temp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	temp
Entrez Gene ID	31212
Full Name	tempura
Synonyms	CG3073,Dmel\CG3073,l(1)G0144
General protein information	Preferred Names tempura Names CG3073 gene product from transcript CG3073-RA CG3073-PA CG3073-PB lethal (1) G0144 temp-PA temp-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 2F2-2F2

mRNA and Protein(s)

mRNA	Protein	Name
NM_130636.3	NP_569992.1	tempura, isoform A
NM_001297889.1	NP_001284818.1	tempura, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002731	XP_563412.4
Drosophila melanogaster (fruit fly)	l(1)G0144	NP_569992.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following temp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the temp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00588	NM_001297889.1 Latest version!	Drosophila melanogaster tempura (temp), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa00588	NM_130636.3 Latest version!	Drosophila melanogaster tempura (temp), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

temp ( NM_130636.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_569992.1 Drosophila melanogaster tempura (temp), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00588
Clone ID Related Accession (Same CDS sequence)	NM_130636.3 , NM_001297889.1
Accession Version	NM_130636.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	tempura, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_130636.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAGG AACGCAACGA GAAAAAGGTG CTCTGCGAGA AGATCATCAG GGACATCAAT 
GCGGTGTTCC TCAAGGATCA GGATCTCGCC TCCTTTGAAA TTATACCCAA AGAAGCCAAC 
TGCAACAAAT CGCCGGTTGT GCATGTGGAG CACAATCTGG GCCTGGAATC GTGGTGTGCC 
CAGCATGTCT ACGACCACGC CCACCGCACC CTCATCTCCC ATCGCCGTCA AACCACGGCG 
CAACAGCTGC GGACGCTGCA GCAGCAGCAG CAGAGCGACT CCCTGGCCAA ATACCTAAAC 
GTGGCTCTGC TGATTAATCC CGATGTGACC ACCTTTTGGC ACATCCGCCG TCAGCTGGTG 
CAAAAGAATC GGCTGAGCAT CAACAAGGAG CTGCAGTTCT CCGCACTCGT GCTCTCAATC 
AAACCGAAGT CCAATGAAGC ATTCGCCTAC CGCCGTTGGC TGTACTCATT TCAAAGTGCC 
GATGCTATTG ACTGGCCCAA TGAGATCGGG ATATGCGAAA GGGCCGCCGA TAGATGCGCC 
AGCAACTATC ACGCCTGGTC GCACCGCCAG TGGATCCTTC AGAACGGCCC ATGCCTGCTG 
CAATCGGAAC TGCTACGCAC GGAGAAGTTT ATGCGGAAGC ACATCAGTGA CTACAGCTGC 
TACCACTACC GCCAAGTGCT CCTGAGTCGT GCATATGAGC TCAGTTTTGC CCTTCCCAAG 
GATTCCGGAG CCAGTGGTTC GTCTACGTTG GCCAGTCTGC AGCATCTAAT GACAAGCTAT 
GGCCTGGAAT GTGAAGCCAA CGCTGAGGAT CTGCTGGGTC TCTTGCTACC ACATGTGGAT 
CTGAGTTCCG TGAGCAAGCA AAGGCTAATA TCATTTCTTT ACTGCTGCAA CGTTGCCGCC 
AACGATATGC GTCTGTGTGC CGAGCAGCGC TTGATGTACG GATCGCGGGA TTGCTTCGAG 
CTGCATCGCC GTGCGGCACT CAAGTTCATC GTGGAGCAGT GCGTGCGCCT GCAAATGGGC 
TTGCCCAATG GCCAACTGTT ACCAGTGGCG TCAGGCGATC TGCGTTCGCA TTTGGGCAAC 
TTTGACTTTG AAGATAATCC GTTTTTAAGT GCCGTACGGC GCGAGGAGTC CACCCTGGGT 
GGTAAGCACC GGCGGTGGTG CAATCTTCAC CTCAGCTTTG GATATCCAGC GGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569992.1
CDS	480..1676
Translation	MEEERNEKKVLCEKIIRDINAVFLKDQDLASFEIIPKEANCNKSPVVHVEHNLGLESWCAQHVYDHAHRTLISHRRQTTAQQLRTLQQQQQSDSLAKYLNVALLINPDVTTFWHIRRQLVQKNRLSINKELQFSALVLSIKPKSNEAFAYRRWLYSFQSADAIDWPNEIGICERAADRCASNYHAWSHRQWILQNGPCLLQSELLRTEKFMRKHISDYSCYHYRQVLLSRAYELSFALPKDSGASGSSTLASLQHLMTSYGLECEANAEDLLGLLLPHVDLSSVSKQRLISFLYCCNVAANDMRLCAEQRLMYGSRDCFELHRRAALKFIVEQCVRLQMGLPNGQLLPVASGDLRSHLGNFDFEDNPFLSAVRREESTLGGKHRRWCNLHLSFGYPAD

Target ORF information:

RefSeq Version	NM_130636.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tempura (temp), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130636.3

