSKEVRTRPKSGSLLLYSRKKVRYRRDGYCWKKRKDGKTTREDHMKLKVQGTECIYGCYVHSAILPTFHRRCYWLLQNPDIVLVHYLNVPYPDDNKMAVIAPSITLWGDKKEWTKEELVSQLKPMLSTVNSDDDSDSGNDIEISTAETVESIVCQLMEKQRLSRQAALVKQLDCGCGDSGCADGKTCTHPVMRRTGGIIKSAVATSASEKRLNEGAFNGGTPNVVVASKLYSRWAERPRQRNDGMHLDNPHAQFHNATQQLPQESAIKFQIIPPQQQQQQQQQTGHGDGSYQQQYRGANQMLAARSNLVMQQQQQQQQQYHQQQQQHLSLQRFVASQSQNSAHLNHHHHRLQIANTTITATARDPSTTVAAAATAASASAAGGGSVLNGMDTELIEIQSHMTANKITSSVSSNGNGSGSNPSASAKQLAAQTNNELNVVNNNDPSNNNFMYNQQQQQLNTSSNSNNTQQQQQHYYKLQQTSATPNSGQLQLQNQTQTQTQTPTLVQPPVEAMCMSPEHRSGSSSSSNNNTASTSSAVNSHNTSPSTAIATTTSMAASISISTSTSGTSSTSSSLQSIIDGNQQQQITTTSTAATCDNLMSANALTELNGGSSSSSNNQATDGHNKSQSQSQSQPQTLSQNGCATFSASSDNSSQISASDESSSFGANNQNNSIHSIHSSHSSSSNSSEDEPQAGNAAETLSFFNETLDLSHEDIQRTLIANMQPYNTAAGEAAATTIEPLATNGSHSSSNSNGNPQEVEKKPSVGQEVEAEPEEDDTDDVFANLDAFDMLVEFPELDLDDKQALNNTALEQGSYLGQAAAQTQQPRKIHNICDFSPEWSYTEGGVKVLVAGPWTSSNGAGAYTVLFDAQPVPTQMVQEGVLRCYCPAHEAGFVTLQVACGGFLVSNSVMFEYKLSLLADAPFDASSSNDCLYKFTLLNRLSTIDDKLQLKTEQEPTTDHTALYLEPNFEEKLVAYCHRLTKHAWSMPSTVASWSVGLRGMTLLHLAAALGYAKLVGAMLNWRAENPHIILETELDALSQDVYGFTPLAWSCVRGHVECSLLLYKWNHNALKIKTQSQQTALDLANLKGHKHLLSQIFRLEKERCRKPHLRGGLANLSMNLGVETEAEESPPQTYNSFDLEILQRKHDGVFLRPGAVHSNQSPPNNGSRYSKRSSIDSGINMDIRSKSGKSLARLHSNFEGHDSYALGVDSPLDTLTGTNCLLSPLRKMDFALCEVSTGESSPIHDKDIDGDDTSTSATDVTIGNDLALALADAVVGDSDAKVLTLAEHIIAAMPERIKNEADEMMVLGSPLTEPLTSESSALTDSFMDPLLDSLPNTHFDSDFNFDFHDHSYRYHDVSTPCSSLSPASSGPLQSPASYSILGTDPSVSSPSPPPSTKQLTEFLHASSLSQYPFEADFSKLTLTDTEQRELYEAAKCIQKAYRSYKGRQKLEEQNKERTAAIVIQNYYRRYKQYAYYRQMTNAALVIQHGYRSYRKNKRFKKSGLGQGMAGGSDHGSVSSNSQCLSSFYDHYKQDQQQLQQQHEMGSQPSTPKETSPSGPLKRTYSQSTQNQAARKIQQFMRQSRIKLQRERAEKEKLVHQRRAEYLQNLQYQGQQEILACLENNSAQSSGSNTYTNSINNLQSNQ

Target ORF information:

RefSeq Version	XM_015184948.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA30460), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015184948.1

ORF Insert Sequence:

ATGGAAAATG TGTCCGATGC CAGCTGCAGC GCCTCTTCCC TGAAACTCCA ACGGCAGCAA 
ACTCAAATCA AAGCCAAAAA TGTCCTAATC AGATCCCAGA GCAGCGGCAA GCTTAGCAGA 
AATGAGGCTG AATCCACAAC AACAGCAACA ACAACAGCAA CAGCGAAAAC CACAAAAGCC 
ACAACGAGGA CTCTCAAGAT CGGGGCCATG CAACAGAAGC AGCTACAGCC ACCAAATGGA 
GTCAACGCTA GCAACATCAT CCTGCTGCGG GGCACACGCA ACGAGAACGG CCAGATCATT 
ATCCAGAACA AGCAGGACAT CCTCACCCTG CTCAACGAGC AGCAGCAGCA CCAGGAGAAG 
GCAGCCCAGG CGGCCCAGGC GTCCACAGCC ATCACACTCA ATCAACTGCC AACGACCACC 
ACAGTGGCTC GCAAGACGAT TGTCCAGAGC TCGGGGAGCA GCACCACCAG CACCAGCTCC 
ACCATATCCA TCGTGGCCAG CAGCAGCAGC GGCAGCAGTA GCGGTGTTGG TGCCAAGGAT 
GCCGCTAGCA ACACGATCCT CCTGCAGACG CCCATCAATG CCAGCCAGCT GGAGAGCGTG 
CTGAAGGCCC AGGAGCGAAC GAAACAGGCG AACGGCACGG CACAGACCAA GCTCATAGAG 
AGGCCCTTCC TGCTGAAGCA CGCCACGCGG AGCCTCAGCT CGGAGAGTAA TTGTGACAGC 
AAATCGCCGT TCGTGCTGCA GACACTCAAG CGACTGGAGA AATCGCAATC CATTCTGGTC 
ATACGCAACT CCACATCAGG CAGCACTTCC TCAATGGCCG TTTCTCCGCC TGTGAATGTT 
TCGGCCTCCG TATCATCGAA TGTTTTAAGC GTGACACGCA CTTCAAAGGC GGCCAAGGGC 
ATGGCAGGTG CGCTGCACAA GCTCAAGGCG GCCGCAACGG CAGCAGCCGC AGCGGGAGCA 
GGAGCAGGGG CAGCACCATC CGCATCATCG ACAGGCACAA CCATACTGAG GCTAGGCAAA 
AGCGCCACCA CATTGGCCAT TAACGGAGCT GGGGGCAGCA GCAAACTGGA GGTCGCCGCC 
GCCGCCGCAA CAGCAACCAC AACGTCAACA GCAAATAAAT TGTCCAACAC GGTGACATCG 
GAGCAGCAGC AACAGCAACA ATCGACAACA ACGCAAACGA ATGTGCCACT TGGGAACGAT 
GGCGAACCTA TCAAACTACC CGACAATTTG GAGAGCCTGC CAAGAGCTGA CAGTTTTCCA 
TCACAGCGTC ATCGTTGGAA TACAAATGAG GAAATTGCTG CGATTCTTAT CAGCTTTGAC 
AAGCACTGCG AGTGGCAGTC CAAGGAGGTC CGAACCAGAC CCAAGAGCGG ATCCCTGCTG 
CTGTACTCGC GGAAGAAGGT GCGCTATCGC CGCGACGGCT ATTGCTGGAA GAAGCGCAAG 
GATGGCAAGA CGACGCGCGA GGATCACATG AAACTGAAAG TACAAGGCAC GGAGTGCATC 
TACGGCTGTT ATGTACACTC GGCCATATTG CCCACGTTCC ATCGCAGATG TTACTGGCTG 
TTGCAGAATC CGGACATTGT TTTGGTGCAC TATCTGAATG TGCCGTATCC GGATGATAAT 
AAAATGGCAG TGATTGCCCC CAGCATCACC CTGTGGGGCG ACAAGAAAGA GTGGACCAAG 
GAGGAGCTGG TCAGCCAGCT GAAGCCGATG CTCTCCACAG TCAACTCCGA TGATGACTCC 
GACTCGGGCA ACGACATTGA GATATCGACG GCCGAGACCG TGGAGTCGAT TGTGTGCCAG 
CTGATGGAGA AGCAGCGCTT GTCCCGCCAG GCGGCCCTCG TCAAGCAGCT CGACTGCGGC 
TGCGGGGACT CGGGCTGTGC CGATGGCAAG ACCTGCACGC ATCCCGTGAT GCGGCGCACG 
GGCGGCATCA TCAAGTCCGC GGTGGCCACA TCGGCCTCAG AGAAGCGACT CAACGAGGGG 
GCCTTCAACG GCGGCACGCC CAATGTGGTG GTGGCCAGCA AGCTATACTC CCGCTGGGCG 
GAGCGGCCGC GCCAGCGCAA CGATGGCATG CACCTGGACA ACCCGCACGC ACAGTTCCAC 
AATGCCACGC AACAGCTGCC CCAGGAATCG GCCATCAAGT TTCAGATCAT TCCTCCTCAA 
CAGCAGCAGC AGCAGCAACA ACAGACGGGA CATGGCGATG GCAGCTATCA GCAGCAATAT 
CGTGGTGCCA ATCAGATGCT GGCCGCAAGA AGCAATCTGG TGATGCAGCA GCAACAGCAA 
CAGCAGCAGC AATATCACCA GCAACAGCAG CAACACTTGA GTCTGCAACG ATTCGTGGCC 
AGTCAGAGCC AGAATTCGGC TCATTTGAAT CATCATCATC ATCGCCTGCA AATTGCCAAT 
ACAACAATCA CGGCCACAGC CAGAGATCCT TCCACAACAG TAGCAGCAGC AGCAACAGCA 
GCATCGGCAT CAGCAGCAGG AGGAGGCTCC GTCTTAAATG GAATGGATAC GGAACTAATT 
GAAATCCAAA GCCACATGAC TGCAAACAAA ATCACTAGCT CCGTCAGCAG CAATGGAAAT 
GGCAGCGGCA GCAATCCCAG TGCCAGTGCC AAGCAATTAG CAGCTCAAAC GAACAACGAA 
TTAAATGTCG TAAATAATAA CGACCCCAGC AACAATAACT TTATGTACAA TCAACAACAG 
CAGCAACTGA ATACTTCCAG CAACAGCAAC AACACACAGC AGCAGCAGCA ACATTATTAT 
AAATTGCAAC AGACATCAGC AACCCCAAAC AGTGGCCAAC TCCAACTCCA AAATCAAACC 
CAAACACAAA CACAAACCCC AACCCTTGTC CAACCCCCTG TTGAGGCCAT GTGTATGTCA 
CCTGAGCATC GCAGCGGCAG CAGCAGCAGC AGCAACAACA ATACGGCCTC AACGTCTTCC 
GCTGTCAACA GTCATAACAC CTCCCCCTCC ACAGCCATAG CCACAACCAC GTCCATGGCC 
GCATCTATAT CTATATCCAC ATCTACGTCC GGCACATCGT CCACCTCGAG TTCCTTACAA 
TCGATTATCG ATGGCAATCA GCAGCAACAA ATTACAACGA CATCGACGGC AGCAACTTGT 
GATAATTTAA TGAGCGCCAA TGCGCTCACT GAGCTGAATG GAGGGAGCAG CAGCAGCAGC 
AACAATCAGG CCACAGATGG CCACAACAAG AGTCAATCCC AATCGCAGTC TCAGCCGCAA 
ACTCTGAGCC AAAATGGTTG TGCAACATTC AGTGCTTCCA GTGATAATAG CAGCCAAATT 
AGCGCCAGCG ACGAGAGTAG CAGCTTTGGG GCCAACAATC AGAACAACAG CATACACAGC 
ATCCACAGCA GCCACAGCAG CAGCAGTAAC AGCAGCGAGG ACGAGCCCCA GGCGGGCAAT 
GCTGCCGAGA CCCTGAGCTT CTTCAACGAA ACCCTGGATC TATCCCATGA GGATATTCAA 
CGTACACTGA TAGCCAATAT GCAGCCGTAC AATACGGCTG CAGGCGAAGC AGCAGCGACA 
ACGATAGAAC CTTTGGCAAC AAACGGCAGC CACAGTAGCA GCAACAGCAA TGGCAATCCT 
CAAGAAGTGG AGAAGAAACC TTCAGTCGGC CAGGAAGTCG AAGCCGAACC TGAGGAGGAT 
GACACAGACG ATGTGTTTGC CAACCTGGAT GCCTTCGATA TGCTCGTGGA GTTCCCCGAG 
CTGGATCTGG ATGATAAGCA GGCCCTGAAC AACACGGCCT TGGAGCAGGG CTCCTATCTG 
GGACAGGCGG CCGCCCAAAC GCAGCAGCCC CGCAAGATCC ACAACATCTG CGACTTCAGT 
CCGGAGTGGT CGTACACCGA GGGCGGAGTC AAGGTACTTG TGGCCGGACC CTGGACGAGC 
AGCAATGGTG CTGGTGCCTA CACGGTGCTC TTCGATGCCC AGCCCGTGCC CACGCAGATG 
GTGCAGGAGG GAGTGCTCCG TTGCTACTGT CCTGCCCACG AGGCGGGCTT TGTGACGCTC 
CAGGTGGCCT GCGGCGGCTT TCTCGTGTCC AATTCGGTCA TGTTCGAGTA CAAGCTGTCG 
CTGCTGGCCG ATGCCCCGTT CGATGCGAGC AGCTCCAACG ATTGCCTGTA CAAATTCACG 
CTGCTCAATC GCCTCTCGAC CATCGACGAC AAGTTGCAGC TGAAGACGGA GCAGGAGCCG 
ACGACGGACC ACACGGCTCT CTATCTGGAG CCGAACTTTG AGGAGAAGCT GGTCGCGTAC 
TGCCACAGGC TGACGAAGCA TGCCTGGAGC ATGCCCAGCA CAGTGGCCAG CTGGAGCGTG 
GGCCTGCGGG GCATGACGCT GCTGCATCTG GCCGCCGCCT TGGGCTATGC CAAGCTGGTA 
GGCGCCATGC TCAATTGGCG GGCGGAGAAT CCACATATCA TACTCGAAAC GGAGCTGGAC 
GCCCTCAGTC AGGATGTGTA CGGTTTCACG CCGCTCGCCT GGTCCTGTGT GCGCGGCCAT 
GTGGAGTGTT CCCTGCTGCT CTACAAGTGG AATCACAATG CGCTGAAGAT CAAGACGCAG 
AGCCAGCAAA CGGCCCTGGA TTTGGCCAAC CTGAAGGGGC ACAAACATCT CCTGTCCCAG 
ATATTTCGAC TGGAGAAGGA GCGCTGCCGC AAGCCGCACT TGCGAGGCGG CCTGGCCAAT 
CTTTCAATGA ACCTAGGCGT TGAAACGGAA GCGGAGGAGT CGCCGCCGCA GACCTACAAC 
AGCTTTGACC TGGAGATACT CCAAAGAAAG CACGATGGCG TCTTCCTCAG ACCTGGGGCA 
GTGCATAGCA ATCAAAGTCC TCCCAACAAT GGCAGCCGGT ACTCCAAGCG CTCTTCCATA 
GACAGCGGCA TCAACATGGA CATACGCAGC AAGTCAGGCA AGTCCCTGGC CAGGCTGCAC 
AGCAACTTTG AGGGGCACGA CAGCTATGCA TTGGGAGTGG ACTCGCCGCT GGATACGCTG 
ACTGGGACCA ATTGCCTGCT CTCACCGCTA CGTAAAATGG ATTTCGCCCT GTGCGAGGTA 
TCCACGGGCG AGTCGAGCCC CATACACGAC AAAGACATCG ATGGCGATGA CACCTCGACG 
AGCGCCACCG ATGTGACCAT AGGCAATGAC CTGGCCCTGG CCCTGGCCGA TGCAGTTGTA 
GGGGACTCGG ATGCCAAAGT GCTGACTCTG GCCGAACACA TTATAGCAGC AATGCCAGAG 
CGCATCAAAA ATGAAGCCGA TGAAATGATG GTGCTGGGCA GCCCCTTGAC TGAGCCACTG 
ACCTCGGAGT CCTCGGCCCT GACAGACAGC TTTATGGATC CCCTGCTGGA CTCGCTGCCC 
AATACGCATT TCGACAGCGA CTTCAACTTC GACTTCCACG ACCACAGCTA TCGGTACCAC 
GATGTGAGCA CTCCCTGCTC CAGCCTGAGT CCCGCCAGCT CGGGGCCGCT GCAGTCGCCG 
GCCAGCTACT CCATACTGGG CACAGATCCG TCTGTCAGCT CGCCCAGTCC GCCGCCGTCG 
ACCAAGCAGC TGACCGAGTT CCTGCACGCA TCCAGCCTGT CGCAGTATCC GTTCGAGGCG 
GACTTCTCCA AGCTGACGCT GACCGACACG GAGCAGCGGG AGCTGTACGA GGCGGCCAAG 
TGCATACAGA AGGCCTACCG CTCGTACAAG GGCCGGCAGA AGCTCGAGGA GCAGAACAAA 
GAGCGCACTG CCGCCATTGT CATCCAGAAC TATTACAGGC GGTACAAGCA GTACGCCTAT 
TACAGGCAGA TGACCAACGC CGCCCTGGTC ATCCAGCATG GCTATCGTTC CTATCGAAAG 
AACAAACGCT TCAAGAAGAG CGGCCTGGGC CAGGGCATGG CAGGGGGCAG CGATCACGGC 
AGCGTCAGCA GCAACTCTCA GTGCCTGTCC AGCTTCTACG ACCACTACAA GCAGGATCAG 
CAGCAGCTGC AGCAGCAGCA TGAGATGGGC TCGCAGCCGA GCACGCCCAA GGAAACCAGT 
CCCTCGGGTC CCTTGAAACG AACCTACTCG CAGTCCACCC AGAATCAAGC TGCCAGGAAG 
ATCCAACAGT TCATGAGACA ATCACGCATC AAACTACAGA GAGAGCGAGC CGAAAAAGAG 
AAGCTGGTGC ACCAACGCAG GGCGGAATAC CTTCAAAACT TGCAGTATCA AGGGCAGCAG 
GAAATCTTGG CTTGCCTAGA AAATAATTCT GCCCAGAGCA GTGGCAGCAA CACCTATACG 
AACAGCATCA ACAATCTACA ATCCAATCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA30460 ( XM_015184950.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015040436.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant E (Dpse\GA30460), mRNA.

