irs2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	irs2a
Entrez Gene ID	393285
Full Name	insulin receptor substrate 2a
Synonyms	zgc:56306
General protein information	Preferred Names insulin receptor substrate 2a Names insulin receptor substrate 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200315.1	NP_956609.1	insulin receptor substrate 2
XM_021466764.1	XP_021322439.1	insulin receptor substrate 2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	IRS2	XP_529580.4
Mus musculus (house mouse)	Irs2	NP_001074681.1
Danio rerio (zebrafish)	irs2	XP_700746.4
Rattus norvegicus (Norway rat)	Irs2	NP_001162104.1
Gallus gallus (chicken)	IRS2	XP_425588.4
Danio rerio (zebrafish)	zgc:56306	NP_956609.1
Canis lupus familiaris (dog)	IRS2	XP_542667.4
Xenopus tropicalis (tropical clawed frog)	LOC100498409	XP_004912043.1
Homo sapiens (human)	IRS2	NP_003740.2
Macaca mulatta (Rhesus monkey)	IRS2	XP_002800884.1
Bos taurus (cattle)	IRS2	XP_005228650.1

Gene Ontology
Bibliography

Related articles in PubMed

Impairment of bisphenol F on the glucose metabolism of zebrafish larvae.
Zhao F, Wang H, Wei P, Jiang G, Wang W, Zhang X, Ru S
Ecotoxicology and environmental safety165386-392(2018 Dec)

Different physiological roles of insulin receptors in mediating nutrient metabolism in zebrafish.
Yang BY, Zhai G, Gong YL, Su JZ, Peng XY, Shang GH, Han D, Jin JY, Liu HK, Du ZY, Yin Z, Xie SQ
American journal of physiology. Endocrinology and metabolism315(1)E38-E51(2018 07)

Comparative Study of Different Diets-Induced NAFLD Models of Zebrafish.
Chen B, Zheng YM, Zhang JP
Frontiers in endocrinology9366(2018)

Intracellular Insulin and Impaired Autophagy in a Zebrafish model and a Cell Model of Type 2 diabetes.
Meng XH, Chen B, Zhang JP
International journal of biological sciences13(8)985-995(2017)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following irs2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the irs2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33083	NM_200315.1 Latest version!	Danio rerio insulin receptor substrate 2a (irs2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa147213	XM_021466764.1 Latest version!	Danio rerio insulin receptor substrate 2a (irs2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

irs2a ( NM_200315.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956609.1 Danio rerio insulin receptor substrate 2a (irs2a), mRNA.

