Dpse\GA21822 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA21822
Entrez Gene ID	4803778
Full Name	GA21822 gene product from transcript GA21822-RC
Synonyms	GA21822,dpse_GLEANR_15280
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015183871.1	XP_015039357.1	uncharacterized protein, isoform C
XM_001360403.3	XP_001360440.3	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA21822 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA21822 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe21584	XM_001360403.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA21822), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODe22525	XM_015183871.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA21822), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA21822 ( XM_001360403.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001360440.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA21822), mRNA.

Price & Availability↑

CloneID	ODe21584
Clone ID Related Accession (Same CDS sequence)	XM_001360403.3
Accession Version	XM_001360403.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_001360403.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAATG CAGGCAGTGC TTCTATTCGT GAGCCCGTAT CAAATCGAAC CACCCGCCAC 
AGTGAGATGA GTACCATGGG CGAAGATACG GAATCGCACA GCATACCCAT AAGTGAAGGA 
CAGAACGCTG AGCATGTGCT GTACCGCCTG AAGCGGGGCA CCCGGCTGGA CGTGCACCCG 
GACGCCTCGT TGCTGGGAAA GAAGATCGTC CTGTACACTA ACTATCCCGC CGAGGGCCAG 
AAGTTCGTGC GCACCGACTA CCGTGTCCTG GGATGGCAGC TGAAAAATGG CAAACAGATC 
ACGACTGTCA TGCATCCGGA GGCTCATGTG GTGGACACGG ATATCCACAG TCAGCTGCAG 
CTGAAACTGT CCGGAACGTA TCACTTCTAC TTCCGCTATG TTGAGCGAGC AGACACCGGA 
AGTGCAGGAG CCGATGGGGC TCTCTATGTC CAGGTGGAGC CCACCCTTCA TGTGGGGCCG 
CCGGGGGCAC AAAAGACAAT ACCTCTGAAT TCGGTGCGCT GCCAGACGGT GCTAACCAAG 
CTACTGGGAC CACTCAGCAG CTGGGAGCCA AAGTTGCGCG TGGCCAAGGA GGCCGGCTAC 
AATGTGATTC ACTTCACACC CATCCAGGAG TTGGGCGGCT CGCGCTCCGC CTACTCGCTG 
CGGGATCAGC TGAAAGTCAA CCCAAGCTTC GCAAGCAAGA AGGGCGACAA GGTTACCTTC 
CAGGACGTGG AGAAGATCAT CAAGAAGTGT CGCCAGGAGT GGGGCGTGGC CTCCATCTGC 
GACATTGTGC TGAATCACAC GGCCAACGAG TCGGAGTGGC TGCTCCAGCA TCCGGATGCC 
ACCTACTCGT GCGCCTCGTG TCCGTACCTG CGGCCAGCCT TCCTGCTGGA CGCCGCCCTG 
GCCCAGTGCG GAGCCGACAT CGCTGCCGGC AGCCTGGAGC ATGTGGGCGT GCCTCTGGTT 
ATCGATCAGG AGTGCCATTT GGAAGCTTTA AAGTACCAGT TGCATAACGC CTACCTATCC 
AAGGTCAATA TACACGAGCT GTATCAGTGC GACGTGATGC AGTATGTGAA CAACTTCATG 
AACCAGGTGC GTACTCGCGA GCCGCCCAAG AACGTGGCCA ACGAGTCGCG TTTCCAGGAG 
ATCCAGCTGA TACAGGACCC GGCATATCGA CGCTTGGGTA GCACCATCAA TTTCGAGCTG 
GCGCTGGAGA TCTTTAATGC TTTCCATGGC GATTGTTTCG ATGAGGAGTC ACGCTTCCGC 
AAGTGCGCCG AGACCCTGCG CCACCACCTG GACGGCCTCA ACGATCGCGT GCGGGCCGAG 
ATCCAAGGCT ATCTGAACTA CGCCGTCGAC AATTGCCTGG CCGGCATCCG ATACGAGCGA 
GTTCAGGGCG ACGGTCCCAG AGTGCGCGAA ATATCAGAAA AGCACTCTGT CTTTATGACC 
TACTTCACCC ACACGGGCAC CCTGGGCAAG ACGCTGCCCG AGATCGAGGC CGATATGTAC 
ACCAAGCCGG GCGAATTCTT TATGGCCCAC AACGGCTGGG TCATGGGCTC TAGCGATCCA 
TTGCTTGACT TTGCCGAGGA GCAGCCGGGA CGTGCCAATG TCTACCTCAA GCGAGAGCTC 
ATTGCCTGGG GCGACAGCGT GAAGCTCCGT TTTGGCAAGC GGCCAGAGGA TAGCCCCTAC 
CTGTGGAAGC ACATGACTGA GTATGTGGAG ACGACGGCCC GCATATTCGA TGGAGTCCGG 
CTGGACAACT GCCACTCGAC GCCGCTGCAC GTGGCTGAGT ACCTGCTGGA CGCGGCGCGG 
AAGATCAACC CCGAACTGTA TGTGGTGGCC GAGCTGTTCA CCAACTCCGA TGGCACCGAC 
AATGTGTTCG TCAATCGCCT GGGCATCACA TCCCTAATTC GCGAAGCCCT TTCGGCCTGG 
GACTCGCACG AGCAGGGCCG TCTGGTCTAT CGGTATGGCG GTGCGCCAGT CGGTGGCTTC 
TATCCGAATC CCACGCGTCA TGAGGCGTCC AGCGTTGCAC ACGCCCTGTT CATGGACCTC 
