KDM5C cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	KDM5C
Entrez Gene ID	553406
Full Name	lysine (K)-specific demethylase 5C
Synonyms	im:7158173,si:ch211-218o21.2,wu:fa28h03,wu:fi31b07
General protein information	Preferred Names lysine (K)-specific demethylase 5C Names lysine-specific demethylase 5C smcx
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017357332.2	XP_017212821.1	lysine-specific demethylase 5C isoform X1
NM_001123234.1	NP_001116706.1	lysine-specific demethylase 5C
XM_017357332.1	XP_017212821.1	lysine-specific demethylase 5C isoform X1

Pathways from BioSystems

Homologies

Neurospora crassa	NCU01238	XP_961604.1
Xenopus tropicalis (tropical clawed frog)	kdm5c	NP_001072719.1
Homo sapiens (human)	KDM5C	NP_001269551.1
Magnaporthe oryzae (rice blast fungus)	MGG_04878	XP_003712359.1
Canis lupus familiaris (dog)	KDM5C	NP_001041497.1
Mus musculus (house mouse)	Kdm5c	NP_038696.2
Bos taurus (cattle)	KDM5C	NP_776610.2
Rattus norvegicus (Norway rat)	Kdm5c	XP_006257102.1
Macaca mulatta (Rhesus monkey)	KDM5C	XP_002806302.1
Schizosaccharomyces pombe (fission yeast)	lid2	NP_596174.1
Schizosaccharomyces pombe (fission yeast)	msc1	NP_593431.1
Danio rerio (zebrafish)	kdm5c	NP_001116706.1

Gene Ontology
Bibliography

Related articles in PubMed

The Kdm/Kmt gene families in the self-fertilizing mangrove rivulus fish, Kryptolebias marmoratus, suggest involvement of histone methylation machinery in development and reproduction.
Fellous A, Earley RL, Silvestre F
Gene687173-187(2019 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

KDM7 is a dual demethylase for histone H3 Lys 9 and Lys 27 and functions in brain development.
Tsukada Y, Ishitani T, Nakayama KI
Genes & development24(5)432-7(2010 Mar)

The X-linked mental retardation gene SMCX/JARID1C defines a family of histone H3 lysine 4 demethylases.
Iwase S, Lan F, Bayliss P, de la Torre-Ubieta L, Huarte M, Qi HH, Whetstine JR, Bonni A, Roberts TM, Shi Y
Cell128(6)1077-88(2007 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KDM5C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KDM5C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa30505	NM_001123234.1 Latest version!	Danio rerio lysine (K)-specific demethylase 5C (kdm5c), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa41943	XM_017357332.2 Latest version!	Danio rerio lysine (K)-specific demethylase 5C (kdm5c), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
ODa41943	XM_017357332.1	Danio rerio lysine (K)-specific demethylase 5C (kdm5c), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KDM5C ( NM_001123234.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001116706.1 Danio rerio lysine (K)-specific demethylase 5C (kdm5c), mRNA.

