Apc2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Apc2
Entrez Gene ID	42871
Full Name	Adenomatous polyposis coli 2
Synonyms	APC,APC2,CG6193,D-APC2,Dm APC2,Dmel\CG6193,E-APC,E-APC dAPC2,apc2,d-APC2,dAPC2,dAPC2/E-APC,dApc2,dapc2,e-apc
General protein information	Preferred Names Adenomatous polyposis coli 2 Names CG6193 gene product from transcript CG6193-RC Apc2-PA Apc2-PB Apc2-PC CG6193-PA CG6193-PB CG6193-PC adenomatous polyposis coli 2 adenomatous polyposis coli tumor suppressor homolog 2 epithelial adenomatous polyposis coli
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95E6-95E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_058082.3	NP_477430.1	adenomatous polyposis coli 2, isoform A
NM_001347814.1	NP_001334698.1	adenomatous polyposis coli 2, isoform B
NM_001347815.1	NP_001334699.1	adenomatous polyposis coli 2, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Apc2	NP_477430.1
Drosophila melanogaster (fruit fly)	Apc	NP_001263046.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Apc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Apc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05905	NM_058082.3 Latest version!	Drosophila melanogaster adenomatous polyposis coli 2, transcript variant A (Apc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OFa05905	NM_001347815.1 Latest version!	Drosophila melanogaster adenomatous polyposis coli 2, transcript variant C (Apc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OFa41227	NM_001347814.1 Latest version!	Drosophila melanogaster adenomatous polyposis coli 2, transcript variant B (Apc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Apc2 ( NM_058082.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477430.1 Drosophila melanogaster adenomatous polyposis coli 2, transcript variant A (Apc2), mRNA.

Price & Availability↑

CloneID	OFa05905
Clone ID Related Accession (Same CDS sequence)	NM_058082.3 , NM_001347815.1
Accession Version	NM_058082.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	adenomatous polyposis coli 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_058082.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCTGA ACGAGAGCGA CGCTACTCGC CTGGAGCTGA CGCGCAACTT CCTGGAACTG 
TCTCGAAATC CGGAAACATG CACCGCCCTG CGCAGCTCGG ACTGTATCCA GCTTCTGGTG 
CAGATTCTGC ACGCCAACGA CGAAGGCCTC TCCACGGCGA AAAAGTACGC CAGCCAGGCG 
CTGCACAACA TCGTCCACAA TAATCCGGAG GAGAAGGAGC GCCAGCGGGA GGTGAAGATG 
CTGCGCCTGC TGGACCAGAT CCTCGACTAC TGTAACTTTC TGCACACCCA GTTGCAGAGC 
GGCGGTGAGG CTATCGCAGA TGATGAAGAT CGTCATCCGC TGGCGGCTAT GAAGCTCCTG 
ATGAAAGCCA GTTTCGACGA GGAGCACCGC CAGACTATGT GCGAACTGGG AGCCCTCAAG 
GCGATTCCCA ATTTGGTCCA CCTTGATCAT GCGGTCCATG GACCGGCTGC CGGTAGGGAA 
CAGTGCAACG CCCTCAGGAG CTACGGCCTC ATGGCCCTCA CGAATCTCAC CTTCGGAGAC 
GAGAACGTCC ATAACAAATC GTATCTGTGC GGTCAGCGAC AGTTCATGGA AGTGGTCATT 
GCTCAATTGA ACACGGCTCC GGATGAACTG CTACAGGTTC TTGCTGGTGT GCTTCGCAAT 
CTTTCGTGGC GCGCGGACAA ACACATGAAG ACTATCTTTA ACGAGCTGGG TACTGTGACC 
TCCTTGGCTC GAGCAGCCAT GCAAAACAAG AACGAGAACA CTCTCAAGGC CATACTTTCA 
GCCCTTTGGA ATCTCTCAGC GCACTGCAGC ACCAACAAGG CGGAGTTTTG TGCAGTAGAC 
GGAGCACTGG CATTTTTGGT CGGAATGCTT AGCTACGAGG GTCCGAGTAA AACTCTTAAG 
ATCATCGAAA ATGCAGGCGG CATTCTTCGA AATGTATCGA GCCATATTGC GGTGTGTGAG 
CCGTACCGAC AAATCCTAAG ACGGTACAAT TGCCTTGCCA TTCTGTTGCA ACAGTTGAAA 
TCGGAGAGCC TAACCGTGGT AAGCAACTCC TGCGGAACTC TGTGGAATCT CTCGGCGCGC 
TGTCCCGAGG ACCAGCAATA CCTCATTGAC CACAATGCCA TCCCGCTCTT GCGGGCCCTA 