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CloneID	ODe23357
Clone ID Related Accession (Same CDS sequence)	XM_015184950.1 , XM_015184951.1
Accession Version	XM_015184950.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform E
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAATG TGTCCGATGC CAGCTGCAGC GCCTCTTCCC TGAAACTCCA ACGGCAGCAA 
ACTCAAATCA AAGCCAAAAA TGTCCTAATC AGATCCCAGA GCAGCGGCAA GCTTAGCAGA 
AATGAGGCTG AATCCACAAC AACAGCAACA ACAACAGCAA CAGCGAAAAC CACAAAAGCC 
ACAACGAGGA CTCTCAAGAT CGGGGCCATG CAACAGAAGC AGCTACAGCC ACCAAATGGA 
GTCAACGCTA GCAACATCAT CCTGCTGCGG GGCACACGCA ACGAGAACGG CCAGATCATT 
ATCCAGAACA AGCAGGACAT CCTCACCCTG CTCAACGAGC AGCAGCAGCA CCAGGAGAAG 
GCAGCCCAGG CGGCCCAGGC GTCCACAGCC ATCACACTCA ATCAACTGCC AACGACCACC 
ACAGTGGCTC GCAAGACGAT TGTCCAGAGC TCGGGGAGCA GCACCACCAG CACCAGCTCC 
ACCATATCCA TCGTGGCCAG CAGCAGCAGC GGCAGCAGTA GCGGTGTTGG TGCCAAGGAT 
GCCGCTAGCA ACACGATCCT CCTGCAGACG CCCATCAATG CCAGCCAGCT GGAGAGCGTG 
CTGAAGGCCC AGGAGCGAAC GAAACAGGCG AACGGCACGG CACAGACCAA GCTCATAGAG 
AGGCCCTTCC TGCTGAAGCA CGCCACGCGG AGCCTCAGCT CGGAGAGTAA TTGTGACAGC 
AAATCGCCGT TCGTGCTGCA GACACTCAAG CGACTGGAGA AATCGCAATC CATTCTGGTC 
ATACGCAACT CCACATCAGG CAGCACTTCC TCAATGGCCG TTTCTCCGCC TGTGAATGTT 
TCGGCCTCCG TATCATCGAA TGTTTTAAGC GTGACACGCA CTTCAAAGGC GGCCAAGGGC 
ATGGCAGGTG CGCTGCACAA GCTCAAGGCG GCCGCAACGG CAGCAGCCGC AGCGGGAGCA 
GGAGCAGGGG CAGCACCATC CGCATCATCG ACAGGCACAA CCATACTGAG GCTAGGCAAA 
AGCGCCACCA CATTGGCCAT TAACGGAGCT GGGGGCAGCA GCAAACTGGA GGTCGCCGCC 
GCCGCCGCAA CAGCAACCAC AACGTCAACA GCAAATAAAT TGTCCAACAC GGTGACATCG 
GAGCAGCAGC AACAGCAACA ATCGACAACA ACGCAAACGA ATGTGCCACT TGGGAACGAT 
GGCGAACCTA TCAAACTACC CGACAATTTG GAGAGCCTGC CAAGAGCTGA CAGTTTTCCA 
TCACAGCGTC ATCGTTGGAA TACAAATGAG GAAATTGCTG CGATTCTTAT CAGCTTTGAC 
AAGCACTGCG AGTGGCAGTC CAAGGAGGTC CGAACCAGAC CCAAGAGCGG ATCCCTGCTG 
CTGTACTCGC GGAAGAAGGT GCGCTATCGC CGCGACGGCT ATTGCTGGAA GAAGCGCAAG 
GATGGCAAGA CGACGCGCGA GGATCACATG AAACTGAAAG TACAAGGCAC GGAGTGCATC 
TACGGCTGTT ATGTACACTC GGCCATATTG CCCACGTTCC ATCGCAGATG TTACTGGCTG 
TTGCAGAATC CGGACATTGT TTTGGTGCAC TATCTGAATG TGCCGTATCC GGATGATAAT 
AAAATGGCAG TGATTGCCCC CAGCATCACC CTGTGGGGCG ACAAGAAAGA GTGGACCAAG 
GAGGAGCTGG TCAGCCAGCT GAAGCCGATG CTCTCCACAG TCAACTCCGA TGATGACTCC 
GACTCGGGCA ACGACATTGA GATATCGACG GCCGAGACCG TGGAGTCGAT TGTGTGCCAG 
CTGATGGAGA AGCAGCGCTT GTCCCGCCAG GCGGCCCTCG TCAAGCAGCT CGACTGCGGC 
TGCGGGGACT CGGGCTGTGC CGATGGCAAG ACCTGCACGC ATCCCGTGAT GCGGCGCACG 
GGCGGCATCA TCAAGTCCGC GGTGGCCACA TCGGCCTCAG AGAAGCGACT CAACGAGGGG 
GCCTTCAACG GCGGCACGCC CAATGTGGTG GTGGCCAGCA AGCTATACTC CCGCTGGGCG 
GAGCGGCCGC GCCAGCGCAA CGATGGCATG CACCTGGACA ACCCGCACGC ACAGTTCCAC 
AATGCCACGC AACAGCTGCC CCAGGAATCG GCCATCAAGT TTCAGATCAT TCCTCCTCAA 
CAGCAGCAGC AGCAGCAACA ACAGACGGGA CATGGCGATG GCAGCTATCA GCAGCAATAT 
CGTGGTGCCA ATCAGATGCT GGCCGCAAGA AGCAATCTGG TGATGCAGCA GCAACAGCAA 
CAGCAGCAGC AATATCACCA GCAACAGCAG CAACACTTGA GTCTGCAACG ATTCGTGGCC 
AGTCAGAGCC AGAATTCGGC TCATTTGAAT CATCATCATC ATCGCCTGCA AATTGCCAAT 
ACAACAATCA CGGCCACAGC CAGAGATCCT TCCACAACAG TAGCAGCAGC AGCAACAGCA 
GCATCGGCAT CAGCAGCAGG AGGAGGCTCC GTCTTAAATG GAATGGATAC GGAACTAATT 
GAAATCCAAA GCCACATGAC TGCAAACAAA ATCACTAGCT CCGTCAGCAG CAATGGAAAT 
GGCAGCGGCA GCAATCCCAG TGCCAGTGCC AAGCAATTAG CAGCTCAAAC GAACAACGAA 
TTAAATGTCG TAAATAATAA CGACCCCAGC AACAATAACT TTATGTACAA TCAACAACAG 
CAGCAACTGA ATACTTCCAG CAACAGCAAC AACACACAGC AGCAGCAGCA ACATTATTAT 
AAATTGCAAC AGACATCAGC AACCCCAAAC AGTGGCCAAC TCCAACTCCA AAATCAAACC 
CAAACACAAA CACAAACCCC AACCCTTGTC CAACCCCCTG TTGAGGCCAT GTGTATGTCA 
CCTGAGCATC GCAGCGGCAG CAGCAGCAGC AGCAACAACA ATACGGCCTC AACGTCTTCC 
GCTGTCAACA GTCATAACAC CTCCCCCTCC ACAGCCATAG CCACAACCAC GTCCATGGCC 
GCATCTATAT CTATATCCAC ATCTACGTCC GGCACATCGT CCACCTCGAG TTCCTTACAA 
TCGATTATCG ATGGCAATCA GCAGCAACAA ATTACAACGA CATCGACGGC AGCAACTTGT 
GATAATTTAA TGAGCGCCAA TGCGCTCACT GAGCTGAATG GAGGGAGCAG CAGCAGCAGC 
AACAATCAGG CCACAGATGG CCACAACAAG AGTCAATCCC AATCGCAGTC TCAGCCGCAA 
ACTCTGAGCC AAAATGGTTG TGCAACATTC AGTGCTTCCA GTGATAATAG CAGCCAAATT 
AGCGCCAGCG ACGAGAGTAG CAGCTTTGGG GCCAACAATC AGAACAACAG CATACACAGC 
ATCCACAGCA GCCACAGCAG CAGCAGTAAC AGCAGCGAGG ACGAGCCCCA GGCGGGCAAT 
GCTGCCGAGA CCCTGAGCTT CTTCAACGAA ACCCTGGATC TATCCCATGA GGATATTCAA 
CGTACACTGA TAGCCAATAT GCAGCCGTAC AATACGGCTG CAGGCGAAGC AGCAGCGACA 
ACGATAGAAC CTTTGGCAAC AAACGGCAGC CACAGTAGCA GCAACAGCAA TGGCAATCCT 
CAAGAAGTGG AGAAGAAACC TTCAGTCGGC CAGGAAGTCG AAGCCGAACC TGAGGAGGAT 
GACACAGACG ATGTGTTTGC CAACCTGGAT GCCTTCGATA TGCTCGTGGA GTTCCCCGAG 
CTGGATCTGG ATGATAAGCA GGCCCTGAAC AACACGGCCT TGGAGCAGGG CTCCTATCTG 
GGACAGGCGG CCGCCCAAAC GCAGCAGCCC CGCAAGATCC ACAACATCTG CGACTTCAGT 
CCGGAGTGGT CGTACACCGA GGGCGGAGTC AAGGTACTTG TGGCCGGACC CTGGACGAGC 
AGCAATGGTG CTGGTGCCTA CACGGTGCTC TTCGATGCCC AGCCCGTGCC CACGCAGATG 
GTGCAGGAGG GAGTGCTCCG TTGCTACTGT CCTGCCCACG AGGCGGGCTT TGTGACGCTC 
CAGGTGGCCT GCGGCGGCTT TCTCGTGTCC AATTCGGTCA TGTTCGAGTA CAAGCTGTCG 
CTGCTGGCCG ATGCCCCGTT CGATGCGAGC AGCTCCAACG ATTGCCTGTA CAAATTCACG 
CTGCTCAATC GCCTCTCGAC CATCGACGAC AAGTTGCAGC TGAAGACGGA GCAGGAGCCG 
ACGACGGACC ACACGGCTCT CTATCTGGAG CCGAACTTTG AGGAGAAGCT GGTCGCGTAC 
TGCCACAGGC TGACGAAGCA TGCCTGGAGC ATGCCCAGCA CAGTGGCCAG CTGGAGCGTG 
GGCCTGCGGG GCATGACGCT GCTGCATCTG GCCGCCGCCT TGGGCTATGC CAAGCTGGTA 
GGCGCCATGC TCAATTGGCG GGCGGAGAAT CCACATATCA TACTCGAAAC GGAGCTGGAC 
GCCCTCAGTC AGGATGTGTA CGGTTTCACG CCGCTCGCCT GGTCCTGTGT GCGCGGCCAT 
GTGGAGTGTT CCCTGCTGCT CTACAAGTGG AATCACAATG CGCTGAAGAT CAAGACGCAG 
AGCCAGCAAA CGGCCCTGGA TTTGGCCAAC CTGAAGGGGC ACAAACATCT CCTGTCCCAG 
ATATTTCGAC TGGAGAAGGA GCGCTGCCGC AAGCCGCACT TGCGAGGCGG CCTGGCCAAT 
CTTTCAATGA ACCTAGGCGT TGAAACGGAA GCGGAGGAGT CGCCGCCGCA GACCTACAAC 
AGCTTTGACC TGGAGATACT CCAAAGAAAG CACGATGGCG TCTTCCTCAG ACCTGGGGCA 
GTGCATAGCA ATCAAAGTCC TCCCAACAAT GGCAGCCGGT ACTCCAAGCG CTCTTCCATA 
GACAGCGGCA TCAACATGGA CATACGCAGC AAGTCAGGCA AGTCCCTGGC CAGGCTGCAC 
AGCAACTTTG AGGGGCACGA CAGCTATGCA TTGGGAGTGG ACTCGCCGCT GGATACGCTG 
ACTGGGACCA ATTGCCTGCT CTCACCGCTA CGTAAAATGG ATTTCGCCCT GTGCGAGGTA 
TCCACGGGCG AGTCGAGCCC CATACACGAC AAAGACATCG ATGGCGATGA CACCTCGACG 
AGCGCCACCG ATGTGACCAT AGGCAATGAC CTGGCCCTGG CCCTGGCCGA TGCAGTTGTA 
GGGGACTCGG ATGCCAAAGT GCTGACTCTG GCCGAACACA TTATAGCAGC AATGCCAGAG 
CGCATCAAAA ATGAAGCCGA TGAAATGATG GTGCTGGGCA GCCCCTTGAC TGAGCCACTG 
ACCTCGGAGT CCTCGGCCCT GACAGACAGC TTTATGGATC CCCTGCTGGA CTCGCTGCCC 
AATACGCATT TCGACAGCGA CTTCAACTTC GACTTCCACG ACCACAGCTA TCGGTACCAC 
GATGTGAGCA CTCCCTGCTC CAGCCTGAGT CCCGCCAGCT CGGGGCCGCT GCAGTCGCCG 
GCCAGCTACT CCATACTGGG CACAGATCCG TCTGTCAGCT CGCCCAGTCC GCCGCCGTCG 
ACCAAGCAGC TGACCGAGTT CCTGCACGCA TCCAGCCTGT CGCAGTATCC GTTCGAGGCG 
GACTTCTCCA AGCTGACGCT GACCGACACG GAGCAGCGGG AGCTGTACGA GGCGGCCAAG 
TGCATACAGA AGGCCTACCG CTCGTACAAG GGCCGGCAGA AGCTCGAGGA GCAGAACAAA 
GAGCGCACTG CCGCCATTGT CATCCAGAAC TATTACAGGC GGTACAAGCA GTACGCCTAT 
TACAGGCAGA TGACCAACGC CGCCCTGGTC ATCCAGCATG GCTATCGTTC CTATCGAAAG 
AACAAACGCT TCAAGAAGAG CGGCCTGGGC CAGGGCATGG CAGGGGGCAG CGATCACGGC 
AGCGTCAGCA GCAACTCTCA GTGCCTGTCC AGCTTCTACG ACCACTACAA GCAGGATCAG 
CAGCAGCTGC AGCAGCAGCA TGAGATGGGC TCGCAGCCGA GCACGCCCAA GGAAACCAGT 
CCCTCGGGTC CCTTGAAACG AACCTACTCG CAGTCCACCC AGAATCAAGC TGCCAGGAAG 
ATCCAACAGT TCATGAGACA ATCACGCATC AACATTTGGG ATGCCAATTT GACGACTTTG 
ACTACAGAGA GAGCGAGCCG AAAAAGAGAA GCTGGTGCAC CAACGCAGGG CGGAATACCT 
TCAAAACTTG CAGTATCAAG GGCAGCAGGA AATCTTGGCT TGCCTAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015040436.1
CDS	372..6605
Translation	MENVSDASCSASSLKLQRQQTQIKAKNVLIRSQSSGKLSRNEAESTTTATTTATAKTTKATTRTLKIGAMQQKQLQPPNGVNASNIILLRGTRNENGQIIIQNKQDILTLLNEQQQHQEKAAQAAQASTAITLNQLPTTTTVARKTIVQSSGSSTTSTSSTISIVASSSSGSSSGVGAKDAASNTILLQTPINASQLESVLKAQERTKQANGTAQTKLIERPFLLKHATRSLSSESNCDSKSPFVLQTLKRLEKSQSILVIRNSTSGSTSSMAVSPPVNVSASVSSNVLSVTRTSKAAKGMAGALHKLKAAATAAAAAGAGAGAAPSASSTGTTILRLGKSATTLAINGAGGSSKLEVAAAAATATTTSTANKLSNTVTSEQQQQQQSTTTQTNVPLGNDGEPIKLPDNLESLPRADSFPSQRHRWNTNEEIAAILISFDKHCEWQSKEVRTRPKSGSLLLYSRKKVRYRRDGYCWKKRKDGKTTREDHMKLKVQGTECIYGCYVHSAILPTFHRRCYWLLQNPDIVLVHYLNVPYPDDNKMAVIAPSITLWGDKKEWTKEELVSQLKPMLSTVNSDDDSDSGNDIEISTAETVESIVCQLMEKQRLSRQAALVKQLDCGCGDSGCADGKTCTHPVMRRTGGIIKSAVATSASEKRLNEGAFNGGTPNVVVASKLYSRWAERPRQRNDGMHLDNPHAQFHNATQQLPQESAIKFQIIPPQQQQQQQQQTGHGDGSYQQQYRGANQMLAARSNLVMQQQQQQQQQYHQQQQQHLSLQRFVASQSQNSAHLNHHHHRLQIANTTITATARDPSTTVAAAATAASASAAGGGSVLNGMDTELIEIQSHMTANKITSSVSSNGNGSGSNPSASAKQLAAQTNNELNVVNNNDPSNNNFMYNQQQQQLNTSSNSNNTQQQQQHYYKLQQTSATPNSGQLQLQNQTQTQTQTPTLVQPPVEAMCMSPEHRSGSSSSSNNNTASTSSAVNSHNTSPSTAIATTTSMAASISISTSTSGTSSTSSSLQSIIDGNQQQQITTTSTAATCDNLMSANALTELNGGSSSSSNNQATDGHNKSQSQSQSQPQTLSQNGCATFSASSDNSSQISASDESSSFGANNQNNSIHSIHSSHSSSSNSSEDEPQAGNAAETLSFFNETLDLSHEDIQRTLIANMQPYNTAAGEAAATTIEPLATNGSHSSSNSNGNPQEVEKKPSVGQEVEAEPEEDDTDDVFANLDAFDMLVEFPELDLDDKQALNNTALEQGSYLGQAAAQTQQPRKIHNICDFSPEWSYTEGGVKVLVAGPWTSSNGAGAYTVLFDAQPVPTQMVQEGVLRCYCPAHEAGFVTLQVACGGFLVSNSVMFEYKLSLLADAPFDASSSNDCLYKFTLLNRLSTIDDKLQLKTEQEPTTDHTALYLEPNFEEKLVAYCHRLTKHAWSMPSTVASWSVGLRGMTLLHLAAALGYAKLVGAMLNWRAENPHIILETELDALSQDVYGFTPLAWSCVRGHVECSLLLYKWNHNALKIKTQSQQTALDLANLKGHKHLLSQIFRLEKERCRKPHLRGGLANLSMNLGVETEAEESPPQTYNSFDLEILQRKHDGVFLRPGAVHSNQSPPNNGSRYSKRSSIDSGINMDIRSKSGKSLARLHSNFEGHDSYALGVDSPLDTLTGTNCLLSPLRKMDFALCEVSTGESSPIHDKDIDGDDTSTSATDVTIGNDLALALADAVVGDSDAKVLTLAEHIIAAMPERIKNEADEMMVLGSPLTEPLTSESSALTDSFMDPLLDSLPNTHFDSDFNFDFHDHSYRYHDVSTPCSSLSPASSGPLQSPASYSILGTDPSVSSPSPPPSTKQLTEFLHASSLSQYPFEADFSKLTLTDTEQRELYEAAKCIQKAYRSYKGRQKLEEQNKERTAAIVIQNYYRRYKQYAYYRQMTNAALVIQHGYRSYRKNKRFKKSGLGQGMAGGSDHGSVSSNSQCLSSFYDHYKQDQQQLQQQHEMGSQPSTPKETSPSGPLKRTYSQSTQNQAARKIQQFMRQSRINIWDANLTTLTTERASRKREAGAPTQGGIPSKLAVSRAAGNLGLPRK

