rxfp1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rxfp1
Entrez Gene ID	100498669
Full Name	relaxin/insulin-like family peptide receptor 1
General protein information	Preferred Names relaxin/insulin-like family peptide receptor 1 Names relaxin receptor 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009291265.2	XP_009289540.1	relaxin receptor 1 isoform X1
XM_009291265.3	XP_009289540.1	relaxin receptor 1 isoform X1
NM_001190934.1	NP_001177863.1	relaxin receptor 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RXFP1	XP_001144181.2
Bos taurus (cattle)	RXFP1	XP_002694461.1
Mus musculus (house mouse)	Rxfp1	NP_997617.1
Gallus gallus (chicken)	RXFP1	XP_420385.2
Danio rerio (zebrafish)	rxfp1	NP_001177863.1
Homo sapiens (human)	RXFP1	NP_001240656.1
Canis lupus familiaris (dog)	RXFP1	XP_005629433.1
Macaca mulatta (Rhesus monkey)	RXFP1	XP_001096574.1
Anopheles gambiae (African malaria mosquito)	GPRGPH	XP_321252.4
Drosophila melanogaster (fruit fly)	CG34411	NP_001096966.2
Rattus norvegicus (Norway rat)	Rxfp1	NP_958820.1
Xenopus tropicalis (tropical clawed frog)	rxfp1	XP_002934794.2

Gene Ontology
Bibliography

Related articles in PubMed

Japanese medaka as a model for studying the relaxin family genes involved in neuroendocrine regulation: Insights from the expression of fish-specific rln3 and insl5 and rxfp3/4-type receptor paralogues.
Alnafea H, Vahkal B, Zelmer CK, Yegorov S, Bogerd J, Good SV
Molecular and cellular endocrinology4872-11(2019 05)

Functional characterization of zebrafish orthologs of the human Beta 3-Glucosyltransferase B3GLCT gene mutated in Peters Plus Syndrome.
Weh E, Takeuchi H, Muheisen S, Haltiwanger RS, Semina EV
PloS one12(9)e0184903(2017)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

New insights into ligand-receptor pairing and coevolution of relaxin family peptides and their receptors in teleosts.
Good S, Yegorov S, Martijn J, Franck J, Bogerd J
International journal of evolutionary biology2012310278(2012)

Characterization and developmental expression pattern of the relaxin receptor rxfp1 gene in zebrafish.
Donizetti A, Fiengo M, del Gaudio R, Di Giaimo R, Minucci S, Aniello F
Development, growth & differentiation52(9)799-806(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rxfp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rxfp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa34385	NM_001190934.1 Latest version!	Danio rerio relaxin family peptide receptor 1 (rxfp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa50039	XM_009291265.3 Latest version!	Danio rerio relaxin/insulin-like family peptide receptor 1 (rxfp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
ODa50039	XM_009291265.2	Danio rerio relaxin/insulin-like family peptide receptor 1 (rxfp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rxfp1 ( NM_001190934.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001177863.1 Danio rerio relaxin family peptide receptor 1 (rxfp1), mRNA.

Price & Availability↑

CloneID	ODa34385
Clone ID Related Accession (Same CDS sequence)	NM_001190934.1
Accession Version	NM_001190934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568995200000
Organism	Danio rerio(zebrafish)
Product	relaxin receptor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from HM135955.1. ##Evidence-Data-START## Transcript exon combination :: HM135955.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