Price & Availability↑

CloneID	ODa33083
Clone ID Related Accession (Same CDS sequence)	NM_200315.1
Accession Version	NM_200315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549036800000
Organism	Danio rerio(zebrafish)
Product	insulin receptor substrate 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC050518.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050518.1, DN896592.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCAAGTC CGCCTCTTAA AGGGGGACCC TCGCTATCAA ACAGCAACAA CATTCATTCA 
AACAACATAC GAAAACATGG ATATCTCAAG AAGCAAAAAC ACGGACATCG GCGCTATTTT 
GTTTTGAAAG ACACGAGCGA CGGACTCCCA GCGCGCTTGG AATACCACGA GAATGAAAAG 
AAATGGAAAA ACAAGTCTGC TCCAAAAAGA GTGATATTCC TCGACTCGTG TTTGAGCATA 
AACAAGCGCG CTGACGCGAA ACACAGATAT TTGATAGCCC TCTATACCAA GGACGAATAT 
TTTGCTGTGG CTGCGGAGAA TGAGCAAGAG CAGGAGAGCT GGTACGGAGA CTTGACTGAT 
TTATTAAGCA AAGGAAAAGT GTGTGACAGC ACCGTTTCTA ATTCGGCATC CTCTCTGGTA 
GGCTTCGACG AGGGAAATTA CGGAATGATT ACACCGGTGA ATGCTGCTTA TAAAGAGGTA 
TGGCAAGTGA ACCTTAAAGC AAAGGGACTT GGGCAGAGCA GAAATATCAC TGGAGTTTTT 
AGGCTGTGTT TAACAAGTCG GACTATTAGT TTTGTGAAAC TTAACTCCGA GGTGGCATCA 
GTTACCCTAC AGTTGATGAA TATTCGTAGG TGTGGGCACT CTGATAGCTT TTTCTTTATT 
GAAGTTGGAA GATCAGCCTC TACTGGACCT GGAGAACTGT GGATGCAAGC AGACGATTCT 
GTGGTGGCAC AAAATATACA TGAGACAATT TTAGAAGCTA TGAAAGCAAT GAAAGAGCTG 
TCAGAGTTCA GACCCCGCAG CAAAAGCCAG TCATCAGGAT CAAACCCCAT TTCTGTACCC 
ACTCGTCGGA ACCTAAACAA TTTACCTCCA AGTCAAACAG GGCTAGTGAG GAGGTCAAGG 
ACGGACAGTA CAGCAGCCAC ATCTCCCGTG ACCAAATTTT CTTCTTGTCG AATACGGACT 
GCAAGTGAAG GGGAAGGTAC CATGATCAGG CCAGTCTCTA TATCAGTATC TGAGAATGGA 
AGCCCAATGA CTGTTGGCCG GAACCACATG AGTAGATCTA ATACAGTTTC AGTAGGCTGT 
CGGACATCAG AACCATCACT ACTTAATCAC AGCAGGTCCA TGTCTATGAC CATGTACCAC 
TCTCCCCCAT CTGTTGTAAG TCCACTAAGT TTGTCTTCAA TCAGTATGAA TGGTTCTATC 
TCAAACAAAG CACACAGACC TTCCAGCTGT AGTGGTTCTG TTTCTGGGTC ACCAAATGAT 
TTTGGCTTTT TAACGTGTGA GGATTACAGT TCCAGCCCAG GGGATGTGAG GTACAACCTA 
GCAAACAGGA GTGACACTCC TTCTTCTCTG TCCAGCACAC CACCCTTACG AGAAACCAGT 
GAACTCCATA GTTATATGGT GATGGACAGG CCATCCAACT TTTCACAGAA CAAGACTAGC 
AGAGACATGT TGGATGTGGA GACATACAGG AAGAGGACAT ATTCTCTAAC AACCCCACGG 
CAGCAGATGG CACACTCCCA GTTATCTTCA GCGTCACTTG ATGAATACAT GTTGATGAGG 
GCTGCGAACA GCCACTCTGG ACGGAGCACA AATTCTGCTT CTCCTAAACT TTCATATCCA 
GAAGATTATG GGGACATTGA GATAGGCTCT AACTGTAATG TGGGCGATGA TGGCTACATG 
CCAATGACAC CAGGCATGGC ACCTCAGAGT GCAAAAAATG ACCACTACAT GCCAATGAGT 
CCCATGAGTG TTTCAGCACC CAAGCAGATC ATTAACCCGA GATCTCATCC ACAGTCTCCA 
GGCAGTGGCT GCACAGCTAA CTCACCCAGC AGTGGATCTC TAGAGGACAG CGGGTACATG 