ACCCACGACA ATCCATCGGC CGTGGAAAAA CGGTCCGTCT ACGATCTGCT GCCCTCGTCT 
GCTCTGGTCT CCATGGCCTG CTGTGCCACC GGCAGCAATC GTGGCTACGA CGAACTGGTT 
CCACATCATA TCCATGTCGT CGACGAACAG CGTTTATACC AGGAATGGGG CAAGGGCGTG 
GACTCCAAGT CCGGCATTAT GGGAGCTAAG CGAGCGCTGA ATCTGCTGCA CGGACAGCTG 
GCCGAAGAGG GATTTACTCA GGTGTATGTG GACCAAATGG ACCCCAATGT GGTGGCTGTT 
ACCCGGCATT CCCCGATCAC CCATCAGTCG GTCATTCTGG TGGCTCACAC GGCCTTCGGG 
TATCCGAATC CGAATGCCGG ACCTACTGGC ATCGGACCGT TGCGCTTTGA CGGCGTTTTG 
GACGAGATCA TCCTGGAGGC CAGCCTGACC ATGAAGAGCG ACAAGCCCTT CGATCGTCCC 
GCTCCCTTTA AGAAGGATCC CAATGTGTTG AACGGCTTCA CCCAGTTCGA ACTGAGCCTG 
CAGGAGCACA TCCCGCTCAG CAAGTCGACC GTCTTTCAGA CCCATGCCAA TGTCGATGGC 
AACAACACGG TGCTGAACTT TGTTAACCTT CGGCCTGGCA CAGTGGTAGC CATTCGAGTG 
TCCATGCATC CCGGACCCCG CATCAGCTTC GACAGGCTGC AGAAGCTGAC AAATGCCTTG 
CGCCTGGGAT CGGGCGCTGA ATGGTCAGAG CTGCAGGGGA TCGTATCCAA ACTGGATTTG 
GTGGCGCTGA GTGCCGCCCT CTTTCAATGT GACGACGAGG AACGTGCCCA AGGCACAGGT 
GGCTCCGCCT ACGATATACC CAACTTTGGG CGCATTGTCT ACTGTGGCCT CCAGGGCTTT 
GTTTCACTGC TGACGGAGAT ATCGCCACAC AATGATCTGG GCCACCCGCT GTGCACTAAT 
TTACGCGACG GCAACTGGAT GATGGATTAC ATGGCCGATC GCCTGACCAA CTTCGAGGAT 
TTAAAGCCGC TCTCCGTCTG GCTGAAGGCC GCCTTCGAGC CTCTGAAGAA TATACCACGC 
TACCTCATTC CCTGCTATTT CGATGCCATT GTCAGCGGGG TCTACAACGT GCTCCTTAAC 
CAGGTCAACG AACTGATGCC AGATTTCATT AAGAACGGCC ACAGTTTCAC TCAGTCCCTG 
GCCCTCTCCA CGTTGCAGTT CCTTTCCGTC TGCAAGTCCG CCAATCTGCC AGGCTTCAGT 
CATGCCATCA ATCCACCAAA GCCACCGAAA CAATGTGTTA CACTCTCTGC TGGACTGCCG 
CATTTCTCCA CTGGTTACAT GCGCTGCTGG GGCCGTGATA CCTTCATCGC CCTGCGTGGA 
TCGATGTTCC TGACTGGGCG TTTCAACGAA GCTCGATACA TCATTATTGG ATTCGGCCAG 
ACCCTGAGAC ATGGTCTCAT ACCCAATCTC TTGGACAGCG GGAGCAAGCC GCGCTACAAC 
TGCCGCGATG CCGTCTGGTG GTGGATGTAC TCCATCAGGC AGTACGTGGA GGAGGCTCCC 
AAGGGAAGCG AGATACTGAA GGACAAGGTG TCGCGCCTCT TTCCCTACGA CGATGCCGAT 
GCACATGCAC CAGGGGCCTT CGATCAGCTG CTCATCGATG TGATGCAGGA GGCGCTGCAG 
GTGCATTTCC AGGGACTGCA ATATCGCGAA CGCAACGCGG GCCGTGAGAT AGATGAGCAT 
ATGGTGGACC AGGGATTCAA CAATCACATT GGAGTGCATC CGGAAACTGG CTTTGTGTTT 
GGCGGAAACA ACTTTAATGC CGGCACGTGG ATGGACAAGA TGGGCTCGTC GCAGAAGGCC 
GGCAACAAGG GGCGGCCGAG CACCCCACGC GATGGATCCG CTGTGGAGTT GATTGGCCTC 
CAATATTCTG TATTGCGCTT CATGCAGGGC CTGGCCGAAA AGGAGGCCAT CCCGTACACG 
GGTGTGGATC GCAAGGGGCC GTCAGGTGAG TTGACAAAGT GGAGCTACAA GGAGTGGGCA 
GATCGCATCA AGGACAACTT CGATCGCCAT TTCTTTGTCC CGGAGTCAGA GACATGTTCC 
TTGGCAAACA AGAGGTTCAT CTACAAGGAC AGCTACGGCG CAACCCAGAG CTGGACAGAC 
TATCAGCTCC GTTGTAACTT CCCCATCACA CTGACCGTCG CTTCGGAGCT TTGCAATCCG 
CAGAACGCCT GGCGTGCTCT GGAGCTGGCC AAGCAGTACC TGCTTGGACC ACTGGGCATG 
AAGACACTGG ATCCCGAGGA CTGGAACTAC AGGGCTAACT ATGATAACTC CAACGACTCG 
ACGGATTGCA CCGTTGCCCA TGGATCCAAC TACCACCAGG GACCCGAGTG GGTATGGCCC 
ATTGGCTTCT ACCTGCGGGC TCGTCTCATC TTCGCCAAGA AATGCGGCCA TCTAGATGAA 
ACCATTGCTG AGACCTGGGC CATTCTGCGG GCCCATCTCC GTGAGATGCA GACCTCCCAC 
TGGCGTGGCC TGCCCGAGCT GACCAACGAC AATGGCTCCT ACTGCGGCGA CTCCTGCCGC 
ACCCAGGCCT GGAGCGTGGC CGCCATTCTC GAGGTTCTCT ACGATCTGCA CGCCCTAGGA 
GCGGACGTAG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001360440.3
CDS	93..4787