ORF Insert Sequence:

ATGGAGGAGG AACGCAACGA GAAAAAGGTG CTCTGCGAGA AGATCATCAG GGACATCAAT 
GCGGTGTTCC TCAAGGATCA GGATCTCGCC TCCTTTGAAA TTATACCCAA AGAAGCCAAC 
TGCAACAAAT CGCCGGTTGT GCATGTGGAG CACAATCTGG GCCTGGAATC GTGGTGTGCC 
CAGCATGTCT ACGACCACGC CCACCGCACC CTCATCTCCC ATCGCCGTCA AACCACGGCG 
CAACAGCTGC GGACGCTGCA GCAGCAGCAG CAGAGCGACT CCCTGGCCAA ATACCTAAAC 
GTGGCTCTGC TGATTAATCC CGATGTGACC ACCTTTTGGC ACATCCGCCG TCAGCTGGTG 
CAAAAGAATC GGCTGAGCAT CAACAAGGAG CTGCAGTTCT CCGCACTCGT GCTCTCAATC 
AAACCGAAGT CCAATGAAGC ATTCGCCTAC CGCCGTTGGC TGTACTCATT TCAAAGTGCC 
GATGCTATTG ACTGGCCCAA TGAGATCGGG ATATGCGAAA GGGCCGCCGA TAGATGCGCC 
AGCAACTATC ACGCCTGGTC GCACCGCCAG TGGATCCTTC AGAACGGCCC ATGCCTGCTG 
CAATCGGAAC TGCTACGCAC GGAGAAGTTT ATGCGGAAGC ACATCAGTGA CTACAGCTGC 
TACCACTACC GCCAAGTGCT CCTGAGTCGT GCATATGAGC TCAGTTTTGC CCTTCCCAAG 
GATTCCGGAG CCAGTGGTTC GTCTACGTTG GCCAGTCTGC AGCATCTAAT GACAAGCTAT 
GGCCTGGAAT GTGAAGCCAA CGCTGAGGAT CTGCTGGGTC TCTTGCTACC ACATGTGGAT 
CTGAGTTCCG TGAGCAAGCA AAGGCTAATA TCATTTCTTT ACTGCTGCAA CGTTGCCGCC 
AACGATATGC GTCTGTGTGC CGAGCAGCGC TTGATGTACG GATCGCGGGA TTGCTTCGAG 
CTGCATCGCC GTGCGGCACT CAAGTTCATC GTGGAGCAGT GCGTGCGCCT GCAAATGGGC 
TTGCCCAATG GCCAACTGTT ACCAGTGGCG TCAGGCGATC TGCGTTCGCA TTTGGGCAAC 
TTTGACTTTG AAGATAATCC GTTTTTAAGT GCCGTACGGC GCGAGGAGTC CACCCTGGGT 
GGTAAGCACC GGCGGTGGTG CAATCTTCAC CTCAGCTTTG GATATCCAGC GGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

temp ( NM_001297889.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284818.1 Drosophila melanogaster tempura (temp), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00588
Clone ID Related Accession (Same CDS sequence)	NM_130636.3 , NM_001297889.1
Accession Version	NM_001297889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	tempura, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAGG AACGCAACGA GAAAAAGGTG CTCTGCGAGA AGATCATCAG GGACATCAAT 
GCGGTGTTCC TCAAGGATCA GGATCTCGCC TCCTTTGAAA TTATACCCAA AGAAGCCAAC 
TGCAACAAAT CGCCGGTTGT GCATGTGGAG CACAATCTGG GCCTGGAATC GTGGTGTGCC 
CAGCATGTCT ACGACCACGC CCACCGCACC CTCATCTCCC ATCGCCGTCA AACCACGGCG 
CAACAGCTGC GGACGCTGCA GCAGCAGCAG CAGAGCGACT CCCTGGCCAA ATACCTAAAC 
GTGGCTCTGC TGATTAATCC CGATGTGACC ACCTTTTGGC ACATCCGCCG TCAGCTGGTG 
CAAAAGAATC GGCTGAGCAT CAACAAGGAG CTGCAGTTCT CCGCACTCGT GCTCTCAATC 
AAACCGAAGT CCAATGAAGC ATTCGCCTAC CGCCGTTGGC TGTACTCATT TCAAAGTGCC 
GATGCTATTG ACTGGCCCAA TGAGATCGGG ATATGCGAAA GGGCCGCCGA TAGATGCGCC 
AGCAACTATC ACGCCTGGTC GCACCGCCAG TGGATCCTTC AGAACGGCCC ATGCCTGCTG 
CAATCGGAAC TGCTACGCAC GGAGAAGTTT ATGCGGAAGC ACATCAGTGA CTACAGCTGC 
TACCACTACC GCCAAGTGCT CCTGAGTCGT GCATATGAGC TCAGTTTTGC CCTTCCCAAG 
GATTCCGGAG CCAGTGGTTC GTCTACGTTG GCCAGTCTGC AGCATCTAAT GACAAGCTAT 
GGCCTGGAAT GTGAAGCCAA CGCTGAGGAT CTGCTGGGTC TCTTGCTACC ACATGTGGAT 
CTGAGTTCCG TGAGCAAGCA AAGGCTAATA TCATTTCTTT ACTGCTGCAA CGTTGCCGCC 
AACGATATGC GTCTGTGTGC CGAGCAGCGC TTGATGTACG GATCGCGGGA TTGCTTCGAG 
CTGCATCGCC GTGCGGCACT CAAGTTCATC GTGGAGCAGT GCGTGCGCCT GCAAATGGGC 
TTGCCCAATG GCCAACTGTT ACCAGTGGCG TCAGGCGATC TGCGTTCGCA TTTGGGCAAC 
TTTGACTTTG AAGATAATCC GTTTTTAAGT GCCGTACGGC GCGAGGAGTC CACCCTGGGT 
GGTAAGCACC GGCGGTGGTG CAATCTTCAC CTCAGCTTTG GATATCCAGC GGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284818.1
CDS	108..1304
Translation	MEEERNEKKVLCEKIIRDINAVFLKDQDLASFEIIPKEANCNKSPVVHVEHNLGLESWCAQHVYDHAHRTLISHRRQTTAQQLRTLQQQQQSDSLAKYLNVALLINPDVTTFWHIRRQLVQKNRLSINKELQFSALVLSIKPKSNEAFAYRRWLYSFQSADAIDWPNEIGICERAADRCASNYHAWSHRQWILQNGPCLLQSELLRTEKFMRKHISDYSCYHYRQVLLSRAYELSFALPKDSGASGSSTLASLQHLMTSYGLECEANAEDLLGLLLPHVDLSSVSKQRLISFLYCCNVAANDMRLCAEQRLMYGSRDCFELHRRAALKFIVEQCVRLQMGLPNGQLLPVASGDLRSHLGNFDFEDNPFLSAVRREESTLGGKHRRWCNLHLSFGYPAD