AAAATGTGGT CCGGATCCAA GTTTTCACTG GAGAGCTCAG ATGGAAAAGC TGCGAATGGA 
GAATATGTGA ATATGTCACC TGTTGACACA TCCATCTCAC CCACACCACC AGAATATTTT 
ATGGGTCCGT GTGAGCCAAC ACAACGGCCC TCCCCAACTC AAAGTATTCG CTTCAATAAG 
CAACAGGTGC CTAAAAGAGT AGATGATAAC CAGTATGTTT TCATGAGTCC TCAGAACCAA 
AATCAAGCCG AAGAGGCCAA CAGCGTTTCT TCTCTGCCCC CTCTCTATCG CCCTGATGTA 
ATGGCACAGA GATCAAAGGT CAACAGGCCC AATAGGTTAT CTCTAGACGC CCTAAGGATG 
CTTCCAAGGA TGACTGAACA TCCGCTACCA TGTGAGCCTA AAAGCCCTGG AGAGTACATA 
AACATAGACT TTGCTGCTGC TACACAGTAC TCCTCTCAAT CTATGGTCAC TGTGGAAAAT 
CAGGCCTCAC TGTCTAGCCT CAGACAGGGA TCTCCGCTCT CAGACTACAT GAACCTCAAC 
CAGAGTTCAC ACTCTTCTAA ACTGTGTGAA ATGAGTAGTC CCCTCGATGG ACTGCCAGAG 
CTGGCTGAAT GCCCGCCTGA TGATGGGACT TGTTTTCTCA ACGAACAGAG AAACTTACCA 
TGCCCCTCTG TATCAGGAAA AGGTAATTAC ACTGAGATGA GCTTCAACAG TGGTTCCCTT 
GAAAATCAAA AGAGTGCTTC AGTCAGCCCC ACTAACAGGG TTCAAAGACT TAACTTGGGC 
AACCAGGGGG TTTCAAAAGG AATTGGCGCT TTCTTGTTAG CTGCCTCCTC TGTAGATCCA 
AACGGAGGAG CAAAGGTGAT TCGAGCAGAC CCCCAGGGCC GGAGACGGCA TAGCTCTGAG 
ACCTTTTCAT CCACCACCAC AGTTGCACCG GTCTTTCCCT CTTTTGCACA CAATGTTAAG 
CGACACAGCT CTGCCTCCGT AGAAAACATC TCAGTACGAA GCAGTGAAGG TTCTGATGAG 
GAGTATGAGT CTCCTATGTG CCAAGAAATG TCAGCTGGTT ATCAAAATGG GCTCAATTAC 
ATCGCCTTAA ATCTCATGGA GAAACAAGAG ACTGGCAATT GTGAAAATCT TGTGGGCTTT 
AAGACTGGCG GTTGTTGTAA AGCAGGAATA AATGGATTAC ACACAACTCC CTATGTGTGT 
CTTGGATTCA AAGAAACTGC AACCACTGTA CAAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956609.1
CDS	152..3250
Translation	MASPPLKGGPSLSNSNNIHSNNIRKHGYLKKQKHGHRRYFVLKDTSDGLPARLEYHENEKKWKNKSAPKRVIFLDSCLSINKRADAKHRYLIALYTKDEYFAVAAENEQEQESWYGDLTDLLSKGKVCDSTVSNSASSLVGFDEGNYGMITPVNAAYKEVWQVNLKAKGLGQSRNITGVFRLCLTSRTISFVKLNSEVASVTLQLMNIRRCGHSDSFFFIEVGRSASTGPGELWMQADDSVVAQNIHETILEAMKAMKELSEFRPRSKSQSSGSNPISVPTRRNLNNLPPSQTGLVRRSRTDSTAATSPVTKFSSCRIRTASEGEGTMIRPVSISVSENGSPMTVGRNHMSRSNTVSVGCRTSEPSLLNHSRSMSMTMYHSPPSVVSPLSLSSISMNGSISNKAHRPSSCSGSVSGSPNDFGFLTCEDYSSSPGDVRYNLANRSDTPSSLSSTPPLRETSELHSYMVMDRPSNFSQNKTSRDMLDVETYRKRTYSLTTPRQQMAHSQLSSASLDEYMLMRAANSHSGRSTNSASPKLSYPEDYGDIEIGSNCNVGDDGYMPMTPGMAPQSAKNDHYMPMSPMSVSAPKQIINPRSHPQSPGSGCTANSPSSGSLEDSGYMKMWSGSKFSLESSDGKAANGEYVNMSPVDTSISPTPPEYFMGPCEPTQRPSPTQSIRFNKQQVPKRVDDNQYVFMSPQNQNQAEEANSVSSLPPLYRPDVMAQRSKVNRPNRLSLDALRMLPRMTEHPLPCEPKSPGEYINIDFAAATQYSSQSMVTVENQASLSSLRQGSPLSDYMNLNQSSHSSKLCEMSSPLDGLPELAECPPDDGTCFLNEQRNLPCPSVSGKGNYTEMSFNSGSLENQKSASVSPTNRVQRLNLGNQGVSKGIGAFLLAASSVDPNGGAKVIRADPQGRRRHSSETFSSTTTVAPVFPSFAHNVKRHSSASVENISVRSSEGSDEEYESPMCQEMSAGYQNGLNYIALNLMEKQETGNCENLVGFKTGGCCKAGINGLHTTPYVCLGFKETATTVQD