ATCAGCTCCA AGAACTCCAT GATTGCGGAG GGCAGTGCCT CGGCTTTGAA AAACCTAGTT 
AATTTCAGGG CCACTCAGGA GCTTATGCCC AATGGAGATG GTGGGTCACT GCCACTAGAC 
AAGGAAGCTG GCCATGGAGG TACGCTGCCA CGGAGATTCA GCTCACTGCG CCTAAGCTCA 
AATCCCACGG GATCGCTTAA GAAAGTGCGA CCCTCAACAG TCAGCACAAC TGGCTTTCTG 
AACAGAAAAT GTGAGAGCCG AGAGTCCATT TACTCGGGCA AATCCGATTC CACTAAATAC 
TCAACCAAGT CGGAGGGAGC GAAGAATCCC TTCGAAATTG TGACACCCAC TGAAGAGCAG 
CCCATTGACT ACTCCATGAA GTACATGGAG CACAAACCCA ATAGCAGTAA GACCTTTGAG 
ATCGACTTGG ATCAGCCAAC GGATTTCAGT GCTAGATATA AGGAGAGACG ATCCGCTCAG 
ACGGCACAGC CGGAGCTGAA GTCGGAGACC AATGAGATTA GAAGTAAGGA GTTGCAACTG 
ACAAAGTCCT CCTCGGCCAC GGAGCTGCGC AATAGTCCTG GCCTGGTGGC GGTTTCAGCA 
GCTAAGCAGA AAATTGCCAC CGAAACGGAG ACGGAAACGG CAGAGCGACC AATAAACTAC 
TGTGAAGAAG GGACTCCCGG CAGCTTCAGT CGCTTCGACT CGCTCAACAG CCTCACAGAG 
AAACCGGAGA AATGTATGCC GCCAAAAACT CCAACGAAAA CTGCGGTTCT CCCGGTGCAC 
GTTGACGGAA ATACGCCTCA AAACATCGAT TCCGCACTAG AAACTCCATT AATGTTTTCG 
CGTCGCAGCT CTATGGACTC ATTGGTTGGC GACGACGAAA CGGTTGCCTG CGAGGACAAT 
GGATCCGTGA TCAGTGAATA CAGCCGGATG CAGAGTGGTG TTATTTCCCC CTCAGAGCTG 
CCCGATTCAC CCACCCAGAG TATGCCTCAA TCTCCGAGAA GGGACAGAAA AGTGTCCACT 
CAAAATAACT TGGACACACC TGAACAGAAG CCCAGTACTG TGTTTGAGGA CAAGTTGAAC 
AGATTCCACG TGGAGCACAC GCCTGCTGCC TTCTCCTGCG CCACCAGTCT AAGTAATCTG 
AGCATGATGG ACGACTCTAA TGCAAACGCT ATTCGAGGAC AGCGTGGAAA TGACATCAAC 
GGCAATGGGG ATGCTCCTCG CAGCTATTGC ACAGAGGACA CTACTGCCGT GCTTTCCAAA 
GCGCCAAGCA ATAGTGATCT GTCCATTTTG TCCATCCCGA ATGATCTAAA TGCGAATGAA 
GCGCAGCCAG TGCCTGCTCC GCGAGCTGAT GTTACCGGAA TGGACACTCG GATGCCGGCA 
GAAGATGCAA TCTCTAAGAT GCGCTGCGGT GGCAATGCAT TGCCCAGTTA TCTGCCAGTG 
TCGGATGAAA TGAGCAAGTA CTATGTCGAG GACAGTCCCT GCACGTTTTC GGTCATCTCG 
GGACTATCCC ATCTCACTGT TGGCTCTGCT AAGGCTGGGC CTGTTCTGAA GCTGCCAATG 
AGGACTGCAG AAGAGGCTCA GGCACCCAAA CTTCCTCCCA GACGTAGTGC CGTTCAAGGA 
GATGCGGAGC CACGCTTACC GCCGAAGAAA AGCGACTCAC TGAGCTCATT GTCCATGGAC 
TCGGATGACG ACTGTAATCT TCTAAGTCAG GCCATTGCTG CGGGAAGTTG TCGACCTCAG 
CCCAGCGGTG CCAGTACCAG CTCCAGCCTG GCGAACGCTA GTACCAGCAC TCTATGCAGG 
GAAAATGGGC AGTCAAAGAA GCAGGTGGAG CATGGCGATA AGCCGAACTA CAGCTCAGAT 
GACTCGCTAG ACGACGACGA CGATGATGCA CGGTCCAAGT CGCTATTTGA GCAGTGCATT 
CTGAGCGGCA TGCATAAGTC CAACGACGCC TTGGAGTCGG AGGGTGAGCC GCCGGGGCAG 
CGCCAGGAGA TCAGTGCCCG GGATCGATTT GTCAGTAACC AGGTGCGCCA GATTGAGTCC 
ATGTTGGCTG GGCGTCAGCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477430.1
CDS	209..3412
Translation	MTLNESDATRLELTRNFLELSRNPETCTALRSSDCIQLLVQILHANDEGLSTAKKYASQALHNIVHNNPEEKERQREVKMLRLLDQILDYCNFLHTQLQSGGEAIADDEDRHPLAAMKLLMKASFDEEHRQTMCELGALKAIPNLVHLDHAVHGPAAGREQCNALRSYGLMALTNLTFGDENVHNKSYLCGQRQFMEVVIAQLNTAPDELLQVLAGVLRNLSWRADKHMKTIFNELGTVTSLARAAMQNKNENTLKAILSALWNLSAHCSTNKAEFCAVDGALAFLVGMLSYEGPSKTLKIIENAGGILRNVSSHIAVCEPYRQILRRYNCLAILLQQLKSESLTVVSNSCGTLWNLSARCPEDQQYLIDHNAIPLLRALISSKNSMIAEGSASALKNLVNFRATQELMPNGDGGSLPLDKEAGHGGTLPRRFSSLRLSSNPTGSLKKVRPSTVSTTGFLNRKCESRESIYSGKSDSTKYSTKSEGAKNPFEIVTPTEEQPIDYSMKYMEHKPNSSKTFEIDLDQPTDFSARYKERRSAQTAQPELKSETNEIRSKELQLTKSSSATELRNSPGLVAVSAAKQKIATETETETAERPINYCEEGTPGSFSRFDSLNSLTEKPEKCMPPKTPTKTAVLPVHVDGNTPQNIDSALETPLMFSRRSSMDSLVGDDETVACEDNGSVISEYSRMQSGVISPSELPDSPTQSMPQSPRRDRKVSTQNNLDTPEQKPSTVFEDKLNRFHVEHTPAAFSCATSLSNLSMMDDSNANAIRGQRGNDINGNGDAPRSYCTEDTTAVLSKAPSNSDLSILSIPNDLNANEAQPVPAPRADVTGMDTRMPAEDAISKMRCGGNALPSYLPVSDEMSKYYVEDSPCTFSVISGLSHLTVGSAKAGPVLKLPMRTAEEAQAPKLPPRRSAVQGDAEPRLPPKKSDSLSSLSMDSDDDCNLLSQAIAAGSCRPQPSGASTSSSLANASTSTLCRENGQSKKQVEHGDKPNYSSDDSLDDDDDDARSKSLFEQCILSGMHKSNDALESEGEPPGQRQEISARDRFVSNQVRQIESMLAGRQH