Target ORF information:

RefSeq Version	NM_001297889.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tempura (temp), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297889.1

ORF Insert Sequence:

ATGGAGGAGG AACGCAACGA GAAAAAGGTG CTCTGCGAGA AGATCATCAG GGACATCAAT 
GCGGTGTTCC TCAAGGATCA GGATCTCGCC TCCTTTGAAA TTATACCCAA AGAAGCCAAC 
TGCAACAAAT CGCCGGTTGT GCATGTGGAG CACAATCTGG GCCTGGAATC GTGGTGTGCC 
CAGCATGTCT ACGACCACGC CCACCGCACC CTCATCTCCC ATCGCCGTCA AACCACGGCG 
CAACAGCTGC GGACGCTGCA GCAGCAGCAG CAGAGCGACT CCCTGGCCAA ATACCTAAAC 
GTGGCTCTGC TGATTAATCC CGATGTGACC ACCTTTTGGC ACATCCGCCG TCAGCTGGTG 
CAAAAGAATC GGCTGAGCAT CAACAAGGAG CTGCAGTTCT CCGCACTCGT GCTCTCAATC 
AAACCGAAGT CCAATGAAGC ATTCGCCTAC CGCCGTTGGC TGTACTCATT TCAAAGTGCC 
GATGCTATTG ACTGGCCCAA TGAGATCGGG ATATGCGAAA GGGCCGCCGA TAGATGCGCC 
AGCAACTATC ACGCCTGGTC GCACCGCCAG TGGATCCTTC AGAACGGCCC ATGCCTGCTG 
CAATCGGAAC TGCTACGCAC GGAGAAGTTT ATGCGGAAGC ACATCAGTGA CTACAGCTGC 
TACCACTACC GCCAAGTGCT CCTGAGTCGT GCATATGAGC TCAGTTTTGC CCTTCCCAAG 
GATTCCGGAG CCAGTGGTTC GTCTACGTTG GCCAGTCTGC AGCATCTAAT GACAAGCTAT 
GGCCTGGAAT GTGAAGCCAA CGCTGAGGAT CTGCTGGGTC TCTTGCTACC ACATGTGGAT 
CTGAGTTCCG TGAGCAAGCA AAGGCTAATA TCATTTCTTT ACTGCTGCAA CGTTGCCGCC 
AACGATATGC GTCTGTGTGC CGAGCAGCGC TTGATGTACG GATCGCGGGA TTGCTTCGAG 
CTGCATCGCC GTGCGGCACT CAAGTTCATC GTGGAGCAGT GCGTGCGCCT GCAAATGGGC 
TTGCCCAATG GCCAACTGTT ACCAGTGGCG TCAGGCGATC TGCGTTCGCA TTTGGGCAAC 
TTTGACTTTG AAGATAATCC GTTTTTAAGT GCCGTACGGC GCGAGGAGTC CACCCTGGGT 
GGTAAGCACC GGCGGTGGTG CAATCTTCAC CTCAGCTTTG GATATCCAGC GGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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temp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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