Target ORF information:

RefSeq Version	NM_200315.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio insulin receptor substrate 2a (irs2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200315.1

ORF Insert Sequence:

ATGGCAAGTC CGCCTCTTAA AGGGGGACCC TCGCTATCAA ACAGCAACAA CATTCATTCA 
AACAACATAC GAAAACATGG ATATCTCAAG AAGCAAAAAC ACGGACATCG GCGCTATTTT 
GTTTTGAAAG ACACGAGCGA CGGACTCCCA GCGCGCTTGG AATACCACGA GAATGAAAAG 
AAATGGAAAA ACAAGTCTGC TCCAAAAAGA GTGATATTCC TCGACTCGTG TTTGAGCATA 
AACAAGCGCG CTGACGCGAA ACACAGATAT TTGATAGCCC TCTATACCAA GGACGAATAT 
TTTGCTGTGG CTGCGGAGAA TGAGCAAGAG CAGGAGAGCT GGTACGGAGA CTTGACTGAT 
TTATTAAGCA AAGGAAAAGT GTGTGACAGC ACCGTTTCTA ATTCGGCATC CTCTCTGGTA 
GGCTTCGACG AGGGAAATTA CGGAATGATT ACACCGGTGA ATGCTGCTTA TAAAGAGGTA 
TGGCAAGTGA ACCTTAAAGC AAAGGGACTT GGGCAGAGCA GAAATATCAC TGGAGTTTTT 
AGGCTGTGTT TAACAAGTCG GACTATTAGT TTTGTGAAAC TTAACTCCGA GGTGGCATCA 
GTTACCCTAC AGTTGATGAA TATTCGTAGG TGTGGGCACT CTGATAGCTT TTTCTTTATT 
GAAGTTGGAA GATCAGCCTC TACTGGACCT GGAGAACTGT GGATGCAAGC AGACGATTCT 
GTGGTGGCAC AAAATATACA TGAGACAATT TTAGAAGCTA TGAAAGCAAT GAAAGAGCTG 
TCAGAGTTCA GACCCCGCAG CAAAAGCCAG TCATCAGGAT CAAACCCCAT TTCTGTACCC 
ACTCGTCGGA ACCTAAACAA TTTACCTCCA AGTCAAACAG GGCTAGTGAG GAGGTCAAGG 
ACGGACAGTA CAGCAGCCAC ATCTCCCGTG ACCAAATTTT CTTCTTGTCG AATACGGACT 
GCAAGTGAAG GGGAAGGTAC CATGATCAGG CCAGTCTCTA TATCAGTATC TGAGAATGGA 
AGCCCAATGA CTGTTGGCCG GAACCACATG AGTAGATCTA ATACAGTTTC AGTAGGCTGT 
CGGACATCAG AACCATCACT ACTTAATCAC AGCAGGTCCA TGTCTATGAC CATGTACCAC 
TCTCCCCCAT CTGTTGTAAG TCCACTAAGT TTGTCTTCAA TCAGTATGAA TGGTTCTATC 
TCAAACAAAG CACACAGACC TTCCAGCTGT AGTGGTTCTG TTTCTGGGTC ACCAAATGAT 
TTTGGCTTTT TAACGTGTGA GGATTACAGT TCCAGCCCAG GGGATGTGAG GTACAACCTA 
GCAAACAGGA GTGACACTCC TTCTTCTCTG TCCAGCACAC CACCCTTACG AGAAACCAGT 
GAACTCCATA GTTATATGGT GATGGACAGG CCATCCAACT TTTCACAGAA CAAGACTAGC 
AGAGACATGT TGGATGTGGA GACATACAGG AAGAGGACAT ATTCTCTAAC AACCCCACGG 
CAGCAGATGG CACACTCCCA GTTATCTTCA GCGTCACTTG ATGAATACAT GTTGATGAGG 
GCTGCGAACA GCCACTCTGG ACGGAGCACA AATTCTGCTT CTCCTAAACT TTCATATCCA 
GAAGATTATG GGGACATTGA GATAGGCTCT AACTGTAATG TGGGCGATGA TGGCTACATG 
CCAATGACAC CAGGCATGGC ACCTCAGAGT GCAAAAAATG ACCACTACAT GCCAATGAGT 
CCCATGAGTG TTTCAGCACC CAAGCAGATC ATTAACCCGA GATCTCATCC ACAGTCTCCA 
GGCAGTGGCT GCACAGCTAA CTCACCCAGC AGTGGATCTC TAGAGGACAG CGGGTACATG 
AAAATGTGGT CCGGATCCAA GTTTTCACTG GAGAGCTCAG ATGGAAAAGC TGCGAATGGA 
GAATATGTGA ATATGTCACC TGTTGACACA TCCATCTCAC CCACACCACC AGAATATTTT 
ATGGGTCCGT GTGAGCCAAC ACAACGGCCC TCCCCAACTC AAAGTATTCG CTTCAATAAG 
CAACAGGTGC CTAAAAGAGT AGATGATAAC CAGTATGTTT TCATGAGTCC TCAGAACCAA 
AATCAAGCCG AAGAGGCCAA CAGCGTTTCT TCTCTGCCCC CTCTCTATCG CCCTGATGTA 
ATGGCACAGA GATCAAAGGT CAACAGGCCC AATAGGTTAT CTCTAGACGC CCTAAGGATG 
CTTCCAAGGA TGACTGAACA TCCGCTACCA TGTGAGCCTA AAAGCCCTGG AGAGTACATA 
AACATAGACT TTGCTGCTGC TACACAGTAC TCCTCTCAAT CTATGGTCAC TGTGGAAAAT 
CAGGCCTCAC TGTCTAGCCT CAGACAGGGA TCTCCGCTCT CAGACTACAT GAACCTCAAC 
CAGAGTTCAC ACTCTTCTAA ACTGTGTGAA ATGAGTAGTC CCCTCGATGG ACTGCCAGAG 
CTGGCTGAAT GCCCGCCTGA TGATGGGACT TGTTTTCTCA ACGAACAGAG AAACTTACCA 
TGCCCCTCTG TATCAGGAAA AGGTAATTAC ACTGAGATGA GCTTCAACAG TGGTTCCCTT 
GAAAATCAAA AGAGTGCTTC AGTCAGCCCC ACTAACAGGG TTCAAAGACT TAACTTGGGC 
AACCAGGGGG TTTCAAAAGG AATTGGCGCT TTCTTGTTAG CTGCCTCCTC TGTAGATCCA 
AACGGAGGAG CAAAGGTGAT TCGAGCAGAC CCCCAGGGCC GGAGACGGCA TAGCTCTGAG 
ACCTTTTCAT CCACCACCAC AGTTGCACCG GTCTTTCCCT CTTTTGCACA CAATGTTAAG 
CGACACAGCT CTGCCTCCGT AGAAAACATC TCAGTACGAA GCAGTGAAGG TTCTGATGAG 
GAGTATGAGT CTCCTATGTG CCAAGAAATG TCAGCTGGTT ATCAAAATGG GCTCAATTAC 
ATCGCCTTAA ATCTCATGGA GAAACAAGAG ACTGGCAATT GTGAAAATCT TGTGGGCTTT 
AAGACTGGCG GTTGTTGTAA AGCAGGAATA AATGGATTAC ACACAACTCC CTATGTGTGT 
CTTGGATTCA AAGAAACTGC AACCACTGTA CAAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

