Dpse\GA12095 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA12095
Entrez Gene ID	4813545
Full Name	GA12095 gene product from transcript GA12095-RA
Synonyms	GA12095,dpse_GLEANR_12064
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001354012.3	XP_001354048.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA12095 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA12095 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe08571	XM_001354012.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA12095), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA12095 ( XM_001354012.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001354048.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA12095), mRNA.

Price & Availability↑

CloneID	ODe08571
Clone ID Related Accession (Same CDS sequence)	XM_001354012.3
Accession Version	XM_001354012.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). On Dec 22, 2015 this sequence version replaced XM_001354012.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGATC TGTCGCAGGG CGACATTTGT CGCGTGTGCC GCTGCGAGGC GCAGCAGGAC 
CGCCCGCTCT TCTATCCGTG CATCTGCACG GGCAGCATCA AGTACATCCA CCAGGACTGC 
CTGATGCAGT GGATGCGCTA TTCGCACAAG GAGTACTGCG AGCTGTGCGG CCACCGCTTC 
AGCTTTCAGC CCATCTACGC CCCGGATATG CCGCGCGTGC TGCCCGTGAA GGATGTGCTC 
GTGGGTCTCA TGTCCGCGGT GCTGGAGGCC GCCCGCTGCT GGCTCCACTA CTCGCTGGTG 
GGCCTCGCCT GGTTCGGACT GGTCCCACTC TCCGCCTACC GCACCTATCG CTATCTCTTT 
CGGGCCAACT CCTTTGACAT GATCCTCTCG CTGCCGTTCG ACATCTTCTC CATGGATAAT 
CTGCCTTCGG ATGCCTTTCG CGGATGCTTT GTGGTCACCT GTACGCTGCT CTCCTTCATT 
GGCCTCGTCT GGCTGAGGGA GCAGATCCTT CATGGCGGCG GACCCGATTG GCTGGAGCGC 
GACGATGCCC CTGCGGCCGC CCAGCCAGCT GCGGCTGCTG CCGCTCCAGG GCCTGCGGCC 
GCCCCTCCGG CCGATGAGGC AGATGCTTTC GCTGCCGCTG TGGCTGCCGT TGAGGCTGCC 
GCCCAAGATG ACAACAACAA CGGCGAGGCC CCACAGGATG TGCCCCCCAT GGACAATGCA 
GCACCGGCCC CGGCCGACAA CGAAGGCGAT GTCCAGGGAG CAGGCGGAGA TCCGAGAAAT 
CCAGCTCCAG TTGCCGCTGG GAACCCAGCA GAAGCAGAGG CCGATGAACA GAACTGGAAC 
CCCATGGAGT GGGATCGGGC CGCCGAGGAG CTGACCTGGG AGCGGCTCCT GGGACTGGAC 
GGTTCGCTGG TCTTTCTGGA GCATGTCTTC TGGATCACTT CCCTCAACAC GATGTTCATC 
TTTACGTTTG CCTTCTGCCC GTATTGCGTG GGCAACTTTA TACTGAGCTC CATGGACCTG 
CTGCAGCCGG AGAAGCCTCT GCTGCACTTC CACGGCCTGA TAACGACTCT CTTTGGCTAC 
TGCTGCATCG GACTGACGCT CGTTGTGCTG CAATTTCTGG CCCACACCTT CCAAATGCGT 
CGCGTCTGCT GGTTCATCGG CCTCTGCTAC ATTGTGGTGA AGGTCTCGCT CCTCTCTGTG 