ATGCCTAATT TCTTTCTCCC GTCTGTGTTT TTCTCTTCTG AAGTGGTGCT GACCATGTTT 
AATGTATCAG AGGATCAGCC CTACTGCCCG CTGGGATATT TCCCCTGTGG AAACCTCAGC 
ACCTGCCTTC CTCAAGTGCT GCACTGCAAC GGCGTGGATG ACTGTGGAAA TCAGGCAGAC 
GAGGAAAACT GTGGAGACAA TAATGGCTGG CCACATCTCT TTGATAACTA TTTTGGCATC 
CCCAGTAATA ACCTTGGAAA CAAGTCAGAC GCCTGCTTGC TCGGGACCGT CCCGGCTGAG 
TGCCAGTGCA GAGATCTGGA GCTGGACTGT GATGGTGCAC ACTTTAAAGA CGTCCCAATG 
GTATCCATTA ATGTCACCAT GATGTCCCTG CAGAGGAACG GGCTGAGGAA ACTAAACGCT 
GACATGTTCT TGAAGTACCA GAGCCTTCAA AAGCTGTACC TCCAGCACAA CCGCATTAAA 
TCAGTTCACC CGCAGGCATT CAGAGGCCTT TACAATCTGA CCAGACTGTA TCTCAGCTAT 
AATCGAATCA CAACTCTTCT CCCTGATGTT TTCCAGGATT TACACAAACT AGAGTGGCTG 
ATTCTGGAGA ACAACAGCCT TCATCACATC TCCTCTTTGA CCTTCTCGGG TCTTCGCTCT 
CTTGTGCTGC TAGTGCTGTT GAACAATGCT CTCACAAAAC TGGATGATAT CTGCCTGGAG 
ATGCCACGGT TAAATTGGCT AGACATTGAG GGAAATAAGA TGGAGACGGT AGGAAACGTG 
ACCTTCAGGT CGTGTAACAT GCTAACAGTG CTGGTCCTGC AGCGGAACCG CATCAGTCGC 
ATCCACGCGC AAGCTTTCTC TCTGCTGCGG AAACTGGGAG AGCTGGATTT GTCCAGTAAC 
AGAATCGAAG CGATCCCGCC TGATCTTTTC GTCAATCTCG GAGACCTGCT GCAGCTGAAT 
ATTTCATATA ACCCGATCAT GAACCTGCGG GTGGACCACT TTGACAAGCT GCATAAGTTA 
AAGTCACTGA GCATTGAGGG AATCGAAATT GGGAACATTC ACAGACGGAT GTTTGAACCT 
CTCAAGAACC TGACTCACAT CTACTTTAAA AAGTTCCAGT ACTGTGGTTA TGCTCCTTAC 
GTCCGCAGCT GCAAACCCAA CACTGACGGC ATCTCGTCTT TCGAGGACCT TCTGGCCAAC 
ATCGTGCTGC GTGTCTTCGT CTGGGCGGTT TCAGCAACAA CATGCTTCGG CAACATCTTT 
GTGATCTGCA TGCGCTCCTA CATCCGCTCC GAGAACAAGC TCCACGCCAT GTGCATCATC 
TCTTTATGCT GTGCAGACGG CCTGATGGGT GTTTATCTGT TCATGATCGG CGCGTATGAC 
CTGAAGTTTC GCGGCGAGTA CAACCGTCAC GCTCAGGCCG GGATGGACAG CGAGGCCTGT 
CAGGTGATCG GCTCTCTGGC CATGCTGTCC ACCGAGGTGT CAGTGCTGCT GCTCACGTAC 
CTCACGCTGG AGAAGTACAT CTGCATCGTG TACCCGTTCC GCTACCTGAC GCTCGGCCGC 
CGCCGCACTG TCACCATCCT CGTGGTCATC TGGGTTCTGG GCTTCATCAT CGCCTTCCTG 
CCTCTGCTCT TTAAAGGCGT TTTCAGAAAC TTCTATGGAA CAAACGGAGT GTGTTTTCCT 
CTGCACTCGG AGCAGCCGGA GACACTCGGA GCTCAGATTT ACTCCATCGT CATCTTTCTA 
GGTCTCAATC TGGTGGCTTT TCTCATCATC GTGCTGTCTT ACGGAAGCAT GTTTTACAAT 
ATCCAGAGGA CAGGAACGCA GACCACCAAA TACAGCAACC ACATTAAGAA AGAGCTGACC 
ATCGCCAAGC GCTTCTTCTC CATCGTCATC ACCGACTCGC TGTGCTGGAT TCCCATCTTC 
ATCCTCAAGA CTCTCTCACT TATGGAGGTC GAGATCCCTG GAACCATCAG CTCATGGGTG 
GTCATCTTCA TTCTGCCCAT CAACAGCGCT CTGAACCCCA TCCTGTATAC ACTGACCACG 
AGGCCCTTTA AAGAGACTCT TCTCCAGGTC TGGTCAAACT ACAGACAAAG GAGACCCCTG 
TTCAGCAGCC GGCCGCCGCA TCTGCCCTCC TTCACCTGGC AGGAGATGTG GCCCCTGCAG 
GAAAACAGCC AGACCCTGTG CTCGCACCCA TCTGACATCT GCAACACCAC ACACTTACTG 
CCTGTGGAGG CGTCCAACGG CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177863.1
CDS	32..2278
Translation	MPNFFLPSVFFSSEVVLTMFNVSEDQPYCPLGYFPCGNLSTCLPQVLHCNGVDDCGNQADEENCGDNNGWPHLFDNYFGIPSNNLGNKSDACLLGTVPAECQCRDLELDCDGAHFKDVPMVSINVTMMSLQRNGLRKLNADMFLKYQSLQKLYLQHNRIKSVHPQAFRGLYNLTRLYLSYNRITTLLPDVFQDLHKLEWLILENNSLHHISSLTFSGLRSLVLLVLLNNALTKLDDICLEMPRLNWLDIEGNKMETVGNVTFRSCNMLTVLVLQRNRISRIHAQAFSLLRKLGELDLSSNRIEAIPPDLFVNLGDLLQLNISYNPIMNLRVDHFDKLHKLKSLSIEGIEIGNIHRRMFEPLKNLTHIYFKKFQYCGYAPYVRSCKPNTDGISSFEDLLANIVLRVFVWAVSATTCFGNIFVICMRSYIRSENKLHAMCIISLCCADGLMGVYLFMIGAYDLKFRGEYNRHAQAGMDSEACQVIGSLAMLSTEVSVLLLTYLTLEKYICIVYPFRYLTLGRRRTVTILVVIWVLGFIIAFLPLLFKGVFRNFYGTNGVCFPLHSEQPETLGAQIYSIVIFLGLNLVAFLIIVLSYGSMFYNIQRTGTQTTKYSNHIKKELTIAKRFFSIVITDSLCWIPIFILKTLSLMEVEIPGTISSWVVIFILPINSALNPILYTLTTRPFKETLLQVWSNYRQRRPLFSSRPPHLPSFTWQEMWPLQENSQTLCSHPSDICNTTHLLPVEASNGT