irs2a ( XM_021466764.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322439.1 Danio rerio insulin receptor substrate 2a (irs2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa147213
Clone ID Related Accession (Same CDS sequence)	XM_021466764.1
Accession Version	XM_021466764.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	insulin receptor substrate 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGTC CGCCTCTTAA AGGGGGACCC TCGCTATCAA ACAGCAACAA CATTCATTCA 
AACAACATAC GAAAACATGG ATATCTCAAG AAGCAAAAAC ACGGACATCG GCGCTATTTT 
GTTTTGAAAG ACACGAGCGA CGGACTCCCA GCGCGCTTGG AATACCACGA GAATGAAAAG 
AAATGGAAAA ACAAGTCTGC TCCAAAAAGA GTGATATTCC TCGACTCGTG TTTGAGCATA 
AACAAGCGCG CTGACGCGAA ACACAGATAT TTGATAGCCC TCTATACCAA GGACGAATAT 
TTTGCTGTGG CTGCGGAGAA TGAGCAAGAG CAGGAGAGCT GGTACGGAGA CTTGGCTGAT 
TTATTAAGCA AAGGAAAAGT GTGTGACAGC ACCGTTTCTA ATTCGGCATC CTCTCTGGTA 
GGCTTCGACG AGGGAAATTA CGGAATGATT ACACCGGTGA ATGCTGCTTA TAAAGAGGTA 
TGGCAAGTGA ACCTTAAAGC AAAGGGACTT GGGCAGAGCA GAAATATCAC TGGAGTTTTT 
AGGCTGTGTT TAACAAGTCG GACTATTAGT TTTGTGAAAC TTAACTCCGA GGTGGCATCA 
GTTACCCTAC AGTTGATGAA TATTCGTAGG TGTGGGCACT CTGATAGCTT TTTCTTTATT 
GAAGTTGGAA GATCAGCCTC TACTGGACCT GGAGAACTGT GGATGCAAGC AGACGATTCT 
GTGGTGGCAC AAAATATACA TGAGACAATT TTAGAAGCTA TGAAAGCAAT GAAAGAGCTG 
TCAGAGTTCA GACCCCGCAG CAAAAGTCAG TCATCAGGAT CAAACCCCAT TTCTGTACCC 
ACTCGTCGGA ACCTAAACAA TTTACCTCCA AGTCAAACAG GGCTAGTGAG GAGGTCAAGG 
ACGGACAGTA CAGCAGCCAC ATCTCCCGTG ACCAAATTTT CTTCTTGTCG AATACGGACT 
GCAAGTGAAG GGGAAGGTAC CATGATCAGG CCAGTCTCTG TATCAGTATC TGAGAATGGA 
AGCCCAATGA CTGTTGGCCG GCACCACATG AGTAGATCTA ATACTGTTTC AGTAGGCTGT 
CGGACATCAG AACCATCACT ACTTAATCAC AGCAGGTCCA TGTCTATGAC CATGTACCAC 
TCTCCCCCAT CTGTTGTAAG TCCACTAAGT TTGTCTTCAA TCAGTATGAA TGGTTCTATC 
TCAAACAAAG CACACAGACC TTCCAGCTGT AGTGGTTCTG TTTCTGGGTC ACCAAATGAT 
TTTGGCTTTT TAACGTGTGA GGATTACAGT TCCAGCCCAG GGGATGTGAG GTACAACCTA 
GCAAACAGGA GTGACACTCC TTCTTCTCTG TCCAGCACAC CACCCTTACG AGAAACCAGT 
GAACTCCATA GTTATATGGT GATGGACAGG CCATCCAACT TTTCACAGAA CAAGACTAGC 
AGAGACATGT TGGATGTGGA GACATACAGG AAGAGGACAT ATTCTCTAAC AACCCCACGG 
CAACAGATGG CACACTCCCA GTTATCTTCA GCGTCACTTG ATGAATACAT GTTGATGAGG 
GCTGCGAACA GCCACTCTGG ACGGAGCACA AATTCTGCTT CTCCTAAACT TTCATATCCA 
GAAGATTATG GGGACATTGA GATAGGCTCT AACTGTAATG TGGGTGATGA TGGCTACATG 
CCAATGACAC CAGGCATGGC ACCTCAGAGT GCAAAAAATG ACCACTACAT GCCAATGAGT 
CCCATGAGTG TTTCAGCACC CAAGCAGATC ATTAACCCGA GATCTCATCC ACAGTCTCCA 
GGCAGTGGCT GCACAGCTAA CTCCCCCAGC AGTGGATCTC TAGAGGACAG CGGGTACATG 
AAAATGTGGT CCGGATCCAA GTTTTCACTG GAGAGCTCAG ATGGAAAAGC AGCGAATGGA 
GAATATGTGA ATATGTCACC TGTTGACACA TCCATCTCAC CCACACCACC AGAATATTTT 