GTGGAGATTG GCGTGCTGCC GCTCGTCTGC GGCTGGTGGC TGGACATCTG CTCGCTGCCC 
CTGCTCGATG CCAGTCTTAA GGATCGCAAG GCGAGCTTCA AGGCGGCCCC GGGCACCTCC 
CTCTTCATTC ACTGGATGTT TGGCATGGTC TATGTCTACT ACTTTGCCGC GTTCATCTCT 
CTGCTGCGAG AGGTGCTGCG TCCGGGCGTG CTATGGATCT TTCGCAACGT CAACGACCCC 
GACTTCAGTC CCATTCAGGA GATGATCCAT GTGCCCATTG TGCGGCACAT CCGGCGACTG 
GTGGCCTCGG CCATGATCTT TGGCTTCGCG GTGCTCCTGA TGCTGTGGCT GCCCATACGC 
ATACTGCAGG TGGCCTGGCC CAATTTCTTG CCCTATGCCC TGAGCGGAGA TGCGGAGGTG 
AACGATTTGA GTCTGCAGCT GTTGCTCCTG CAGATTGTCT TGCCCGGGTT CTTTGAGCAA 
ACCCAAACTC GCATCTGGCT GAAGGGTGTG CTCCGCATCT GGTGCACGGC CGTGTCCTGG 
CTTCTGGGAA TACGCAGCTA CCTATTGCCC GCTCCAGAGC CCGAGCCGGC AGCCGCCGCC 
GAAGGCGAGG GCGAGGGCGT GGGCGTGGAC GGAGCCGTTG AAAACGCCAA TCCAGATGTG 
GCGCCGCAGG CACCACCGCC ACCACCGCCG CCGCCCCCTG TGGATCCGTT TCCCATACAT 
CTACCGCGGA ATCTGGCCGC CGCCCATCAG GCGATCATGC AGCGGGATGC GCCCATGGGC 
TTCCAGCCGT ACGAGAAGCC CACTCTCTTC GCCTTCCGCC TGTGCGCGCT TATGACTCTG 
ATGTGCCTGT CGATTGTGGG CGCCGCCATA CTGACGCTGA CGGTGCCCGT GTACATTGGT 
CGCCGCCTGA TGGTGCTCTG GACGGGACAG CAGGCCGAGA AAGGAGTTGC TGCTGCTGCC 
GCGGCGGAAG GAGGACCCCT GCCCGCCGTA GATCCCGAGG CGGCAAGGAA GAATGAGCGA 
CTGTTGCGCG CCCACGAGCT GTACACCGCC GAGATTGGGG GCTATCTTTG CTGGATTGTG 
CTCCGCGGAG TGGCTGTGGT CGCGACCCTC CTGCCCCAAG GTCGCACGGC GATTGTCAAC 
AAGCTGAAGC ACTGGGCCAC CGTCACCCTG CAGTACGCCC TGCCCGTGAT CACGCTGGTG 
GGCATCTTTG TGCTTGTGCC GCTGCTTTTC GGCCTGCTCC TGGAGCTGGT GGTGGTGATA 
CCGCTGCGCG TGCCCCTACG CAAGACACCC ATACACTTCT TGTGGCAGGA CTGGGCGCTG 
GGCATGCTCT ACACAAAGAT AGCCATTGCC TTGACCCTCA TGGGGCCCGA GTGGCATCTG 
CGGCGTGCCC TGGAGCGCGC CTACACGGAT GGACTGCGAG ATTTCGACCT GAAGTTCGTG 
ATGCGGGACT TGGCCGGGCC GGTGGTGACG ACCTTTGGCC TGGCCCTGGC CATACCCTAT 
GTGATGGCCA GGATGGTGCT GCCCGTCTTC TTTGTGGATC GCTACACGCG GCAGTGCATC 
TATCGGCTGG TGTATCCCGT CAGCTTCGCC GCGGTGGGCA CCATCTGTTT CGTTCTCTTC 
CAGATCAAGC AGCTGAAGAA GCTCTATCTC TCGATCAAGG TGGACAAGTA CTTGGTGGGG 
CAGCGTCTGG TCAACTATGA GAACCGCAAG AAGCAGCAAC AGCAGCAGCA GGAGGAGGAG 
CAGAAGGCAC GCGAGCTGAA GGAGCGCAGG GACAAGGAGA AGGAGCTGGG CGTCGATATG 
GCAATGGTGC CCGAGGAGTA TGCCGATCTT TACGGCGCCA TCGACGAGGA GCCGGATCAT 
GCGGACCTCC TTGCACGTGT GAGGAGACGC CGAGAGCTAC CGCCACGGCC CCCCCTGCGG 
CCAGACGCCA ACGAAGAGCT CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001354048.2
CDS	411..3557