Target ORF information:

RefSeq Version	NM_001190934.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio relaxin family peptide receptor 1 (rxfp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190934.1

ORF Insert Sequence:

ATGCCTAATT TCTTTCTCCC GTCTGTGTTT TTCTCTTCTG AAGTGGTGCT GACCATGTTT 
AATGTATCAG AGGATCAGCC CTACTGCCCG CTGGGATATT TCCCCTGTGG AAACCTCAGC 
ACCTGCCTTC CTCAAGTGCT GCACTGCAAC GGCGTGGATG ACTGTGGAAA TCAGGCAGAC 
GAGGAAAACT GTGGAGACAA TAATGGCTGG CCACATCTCT TTGATAACTA TTTTGGCATC 
CCCAGTAATA ACCTTGGAAA CAAGTCAGAC GCCTGCTTGC TCGGGACCGT CCCGGCTGAG 
TGCCAGTGCA GAGATCTGGA GCTGGACTGT GATGGTGCAC ACTTTAAAGA CGTCCCAATG 
GTATCCATTA ATGTCACCAT GATGTCCCTG CAGAGGAACG GGCTGAGGAA ACTAAACGCT 
GACATGTTCT TGAAGTACCA GAGCCTTCAA AAGCTGTACC TCCAGCACAA CCGCATTAAA 
TCAGTTCACC CGCAGGCATT CAGAGGCCTT TACAATCTGA CCAGACTGTA TCTCAGCTAT 
AATCGAATCA CAACTCTTCT CCCTGATGTT TTCCAGGATT TACACAAACT AGAGTGGCTG 
ATTCTGGAGA ACAACAGCCT TCATCACATC TCCTCTTTGA CCTTCTCGGG TCTTCGCTCT 
CTTGTGCTGC TAGTGCTGTT GAACAATGCT CTCACAAAAC TGGATGATAT CTGCCTGGAG 
ATGCCACGGT TAAATTGGCT AGACATTGAG GGAAATAAGA TGGAGACGGT AGGAAACGTG 
ACCTTCAGGT CGTGTAACAT GCTAACAGTG CTGGTCCTGC AGCGGAACCG CATCAGTCGC 
ATCCACGCGC AAGCTTTCTC TCTGCTGCGG AAACTGGGAG AGCTGGATTT GTCCAGTAAC 
AGAATCGAAG CGATCCCGCC TGATCTTTTC GTCAATCTCG GAGACCTGCT GCAGCTGAAT 
ATTTCATATA ACCCGATCAT GAACCTGCGG GTGGACCACT TTGACAAGCT GCATAAGTTA 
AAGTCACTGA GCATTGAGGG AATCGAAATT GGGAACATTC ACAGACGGAT GTTTGAACCT 
CTCAAGAACC TGACTCACAT CTACTTTAAA AAGTTCCAGT ACTGTGGTTA TGCTCCTTAC 
GTCCGCAGCT GCAAACCCAA CACTGACGGC ATCTCGTCTT TCGAGGACCT TCTGGCCAAC 
ATCGTGCTGC GTGTCTTCGT CTGGGCGGTT TCAGCAACAA CATGCTTCGG CAACATCTTT 
GTGATCTGCA TGCGCTCCTA CATCCGCTCC GAGAACAAGC TCCACGCCAT GTGCATCATC 
TCTTTATGCT GTGCAGACGG CCTGATGGGT GTTTATCTGT TCATGATCGG CGCGTATGAC 
CTGAAGTTTC GCGGCGAGTA CAACCGTCAC GCTCAGGCCG GGATGGACAG CGAGGCCTGT 
CAGGTGATCG GCTCTCTGGC CATGCTGTCC ACCGAGGTGT CAGTGCTGCT GCTCACGTAC 
CTCACGCTGG AGAAGTACAT CTGCATCGTG TACCCGTTCC GCTACCTGAC GCTCGGCCGC 
CGCCGCACTG TCACCATCCT CGTGGTCATC TGGGTTCTGG GCTTCATCAT CGCCTTCCTG 
CCTCTGCTCT TTAAAGGCGT TTTCAGAAAC TTCTATGGAA CAAACGGAGT GTGTTTTCCT 
CTGCACTCGG AGCAGCCGGA GACACTCGGA GCTCAGATTT ACTCCATCGT CATCTTTCTA 