Translation	MGNAGSASIREPVSNRTTRHSEMSTMGEDTESHSIPISEGQNAEHVLYRLKRGTRLDVHPDASLLGKKIVLYTNYPAEGQKFVRTDYRVLGWQLKNGKQITTVMHPEAHVVDTDIHSQLQLKLSGTYHFYFRYVERADTGSAGADGALYVQVEPTLHVGPPGAQKTIPLNSVRCQTVLTKLLGPLSSWEPKLRVAKEAGYNVIHFTPIQELGGSRSAYSLRDQLKVNPSFASKKGDKVTFQDVEKIIKKCRQEWGVASICDIVLNHTANESEWLLQHPDATYSCASCPYLRPAFLLDAALAQCGADIAAGSLEHVGVPLVIDQECHLEALKYQLHNAYLSKVNIHELYQCDVMQYVNNFMNQVRTREPPKNVANESRFQEIQLIQDPAYRRLGSTINFELALEIFNAFHGDCFDEESRFRKCAETLRHHLDGLNDRVRAEIQGYLNYAVDNCLAGIRYERVQGDGPRVREISEKHSVFMTYFTHTGTLGKTLPEIEADMYTKPGEFFMAHNGWVMGSSDPLLDFAEEQPGRANVYLKRELIAWGDSVKLRFGKRPEDSPYLWKHMTEYVETTARIFDGVRLDNCHSTPLHVAEYLLDAARKINPELYVVAELFTNSDGTDNVFVNRLGITSLIREALSAWDSHEQGRLVYRYGGAPVGGFYPNPTRHEASSVAHALFMDLTHDNPSAVEKRSVYDLLPSSALVSMACCATGSNRGYDELVPHHIHVVDEQRLYQEWGKGVDSKSGIMGAKRALNLLHGQLAEEGFTQVYVDQMDPNVVAVTRHSPITHQSVILVAHTAFGYPNPNAGPTGIGPLRFDGVLDEIILEASLTMKSDKPFDRPAPFKKDPNVLNGFTQFELSLQEHIPLSKSTVFQTHANVDGNNTVLNFVNLRPGTVVAIRVSMHPGPRISFDRLQKLTNALRLGSGAEWSELQGIVSKLDLVALSAALFQCDDEERAQGTGGSAYDIPNFGRIVYCGLQGFVSLLTEISPHNDLGHPLCTNLRDGNWMMDYMADRLTNFEDLKPLSVWLKAAFEPLKNIPRYLIPCYFDAIVSGVYNVLLNQVNELMPDFIKNGHSFTQSLALSTLQFLSVCKSANLPGFSHAINPPKPPKQCVTLSAGLPHFSTGYMRCWGRDTFIALRGSMFLTGRFNEARYIIIGFGQTLRHGLIPNLLDSGSKPRYNCRDAVWWWMYSIRQYVEEAPKGSEILKDKVSRLFPYDDADAHAPGAFDQLLIDVMQEALQVHFQGLQYRERNAGREIDEHMVDQGFNNHIGVHPETGFVFGGNNFNAGTWMDKMGSSQKAGNKGRPSTPRDGSAVELIGLQYSVLRFMQGLAEKEAIPYTGVDRKGPSGELTKWSYKEWADRIKDNFDRHFFVPESETCSLANKRFIYKDSYGATQSWTDYQLRCNFPITLTVASELCNPQNAWRALELAKQYLLGPLGMKTLDPEDWNYRANYDNSNDSTDCTVAHGSNYHQGPEWVWPIGFYLRARLIFAKKCGHLDETIAETWAILRAHLREMQTSHWRGLPELTNDNGSYCGDSCRTQAWSVAAILEVLYDLHALGADVA

Target ORF information:

RefSeq Version	XM_001360403.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA21822), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001360403.3

ORF Insert Sequence:

ATGGGAAATG CAGGCAGTGC TTCTATTCGT GAGCCCGTAT CAAATCGAAC CACCCGCCAC 
AGTGAGATGA GTACCATGGG CGAAGATACG GAATCGCACA GCATACCCAT AAGTGAAGGA 
CAGAACGCTG AGCATGTGCT GTACCGCCTG AAGCGGGGCA CCCGGCTGGA CGTGCACCCG 
GACGCCTCGT TGCTGGGAAA GAAGATCGTC CTGTACACTA ACTATCCCGC CGAGGGCCAG 
AAGTTCGTGC GCACCGACTA CCGTGTCCTG GGATGGCAGC TGAAAAATGG CAAACAGATC 
ACGACTGTCA TGCATCCGGA GGCTCATGTG GTGGACACGG ATATCCACAG TCAGCTGCAG 
CTGAAACTGT CCGGAACGTA TCACTTCTAC TTCCGCTATG TTGAGCGAGC AGACACCGGA 
AGTGCAGGAG CCGATGGGGC TCTCTATGTC CAGGTGGAGC CCACCCTTCA TGTGGGGCCG 
CCGGGGGCAC AAAAGACAAT ACCTCTGAAT TCGGTGCGCT GCCAGACGGT GCTAACCAAG 
CTACTGGGAC CACTCAGCAG CTGGGAGCCA AAGTTGCGCG TGGCCAAGGA GGCCGGCTAC 
AATGTGATTC ACTTCACACC CATCCAGGAG TTGGGCGGCT CGCGCTCCGC CTACTCGCTG 
CGGGATCAGC TGAAAGTCAA CCCAAGCTTC GCAAGCAAGA AGGGCGACAA GGTTACCTTC 
CAGGACGTGG AGAAGATCAT CAAGAAGTGT CGCCAGGAGT GGGGCGTGGC CTCCATCTGC 
GACATTGTGC TGAATCACAC GGCCAACGAG TCGGAGTGGC TGCTCCAGCA TCCGGATGCC 
ACCTACTCGT GCGCCTCGTG TCCGTACCTG CGGCCAGCCT TCCTGCTGGA CGCCGCCCTG 
GCCCAGTGCG GAGCCGACAT CGCTGCCGGC AGCCTGGAGC ATGTGGGCGT GCCTCTGGTT 
ATCGATCAGG AGTGCCATTT GGAAGCTTTA AAGTACCAGT TGCATAACGC CTACCTATCC 
AAGGTCAATA TACACGAGCT GTATCAGTGC GACGTGATGC AGTATGTGAA CAACTTCATG 
AACCAGGTGC GTACTCGCGA GCCGCCCAAG AACGTGGCCA ACGAGTCGCG TTTCCAGGAG 
ATCCAGCTGA TACAGGACCC GGCATATCGA CGCTTGGGTA GCACCATCAA TTTCGAGCTG 
GCGCTGGAGA TCTTTAATGC TTTCCATGGC GATTGTTTCG ATGAGGAGTC ACGCTTCCGC 
AAGTGCGCCG AGACCCTGCG CCACCACCTG GACGGCCTCA ACGATCGCGT GCGGGCCGAG 
ATCCAAGGCT ATCTGAACTA CGCCGTCGAC AATTGCCTGG CCGGCATCCG ATACGAGCGA 
GTTCAGGGCG ACGGTCCCAG AGTGCGCGAA ATATCAGAAA AGCACTCTGT CTTTATGACC 
TACTTCACCC ACACGGGCAC CCTGGGCAAG ACGCTGCCCG AGATCGAGGC CGATATGTAC 
ACCAAGCCGG GCGAATTCTT TATGGCCCAC AACGGCTGGG TCATGGGCTC TAGCGATCCA 
TTGCTTGACT TTGCCGAGGA GCAGCCGGGA CGTGCCAATG TCTACCTCAA GCGAGAGCTC 
ATTGCCTGGG GCGACAGCGT GAAGCTCCGT TTTGGCAAGC GGCCAGAGGA TAGCCCCTAC 
CTGTGGAAGC ACATGACTGA GTATGTGGAG ACGACGGCCC GCATATTCGA TGGAGTCCGG 
CTGGACAACT GCCACTCGAC GCCGCTGCAC GTGGCTGAGT ACCTGCTGGA CGCGGCGCGG 