Price & Availability↑

CloneID	ODa30505
Clone ID Related Accession (Same CDS sequence)	NM_001123234.1
Accession Version	NM_001123234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559836800000
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 5C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX537348.8. On Apr 11, 2008 this sequence version replaced XM_704687.2. ##Evidence-Data-START## Transcript exon combination :: GFIL01013913.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGATGGATG TAGGGGAGGA GTTTGTTCCA CCACCTGAAT GTCCAGTATT TGAACCATCA 
TGGGAGGAGT TTGCAGATCC ATTGGGATAT ATTGCCAAAA TCCGTCCAAT AGCAGAAAAG 
TCTGGAATAT GCAAGATTCG TCCACCACCC GACTGGCAGC CACCGTTTGC TGTGGAGGTG 
GACAATTTTC ATTTCACACC CCGTATTCAG AGGCTCAATG AGCTAGAGGC TGAGACACGA 
GTAAAGCTGA ATTACCTAGA CAGAATTGCC AAGTTCTGGG AGATCCAGGG ATCCTCTCTG 
AAGATCCCCA ATTTAGAGCG ACGTATTCTG GATCTGTTCA GCTTGGCCAA GATTGTCACA 
GAGGAAGGGG GCTTTGAGTC TGTGAGTAAG GAAAGGCGGT GGGCTCGTGT GGCACAGAAA 
CTCGGTTATC CTCCTGGTAA AAACATTGGC TCTCTCCTGA GGTCCCACTA TGAGAGGATT 
GTGTATCCAT TTGAGTTGTT CCAGTCTGGA GCCAGTTTAC CGCCACGTAA ACCCAGGCAA 
TATGACAGTG ATGAGGTAGA CCGAGAATAC AAACCCCACT CCATTCCTTT GCGTCAGTCA 
GTGCCACCAT CCAAGATGAG CAGCTATGGA CGAAGGGCCA ATCGCCTTCA GCCTGAGGGT 
CCCGAGGATC CAACTCACCA CCCTCTTACA ACTGGCTCTC AACACATCTC CTCGCCGGAG 
CCTACAGAAG AAGACATTGA GAAGAACCCT GAGCTGAAAA AGCTACAGAT CTATGGGGCT 
GGACCTAAAA TGATGGGCCT TGGTCTTGTA CCTCGAGACA AGACGCGGAA GAAAGATGAT 
CTGCCGCAGA CTGTAATTGT GCGGGACAGT GCTGTGAAGG ATGAGCCTGC AGAGACGGCG 
GTTACAAAAT CAGATCCAGA CATTCCACCG CCACCCCCAA ATCTCATCAT CAAGGAGGAT 
GTCGATGATG ACAAAAGTCA TATTGACAAA CCTAACGATC CCAGTGATGA GCCTTGCACT 
AAGATGACCA TGAGACTCCG ACGCAACCTC AATAATCCAC AGTTTGTGGA CTCGTTTGTG 
TGTCGAATGT GTGGACGCGG GGATGAGGAC GAGAAGTTGA TGCTCTGTGA TGGGTGTGAT 
GATAATTACC ACACATTCTG CCTGATACCT CCACTCACAG ACCCACCCAA GGGCAACTGG 
CGCTGTCCCA AATGCGTAGC AGAGGAGTGT AAAAAGCCAT CAGAGGCATT CGGCTTCGAA 
CAGGCCACAA GAGAGTACAC GTTACAGAGC TTCGGTGAAA TGGCCGACAC TTTCAAAGCA 
GACTACTTCA ACATGCCTGT CCATATGGTT CCCACAGAGC TGGTTGAAAA GGAGTTTTGG 
AGGTTGGTTA GCAGTATTGA AGAAGATGTA ACTGTAGAAT ATGGTGCCGA TATTCACTCA 
AAAGAATTTG GTAGTGGTTT TCCTGTCAAC AACGGCAAGA GACAATTATC AGAGGAGGAA 
GAGGAATATG CCCGCAGCGG CTGGAACCTA AATGTTATGC CAGTGTTGGA ACAGTCATTG 
TTGTGCCACA TCAATGCAGA TATCTCTGGC ATGAAGGTGC CATGGCTATA TGTGGGCATG 
GTGTTCTCTG CTTTCTGTTG GCACATAGAG GACCACTGGA GTTACTCCAT CAATTATTTA 
CACTGGGGCG AGCCCAAAAC ATGGTATGGA GTTCCATGTT CAGCTGCAGA GAAGTTGGAG 
GAGGTGATGA AGAAACTCAC ACCTGAGCTA TTTGAGTTCC AGCCAGACCT GCTCCACCAG 
CTAGTCACCA TCATGAACCC AAATATACTC ATGAGTCATG GAGTGCCGGT GGTTCGGACC 
AATCAGTGTG CTGGGGAGTT TGTCATCACC TTTCCACGAG CTTACCACAG CGGTTTTAAT 
CAGGGATATA ACTTTGCCGA AGCTGTCAAC TTCTGCACTG CAGATTGGTT GCCCACAGGG 
CGCTCTTGTA TTGAGCATTA CCGGCAGCTG AGGCGCTACT GTGTGTTCTC TCAAGAGGAA 
CTCACCTGCA AGATGGCGGC CTGCCCAGAA AAGCTGGATC TGAACCTGGC AGCCGCCACA 
CACAGGGAGA TGTTCATCAT AGTGCAGGAA GAGAGGAAAC TACGCAAAAG CCTACTGGAA 
AGGGGCATCA AGGAAGCTGA AAGGGAGGCC TTTGAGTTGC TGCCTGATGA TGAACGACAG 
TGTGACAAAT GTAAGACCAC CTGCTTCCTC TCAGCTCTGG CATGTTCGAA CTGCCCAGAG 
CGTCTCGTCT GCCTCTATCA TGCCCAGGAC CTCTGCTCTT GCCCGAGTGA AAAACTCTAC 
CTCAGGTATC GCTATACTCT GGATGAACTG TTGGCCATGT TGCATCGGTT GAAAGTTCGC 
GCTGAGTCTT TTGACTCTTG GGCTAACCGC GTGAAGGAAG CCCTAGAGCA GGATGAAGGC 
AACAAAATAG ATATTAAAGA TCTGGAGGTA TTAAAAGAAG AGGCAGCTGA CAAGAAGTTC 
CCCAATAATG AGCTGCTCCA GCGCCTCAAC ACTGTCTTCG TGGACATAGA GAAGTGCGAG 
AGCTGCAGCA CAGAACTCCT CAGCAACAGC CAGAGCAGTA GAATGACCCT GAAGGAGCTG 
AAGACTCTGG TGGACACCAT GCAGAACCTG CCATGTGTTA TAAAGCAGCA GGACGAAGTT 
CAGCTGATAT TGCAGAAGAT AGAGGAGTTT GATTCCCGTG CTCAAGTTCT GGTCGACAGC 
ACCAAGAGTG AATGGAAGCA GGACTCTCCT CTTCCTGTGG CCACTGAGAT CCAGGCTCTG 
CTAGAGGAAG GTGCATCACT ACCGGTTATT GCCCCAGCTT GTGAGCTTTT GAGTGGGCTG 
TTGGAGCAGG GGCGCTGGCT GGGAGAGGTC AGGCGGACGT TAGGGCCAGA GGGCAGTGAG 
GTGACTCTGA CTGTGCTAAG GAACTTGATG GAGTCGGGCT GTAATGTACC ACAGAGTGTG 
TCAGTGGAGA CTGCAATGGC TGAACTGCAA GAGCTCTTGA CAATAGCTGA ACGCTGGGAG 
GAGAAGGCGC AGATATGCTT GGAGGACAGG CAGAAACACC ATTTATCAAC GCTGGAGGCT 
ATTGTGAATG AGGCTCAACT AATACCAGTC CAGCTGCCTA ATATCCTGTC CCTACAGGCC 
TGCTTGAGCC GAGCTCGGGC TTGGGTCACA GATCTGGAGG AAATTCAGAA TGGTGAGCAC 
TATCCATGTT TGGATGACCT GGAGGGTCTG GTAGCCATTG GGAGGGACTT GCCAGTGAAG 
ATGGAGGAGC TGAAGCAGCT GGAGCTGCAG GTGGCCAGTG CTCACTCCTG GAGAGAGAAG 
GCCAGCAAAA CCTTTCTGAA GAAGAACAGC CAACATAGTC TGCTCGAGGT GCTGTGCCCA 
TGTGTTGAGA AGAGCAAGAA GATGGAGGAC AACTCAATGG TTACAGAAGC AGATTCGGAT 
GTCAATGTAT TAGGCCTCAC AGCTCAGGAC CTCAGAGACC CAGGAGCTAT TGTAATGGCA 
TTTAAAGAAG GGGAGCGTCT GGAGAAAGAA GCCCTCCTCC GCCTCCAGCA GGTTAATTTG 
GCGAAACCCA GTATCGAAAA TGGAGTAAGC ACTGACCAGG ACAGTATCAA GACAGAAAAG 
AAACAGAGTA ACTCCATGGA GACAGATGTG CCCCTTAACT CAGACAGTCC CGTGCTGCAA 
AATGGAGGCT CTTCCCATTC CCTCACCTCT AGCCAAACTG TGTGTATTTG TGGTGGCCCA 
CCTCGCCCCT TGCTTCACCG GTGCCATCTG TGCAAAGACT GGTTCCATGG AGGTTGTGTG 
TCCTTCCCAC CATTGTTGGC CACTTCAGAC ACGCATCTTA CAAAACCCCT CTGCTGGTGG 
GACTGGAACA CGCGTTTTCT CTGTCCGCGG TGCCAGCGAT CCCGACGGCC ACGGCTGGAA 
ACTATTCTAG CGCTGCTGGT GGCGCTGCAG AGGTTACCTG TACGGCTGCC AGAGGGAGAA 
GCGTTACAGT GTCTTACAGA GAGAGCTATT AACTGGCAAG GTCGAGCCAA ACAGGCATTA 
GACACTCCAG AAGTACAGGA GGCGCTAGAC AGACTGCAAC AAGTAGAGGA TGAAATGGTC 
AGTATAAAAG AGGAAGAACC AGAGGAGAAA AAGAAATGGA ATGGAGATGC TGTAATTGTG 
CTTTCTGACT CGGAGGAAGC AGAAGATGGT GTTATCGATC TGACGGAAGA TGGGAACTCG 
CCAAAAAAGA ATGAAAGGAA CGCTGTCAAT GGTACACAGT CTGGCTGTGA AAATGGAATT 
GGCAGGAAGT CGAGTGCACA TGAAGTTACA GGGGTGGAGT CTCTGTTGTC ACTAGTGCCG 
AGTCTTAAAG GGCCAGTGGT GGAGCTGAAC ACATCTGTCA GAGCACAGCT GGAGGAGCTT 
CAGCTTGAAG GAGACCTGCT GGAGGTCACG CTTGACCAGA CACACGCCAT CTACCGCATC 
CTTCAGGCAT CATCTCAGCC GCCCCAGGAC ACCTTGCGGA CACTCATACA GATTGAACTG 
CAGGAGCAGA GAAGCTCAGG TCGTGGCAAC AAGTCGAAAG ACTCCAAGAG GAAGAGAAAG 
AGCCAGAAAA CCGAAGGCGA GCCGAAATCC ACAGAGGCTT CAGACTGCAA GAAGACGAAA 
GCCCTCAATC TCAGCCCACC TACAGACACA CACACAGATG TTGTGATGTT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116706.1
CDS	423..5159
Translation	MMDVGEEFVPPPECPVFEPSWEEFADPLGYIAKIRPIAEKSGICKIRPPPDWQPPFAVEVDNFHFTPRIQRLNELEAETRVKLNYLDRIAKFWEIQGSSLKIPNLERRILDLFSLAKIVTEEGGFESVSKERRWARVAQKLGYPPGKNIGSLLRSHYERIVYPFELFQSGASLPPRKPRQYDSDEVDREYKPHSIPLRQSVPPSKMSSYGRRANRLQPEGPEDPTHHPLTTGSQHISSPEPTEEDIEKNPELKKLQIYGAGPKMMGLGLVPRDKTRKKDDLPQTVIVRDSAVKDEPAETAVTKSDPDIPPPPPNLIIKEDVDDDKSHIDKPNDPSDEPCTKMTMRLRRNLNNPQFVDSFVCRMCGRGDEDEKLMLCDGCDDNYHTFCLIPPLTDPPKGNWRCPKCVAEECKKPSEAFGFEQATREYTLQSFGEMADTFKADYFNMPVHMVPTELVEKEFWRLVSSIEEDVTVEYGADIHSKEFGSGFPVNNGKRQLSEEEEEYARSGWNLNVMPVLEQSLLCHINADISGMKVPWLYVGMVFSAFCWHIEDHWSYSINYLHWGEPKTWYGVPCSAAEKLEEVMKKLTPELFEFQPDLLHQLVTIMNPNILMSHGVPVVRTNQCAGEFVITFPRAYHSGFNQGYNFAEAVNFCTADWLPTGRSCIEHYRQLRRYCVFSQEELTCKMAACPEKLDLNLAAATHREMFIIVQEERKLRKSLLERGIKEAEREAFELLPDDERQCDKCKTTCFLSALACSNCPERLVCLYHAQDLCSCPSEKLYLRYRYTLDELLAMLHRLKVRAESFDSWANRVKEALEQDEGNKIDIKDLEVLKEEAADKKFPNNELLQRLNTVFVDIEKCESCSTELLSNSQSSRMTLKELKTLVDTMQNLPCVIKQQDEVQLILQKIEEFDSRAQVLVDSTKSEWKQDSPLPVATEIQALLEEGASLPVIAPACELLSGLLEQGRWLGEVRRTLGPEGSEVTLTVLRNLMESGCNVPQSVSVETAMAELQELLTIAERWEEKAQICLEDRQKHHLSTLEAIVNEAQLIPVQLPNILSLQACLSRARAWVTDLEEIQNGEHYPCLDDLEGLVAIGRDLPVKMEELKQLELQVASAHSWREKASKTFLKKNSQHSLLEVLCPCVEKSKKMEDNSMVTEADSDVNVLGLTAQDLRDPGAIVMAFKEGERLEKEALLRLQQVNLAKPSIENGVSTDQDSIKTEKKQSNSMETDVPLNSDSPVLQNGGSSHSLTSSQTVCICGGPPRPLLHRCHLCKDWFHGGCVSFPPLLATSDTHLTKPLCWWDWNTRFLCPRCQRSRRPRLETILALLVALQRLPVRLPEGEALQCLTERAINWQGRAKQALDTPEVQEALDRLQQVEDEMVSIKEEEPEEKKKWNGDAVIVLSDSEEAEDGVIDLTEDGNSPKKNERNAVNGTQSGCENGIGRKSSAHEVTGVESLLSLVPSLKGPVVELNTSVRAQLEELQLEGDLLEVTLDQTHAIYRILQASSQPPQDTLRTLIQIELQEQRSSGRGNKSKDSKRKRKSQKTEGEPKSTEASDCKKTKALNLSPPTDTHTDVVMFS

Target ORF information:

RefSeq Version	NM_001123234.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 5C (kdm5c), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123234.1

ORF Insert Sequence:

ATGATGGATG TAGGGGAGGA GTTTGTTCCA CCACCTGAAT GTCCAGTATT TGAACCATCA 
TGGGAGGAGT TTGCAGATCC ATTGGGATAT ATTGCCAAAA TCCGTCCAAT AGCAGAAAAG 
TCTGGAATAT GCAAGATTCG TCCACCACCC GACTGGCAGC CACCGTTTGC TGTGGAGGTG 
GACAATTTTC ATTTCACACC CCGTATTCAG AGGCTCAATG AGCTAGAGGC TGAGACACGA 
GTAAAGCTGA ATTACCTAGA CAGAATTGCC AAGTTCTGGG AGATCCAGGG ATCCTCTCTG 
AAGATCCCCA ATTTAGAGCG ACGTATTCTG GATCTGTTCA GCTTGGCCAA GATTGTCACA 
GAGGAAGGGG GCTTTGAGTC TGTGAGTAAG GAAAGGCGGT GGGCTCGTGT GGCACAGAAA 
CTCGGTTATC CTCCTGGTAA AAACATTGGC TCTCTCCTGA GGTCCCACTA TGAGAGGATT 
GTGTATCCAT TTGAGTTGTT CCAGTCTGGA GCCAGTTTAC CGCCACGTAA ACCCAGGCAA 
TATGACAGTG ATGAGGTAGA CCGAGAATAC AAACCCCACT CCATTCCTTT GCGTCAGTCA 
GTGCCACCAT CCAAGATGAG CAGCTATGGA CGAAGGGCCA ATCGCCTTCA GCCTGAGGGT 
CCCGAGGATC CAACTCACCA CCCTCTTACA ACTGGCTCTC AACACATCTC CTCGCCGGAG 
CCTACAGAAG AAGACATTGA GAAGAACCCT GAGCTGAAAA AGCTACAGAT CTATGGGGCT 
GGACCTAAAA TGATGGGCCT TGGTCTTGTA CCTCGAGACA AGACGCGGAA GAAAGATGAT 
CTGCCGCAGA CTGTAATTGT GCGGGACAGT GCTGTGAAGG ATGAGCCTGC AGAGACGGCG 
GTTACAAAAT CAGATCCAGA CATTCCACCG CCACCCCCAA ATCTCATCAT CAAGGAGGAT 
GTCGATGATG ACAAAAGTCA TATTGACAAA CCTAACGATC CCAGTGATGA GCCTTGCACT 
AAGATGACCA TGAGACTCCG ACGCAACCTC AATAATCCAC AGTTTGTGGA CTCGTTTGTG 
TGTCGAATGT GTGGACGCGG GGATGAGGAC GAGAAGTTGA TGCTCTGTGA TGGGTGTGAT 
GATAATTACC ACACATTCTG CCTGATACCT CCACTCACAG ACCCACCCAA GGGCAACTGG 
CGCTGTCCCA AATGCGTAGC AGAGGAGTGT AAAAAGCCAT CAGAGGCATT CGGCTTCGAA 
CAGGCCACAA GAGAGTACAC GTTACAGAGC TTCGGTGAAA TGGCCGACAC TTTCAAAGCA 
GACTACTTCA ACATGCCTGT CCATATGGTT CCCACAGAGC TGGTTGAAAA GGAGTTTTGG 
AGGTTGGTTA GCAGTATTGA AGAAGATGTA ACTGTAGAAT ATGGTGCCGA TATTCACTCA 
AAAGAATTTG GTAGTGGTTT TCCTGTCAAC AACGGCAAGA GACAATTATC AGAGGAGGAA 
GAGGAATATG CCCGCAGCGG CTGGAACCTA AATGTTATGC CAGTGTTGGA ACAGTCATTG 
TTGTGCCACA TCAATGCAGA TATCTCTGGC ATGAAGGTGC CATGGCTATA TGTGGGCATG 
GTGTTCTCTG CTTTCTGTTG GCACATAGAG GACCACTGGA GTTACTCCAT CAATTATTTA 
CACTGGGGCG AGCCCAAAAC ATGGTATGGA GTTCCATGTT CAGCTGCAGA GAAGTTGGAG 
GAGGTGATGA AGAAACTCAC ACCTGAGCTA TTTGAGTTCC AGCCAGACCT GCTCCACCAG 
CTAGTCACCA TCATGAACCC AAATATACTC ATGAGTCATG GAGTGCCGGT GGTTCGGACC 
AATCAGTGTG CTGGGGAGTT TGTCATCACC TTTCCACGAG CTTACCACAG CGGTTTTAAT 
CAGGGATATA ACTTTGCCGA AGCTGTCAAC TTCTGCACTG CAGATTGGTT GCCCACAGGG 
CGCTCTTGTA TTGAGCATTA CCGGCAGCTG AGGCGCTACT GTGTGTTCTC TCAAGAGGAA 
CTCACCTGCA AGATGGCGGC CTGCCCAGAA AAGCTGGATC TGAACCTGGC AGCCGCCACA 
CACAGGGAGA TGTTCATCAT AGTGCAGGAA GAGAGGAAAC TACGCAAAAG CCTACTGGAA 
AGGGGCATCA AGGAAGCTGA AAGGGAGGCC TTTGAGTTGC TGCCTGATGA TGAACGACAG 
TGTGACAAAT GTAAGACCAC CTGCTTCCTC TCAGCTCTGG CATGTTCGAA CTGCCCAGAG 
CGTCTCGTCT GCCTCTATCA TGCCCAGGAC CTCTGCTCTT GCCCGAGTGA AAAACTCTAC 
CTCAGGTATC GCTATACTCT GGATGAACTG TTGGCCATGT TGCATCGGTT GAAAGTTCGC 
GCTGAGTCTT TTGACTCTTG GGCTAACCGC GTGAAGGAAG CCCTAGAGCA GGATGAAGGC 
AACAAAATAG ATATTAAAGA TCTGGAGGTA TTAAAAGAAG AGGCAGCTGA CAAGAAGTTC 
CCCAATAATG AGCTGCTCCA GCGCCTCAAC ACTGTCTTCG TGGACATAGA GAAGTGCGAG 
AGCTGCAGCA CAGAACTCCT CAGCAACAGC CAGAGCAGTA GAATGACCCT GAAGGAGCTG 
AAGACTCTGG TGGACACCAT GCAGAACCTG CCATGTGTTA TAAAGCAGCA GGACGAAGTT 
CAGCTGATAT TGCAGAAGAT AGAGGAGTTT GATTCCCGTG CTCAAGTTCT GGTCGACAGC 
ACCAAGAGTG AATGGAAGCA GGACTCTCCT CTTCCTGTGG CCACTGAGAT CCAGGCTCTG 
CTAGAGGAAG GTGCATCACT ACCGGTTATT GCCCCAGCTT GTGAGCTTTT GAGTGGGCTG 
TTGGAGCAGG GGCGCTGGCT GGGAGAGGTC AGGCGGACGT TAGGGCCAGA GGGCAGTGAG 
GTGACTCTGA CTGTGCTAAG GAACTTGATG GAGTCGGGCT GTAATGTACC ACAGAGTGTG 
TCAGTGGAGA CTGCAATGGC TGAACTGCAA GAGCTCTTGA CAATAGCTGA ACGCTGGGAG 
GAGAAGGCGC AGATATGCTT GGAGGACAGG CAGAAACACC ATTTATCAAC GCTGGAGGCT 
ATTGTGAATG AGGCTCAACT AATACCAGTC CAGCTGCCTA ATATCCTGTC CCTACAGGCC 
TGCTTGAGCC GAGCTCGGGC TTGGGTCACA GATCTGGAGG AAATTCAGAA TGGTGAGCAC 
TATCCATGTT TGGATGACCT GGAGGGTCTG GTAGCCATTG GGAGGGACTT GCCAGTGAAG 
ATGGAGGAGC TGAAGCAGCT GGAGCTGCAG GTGGCCAGTG CTCACTCCTG GAGAGAGAAG 
GCCAGCAAAA CCTTTCTGAA GAAGAACAGC CAACATAGTC TGCTCGAGGT GCTGTGCCCA 
TGTGTTGAGA AGAGCAAGAA GATGGAGGAC AACTCAATGG TTACAGAAGC AGATTCGGAT 
GTCAATGTAT TAGGCCTCAC AGCTCAGGAC CTCAGAGACC CAGGAGCTAT TGTAATGGCA 
TTTAAAGAAG GGGAGCGTCT GGAGAAAGAA GCCCTCCTCC GCCTCCAGCA GGTTAATTTG 
GCGAAACCCA GTATCGAAAA TGGAGTAAGC ACTGACCAGG ACAGTATCAA GACAGAAAAG 
AAACAGAGTA ACTCCATGGA GACAGATGTG CCCCTTAACT CAGACAGTCC CGTGCTGCAA 
AATGGAGGCT CTTCCCATTC CCTCACCTCT AGCCAAACTG TGTGTATTTG TGGTGGCCCA 