GGTCTCAATC TGGTGGCTTT TCTCATCATC GTGCTGTCTT ACGGAAGCAT GTTTTACAAT 
ATCCAGAGGA CAGGAACGCA GACCACCAAA TACAGCAACC ACATTAAGAA AGAGCTGACC 
ATCGCCAAGC GCTTCTTCTC CATCGTCATC ACCGACTCGC TGTGCTGGAT TCCCATCTTC 
ATCCTCAAGA CTCTCTCACT TATGGAGGTC GAGATCCCTG GAACCATCAG CTCATGGGTG 
GTCATCTTCA TTCTGCCCAT CAACAGCGCT CTGAACCCCA TCCTGTATAC ACTGACCACG 
AGGCCCTTTA AAGAGACTCT TCTCCAGGTC TGGTCAAACT ACAGACAAAG GAGACCCCTG 
TTCAGCAGCC GGCCGCCGCA TCTGCCCTCC TTCACCTGGC AGGAGATGTG GCCCCTGCAG 
GAAAACAGCC AGACCCTGTG CTCGCACCCA TCTGACATCT GCAACACCAC ACACTTACTG 
CCTGTGGAGG CGTCCAACGG CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rxfp1 ( XM_009291265.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289540.1 Danio rerio relaxin/insulin-like family peptide receptor 1 (rxfp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa50039
Clone ID Related Accession (Same CDS sequence)	XM_009291265.2 , XM_009291265.3
Accession Version	XM_009291265.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	relaxin receptor 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291265.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTTC ACATCGCCAG CATCCTGATG TGCGGAGCCT CCGTGGTGCT GACCATGTTT 
AATGTATCAG AGGATCAGCC CTACTGCCCG CTGGGATATT TCCCCTGTGG AAACCTCAGC 
ACCTGCCTTC CTCAAGTGCT GCACTGCAAC GGCGTGGATG ACTGTGGAAA TCAGGCAGAC 
GAGGAAAACT GTGGAGACAA TAATGGCTGG CCACATCTCT TTGATAACTA TTTTGGCATC 
CCCAGTAATA ACCTTGGAAA CAAGTCAAAC GCCTGCTTGC TCGGGACCGT CCCGGCTGAG 
TGCCAGTGCA GAGATCTGGA GCTGGACTGT GATGGTGCAC ACTTTAAAGA CGTCCCAATG 
GTATCCATTA ATGTCACCAT GATGTCCCTG CAGAGGAACG GGCTGAGGAA ACTAAACGCT 
GACATGTTCT TGAAGTACCA GAGCCTTCAA AAGCTGTACC TCCAGCACAA CCGCATTAAA 
TCAGTTCACC CGCAGGCATT CAGAGGCCTT TACAATCTGA CCAGACTGTA TCTCAGCTAC 
AATCGGATCA CAACTCTTCT CCCTGATGTT TTCCAGGATT TACACAAACT AGAGTGGCTG 
ATTCTGGAGA ACAACAGCCT TCATCACATC TCCTCTTTGA CCTTCTCGGG TCTTCGCTCT 
CTTGTGCTGC TAGTGCTGTT GAACAATGCT CTCACAAAAC TGGATGATAT CTGCCTGGAG 
ATGCCACGGT TAAATTGGCT AGACATTGAG GGAAATAAGA TGGAGACGGT GGGAAACGTG 
ACCTTCAGGT CGTGTAACAT GCTAACAGTG CTGGTCCTGC AGCGGAACCG CATCAGTCGC 
ATCCACGCGC AAGCTTTCTC TCTGCTGCGG AAACTGGGAG AGCTGGATTT GTCCAGTAAC 
AGAATCGAAG CGATCCCGCC TGATCTATTC GTCAATCTCG GAGACCTGCT GCAGCTGAAT 
ATTTCATATA ACCCGATCAT GAACCTGCGG GTGGACCACT TTGACAAGCT GCATAAGTTA 