ATGGGTCCTT GTGAGCCAAC ACAACGGCCC TCCCCAACTC AAAGTATTCG CTTCAATAAG 
CAACAGGTGC CTAAAAGAGT AGATGATAAC CAGTATGTTT TCATGAGTCC TCAGAACCAA 
AATCAAACCG AAGAGGCCAA CAGCGTTTCT TCTCTGCCCC CTCTCTATCG CCCTGATGTA 
ATGGCACAGA GATCAAAGGT CAACAGGCCC AATAGGTTAT CTCTAGACGC TCTAAGGATG 
CTTCCAAGGA TGACTGAACA TCCGCTACCA TGTGAGCCTA AAAGCCCTGG AGAGTACATA 
AACATAGACT TTGCTGCTGC TACTCAGTAC TCCTCTCAAT CTATGGTCAC TGTGGAAAAT 
CAGGCCTCAC TGTCCAGCCT CAGACAGGGA TCTCCGCTCT CAGACTACAT GAACCTCAAC 
CAGAGTTCAC ACTCTTCTAA ACTGTGTGAA ATGAGTAGTC CCCTCGATGG ACTGCCAGAG 
CTGGCTGAAT GCCCGCCTGA TGATGGGACT TGTTTTCTCA ACGAACAGAG AAACTTACCA 
TGCCCCTCTG TATCAGGAAA AGGTAATTAC ACTGAGATGA GCTTCAACAG TGGTTCCCTT 
GAAAATCAAA AGAGTGCTTC AGTCAGCCCC ACTAGCAGGG TTCAAAGGCT TAACTTGGGC 
AACCAGGGGG TTTCAAAAGG AATTGGCGCT TTCTTGTTAG CTGCCTCCTC TGTAGATCCA 
AACGGAGGAG CAAAGGTGAT TCGAGCAGAC CCCCAGGGCC GGAGACGGCA TAGCTCTGAG 
ACCTTTTCAT CCACCACCAC AGTTGCACCG GTCTTTCCCT CTTTTGCACA CAATGTTAAG 
CGACACAGCT CTGCCTCCGT AGAAAACATC TCAGTACGAA GCAGTGAAGG TTCTGATGAG 
GAGTATGAGT CTCCTATGTG CCAAGAAATG TCAGCTGGTT ATCAAAATGG GCTCAATTAC 
ATCGCCTTAA ATCTCATGGA GAAACAAGAG ACTGGCAATT GTGAAAATCT TGTGGGCTTT 
AAGACTGGCG GTTGTTGTAA AGCAGGAATA AATGGATTAC ACACAACTCC CTATGTGTGT 
CTTGGATTCA AAGAAACTGC AACCACTGTA CAAGTACTCC TTAAGACGAT TGTGTCCATT 
TGCATGTGTG GCAGATGGCT GAATAGATGT GGGGCTGTGT GTGTGTGTGC TCGCGTGCAC 
CTGCCGCTCT GCTCACCGCA GATTGAGCAG GAAGCAGAGG TTACTCCTAA TGCTAAGGGA 
AAATGGAAGC TCAACCATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322439.1
CDS	302..3562
Translation	MASPPLKGGPSLSNSNNIHSNNIRKHGYLKKQKHGHRRYFVLKDTSDGLPARLEYHENEKKWKNKSAPKRVIFLDSCLSINKRADAKHRYLIALYTKDEYFAVAAENEQEQESWYGDLADLLSKGKVCDSTVSNSASSLVGFDEGNYGMITPVNAAYKEVWQVNLKAKGLGQSRNITGVFRLCLTSRTISFVKLNSEVASVTLQLMNIRRCGHSDSFFFIEVGRSASTGPGELWMQADDSVVAQNIHETILEAMKAMKELSEFRPRSKSQSSGSNPISVPTRRNLNNLPPSQTGLVRRSRTDSTAATSPVTKFSSCRIRTASEGEGTMIRPVSVSVSENGSPMTVGRHHMSRSNTVSVGCRTSEPSLLNHSRSMSMTMYHSPPSVVSPLSLSSISMNGSISNKAHRPSSCSGSVSGSPNDFGFLTCEDYSSSPGDVRYNLANRSDTPSSLSSTPPLRETSELHSYMVMDRPSNFSQNKTSRDMLDVETYRKRTYSLTTPRQQMAHSQLSSASLDEYMLMRAANSHSGRSTNSASPKLSYPEDYGDIEIGSNCNVGDDGYMPMTPGMAPQSAKNDHYMPMSPMSVSAPKQIINPRSHPQSPGSGCTANSPSSGSLEDSGYMKMWSGSKFSLESSDGKAANGEYVNMSPVDTSISPTPPEYFMGPCEPTQRPSPTQSIRFNKQQVPKRVDDNQYVFMSPQNQNQTEEANSVSSLPPLYRPDVMAQRSKVNRPNRLSLDALRMLPRMTEHPLPCEPKSPGEYINIDFAAATQYSSQSMVTVENQASLSSLRQGSPLSDYMNLNQSSHSSKLCEMSSPLDGLPELAECPPDDGTCFLNEQRNLPCPSVSGKGNYTEMSFNSGSLENQKSASVSPTSRVQRLNLGNQGVSKGIGAFLLAASSVDPNGGAKVIRADPQGRRRHSSETFSSTTTVAPVFPSFAHNVKRHSSASVENISVRSSEGSDEEYESPMCQEMSAGYQNGLNYIALNLMEKQETGNCENLVGFKTGGCCKAGINGLHTTPYVCLGFKETATTVQVLLKTIVSICMCGRWLNRCGAVCVCARVHLPLCSPQIEQEAEVTPNAKGKWKLNH