Translation	MDDLSQGDICRVCRCEAQQDRPLFYPCICTGSIKYIHQDCLMQWMRYSHKEYCELCGHRFSFQPIYAPDMPRVLPVKDVLVGLMSAVLEAARCWLHYSLVGLAWFGLVPLSAYRTYRYLFRANSFDMILSLPFDIFSMDNLPSDAFRGCFVVTCTLLSFIGLVWLREQILHGGGPDWLERDDAPAAAQPAAAAAAPGPAAAPPADEADAFAAAVAAVEAAAQDDNNNGEAPQDVPPMDNAAPAPADNEGDVQGAGGDPRNPAPVAAGNPAEAEADEQNWNPMEWDRAAEELTWERLLGLDGSLVFLEHVFWITSLNTMFIFTFAFCPYCVGNFILSSMDLLQPEKPLLHFHGLITTLFGYCCIGLTLVVLQFLAHTFQMRRVCWFIGLCYIVVKVSLLSVVEIGVLPLVCGWWLDICSLPLLDASLKDRKASFKAAPGTSLFIHWMFGMVYVYYFAAFISLLREVLRPGVLWIFRNVNDPDFSPIQEMIHVPIVRHIRRLVASAMIFGFAVLLMLWLPIRILQVAWPNFLPYALSGDAEVNDLSLQLLLLQIVLPGFFEQTQTRIWLKGVLRIWCTAVSWLLGIRSYLLPAPEPEPAAAAEGEGEGVGVDGAVENANPDVAPQAPPPPPPPPPVDPFPIHLPRNLAAAHQAIMQRDAPMGFQPYEKPTLFAFRLCALMTLMCLSIVGAAILTLTVPVYIGRRLMVLWTGQQAEKGVAAAAAAEGGPLPAVDPEAARKNERLLRAHELYTAEIGGYLCWIVLRGVAVVATLLPQGRTAIVNKLKHWATVTLQYALPVITLVGIFVLVPLLFGLLLELVVVIPLRVPLRKTPIHFLWQDWALGMLYTKIAIALTLMGPEWHLRRALERAYTDGLRDFDLKFVMRDLAGPVVTTFGLALAIPYVMARMVLPVFFVDRYTRQCIYRLVYPVSFAAVGTICFVLFQIKQLKKLYLSIKVDKYLVGQRLVNYENRKKQQQQQQEEEQKARELKERRDKEKELGVDMAMVPEEYADLYGAIDEEPDHADLLARVRRRRELPPRPPLRPDANEELL

Target ORF information:

RefSeq Version	XM_001354012.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA12095), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001354012.3

ORF Insert Sequence:

ATGGACGATC TGTCGCAGGG CGACATTTGT CGCGTGTGCC GCTGCGAGGC GCAGCAGGAC 
CGCCCGCTCT TCTATCCGTG CATCTGCACG GGCAGCATCA AGTACATCCA CCAGGACTGC 
CTGATGCAGT GGATGCGCTA TTCGCACAAG GAGTACTGCG AGCTGTGCGG CCACCGCTTC 
AGCTTTCAGC CCATCTACGC CCCGGATATG CCGCGCGTGC TGCCCGTGAA GGATGTGCTC 
GTGGGTCTCA TGTCCGCGGT GCTGGAGGCC GCCCGCTGCT GGCTCCACTA CTCGCTGGTG 
GGCCTCGCCT GGTTCGGACT GGTCCCACTC TCCGCCTACC GCACCTATCG CTATCTCTTT 
CGGGCCAACT CCTTTGACAT GATCCTCTCG CTGCCGTTCG ACATCTTCTC CATGGATAAT 
CTGCCTTCGG ATGCCTTTCG CGGATGCTTT GTGGTCACCT GTACGCTGCT CTCCTTCATT 
GGCCTCGTCT GGCTGAGGGA GCAGATCCTT CATGGCGGCG GACCCGATTG GCTGGAGCGC 
GACGATGCCC CTGCGGCCGC CCAGCCAGCT GCGGCTGCTG CCGCTCCAGG GCCTGCGGCC 
GCCCCTCCGG CCGATGAGGC AGATGCTTTC GCTGCCGCTG TGGCTGCCGT TGAGGCTGCC 
GCCCAAGATG ACAACAACAA CGGCGAGGCC CCACAGGATG TGCCCCCCAT GGACAATGCA 
GCACCGGCCC CGGCCGACAA CGAAGGCGAT GTCCAGGGAG CAGGCGGAGA TCCGAGAAAT 
CCAGCTCCAG TTGCCGCTGG GAACCCAGCA GAAGCAGAGG CCGATGAACA GAACTGGAAC 
CCCATGGAGT GGGATCGGGC CGCCGAGGAG CTGACCTGGG AGCGGCTCCT GGGACTGGAC 
GGTTCGCTGG TCTTTCTGGA GCATGTCTTC TGGATCACTT CCCTCAACAC GATGTTCATC 
TTTACGTTTG CCTTCTGCCC GTATTGCGTG GGCAACTTTA TACTGAGCTC CATGGACCTG 
CTGCAGCCGG AGAAGCCTCT GCTGCACTTC CACGGCCTGA TAACGACTCT CTTTGGCTAC 
TGCTGCATCG GACTGACGCT CGTTGTGCTG CAATTTCTGG CCCACACCTT CCAAATGCGT 