Target ORF information:

RefSeq Version	XM_015184950.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant E (Dpse\GA30460), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015184950.1

ORF Insert Sequence:

ATGGAAAATG TGTCCGATGC CAGCTGCAGC GCCTCTTCCC TGAAACTCCA ACGGCAGCAA 
ACTCAAATCA AAGCCAAAAA TGTCCTAATC AGATCCCAGA GCAGCGGCAA GCTTAGCAGA 
AATGAGGCTG AATCCACAAC AACAGCAACA ACAACAGCAA CAGCGAAAAC CACAAAAGCC 
ACAACGAGGA CTCTCAAGAT CGGGGCCATG CAACAGAAGC AGCTACAGCC ACCAAATGGA 
GTCAACGCTA GCAACATCAT CCTGCTGCGG GGCACACGCA ACGAGAACGG CCAGATCATT 
ATCCAGAACA AGCAGGACAT CCTCACCCTG CTCAACGAGC AGCAGCAGCA CCAGGAGAAG 
GCAGCCCAGG CGGCCCAGGC GTCCACAGCC ATCACACTCA ATCAACTGCC AACGACCACC 
ACAGTGGCTC GCAAGACGAT TGTCCAGAGC TCGGGGAGCA GCACCACCAG CACCAGCTCC 
ACCATATCCA TCGTGGCCAG CAGCAGCAGC GGCAGCAGTA GCGGTGTTGG TGCCAAGGAT 
GCCGCTAGCA ACACGATCCT CCTGCAGACG CCCATCAATG CCAGCCAGCT GGAGAGCGTG 
CTGAAGGCCC AGGAGCGAAC GAAACAGGCG AACGGCACGG CACAGACCAA GCTCATAGAG 
AGGCCCTTCC TGCTGAAGCA CGCCACGCGG AGCCTCAGCT CGGAGAGTAA TTGTGACAGC 
AAATCGCCGT TCGTGCTGCA GACACTCAAG CGACTGGAGA AATCGCAATC CATTCTGGTC 
ATACGCAACT CCACATCAGG CAGCACTTCC TCAATGGCCG TTTCTCCGCC TGTGAATGTT 
TCGGCCTCCG TATCATCGAA TGTTTTAAGC GTGACACGCA CTTCAAAGGC GGCCAAGGGC 
ATGGCAGGTG CGCTGCACAA GCTCAAGGCG GCCGCAACGG CAGCAGCCGC AGCGGGAGCA 
GGAGCAGGGG CAGCACCATC CGCATCATCG ACAGGCACAA CCATACTGAG GCTAGGCAAA 
AGCGCCACCA CATTGGCCAT TAACGGAGCT GGGGGCAGCA GCAAACTGGA GGTCGCCGCC 
GCCGCCGCAA CAGCAACCAC AACGTCAACA GCAAATAAAT TGTCCAACAC GGTGACATCG 
GAGCAGCAGC AACAGCAACA ATCGACAACA ACGCAAACGA ATGTGCCACT TGGGAACGAT 
GGCGAACCTA TCAAACTACC CGACAATTTG GAGAGCCTGC CAAGAGCTGA CAGTTTTCCA 
TCACAGCGTC ATCGTTGGAA TACAAATGAG GAAATTGCTG CGATTCTTAT CAGCTTTGAC 
AAGCACTGCG AGTGGCAGTC CAAGGAGGTC CGAACCAGAC CCAAGAGCGG ATCCCTGCTG 
CTGTACTCGC GGAAGAAGGT GCGCTATCGC CGCGACGGCT ATTGCTGGAA GAAGCGCAAG 
GATGGCAAGA CGACGCGCGA GGATCACATG AAACTGAAAG TACAAGGCAC GGAGTGCATC 
TACGGCTGTT ATGTACACTC GGCCATATTG CCCACGTTCC ATCGCAGATG TTACTGGCTG 
TTGCAGAATC CGGACATTGT TTTGGTGCAC TATCTGAATG TGCCGTATCC GGATGATAAT 
AAAATGGCAG TGATTGCCCC CAGCATCACC CTGTGGGGCG ACAAGAAAGA GTGGACCAAG 
GAGGAGCTGG TCAGCCAGCT GAAGCCGATG CTCTCCACAG TCAACTCCGA TGATGACTCC 
GACTCGGGCA ACGACATTGA GATATCGACG GCCGAGACCG TGGAGTCGAT TGTGTGCCAG 
CTGATGGAGA AGCAGCGCTT GTCCCGCCAG GCGGCCCTCG TCAAGCAGCT CGACTGCGGC 
TGCGGGGACT CGGGCTGTGC CGATGGCAAG ACCTGCACGC ATCCCGTGAT GCGGCGCACG 
GGCGGCATCA TCAAGTCCGC GGTGGCCACA TCGGCCTCAG AGAAGCGACT CAACGAGGGG 
GCCTTCAACG GCGGCACGCC CAATGTGGTG GTGGCCAGCA AGCTATACTC CCGCTGGGCG 
GAGCGGCCGC GCCAGCGCAA CGATGGCATG CACCTGGACA ACCCGCACGC ACAGTTCCAC 
AATGCCACGC AACAGCTGCC CCAGGAATCG GCCATCAAGT TTCAGATCAT TCCTCCTCAA 
CAGCAGCAGC AGCAGCAACA ACAGACGGGA CATGGCGATG GCAGCTATCA GCAGCAATAT 
CGTGGTGCCA ATCAGATGCT GGCCGCAAGA AGCAATCTGG TGATGCAGCA GCAACAGCAA 
CAGCAGCAGC AATATCACCA GCAACAGCAG CAACACTTGA GTCTGCAACG ATTCGTGGCC 
AGTCAGAGCC AGAATTCGGC TCATTTGAAT CATCATCATC ATCGCCTGCA AATTGCCAAT 
ACAACAATCA CGGCCACAGC CAGAGATCCT TCCACAACAG TAGCAGCAGC AGCAACAGCA 
GCATCGGCAT CAGCAGCAGG AGGAGGCTCC GTCTTAAATG GAATGGATAC GGAACTAATT 
GAAATCCAAA GCCACATGAC TGCAAACAAA ATCACTAGCT CCGTCAGCAG CAATGGAAAT 
GGCAGCGGCA GCAATCCCAG TGCCAGTGCC AAGCAATTAG CAGCTCAAAC GAACAACGAA 
TTAAATGTCG TAAATAATAA CGACCCCAGC AACAATAACT TTATGTACAA TCAACAACAG 
CAGCAACTGA ATACTTCCAG CAACAGCAAC AACACACAGC AGCAGCAGCA ACATTATTAT 
AAATTGCAAC AGACATCAGC AACCCCAAAC AGTGGCCAAC TCCAACTCCA AAATCAAACC 
CAAACACAAA CACAAACCCC AACCCTTGTC CAACCCCCTG TTGAGGCCAT GTGTATGTCA 
CCTGAGCATC GCAGCGGCAG CAGCAGCAGC AGCAACAACA ATACGGCCTC AACGTCTTCC 
GCTGTCAACA GTCATAACAC CTCCCCCTCC ACAGCCATAG CCACAACCAC GTCCATGGCC 
GCATCTATAT CTATATCCAC ATCTACGTCC GGCACATCGT CCACCTCGAG TTCCTTACAA 
TCGATTATCG ATGGCAATCA GCAGCAACAA ATTACAACGA CATCGACGGC AGCAACTTGT 
GATAATTTAA TGAGCGCCAA TGCGCTCACT GAGCTGAATG GAGGGAGCAG CAGCAGCAGC 
AACAATCAGG CCACAGATGG CCACAACAAG AGTCAATCCC AATCGCAGTC TCAGCCGCAA 
ACTCTGAGCC AAAATGGTTG TGCAACATTC AGTGCTTCCA GTGATAATAG CAGCCAAATT 
AGCGCCAGCG ACGAGAGTAG CAGCTTTGGG GCCAACAATC AGAACAACAG CATACACAGC 
ATCCACAGCA GCCACAGCAG CAGCAGTAAC AGCAGCGAGG ACGAGCCCCA GGCGGGCAAT 
GCTGCCGAGA CCCTGAGCTT CTTCAACGAA ACCCTGGATC TATCCCATGA GGATATTCAA 
CGTACACTGA TAGCCAATAT GCAGCCGTAC AATACGGCTG CAGGCGAAGC AGCAGCGACA 
ACGATAGAAC CTTTGGCAAC AAACGGCAGC CACAGTAGCA GCAACAGCAA TGGCAATCCT 
CAAGAAGTGG AGAAGAAACC TTCAGTCGGC CAGGAAGTCG AAGCCGAACC TGAGGAGGAT 
GACACAGACG ATGTGTTTGC CAACCTGGAT GCCTTCGATA TGCTCGTGGA GTTCCCCGAG 
CTGGATCTGG ATGATAAGCA GGCCCTGAAC AACACGGCCT TGGAGCAGGG CTCCTATCTG 
GGACAGGCGG CCGCCCAAAC GCAGCAGCCC CGCAAGATCC ACAACATCTG CGACTTCAGT 
CCGGAGTGGT CGTACACCGA GGGCGGAGTC AAGGTACTTG TGGCCGGACC CTGGACGAGC 
AGCAATGGTG CTGGTGCCTA CACGGTGCTC TTCGATGCCC AGCCCGTGCC CACGCAGATG 
GTGCAGGAGG GAGTGCTCCG TTGCTACTGT CCTGCCCACG AGGCGGGCTT TGTGACGCTC 
CAGGTGGCCT GCGGCGGCTT TCTCGTGTCC AATTCGGTCA TGTTCGAGTA CAAGCTGTCG 
CTGCTGGCCG ATGCCCCGTT CGATGCGAGC AGCTCCAACG ATTGCCTGTA CAAATTCACG 
CTGCTCAATC GCCTCTCGAC CATCGACGAC AAGTTGCAGC TGAAGACGGA GCAGGAGCCG 
ACGACGGACC ACACGGCTCT CTATCTGGAG CCGAACTTTG AGGAGAAGCT GGTCGCGTAC 
TGCCACAGGC TGACGAAGCA TGCCTGGAGC ATGCCCAGCA CAGTGGCCAG CTGGAGCGTG 
GGCCTGCGGG GCATGACGCT GCTGCATCTG GCCGCCGCCT TGGGCTATGC CAAGCTGGTA 
GGCGCCATGC TCAATTGGCG GGCGGAGAAT CCACATATCA TACTCGAAAC GGAGCTGGAC 
GCCCTCAGTC AGGATGTGTA CGGTTTCACG CCGCTCGCCT GGTCCTGTGT GCGCGGCCAT 
GTGGAGTGTT CCCTGCTGCT CTACAAGTGG AATCACAATG CGCTGAAGAT CAAGACGCAG 
AGCCAGCAAA CGGCCCTGGA TTTGGCCAAC CTGAAGGGGC ACAAACATCT CCTGTCCCAG 
ATATTTCGAC TGGAGAAGGA GCGCTGCCGC AAGCCGCACT TGCGAGGCGG CCTGGCCAAT 
CTTTCAATGA ACCTAGGCGT TGAAACGGAA GCGGAGGAGT CGCCGCCGCA GACCTACAAC 
AGCTTTGACC TGGAGATACT CCAAAGAAAG CACGATGGCG TCTTCCTCAG ACCTGGGGCA 
GTGCATAGCA ATCAAAGTCC TCCCAACAAT GGCAGCCGGT ACTCCAAGCG CTCTTCCATA 
GACAGCGGCA TCAACATGGA CATACGCAGC AAGTCAGGCA AGTCCCTGGC CAGGCTGCAC 
AGCAACTTTG AGGGGCACGA CAGCTATGCA TTGGGAGTGG ACTCGCCGCT GGATACGCTG 
ACTGGGACCA ATTGCCTGCT CTCACCGCTA CGTAAAATGG ATTTCGCCCT GTGCGAGGTA 
TCCACGGGCG AGTCGAGCCC CATACACGAC AAAGACATCG ATGGCGATGA CACCTCGACG 
AGCGCCACCG ATGTGACCAT AGGCAATGAC CTGGCCCTGG CCCTGGCCGA TGCAGTTGTA 
GGGGACTCGG ATGCCAAAGT GCTGACTCTG GCCGAACACA TTATAGCAGC AATGCCAGAG 
CGCATCAAAA ATGAAGCCGA TGAAATGATG GTGCTGGGCA GCCCCTTGAC TGAGCCACTG 
ACCTCGGAGT CCTCGGCCCT GACAGACAGC TTTATGGATC CCCTGCTGGA CTCGCTGCCC 
AATACGCATT TCGACAGCGA CTTCAACTTC GACTTCCACG ACCACAGCTA TCGGTACCAC 
GATGTGAGCA CTCCCTGCTC CAGCCTGAGT CCCGCCAGCT CGGGGCCGCT GCAGTCGCCG 
GCCAGCTACT CCATACTGGG CACAGATCCG TCTGTCAGCT CGCCCAGTCC GCCGCCGTCG 
ACCAAGCAGC TGACCGAGTT CCTGCACGCA TCCAGCCTGT CGCAGTATCC GTTCGAGGCG 
GACTTCTCCA AGCTGACGCT GACCGACACG GAGCAGCGGG AGCTGTACGA GGCGGCCAAG 
TGCATACAGA AGGCCTACCG CTCGTACAAG GGCCGGCAGA AGCTCGAGGA GCAGAACAAA 
GAGCGCACTG CCGCCATTGT CATCCAGAAC TATTACAGGC GGTACAAGCA GTACGCCTAT 
TACAGGCAGA TGACCAACGC CGCCCTGGTC ATCCAGCATG GCTATCGTTC CTATCGAAAG 
AACAAACGCT TCAAGAAGAG CGGCCTGGGC CAGGGCATGG CAGGGGGCAG CGATCACGGC 
AGCGTCAGCA GCAACTCTCA GTGCCTGTCC AGCTTCTACG ACCACTACAA GCAGGATCAG 
CAGCAGCTGC AGCAGCAGCA TGAGATGGGC TCGCAGCCGA GCACGCCCAA GGAAACCAGT 
CCCTCGGGTC CCTTGAAACG AACCTACTCG CAGTCCACCC AGAATCAAGC TGCCAGGAAG 
ATCCAACAGT TCATGAGACA ATCACGCATC AACATTTGGG ATGCCAATTT GACGACTTTG 
ACTACAGAGA GAGCGAGCCG AAAAAGAGAA GCTGGTGCAC CAACGCAGGG CGGAATACCT 
TCAAAACTTG CAGTATCAAG GGCAGCAGGA AATCTTGGCT TGCCTAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA30460 ( XM_015184951.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015040437.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant F (Dpse\GA30460), mRNA.