Target ORF information:

RefSeq Version	XM_021466764.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio insulin receptor substrate 2a (irs2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466764.1

ORF Insert Sequence:

ATGGCAAGTC CGCCTCTTAA AGGGGGACCC TCGCTATCAA ACAGCAACAA CATTCATTCA 
AACAACATAC GAAAACATGG ATATCTCAAG AAGCAAAAAC ACGGACATCG GCGCTATTTT 
GTTTTGAAAG ACACGAGCGA CGGACTCCCA GCGCGCTTGG AATACCACGA GAATGAAAAG 
AAATGGAAAA ACAAGTCTGC TCCAAAAAGA GTGATATTCC TCGACTCGTG TTTGAGCATA 
AACAAGCGCG CTGACGCGAA ACACAGATAT TTGATAGCCC TCTATACCAA GGACGAATAT 
TTTGCTGTGG CTGCGGAGAA TGAGCAAGAG CAGGAGAGCT GGTACGGAGA CTTGGCTGAT 
TTATTAAGCA AAGGAAAAGT GTGTGACAGC ACCGTTTCTA ATTCGGCATC CTCTCTGGTA 
GGCTTCGACG AGGGAAATTA CGGAATGATT ACACCGGTGA ATGCTGCTTA TAAAGAGGTA 
TGGCAAGTGA ACCTTAAAGC AAAGGGACTT GGGCAGAGCA GAAATATCAC TGGAGTTTTT 
AGGCTGTGTT TAACAAGTCG GACTATTAGT TTTGTGAAAC TTAACTCCGA GGTGGCATCA 
GTTACCCTAC AGTTGATGAA TATTCGTAGG TGTGGGCACT CTGATAGCTT TTTCTTTATT 
GAAGTTGGAA GATCAGCCTC TACTGGACCT GGAGAACTGT GGATGCAAGC AGACGATTCT 
GTGGTGGCAC AAAATATACA TGAGACAATT TTAGAAGCTA TGAAAGCAAT GAAAGAGCTG 
TCAGAGTTCA GACCCCGCAG CAAAAGTCAG TCATCAGGAT CAAACCCCAT TTCTGTACCC 
ACTCGTCGGA ACCTAAACAA TTTACCTCCA AGTCAAACAG GGCTAGTGAG GAGGTCAAGG 
ACGGACAGTA CAGCAGCCAC ATCTCCCGTG ACCAAATTTT CTTCTTGTCG AATACGGACT 
GCAAGTGAAG GGGAAGGTAC CATGATCAGG CCAGTCTCTG TATCAGTATC TGAGAATGGA 
AGCCCAATGA CTGTTGGCCG GCACCACATG AGTAGATCTA ATACTGTTTC AGTAGGCTGT 
CGGACATCAG AACCATCACT ACTTAATCAC AGCAGGTCCA TGTCTATGAC CATGTACCAC 
TCTCCCCCAT CTGTTGTAAG TCCACTAAGT TTGTCTTCAA TCAGTATGAA TGGTTCTATC 
TCAAACAAAG CACACAGACC TTCCAGCTGT AGTGGTTCTG TTTCTGGGTC ACCAAATGAT 
TTTGGCTTTT TAACGTGTGA GGATTACAGT TCCAGCCCAG GGGATGTGAG GTACAACCTA 
GCAAACAGGA GTGACACTCC TTCTTCTCTG TCCAGCACAC CACCCTTACG AGAAACCAGT 
GAACTCCATA GTTATATGGT GATGGACAGG CCATCCAACT TTTCACAGAA CAAGACTAGC 
AGAGACATGT TGGATGTGGA GACATACAGG AAGAGGACAT ATTCTCTAAC AACCCCACGG 
CAACAGATGG CACACTCCCA GTTATCTTCA GCGTCACTTG ATGAATACAT GTTGATGAGG 
GCTGCGAACA GCCACTCTGG ACGGAGCACA AATTCTGCTT CTCCTAAACT TTCATATCCA 
GAAGATTATG GGGACATTGA GATAGGCTCT AACTGTAATG TGGGTGATGA TGGCTACATG 
CCAATGACAC CAGGCATGGC ACCTCAGAGT GCAAAAAATG ACCACTACAT GCCAATGAGT 
CCCATGAGTG TTTCAGCACC CAAGCAGATC ATTAACCCGA GATCTCATCC ACAGTCTCCA 
GGCAGTGGCT GCACAGCTAA CTCCCCCAGC AGTGGATCTC TAGAGGACAG CGGGTACATG 
AAAATGTGGT CCGGATCCAA GTTTTCACTG GAGAGCTCAG ATGGAAAAGC AGCGAATGGA 
GAATATGTGA ATATGTCACC TGTTGACACA TCCATCTCAC CCACACCACC AGAATATTTT 