CCTCGCCCCT TGCTTCACCG GTGCCATCTG TGCAAAGACT GGTTCCATGG AGGTTGTGTG 
TCCTTCCCAC CATTGTTGGC CACTTCAGAC ACGCATCTTA CAAAACCCCT CTGCTGGTGG 
GACTGGAACA CGCGTTTTCT CTGTCCGCGG TGCCAGCGAT CCCGACGGCC ACGGCTGGAA 
ACTATTCTAG CGCTGCTGGT GGCGCTGCAG AGGTTACCTG TACGGCTGCC AGAGGGAGAA 
GCGTTACAGT GTCTTACAGA GAGAGCTATT AACTGGCAAG GTCGAGCCAA ACAGGCATTA 
GACACTCCAG AAGTACAGGA GGCGCTAGAC AGACTGCAAC AAGTAGAGGA TGAAATGGTC 
AGTATAAAAG AGGAAGAACC AGAGGAGAAA AAGAAATGGA ATGGAGATGC TGTAATTGTG 
CTTTCTGACT CGGAGGAAGC AGAAGATGGT GTTATCGATC TGACGGAAGA TGGGAACTCG 
CCAAAAAAGA ATGAAAGGAA CGCTGTCAAT GGTACACAGT CTGGCTGTGA AAATGGAATT 
GGCAGGAAGT CGAGTGCACA TGAAGTTACA GGGGTGGAGT CTCTGTTGTC ACTAGTGCCG 
AGTCTTAAAG GGCCAGTGGT GGAGCTGAAC ACATCTGTCA GAGCACAGCT GGAGGAGCTT 
CAGCTTGAAG GAGACCTGCT GGAGGTCACG CTTGACCAGA CACACGCCAT CTACCGCATC 
CTTCAGGCAT CATCTCAGCC GCCCCAGGAC ACCTTGCGGA CACTCATACA GATTGAACTG 
CAGGAGCAGA GAAGCTCAGG TCGTGGCAAC AAGTCGAAAG ACTCCAAGAG GAAGAGAAAG 
AGCCAGAAAA CCGAAGGCGA GCCGAAATCC ACAGAGGCTT CAGACTGCAA GAAGACGAAA 
GCCCTCAATC TCAGCCCACC TACAGACACA CACACAGATG TTGTGATGTT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM5C ( XM_017357332.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212821.1 Danio rerio lysine (K)-specific demethylase 5C (kdm5c), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa41943
Clone ID Related Accession (Same CDS sequence)	XM_017357332.2 , XM_017357332.1
Accession Version	XM_017357332.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 5C isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357332.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATG TAGGGGAGGA GTTTGTTCCA CCACCTGAAT GTCCAGTATT TGAACCATCA 
TGGGAGGAGT TTGCAGATCC ATTGGGATAT ATTGCCAAAA TCCGTCCAAT AGCAGAAAAG 
TCTGGAATAT GCAAGATTCG TCCACCACCC GACTGGCAGC CACCGTTTGC TGTGGAGGTG 
GACAATTTTC ATTTCACACC CCGTATTCAG AGGCTCAATG AGCTAGAGGC TGAGACACGA 
GTAAAGCTGA ATTACCTAGA CAGAATTGCC AAGTTCTGGG AGATCCAGGG ATCCTCTCTG 
AAGATCCCCA ATTTAGAGCG ACGTATTCTG GATCTGTTCA GCTTGGCCAA GATTGTCACA 
GAGGAAGGGG GCTTTGAGTC TGTGAGTAAG GAAAGGCGGT GGGCTCGTGT GGCACAGAAA 
CTCGGTTATC CTCCTGGTAA AAACATTGGC TCTCTCCTGA GGTCCCACTA TGAGAGGATT 
GTGTATCCAT TTGAGTTGTT CCAGTCTGGA GCCAGTTTAC CGCCACGTAA ACCCAGGCAA 
TATGACAGTG ATGAGGTAGA CCGAGAATAC AAACCCCACT CCATTCCTTT GCGTCAGTCA 
GTGCCACCAT CCAAGATGAG CAGCTATGGA CGAAGGGCCA ATCGCCTTCA GCCTGAGCCG 
GAGCCTACAG AAGAAGACAT TGAGAAGAAC CCTGAGCTGA AAAAGCTACA GATCTATGGG 
GCTGGACCTA AAATGATGGG CCTTGGTCTT GTACCTCGAG ACAAGACGCG GAAGAAAGAT 
GATCTGCCGC AGACTGTAAT TGTGCGGGAC AGTGCTGTGA AGGATGAGCC TGCAGAGACG 
GCGGTTACAA AATCAGATCC AGACATTCCA CCGCCACCCC CAAATCTCAT CATCAAGGAG 
GATGTCGATG ATGACAAAAG TCATATTGAC AAACCTAACG ATCCCAGTGA TGAGCCTTGC 
ACTAAGATGA CCATGAGACT CCGACGCAAC CTCAATAATC CACAGTTTGT GGACTCGTTT 
GTGTGTCGAA TGTGTGGACG CGGGGATGAG GACGAGAAGT TGATGCTCTG TGATGGGTGT 
GATGATAATT ACCACACATT CTGCCTGATA CCTCCACTCA CAGACCCACC CAAGGGCAAC 
TGGCGCTGTC CCAAATGCGT AGCAGAGGAG TGTAAAAAGC CATCAGAGGC ATTCGGCTTC 
GAACAGGCCA CAAGAGAGTA CACGTTACAG AGCTTCGGTG AAATGGCCGA CACTTTCAAA 
GCAGACTACT TCAACATGCC TGTCCATATG GTTCCCACAG AGCTGGTTGA AAAGGAGTTT 
TGGAGGTTGG TTAGCAGTAT TGAAGAAGAT GTAACTGTAG AATATGGTGC CGATATTCAC 
TCAAAAGAAT TTGGTAGTGG TTTTCCTGTC AACAACGGCA AGAGACAATT ATCAGAGGAG 
GAAGAGGAAT ATGCCCGCAG CGGCTGGAAC CTAAATGTTA TGCCAGTGTT GGAACAGTCA 
TTGTTGTGCC ACATCAATGC AGATATCTCT GGCATGAAGG TGCCATGGCT ATATGTGGGC 
ATGGTGTTCT CTGCTTTCTG TTGGCACATA GAGGACCACT GGAGTTACTC CATCAATTAT 
TTACACTGGG GCGAGCCCAA AACATGGTAT GGAGTTCCAT GTTCAGCTGC AGAGAAGTTG 
GAGGAGGTGA TGAAGAAACT CACACCTGAG CTATTTGAGT TCCAGCCAGA CCTGCTCCAC 
CAGCTAGTCA CCATCATGAA CCCAAATATA CTCATGAGTC ATGGAGTGCC GGTGGTTCGG 
ACCAATCAGT GTGCTGGGGA GTTTGTCATC ACCTTTCCAC GAGCTTACCA CAGCGGTTTT 
AATCAGGGAT ATAACTTTGC CGAAGCTGTC AACTTCTGCA CTGCAGATTG GTTGCCCACA 
GGGCGCTCTT GTATTGAGCA TTACCGGCAG CTGAGGCGCT ACTGTGTGTT CTCTCAAGAG 
GAACTCACCT GCAAGATGGC GGCCTGCCCA GAAAAGCTGG ATCTGAACCT GGCAGCCGCC 
ACACACAGGG AGATGTTCAT CATAGTGCAG GAAGAGAGGA AACTACGCAA AAGCCTACTG 
GAAAGGGGCA TCAAGGAAGC TGAAAGGGAG GCCTTTGAGT TGCTGCCTGA TGATGAACGA 
CAGTGTGACA AATGTAAGAC CACCTGCTTC CTCTCAGCTC TGGCATGTTC GAACTGCCCA 
GAGCGTCTCG TCTGCCTCTA TCATGCCCAG GACCTCTGCT CTTGCCCGAG TGAAAAACTC 
TACCTCAGGT ATCGCTATAC TCTGGATGAA CTGTTGGCCA TGTTGCATCG GTTGAAAGTT 