Target ORF information:

RefSeq Version	NM_058082.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster adenomatous polyposis coli 2, transcript variant A (Apc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058082.3

ORF Insert Sequence:

ATGACGCTGA ACGAGAGCGA CGCTACTCGC CTGGAGCTGA CGCGCAACTT CCTGGAACTG 
TCTCGAAATC CGGAAACATG CACCGCCCTG CGCAGCTCGG ACTGTATCCA GCTTCTGGTG 
CAGATTCTGC ACGCCAACGA CGAAGGCCTC TCCACGGCGA AAAAGTACGC CAGCCAGGCG 
CTGCACAACA TCGTCCACAA TAATCCGGAG GAGAAGGAGC GCCAGCGGGA GGTGAAGATG 
CTGCGCCTGC TGGACCAGAT CCTCGACTAC TGTAACTTTC TGCACACCCA GTTGCAGAGC 
GGCGGTGAGG CTATCGCAGA TGATGAAGAT CGTCATCCGC TGGCGGCTAT GAAGCTCCTG 
ATGAAAGCCA GTTTCGACGA GGAGCACCGC CAGACTATGT GCGAACTGGG AGCCCTCAAG 
GCGATTCCCA ATTTGGTCCA CCTTGATCAT GCGGTCCATG GACCGGCTGC CGGTAGGGAA 
CAGTGCAACG CCCTCAGGAG CTACGGCCTC ATGGCCCTCA CGAATCTCAC CTTCGGAGAC 
GAGAACGTCC ATAACAAATC GTATCTGTGC GGTCAGCGAC AGTTCATGGA AGTGGTCATT 
GCTCAATTGA ACACGGCTCC GGATGAACTG CTACAGGTTC TTGCTGGTGT GCTTCGCAAT 
CTTTCGTGGC GCGCGGACAA ACACATGAAG ACTATCTTTA ACGAGCTGGG TACTGTGACC 
TCCTTGGCTC GAGCAGCCAT GCAAAACAAG AACGAGAACA CTCTCAAGGC CATACTTTCA 
GCCCTTTGGA ATCTCTCAGC GCACTGCAGC ACCAACAAGG CGGAGTTTTG TGCAGTAGAC 
GGAGCACTGG CATTTTTGGT CGGAATGCTT AGCTACGAGG GTCCGAGTAA AACTCTTAAG 
ATCATCGAAA ATGCAGGCGG CATTCTTCGA AATGTATCGA GCCATATTGC GGTGTGTGAG 
CCGTACCGAC AAATCCTAAG ACGGTACAAT TGCCTTGCCA TTCTGTTGCA ACAGTTGAAA 
TCGGAGAGCC TAACCGTGGT AAGCAACTCC TGCGGAACTC TGTGGAATCT CTCGGCGCGC 
TGTCCCGAGG ACCAGCAATA CCTCATTGAC CACAATGCCA TCCCGCTCTT GCGGGCCCTA 
ATCAGCTCCA AGAACTCCAT GATTGCGGAG GGCAGTGCCT CGGCTTTGAA AAACCTAGTT 
AATTTCAGGG CCACTCAGGA GCTTATGCCC AATGGAGATG GTGGGTCACT GCCACTAGAC 
AAGGAAGCTG GCCATGGAGG TACGCTGCCA CGGAGATTCA GCTCACTGCG CCTAAGCTCA 
AATCCCACGG GATCGCTTAA GAAAGTGCGA CCCTCAACAG TCAGCACAAC TGGCTTTCTG 
AACAGAAAAT GTGAGAGCCG AGAGTCCATT TACTCGGGCA AATCCGATTC CACTAAATAC 
TCAACCAAGT CGGAGGGAGC GAAGAATCCC TTCGAAATTG TGACACCCAC TGAAGAGCAG 
CCCATTGACT ACTCCATGAA GTACATGGAG CACAAACCCA ATAGCAGTAA GACCTTTGAG 
ATCGACTTGG ATCAGCCAAC GGATTTCAGT GCTAGATATA AGGAGAGACG ATCCGCTCAG 
ACGGCACAGC CGGAGCTGAA GTCGGAGACC AATGAGATTA GAAGTAAGGA GTTGCAACTG 
ACAAAGTCCT CCTCGGCCAC GGAGCTGCGC AATAGTCCTG GCCTGGTGGC GGTTTCAGCA 
GCTAAGCAGA AAATTGCCAC CGAAACGGAG ACGGAAACGG CAGAGCGACC AATAAACTAC 
TGTGAAGAAG GGACTCCCGG CAGCTTCAGT CGCTTCGACT CGCTCAACAG CCTCACAGAG 
AAACCGGAGA AATGTATGCC GCCAAAAACT CCAACGAAAA CTGCGGTTCT CCCGGTGCAC 
GTTGACGGAA ATACGCCTCA AAACATCGAT TCCGCACTAG AAACTCCATT AATGTTTTCG 
CGTCGCAGCT CTATGGACTC ATTGGTTGGC GACGACGAAA CGGTTGCCTG CGAGGACAAT 
GGATCCGTGA TCAGTGAATA CAGCCGGATG CAGAGTGGTG TTATTTCCCC CTCAGAGCTG 
CCCGATTCAC CCACCCAGAG TATGCCTCAA TCTCCGAGAA GGGACAGAAA AGTGTCCACT 
CAAAATAACT TGGACACACC TGAACAGAAG CCCAGTACTG TGTTTGAGGA CAAGTTGAAC 
AGATTCCACG TGGAGCACAC GCCTGCTGCC TTCTCCTGCG CCACCAGTCT AAGTAATCTG 
AGCATGATGG ACGACTCTAA TGCAAACGCT ATTCGAGGAC AGCGTGGAAA TGACATCAAC 
GGCAATGGGG ATGCTCCTCG CAGCTATTGC ACAGAGGACA CTACTGCCGT GCTTTCCAAA 
GCGCCAAGCA ATAGTGATCT GTCCATTTTG TCCATCCCGA ATGATCTAAA TGCGAATGAA 
GCGCAGCCAG TGCCTGCTCC GCGAGCTGAT GTTACCGGAA TGGACACTCG GATGCCGGCA 
GAAGATGCAA TCTCTAAGAT GCGCTGCGGT GGCAATGCAT TGCCCAGTTA TCTGCCAGTG 
TCGGATGAAA TGAGCAAGTA CTATGTCGAG GACAGTCCCT GCACGTTTTC GGTCATCTCG 
GGACTATCCC ATCTCACTGT TGGCTCTGCT AAGGCTGGGC CTGTTCTGAA GCTGCCAATG 
AGGACTGCAG AAGAGGCTCA GGCACCCAAA CTTCCTCCCA GACGTAGTGC CGTTCAAGGA 
GATGCGGAGC CACGCTTACC GCCGAAGAAA AGCGACTCAC TGAGCTCATT GTCCATGGAC 
TCGGATGACG ACTGTAATCT TCTAAGTCAG GCCATTGCTG CGGGAAGTTG TCGACCTCAG 
CCCAGCGGTG CCAGTACCAG CTCCAGCCTG GCGAACGCTA GTACCAGCAC TCTATGCAGG 
GAAAATGGGC AGTCAAAGAA GCAGGTGGAG CATGGCGATA AGCCGAACTA CAGCTCAGAT 
GACTCGCTAG ACGACGACGA CGATGATGCA CGGTCCAAGT CGCTATTTGA GCAGTGCATT 
CTGAGCGGCA TGCATAAGTC CAACGACGCC TTGGAGTCGG AGGGTGAGCC GCCGGGGCAG 
CGCCAGGAGA TCAGTGCCCG GGATCGATTT GTCAGTAACC AGGTGCGCCA GATTGAGTCC 
ATGTTGGCTG GGCGTCAGCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc2 ( NM_001347815.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001334699.1 Drosophila melanogaster adenomatous polyposis coli 2, transcript variant C (Apc2), mRNA.