ATGGGTCCTT GTGAGCCAAC ACAACGGCCC TCCCCAACTC AAAGTATTCG CTTCAATAAG 
CAACAGGTGC CTAAAAGAGT AGATGATAAC CAGTATGTTT TCATGAGTCC TCAGAACCAA 
AATCAAACCG AAGAGGCCAA CAGCGTTTCT TCTCTGCCCC CTCTCTATCG CCCTGATGTA 
ATGGCACAGA GATCAAAGGT CAACAGGCCC AATAGGTTAT CTCTAGACGC TCTAAGGATG 
CTTCCAAGGA TGACTGAACA TCCGCTACCA TGTGAGCCTA AAAGCCCTGG AGAGTACATA 
AACATAGACT TTGCTGCTGC TACTCAGTAC TCCTCTCAAT CTATGGTCAC TGTGGAAAAT 
CAGGCCTCAC TGTCCAGCCT CAGACAGGGA TCTCCGCTCT CAGACTACAT GAACCTCAAC 
CAGAGTTCAC ACTCTTCTAA ACTGTGTGAA ATGAGTAGTC CCCTCGATGG ACTGCCAGAG 
CTGGCTGAAT GCCCGCCTGA TGATGGGACT TGTTTTCTCA ACGAACAGAG AAACTTACCA 
TGCCCCTCTG TATCAGGAAA AGGTAATTAC ACTGAGATGA GCTTCAACAG TGGTTCCCTT 
GAAAATCAAA AGAGTGCTTC AGTCAGCCCC ACTAGCAGGG TTCAAAGGCT TAACTTGGGC 
AACCAGGGGG TTTCAAAAGG AATTGGCGCT TTCTTGTTAG CTGCCTCCTC TGTAGATCCA 
AACGGAGGAG CAAAGGTGAT TCGAGCAGAC CCCCAGGGCC GGAGACGGCA TAGCTCTGAG 
ACCTTTTCAT CCACCACCAC AGTTGCACCG GTCTTTCCCT CTTTTGCACA CAATGTTAAG 
CGACACAGCT CTGCCTCCGT AGAAAACATC TCAGTACGAA GCAGTGAAGG TTCTGATGAG 
GAGTATGAGT CTCCTATGTG CCAAGAAATG TCAGCTGGTT ATCAAAATGG GCTCAATTAC 
ATCGCCTTAA ATCTCATGGA GAAACAAGAG ACTGGCAATT GTGAAAATCT TGTGGGCTTT 
AAGACTGGCG GTTGTTGTAA AGCAGGAATA AATGGATTAC ACACAACTCC CTATGTGTGT 
CTTGGATTCA AAGAAACTGC AACCACTGTA CAAGTACTCC TTAAGACGAT TGTGTCCATT 
TGCATGTGTG GCAGATGGCT GAATAGATGT GGGGCTGTGT GTGTGTGTGC TCGCGTGCAC 
CTGCCGCTCT GCTCACCGCA GATTGAGCAG GAAGCAGAGG TTACTCCTAA TGCTAAGGGA 
AAATGGAAGC TCAACCATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Impairment of bisphenol F on the glucose metabolism of zebrafish larvae.
Ecotoxicology and environmental safety165386-392(2018 Dec)
Zhao F,Wang H,Wei P,Jiang G,Wang W,Zhang X,Ru S

Different physiological roles of insulin receptors in mediating nutrient metabolism in zebrafish.
American journal of physiology. Endocrinology and metabolism315(1)E38-E51(2018 07)
Yang BY,Zhai G,Gong YL,Su JZ,Peng XY,Shang GH,Han D,Jin JY,Liu HK,Du ZY,Yin Z,Xie SQ

Comparative Study of Different Diets-Induced NAFLD Models of Zebrafish.
Frontiers in endocrinology9366(2018)
Chen B,Zheng YM,Zhang JP

Intracellular Insulin and Impaired Autophagy in a Zebrafish model and a Cell Model of Type 2 diabetes.
International journal of biological sciences13(8)985-995(2017)
Meng XH,Chen B,Zhang JP

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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irs2a cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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