AAGATCAACC CCGAACTGTA TGTGGTGGCC GAGCTGTTCA CCAACTCCGA TGGCACCGAC 
AATGTGTTCG TCAATCGCCT GGGCATCACA TCCCTAATTC GCGAAGCCCT TTCGGCCTGG 
GACTCGCACG AGCAGGGCCG TCTGGTCTAT CGGTATGGCG GTGCGCCAGT CGGTGGCTTC 
TATCCGAATC CCACGCGTCA TGAGGCGTCC AGCGTTGCAC ACGCCCTGTT CATGGACCTC 
ACCCACGACA ATCCATCGGC CGTGGAAAAA CGGTCCGTCT ACGATCTGCT GCCCTCGTCT 
GCTCTGGTCT CCATGGCCTG CTGTGCCACC GGCAGCAATC GTGGCTACGA CGAACTGGTT 
CCACATCATA TCCATGTCGT CGACGAACAG CGTTTATACC AGGAATGGGG CAAGGGCGTG 
GACTCCAAGT CCGGCATTAT GGGAGCTAAG CGAGCGCTGA ATCTGCTGCA CGGACAGCTG 
GCCGAAGAGG GATTTACTCA GGTGTATGTG GACCAAATGG ACCCCAATGT GGTGGCTGTT 
ACCCGGCATT CCCCGATCAC CCATCAGTCG GTCATTCTGG TGGCTCACAC GGCCTTCGGG 
TATCCGAATC CGAATGCCGG ACCTACTGGC ATCGGACCGT TGCGCTTTGA CGGCGTTTTG 
GACGAGATCA TCCTGGAGGC CAGCCTGACC ATGAAGAGCG ACAAGCCCTT CGATCGTCCC 
GCTCCCTTTA AGAAGGATCC CAATGTGTTG AACGGCTTCA CCCAGTTCGA ACTGAGCCTG 
CAGGAGCACA TCCCGCTCAG CAAGTCGACC GTCTTTCAGA CCCATGCCAA TGTCGATGGC 
AACAACACGG TGCTGAACTT TGTTAACCTT CGGCCTGGCA CAGTGGTAGC CATTCGAGTG 
TCCATGCATC CCGGACCCCG CATCAGCTTC GACAGGCTGC AGAAGCTGAC AAATGCCTTG 
CGCCTGGGAT CGGGCGCTGA ATGGTCAGAG CTGCAGGGGA TCGTATCCAA ACTGGATTTG 
GTGGCGCTGA GTGCCGCCCT CTTTCAATGT GACGACGAGG AACGTGCCCA AGGCACAGGT 
GGCTCCGCCT ACGATATACC CAACTTTGGG CGCATTGTCT ACTGTGGCCT CCAGGGCTTT 
GTTTCACTGC TGACGGAGAT ATCGCCACAC AATGATCTGG GCCACCCGCT GTGCACTAAT 
TTACGCGACG GCAACTGGAT GATGGATTAC ATGGCCGATC GCCTGACCAA CTTCGAGGAT 
TTAAAGCCGC TCTCCGTCTG GCTGAAGGCC GCCTTCGAGC CTCTGAAGAA TATACCACGC 
TACCTCATTC CCTGCTATTT CGATGCCATT GTCAGCGGGG TCTACAACGT GCTCCTTAAC 
CAGGTCAACG AACTGATGCC AGATTTCATT AAGAACGGCC ACAGTTTCAC TCAGTCCCTG 
GCCCTCTCCA CGTTGCAGTT CCTTTCCGTC TGCAAGTCCG CCAATCTGCC AGGCTTCAGT 
CATGCCATCA ATCCACCAAA GCCACCGAAA CAATGTGTTA CACTCTCTGC TGGACTGCCG 
CATTTCTCCA CTGGTTACAT GCGCTGCTGG GGCCGTGATA CCTTCATCGC CCTGCGTGGA 
TCGATGTTCC TGACTGGGCG TTTCAACGAA GCTCGATACA TCATTATTGG ATTCGGCCAG 
ACCCTGAGAC ATGGTCTCAT ACCCAATCTC TTGGACAGCG GGAGCAAGCC GCGCTACAAC 
TGCCGCGATG CCGTCTGGTG GTGGATGTAC TCCATCAGGC AGTACGTGGA GGAGGCTCCC 
AAGGGAAGCG AGATACTGAA GGACAAGGTG TCGCGCCTCT TTCCCTACGA CGATGCCGAT 
GCACATGCAC CAGGGGCCTT CGATCAGCTG CTCATCGATG TGATGCAGGA GGCGCTGCAG 
GTGCATTTCC AGGGACTGCA ATATCGCGAA CGCAACGCGG GCCGTGAGAT AGATGAGCAT 
ATGGTGGACC AGGGATTCAA CAATCACATT GGAGTGCATC CGGAAACTGG CTTTGTGTTT 
GGCGGAAACA ACTTTAATGC CGGCACGTGG ATGGACAAGA TGGGCTCGTC GCAGAAGGCC 
GGCAACAAGG GGCGGCCGAG CACCCCACGC GATGGATCCG CTGTGGAGTT GATTGGCCTC 
CAATATTCTG TATTGCGCTT CATGCAGGGC CTGGCCGAAA AGGAGGCCAT CCCGTACACG 
GGTGTGGATC GCAAGGGGCC GTCAGGTGAG TTGACAAAGT GGAGCTACAA GGAGTGGGCA 
GATCGCATCA AGGACAACTT CGATCGCCAT TTCTTTGTCC CGGAGTCAGA GACATGTTCC 
TTGGCAAACA AGAGGTTCAT CTACAAGGAC AGCTACGGCG CAACCCAGAG CTGGACAGAC 
TATCAGCTCC GTTGTAACTT CCCCATCACA CTGACCGTCG CTTCGGAGCT TTGCAATCCG 
CAGAACGCCT GGCGTGCTCT GGAGCTGGCC AAGCAGTACC TGCTTGGACC ACTGGGCATG 
AAGACACTGG ATCCCGAGGA CTGGAACTAC AGGGCTAACT ATGATAACTC CAACGACTCG 
ACGGATTGCA CCGTTGCCCA TGGATCCAAC TACCACCAGG GACCCGAGTG GGTATGGCCC 
ATTGGCTTCT ACCTGCGGGC TCGTCTCATC TTCGCCAAGA AATGCGGCCA TCTAGATGAA 
ACCATTGCTG AGACCTGGGC CATTCTGCGG GCCCATCTCC GTGAGATGCA GACCTCCCAC 
TGGCGTGGCC TGCCCGAGCT GACCAACGAC AATGGCTCCT ACTGCGGCGA CTCCTGCCGC 
ACCCAGGCCT GGAGCGTGGC CGCCATTCTC GAGGTTCTCT ACGATCTGCA CGCCCTAGGA 
GCGGACGTAG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA21822 ( XM_015183871.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015039357.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA21822), mRNA.