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CloneID	ODe23357
Clone ID Related Accession (Same CDS sequence)	XM_015184950.1 , XM_015184951.1
Accession Version	XM_015184951.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform F
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAATG TGTCCGATGC CAGCTGCAGC GCCTCTTCCC TGAAACTCCA ACGGCAGCAA 
ACTCAAATCA AAGCCAAAAA TGTCCTAATC AGATCCCAGA GCAGCGGCAA GCTTAGCAGA 
AATGAGGCTG AATCCACAAC AACAGCAACA ACAACAGCAA CAGCGAAAAC CACAAAAGCC 
ACAACGAGGA CTCTCAAGAT CGGGGCCATG CAACAGAAGC AGCTACAGCC ACCAAATGGA 
GTCAACGCTA GCAACATCAT CCTGCTGCGG GGCACACGCA ACGAGAACGG CCAGATCATT 
ATCCAGAACA AGCAGGACAT CCTCACCCTG CTCAACGAGC AGCAGCAGCA CCAGGAGAAG 
GCAGCCCAGG CGGCCCAGGC GTCCACAGCC ATCACACTCA ATCAACTGCC AACGACCACC 
ACAGTGGCTC GCAAGACGAT TGTCCAGAGC TCGGGGAGCA GCACCACCAG CACCAGCTCC 
ACCATATCCA TCGTGGCCAG CAGCAGCAGC GGCAGCAGTA GCGGTGTTGG TGCCAAGGAT 
GCCGCTAGCA ACACGATCCT CCTGCAGACG CCCATCAATG CCAGCCAGCT GGAGAGCGTG 
CTGAAGGCCC AGGAGCGAAC GAAACAGGCG AACGGCACGG CACAGACCAA GCTCATAGAG 
AGGCCCTTCC TGCTGAAGCA CGCCACGCGG AGCCTCAGCT CGGAGAGTAA TTGTGACAGC 
AAATCGCCGT TCGTGCTGCA GACACTCAAG CGACTGGAGA AATCGCAATC CATTCTGGTC 
ATACGCAACT CCACATCAGG CAGCACTTCC TCAATGGCCG TTTCTCCGCC TGTGAATGTT 
TCGGCCTCCG TATCATCGAA TGTTTTAAGC GTGACACGCA CTTCAAAGGC GGCCAAGGGC 
ATGGCAGGTG CGCTGCACAA GCTCAAGGCG GCCGCAACGG CAGCAGCCGC AGCGGGAGCA 
GGAGCAGGGG CAGCACCATC CGCATCATCG ACAGGCACAA CCATACTGAG GCTAGGCAAA 
AGCGCCACCA CATTGGCCAT TAACGGAGCT GGGGGCAGCA GCAAACTGGA GGTCGCCGCC 
GCCGCCGCAA CAGCAACCAC AACGTCAACA GCAAATAAAT TGTCCAACAC GGTGACATCG 
GAGCAGCAGC AACAGCAACA ATCGACAACA ACGCAAACGA ATGTGCCACT TGGGAACGAT 
GGCGAACCTA TCAAACTACC CGACAATTTG GAGAGCCTGC CAAGAGCTGA CAGTTTTCCA 
TCACAGCGTC ATCGTTGGAA TACAAATGAG GAAATTGCTG CGATTCTTAT CAGCTTTGAC 
AAGCACTGCG AGTGGCAGTC CAAGGAGGTC CGAACCAGAC CCAAGAGCGG ATCCCTGCTG 
CTGTACTCGC GGAAGAAGGT GCGCTATCGC CGCGACGGCT ATTGCTGGAA GAAGCGCAAG 
GATGGCAAGA CGACGCGCGA GGATCACATG AAACTGAAAG TACAAGGCAC GGAGTGCATC 
TACGGCTGTT ATGTACACTC GGCCATATTG CCCACGTTCC ATCGCAGATG TTACTGGCTG 
TTGCAGAATC CGGACATTGT TTTGGTGCAC TATCTGAATG TGCCGTATCC GGATGATAAT 
AAAATGGCAG TGATTGCCCC CAGCATCACC CTGTGGGGCG ACAAGAAAGA GTGGACCAAG 
GAGGAGCTGG TCAGCCAGCT GAAGCCGATG CTCTCCACAG TCAACTCCGA TGATGACTCC 
GACTCGGGCA ACGACATTGA GATATCGACG GCCGAGACCG TGGAGTCGAT TGTGTGCCAG 
CTGATGGAGA AGCAGCGCTT GTCCCGCCAG GCGGCCCTCG TCAAGCAGCT CGACTGCGGC 
TGCGGGGACT CGGGCTGTGC CGATGGCAAG ACCTGCACGC ATCCCGTGAT GCGGCGCACG 
GGCGGCATCA TCAAGTCCGC GGTGGCCACA TCGGCCTCAG AGAAGCGACT CAACGAGGGG 
GCCTTCAACG GCGGCACGCC CAATGTGGTG GTGGCCAGCA AGCTATACTC CCGCTGGGCG 
GAGCGGCCGC GCCAGCGCAA CGATGGCATG CACCTGGACA ACCCGCACGC ACAGTTCCAC 
AATGCCACGC AACAGCTGCC CCAGGAATCG GCCATCAAGT TTCAGATCAT TCCTCCTCAA 
CAGCAGCAGC AGCAGCAACA ACAGACGGGA CATGGCGATG GCAGCTATCA GCAGCAATAT 
CGTGGTGCCA ATCAGATGCT GGCCGCAAGA AGCAATCTGG TGATGCAGCA GCAACAGCAA 
CAGCAGCAGC AATATCACCA GCAACAGCAG CAACACTTGA GTCTGCAACG ATTCGTGGCC 
AGTCAGAGCC AGAATTCGGC TCATTTGAAT CATCATCATC ATCGCCTGCA AATTGCCAAT 
ACAACAATCA CGGCCACAGC CAGAGATCCT TCCACAACAG TAGCAGCAGC AGCAACAGCA 
GCATCGGCAT CAGCAGCAGG AGGAGGCTCC GTCTTAAATG GAATGGATAC GGAACTAATT 
GAAATCCAAA GCCACATGAC TGCAAACAAA ATCACTAGCT CCGTCAGCAG CAATGGAAAT 
GGCAGCGGCA GCAATCCCAG TGCCAGTGCC AAGCAATTAG CAGCTCAAAC GAACAACGAA 
TTAAATGTCG TAAATAATAA CGACCCCAGC AACAATAACT TTATGTACAA TCAACAACAG 
CAGCAACTGA ATACTTCCAG CAACAGCAAC AACACACAGC AGCAGCAGCA ACATTATTAT 
AAATTGCAAC AGACATCAGC AACCCCAAAC AGTGGCCAAC TCCAACTCCA AAATCAAACC 
CAAACACAAA CACAAACCCC AACCCTTGTC CAACCCCCTG TTGAGGCCAT GTGTATGTCA 
CCTGAGCATC GCAGCGGCAG CAGCAGCAGC AGCAACAACA ATACGGCCTC AACGTCTTCC 
GCTGTCAACA GTCATAACAC CTCCCCCTCC ACAGCCATAG CCACAACCAC GTCCATGGCC 
GCATCTATAT CTATATCCAC ATCTACGTCC GGCACATCGT CCACCTCGAG TTCCTTACAA 
TCGATTATCG ATGGCAATCA GCAGCAACAA ATTACAACGA CATCGACGGC AGCAACTTGT 
GATAATTTAA TGAGCGCCAA TGCGCTCACT GAGCTGAATG GAGGGAGCAG CAGCAGCAGC 
AACAATCAGG CCACAGATGG CCACAACAAG AGTCAATCCC AATCGCAGTC TCAGCCGCAA 
ACTCTGAGCC AAAATGGTTG TGCAACATTC AGTGCTTCCA GTGATAATAG CAGCCAAATT 
AGCGCCAGCG ACGAGAGTAG CAGCTTTGGG GCCAACAATC AGAACAACAG CATACACAGC 
ATCCACAGCA GCCACAGCAG CAGCAGTAAC AGCAGCGAGG ACGAGCCCCA GGCGGGCAAT 
GCTGCCGAGA CCCTGAGCTT CTTCAACGAA ACCCTGGATC TATCCCATGA GGATATTCAA 
CGTACACTGA TAGCCAATAT GCAGCCGTAC AATACGGCTG CAGGCGAAGC AGCAGCGACA 
ACGATAGAAC CTTTGGCAAC AAACGGCAGC CACAGTAGCA GCAACAGCAA TGGCAATCCT 
CAAGAAGTGG AGAAGAAACC TTCAGTCGGC CAGGAAGTCG AAGCCGAACC TGAGGAGGAT 
GACACAGACG ATGTGTTTGC CAACCTGGAT GCCTTCGATA TGCTCGTGGA GTTCCCCGAG 
CTGGATCTGG ATGATAAGCA GGCCCTGAAC AACACGGCCT TGGAGCAGGG CTCCTATCTG 
GGACAGGCGG CCGCCCAAAC GCAGCAGCCC CGCAAGATCC ACAACATCTG CGACTTCAGT 
CCGGAGTGGT CGTACACCGA GGGCGGAGTC AAGGTACTTG TGGCCGGACC CTGGACGAGC 
AGCAATGGTG CTGGTGCCTA CACGGTGCTC TTCGATGCCC AGCCCGTGCC CACGCAGATG 
GTGCAGGAGG GAGTGCTCCG TTGCTACTGT CCTGCCCACG AGGCGGGCTT TGTGACGCTC 
CAGGTGGCCT GCGGCGGCTT TCTCGTGTCC AATTCGGTCA TGTTCGAGTA CAAGCTGTCG 