Price & Availability↑

CloneID	OFa05905
Clone ID Related Accession (Same CDS sequence)	NM_058082.3 , NM_001347815.1
Accession Version	NM_001347815.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	adenomatous polyposis coli 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCTGA ACGAGAGCGA CGCTACTCGC CTGGAGCTGA CGCGCAACTT CCTGGAACTG 
TCTCGAAATC CGGAAACATG CACCGCCCTG CGCAGCTCGG ACTGTATCCA GCTTCTGGTG 
CAGATTCTGC ACGCCAACGA CGAAGGCCTC TCCACGGCGA AAAAGTACGC CAGCCAGGCG 
CTGCACAACA TCGTCCACAA TAATCCGGAG GAGAAGGAGC GCCAGCGGGA GGTGAAGATG 
CTGCGCCTGC TGGACCAGAT CCTCGACTAC TGTAACTTTC TGCACACCCA GTTGCAGAGC 
GGCGGTGAGG CTATCGCAGA TGATGAAGAT CGTCATCCGC TGGCGGCTAT GAAGCTCCTG 
ATGAAAGCCA GTTTCGACGA GGAGCACCGC CAGACTATGT GCGAACTGGG AGCCCTCAAG 
GCGATTCCCA ATTTGGTCCA CCTTGATCAT GCGGTCCATG GACCGGCTGC CGGTAGGGAA 
CAGTGCAACG CCCTCAGGAG CTACGGCCTC ATGGCCCTCA CGAATCTCAC CTTCGGAGAC 
GAGAACGTCC ATAACAAATC GTATCTGTGC GGTCAGCGAC AGTTCATGGA AGTGGTCATT 
GCTCAATTGA ACACGGCTCC GGATGAACTG CTACAGGTTC TTGCTGGTGT GCTTCGCAAT 
CTTTCGTGGC GCGCGGACAA ACACATGAAG ACTATCTTTA ACGAGCTGGG TACTGTGACC 
TCCTTGGCTC GAGCAGCCAT GCAAAACAAG AACGAGAACA CTCTCAAGGC CATACTTTCA 
GCCCTTTGGA ATCTCTCAGC GCACTGCAGC ACCAACAAGG CGGAGTTTTG TGCAGTAGAC 
GGAGCACTGG CATTTTTGGT CGGAATGCTT AGCTACGAGG GTCCGAGTAA AACTCTTAAG 
ATCATCGAAA ATGCAGGCGG CATTCTTCGA AATGTATCGA GCCATATTGC GGTGTGTGAG 
CCGTACCGAC AAATCCTAAG ACGGTACAAT TGCCTTGCCA TTCTGTTGCA ACAGTTGAAA 
TCGGAGAGCC TAACCGTGGT AAGCAACTCC TGCGGAACTC TGTGGAATCT CTCGGCGCGC 
TGTCCCGAGG ACCAGCAATA CCTCATTGAC CACAATGCCA TCCCGCTCTT GCGGGCCCTA 
ATCAGCTCCA AGAACTCCAT GATTGCGGAG GGCAGTGCCT CGGCTTTGAA AAACCTAGTT 
AATTTCAGGG CCACTCAGGA GCTTATGCCC AATGGAGATG GTGGGTCACT GCCACTAGAC 
AAGGAAGCTG GCCATGGAGG TACGCTGCCA CGGAGATTCA GCTCACTGCG CCTAAGCTCA 
AATCCCACGG GATCGCTTAA GAAAGTGCGA CCCTCAACAG TCAGCACAAC TGGCTTTCTG 
AACAGAAAAT GTGAGAGCCG AGAGTCCATT TACTCGGGCA AATCCGATTC CACTAAATAC 
TCAACCAAGT CGGAGGGAGC GAAGAATCCC TTCGAAATTG TGACACCCAC TGAAGAGCAG 
CCCATTGACT ACTCCATGAA GTACATGGAG CACAAACCCA ATAGCAGTAA GACCTTTGAG 
ATCGACTTGG ATCAGCCAAC GGATTTCAGT GCTAGATATA AGGAGAGACG ATCCGCTCAG 
ACGGCACAGC CGGAGCTGAA GTCGGAGACC AATGAGATTA GAAGTAAGGA GTTGCAACTG 
ACAAAGTCCT CCTCGGCCAC GGAGCTGCGC AATAGTCCTG GCCTGGTGGC GGTTTCAGCA 
GCTAAGCAGA AAATTGCCAC CGAAACGGAG ACGGAAACGG CAGAGCGACC AATAAACTAC 
TGTGAAGAAG GGACTCCCGG CAGCTTCAGT CGCTTCGACT CGCTCAACAG CCTCACAGAG 
AAACCGGAGA AATGTATGCC GCCAAAAACT CCAACGAAAA CTGCGGTTCT CCCGGTGCAC 
GTTGACGGAA ATACGCCTCA AAACATCGAT TCCGCACTAG AAACTCCATT AATGTTTTCG 