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CloneID	ODe22525
Clone ID Related Accession (Same CDS sequence)	XM_015183871.1
Accession Version	XM_015183871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGTTAG AAGGGTTCTG CAGGAGCGTC CTGCGCTATG TGTACGCACT GCTCCAGTTC 
TTGATGCGCT ATCATGCCTT TCGCTGGCTC GTCGATTTAG TGGATTTGCA AGCGGTTGAT 
AAGATGAGTA CCATGGGCGA AGATACGGAA TCGCACAGCA TACCCATAAG TGAAGGACAG 
AACGCTGAGC ATGTGCTGTA CCGCCTGAAG CGGGGCACCC GGCTGGACGT GCACCCGGAC 
GCCTCGTTGC TGGGAAAGAA GATCGTCCTG TACACTAACT ATCCCGCCGA GGGCCAGAAG 
TTCGTGCGCA CCGACTACCG TGTCCTGGGA TGGCAGCTGA AAAATGGCAA ACAGATCACG 
ACTGTCATGC ATCCGGAGGC TCATGTGGTG GACACGGATA TCCACAGTCA GCTGCAGCTG 
AAACTGTCCG GAACGTATCA CTTCTACTTC CGCTATGTTG AGCGAGCAGA CACCGGAAGT 
GCAGGAGCCG ATGGGGCTCT CTATGTCCAG GTGGAGCCCA CCCTTCATGT GGGGCCGCCG 
GGGGCACAAA AGACAATACC TCTGAATTCG GTGCGCTGCC AGACGGTGCT AACCAAGCTA 
CTGGGACCAC TCAGCAGCTG GGAGCCAAAG TTGCGCGTGG CCAAGGAGGC CGGCTACAAT 
GTGATTCACT TCACACCCAT CCAGGAGTTG GGCGGCTCGC GCTCCGCCTA CTCGCTGCGG 
GATCAGCTGA AAGTCAACCC AAGCTTCGCA AGCAAGAAGG GCGACAAGGT TACCTTCCAG 
GACGTGGAGA AGATCATCAA GAAGTGTCGC CAGGAGTGGG GCGTGGCCTC CATCTGCGAC 
ATTGTGCTGA ATCACACGGC CAACGAGTCG GAGTGGCTGC TCCAGCATCC GGATGCCACC 
TACTCGTGCG CCTCGTGTCC GTACCTGCGG CCAGCCTTCC TGCTGGACGC CGCCCTGGCC 
CAGTGCGGAG CCGACATCGC TGCCGGCAGC CTGGAGCATG TGGGCGTGCC TCTGGTTATC 
GATCAGGAGT GCCATTTGGA AGCTTTAAAG TACCAGTTGC ATAACGCCTA CCTATCCAAG 
GTCAATATAC ACGAGCTGTA TCAGTGCGAC GTGATGCAGT ATGTGAACAA CTTCATGAAC 
CAGGTGCGTA CTCGCGAGCC GCCCAAGAAC GTGGCCAACG AGTCGCGTTT CCAGGAGATC 
CAGCTGATAC AGGACCCGGC ATATCGACGC TTGGGTAGCA CCATCAATTT CGAGCTGGCG 
CTGGAGATCT TTAATGCTTT CCATGGCGAT TGTTTCGATG AGGAGTCACG CTTCCGCAAG 
TGCGCCGAGA CCCTGCGCCA CCACCTGGAC GGCCTCAACG ATCGCGTGCG GGCCGAGATC 
CAAGGCTATC TGAACTACGC CGTCGACAAT TGCCTGGCCG GCATCCGATA CGAGCGAGTT 
CAGGGCGACG GTCCCAGAGT GCGCGAAATA TCAGAAAAGC ACTCTGTCTT TATGACCTAC 
TTCACCCACA CGGGCACCCT GGGCAAGACG CTGCCCGAGA TCGAGGCCGA TATGTACACC 
AAGCCGGGCG AATTCTTTAT GGCCCACAAC GGCTGGGTCA TGGGCTCTAG CGATCCATTG 
CTTGACTTTG CCGAGGAGCA GCCGGGACGT GCCAATGTCT ACCTCAAGCG AGAGCTCATT 
GCCTGGGGCG ACAGCGTGAA GCTCCGTTTT GGCAAGCGGC CAGAGGATAG CCCCTACCTG 
TGGAAGCACA TGACTGAGTA TGTGGAGACG ACGGCCCGCA TATTCGATGG AGTCCGGCTG 
GACAACTGCC ACTCGACGCC GCTGCACGTG GCTGAGTACC TGCTGGACGC GGCGCGGAAG 
ATCAACCCCG AACTGTATGT GGTGGCCGAG CTGTTCACCA ACTCCGATGG CACCGACAAT 
GTGTTCGTCA ATCGCCTGGG CATCACATCC CTAATTCGCG AAGCCCTTTC GGCCTGGGAC 
TCGCACGAGC AGGGCCGTCT GGTCTATCGG TATGGCGGTG CGCCAGTCGG TGGCTTCTAT 
CCGAATCCCA CGCGTCATGA GGCGTCCAGC GTTGCACACG CCCTGTTCAT GGACCTCACC 
CACGACAATC CATCGGCCGT GGAAAAACGG TCCGTCTACG ATCTGCTGCC CTCGTCTGCT 
CTGGTCTCCA TGGCCTGCTG TGCCACCGGC AGCAATCGTG GCTACGACGA ACTGGTTCCA 
CATCATATCC ATGTCGTCGA CGAACAGCGT TTATACCAGG AATGGGGCAA GGGCGTGGAC 
TCCAAGTCCG GCATTATGGG AGCTAAGCGA GCGCTGAATC TGCTGCACGG ACAGCTGGCC 
GAAGAGGGAT TTACTCAGGT GTATGTGGAC CAAATGGACC CCAATGTGGT GGCTGTTACC 
CGGCATTCCC CGATCACCCA TCAGTCGGTC ATTCTGGTGG CTCACACGGC CTTCGGGTAT 
CCGAATCCGA ATGCCGGACC TACTGGCATC GGACCGTTGC GCTTTGACGG CGTTTTGGAC 
GAGATCATCC TGGAGGCCAG CCTGACCATG AAGAGCGACA AGCCCTTCGA TCGTCCCGCT 
CCCTTTAAGA AGGATCCCAA TGTGTTGAAC GGCTTCACCC AGTTCGAACT GAGCCTGCAG 
GAGCACATCC CGCTCAGCAA GTCGACCGTC TTTCAGACCC ATGCCAATGT CGATGGCAAC 