CGCGCTGAGT CTTTTGACTC TTGGGCTAAC CGCGTGAAGG AAGCCCTAGA GCAGGATGAA 
GGCAACAAAA TAGATATTAA AGATCTGGAG GTATTAAAAG AAGAGGCAGC TGACAAGAAG 
TTCCCCAATA ATGAGCTGCT CCAGCGCCTC AACACTGTCT TCGTGGACAT AGAGAAGTGC 
GAGAGCTGCA GCACAGAACT CCTCAGCAAC AGCCAGAGCA GTAGAATGAC CCTGAAGGAG 
CTGAAGACTC TGGTGGACAC CATGCAGAAC CTGCCATGTG TTATAAAGCA GCAGGACGAA 
GTTCAGCTGA TATTGCAGAA GATAGAGGAG TTTGATTCCC GTGCTCAAGT TCTGGTCGAC 
AGCACCAAGA GTGAATGGAA GCAGGACTCT CCTCTTCCTG TGGCCACTGA GATCCAGGCT 
CTGCTAGAGG AAGGTGCATC ACTACCGGTT ATTGCCCCAG CTTGTGAGCT TTTGAGTGGG 
CTGTTGGAGC AGGGGCGCTG GCTGGGAGAG GTCAGGCGGA CGTTAGGGCC AGAGGGCAGT 
GAGGTGACTC TGACTGTGCT AAGGAACTTG ATGGAGTCGG GCTGTAATGT ACCACAGAGT 
GTGTCAGTGG AGACTGCAAT GGCTGAACTG CAAGAGCTCT TGACAATAGC TGAACGCTGG 
GAGGAGAAGG CGCAGATATG CTTGGAGGAC AGGCAGAAAC ACCATTTATC AACGCTGGAG 
GCTATTGTGA ATGAGGCTCA ACTAATACCA GTCCAGCTGC CTAATATCCT GTCCCTACAG 
GCCTGCTTGA GCCGAGCTCG GGCTTGGGTC ACAGATCTGG AGGAAATTCA GAATGGTGAG 
CACTATCCAT GTTTGGATGA CCTGGAGGGT CTGGTAGCCA TTGGGAGGGA CTTGCCAGTG 
AAGATGGAGG AGCTGAAGCA GCTGGAGCTG CAGGTGGCCA GTGCTCACTC CTGGAGAGAG 
AAGGCCAGCA AAACCTTTCT GAAGAAGAAC AGCCAACATA GTCTGCTCGA GGTGCTGTGC 
CCATGTGTTG AGAAGAGCAA GAAGATGGAG GACAACTCAA TGGTTACAGA AGCAGATTCG 
GATGTCAATG TATTAGGCCT CACAGCTCAG GACCTCAGAG ACCCAGGAGC TATTGTAATG 
GCATTTAAAG AAGGGGAGCG TCTGGAGAAA GAAGCCCTCC TCCGCCTCCA GCAGGTTAAT 
TTGGCGAAAC CCAGTATCGA AAATGGAGTA AGCACTGACC AGGACAGTAT CAAGACAGAA 
AAGAAACAGA GTAACTCCAT GGAGACAGAT GTGCCCCTTA ACTCAGACAG TCCCGTGCTG 
CAAAATGGAG GCTCTTCCCA TTCCCTCACC TCTAGCCAAA CTGTGTGTAT TTGTGGTGGC 
CCACCTCGCC CCTTGCTTCA CCGGTGCCAT CTGTGCAAAG ACTGGTTCCA TGGAGGTTGT 
GTGTCCTTCC CACCATTGTT GGCCACTTCA GACACGCATC TTACAAAACC CCTCTGCTGG 
TGGGACTGGA ACACGCGTTT TCTCTGTCCG CGGTGCCAGC GATCCCGACG GCCACGGCTG 
GAAACTATTC TAGCGCTGCT GGTGGCGCTG CAGAGGTTAC CTGTACGGCT GCCAGAGGGA 
GAAGCGTTAC AGTGTCTTAC AGAGAGAGCT ATTAACTGGC AAGGTCGAGC CAAACAGGCA 
TTAGACACTC CAGAAGTACA GGAGGCGCTA GACAGACTGC AACAAGTAGA GGATGAAATG 
GTCAGTATAA AAGAGGAAGA ACCAGAGGAG AAAAAGAAAT GGAATGGAGA TGCTGTAATT 
GTGCTTTCTG ACTCGGAGGA AGCAGAAGAT GGTGTTATCG ATCTGACGGA AGATGGGAAC 
TCGCCAAAAA AGAATGAAAG GAACGCTGTC AATGGTACAC AGTCTGGCTG TGAAAATGGA 
ATTGGCAGGA AGTCGAGTGC ACATGAAGTT ACAGGGGTGG AGTCTCTGTT GTCACTAGTG 
CCGAGTCTTA AAGGGCCAGT GGTGGAGCTG AACACATCTG TCAGAGCACA GCTGGAGGAG 
CTTCAGCTTG AAGGAGACCT GCTGGAGGTC ACGCTTGACC AGACACACGC CATCTACCGC 
ATCCTTCAGG CATCATCTCA GCCGCCCCAG GACACCTTGC GGACACTCAT ACAGATTGAA 
CTGCAGGAGC AGAGAAGCTC AGGTCGTGGC AACAAGTCGA AAGACTCCAA GAGGAAGAGA 
AAGAGCCAGA AAACCGAAGG CGAGCCGAAA TCCACAGAGG CTTCAGACTG CAAGAAGACG 
AAAGCCCTCA ATCTCAGCCC ACCTACAGAC ACACACACAG ATGTTGTGAT GTTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212821.1
CDS	462..5141
Translation	MMDVGEEFVPPPECPVFEPSWEEFADPLGYIAKIRPIAEKSGICKIRPPPDWQPPFAVEVDNFHFTPRIQRLNELEAETRVKLNYLDRIAKFWEIQGSSLKIPNLERRILDLFSLAKIVTEEGGFESVSKERRWARVAQKLGYPPGKNIGSLLRSHYERIVYPFELFQSGASLPPRKPRQYDSDEVDREYKPHSIPLRQSVPPSKMSSYGRRANRLQPEPEPTEEDIEKNPELKKLQIYGAGPKMMGLGLVPRDKTRKKDDLPQTVIVRDSAVKDEPAETAVTKSDPDIPPPPPNLIIKEDVDDDKSHIDKPNDPSDEPCTKMTMRLRRNLNNPQFVDSFVCRMCGRGDEDEKLMLCDGCDDNYHTFCLIPPLTDPPKGNWRCPKCVAEECKKPSEAFGFEQATREYTLQSFGEMADTFKADYFNMPVHMVPTELVEKEFWRLVSSIEEDVTVEYGADIHSKEFGSGFPVNNGKRQLSEEEEEYARSGWNLNVMPVLEQSLLCHINADISGMKVPWLYVGMVFSAFCWHIEDHWSYSINYLHWGEPKTWYGVPCSAAEKLEEVMKKLTPELFEFQPDLLHQLVTIMNPNILMSHGVPVVRTNQCAGEFVITFPRAYHSGFNQGYNFAEAVNFCTADWLPTGRSCIEHYRQLRRYCVFSQEELTCKMAACPEKLDLNLAAATHREMFIIVQEERKLRKSLLERGIKEAEREAFELLPDDERQCDKCKTTCFLSALACSNCPERLVCLYHAQDLCSCPSEKLYLRYRYTLDELLAMLHRLKVRAESFDSWANRVKEALEQDEGNKIDIKDLEVLKEEAADKKFPNNELLQRLNTVFVDIEKCESCSTELLSNSQSSRMTLKELKTLVDTMQNLPCVIKQQDEVQLILQKIEEFDSRAQVLVDSTKSEWKQDSPLPVATEIQALLEEGASLPVIAPACELLSGLLEQGRWLGEVRRTLGPEGSEVTLTVLRNLMESGCNVPQSVSVETAMAELQELLTIAERWEEKAQICLEDRQKHHLSTLEAIVNEAQLIPVQLPNILSLQACLSRARAWVTDLEEIQNGEHYPCLDDLEGLVAIGRDLPVKMEELKQLELQVASAHSWREKASKTFLKKNSQHSLLEVLCPCVEKSKKMEDNSMVTEADSDVNVLGLTAQDLRDPGAIVMAFKEGERLEKEALLRLQQVNLAKPSIENGVSTDQDSIKTEKKQSNSMETDVPLNSDSPVLQNGGSSHSLTSSQTVCICGGPPRPLLHRCHLCKDWFHGGCVSFPPLLATSDTHLTKPLCWWDWNTRFLCPRCQRSRRPRLETILALLVALQRLPVRLPEGEALQCLTERAINWQGRAKQALDTPEVQEALDRLQQVEDEMVSIKEEEPEEKKKWNGDAVIVLSDSEEAEDGVIDLTEDGNSPKKNERNAVNGTQSGCENGIGRKSSAHEVTGVESLLSLVPSLKGPVVELNTSVRAQLEELQLEGDLLEVTLDQTHAIYRILQASSQPPQDTLRTLIQIELQEQRSSGRGNKSKDSKRKRKSQKTEGEPKSTEASDCKKTKALNLSPPTDTHTDVVMFS