CGTCGCAGCT CTATGGACTC ATTGGTTGGC GACGACGAAA CGGTTGCCTG CGAGGACAAT 
GGATCCGTGA TCAGTGAATA CAGCCGGATG CAGAGTGGTG TTATTTCCCC CTCAGAGCTG 
CCCGATTCAC CCACCCAGAG TATGCCTCAA TCTCCGAGAA GGGACAGAAA AGTGTCCACT 
CAAAATAACT TGGACACACC TGAACAGAAG CCCAGTACTG TGTTTGAGGA CAAGTTGAAC 
AGATTCCACG TGGAGCACAC GCCTGCTGCC TTCTCCTGCG CCACCAGTCT AAGTAATCTG 
AGCATGATGG ACGACTCTAA TGCAAACGCT ATTCGAGGAC AGCGTGGAAA TGACATCAAC 
GGCAATGGGG ATGCTCCTCG CAGCTATTGC ACAGAGGACA CTACTGCCGT GCTTTCCAAA 
GCGCCAAGCA ATAGTGATCT GTCCATTTTG TCCATCCCGA ATGATCTAAA TGCGAATGAA 
GCGCAGCCAG TGCCTGCTCC GCGAGCTGAT GTTACCGGAA TGGACACTCG GATGCCGGCA 
GAAGATGCAA TCTCTAAGAT GCGCTGCGGT GGCAATGCAT TGCCCAGTTA TCTGCCAGTG 
TCGGATGAAA TGAGCAAGTA CTATGTCGAG GACAGTCCCT GCACGTTTTC GGTCATCTCG 
GGACTATCCC ATCTCACTGT TGGCTCTGCT AAGGCTGGGC CTGTTCTGAA GCTGCCAATG 
AGGACTGCAG AAGAGGCTCA GGCACCCAAA CTTCCTCCCA GACGTAGTGC CGTTCAAGGA 
GATGCGGAGC CACGCTTACC GCCGAAGAAA AGCGACTCAC TGAGCTCATT GTCCATGGAC 
TCGGATGACG ACTGTAATCT TCTAAGTCAG GCCATTGCTG CGGGAAGTTG TCGACCTCAG 
CCCAGCGGTG CCAGTACCAG CTCCAGCCTG GCGAACGCTA GTACCAGCAC TCTATGCAGG 
GAAAATGGGC AGTCAAAGAA GCAGGTGGAG CATGGCGATA AGCCGAACTA CAGCTCAGAT 
GACTCGCTAG ACGACGACGA CGATGATGCA CGGTCCAAGT CGCTATTTGA GCAGTGCATT 
CTGAGCGGCA TGCATAAGTC CAACGACGCC TTGGAGTCGG AGGGTGAGCC GCCGGGGCAG 
CGCCAGGAGA TCAGTGCCCG GGATCGATTT GTCAGTAACC AGGTGCGCCA GATTGAGTCC 
ATGTTGGCTG GGCGTCAGCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334699.1
CDS	209..3412
Translation	MTLNESDATRLELTRNFLELSRNPETCTALRSSDCIQLLVQILHANDEGLSTAKKYASQALHNIVHNNPEEKERQREVKMLRLLDQILDYCNFLHTQLQSGGEAIADDEDRHPLAAMKLLMKASFDEEHRQTMCELGALKAIPNLVHLDHAVHGPAAGREQCNALRSYGLMALTNLTFGDENVHNKSYLCGQRQFMEVVIAQLNTAPDELLQVLAGVLRNLSWRADKHMKTIFNELGTVTSLARAAMQNKNENTLKAILSALWNLSAHCSTNKAEFCAVDGALAFLVGMLSYEGPSKTLKIIENAGGILRNVSSHIAVCEPYRQILRRYNCLAILLQQLKSESLTVVSNSCGTLWNLSARCPEDQQYLIDHNAIPLLRALISSKNSMIAEGSASALKNLVNFRATQELMPNGDGGSLPLDKEAGHGGTLPRRFSSLRLSSNPTGSLKKVRPSTVSTTGFLNRKCESRESIYSGKSDSTKYSTKSEGAKNPFEIVTPTEEQPIDYSMKYMEHKPNSSKTFEIDLDQPTDFSARYKERRSAQTAQPELKSETNEIRSKELQLTKSSSATELRNSPGLVAVSAAKQKIATETETETAERPINYCEEGTPGSFSRFDSLNSLTEKPEKCMPPKTPTKTAVLPVHVDGNTPQNIDSALETPLMFSRRSSMDSLVGDDETVACEDNGSVISEYSRMQSGVISPSELPDSPTQSMPQSPRRDRKVSTQNNLDTPEQKPSTVFEDKLNRFHVEHTPAAFSCATSLSNLSMMDDSNANAIRGQRGNDINGNGDAPRSYCTEDTTAVLSKAPSNSDLSILSIPNDLNANEAQPVPAPRADVTGMDTRMPAEDAISKMRCGGNALPSYLPVSDEMSKYYVEDSPCTFSVISGLSHLTVGSAKAGPVLKLPMRTAEEAQAPKLPPRRSAVQGDAEPRLPPKKSDSLSSLSMDSDDDCNLLSQAIAAGSCRPQPSGASTSSSLANASTSTLCRENGQSKKQVEHGDKPNYSSDDSLDDDDDDARSKSLFEQCILSGMHKSNDALESEGEPPGQRQEISARDRFVSNQVRQIESMLAGRQH