AACACGGTGC TGAACTTTGT TAACCTTCGG CCTGGCACAG TGGTAGCCAT TCGAGTGTCC 
ATGCATCCCG GACCCCGCAT CAGCTTCGAC AGGCTGCAGA AGCTGACAAA TGCCTTGCGC 
CTGGGATCGG GCGCTGAATG GTCAGAGCTG CAGGGGATCG TATCCAAACT GGATTTGGTG 
GCGCTGAGTG CCGCCCTCTT TCAATGTGAC GACGAGGAAC GTGCCCAAGG CACAGGTGGC 
TCCGCCTACG ATATACCCAA CTTTGGGCGC ATTGTCTACT GTGGCCTCCA GGGCTTTGTT 
TCACTGCTGA CGGAGATATC GCCACACAAT GATCTGGGCC ACCCGCTGTG CACTAATTTA 
CGCGACGGCA ACTGGATGAT GGATTACATG GCCGATCGCC TGACCAACTT CGAGGATTTA 
AAGCCGCTCT CCGTCTGGCT GAAGGCCGCC TTCGAGCCTC TGAAGAATAT ACCACGCTAC 
CTCATTCCCT GCTATTTCGA TGCCATTGTC AGCGGGGTCT ACAACGTGCT CCTTAACCAG 
GTCAACGAAC TGATGCCAGA TTTCATTAAG AACGGCCACA GTTTCACTCA GTCCCTGGCC 
CTCTCCACGT TGCAGTTCCT TTCCGTCTGC AAGTCCGCCA ATCTGCCAGG CTTCAGTCAT 
GCCATCAATC CACCAAAGCC ACCGAAACAA TGTGTTACAC TCTCTGCTGG ACTGCCGCAT 
TTCTCCACTG GTTACATGCG CTGCTGGGGC CGTGATACCT TCATCGCCCT GCGTGGATCG 
ATGTTCCTGA CTGGGCGTTT CAACGAAGCT CGATACATCA TTATTGGATT CGGCCAGACC 
CTGAGACATG GTCTCATACC CAATCTCTTG GACAGCGGGA GCAAGCCGCG CTACAACTGC 
CGCGATGCCG TCTGGTGGTG GATGTACTCC ATCAGGCAGT ACGTGGAGGA GGCTCCCAAG 
GGAAGCGAGA TACTGAAGGA CAAGGTGTCG CGCCTCTTTC CCTACGACGA TGCCGATGCA 
CATGCACCAG GGGCCTTCGA TCAGCTGCTC ATCGATGTGA TGCAGGAGGC GCTGCAGGTG 
CATTTCCAGG GACTGCAATA TCGCGAACGC AACGCGGGCC GTGAGATAGA TGAGCATATG 
GTGGACCAGG GATTCAACAA TCACATTGGA GTGCATCCGG AAACTGGCTT TGTGTTTGGC 
GGAAACAACT TTAATGCCGG CACGTGGATG GACAAGATGG GCTCGTCGCA GAAGGCCGGC 
AACAAGGGGC GGCCGAGCAC CCCACGCGAT GGATCCGCTG TGGAGTTGAT TGGCCTCCAA 
TATTCTGTAT TGCGCTTCAT GCAGGGCCTG GCCGAAAAGG AGGCCATCCC GTACACGGGT 
GTGGATCGCA AGGGGCCGTC AGGTGAGTTG ACAAAGTGGA GCTACAAGGA GTGGGCAGAT 
CGCATCAAGG ACAACTTCGA TCGCCATTTC TTTGTCCCGG AGTCAGAGAC ATGTTCCTTG 
GCAAACAAGA GGTTCATCTA CAAGGACAGC TACGGCGCAA CCCAGAGCTG GACAGACTAT 
CAGCTCCGTT GTAACTTCCC CATCACACTG ACCGTCGCTT CGGAGCTTTG CAATCCGCAG 
AACGCCTGGC GTGCTCTGGA GCTGGCCAAG CAGTACCTGC TTGGACCACT GGGCATGAAG 
ACACTGGATC CCGAGGACTG GAACTACAGG GCTAACTATG ATAACTCCAA CGACTCGACG 
GATTGCACCG TTGCCCATGG ATCCAACTAC CACCAGGGAC CCGAGTGGGT ATGGCCCATT 
GGCTTCTACC TGCGGGCTCG TCTCATCTTC GCCAAGAAAT GCGGCCATCT AGATGAAACC 
ATTGCTGAGA CCTGGGCCAT TCTGCGGGCC CATCTCCGTG AGATGCAGAC CTCCCACTGG 
CGTGGCCTGC CCGAGCTGAC CAACGACAAT GGCTCCTACT GCGGCGACTC CTGCCGCACC 
CAGGCCTGGA GCGTGGCCGC CATTCTCGAG GTTCTCTACG ATCTGCACGC CCTAGGAGCG 
GACGTAGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015039357.1
CDS	11..4762
Translation	MVLEGFCRSVLRYVYALLQFLMRYHAFRWLVDLVDLQAVDKMSTMGEDTESHSIPISEGQNAEHVLYRLKRGTRLDVHPDASLLGKKIVLYTNYPAEGQKFVRTDYRVLGWQLKNGKQITTVMHPEAHVVDTDIHSQLQLKLSGTYHFYFRYVERADTGSAGADGALYVQVEPTLHVGPPGAQKTIPLNSVRCQTVLTKLLGPLSSWEPKLRVAKEAGYNVIHFTPIQELGGSRSAYSLRDQLKVNPSFASKKGDKVTFQDVEKIIKKCRQEWGVASICDIVLNHTANESEWLLQHPDATYSCASCPYLRPAFLLDAALAQCGADIAAGSLEHVGVPLVIDQECHLEALKYQLHNAYLSKVNIHELYQCDVMQYVNNFMNQVRTREPPKNVANESRFQEIQLIQDPAYRRLGSTINFELALEIFNAFHGDCFDEESRFRKCAETLRHHLDGLNDRVRAEIQGYLNYAVDNCLAGIRYERVQGDGPRVREISEKHSVFMTYFTHTGTLGKTLPEIEADMYTKPGEFFMAHNGWVMGSSDPLLDFAEEQPGRANVYLKRELIAWGDSVKLRFGKRPEDSPYLWKHMTEYVETTARIFDGVRLDNCHSTPLHVAEYLLDAARKINPELYVVAELFTNSDGTDNVFVNRLGITSLIREALSAWDSHEQGRLVYRYGGAPVGGFYPNPTRHEASSVAHALFMDLTHDNPSAVEKRSVYDLLPSSALVSMACCATGSNRGYDELVPHHIHVVDEQRLYQEWGKGVDSKSGIMGAKRALNLLHGQLAEEGFTQVYVDQMDPNVVAVTRHSPITHQSVILVAHTAFGYPNPNAGPTGIGPLRFDGVLDEIILEASLTMKSDKPFDRPAPFKKDPNVLNGFTQFELSLQEHIPLSKSTVFQTHANVDGNNTVLNFVNLRPGTVVAIRVSMHPGPRISFDRLQKLTNALRLGSGAEWSELQGIVSKLDLVALSAALFQCDDEERAQGTGGSAYDIPNFGRIVYCGLQGFVSLLTEISPHNDLGHPLCTNLRDGNWMMDYMADRLTNFEDLKPLSVWLKAAFEPLKNIPRYLIPCYFDAIVSGVYNVLLNQVNELMPDFIKNGHSFTQSLALSTLQFLSVCKSANLPGFSHAINPPKPPKQCVTLSAGLPHFSTGYMRCWGRDTFIALRGSMFLTGRFNEARYIIIGFGQTLRHGLIPNLLDSGSKPRYNCRDAVWWWMYSIRQYVEEAPKGSEILKDKVSRLFPYDDADAHAPGAFDQLLIDVMQEALQVHFQGLQYRERNAGREIDEHMVDQGFNNHIGVHPETGFVFGGNNFNAGTWMDKMGSSQKAGNKGRPSTPRDGSAVELIGLQYSVLRFMQGLAEKEAIPYTGVDRKGPSGELTKWSYKEWADRIKDNFDRHFFVPESETCSLANKRFIYKDSYGATQSWTDYQLRCNFPITLTVASELCNPQNAWRALELAKQYLLGPLGMKTLDPEDWNYRANYDNSNDSTDCTVAHGSNYHQGPEWVWPIGFYLRARLIFAKKCGHLDETIAETWAILRAHLREMQTSHWRGLPELTNDNGSYCGDSCRTQAWSVAAILEVLYDLHALGADVA

Target ORF information:

RefSeq Version	XM_015183871.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA21822), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015183871.1

ORF Insert Sequence:

ATGGTGTTAG AAGGGTTCTG CAGGAGCGTC CTGCGCTATG TGTACGCACT GCTCCAGTTC 
TTGATGCGCT ATCATGCCTT TCGCTGGCTC GTCGATTTAG TGGATTTGCA AGCGGTTGAT 
AAGATGAGTA CCATGGGCGA AGATACGGAA TCGCACAGCA TACCCATAAG TGAAGGACAG 
AACGCTGAGC ATGTGCTGTA CCGCCTGAAG CGGGGCACCC GGCTGGACGT GCACCCGGAC 
GCCTCGTTGC TGGGAAAGAA GATCGTCCTG TACACTAACT ATCCCGCCGA GGGCCAGAAG 
TTCGTGCGCA CCGACTACCG TGTCCTGGGA TGGCAGCTGA AAAATGGCAA ACAGATCACG 
ACTGTCATGC ATCCGGAGGC TCATGTGGTG GACACGGATA TCCACAGTCA GCTGCAGCTG 
AAACTGTCCG GAACGTATCA CTTCTACTTC CGCTATGTTG AGCGAGCAGA CACCGGAAGT 
GCAGGAGCCG ATGGGGCTCT CTATGTCCAG GTGGAGCCCA CCCTTCATGT GGGGCCGCCG 
GGGGCACAAA AGACAATACC TCTGAATTCG GTGCGCTGCC AGACGGTGCT AACCAAGCTA 
CTGGGACCAC TCAGCAGCTG GGAGCCAAAG TTGCGCGTGG CCAAGGAGGC CGGCTACAAT 
GTGATTCACT TCACACCCAT CCAGGAGTTG GGCGGCTCGC GCTCCGCCTA CTCGCTGCGG 
GATCAGCTGA AAGTCAACCC AAGCTTCGCA AGCAAGAAGG GCGACAAGGT TACCTTCCAG 
GACGTGGAGA AGATCATCAA GAAGTGTCGC CAGGAGTGGG GCGTGGCCTC CATCTGCGAC 
ATTGTGCTGA ATCACACGGC CAACGAGTCG GAGTGGCTGC TCCAGCATCC GGATGCCACC 
TACTCGTGCG CCTCGTGTCC GTACCTGCGG CCAGCCTTCC TGCTGGACGC CGCCCTGGCC 
CAGTGCGGAG CCGACATCGC TGCCGGCAGC CTGGAGCATG TGGGCGTGCC TCTGGTTATC 
GATCAGGAGT GCCATTTGGA AGCTTTAAAG TACCAGTTGC ATAACGCCTA CCTATCCAAG 
GTCAATATAC ACGAGCTGTA TCAGTGCGAC GTGATGCAGT ATGTGAACAA CTTCATGAAC 
CAGGTGCGTA CTCGCGAGCC GCCCAAGAAC GTGGCCAACG AGTCGCGTTT CCAGGAGATC 
CAGCTGATAC AGGACCCGGC ATATCGACGC TTGGGTAGCA CCATCAATTT CGAGCTGGCG 
CTGGAGATCT TTAATGCTTT CCATGGCGAT TGTTTCGATG AGGAGTCACG CTTCCGCAAG 
TGCGCCGAGA CCCTGCGCCA CCACCTGGAC GGCCTCAACG ATCGCGTGCG GGCCGAGATC 
CAAGGCTATC TGAACTACGC CGTCGACAAT TGCCTGGCCG GCATCCGATA CGAGCGAGTT 
CAGGGCGACG GTCCCAGAGT GCGCGAAATA TCAGAAAAGC ACTCTGTCTT TATGACCTAC 
TTCACCCACA CGGGCACCCT GGGCAAGACG CTGCCCGAGA TCGAGGCCGA TATGTACACC 
AAGCCGGGCG AATTCTTTAT GGCCCACAAC GGCTGGGTCA TGGGCTCTAG CGATCCATTG 
CTTGACTTTG CCGAGGAGCA GCCGGGACGT GCCAATGTCT ACCTCAAGCG AGAGCTCATT 
GCCTGGGGCG ACAGCGTGAA GCTCCGTTTT GGCAAGCGGC CAGAGGATAG CCCCTACCTG 
TGGAAGCACA TGACTGAGTA TGTGGAGACG ACGGCCCGCA TATTCGATGG AGTCCGGCTG 
GACAACTGCC ACTCGACGCC GCTGCACGTG GCTGAGTACC TGCTGGACGC GGCGCGGAAG 
ATCAACCCCG AACTGTATGT GGTGGCCGAG CTGTTCACCA ACTCCGATGG CACCGACAAT 
GTGTTCGTCA ATCGCCTGGG CATCACATCC CTAATTCGCG AAGCCCTTTC GGCCTGGGAC 
TCGCACGAGC AGGGCCGTCT GGTCTATCGG TATGGCGGTG CGCCAGTCGG TGGCTTCTAT 