Target ORF information:

RefSeq Version	XM_017357332.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 5C (kdm5c), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357332.2

ORF Insert Sequence:

ATGATGGATG TAGGGGAGGA GTTTGTTCCA CCACCTGAAT GTCCAGTATT TGAACCATCA 
TGGGAGGAGT TTGCAGATCC ATTGGGATAT ATTGCCAAAA TCCGTCCAAT AGCAGAAAAG 
TCTGGAATAT GCAAGATTCG TCCACCACCC GACTGGCAGC CACCGTTTGC TGTGGAGGTG 
GACAATTTTC ATTTCACACC CCGTATTCAG AGGCTCAATG AGCTAGAGGC TGAGACACGA 
GTAAAGCTGA ATTACCTAGA CAGAATTGCC AAGTTCTGGG AGATCCAGGG ATCCTCTCTG 
AAGATCCCCA ATTTAGAGCG ACGTATTCTG GATCTGTTCA GCTTGGCCAA GATTGTCACA 
GAGGAAGGGG GCTTTGAGTC TGTGAGTAAG GAAAGGCGGT GGGCTCGTGT GGCACAGAAA 
CTCGGTTATC CTCCTGGTAA AAACATTGGC TCTCTCCTGA GGTCCCACTA TGAGAGGATT 
GTGTATCCAT TTGAGTTGTT CCAGTCTGGA GCCAGTTTAC CGCCACGTAA ACCCAGGCAA 
TATGACAGTG ATGAGGTAGA CCGAGAATAC AAACCCCACT CCATTCCTTT GCGTCAGTCA 
GTGCCACCAT CCAAGATGAG CAGCTATGGA CGAAGGGCCA ATCGCCTTCA GCCTGAGCCG 
GAGCCTACAG AAGAAGACAT TGAGAAGAAC CCTGAGCTGA AAAAGCTACA GATCTATGGG 
GCTGGACCTA AAATGATGGG CCTTGGTCTT GTACCTCGAG ACAAGACGCG GAAGAAAGAT 
GATCTGCCGC AGACTGTAAT TGTGCGGGAC AGTGCTGTGA AGGATGAGCC TGCAGAGACG 
GCGGTTACAA AATCAGATCC AGACATTCCA CCGCCACCCC CAAATCTCAT CATCAAGGAG 
GATGTCGATG ATGACAAAAG TCATATTGAC AAACCTAACG ATCCCAGTGA TGAGCCTTGC 
ACTAAGATGA CCATGAGACT CCGACGCAAC CTCAATAATC CACAGTTTGT GGACTCGTTT 
GTGTGTCGAA TGTGTGGACG CGGGGATGAG GACGAGAAGT TGATGCTCTG TGATGGGTGT 
GATGATAATT ACCACACATT CTGCCTGATA CCTCCACTCA CAGACCCACC CAAGGGCAAC 
TGGCGCTGTC CCAAATGCGT AGCAGAGGAG TGTAAAAAGC CATCAGAGGC ATTCGGCTTC 
GAACAGGCCA CAAGAGAGTA CACGTTACAG AGCTTCGGTG AAATGGCCGA CACTTTCAAA 
GCAGACTACT TCAACATGCC TGTCCATATG GTTCCCACAG AGCTGGTTGA AAAGGAGTTT 
TGGAGGTTGG TTAGCAGTAT TGAAGAAGAT GTAACTGTAG AATATGGTGC CGATATTCAC 
TCAAAAGAAT TTGGTAGTGG TTTTCCTGTC AACAACGGCA AGAGACAATT ATCAGAGGAG 
GAAGAGGAAT ATGCCCGCAG CGGCTGGAAC CTAAATGTTA TGCCAGTGTT GGAACAGTCA 
TTGTTGTGCC ACATCAATGC AGATATCTCT GGCATGAAGG TGCCATGGCT ATATGTGGGC 
ATGGTGTTCT CTGCTTTCTG TTGGCACATA GAGGACCACT GGAGTTACTC CATCAATTAT 
TTACACTGGG GCGAGCCCAA AACATGGTAT GGAGTTCCAT GTTCAGCTGC AGAGAAGTTG 
GAGGAGGTGA TGAAGAAACT CACACCTGAG CTATTTGAGT TCCAGCCAGA CCTGCTCCAC 
CAGCTAGTCA CCATCATGAA CCCAAATATA CTCATGAGTC ATGGAGTGCC GGTGGTTCGG 
ACCAATCAGT GTGCTGGGGA GTTTGTCATC ACCTTTCCAC GAGCTTACCA CAGCGGTTTT 
AATCAGGGAT ATAACTTTGC CGAAGCTGTC AACTTCTGCA CTGCAGATTG GTTGCCCACA 
GGGCGCTCTT GTATTGAGCA TTACCGGCAG CTGAGGCGCT ACTGTGTGTT CTCTCAAGAG 
GAACTCACCT GCAAGATGGC GGCCTGCCCA GAAAAGCTGG ATCTGAACCT GGCAGCCGCC 
ACACACAGGG AGATGTTCAT CATAGTGCAG GAAGAGAGGA AACTACGCAA AAGCCTACTG 
GAAAGGGGCA TCAAGGAAGC TGAAAGGGAG GCCTTTGAGT TGCTGCCTGA TGATGAACGA 
CAGTGTGACA AATGTAAGAC CACCTGCTTC CTCTCAGCTC TGGCATGTTC GAACTGCCCA 
GAGCGTCTCG TCTGCCTCTA TCATGCCCAG GACCTCTGCT CTTGCCCGAG TGAAAAACTC 
TACCTCAGGT ATCGCTATAC TCTGGATGAA CTGTTGGCCA TGTTGCATCG GTTGAAAGTT 
CGCGCTGAGT CTTTTGACTC TTGGGCTAAC CGCGTGAAGG AAGCCCTAGA GCAGGATGAA 
GGCAACAAAA TAGATATTAA AGATCTGGAG GTATTAAAAG AAGAGGCAGC TGACAAGAAG 
TTCCCCAATA ATGAGCTGCT CCAGCGCCTC AACACTGTCT TCGTGGACAT AGAGAAGTGC 
GAGAGCTGCA GCACAGAACT CCTCAGCAAC AGCCAGAGCA GTAGAATGAC CCTGAAGGAG 
CTGAAGACTC TGGTGGACAC CATGCAGAAC CTGCCATGTG TTATAAAGCA GCAGGACGAA 
GTTCAGCTGA TATTGCAGAA GATAGAGGAG TTTGATTCCC GTGCTCAAGT TCTGGTCGAC 
AGCACCAAGA GTGAATGGAA GCAGGACTCT CCTCTTCCTG TGGCCACTGA GATCCAGGCT 
CTGCTAGAGG AAGGTGCATC ACTACCGGTT ATTGCCCCAG CTTGTGAGCT TTTGAGTGGG 
CTGTTGGAGC AGGGGCGCTG GCTGGGAGAG GTCAGGCGGA CGTTAGGGCC AGAGGGCAGT 
GAGGTGACTC TGACTGTGCT AAGGAACTTG ATGGAGTCGG GCTGTAATGT ACCACAGAGT 
GTGTCAGTGG AGACTGCAAT GGCTGAACTG CAAGAGCTCT TGACAATAGC TGAACGCTGG 
GAGGAGAAGG CGCAGATATG CTTGGAGGAC AGGCAGAAAC ACCATTTATC AACGCTGGAG 
GCTATTGTGA ATGAGGCTCA ACTAATACCA GTCCAGCTGC CTAATATCCT GTCCCTACAG 
GCCTGCTTGA GCCGAGCTCG GGCTTGGGTC ACAGATCTGG AGGAAATTCA GAATGGTGAG 
CACTATCCAT GTTTGGATGA CCTGGAGGGT CTGGTAGCCA TTGGGAGGGA CTTGCCAGTG 
AAGATGGAGG AGCTGAAGCA GCTGGAGCTG CAGGTGGCCA GTGCTCACTC CTGGAGAGAG 
AAGGCCAGCA AAACCTTTCT GAAGAAGAAC AGCCAACATA GTCTGCTCGA GGTGCTGTGC 
CCATGTGTTG AGAAGAGCAA GAAGATGGAG GACAACTCAA TGGTTACAGA AGCAGATTCG 
GATGTCAATG TATTAGGCCT CACAGCTCAG GACCTCAGAG ACCCAGGAGC TATTGTAATG 
GCATTTAAAG AAGGGGAGCG TCTGGAGAAA GAAGCCCTCC TCCGCCTCCA GCAGGTTAAT 
TTGGCGAAAC CCAGTATCGA AAATGGAGTA AGCACTGACC AGGACAGTAT CAAGACAGAA 
AAGAAACAGA GTAACTCCAT GGAGACAGAT GTGCCCCTTA ACTCAGACAG TCCCGTGCTG 
CAAAATGGAG GCTCTTCCCA TTCCCTCACC TCTAGCCAAA CTGTGTGTAT TTGTGGTGGC 
CCACCTCGCC CCTTGCTTCA CCGGTGCCAT CTGTGCAAAG ACTGGTTCCA TGGAGGTTGT 
GTGTCCTTCC CACCATTGTT GGCCACTTCA GACACGCATC TTACAAAACC CCTCTGCTGG 
TGGGACTGGA ACACGCGTTT TCTCTGTCCG CGGTGCCAGC GATCCCGACG GCCACGGCTG 
GAAACTATTC TAGCGCTGCT GGTGGCGCTG CAGAGGTTAC CTGTACGGCT GCCAGAGGGA 
GAAGCGTTAC AGTGTCTTAC AGAGAGAGCT ATTAACTGGC AAGGTCGAGC CAAACAGGCA 
TTAGACACTC CAGAAGTACA GGAGGCGCTA GACAGACTGC AACAAGTAGA GGATGAAATG 
GTCAGTATAA AAGAGGAAGA ACCAGAGGAG AAAAAGAAAT GGAATGGAGA TGCTGTAATT 
GTGCTTTCTG ACTCGGAGGA AGCAGAAGAT GGTGTTATCG ATCTGACGGA AGATGGGAAC 
TCGCCAAAAA AGAATGAAAG GAACGCTGTC AATGGTACAC AGTCTGGCTG TGAAAATGGA 
ATTGGCAGGA AGTCGAGTGC ACATGAAGTT ACAGGGGTGG AGTCTCTGTT GTCACTAGTG 
CCGAGTCTTA AAGGGCCAGT GGTGGAGCTG AACACATCTG TCAGAGCACA GCTGGAGGAG 
CTTCAGCTTG AAGGAGACCT GCTGGAGGTC ACGCTTGACC AGACACACGC CATCTACCGC 
ATCCTTCAGG CATCATCTCA GCCGCCCCAG GACACCTTGC GGACACTCAT ACAGATTGAA 
CTGCAGGAGC AGAGAAGCTC AGGTCGTGGC AACAAGTCGA AAGACTCCAA GAGGAAGAGA 
AAGAGCCAGA AAACCGAAGG CGAGCCGAAA TCCACAGAGG CTTCAGACTG CAAGAAGACG 
AAAGCCCTCA ATCTCAGCCC ACCTACAGAC ACACACACAG ATGTTGTGAT GTTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM5C ( XM_017357332.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212821.1 Danio rerio lysine (K)-specific demethylase 5C (kdm5c), transcript variant X1, mRNA.