CTGCTGGCCG ATGCCCCGTT CGATGCGAGC AGCTCCAACG ATTGCCTGTA CAAATTCACG 
CTGCTCAATC GCCTCTCGAC CATCGACGAC AAGTTGCAGC TGAAGACGGA GCAGGAGCCG 
ACGACGGACC ACACGGCTCT CTATCTGGAG CCGAACTTTG AGGAGAAGCT GGTCGCGTAC 
TGCCACAGGC TGACGAAGCA TGCCTGGAGC ATGCCCAGCA CAGTGGCCAG CTGGAGCGTG 
GGCCTGCGGG GCATGACGCT GCTGCATCTG GCCGCCGCCT TGGGCTATGC CAAGCTGGTA 
GGCGCCATGC TCAATTGGCG GGCGGAGAAT CCACATATCA TACTCGAAAC GGAGCTGGAC 
GCCCTCAGTC AGGATGTGTA CGGTTTCACG CCGCTCGCCT GGTCCTGTGT GCGCGGCCAT 
GTGGAGTGTT CCCTGCTGCT CTACAAGTGG AATCACAATG CGCTGAAGAT CAAGACGCAG 
AGCCAGCAAA CGGCCCTGGA TTTGGCCAAC CTGAAGGGGC ACAAACATCT CCTGTCCCAG 
ATATTTCGAC TGGAGAAGGA GCGCTGCCGC AAGCCGCACT TGCGAGGCGG CCTGGCCAAT 
CTTTCAATGA ACCTAGGCGT TGAAACGGAA GCGGAGGAGT CGCCGCCGCA GACCTACAAC 
AGCTTTGACC TGGAGATACT CCAAAGAAAG CACGATGGCG TCTTCCTCAG ACCTGGGGCA 
GTGCATAGCA ATCAAAGTCC TCCCAACAAT GGCAGCCGGT ACTCCAAGCG CTCTTCCATA 
GACAGCGGCA TCAACATGGA CATACGCAGC AAGTCAGGCA AGTCCCTGGC CAGGCTGCAC 
AGCAACTTTG AGGGGCACGA CAGCTATGCA TTGGGAGTGG ACTCGCCGCT GGATACGCTG 
ACTGGGACCA ATTGCCTGCT CTCACCGCTA CGTAAAATGG ATTTCGCCCT GTGCGAGGTA 
TCCACGGGCG AGTCGAGCCC CATACACGAC AAAGACATCG ATGGCGATGA CACCTCGACG 
AGCGCCACCG ATGTGACCAT AGGCAATGAC CTGGCCCTGG CCCTGGCCGA TGCAGTTGTA 
GGGGACTCGG ATGCCAAAGT GCTGACTCTG GCCGAACACA TTATAGCAGC AATGCCAGAG 
CGCATCAAAA ATGAAGCCGA TGAAATGATG GTGCTGGGCA GCCCCTTGAC TGAGCCACTG 
ACCTCGGAGT CCTCGGCCCT GACAGACAGC TTTATGGATC CCCTGCTGGA CTCGCTGCCC 
AATACGCATT TCGACAGCGA CTTCAACTTC GACTTCCACG ACCACAGCTA TCGGTACCAC 
GATGTGAGCA CTCCCTGCTC CAGCCTGAGT CCCGCCAGCT CGGGGCCGCT GCAGTCGCCG 
GCCAGCTACT CCATACTGGG CACAGATCCG TCTGTCAGCT CGCCCAGTCC GCCGCCGTCG 
ACCAAGCAGC TGACCGAGTT CCTGCACGCA TCCAGCCTGT CGCAGTATCC GTTCGAGGCG 
GACTTCTCCA AGCTGACGCT GACCGACACG GAGCAGCGGG AGCTGTACGA GGCGGCCAAG 
TGCATACAGA AGGCCTACCG CTCGTACAAG GGCCGGCAGA AGCTCGAGGA GCAGAACAAA 
GAGCGCACTG CCGCCATTGT CATCCAGAAC TATTACAGGC GGTACAAGCA GTACGCCTAT 
TACAGGCAGA TGACCAACGC CGCCCTGGTC ATCCAGCATG GCTATCGTTC CTATCGAAAG 
AACAAACGCT TCAAGAAGAG CGGCCTGGGC CAGGGCATGG CAGGGGGCAG CGATCACGGC 
AGCGTCAGCA GCAACTCTCA GTGCCTGTCC AGCTTCTACG ACCACTACAA GCAGGATCAG 
CAGCAGCTGC AGCAGCAGCA TGAGATGGGC TCGCAGCCGA GCACGCCCAA GGAAACCAGT 
CCCTCGGGTC CCTTGAAACG AACCTACTCG CAGTCCACCC AGAATCAAGC TGCCAGGAAG 
ATCCAACAGT TCATGAGACA ATCACGCATC AACATTTGGG ATGCCAATTT GACGACTTTG 
ACTACAGAGA GAGCGAGCCG AAAAAGAGAA GCTGGTGCAC CAACGCAGGG CGGAATACCT 
TCAAAACTTG CAGTATCAAG GGCAGCAGGA AATCTTGGCT TGCCTAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015040437.1
CDS	372..6605
Translation	MENVSDASCSASSLKLQRQQTQIKAKNVLIRSQSSGKLSRNEAESTTTATTTATAKTTKATTRTLKIGAMQQKQLQPPNGVNASNIILLRGTRNENGQIIIQNKQDILTLLNEQQQHQEKAAQAAQASTAITLNQLPTTTTVARKTIVQSSGSSTTSTSSTISIVASSSSGSSSGVGAKDAASNTILLQTPINASQLESVLKAQERTKQANGTAQTKLIERPFLLKHATRSLSSESNCDSKSPFVLQTLKRLEKSQSILVIRNSTSGSTSSMAVSPPVNVSASVSSNVLSVTRTSKAAKGMAGALHKLKAAATAAAAAGAGAGAAPSASSTGTTILRLGKSATTLAINGAGGSSKLEVAAAAATATTTSTANKLSNTVTSEQQQQQQSTTTQTNVPLGNDGEPIKLPDNLESLPRADSFPSQRHRWNTNEEIAAILISFDKHCEWQSKEVRTRPKSGSLLLYSRKKVRYRRDGYCWKKRKDGKTTREDHMKLKVQGTECIYGCYVHSAILPTFHRRCYWLLQNPDIVLVHYLNVPYPDDNKMAVIAPSITLWGDKKEWTKEELVSQLKPMLSTVNSDDDSDSGNDIEISTAETVESIVCQLMEKQRLSRQAALVKQLDCGCGDSGCADGKTCTHPVMRRTGGIIKSAVATSASEKRLNEGAFNGGTPNVVVASKLYSRWAERPRQRNDGMHLDNPHAQFHNATQQLPQESAIKFQIIPPQQQQQQQQQTGHGDGSYQQQYRGANQMLAARSNLVMQQQQQQQQQYHQQQQQHLSLQRFVASQSQNSAHLNHHHHRLQIANTTITATARDPSTTVAAAATAASASAAGGGSVLNGMDTELIEIQSHMTANKITSSVSSNGNGSGSNPSASAKQLAAQTNNELNVVNNNDPSNNNFMYNQQQQQLNTSSNSNNTQQQQQHYYKLQQTSATPNSGQLQLQNQTQTQTQTPTLVQPPVEAMCMSPEHRSGSSSSSNNNTASTSSAVNSHNTSPSTAIATTTSMAASISISTSTSGTSSTSSSLQSIIDGNQQQQITTTSTAATCDNLMSANALTELNGGSSSSSNNQATDGHNKSQSQSQSQPQTLSQNGCATFSASSDNSSQISASDESSSFGANNQNNSIHSIHSSHSSSSNSSEDEPQAGNAAETLSFFNETLDLSHEDIQRTLIANMQPYNTAAGEAAATTIEPLATNGSHSSSNSNGNPQEVEKKPSVGQEVEAEPEEDDTDDVFANLDAFDMLVEFPELDLDDKQALNNTALEQGSYLGQAAAQTQQPRKIHNICDFSPEWSYTEGGVKVLVAGPWTSSNGAGAYTVLFDAQPVPTQMVQEGVLRCYCPAHEAGFVTLQVACGGFLVSNSVMFEYKLSLLADAPFDASSSNDCLYKFTLLNRLSTIDDKLQLKTEQEPTTDHTALYLEPNFEEKLVAYCHRLTKHAWSMPSTVASWSVGLRGMTLLHLAAALGYAKLVGAMLNWRAENPHIILETELDALSQDVYGFTPLAWSCVRGHVECSLLLYKWNHNALKIKTQSQQTALDLANLKGHKHLLSQIFRLEKERCRKPHLRGGLANLSMNLGVETEAEESPPQTYNSFDLEILQRKHDGVFLRPGAVHSNQSPPNNGSRYSKRSSIDSGINMDIRSKSGKSLARLHSNFEGHDSYALGVDSPLDTLTGTNCLLSPLRKMDFALCEVSTGESSPIHDKDIDGDDTSTSATDVTIGNDLALALADAVVGDSDAKVLTLAEHIIAAMPERIKNEADEMMVLGSPLTEPLTSESSALTDSFMDPLLDSLPNTHFDSDFNFDFHDHSYRYHDVSTPCSSLSPASSGPLQSPASYSILGTDPSVSSPSPPPSTKQLTEFLHASSLSQYPFEADFSKLTLTDTEQRELYEAAKCIQKAYRSYKGRQKLEEQNKERTAAIVIQNYYRRYKQYAYYRQMTNAALVIQHGYRSYRKNKRFKKSGLGQGMAGGSDHGSVSSNSQCLSSFYDHYKQDQQQLQQQHEMGSQPSTPKETSPSGPLKRTYSQSTQNQAARKIQQFMRQSRINIWDANLTTLTTERASRKREAGAPTQGGIPSKLAVSRAAGNLGLPRK

Target ORF information:

RefSeq Version	XM_015184951.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant F (Dpse\GA30460), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015184951.1

ORF Insert Sequence:

ATGGAAAATG TGTCCGATGC CAGCTGCAGC GCCTCTTCCC TGAAACTCCA ACGGCAGCAA 
ACTCAAATCA AAGCCAAAAA TGTCCTAATC AGATCCCAGA GCAGCGGCAA GCTTAGCAGA 
AATGAGGCTG AATCCACAAC AACAGCAACA ACAACAGCAA CAGCGAAAAC CACAAAAGCC 
ACAACGAGGA CTCTCAAGAT CGGGGCCATG CAACAGAAGC AGCTACAGCC ACCAAATGGA 
GTCAACGCTA GCAACATCAT CCTGCTGCGG GGCACACGCA ACGAGAACGG CCAGATCATT 
ATCCAGAACA AGCAGGACAT CCTCACCCTG CTCAACGAGC AGCAGCAGCA CCAGGAGAAG 
GCAGCCCAGG CGGCCCAGGC GTCCACAGCC ATCACACTCA ATCAACTGCC AACGACCACC 
ACAGTGGCTC GCAAGACGAT TGTCCAGAGC TCGGGGAGCA GCACCACCAG CACCAGCTCC 
ACCATATCCA TCGTGGCCAG CAGCAGCAGC GGCAGCAGTA GCGGTGTTGG TGCCAAGGAT 
GCCGCTAGCA ACACGATCCT CCTGCAGACG CCCATCAATG CCAGCCAGCT GGAGAGCGTG 
CTGAAGGCCC AGGAGCGAAC GAAACAGGCG AACGGCACGG CACAGACCAA GCTCATAGAG 
AGGCCCTTCC TGCTGAAGCA CGCCACGCGG AGCCTCAGCT CGGAGAGTAA TTGTGACAGC 
AAATCGCCGT TCGTGCTGCA GACACTCAAG CGACTGGAGA AATCGCAATC CATTCTGGTC 
ATACGCAACT CCACATCAGG CAGCACTTCC TCAATGGCCG TTTCTCCGCC TGTGAATGTT 
TCGGCCTCCG TATCATCGAA TGTTTTAAGC GTGACACGCA CTTCAAAGGC GGCCAAGGGC 
ATGGCAGGTG CGCTGCACAA GCTCAAGGCG GCCGCAACGG CAGCAGCCGC AGCGGGAGCA 
GGAGCAGGGG CAGCACCATC CGCATCATCG ACAGGCACAA CCATACTGAG GCTAGGCAAA 
AGCGCCACCA CATTGGCCAT TAACGGAGCT GGGGGCAGCA GCAAACTGGA GGTCGCCGCC 
GCCGCCGCAA CAGCAACCAC AACGTCAACA GCAAATAAAT TGTCCAACAC GGTGACATCG 
GAGCAGCAGC AACAGCAACA ATCGACAACA ACGCAAACGA ATGTGCCACT TGGGAACGAT 
GGCGAACCTA TCAAACTACC CGACAATTTG GAGAGCCTGC CAAGAGCTGA CAGTTTTCCA 
TCACAGCGTC ATCGTTGGAA TACAAATGAG GAAATTGCTG CGATTCTTAT CAGCTTTGAC 
AAGCACTGCG AGTGGCAGTC CAAGGAGGTC CGAACCAGAC CCAAGAGCGG ATCCCTGCTG 
CTGTACTCGC GGAAGAAGGT GCGCTATCGC CGCGACGGCT ATTGCTGGAA GAAGCGCAAG 
GATGGCAAGA CGACGCGCGA GGATCACATG AAACTGAAAG TACAAGGCAC GGAGTGCATC 
TACGGCTGTT ATGTACACTC GGCCATATTG CCCACGTTCC ATCGCAGATG TTACTGGCTG 
TTGCAGAATC CGGACATTGT TTTGGTGCAC TATCTGAATG TGCCGTATCC GGATGATAAT 
AAAATGGCAG TGATTGCCCC CAGCATCACC CTGTGGGGCG ACAAGAAAGA GTGGACCAAG 
GAGGAGCTGG TCAGCCAGCT GAAGCCGATG CTCTCCACAG TCAACTCCGA TGATGACTCC 
GACTCGGGCA ACGACATTGA GATATCGACG GCCGAGACCG TGGAGTCGAT TGTGTGCCAG 
CTGATGGAGA AGCAGCGCTT GTCCCGCCAG GCGGCCCTCG TCAAGCAGCT CGACTGCGGC 
TGCGGGGACT CGGGCTGTGC CGATGGCAAG ACCTGCACGC ATCCCGTGAT GCGGCGCACG 
GGCGGCATCA TCAAGTCCGC GGTGGCCACA TCGGCCTCAG AGAAGCGACT CAACGAGGGG 
GCCTTCAACG GCGGCACGCC CAATGTGGTG GTGGCCAGCA AGCTATACTC CCGCTGGGCG 
GAGCGGCCGC GCCAGCGCAA CGATGGCATG CACCTGGACA ACCCGCACGC ACAGTTCCAC 
AATGCCACGC AACAGCTGCC CCAGGAATCG GCCATCAAGT TTCAGATCAT TCCTCCTCAA 
CAGCAGCAGC AGCAGCAACA ACAGACGGGA CATGGCGATG GCAGCTATCA GCAGCAATAT 
CGTGGTGCCA ATCAGATGCT GGCCGCAAGA AGCAATCTGG TGATGCAGCA GCAACAGCAA 
CAGCAGCAGC AATATCACCA GCAACAGCAG CAACACTTGA GTCTGCAACG ATTCGTGGCC 
AGTCAGAGCC AGAATTCGGC TCATTTGAAT CATCATCATC ATCGCCTGCA AATTGCCAAT 
ACAACAATCA CGGCCACAGC CAGAGATCCT TCCACAACAG TAGCAGCAGC AGCAACAGCA 
GCATCGGCAT CAGCAGCAGG AGGAGGCTCC GTCTTAAATG GAATGGATAC GGAACTAATT 
GAAATCCAAA GCCACATGAC TGCAAACAAA ATCACTAGCT CCGTCAGCAG CAATGGAAAT 
GGCAGCGGCA GCAATCCCAG TGCCAGTGCC AAGCAATTAG CAGCTCAAAC GAACAACGAA 
TTAAATGTCG TAAATAATAA CGACCCCAGC AACAATAACT TTATGTACAA TCAACAACAG 
CAGCAACTGA ATACTTCCAG CAACAGCAAC AACACACAGC AGCAGCAGCA ACATTATTAT 
AAATTGCAAC AGACATCAGC AACCCCAAAC AGTGGCCAAC TCCAACTCCA AAATCAAACC 
CAAACACAAA CACAAACCCC AACCCTTGTC CAACCCCCTG TTGAGGCCAT GTGTATGTCA 
CCTGAGCATC GCAGCGGCAG CAGCAGCAGC AGCAACAACA ATACGGCCTC AACGTCTTCC 
GCTGTCAACA GTCATAACAC CTCCCCCTCC ACAGCCATAG CCACAACCAC GTCCATGGCC 
GCATCTATAT CTATATCCAC ATCTACGTCC GGCACATCGT CCACCTCGAG TTCCTTACAA 
TCGATTATCG ATGGCAATCA GCAGCAACAA ATTACAACGA CATCGACGGC AGCAACTTGT 
GATAATTTAA TGAGCGCCAA TGCGCTCACT GAGCTGAATG GAGGGAGCAG CAGCAGCAGC 
AACAATCAGG CCACAGATGG CCACAACAAG AGTCAATCCC AATCGCAGTC TCAGCCGCAA 
ACTCTGAGCC AAAATGGTTG TGCAACATTC AGTGCTTCCA GTGATAATAG CAGCCAAATT 
AGCGCCAGCG ACGAGAGTAG CAGCTTTGGG GCCAACAATC AGAACAACAG CATACACAGC 
ATCCACAGCA GCCACAGCAG CAGCAGTAAC AGCAGCGAGG ACGAGCCCCA GGCGGGCAAT 
GCTGCCGAGA CCCTGAGCTT CTTCAACGAA ACCCTGGATC TATCCCATGA GGATATTCAA 
CGTACACTGA TAGCCAATAT GCAGCCGTAC AATACGGCTG CAGGCGAAGC AGCAGCGACA 
ACGATAGAAC CTTTGGCAAC AAACGGCAGC CACAGTAGCA GCAACAGCAA TGGCAATCCT 
CAAGAAGTGG AGAAGAAACC TTCAGTCGGC CAGGAAGTCG AAGCCGAACC TGAGGAGGAT 
GACACAGACG ATGTGTTTGC CAACCTGGAT GCCTTCGATA TGCTCGTGGA GTTCCCCGAG 
CTGGATCTGG ATGATAAGCA GGCCCTGAAC AACACGGCCT TGGAGCAGGG CTCCTATCTG 
GGACAGGCGG CCGCCCAAAC GCAGCAGCCC CGCAAGATCC ACAACATCTG CGACTTCAGT 
CCGGAGTGGT CGTACACCGA GGGCGGAGTC AAGGTACTTG TGGCCGGACC CTGGACGAGC 
AGCAATGGTG CTGGTGCCTA CACGGTGCTC TTCGATGCCC AGCCCGTGCC CACGCAGATG 
GTGCAGGAGG GAGTGCTCCG TTGCTACTGT CCTGCCCACG AGGCGGGCTT TGTGACGCTC 
CAGGTGGCCT GCGGCGGCTT TCTCGTGTCC AATTCGGTCA TGTTCGAGTA CAAGCTGTCG 
CTGCTGGCCG ATGCCCCGTT CGATGCGAGC AGCTCCAACG ATTGCCTGTA CAAATTCACG 
CTGCTCAATC GCCTCTCGAC CATCGACGAC AAGTTGCAGC TGAAGACGGA GCAGGAGCCG 
ACGACGGACC ACACGGCTCT CTATCTGGAG CCGAACTTTG AGGAGAAGCT GGTCGCGTAC 
TGCCACAGGC TGACGAAGCA TGCCTGGAGC ATGCCCAGCA CAGTGGCCAG CTGGAGCGTG 
GGCCTGCGGG GCATGACGCT GCTGCATCTG GCCGCCGCCT TGGGCTATGC CAAGCTGGTA 
GGCGCCATGC TCAATTGGCG GGCGGAGAAT CCACATATCA TACTCGAAAC GGAGCTGGAC 
GCCCTCAGTC AGGATGTGTA CGGTTTCACG CCGCTCGCCT GGTCCTGTGT GCGCGGCCAT 
GTGGAGTGTT CCCTGCTGCT CTACAAGTGG AATCACAATG CGCTGAAGAT CAAGACGCAG 
AGCCAGCAAA CGGCCCTGGA TTTGGCCAAC CTGAAGGGGC ACAAACATCT CCTGTCCCAG 
ATATTTCGAC TGGAGAAGGA GCGCTGCCGC AAGCCGCACT TGCGAGGCGG CCTGGCCAAT 
CTTTCAATGA ACCTAGGCGT TGAAACGGAA GCGGAGGAGT CGCCGCCGCA GACCTACAAC 
AGCTTTGACC TGGAGATACT CCAAAGAAAG CACGATGGCG TCTTCCTCAG ACCTGGGGCA 
GTGCATAGCA ATCAAAGTCC TCCCAACAAT GGCAGCCGGT ACTCCAAGCG CTCTTCCATA 
GACAGCGGCA TCAACATGGA CATACGCAGC AAGTCAGGCA AGTCCCTGGC CAGGCTGCAC 
AGCAACTTTG AGGGGCACGA CAGCTATGCA TTGGGAGTGG ACTCGCCGCT GGATACGCTG 
ACTGGGACCA ATTGCCTGCT CTCACCGCTA CGTAAAATGG ATTTCGCCCT GTGCGAGGTA 
TCCACGGGCG AGTCGAGCCC CATACACGAC AAAGACATCG ATGGCGATGA CACCTCGACG 
AGCGCCACCG ATGTGACCAT AGGCAATGAC CTGGCCCTGG CCCTGGCCGA TGCAGTTGTA 
GGGGACTCGG ATGCCAAAGT GCTGACTCTG GCCGAACACA TTATAGCAGC AATGCCAGAG 
CGCATCAAAA ATGAAGCCGA TGAAATGATG GTGCTGGGCA GCCCCTTGAC TGAGCCACTG 
ACCTCGGAGT CCTCGGCCCT GACAGACAGC TTTATGGATC CCCTGCTGGA CTCGCTGCCC 
AATACGCATT TCGACAGCGA CTTCAACTTC GACTTCCACG ACCACAGCTA TCGGTACCAC 
GATGTGAGCA CTCCCTGCTC CAGCCTGAGT CCCGCCAGCT CGGGGCCGCT GCAGTCGCCG 
GCCAGCTACT CCATACTGGG CACAGATCCG TCTGTCAGCT CGCCCAGTCC GCCGCCGTCG 
ACCAAGCAGC TGACCGAGTT CCTGCACGCA TCCAGCCTGT CGCAGTATCC GTTCGAGGCG 
GACTTCTCCA AGCTGACGCT GACCGACACG GAGCAGCGGG AGCTGTACGA GGCGGCCAAG 
TGCATACAGA AGGCCTACCG CTCGTACAAG GGCCGGCAGA AGCTCGAGGA GCAGAACAAA 
GAGCGCACTG CCGCCATTGT CATCCAGAAC TATTACAGGC GGTACAAGCA GTACGCCTAT 
TACAGGCAGA TGACCAACGC CGCCCTGGTC ATCCAGCATG GCTATCGTTC CTATCGAAAG 
AACAAACGCT TCAAGAAGAG CGGCCTGGGC CAGGGCATGG CAGGGGGCAG CGATCACGGC 
AGCGTCAGCA GCAACTCTCA GTGCCTGTCC AGCTTCTACG ACCACTACAA GCAGGATCAG 
CAGCAGCTGC AGCAGCAGCA TGAGATGGGC TCGCAGCCGA GCACGCCCAA GGAAACCAGT 
CCCTCGGGTC CCTTGAAACG AACCTACTCG CAGTCCACCC AGAATCAAGC TGCCAGGAAG 
ATCCAACAGT TCATGAGACA ATCACGCATC AACATTTGGG ATGCCAATTT GACGACTTTG 
ACTACAGAGA GAGCGAGCCG AAAAAGAGAA GCTGGTGCAC CAACGCAGGG CGGAATACCT 
TCAAAACTTG CAGTATCAAG GGCAGCAGGA AATCTTGGCT TGCCTAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA30460 ( XM_015184952.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015040438.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant G (Dpse\GA30460), mRNA.