AAGTCACTGA GCATTGAGGG AATCGAAATT GGGAACATTC ACAGACGGAT GTTTGAACCT 
CTCAAGAACC TGACTCACAT CTACTTTAAA AAGTTCCAGT ACTGTGGTTA TGCTCCTCAC 
GTCCGCAGCT GCAAACCCAA CACTGACGGC ATCTCGTCTT TCGAGGACCT TCTGGCCAAC 
ATCGTGCTGC GTGTCTTCGT CTGGGTGGTT TCAGCAACAA CATGCTTCGG CAACATCTTT 
GTGATCTGCA TGCGCTCCTA CATCCGCTCC GAGAACAAGC TCCACGCCAT GTGCATCATC 
TCTTTATGCT GTGCAGACGG CCTGATGGGT GTTTATCTGT TCATGATCGG CGCGTATGAC 
CTGAAGTTTC GCGGCGAGTA CAACCGTCAC GCTCAGGCCT GGATGGACAG TGAGGCCTGT 
CAGGTGATCG GCTCTCTGGC CATGCTGTCC ACCGAGGTGT CAGTGCTGCT GCTCACGTAC 
CTCACGCTGG AGAAGTACAT CTGCATCGTG TACCCATTCC GCTACCTGAC GCCCGGCCGC 
CGCCGCACTG TCACCATCCT CGTGGTCATC TGGGTTCTGG GCTTCATTAT CGCCTTCCTG 
CCTCTGCTCT TTAAAGGCGT TTTCAGAAAC TTCTATGGAA CAAACGGAGT GTGTTTTCCT 
CTGCACTCGG AGCAGCCGGA GACACTCGGA GCTCAGATTT ACTCCATCGT CATCTTTCTA 
GGTCTCAATC TGGTGGCTTT TCTCATCATC GTGCTGTCTT ACGGAAGCAT GTTTTACAAT 
ATCCAGAGGA CAGGAACGCA GACCACCAAA TACAGCAACC ACATTAAGAA AGAGCTGACC 
ATCGCCAAGC GCTTCTTCTC CATCGTCATC ACCGACTCGC TGTGCTGGAT TCCCATCTTC 
ATCCTCAAGA TTCTCTCACT TATGGAGGTC GAGATCCCTG GAACCATCAG CTCATGGGTG 
GTCATCTTCA TTCTGCCCAT CAACAGCGCT CTGAACCCCA TCCTGTATAC ACTGACCACG 
AGGCCCTTTA AAGAGACTCT TCTCCAGGTC TGGTCAAACT ACAGACAAAG GAGACCCCTG 
TTCAGCAGCC GGCCGCCGCA TCTGCCCTCC TTCACCTGGC AGGAGATGTG GCCCCTGCAG 
GAAAACAGCC AGACCCTGTG CTCGCACCCA TCTGACATCT GCAACACCAC ACACTTACTG 
CCTGTGGAGG CGTCCAACGG CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289540.1
CDS	115..2361
Translation	MQVHIASILMCGASVVLTMFNVSEDQPYCPLGYFPCGNLSTCLPQVLHCNGVDDCGNQADEENCGDNNGWPHLFDNYFGIPSNNLGNKSNACLLGTVPAECQCRDLELDCDGAHFKDVPMVSINVTMMSLQRNGLRKLNADMFLKYQSLQKLYLQHNRIKSVHPQAFRGLYNLTRLYLSYNRITTLLPDVFQDLHKLEWLILENNSLHHISSLTFSGLRSLVLLVLLNNALTKLDDICLEMPRLNWLDIEGNKMETVGNVTFRSCNMLTVLVLQRNRISRIHAQAFSLLRKLGELDLSSNRIEAIPPDLFVNLGDLLQLNISYNPIMNLRVDHFDKLHKLKSLSIEGIEIGNIHRRMFEPLKNLTHIYFKKFQYCGYAPHVRSCKPNTDGISSFEDLLANIVLRVFVWVVSATTCFGNIFVICMRSYIRSENKLHAMCIISLCCADGLMGVYLFMIGAYDLKFRGEYNRHAQAWMDSEACQVIGSLAMLSTEVSVLLLTYLTLEKYICIVYPFRYLTPGRRRTVTILVVIWVLGFIIAFLPLLFKGVFRNFYGTNGVCFPLHSEQPETLGAQIYSIVIFLGLNLVAFLIIVLSYGSMFYNIQRTGTQTTKYSNHIKKELTIAKRFFSIVITDSLCWIPIFILKILSLMEVEIPGTISSWVVIFILPINSALNPILYTLTTRPFKETLLQVWSNYRQRRPLFSSRPPHLPSFTWQEMWPLQENSQTLCSHPSDICNTTHLLPVEASNGT