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CloneID	ODa41943
Clone ID Related Accession (Same CDS sequence)	XM_017357332.2 , XM_017357332.1
Accession Version	XM_017357332.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	lysine-specific demethylase 5C isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATG TAGGGGAGGA GTTTGTTCCA CCACCTGAAT GTCCAGTATT TGAACCATCA 
TGGGAGGAGT TTGCAGATCC ATTGGGATAT ATTGCCAAAA TCCGTCCAAT AGCAGAAAAG 
TCTGGAATAT GCAAGATTCG TCCACCACCC GACTGGCAGC CACCGTTTGC TGTGGAGGTG 
GACAATTTTC ATTTCACACC CCGTATTCAG AGGCTCAATG AGCTAGAGGC TGAGACACGA 
GTAAAGCTGA ATTACCTAGA CAGAATTGCC AAGTTCTGGG AGATCCAGGG ATCCTCTCTG 
AAGATCCCCA ATTTAGAGCG ACGTATTCTG GATCTGTTCA GCTTGGCCAA GATTGTCACA 
GAGGAAGGGG GCTTTGAGTC TGTGAGTAAG GAAAGGCGGT GGGCTCGTGT GGCACAGAAA 
CTCGGTTATC CTCCTGGTAA AAACATTGGC TCTCTCCTGA GGTCCCACTA TGAGAGGATT 
GTGTATCCAT TTGAGTTGTT CCAGTCTGGA GCCAGTTTAC CGCCACGTAA ACCCAGGCAA 
TATGACAGTG ATGAGGTAGA CCGAGAATAC AAACCCCACT CCATTCCTTT GCGTCAGTCA 
GTGCCACCAT CCAAGATGAG CAGCTATGGA CGAAGGGCCA ATCGCCTTCA GCCTGAGCCG 
GAGCCTACAG AAGAAGACAT TGAGAAGAAC CCTGAGCTGA AAAAGCTACA GATCTATGGG 
GCTGGACCTA AAATGATGGG CCTTGGTCTT GTACCTCGAG ACAAGACGCG GAAGAAAGAT 
GATCTGCCGC AGACTGTAAT TGTGCGGGAC AGTGCTGTGA AGGATGAGCC TGCAGAGACG 
GCGGTTACAA AATCAGATCC AGACATTCCA CCGCCACCCC CAAATCTCAT CATCAAGGAG 
GATGTCGATG ATGACAAAAG TCATATTGAC AAACCTAACG ATCCCAGTGA TGAGCCTTGC 
ACTAAGATGA CCATGAGACT CCGACGCAAC CTCAATAATC CACAGTTTGT GGACTCGTTT 
GTGTGTCGAA TGTGTGGACG CGGGGATGAG GACGAGAAGT TGATGCTCTG TGATGGGTGT 
GATGATAATT ACCACACATT CTGCCTGATA CCTCCACTCA CAGACCCACC CAAGGGCAAC 
TGGCGCTGTC CCAAATGCGT AGCAGAGGAG TGTAAAAAGC CATCAGAGGC ATTCGGCTTC 
GAACAGGCCA CAAGAGAGTA CACGTTACAG AGCTTCGGTG AAATGGCCGA CACTTTCAAA 
GCAGACTACT TCAACATGCC TGTCCATATG GTTCCCACAG AGCTGGTTGA AAAGGAGTTT 
TGGAGGTTGG TTAGCAGTAT TGAAGAAGAT GTAACTGTAG AATATGGTGC CGATATTCAC 
TCAAAAGAAT TTGGTAGTGG TTTTCCTGTC AACAACGGCA AGAGACAATT ATCAGAGGAG 
GAAGAGGAAT ATGCCCGCAG CGGCTGGAAC CTAAATGTTA TGCCAGTGTT GGAACAGTCA 
TTGTTGTGCC ACATCAATGC AGATATCTCT GGCATGAAGG TGCCATGGCT ATATGTGGGC 
ATGGTGTTCT CTGCTTTCTG TTGGCACATA GAGGACCACT GGAGTTACTC CATCAATTAT 
TTACACTGGG GCGAGCCCAA AACATGGTAT GGAGTTCCAT GTTCAGCTGC AGAGAAGTTG 
GAGGAGGTGA TGAAGAAACT CACACCTGAG CTATTTGAGT TCCAGCCAGA CCTGCTCCAC 
CAGCTAGTCA CCATCATGAA CCCAAATATA CTCATGAGTC ATGGAGTGCC GGTGGTTCGG 
ACCAATCAGT GTGCTGGGGA GTTTGTCATC ACCTTTCCAC GAGCTTACCA CAGCGGTTTT 
AATCAGGGAT ATAACTTTGC CGAAGCTGTC AACTTCTGCA CTGCAGATTG GTTGCCCACA 
GGGCGCTCTT GTATTGAGCA TTACCGGCAG CTGAGGCGCT ACTGTGTGTT CTCTCAAGAG 
GAACTCACCT GCAAGATGGC GGCCTGCCCA GAAAAGCTGG ATCTGAACCT GGCAGCCGCC 
ACACACAGGG AGATGTTCAT CATAGTGCAG GAAGAGAGGA AACTACGCAA AAGCCTACTG 
GAAAGGGGCA TCAAGGAAGC TGAAAGGGAG GCCTTTGAGT TGCTGCCTGA TGATGAACGA 
CAGTGTGACA AATGTAAGAC CACCTGCTTC CTCTCAGCTC TGGCATGTTC GAACTGCCCA 
GAGCGTCTCG TCTGCCTCTA TCATGCCCAG GACCTCTGCT CTTGCCCGAG TGAAAAACTC 
TACCTCAGGT ATCGCTATAC TCTGGATGAA CTGTTGGCCA TGTTGCATCG GTTGAAAGTT 
CGCGCTGAGT CTTTTGACTC TTGGGCTAAC CGCGTGAAGG AAGCCCTAGA GCAGGATGAA 
GGCAACAAAA TAGATATTAA AGATCTGGAG GTATTAAAAG AAGAGGCAGC TGACAAGAAG 
TTCCCCAATA ATGAGCTGCT CCAGCGCCTC AACACTGTCT TCGTGGACAT AGAGAAGTGC 
GAGAGCTGCA GCACAGAACT CCTCAGCAAC AGCCAGAGCA GTAGAATGAC CCTGAAGGAG 
CTGAAGACTC TGGTGGACAC CATGCAGAAC CTGCCATGTG TTATAAAGCA GCAGGACGAA 
GTTCAGCTGA TATTGCAGAA GATAGAGGAG TTTGATTCCC GTGCTCAAGT TCTGGTCGAC 
AGCACCAAGA GTGAATGGAA GCAGGACTCT CCTCTTCCTG TGGCCACTGA GATCCAGGCT 
CTGCTAGAGG AAGGTGCATC ACTACCGGTT ATTGCCCCAG CTTGTGAGCT TTTGAGTGGG 
CTGTTGGAGC AGGGGCGCTG GCTGGGAGAG GTCAGGCGGA CGTTAGGGCC AGAGGGCAGT 
GAGGTGACTC TGACTGTGCT AAGGAACTTG ATGGAGTCGG GCTGTAATGT ACCACAGAGT 
GTGTCAGTGG AGACTGCAAT GGCTGAACTG CAAGAGCTCT TGACAATAGC TGAACGCTGG 
GAGGAGAAGG CGCAGATATG CTTGGAGGAC AGGCAGAAAC ACCATTTATC AACGCTGGAG 
GCTATTGTGA ATGAGGCTCA ACTAATACCA GTCCAGCTGC CTAATATCCT GTCCCTACAG 
GCCTGCTTGA GCCGAGCTCG GGCTTGGGTC ACAGATCTGG AGGAAATTCA GAATGGTGAG 
CACTATCCAT GTTTGGATGA CCTGGAGGGT CTGGTAGCCA TTGGGAGGGA CTTGCCAGTG 
AAGATGGAGG AGCTGAAGCA GCTGGAGCTG CAGGTGGCCA GTGCTCACTC CTGGAGAGAG 
AAGGCCAGCA AAACCTTTCT GAAGAAGAAC AGCCAACATA GTCTGCTCGA GGTGCTGTGC 
CCATGTGTTG AGAAGAGCAA GAAGATGGAG GACAACTCAA TGGTTACAGA AGCAGATTCG 
GATGTCAATG TATTAGGCCT CACAGCTCAG GACCTCAGAG ACCCAGGAGC TATTGTAATG 
GCATTTAAAG AAGGGGAGCG TCTGGAGAAA GAAGCCCTCC TCCGCCTCCA GCAGGTTAAT 
TTGGCGAAAC CCAGTATCGA AAATGGAGTA AGCACTGACC AGGACAGTAT CAAGACAGAA 
AAGAAACAGA GTAACTCCAT GGAGACAGAT GTGCCCCTTA ACTCAGACAG TCCCGTGCTG 
CAAAATGGAG GCTCTTCCCA TTCCCTCACC TCTAGCCAAA CTGTGTGTAT TTGTGGTGGC 
CCACCTCGCC CCTTGCTTCA CCGGTGCCAT CTGTGCAAAG ACTGGTTCCA TGGAGGTTGT 
GTGTCCTTCC CACCATTGTT GGCCACTTCA GACACGCATC TTACAAAACC CCTCTGCTGG 
TGGGACTGGA ACACGCGTTT TCTCTGTCCG CGGTGCCAGC GATCCCGACG GCCACGGCTG 
GAAACTATTC TAGCGCTGCT GGTGGCGCTG CAGAGGTTAC CTGTACGGCT GCCAGAGGGA 
GAAGCGTTAC AGTGTCTTAC AGAGAGAGCT ATTAACTGGC AAGGTCGAGC CAAACAGGCA 
TTAGACACTC CAGAAGTACA GGAGGCGCTA GACAGACTGC AACAAGTAGA GGATGAAATG 
GTCAGTATAA AAGAGGAAGA ACCAGAGGAG AAAAAGAAAT GGAATGGAGA TGCTGTAATT 
GTGCTTTCTG ACTCGGAGGA AGCAGAAGAT GGTGTTATCG ATCTGACGGA AGATGGGAAC 
TCGCCAAAAA AGAATGAAAG GAACGCTGTC AATGGTACAC AGTCTGGCTG TGAAAATGGA 
ATTGGCAGGA AGTCGAGTGC ACATGAAGTT ACAGGGGTGG AGTCTCTGTT GTCACTAGTG 
CCGAGTCTTA AAGGGCCAGT GGTGGAGCTG AACACATCTG TCAGAGCACA GCTGGAGGAG 
CTTCAGCTTG AAGGAGACCT GCTGGAGGTC ACGCTTGACC AGACACACGC CATCTACCGC 
ATCCTTCAGG CATCATCTCA GCCGCCCCAG GACACCTTGC GGACACTCAT ACAGATTGAA 
CTGCAGGAGC AGAGAAGCTC AGGTCGTGGC AACAAGTCGA AAGACTCCAA GAGGAAGAGA 
AAGAGCCAGA AAACCGAAGG CGAGCCGAAA TCCACAGAGG CTTCAGACTG CAAGAAGACG 
AAAGCCCTCA ATCTCAGCCC ACCTACAGAC ACACACACAG ATGTTGTGAT GTTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212821.1
CDS	467..5146
Translation	MMDVGEEFVPPPECPVFEPSWEEFADPLGYIAKIRPIAEKSGICKIRPPPDWQPPFAVEVDNFHFTPRIQRLNELEAETRVKLNYLDRIAKFWEIQGSSLKIPNLERRILDLFSLAKIVTEEGGFESVSKERRWARVAQKLGYPPGKNIGSLLRSHYERIVYPFELFQSGASLPPRKPRQYDSDEVDREYKPHSIPLRQSVPPSKMSSYGRRANRLQPEPEPTEEDIEKNPELKKLQIYGAGPKMMGLGLVPRDKTRKKDDLPQTVIVRDSAVKDEPAETAVTKSDPDIPPPPPNLIIKEDVDDDKSHIDKPNDPSDEPCTKMTMRLRRNLNNPQFVDSFVCRMCGRGDEDEKLMLCDGCDDNYHTFCLIPPLTDPPKGNWRCPKCVAEECKKPSEAFGFEQATREYTLQSFGEMADTFKADYFNMPVHMVPTELVEKEFWRLVSSIEEDVTVEYGADIHSKEFGSGFPVNNGKRQLSEEEEEYARSGWNLNVMPVLEQSLLCHINADISGMKVPWLYVGMVFSAFCWHIEDHWSYSINYLHWGEPKTWYGVPCSAAEKLEEVMKKLTPELFEFQPDLLHQLVTIMNPNILMSHGVPVVRTNQCAGEFVITFPRAYHSGFNQGYNFAEAVNFCTADWLPTGRSCIEHYRQLRRYCVFSQEELTCKMAACPEKLDLNLAAATHREMFIIVQEERKLRKSLLERGIKEAEREAFELLPDDERQCDKCKTTCFLSALACSNCPERLVCLYHAQDLCSCPSEKLYLRYRYTLDELLAMLHRLKVRAESFDSWANRVKEALEQDEGNKIDIKDLEVLKEEAADKKFPNNELLQRLNTVFVDIEKCESCSTELLSNSQSSRMTLKELKTLVDTMQNLPCVIKQQDEVQLILQKIEEFDSRAQVLVDSTKSEWKQDSPLPVATEIQALLEEGASLPVIAPACELLSGLLEQGRWLGEVRRTLGPEGSEVTLTVLRNLMESGCNVPQSVSVETAMAELQELLTIAERWEEKAQICLEDRQKHHLSTLEAIVNEAQLIPVQLPNILSLQACLSRARAWVTDLEEIQNGEHYPCLDDLEGLVAIGRDLPVKMEELKQLELQVASAHSWREKASKTFLKKNSQHSLLEVLCPCVEKSKKMEDNSMVTEADSDVNVLGLTAQDLRDPGAIVMAFKEGERLEKEALLRLQQVNLAKPSIENGVSTDQDSIKTEKKQSNSMETDVPLNSDSPVLQNGGSSHSLTSSQTVCICGGPPRPLLHRCHLCKDWFHGGCVSFPPLLATSDTHLTKPLCWWDWNTRFLCPRCQRSRRPRLETILALLVALQRLPVRLPEGEALQCLTERAINWQGRAKQALDTPEVQEALDRLQQVEDEMVSIKEEEPEEKKKWNGDAVIVLSDSEEAEDGVIDLTEDGNSPKKNERNAVNGTQSGCENGIGRKSSAHEVTGVESLLSLVPSLKGPVVELNTSVRAQLEELQLEGDLLEVTLDQTHAIYRILQASSQPPQDTLRTLIQIELQEQRSSGRGNKSKDSKRKRKSQKTEGEPKSTEASDCKKTKALNLSPPTDTHTDVVMFS