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CloneID	ODe23358
Clone ID Related Accession (Same CDS sequence)	XM_015184952.1
Accession Version	XM_015184952.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform G
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAATG TGTCCGATGC CAGCTGCAGC GCCTCTTCCC TGAAACTCCA ACGGCAGCAA 
ACTCAAATCA AAGCCAAAAA TGTCCTAATC AGATCCCAGA GCAGCGGCAA GCTTAGCAGA 
AATGAGGCTG AATCCACAAC AACAGCAACA ACAACAGCAA CAGCGAAAAC CACAAAAGCC 
ACAACGAGGA CTCTCAAGAT CGGGGCCATG CAACAGAAGC AGCTACAGCC ACCAAATGGA 
GTCAACGCTA GCAACATCAT CCTGCTGCGG GGCACACGCA ACGAGAACGG CCAGATCATT 
ATCCAGAACA AGCAGGACAT CCTCACCCTG CTCAACGAGC AGCAGCAGCA CCAGGAGAAG 
GCAGCCCAGG CGGCCCAGGC GTCCACAGCC ATCACACTCA ATCAACTGCC AACGACCACC 
ACAGTGGCTC GCAAGACGAT TGTCCAGAGC TCGGGGAGCA GCACCACCAG CACCAGCTCC 
ACCATATCCA TCGTGGCCAG CAGCAGCAGC GGCAGCAGTA GCGGTGTTGG TGCCAAGGAT 
GCCGCTAGCA ACACGATCCT CCTGCAGACG CCCATCAATG CCAGCCAGCT GGAGAGCGTG 
CTGAAGGCCC AGGAGCGAAC GAAACAGGCG AACGGCACGG CACAGACCAA GCTCATAGAG 
AGGCCCTTCC TGCTGAAGCA CGCCACGCGG AGCCTCAGCT CGGAGAGTAA TTGTGACAGC 
AAATCGCCGT TCGTGCTGCA GACACTCAAG CGACTGGAGA AATCGCAATC CATTCTGGTC 
ATACGCAACT CCACATCAGG CAGCACTTCC TCAATGGCCG TTTCTCCGCC TGTGAATGTT 
TCGGCCTCCG TATCATCGAA TGTTTTAAGC GTGACACGCA CTTCAAAGGC GGCCAAGGGC 
ATGGCAGGTG CGCTGCACAA GCTCAAGGCG GCCGCAACGG CAGCAGCCGC AGCGGGAGCA 
GGAGCAGGGG CAGCACCATC CGCATCATCG ACAGGCACAA CCATACTGAG GCTAGGCAAA 
AGCGCCACCA CATTGGCCAT TAACGGAGCT GGGGGCAGCA GCAAACTGGA GGTCGCCGCC 
GCCGCCGCAA CAGCAACCAC AACGTCAACA GCAAATAAAT TGTCCAACAC GGTGACATCG 
GAGCAGCAGC AACAGCAACA ATCGACAACA ACGCAAACGA ATGTGCCACT TGGGAACGAT 
GGCGAACCTA TCAAACTACC CGACAATTTG GAGAGCCTGC CAAGAGCTGA CAGTTTTCCA 
TCACAGCGTC ATCGTTGGAA TACAAATGAG GAAATTGCTG CGATTCTTAT CAGCTTTGAC 
AAGCACTGCG AGTGGCAGTC CAAGGAGGTC CGAACCAGAC CCAAGAGCGG ATCCCTGCTG 
CTGTACTCGC GGAAGAAGGT GCGCTATCGC CGCGACGGCT ATTGCTGGAA GAAGCGCAAG 
GATGGCAAGA CGACGCGCGA GGATCACATG AAACTGAAAG TACAAGGCAC GGAGTGCATC 
TACGGCTGTT ATGTACACTC GGCCATATTG CCCACGTTCC ATCGCAGATG TTACTGGCTG 
TTGCAGAATC CGGACATTGT TTTGGTGCAC TATCTGAATG TGCCGTATCC GGATGATAAT 
AAAATGGCAG TGATTGCCCC CAGCATCACC CTGTGGGGCG ACAAGAAAGA GTGGACCAAG 
GAGGAGCTGG TCAGCCAGCT GAAGCCGATG CTCTCCACAG TCAACTCCGA TGATGACTCC 
GACTCGGGCA ACGACATTGA GATATCGACG GCCGAGACCG TGGAGTCGAT TGTGTGCCAG 
CTGATGGAGA AGCAGCGCTT GTCCCGCCAG GCGGCCCTCG TCAAGCAGCT CGACTGCGGC 
TGCGGGGACT CGGGCTGTGC CGATGGCAAG ACCTGCACGC ATCCCGTGAT GCGGCGCACG 
GGCGGCATCA TCAAGTCCGC GGTGGCCACA TCGGCCTCAG AGAAGCGACT CAACGAGGGG 
GCCTTCAACG GCGGCACGCC CAATGTGGTG GTGGCCAGCA AGCTATACTC CCGCTGGGCG 
GAGCGGCCGC GCCAGCGCAA CGATGGCATG CACCTGGACA ACCCGCACGC ACAGTTCCAC 
AATGCCACGC AACAGCTGCC CCAGGAATCG GCCATCAAGT TTCAGATCAT TCCTCCTCAA 
CAGCAGCAGC AGCAGCAACA ACAGACGGGA CATGGCGATG GCAGCTATCA GCAGCAATAT 
CGTGGTGCCA ATCAGATGCT GGCCGCAAGA AGCAATCTGG TGATGCAGCA GCAACAGCAA 
CAGCAGCAGC AATATCACCA GCAACAGCAG CAACACTTGA GTCTGCAACG ATTCGTGGCC 
AGTCAGAGCC AGAATTCGGC TCATTTGAAT CATCATCATC ATCGCCTGCA AATTGCCAAT 
ACAACAATCA CGGCCACAGC CAGAGATCCT TCCACAACAG TAGCAGCAGC AGCAACAGCA 
GCATCGGCAT CAGCAGCAGG AGGAGGCTCC GTCTTAAATG GAATGGATAC GGAACTAATT 
GAAATCCAAA GCCACATGAC TGCAAACAAA ATCACTAGCT CCGTCAGCAG CAATGGAAAT 
GGCAGCGGCA GCAATCCCAG TGCCAGTGCC AAGCAATTAG CAGCTCAAAC GAACAACGAA 
TTAAATGTCG TAAATAATAA CGACCCCAGC AACAATAACT TTATGTACAA TCAACAACAG 
CAGCAACTGA ATACTTCCAG CAACAGCAAC AACACACAGC AGCAGCAGCA ACATTATTAT 
AAATTGCAAC AGACATCAGC AACCCCAAAC AGTGGCCAAC TCCAACTCCA AAATCAAACC 
CAAACACAAA CACAAACCCC AACCCTTGTC CAACCCCCTG TTGAGGCCAT GTGTATGTCA 
CCTGAGCATC GCAGCGGCAG CAGCAGCAGC AGCAACAACA ATACGGCCTC AACGTCTTCC 
GCTGTCAACA GTCATAACAC CTCCCCCTCC ACAGCCATAG CCACAACCAC GTCCATGGCC 
GCATCTATAT CTATATCCAC ATCTACGTCC GGCACATCGT CCACCTCGAG TTCCTTACAA 
TCGATTATCG ATGGCAATCA GCAGCAACAA ATTACAACGA CATCGACGGC AGCAACTTGT 
GATAATTTAA TGAGCGCCAA TGCGCTCACT GAGCTGAATG GAGGGAGCAG CAGCAGCAGC 
AACAATCAGG CCACAGATGG CCACAACAAG AGTCAATCCC AATCGCAGTC TCAGCCGCAA 
ACTCTGAGCC AAAATGGTTG TGCAACATTC AGTGCTTCCA GTGATAATAG CAGCCAAATT 
AGCGCCAGCG ACGAGAGTAG CAGCTTTGGG GCCAACAATC AGAACAACAG CATACACAGC 
ATCCACAGCA GCCACAGCAG CAGCAGTAAC AGCAGCGAGG ACGAGCCCCA GGCGGGCAAT 
GCTGCCGAGA CCCTGAGCTT CTTCAACGAA ACCCTGGATC TATCCCATGA GGATATTCAA 
CGTACACTGA TAGCCAATAT GCAGCCGTAC AATACGGCTG CAGGCGAAGC AGCAGCGACA 
ACGATAGAAC CTTTGGCAAC AAACGGCAGC CACAGTAGCA GCAACAGCAA TGGCAATCCT 
CAAGAAGTGG AGAAGAAACC TTCAGTCGGC CAGGAAGTCG AAGCCGAACC TGAGGAGGAT 
GACACAGACG ATGTGTTTGC CAACCTGGAT GCCTTCGATA TGCTCGTGGA GTTCCCCGAG 
CTGGATCTGG ATGATAAGCA GGCCCTGAAC AACACGGCCT TGGAGCAGGG CTCCTATCTG 
GGACAGGCGG CCGCCCAAAC GCAGCAGCCC CGCAAGATCC ACAACATCTG CGACTTCAGT 
CCGGAGTGGT CGTACACCGA GGGCGGAGTC AAGGTACTTG TGGCCGGACC CTGGACGAGC 
AGCAATGGTG CTGGTGCCTA CACGGTGCTC TTCGATGCCC AGCCCGTGCC CACGCAGATG 
GTGCAGGAGG GAGTGCTCCG TTGCTACTGT CCTGCCCACG AGGCGGGCTT TGTGACGCTC 
CAGGTGGCCT GCGGCGGCTT TCTCGTGTCC AATTCGGTCA TGTTCGAGTA CAAGCTGTCG 
CTGCTGGCCG ATGCCCCGTT CGATGCGAGC AGCTCCAACG ATTGCCTGTA CAAATTCACG 
CTGCTCAATC GCCTCTCGAC CATCGACGAC AAGTTGCAGC TGAAGACGGA GCAGGAGCCG 
ACGACGGACC ACACGGCTCT CTATCTGGAG CCGAACTTTG AGGAGAAGCT GGTCGCGTAC 
TGCCACAGGC TGACGAAGCA TGCCTGGAGC ATGCCCAGCA CAGTGGCCAG CTGGAGCGTG 
GGCCTGCGGG GCATGACGCT GCTGCATCTG GCCGCCGCCT TGGGCTATGC CAAGCTGGTA 
GGCGCCATGC TCAATTGGCG GGCGGAGAAT CCACATATCA TACTCGAAAC GGAGCTGGAC 
GCCCTCAGTC AGGATGTGTA CGGTTTCACG CCGCTCGCCT GGTCCTGTGT GCGCGGCCAT 
GTGGAGTGTT CCCTGCTGCT CTACAAGTGG AATCACAATG CGCTGAAGAT CAAGACGCAG 
AGCCAGCAAA CGGCCCTGGA TTTGGCCAAC CTGAAGGGGC ACAAACATCT CCTGTCCCAG 
ATATTTCGAC TGGAGAAGGA GCGCTGCCGC AAGCCGCACT TGCGAGGCGG CCTGGCCAAT 
CTTTCAATGA ACCTAGGCGT TGAAACGGAA GCGGAGGAGT CGCCGCCGCA GACCTACAAC 
AGCTTTGACC TGGAGATACT CCAAAGAAAG CACGATGGCG TCTTCCTCAG ACCTGGGGCA 
GTGCATAGCA ATCAAAGTCC TCCCAACAAT GGCAGCCGGT ACTCCAAGCG CTCTTCCATA 
GACAGCGGCA TCAACATGGA CATACGCAGC AAGTCAGGCA AGTCCCTGGC CAGGCTGCAC 
AGCAACTTTG AGGGGCACGA CAGCTATGCA TTGGGAGTGG ACTCGCCGCT GGATACGCTG 
ACTGGGACCA ATTGCCTGCT CTCACCGCTA CGTAAAATGG ATTTCGCCCT GTGGGACTCG 
GATGCCAAAG TGCTGACTCT GGCCGAACAC ATTATAGCAG CAATGCCAGA GCGCATCAAA 
AATGAAGCCG ATGAAATGAT GGTGCTGGGC AGCCCCTTGA CTGAGCCACT GACCTCGGAG 
TCCTCGGCCC TGACAGACAG CTTTATGGAT CCCCTGCTGG ACTCGCTGCC CAATACGCAT 
TTCGACAGCG ACTTCAACTT CGACTTCCAC GACCACAGCT ATCGGTACCA CGATGTGAGC 
ACTCCCTGCT CCAGCCTGAG TCCCGCCAGC TCGGGGCCGC TGCAGTCGCC GGCCAGCTAC 
TCCATACTGG GCACAGATCC GTCTGTCAGC TCGCCCAGTC CGCCGCCGTC GACCAAGCAG 
CTGACCGAGT TCCTGCACGC ATCCAGCCTG TCGCAGTATC CGTTCGAGGC GGACTTCTCC 
AAGCTGACGC TGACCGACAC GGAGCAGCGG GAGCTGTACG AGGCGGCCAA GTGCATACAG 
AAGGCCTACC GCTCGTACAA GGGCCGGCAG AAGCTCGAGG AGCAGAACAA AGAGCGCACT 
GCCGCCATTG TCATCCAGAA CTATTACAGG CGGTACAAGC AGTACGCCTA TTACAGGCAG 
ATGACCAACG CCGCCCTGGT CATCCAGCAT GGCTATCGTT CCTATCGAAA GAACAAACGC 
TTCAAGAAGA GCGGCCTGGG CCAGGGCATG GCAGGGGGCA GCGATCACGG CAGCGTCAGC 
AGCAACTCTC AGTGCCTGTC CAGCTTCTAC GACCACTACA AGCAGGATCA GCAGCAGCTG 
CAGCAGCAGC ATGAGATGGG CTCGCAGCCG AGCACGCCCA AGGAAACCAG TCCCTCGGGT 
CCCTTGAAAC GAACCTACTC GCAGTCCACC CAGAATCAAG CTGCCAGGAA GATCCAACAG 
TTCATGAGAC AATCACGCAT CAAACTACAG AGAGAGCGAG CCGAAAAAGA GAAGCTGGTG 
CACCAACGCA GGGCGGAATA CCTTCAAAAC TTGCAGTATC AAGGGCAGCA GGAAATCTTG 
GCTTGCCTAG AAAATAACAG TTCTGCCCAG AGCAGTGGCA GCAACACCTA TACGAACAGC 
ATCAACAATC TACAATCCAA TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015040438.1
CDS	371..6517
Translation	MENVSDASCSASSLKLQRQQTQIKAKNVLIRSQSSGKLSRNEAESTTTATTTATAKTTKATTRTLKIGAMQQKQLQPPNGVNASNIILLRGTRNENGQIIIQNKQDILTLLNEQQQHQEKAAQAAQASTAITLNQLPTTTTVARKTIVQSSGSSTTSTSSTISIVASSSSGSSSGVGAKDAASNTILLQTPINASQLESVLKAQERTKQANGTAQTKLIERPFLLKHATRSLSSESNCDSKSPFVLQTLKRLEKSQSILVIRNSTSGSTSSMAVSPPVNVSASVSSNVLSVTRTSKAAKGMAGALHKLKAAATAAAAAGAGAGAAPSASSTGTTILRLGKSATTLAINGAGGSSKLEVAAAAATATTTSTANKLSNTVTSEQQQQQQSTTTQTNVPLGNDGEPIKLPDNLESLPRADSFPSQRHRWNTNEEIAAILISFDKHCEWQSKEVRTRPKSGSLLLYSRKKVRYRRDGYCWKKRKDGKTTREDHMKLKVQGTECIYGCYVHSAILPTFHRRCYWLLQNPDIVLVHYLNVPYPDDNKMAVIAPSITLWGDKKEWTKEELVSQLKPMLSTVNSDDDSDSGNDIEISTAETVESIVCQLMEKQRLSRQAALVKQLDCGCGDSGCADGKTCTHPVMRRTGGIIKSAVATSASEKRLNEGAFNGGTPNVVVASKLYSRWAERPRQRNDGMHLDNPHAQFHNATQQLPQESAIKFQIIPPQQQQQQQQQTGHGDGSYQQQYRGANQMLAARSNLVMQQQQQQQQQYHQQQQQHLSLQRFVASQSQNSAHLNHHHHRLQIANTTITATARDPSTTVAAAATAASASAAGGGSVLNGMDTELIEIQSHMTANKITSSVSSNGNGSGSNPSASAKQLAAQTNNELNVVNNNDPSNNNFMYNQQQQQLNTSSNSNNTQQQQQHYYKLQQTSATPNSGQLQLQNQTQTQTQTPTLVQPPVEAMCMSPEHRSGSSSSSNNNTASTSSAVNSHNTSPSTAIATTTSMAASISISTSTSGTSSTSSSLQSIIDGNQQQQITTTSTAATCDNLMSANALTELNGGSSSSSNNQATDGHNKSQSQSQSQPQTLSQNGCATFSASSDNSSQISASDESSSFGANNQNNSIHSIHSSHSSSSNSSEDEPQAGNAAETLSFFNETLDLSHEDIQRTLIANMQPYNTAAGEAAATTIEPLATNGSHSSSNSNGNPQEVEKKPSVGQEVEAEPEEDDTDDVFANLDAFDMLVEFPELDLDDKQALNNTALEQGSYLGQAAAQTQQPRKIHNICDFSPEWSYTEGGVKVLVAGPWTSSNGAGAYTVLFDAQPVPTQMVQEGVLRCYCPAHEAGFVTLQVACGGFLVSNSVMFEYKLSLLADAPFDASSSNDCLYKFTLLNRLSTIDDKLQLKTEQEPTTDHTALYLEPNFEEKLVAYCHRLTKHAWSMPSTVASWSVGLRGMTLLHLAAALGYAKLVGAMLNWRAENPHIILETELDALSQDVYGFTPLAWSCVRGHVECSLLLYKWNHNALKIKTQSQQTALDLANLKGHKHLLSQIFRLEKERCRKPHLRGGLANLSMNLGVETEAEESPPQTYNSFDLEILQRKHDGVFLRPGAVHSNQSPPNNGSRYSKRSSIDSGINMDIRSKSGKSLARLHSNFEGHDSYALGVDSPLDTLTGTNCLLSPLRKMDFALWDSDAKVLTLAEHIIAAMPERIKNEADEMMVLGSPLTEPLTSESSALTDSFMDPLLDSLPNTHFDSDFNFDFHDHSYRYHDVSTPCSSLSPASSGPLQSPASYSILGTDPSVSSPSPPPSTKQLTEFLHASSLSQYPFEADFSKLTLTDTEQRELYEAAKCIQKAYRSYKGRQKLEEQNKERTAAIVIQNYYRRYKQYAYYRQMTNAALVIQHGYRSYRKNKRFKKSGLGQGMAGGSDHGSVSSNSQCLSSFYDHYKQDQQQLQQQHEMGSQPSTPKETSPSGPLKRTYSQSTQNQAARKIQQFMRQSRIKLQRERAEKEKLVHQRRAEYLQNLQYQGQQEILACLENNSSAQSSGSNTYTNSINNLQSNQ