Target ORF information:

RefSeq Version	XM_009291265.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio relaxin/insulin-like family peptide receptor 1 (rxfp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291265.2

ORF Insert Sequence:

ATGCAAGTTC ACATCGCCAG CATCCTGATG TGCGGAGCCT CCGTGGTGCT GACCATGTTT 
AATGTATCAG AGGATCAGCC CTACTGCCCG CTGGGATATT TCCCCTGTGG AAACCTCAGC 
ACCTGCCTTC CTCAAGTGCT GCACTGCAAC GGCGTGGATG ACTGTGGAAA TCAGGCAGAC 
GAGGAAAACT GTGGAGACAA TAATGGCTGG CCACATCTCT TTGATAACTA TTTTGGCATC 
CCCAGTAATA ACCTTGGAAA CAAGTCAAAC GCCTGCTTGC TCGGGACCGT CCCGGCTGAG 
TGCCAGTGCA GAGATCTGGA GCTGGACTGT GATGGTGCAC ACTTTAAAGA CGTCCCAATG 
GTATCCATTA ATGTCACCAT GATGTCCCTG CAGAGGAACG GGCTGAGGAA ACTAAACGCT 
GACATGTTCT TGAAGTACCA GAGCCTTCAA AAGCTGTACC TCCAGCACAA CCGCATTAAA 
TCAGTTCACC CGCAGGCATT CAGAGGCCTT TACAATCTGA CCAGACTGTA TCTCAGCTAC 
AATCGGATCA CAACTCTTCT CCCTGATGTT TTCCAGGATT TACACAAACT AGAGTGGCTG 
ATTCTGGAGA ACAACAGCCT TCATCACATC TCCTCTTTGA CCTTCTCGGG TCTTCGCTCT 
CTTGTGCTGC TAGTGCTGTT GAACAATGCT CTCACAAAAC TGGATGATAT CTGCCTGGAG 
ATGCCACGGT TAAATTGGCT AGACATTGAG GGAAATAAGA TGGAGACGGT GGGAAACGTG 
ACCTTCAGGT CGTGTAACAT GCTAACAGTG CTGGTCCTGC AGCGGAACCG CATCAGTCGC 
ATCCACGCGC AAGCTTTCTC TCTGCTGCGG AAACTGGGAG AGCTGGATTT GTCCAGTAAC 
AGAATCGAAG CGATCCCGCC TGATCTATTC GTCAATCTCG GAGACCTGCT GCAGCTGAAT 
ATTTCATATA ACCCGATCAT GAACCTGCGG GTGGACCACT TTGACAAGCT GCATAAGTTA 
AAGTCACTGA GCATTGAGGG AATCGAAATT GGGAACATTC ACAGACGGAT GTTTGAACCT 
CTCAAGAACC TGACTCACAT CTACTTTAAA AAGTTCCAGT ACTGTGGTTA TGCTCCTCAC 
GTCCGCAGCT GCAAACCCAA CACTGACGGC ATCTCGTCTT TCGAGGACCT TCTGGCCAAC 
ATCGTGCTGC GTGTCTTCGT CTGGGTGGTT TCAGCAACAA CATGCTTCGG CAACATCTTT 
GTGATCTGCA TGCGCTCCTA CATCCGCTCC GAGAACAAGC TCCACGCCAT GTGCATCATC 
TCTTTATGCT GTGCAGACGG CCTGATGGGT GTTTATCTGT TCATGATCGG CGCGTATGAC 
CTGAAGTTTC GCGGCGAGTA CAACCGTCAC GCTCAGGCCT GGATGGACAG TGAGGCCTGT 
CAGGTGATCG GCTCTCTGGC CATGCTGTCC ACCGAGGTGT CAGTGCTGCT GCTCACGTAC 
CTCACGCTGG AGAAGTACAT CTGCATCGTG TACCCATTCC GCTACCTGAC GCCCGGCCGC 
CGCCGCACTG TCACCATCCT CGTGGTCATC TGGGTTCTGG GCTTCATTAT CGCCTTCCTG 
CCTCTGCTCT TTAAAGGCGT TTTCAGAAAC TTCTATGGAA CAAACGGAGT GTGTTTTCCT 
CTGCACTCGG AGCAGCCGGA GACACTCGGA GCTCAGATTT ACTCCATCGT CATCTTTCTA 
GGTCTCAATC TGGTGGCTTT TCTCATCATC GTGCTGTCTT ACGGAAGCAT GTTTTACAAT 
ATCCAGAGGA CAGGAACGCA GACCACCAAA TACAGCAACC ACATTAAGAA AGAGCTGACC 
ATCGCCAAGC GCTTCTTCTC CATCGTCATC ACCGACTCGC TGTGCTGGAT TCCCATCTTC 
ATCCTCAAGA TTCTCTCACT TATGGAGGTC GAGATCCCTG GAACCATCAG CTCATGGGTG 
GTCATCTTCA TTCTGCCCAT CAACAGCGCT CTGAACCCCA TCCTGTATAC ACTGACCACG 
AGGCCCTTTA AAGAGACTCT TCTCCAGGTC TGGTCAAACT ACAGACAAAG GAGACCCCTG 
TTCAGCAGCC GGCCGCCGCA TCTGCCCTCC TTCACCTGGC AGGAGATGTG GCCCCTGCAG 
GAAAACAGCC AGACCCTGTG CTCGCACCCA TCTGACATCT GCAACACCAC ACACTTACTG 
CCTGTGGAGG CGTCCAACGG CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rxfp1 ( XM_009291265.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289540.1 Danio rerio relaxin/insulin-like family peptide receptor 1 (rxfp1), transcript variant X1, mRNA.