Target ORF information:

RefSeq Version	XM_017357332.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 5C (kdm5c), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357332.1

ORF Insert Sequence:

ATGATGGATG TAGGGGAGGA GTTTGTTCCA CCACCTGAAT GTCCAGTATT TGAACCATCA 
TGGGAGGAGT TTGCAGATCC ATTGGGATAT ATTGCCAAAA TCCGTCCAAT AGCAGAAAAG 
TCTGGAATAT GCAAGATTCG TCCACCACCC GACTGGCAGC CACCGTTTGC TGTGGAGGTG 
GACAATTTTC ATTTCACACC CCGTATTCAG AGGCTCAATG AGCTAGAGGC TGAGACACGA 
GTAAAGCTGA ATTACCTAGA CAGAATTGCC AAGTTCTGGG AGATCCAGGG ATCCTCTCTG 
AAGATCCCCA ATTTAGAGCG ACGTATTCTG GATCTGTTCA GCTTGGCCAA GATTGTCACA 
GAGGAAGGGG GCTTTGAGTC TGTGAGTAAG GAAAGGCGGT GGGCTCGTGT GGCACAGAAA 
CTCGGTTATC CTCCTGGTAA AAACATTGGC TCTCTCCTGA GGTCCCACTA TGAGAGGATT 
GTGTATCCAT TTGAGTTGTT CCAGTCTGGA GCCAGTTTAC CGCCACGTAA ACCCAGGCAA 
TATGACAGTG ATGAGGTAGA CCGAGAATAC AAACCCCACT CCATTCCTTT GCGTCAGTCA 
GTGCCACCAT CCAAGATGAG CAGCTATGGA CGAAGGGCCA ATCGCCTTCA GCCTGAGCCG 
GAGCCTACAG AAGAAGACAT TGAGAAGAAC CCTGAGCTGA AAAAGCTACA GATCTATGGG 
GCTGGACCTA AAATGATGGG CCTTGGTCTT GTACCTCGAG ACAAGACGCG GAAGAAAGAT 
GATCTGCCGC AGACTGTAAT TGTGCGGGAC AGTGCTGTGA AGGATGAGCC TGCAGAGACG 
GCGGTTACAA AATCAGATCC AGACATTCCA CCGCCACCCC CAAATCTCAT CATCAAGGAG 
GATGTCGATG ATGACAAAAG TCATATTGAC AAACCTAACG ATCCCAGTGA TGAGCCTTGC 
ACTAAGATGA CCATGAGACT CCGACGCAAC CTCAATAATC CACAGTTTGT GGACTCGTTT 
GTGTGTCGAA TGTGTGGACG CGGGGATGAG GACGAGAAGT TGATGCTCTG TGATGGGTGT 
GATGATAATT ACCACACATT CTGCCTGATA CCTCCACTCA CAGACCCACC CAAGGGCAAC 
TGGCGCTGTC CCAAATGCGT AGCAGAGGAG TGTAAAAAGC CATCAGAGGC ATTCGGCTTC 
GAACAGGCCA CAAGAGAGTA CACGTTACAG AGCTTCGGTG AAATGGCCGA CACTTTCAAA 
GCAGACTACT TCAACATGCC TGTCCATATG GTTCCCACAG AGCTGGTTGA AAAGGAGTTT 
TGGAGGTTGG TTAGCAGTAT TGAAGAAGAT GTAACTGTAG AATATGGTGC CGATATTCAC 
TCAAAAGAAT TTGGTAGTGG TTTTCCTGTC AACAACGGCA AGAGACAATT ATCAGAGGAG 
GAAGAGGAAT ATGCCCGCAG CGGCTGGAAC CTAAATGTTA TGCCAGTGTT GGAACAGTCA 
TTGTTGTGCC ACATCAATGC AGATATCTCT GGCATGAAGG TGCCATGGCT ATATGTGGGC 
ATGGTGTTCT CTGCTTTCTG TTGGCACATA GAGGACCACT GGAGTTACTC CATCAATTAT 
TTACACTGGG GCGAGCCCAA AACATGGTAT GGAGTTCCAT GTTCAGCTGC AGAGAAGTTG 
GAGGAGGTGA TGAAGAAACT CACACCTGAG CTATTTGAGT TCCAGCCAGA CCTGCTCCAC 
CAGCTAGTCA CCATCATGAA CCCAAATATA CTCATGAGTC ATGGAGTGCC GGTGGTTCGG 
ACCAATCAGT GTGCTGGGGA GTTTGTCATC ACCTTTCCAC GAGCTTACCA CAGCGGTTTT 
AATCAGGGAT ATAACTTTGC CGAAGCTGTC AACTTCTGCA CTGCAGATTG GTTGCCCACA 
GGGCGCTCTT GTATTGAGCA TTACCGGCAG CTGAGGCGCT ACTGTGTGTT CTCTCAAGAG 
GAACTCACCT GCAAGATGGC GGCCTGCCCA GAAAAGCTGG ATCTGAACCT GGCAGCCGCC 
ACACACAGGG AGATGTTCAT CATAGTGCAG GAAGAGAGGA AACTACGCAA AAGCCTACTG 
GAAAGGGGCA TCAAGGAAGC TGAAAGGGAG GCCTTTGAGT TGCTGCCTGA TGATGAACGA 
CAGTGTGACA AATGTAAGAC CACCTGCTTC CTCTCAGCTC TGGCATGTTC GAACTGCCCA 
GAGCGTCTCG TCTGCCTCTA TCATGCCCAG GACCTCTGCT CTTGCCCGAG TGAAAAACTC 
TACCTCAGGT ATCGCTATAC TCTGGATGAA CTGTTGGCCA TGTTGCATCG GTTGAAAGTT 
CGCGCTGAGT CTTTTGACTC TTGGGCTAAC CGCGTGAAGG AAGCCCTAGA GCAGGATGAA 
GGCAACAAAA TAGATATTAA AGATCTGGAG GTATTAAAAG AAGAGGCAGC TGACAAGAAG 
TTCCCCAATA ATGAGCTGCT CCAGCGCCTC AACACTGTCT TCGTGGACAT AGAGAAGTGC 
GAGAGCTGCA GCACAGAACT CCTCAGCAAC AGCCAGAGCA GTAGAATGAC CCTGAAGGAG 
CTGAAGACTC TGGTGGACAC CATGCAGAAC CTGCCATGTG TTATAAAGCA GCAGGACGAA 
GTTCAGCTGA TATTGCAGAA GATAGAGGAG TTTGATTCCC GTGCTCAAGT TCTGGTCGAC 
AGCACCAAGA GTGAATGGAA GCAGGACTCT CCTCTTCCTG TGGCCACTGA GATCCAGGCT 
CTGCTAGAGG AAGGTGCATC ACTACCGGTT ATTGCCCCAG CTTGTGAGCT TTTGAGTGGG 
CTGTTGGAGC AGGGGCGCTG GCTGGGAGAG GTCAGGCGGA CGTTAGGGCC AGAGGGCAGT 
GAGGTGACTC TGACTGTGCT AAGGAACTTG ATGGAGTCGG GCTGTAATGT ACCACAGAGT 
GTGTCAGTGG AGACTGCAAT GGCTGAACTG CAAGAGCTCT TGACAATAGC TGAACGCTGG 
GAGGAGAAGG CGCAGATATG CTTGGAGGAC AGGCAGAAAC ACCATTTATC AACGCTGGAG 
GCTATTGTGA ATGAGGCTCA ACTAATACCA GTCCAGCTGC CTAATATCCT GTCCCTACAG 
GCCTGCTTGA GCCGAGCTCG GGCTTGGGTC ACAGATCTGG AGGAAATTCA GAATGGTGAG 
CACTATCCAT GTTTGGATGA CCTGGAGGGT CTGGTAGCCA TTGGGAGGGA CTTGCCAGTG 
AAGATGGAGG AGCTGAAGCA GCTGGAGCTG CAGGTGGCCA GTGCTCACTC CTGGAGAGAG 
AAGGCCAGCA AAACCTTTCT GAAGAAGAAC AGCCAACATA GTCTGCTCGA GGTGCTGTGC 
CCATGTGTTG AGAAGAGCAA GAAGATGGAG GACAACTCAA TGGTTACAGA AGCAGATTCG 
GATGTCAATG TATTAGGCCT CACAGCTCAG GACCTCAGAG ACCCAGGAGC TATTGTAATG 
GCATTTAAAG AAGGGGAGCG TCTGGAGAAA GAAGCCCTCC TCCGCCTCCA GCAGGTTAAT 
TTGGCGAAAC CCAGTATCGA AAATGGAGTA AGCACTGACC AGGACAGTAT CAAGACAGAA 
AAGAAACAGA GTAACTCCAT GGAGACAGAT GTGCCCCTTA ACTCAGACAG TCCCGTGCTG 
CAAAATGGAG GCTCTTCCCA TTCCCTCACC TCTAGCCAAA CTGTGTGTAT TTGTGGTGGC 
CCACCTCGCC CCTTGCTTCA CCGGTGCCAT CTGTGCAAAG ACTGGTTCCA TGGAGGTTGT 
GTGTCCTTCC CACCATTGTT GGCCACTTCA GACACGCATC TTACAAAACC CCTCTGCTGG 
TGGGACTGGA ACACGCGTTT TCTCTGTCCG CGGTGCCAGC GATCCCGACG GCCACGGCTG 
GAAACTATTC TAGCGCTGCT GGTGGCGCTG CAGAGGTTAC CTGTACGGCT GCCAGAGGGA 
GAAGCGTTAC AGTGTCTTAC AGAGAGAGCT ATTAACTGGC AAGGTCGAGC CAAACAGGCA 
TTAGACACTC CAGAAGTACA GGAGGCGCTA GACAGACTGC AACAAGTAGA GGATGAAATG 
GTCAGTATAA AAGAGGAAGA ACCAGAGGAG AAAAAGAAAT GGAATGGAGA TGCTGTAATT 
GTGCTTTCTG ACTCGGAGGA AGCAGAAGAT GGTGTTATCG ATCTGACGGA AGATGGGAAC 
TCGCCAAAAA AGAATGAAAG GAACGCTGTC AATGGTACAC AGTCTGGCTG TGAAAATGGA 
ATTGGCAGGA AGTCGAGTGC ACATGAAGTT ACAGGGGTGG AGTCTCTGTT GTCACTAGTG 
CCGAGTCTTA AAGGGCCAGT GGTGGAGCTG AACACATCTG TCAGAGCACA GCTGGAGGAG 
CTTCAGCTTG AAGGAGACCT GCTGGAGGTC ACGCTTGACC AGACACACGC CATCTACCGC 
ATCCTTCAGG CATCATCTCA GCCGCCCCAG GACACCTTGC GGACACTCAT ACAGATTGAA 
CTGCAGGAGC AGAGAAGCTC AGGTCGTGGC AACAAGTCGA AAGACTCCAA GAGGAAGAGA 
AAGAGCCAGA AAACCGAAGG CGAGCCGAAA TCCACAGAGG CTTCAGACTG CAAGAAGACG 
AAAGCCCTCA ATCTCAGCCC ACCTACAGAC ACACACACAG ATGTTGTGAT GTTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Kdm/Kmt gene families in the self-fertilizing mangrove rivulus fish, Kryptolebias marmoratus, suggest involvement of histone methylation machinery in development and reproduction.
Gene687173-187(2019 Mar)
Fellous A,Earley RL,Silvestre F

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

KDM7 is a dual demethylase for histone H3 Lys 9 and Lys 27 and functions in brain development.
Genes & development24(5)432-7(2010 Mar)
Tsukada Y,Ishitani T,Nakayama KI

The X-linked mental retardation gene SMCX/JARID1C defines a family of histone H3 lysine 4 demethylases.
Cell128(6)1077-88(2007 Mar)
Iwase S,Lan F,Bayliss P,de la Torre-Ubieta L,Huarte M,Qi HH,Whetstine JR,Bonni A,Roberts TM,Shi Y

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KDM5C cDNA ORF clone, Danio rerio(Zebrafish)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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