CGCGTCTGCT GGTTCATCGG CCTCTGCTAC ATTGTGGTGA AGGTCTCGCT CCTCTCTGTG 
GTGGAGATTG GCGTGCTGCC GCTCGTCTGC GGCTGGTGGC TGGACATCTG CTCGCTGCCC 
CTGCTCGATG CCAGTCTTAA GGATCGCAAG GCGAGCTTCA AGGCGGCCCC GGGCACCTCC 
CTCTTCATTC ACTGGATGTT TGGCATGGTC TATGTCTACT ACTTTGCCGC GTTCATCTCT 
CTGCTGCGAG AGGTGCTGCG TCCGGGCGTG CTATGGATCT TTCGCAACGT CAACGACCCC 
GACTTCAGTC CCATTCAGGA GATGATCCAT GTGCCCATTG TGCGGCACAT CCGGCGACTG 
GTGGCCTCGG CCATGATCTT TGGCTTCGCG GTGCTCCTGA TGCTGTGGCT GCCCATACGC 
ATACTGCAGG TGGCCTGGCC CAATTTCTTG CCCTATGCCC TGAGCGGAGA TGCGGAGGTG 
AACGATTTGA GTCTGCAGCT GTTGCTCCTG CAGATTGTCT TGCCCGGGTT CTTTGAGCAA 
ACCCAAACTC GCATCTGGCT GAAGGGTGTG CTCCGCATCT GGTGCACGGC CGTGTCCTGG 
CTTCTGGGAA TACGCAGCTA CCTATTGCCC GCTCCAGAGC CCGAGCCGGC AGCCGCCGCC 
GAAGGCGAGG GCGAGGGCGT GGGCGTGGAC GGAGCCGTTG AAAACGCCAA TCCAGATGTG 
GCGCCGCAGG CACCACCGCC ACCACCGCCG CCGCCCCCTG TGGATCCGTT TCCCATACAT 
CTACCGCGGA ATCTGGCCGC CGCCCATCAG GCGATCATGC AGCGGGATGC GCCCATGGGC 
TTCCAGCCGT ACGAGAAGCC CACTCTCTTC GCCTTCCGCC TGTGCGCGCT TATGACTCTG 
ATGTGCCTGT CGATTGTGGG CGCCGCCATA CTGACGCTGA CGGTGCCCGT GTACATTGGT 
CGCCGCCTGA TGGTGCTCTG GACGGGACAG CAGGCCGAGA AAGGAGTTGC TGCTGCTGCC 
GCGGCGGAAG GAGGACCCCT GCCCGCCGTA GATCCCGAGG CGGCAAGGAA GAATGAGCGA 
CTGTTGCGCG CCCACGAGCT GTACACCGCC GAGATTGGGG GCTATCTTTG CTGGATTGTG 
CTCCGCGGAG TGGCTGTGGT CGCGACCCTC CTGCCCCAAG GTCGCACGGC GATTGTCAAC 
AAGCTGAAGC ACTGGGCCAC CGTCACCCTG CAGTACGCCC TGCCCGTGAT CACGCTGGTG 
GGCATCTTTG TGCTTGTGCC GCTGCTTTTC GGCCTGCTCC TGGAGCTGGT GGTGGTGATA 
CCGCTGCGCG TGCCCCTACG CAAGACACCC ATACACTTCT TGTGGCAGGA CTGGGCGCTG 
GGCATGCTCT ACACAAAGAT AGCCATTGCC TTGACCCTCA TGGGGCCCGA GTGGCATCTG 
CGGCGTGCCC TGGAGCGCGC CTACACGGAT GGACTGCGAG ATTTCGACCT GAAGTTCGTG 
ATGCGGGACT TGGCCGGGCC GGTGGTGACG ACCTTTGGCC TGGCCCTGGC CATACCCTAT 
GTGATGGCCA GGATGGTGCT GCCCGTCTTC TTTGTGGATC GCTACACGCG GCAGTGCATC 
TATCGGCTGG TGTATCCCGT CAGCTTCGCC GCGGTGGGCA CCATCTGTTT CGTTCTCTTC 
CAGATCAAGC AGCTGAAGAA GCTCTATCTC TCGATCAAGG TGGACAAGTA CTTGGTGGGG 
CAGCGTCTGG TCAACTATGA GAACCGCAAG AAGCAGCAAC AGCAGCAGCA GGAGGAGGAG 
CAGAAGGCAC GCGAGCTGAA GGAGCGCAGG GACAAGGAGA AGGAGCTGGG CGTCGATATG 
GCAATGGTGC CCGAGGAGTA TGCCGATCTT TACGGCGCCA TCGACGAGGA GCCGGATCAT 
GCGGACCTCC TTGCACGTGT GAGGAGACGC CGAGAGCTAC CGCCACGGCC CCCCCTGCGG 
CCAGACGCCA ACGAAGAGCT CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA12095 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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