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CloneID	ODa50039
Clone ID Related Accession (Same CDS sequence)	XM_009291265.2 , XM_009291265.3
Accession Version	XM_009291265.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	relaxin receptor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291265.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTTC ACATCGCCAG CATCCTGATG TGCGGAGCCT CCGTGGTGCT GACCATGTTT 
AATGTATCAG AGGATCAGCC CTACTGCCCG CTGGGATATT TCCCCTGTGG AAACCTCAGC 
ACCTGCCTTC CTCAAGTGCT GCACTGCAAC GGCGTGGATG ACTGTGGAAA TCAGGCAGAC 
GAGGAAAACT GTGGAGACAA TAATGGCTGG CCACATCTCT TTGATAACTA TTTTGGCATC 
CCCAGTAATA ACCTTGGAAA CAAGTCAAAC GCCTGCTTGC TCGGGACCGT CCCGGCTGAG 
TGCCAGTGCA GAGATCTGGA GCTGGACTGT GATGGTGCAC ACTTTAAAGA CGTCCCAATG 
GTATCCATTA ATGTCACCAT GATGTCCCTG CAGAGGAACG GGCTGAGGAA ACTAAACGCT 
GACATGTTCT TGAAGTACCA GAGCCTTCAA AAGCTGTACC TCCAGCACAA CCGCATTAAA 
TCAGTTCACC CGCAGGCATT CAGAGGCCTT TACAATCTGA CCAGACTGTA TCTCAGCTAC 
AATCGGATCA CAACTCTTCT CCCTGATGTT TTCCAGGATT TACACAAACT AGAGTGGCTG 
ATTCTGGAGA ACAACAGCCT TCATCACATC TCCTCTTTGA CCTTCTCGGG TCTTCGCTCT 
CTTGTGCTGC TAGTGCTGTT GAACAATGCT CTCACAAAAC TGGATGATAT CTGCCTGGAG 
ATGCCACGGT TAAATTGGCT AGACATTGAG GGAAATAAGA TGGAGACGGT GGGAAACGTG 
ACCTTCAGGT CGTGTAACAT GCTAACAGTG CTGGTCCTGC AGCGGAACCG CATCAGTCGC 
ATCCACGCGC AAGCTTTCTC TCTGCTGCGG AAACTGGGAG AGCTGGATTT GTCCAGTAAC 
AGAATCGAAG CGATCCCGCC TGATCTATTC GTCAATCTCG GAGACCTGCT GCAGCTGAAT 
ATTTCATATA ACCCGATCAT GAACCTGCGG GTGGACCACT TTGACAAGCT GCATAAGTTA 
AAGTCACTGA GCATTGAGGG AATCGAAATT GGGAACATTC ACAGACGGAT GTTTGAACCT 
CTCAAGAACC TGACTCACAT CTACTTTAAA AAGTTCCAGT ACTGTGGTTA TGCTCCTCAC 
GTCCGCAGCT GCAAACCCAA CACTGACGGC ATCTCGTCTT TCGAGGACCT TCTGGCCAAC 
ATCGTGCTGC GTGTCTTCGT CTGGGTGGTT TCAGCAACAA CATGCTTCGG CAACATCTTT 
GTGATCTGCA TGCGCTCCTA CATCCGCTCC GAGAACAAGC TCCACGCCAT GTGCATCATC 
TCTTTATGCT GTGCAGACGG CCTGATGGGT GTTTATCTGT TCATGATCGG CGCGTATGAC 
CTGAAGTTTC GCGGCGAGTA CAACCGTCAC GCTCAGGCCT GGATGGACAG TGAGGCCTGT 
CAGGTGATCG GCTCTCTGGC CATGCTGTCC ACCGAGGTGT CAGTGCTGCT GCTCACGTAC 
CTCACGCTGG AGAAGTACAT CTGCATCGTG TACCCATTCC GCTACCTGAC GCCCGGCCGC 
CGCCGCACTG TCACCATCCT CGTGGTCATC TGGGTTCTGG GCTTCATTAT CGCCTTCCTG 
CCTCTGCTCT TTAAAGGCGT TTTCAGAAAC TTCTATGGAA CAAACGGAGT GTGTTTTCCT 
CTGCACTCGG AGCAGCCGGA GACACTCGGA GCTCAGATTT ACTCCATCGT CATCTTTCTA 
GGTCTCAATC TGGTGGCTTT TCTCATCATC GTGCTGTCTT ACGGAAGCAT GTTTTACAAT 
ATCCAGAGGA CAGGAACGCA GACCACCAAA TACAGCAACC ACATTAAGAA AGAGCTGACC 
ATCGCCAAGC GCTTCTTCTC CATCGTCATC ACCGACTCGC TGTGCTGGAT TCCCATCTTC 
ATCCTCAAGA TTCTCTCACT TATGGAGGTC GAGATCCCTG GAACCATCAG CTCATGGGTG 
GTCATCTTCA TTCTGCCCAT CAACAGCGCT CTGAACCCCA TCCTGTATAC ACTGACCACG 
AGGCCCTTTA AAGAGACTCT TCTCCAGGTC TGGTCAAACT ACAGACAAAG GAGACCCCTG 
TTCAGCAGCC GGCCGCCGCA TCTGCCCTCC TTCACCTGGC AGGAGATGTG GCCCCTGCAG 
GAAAACAGCC AGACCCTGTG CTCGCACCCA TCTGACATCT GCAACACCAC ACACTTACTG 
CCTGTGGAGG CGTCCAACGG CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289540.1
CDS	184..2430
Translation	MQVHIASILMCGASVVLTMFNVSEDQPYCPLGYFPCGNLSTCLPQVLHCNGVDDCGNQADEENCGDNNGWPHLFDNYFGIPSNNLGNKSNACLLGTVPAECQCRDLELDCDGAHFKDVPMVSINVTMMSLQRNGLRKLNADMFLKYQSLQKLYLQHNRIKSVHPQAFRGLYNLTRLYLSYNRITTLLPDVFQDLHKLEWLILENNSLHHISSLTFSGLRSLVLLVLLNNALTKLDDICLEMPRLNWLDIEGNKMETVGNVTFRSCNMLTVLVLQRNRISRIHAQAFSLLRKLGELDLSSNRIEAIPPDLFVNLGDLLQLNISYNPIMNLRVDHFDKLHKLKSLSIEGIEIGNIHRRMFEPLKNLTHIYFKKFQYCGYAPHVRSCKPNTDGISSFEDLLANIVLRVFVWVVSATTCFGNIFVICMRSYIRSENKLHAMCIISLCCADGLMGVYLFMIGAYDLKFRGEYNRHAQAWMDSEACQVIGSLAMLSTEVSVLLLTYLTLEKYICIVYPFRYLTPGRRRTVTILVVIWVLGFIIAFLPLLFKGVFRNFYGTNGVCFPLHSEQPETLGAQIYSIVIFLGLNLVAFLIIVLSYGSMFYNIQRTGTQTTKYSNHIKKELTIAKRFFSIVITDSLCWIPIFILKILSLMEVEIPGTISSWVVIFILPINSALNPILYTLTTRPFKETLLQVWSNYRQRRPLFSSRPPHLPSFTWQEMWPLQENSQTLCSHPSDICNTTHLLPVEASNGT