CCGAATCCCA CGCGTCATGA GGCGTCCAGC GTTGCACACG CCCTGTTCAT GGACCTCACC 
CACGACAATC CATCGGCCGT GGAAAAACGG TCCGTCTACG ATCTGCTGCC CTCGTCTGCT 
CTGGTCTCCA TGGCCTGCTG TGCCACCGGC AGCAATCGTG GCTACGACGA ACTGGTTCCA 
CATCATATCC ATGTCGTCGA CGAACAGCGT TTATACCAGG AATGGGGCAA GGGCGTGGAC 
TCCAAGTCCG GCATTATGGG AGCTAAGCGA GCGCTGAATC TGCTGCACGG ACAGCTGGCC 
GAAGAGGGAT TTACTCAGGT GTATGTGGAC CAAATGGACC CCAATGTGGT GGCTGTTACC 
CGGCATTCCC CGATCACCCA TCAGTCGGTC ATTCTGGTGG CTCACACGGC CTTCGGGTAT 
CCGAATCCGA ATGCCGGACC TACTGGCATC GGACCGTTGC GCTTTGACGG CGTTTTGGAC 
GAGATCATCC TGGAGGCCAG CCTGACCATG AAGAGCGACA AGCCCTTCGA TCGTCCCGCT 
CCCTTTAAGA AGGATCCCAA TGTGTTGAAC GGCTTCACCC AGTTCGAACT GAGCCTGCAG 
GAGCACATCC CGCTCAGCAA GTCGACCGTC TTTCAGACCC ATGCCAATGT CGATGGCAAC 
AACACGGTGC TGAACTTTGT TAACCTTCGG CCTGGCACAG TGGTAGCCAT TCGAGTGTCC 
ATGCATCCCG GACCCCGCAT CAGCTTCGAC AGGCTGCAGA AGCTGACAAA TGCCTTGCGC 
CTGGGATCGG GCGCTGAATG GTCAGAGCTG CAGGGGATCG TATCCAAACT GGATTTGGTG 
GCGCTGAGTG CCGCCCTCTT TCAATGTGAC GACGAGGAAC GTGCCCAAGG CACAGGTGGC 
TCCGCCTACG ATATACCCAA CTTTGGGCGC ATTGTCTACT GTGGCCTCCA GGGCTTTGTT 
TCACTGCTGA CGGAGATATC GCCACACAAT GATCTGGGCC ACCCGCTGTG CACTAATTTA 
CGCGACGGCA ACTGGATGAT GGATTACATG GCCGATCGCC TGACCAACTT CGAGGATTTA 
AAGCCGCTCT CCGTCTGGCT GAAGGCCGCC TTCGAGCCTC TGAAGAATAT ACCACGCTAC 
CTCATTCCCT GCTATTTCGA TGCCATTGTC AGCGGGGTCT ACAACGTGCT CCTTAACCAG 
GTCAACGAAC TGATGCCAGA TTTCATTAAG AACGGCCACA GTTTCACTCA GTCCCTGGCC 
CTCTCCACGT TGCAGTTCCT TTCCGTCTGC AAGTCCGCCA ATCTGCCAGG CTTCAGTCAT 
GCCATCAATC CACCAAAGCC ACCGAAACAA TGTGTTACAC TCTCTGCTGG ACTGCCGCAT 
TTCTCCACTG GTTACATGCG CTGCTGGGGC CGTGATACCT TCATCGCCCT GCGTGGATCG 
ATGTTCCTGA CTGGGCGTTT CAACGAAGCT CGATACATCA TTATTGGATT CGGCCAGACC 
CTGAGACATG GTCTCATACC CAATCTCTTG GACAGCGGGA GCAAGCCGCG CTACAACTGC 
CGCGATGCCG TCTGGTGGTG GATGTACTCC ATCAGGCAGT ACGTGGAGGA GGCTCCCAAG 
GGAAGCGAGA TACTGAAGGA CAAGGTGTCG CGCCTCTTTC CCTACGACGA TGCCGATGCA 
CATGCACCAG GGGCCTTCGA TCAGCTGCTC ATCGATGTGA TGCAGGAGGC GCTGCAGGTG 
CATTTCCAGG GACTGCAATA TCGCGAACGC AACGCGGGCC GTGAGATAGA TGAGCATATG 
GTGGACCAGG GATTCAACAA TCACATTGGA GTGCATCCGG AAACTGGCTT TGTGTTTGGC 
GGAAACAACT TTAATGCCGG CACGTGGATG GACAAGATGG GCTCGTCGCA GAAGGCCGGC 
AACAAGGGGC GGCCGAGCAC CCCACGCGAT GGATCCGCTG TGGAGTTGAT TGGCCTCCAA 
TATTCTGTAT TGCGCTTCAT GCAGGGCCTG GCCGAAAAGG AGGCCATCCC GTACACGGGT 
GTGGATCGCA AGGGGCCGTC AGGTGAGTTG ACAAAGTGGA GCTACAAGGA GTGGGCAGAT 
CGCATCAAGG ACAACTTCGA TCGCCATTTC TTTGTCCCGG AGTCAGAGAC ATGTTCCTTG 
GCAAACAAGA GGTTCATCTA CAAGGACAGC TACGGCGCAA CCCAGAGCTG GACAGACTAT 
CAGCTCCGTT GTAACTTCCC CATCACACTG ACCGTCGCTT CGGAGCTTTG CAATCCGCAG 
AACGCCTGGC GTGCTCTGGA GCTGGCCAAG CAGTACCTGC TTGGACCACT GGGCATGAAG 
ACACTGGATC CCGAGGACTG GAACTACAGG GCTAACTATG ATAACTCCAA CGACTCGACG 
GATTGCACCG TTGCCCATGG ATCCAACTAC CACCAGGGAC CCGAGTGGGT ATGGCCCATT 
GGCTTCTACC TGCGGGCTCG TCTCATCTTC GCCAAGAAAT GCGGCCATCT AGATGAAACC 
ATTGCTGAGA CCTGGGCCAT TCTGCGGGCC CATCTCCGTG AGATGCAGAC CTCCCACTGG 
CGTGGCCTGC CCGAGCTGAC CAACGACAAT GGCTCCTACT GCGGCGACTC CTGCCGCACC 
CAGGCCTGGA GCGTGGCCGC CATTCTCGAG GTTCTCTACG ATCTGCACGC CCTAGGAGCG 
GACGTAGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA21822 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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