Target ORF information:

RefSeq Version	NM_001347815.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster adenomatous polyposis coli 2, transcript variant C (Apc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347815.1

ORF Insert Sequence:

ATGACGCTGA ACGAGAGCGA CGCTACTCGC CTGGAGCTGA CGCGCAACTT CCTGGAACTG 
TCTCGAAATC CGGAAACATG CACCGCCCTG CGCAGCTCGG ACTGTATCCA GCTTCTGGTG 
CAGATTCTGC ACGCCAACGA CGAAGGCCTC TCCACGGCGA AAAAGTACGC CAGCCAGGCG 
CTGCACAACA TCGTCCACAA TAATCCGGAG GAGAAGGAGC GCCAGCGGGA GGTGAAGATG 
CTGCGCCTGC TGGACCAGAT CCTCGACTAC TGTAACTTTC TGCACACCCA GTTGCAGAGC 
GGCGGTGAGG CTATCGCAGA TGATGAAGAT CGTCATCCGC TGGCGGCTAT GAAGCTCCTG 
ATGAAAGCCA GTTTCGACGA GGAGCACCGC CAGACTATGT GCGAACTGGG AGCCCTCAAG 
GCGATTCCCA ATTTGGTCCA CCTTGATCAT GCGGTCCATG GACCGGCTGC CGGTAGGGAA 
CAGTGCAACG CCCTCAGGAG CTACGGCCTC ATGGCCCTCA CGAATCTCAC CTTCGGAGAC 
GAGAACGTCC ATAACAAATC GTATCTGTGC GGTCAGCGAC AGTTCATGGA AGTGGTCATT 
GCTCAATTGA ACACGGCTCC GGATGAACTG CTACAGGTTC TTGCTGGTGT GCTTCGCAAT 
CTTTCGTGGC GCGCGGACAA ACACATGAAG ACTATCTTTA ACGAGCTGGG TACTGTGACC 
TCCTTGGCTC GAGCAGCCAT GCAAAACAAG AACGAGAACA CTCTCAAGGC CATACTTTCA 
GCCCTTTGGA ATCTCTCAGC GCACTGCAGC ACCAACAAGG CGGAGTTTTG TGCAGTAGAC 
GGAGCACTGG CATTTTTGGT CGGAATGCTT AGCTACGAGG GTCCGAGTAA AACTCTTAAG 
ATCATCGAAA ATGCAGGCGG CATTCTTCGA AATGTATCGA GCCATATTGC GGTGTGTGAG 
CCGTACCGAC AAATCCTAAG ACGGTACAAT TGCCTTGCCA TTCTGTTGCA ACAGTTGAAA 
TCGGAGAGCC TAACCGTGGT AAGCAACTCC TGCGGAACTC TGTGGAATCT CTCGGCGCGC 
TGTCCCGAGG ACCAGCAATA CCTCATTGAC CACAATGCCA TCCCGCTCTT GCGGGCCCTA 
ATCAGCTCCA AGAACTCCAT GATTGCGGAG GGCAGTGCCT CGGCTTTGAA AAACCTAGTT 
AATTTCAGGG CCACTCAGGA GCTTATGCCC AATGGAGATG GTGGGTCACT GCCACTAGAC 
AAGGAAGCTG GCCATGGAGG TACGCTGCCA CGGAGATTCA GCTCACTGCG CCTAAGCTCA 
AATCCCACGG GATCGCTTAA GAAAGTGCGA CCCTCAACAG TCAGCACAAC TGGCTTTCTG 
AACAGAAAAT GTGAGAGCCG AGAGTCCATT TACTCGGGCA AATCCGATTC CACTAAATAC 
TCAACCAAGT CGGAGGGAGC GAAGAATCCC TTCGAAATTG TGACACCCAC TGAAGAGCAG 
CCCATTGACT ACTCCATGAA GTACATGGAG CACAAACCCA ATAGCAGTAA GACCTTTGAG 
ATCGACTTGG ATCAGCCAAC GGATTTCAGT GCTAGATATA AGGAGAGACG ATCCGCTCAG 
ACGGCACAGC CGGAGCTGAA GTCGGAGACC AATGAGATTA GAAGTAAGGA GTTGCAACTG 
ACAAAGTCCT CCTCGGCCAC GGAGCTGCGC AATAGTCCTG GCCTGGTGGC GGTTTCAGCA 
GCTAAGCAGA AAATTGCCAC CGAAACGGAG ACGGAAACGG CAGAGCGACC AATAAACTAC 
TGTGAAGAAG GGACTCCCGG CAGCTTCAGT CGCTTCGACT CGCTCAACAG CCTCACAGAG 
AAACCGGAGA AATGTATGCC GCCAAAAACT CCAACGAAAA CTGCGGTTCT CCCGGTGCAC 
GTTGACGGAA ATACGCCTCA AAACATCGAT TCCGCACTAG AAACTCCATT AATGTTTTCG 
CGTCGCAGCT CTATGGACTC ATTGGTTGGC GACGACGAAA CGGTTGCCTG CGAGGACAAT 
GGATCCGTGA TCAGTGAATA CAGCCGGATG CAGAGTGGTG TTATTTCCCC CTCAGAGCTG 
CCCGATTCAC CCACCCAGAG TATGCCTCAA TCTCCGAGAA GGGACAGAAA AGTGTCCACT 
CAAAATAACT TGGACACACC TGAACAGAAG CCCAGTACTG TGTTTGAGGA CAAGTTGAAC 
AGATTCCACG TGGAGCACAC GCCTGCTGCC TTCTCCTGCG CCACCAGTCT AAGTAATCTG 
AGCATGATGG ACGACTCTAA TGCAAACGCT ATTCGAGGAC AGCGTGGAAA TGACATCAAC 
GGCAATGGGG ATGCTCCTCG CAGCTATTGC ACAGAGGACA CTACTGCCGT GCTTTCCAAA 
GCGCCAAGCA ATAGTGATCT GTCCATTTTG TCCATCCCGA ATGATCTAAA TGCGAATGAA 
GCGCAGCCAG TGCCTGCTCC GCGAGCTGAT GTTACCGGAA TGGACACTCG GATGCCGGCA 
GAAGATGCAA TCTCTAAGAT GCGCTGCGGT GGCAATGCAT TGCCCAGTTA TCTGCCAGTG 
TCGGATGAAA TGAGCAAGTA CTATGTCGAG GACAGTCCCT GCACGTTTTC GGTCATCTCG 
GGACTATCCC ATCTCACTGT TGGCTCTGCT AAGGCTGGGC CTGTTCTGAA GCTGCCAATG 
AGGACTGCAG AAGAGGCTCA GGCACCCAAA CTTCCTCCCA GACGTAGTGC CGTTCAAGGA 
GATGCGGAGC CACGCTTACC GCCGAAGAAA AGCGACTCAC TGAGCTCATT GTCCATGGAC 
TCGGATGACG ACTGTAATCT TCTAAGTCAG GCCATTGCTG CGGGAAGTTG TCGACCTCAG 
CCCAGCGGTG CCAGTACCAG CTCCAGCCTG GCGAACGCTA GTACCAGCAC TCTATGCAGG 
GAAAATGGGC AGTCAAAGAA GCAGGTGGAG CATGGCGATA AGCCGAACTA CAGCTCAGAT 
GACTCGCTAG ACGACGACGA CGATGATGCA CGGTCCAAGT CGCTATTTGA GCAGTGCATT 
CTGAGCGGCA TGCATAAGTC CAACGACGCC TTGGAGTCGG AGGGTGAGCC GCCGGGGCAG 
CGCCAGGAGA TCAGTGCCCG GGATCGATTT GTCAGTAACC AGGTGCGCCA GATTGAGTCC 
ATGTTGGCTG GGCGTCAGCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc2 ( NM_001347814.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001334698.1 Drosophila melanogaster adenomatous polyposis coli 2, transcript variant B (Apc2), mRNA.