Target ORF information:

RefSeq Version	XM_015184952.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant G (Dpse\GA30460), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015184952.1

ORF Insert Sequence:

ATGGAAAATG TGTCCGATGC CAGCTGCAGC GCCTCTTCCC TGAAACTCCA ACGGCAGCAA 
ACTCAAATCA AAGCCAAAAA TGTCCTAATC AGATCCCAGA GCAGCGGCAA GCTTAGCAGA 
AATGAGGCTG AATCCACAAC AACAGCAACA ACAACAGCAA CAGCGAAAAC CACAAAAGCC 
ACAACGAGGA CTCTCAAGAT CGGGGCCATG CAACAGAAGC AGCTACAGCC ACCAAATGGA 
GTCAACGCTA GCAACATCAT CCTGCTGCGG GGCACACGCA ACGAGAACGG CCAGATCATT 
ATCCAGAACA AGCAGGACAT CCTCACCCTG CTCAACGAGC AGCAGCAGCA CCAGGAGAAG 
GCAGCCCAGG CGGCCCAGGC GTCCACAGCC ATCACACTCA ATCAACTGCC AACGACCACC 
ACAGTGGCTC GCAAGACGAT TGTCCAGAGC TCGGGGAGCA GCACCACCAG CACCAGCTCC 
ACCATATCCA TCGTGGCCAG CAGCAGCAGC GGCAGCAGTA GCGGTGTTGG TGCCAAGGAT 
GCCGCTAGCA ACACGATCCT CCTGCAGACG CCCATCAATG CCAGCCAGCT GGAGAGCGTG 
CTGAAGGCCC AGGAGCGAAC GAAACAGGCG AACGGCACGG CACAGACCAA GCTCATAGAG 
AGGCCCTTCC TGCTGAAGCA CGCCACGCGG AGCCTCAGCT CGGAGAGTAA TTGTGACAGC 
AAATCGCCGT TCGTGCTGCA GACACTCAAG CGACTGGAGA AATCGCAATC CATTCTGGTC 
ATACGCAACT CCACATCAGG CAGCACTTCC TCAATGGCCG TTTCTCCGCC TGTGAATGTT 
TCGGCCTCCG TATCATCGAA TGTTTTAAGC GTGACACGCA CTTCAAAGGC GGCCAAGGGC 
ATGGCAGGTG CGCTGCACAA GCTCAAGGCG GCCGCAACGG CAGCAGCCGC AGCGGGAGCA 
GGAGCAGGGG CAGCACCATC CGCATCATCG ACAGGCACAA CCATACTGAG GCTAGGCAAA 
AGCGCCACCA CATTGGCCAT TAACGGAGCT GGGGGCAGCA GCAAACTGGA GGTCGCCGCC 
GCCGCCGCAA CAGCAACCAC AACGTCAACA GCAAATAAAT TGTCCAACAC GGTGACATCG 
GAGCAGCAGC AACAGCAACA ATCGACAACA ACGCAAACGA ATGTGCCACT TGGGAACGAT 
GGCGAACCTA TCAAACTACC CGACAATTTG GAGAGCCTGC CAAGAGCTGA CAGTTTTCCA 
TCACAGCGTC ATCGTTGGAA TACAAATGAG GAAATTGCTG CGATTCTTAT CAGCTTTGAC 
AAGCACTGCG AGTGGCAGTC CAAGGAGGTC CGAACCAGAC CCAAGAGCGG ATCCCTGCTG 
CTGTACTCGC GGAAGAAGGT GCGCTATCGC CGCGACGGCT ATTGCTGGAA GAAGCGCAAG 
GATGGCAAGA CGACGCGCGA GGATCACATG AAACTGAAAG TACAAGGCAC GGAGTGCATC 
TACGGCTGTT ATGTACACTC GGCCATATTG CCCACGTTCC ATCGCAGATG TTACTGGCTG 
TTGCAGAATC CGGACATTGT TTTGGTGCAC TATCTGAATG TGCCGTATCC GGATGATAAT 
AAAATGGCAG TGATTGCCCC CAGCATCACC CTGTGGGGCG ACAAGAAAGA GTGGACCAAG 
GAGGAGCTGG TCAGCCAGCT GAAGCCGATG CTCTCCACAG TCAACTCCGA TGATGACTCC 
GACTCGGGCA ACGACATTGA GATATCGACG GCCGAGACCG TGGAGTCGAT TGTGTGCCAG 
CTGATGGAGA AGCAGCGCTT GTCCCGCCAG GCGGCCCTCG TCAAGCAGCT CGACTGCGGC 
TGCGGGGACT CGGGCTGTGC CGATGGCAAG ACCTGCACGC ATCCCGTGAT GCGGCGCACG 
GGCGGCATCA TCAAGTCCGC GGTGGCCACA TCGGCCTCAG AGAAGCGACT CAACGAGGGG 
GCCTTCAACG GCGGCACGCC CAATGTGGTG GTGGCCAGCA AGCTATACTC CCGCTGGGCG 
GAGCGGCCGC GCCAGCGCAA CGATGGCATG CACCTGGACA ACCCGCACGC ACAGTTCCAC 
AATGCCACGC AACAGCTGCC CCAGGAATCG GCCATCAAGT TTCAGATCAT TCCTCCTCAA 
CAGCAGCAGC AGCAGCAACA ACAGACGGGA CATGGCGATG GCAGCTATCA GCAGCAATAT 
CGTGGTGCCA ATCAGATGCT GGCCGCAAGA AGCAATCTGG TGATGCAGCA GCAACAGCAA 
CAGCAGCAGC AATATCACCA GCAACAGCAG CAACACTTGA GTCTGCAACG ATTCGTGGCC 
AGTCAGAGCC AGAATTCGGC TCATTTGAAT CATCATCATC ATCGCCTGCA AATTGCCAAT 
ACAACAATCA CGGCCACAGC CAGAGATCCT TCCACAACAG TAGCAGCAGC AGCAACAGCA 
GCATCGGCAT CAGCAGCAGG AGGAGGCTCC GTCTTAAATG GAATGGATAC GGAACTAATT 
GAAATCCAAA GCCACATGAC TGCAAACAAA ATCACTAGCT CCGTCAGCAG CAATGGAAAT 
GGCAGCGGCA GCAATCCCAG TGCCAGTGCC AAGCAATTAG CAGCTCAAAC GAACAACGAA 
TTAAATGTCG TAAATAATAA CGACCCCAGC AACAATAACT TTATGTACAA TCAACAACAG 
CAGCAACTGA ATACTTCCAG CAACAGCAAC AACACACAGC AGCAGCAGCA ACATTATTAT 
AAATTGCAAC AGACATCAGC AACCCCAAAC AGTGGCCAAC TCCAACTCCA AAATCAAACC 
CAAACACAAA CACAAACCCC AACCCTTGTC CAACCCCCTG TTGAGGCCAT GTGTATGTCA 
CCTGAGCATC GCAGCGGCAG CAGCAGCAGC AGCAACAACA ATACGGCCTC AACGTCTTCC 
GCTGTCAACA GTCATAACAC CTCCCCCTCC ACAGCCATAG CCACAACCAC GTCCATGGCC 
GCATCTATAT CTATATCCAC ATCTACGTCC GGCACATCGT CCACCTCGAG TTCCTTACAA 
TCGATTATCG ATGGCAATCA GCAGCAACAA ATTACAACGA CATCGACGGC AGCAACTTGT 
GATAATTTAA TGAGCGCCAA TGCGCTCACT GAGCTGAATG GAGGGAGCAG CAGCAGCAGC 
AACAATCAGG CCACAGATGG CCACAACAAG AGTCAATCCC AATCGCAGTC TCAGCCGCAA 
ACTCTGAGCC AAAATGGTTG TGCAACATTC AGTGCTTCCA GTGATAATAG CAGCCAAATT 
AGCGCCAGCG ACGAGAGTAG CAGCTTTGGG GCCAACAATC AGAACAACAG CATACACAGC 
ATCCACAGCA GCCACAGCAG CAGCAGTAAC AGCAGCGAGG ACGAGCCCCA GGCGGGCAAT 
GCTGCCGAGA CCCTGAGCTT CTTCAACGAA ACCCTGGATC TATCCCATGA GGATATTCAA 
CGTACACTGA TAGCCAATAT GCAGCCGTAC AATACGGCTG CAGGCGAAGC AGCAGCGACA 
ACGATAGAAC CTTTGGCAAC AAACGGCAGC CACAGTAGCA GCAACAGCAA TGGCAATCCT 
CAAGAAGTGG AGAAGAAACC TTCAGTCGGC CAGGAAGTCG AAGCCGAACC TGAGGAGGAT 
GACACAGACG ATGTGTTTGC CAACCTGGAT GCCTTCGATA TGCTCGTGGA GTTCCCCGAG 
CTGGATCTGG ATGATAAGCA GGCCCTGAAC AACACGGCCT TGGAGCAGGG CTCCTATCTG 
GGACAGGCGG CCGCCCAAAC GCAGCAGCCC CGCAAGATCC ACAACATCTG CGACTTCAGT 
CCGGAGTGGT CGTACACCGA GGGCGGAGTC AAGGTACTTG TGGCCGGACC CTGGACGAGC 
AGCAATGGTG CTGGTGCCTA CACGGTGCTC TTCGATGCCC AGCCCGTGCC CACGCAGATG 
GTGCAGGAGG GAGTGCTCCG TTGCTACTGT CCTGCCCACG AGGCGGGCTT TGTGACGCTC 
CAGGTGGCCT GCGGCGGCTT TCTCGTGTCC AATTCGGTCA TGTTCGAGTA CAAGCTGTCG 
CTGCTGGCCG ATGCCCCGTT CGATGCGAGC AGCTCCAACG ATTGCCTGTA CAAATTCACG 
CTGCTCAATC GCCTCTCGAC CATCGACGAC AAGTTGCAGC TGAAGACGGA GCAGGAGCCG 
ACGACGGACC ACACGGCTCT CTATCTGGAG CCGAACTTTG AGGAGAAGCT GGTCGCGTAC 
TGCCACAGGC TGACGAAGCA TGCCTGGAGC ATGCCCAGCA CAGTGGCCAG CTGGAGCGTG 
GGCCTGCGGG GCATGACGCT GCTGCATCTG GCCGCCGCCT TGGGCTATGC CAAGCTGGTA 
GGCGCCATGC TCAATTGGCG GGCGGAGAAT CCACATATCA TACTCGAAAC GGAGCTGGAC 
GCCCTCAGTC AGGATGTGTA CGGTTTCACG CCGCTCGCCT GGTCCTGTGT GCGCGGCCAT 
GTGGAGTGTT CCCTGCTGCT CTACAAGTGG AATCACAATG CGCTGAAGAT CAAGACGCAG 
AGCCAGCAAA CGGCCCTGGA TTTGGCCAAC CTGAAGGGGC ACAAACATCT CCTGTCCCAG 
ATATTTCGAC TGGAGAAGGA GCGCTGCCGC AAGCCGCACT TGCGAGGCGG CCTGGCCAAT 
CTTTCAATGA ACCTAGGCGT TGAAACGGAA GCGGAGGAGT CGCCGCCGCA GACCTACAAC 
AGCTTTGACC TGGAGATACT CCAAAGAAAG CACGATGGCG TCTTCCTCAG ACCTGGGGCA 
GTGCATAGCA ATCAAAGTCC TCCCAACAAT GGCAGCCGGT ACTCCAAGCG CTCTTCCATA 
GACAGCGGCA TCAACATGGA CATACGCAGC AAGTCAGGCA AGTCCCTGGC CAGGCTGCAC 
AGCAACTTTG AGGGGCACGA CAGCTATGCA TTGGGAGTGG ACTCGCCGCT GGATACGCTG 
ACTGGGACCA ATTGCCTGCT CTCACCGCTA CGTAAAATGG ATTTCGCCCT GTGGGACTCG 
GATGCCAAAG TGCTGACTCT GGCCGAACAC ATTATAGCAG CAATGCCAGA GCGCATCAAA 
AATGAAGCCG ATGAAATGAT GGTGCTGGGC AGCCCCTTGA CTGAGCCACT GACCTCGGAG 
TCCTCGGCCC TGACAGACAG CTTTATGGAT CCCCTGCTGG ACTCGCTGCC CAATACGCAT 
TTCGACAGCG ACTTCAACTT CGACTTCCAC GACCACAGCT ATCGGTACCA CGATGTGAGC 
ACTCCCTGCT CCAGCCTGAG TCCCGCCAGC TCGGGGCCGC TGCAGTCGCC GGCCAGCTAC 
TCCATACTGG GCACAGATCC GTCTGTCAGC TCGCCCAGTC CGCCGCCGTC GACCAAGCAG 
CTGACCGAGT TCCTGCACGC ATCCAGCCTG TCGCAGTATC CGTTCGAGGC GGACTTCTCC 
AAGCTGACGC TGACCGACAC GGAGCAGCGG GAGCTGTACG AGGCGGCCAA GTGCATACAG 
AAGGCCTACC GCTCGTACAA GGGCCGGCAG AAGCTCGAGG AGCAGAACAA AGAGCGCACT 
GCCGCCATTG TCATCCAGAA CTATTACAGG CGGTACAAGC AGTACGCCTA TTACAGGCAG 
ATGACCAACG CCGCCCTGGT CATCCAGCAT GGCTATCGTT CCTATCGAAA GAACAAACGC 
TTCAAGAAGA GCGGCCTGGG CCAGGGCATG GCAGGGGGCA GCGATCACGG CAGCGTCAGC 
AGCAACTCTC AGTGCCTGTC CAGCTTCTAC GACCACTACA AGCAGGATCA GCAGCAGCTG 
CAGCAGCAGC ATGAGATGGG CTCGCAGCCG AGCACGCCCA AGGAAACCAG TCCCTCGGGT 
CCCTTGAAAC GAACCTACTC GCAGTCCACC CAGAATCAAG CTGCCAGGAA GATCCAACAG 
TTCATGAGAC AATCACGCAT CAAACTACAG AGAGAGCGAG CCGAAAAAGA GAAGCTGGTG 
CACCAACGCA GGGCGGAATA CCTTCAAAAC TTGCAGTATC AAGGGCAGCA GGAAATCTTG 
GCTTGCCTAG AAAATAACAG TTCTGCCCAG AGCAGTGGCA GCAACACCTA TACGAACAGC 
ATCAACAATC TACAATCCAA TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

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Dpse\GA30460 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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