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CloneID	OFa41227
Clone ID Related Accession (Same CDS sequence)	NM_001347814.1
Accession Version	NM_001347814.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	adenomatous polyposis coli 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCTGA ACGAGAGCGA CGCTACTCGC CTGGAGCTGA CGCGCAACTT CCTGGAACTG 
TCTCGAAATC CGGAAACATG CACCGCCCTG CGCAGCTCGG ACTGTATCCA GCTTCTGGTG 
CAGATTCTGC ACGCCAACGA CGAAGGCCTC TCCACGGCGA AAAAGTACGC CAGCCAGGCG 
CTGCACAACA TCGTCCACAA TAATCCGGAG GAGAAGGAGC GCCAGCGGGA GTTGCAGAGC 
GGCGGTGAGG CTATCGCAGA TGATGAAGAT CGTCATCCGC TGGCGGCTAT GAAGCTCCTG 
ATGAAAGCCA GTTTCGACGA GGAGCACCGC CAGACTATGT GCGAACTGGG AGCCCTCAAG 
GCGATTCCCA ATTTGGTCCA CCTTGATCAT GCGGTCCATG GACCGGCTGC CGGTAGGGAA 
CAGTGCAACG CCCTCAGGAG CTACGGCCTC ATGGCCCTCA CGAATCTCAC CTTCGGAGAC 
GAGAACGTCC ATAACAAATC GTATCTGTGC GGTCAGCGAC AGTTCATGGA AGTGGTCATT 
GCTCAATTGA ACACGGCTCC GGATGAACTG CTACAGGTTC TTGCTGGTGT GCTTCGCAAT 
CTTTCGTGGC GCGCGGACAA ACACATGAAG ACTATCTTTA ACGAGCTGGG TACTGTGACC 
TCCTTGGCTC GAGCAGCCAT GCAAAACAAG AACGAGAACA CTCTCAAGGC CATACTTTCA 
GCCCTTTGGA ATCTCTCAGC GCACTGCAGC ACCAACAAGG CGGAGTTTTG TGCAGTAGAC 
GGAGCACTGG CATTTTTGGT CGGAATGCTT AGCTACGAGG GTCCGAGTAA AACTCTTAAG 
ATCATCGAAA ATGCAGGCGG CATTCTTCGA AATGTATCGA GCCATATTGC GGTGTGTGAG 
CCGTACCGAC AAATCCTAAG ACGGTACAAT TGCCTTGCCA TTCTGTTGCA ACAGTTGAAA 
TCGGAGAGCC TAACCGTGGT AAGCAACTCC TGCGGAACTC TGTGGAATCT CTCGGCGCGC 
TGTCCCGAGG ACCAGCAATA CCTCATTGAC CACAATGCCA TCCCGCTCTT GCGGGCCCTA 
ATCAGCTCCA AGAACTCCAT GATTGCGGAG GGCAGTGCCT CGGCTTTGAA AAACCTAGTT 
AATTTCAGGG CCACTCAGGA GCTTATGCCC AATGGAGATG GTGGGTCACT GCCACTAGAC 
AAGGAAGCTG GCCATGGAGG TACGCTGCCA CGGAGATTCA GCTCACTGCG CCTAAGCTCA 
AATCCCACGG GATCGCTTAA GAAAGTGCGA CCCTCAACAG TCAGCACAAC TGGCTTTCTG 
AACAGAAAAT GTGAGAGCCG AGAGTCCATT TACTCGGGCA AATCCGATTC CACTAAATAC 
TCAACCAAGT CGGAGGGAGC GAAGAATCCC TTCGAAATTG TGACACCCAC TGAAGAGCAG 
CCCATTGACT ACTCCATGAA GTACATGGAG CACAAACCCA ATAGCAGTAA GACCTTTGAG 
ATCGACTTGG ATCAGCCAAC GGATTTCAGT GCTAGATATA AGGAGAGACG ATCCGCTCAG 
ACGGCACAGC CGGAGCTGAA GTCGGAGACC AATGAGATTA GAAGTAAGGA GTTGCAACTG 
ACAAAGTCCT CCTCGGCCAC GGAGCTGCGC AATAGTCCTG GCCTGGTGGC GGTTTCAGCA 
GCTAAGCAGA AAATTGCCAC CGAAACGGAG ACGGAAACGG CAGAGCGACC AATAAACTAC 
TGTGAAGAAG GGACTCCCGG CAGCTTCAGT CGCTTCGACT CGCTCAACAG CCTCACAGAG 
AAACCGGAGA AATGTATGCC GCCAAAAACT CCAACGAAAA CTGCGGTTCT CCCGGTGCAC 
GTTGACGGAA ATACGCCTCA AAACATCGAT TCCGCACTAG AAACTCCATT AATGTTTTCG 
CGTCGCAGCT CTATGGACTC ATTGGTTGGC GACGACGAAA CGGTTGCCTG CGAGGACAAT 
GGATCCGTGA TCAGTGAATA CAGCCGGATG CAGAGTGGTG TTATTTCCCC CTCAGAGCTG 
CCCGATTCAC CCACCCAGAG TATGCCTCAA TCTCCGAGAA GGGACAGAAA AGTGTCCACT 
CAAAATAACT TGGACACACC TGAACAGAAG CCCAGTACTG TGTTTGAGGA CAAGTTGAAC 
AGATTCCACG TGGAGCACAC GCCTGCTGCC TTCTCCTGCG CCACCAGTCT AAGTAATCTG 
AGCATGATGG ACGACTCTAA TGCAAACGCT ATTCGAGGAC AGCGTGGAAA TGACATCAAC 
GGCAATGGGG ATGCTCCTCG CAGCTATTGC ACAGAGGACA CTACTGCCGT GCTTTCCAAA 
GCGCCAAGCA ATAGTGATCT GTCCATTTTG TCCATCCCGA ATGATCTAAA TGCGAATGAA 
GCGCAGCCAG TGCCTGCTCC GCGAGCTGAT GTTACCGGAA TGGACACTCG GATGCCGGCA 
GAAGATGCAA TCTCTAAGAT GCGCTGCGGT GGCAATGCAT TGCCCAGTTA TCTGCCAGTG 
TCGGATGAAA TGAGCAAGTA CTATGTCGAG GACAGTCCCT GCACGTTTTC GGTCATCTCG 
GGACTATCCC ATCTCACTGT TGGCTCTGCT AAGGCTGGGC CTGTTCTGAA GCTGCCAATG 
AGGACTGCAG AAGAGGCTCA GGCACCCAAA CTTCCTCCCA GACGTAGTGC CGTTCAAGGA 
GATGCGGAGC CACGCTTACC GCCGAAGAAA AGCGACTCAC TGAGCTCATT GTCCATGGAC 
TCGGATGACG ACTGTAATCT TCTAAGTCAG GCCATTGCTG CGGGAAGTTG TCGACCTCAG 
CCCAGCGGTG CCAGTACCAG CTCCAGCCTG GCGAACGCTA GTACCAGCAC TCTATGCAGG 
GAAAATGGGC AGTCAAAGAA GCAGGTGGAG CATGGCGATA AGCCGAACTA CAGCTCAGAT 
GACTCGCTAG ACGACGACGA CGATGATGCA CGGTCCAAGT CGCTATTTGA GCAGTGCATT 
CTGAGCGGCA TGCATAAGTC CAACGACGCC TTGGAGTCGG AGGGTGAGCC GCCGGGGCAG 
CGCCAGGAGA TCAGTGCCCG GGATCGATTT GTCAGTAACC AGGTGCGCCA GATTGAGTCC 
ATGTTGGCTG GGCGTCAGCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334698.1
CDS	209..3352
Translation	MTLNESDATRLELTRNFLELSRNPETCTALRSSDCIQLLVQILHANDEGLSTAKKYASQALHNIVHNNPEEKERQRELQSGGEAIADDEDRHPLAAMKLLMKASFDEEHRQTMCELGALKAIPNLVHLDHAVHGPAAGREQCNALRSYGLMALTNLTFGDENVHNKSYLCGQRQFMEVVIAQLNTAPDELLQVLAGVLRNLSWRADKHMKTIFNELGTVTSLARAAMQNKNENTLKAILSALWNLSAHCSTNKAEFCAVDGALAFLVGMLSYEGPSKTLKIIENAGGILRNVSSHIAVCEPYRQILRRYNCLAILLQQLKSESLTVVSNSCGTLWNLSARCPEDQQYLIDHNAIPLLRALISSKNSMIAEGSASALKNLVNFRATQELMPNGDGGSLPLDKEAGHGGTLPRRFSSLRLSSNPTGSLKKVRPSTVSTTGFLNRKCESRESIYSGKSDSTKYSTKSEGAKNPFEIVTPTEEQPIDYSMKYMEHKPNSSKTFEIDLDQPTDFSARYKERRSAQTAQPELKSETNEIRSKELQLTKSSSATELRNSPGLVAVSAAKQKIATETETETAERPINYCEEGTPGSFSRFDSLNSLTEKPEKCMPPKTPTKTAVLPVHVDGNTPQNIDSALETPLMFSRRSSMDSLVGDDETVACEDNGSVISEYSRMQSGVISPSELPDSPTQSMPQSPRRDRKVSTQNNLDTPEQKPSTVFEDKLNRFHVEHTPAAFSCATSLSNLSMMDDSNANAIRGQRGNDINGNGDAPRSYCTEDTTAVLSKAPSNSDLSILSIPNDLNANEAQPVPAPRADVTGMDTRMPAEDAISKMRCGGNALPSYLPVSDEMSKYYVEDSPCTFSVISGLSHLTVGSAKAGPVLKLPMRTAEEAQAPKLPPRRSAVQGDAEPRLPPKKSDSLSSLSMDSDDDCNLLSQAIAAGSCRPQPSGASTSSSLANASTSTLCRENGQSKKQVEHGDKPNYSSDDSLDDDDDDARSKSLFEQCILSGMHKSNDALESEGEPPGQRQEISARDRFVSNQVRQIESMLAGRQH

Target ORF information:

RefSeq Version	NM_001347814.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster adenomatous polyposis coli 2, transcript variant B (Apc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347814.1

ORF Insert Sequence:

ATGACGCTGA ACGAGAGCGA CGCTACTCGC CTGGAGCTGA CGCGCAACTT CCTGGAACTG 
TCTCGAAATC CGGAAACATG CACCGCCCTG CGCAGCTCGG ACTGTATCCA GCTTCTGGTG 
CAGATTCTGC ACGCCAACGA CGAAGGCCTC TCCACGGCGA AAAAGTACGC CAGCCAGGCG 
CTGCACAACA TCGTCCACAA TAATCCGGAG GAGAAGGAGC GCCAGCGGGA GTTGCAGAGC 
GGCGGTGAGG CTATCGCAGA TGATGAAGAT CGTCATCCGC TGGCGGCTAT GAAGCTCCTG 
ATGAAAGCCA GTTTCGACGA GGAGCACCGC CAGACTATGT GCGAACTGGG AGCCCTCAAG 
GCGATTCCCA ATTTGGTCCA CCTTGATCAT GCGGTCCATG GACCGGCTGC CGGTAGGGAA 
CAGTGCAACG CCCTCAGGAG CTACGGCCTC ATGGCCCTCA CGAATCTCAC CTTCGGAGAC 
GAGAACGTCC ATAACAAATC GTATCTGTGC GGTCAGCGAC AGTTCATGGA AGTGGTCATT 
GCTCAATTGA ACACGGCTCC GGATGAACTG CTACAGGTTC TTGCTGGTGT GCTTCGCAAT 
CTTTCGTGGC GCGCGGACAA ACACATGAAG ACTATCTTTA ACGAGCTGGG TACTGTGACC 
TCCTTGGCTC GAGCAGCCAT GCAAAACAAG AACGAGAACA CTCTCAAGGC CATACTTTCA 
GCCCTTTGGA ATCTCTCAGC GCACTGCAGC ACCAACAAGG CGGAGTTTTG TGCAGTAGAC 
GGAGCACTGG CATTTTTGGT CGGAATGCTT AGCTACGAGG GTCCGAGTAA AACTCTTAAG 
ATCATCGAAA ATGCAGGCGG CATTCTTCGA AATGTATCGA GCCATATTGC GGTGTGTGAG 
CCGTACCGAC AAATCCTAAG ACGGTACAAT TGCCTTGCCA TTCTGTTGCA ACAGTTGAAA 
TCGGAGAGCC TAACCGTGGT AAGCAACTCC TGCGGAACTC TGTGGAATCT CTCGGCGCGC 
TGTCCCGAGG ACCAGCAATA CCTCATTGAC CACAATGCCA TCCCGCTCTT GCGGGCCCTA 
ATCAGCTCCA AGAACTCCAT GATTGCGGAG GGCAGTGCCT CGGCTTTGAA AAACCTAGTT 
AATTTCAGGG CCACTCAGGA GCTTATGCCC AATGGAGATG GTGGGTCACT GCCACTAGAC 
AAGGAAGCTG GCCATGGAGG TACGCTGCCA CGGAGATTCA GCTCACTGCG CCTAAGCTCA 
AATCCCACGG GATCGCTTAA GAAAGTGCGA CCCTCAACAG TCAGCACAAC TGGCTTTCTG 
AACAGAAAAT GTGAGAGCCG AGAGTCCATT TACTCGGGCA AATCCGATTC CACTAAATAC 
TCAACCAAGT CGGAGGGAGC GAAGAATCCC TTCGAAATTG TGACACCCAC TGAAGAGCAG 
CCCATTGACT ACTCCATGAA GTACATGGAG CACAAACCCA ATAGCAGTAA GACCTTTGAG 
ATCGACTTGG ATCAGCCAAC GGATTTCAGT GCTAGATATA AGGAGAGACG ATCCGCTCAG 
ACGGCACAGC CGGAGCTGAA GTCGGAGACC AATGAGATTA GAAGTAAGGA GTTGCAACTG 
ACAAAGTCCT CCTCGGCCAC GGAGCTGCGC AATAGTCCTG GCCTGGTGGC GGTTTCAGCA 
GCTAAGCAGA AAATTGCCAC CGAAACGGAG ACGGAAACGG CAGAGCGACC AATAAACTAC 
TGTGAAGAAG GGACTCCCGG CAGCTTCAGT CGCTTCGACT CGCTCAACAG CCTCACAGAG 
AAACCGGAGA AATGTATGCC GCCAAAAACT CCAACGAAAA CTGCGGTTCT CCCGGTGCAC 
GTTGACGGAA ATACGCCTCA AAACATCGAT TCCGCACTAG AAACTCCATT AATGTTTTCG 
CGTCGCAGCT CTATGGACTC ATTGGTTGGC GACGACGAAA CGGTTGCCTG CGAGGACAAT 
GGATCCGTGA TCAGTGAATA CAGCCGGATG CAGAGTGGTG TTATTTCCCC CTCAGAGCTG 
CCCGATTCAC CCACCCAGAG TATGCCTCAA TCTCCGAGAA GGGACAGAAA AGTGTCCACT 
CAAAATAACT TGGACACACC TGAACAGAAG CCCAGTACTG TGTTTGAGGA CAAGTTGAAC 
AGATTCCACG TGGAGCACAC GCCTGCTGCC TTCTCCTGCG CCACCAGTCT AAGTAATCTG 
AGCATGATGG ACGACTCTAA TGCAAACGCT ATTCGAGGAC AGCGTGGAAA TGACATCAAC 
GGCAATGGGG ATGCTCCTCG CAGCTATTGC ACAGAGGACA CTACTGCCGT GCTTTCCAAA 
GCGCCAAGCA ATAGTGATCT GTCCATTTTG TCCATCCCGA ATGATCTAAA TGCGAATGAA 
GCGCAGCCAG TGCCTGCTCC GCGAGCTGAT GTTACCGGAA TGGACACTCG GATGCCGGCA 
GAAGATGCAA TCTCTAAGAT GCGCTGCGGT GGCAATGCAT TGCCCAGTTA TCTGCCAGTG 
TCGGATGAAA TGAGCAAGTA CTATGTCGAG GACAGTCCCT GCACGTTTTC GGTCATCTCG 
GGACTATCCC ATCTCACTGT TGGCTCTGCT AAGGCTGGGC CTGTTCTGAA GCTGCCAATG 
AGGACTGCAG AAGAGGCTCA GGCACCCAAA CTTCCTCCCA GACGTAGTGC CGTTCAAGGA 
GATGCGGAGC CACGCTTACC GCCGAAGAAA AGCGACTCAC TGAGCTCATT GTCCATGGAC 
TCGGATGACG ACTGTAATCT TCTAAGTCAG GCCATTGCTG CGGGAAGTTG TCGACCTCAG 
CCCAGCGGTG CCAGTACCAG CTCCAGCCTG GCGAACGCTA GTACCAGCAC TCTATGCAGG 
GAAAATGGGC AGTCAAAGAA GCAGGTGGAG CATGGCGATA AGCCGAACTA CAGCTCAGAT 
GACTCGCTAG ACGACGACGA CGATGATGCA CGGTCCAAGT CGCTATTTGA GCAGTGCATT 
CTGAGCGGCA TGCATAAGTC CAACGACGCC TTGGAGTCGG AGGGTGAGCC GCCGGGGCAG 
CGCCAGGAGA TCAGTGCCCG GGATCGATTT GTCAGTAACC AGGTGCGCCA GATTGAGTCC 
ATGTTGGCTG GGCGTCAGCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Apc2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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