Target ORF information:

RefSeq Version	XM_009291265.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio relaxin/insulin-like family peptide receptor 1 (rxfp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291265.3

ORF Insert Sequence:

ATGCAAGTTC ACATCGCCAG CATCCTGATG TGCGGAGCCT CCGTGGTGCT GACCATGTTT 
AATGTATCAG AGGATCAGCC CTACTGCCCG CTGGGATATT TCCCCTGTGG AAACCTCAGC 
ACCTGCCTTC CTCAAGTGCT GCACTGCAAC GGCGTGGATG ACTGTGGAAA TCAGGCAGAC 
GAGGAAAACT GTGGAGACAA TAATGGCTGG CCACATCTCT TTGATAACTA TTTTGGCATC 
CCCAGTAATA ACCTTGGAAA CAAGTCAAAC GCCTGCTTGC TCGGGACCGT CCCGGCTGAG 
TGCCAGTGCA GAGATCTGGA GCTGGACTGT GATGGTGCAC ACTTTAAAGA CGTCCCAATG 
GTATCCATTA ATGTCACCAT GATGTCCCTG CAGAGGAACG GGCTGAGGAA ACTAAACGCT 
GACATGTTCT TGAAGTACCA GAGCCTTCAA AAGCTGTACC TCCAGCACAA CCGCATTAAA 
TCAGTTCACC CGCAGGCATT CAGAGGCCTT TACAATCTGA CCAGACTGTA TCTCAGCTAC 
AATCGGATCA CAACTCTTCT CCCTGATGTT TTCCAGGATT TACACAAACT AGAGTGGCTG 
ATTCTGGAGA ACAACAGCCT TCATCACATC TCCTCTTTGA CCTTCTCGGG TCTTCGCTCT 
CTTGTGCTGC TAGTGCTGTT GAACAATGCT CTCACAAAAC TGGATGATAT CTGCCTGGAG 
ATGCCACGGT TAAATTGGCT AGACATTGAG GGAAATAAGA TGGAGACGGT GGGAAACGTG 
ACCTTCAGGT CGTGTAACAT GCTAACAGTG CTGGTCCTGC AGCGGAACCG CATCAGTCGC 
ATCCACGCGC AAGCTTTCTC TCTGCTGCGG AAACTGGGAG AGCTGGATTT GTCCAGTAAC 
AGAATCGAAG CGATCCCGCC TGATCTATTC GTCAATCTCG GAGACCTGCT GCAGCTGAAT 
ATTTCATATA ACCCGATCAT GAACCTGCGG GTGGACCACT TTGACAAGCT GCATAAGTTA 
AAGTCACTGA GCATTGAGGG AATCGAAATT GGGAACATTC ACAGACGGAT GTTTGAACCT 
CTCAAGAACC TGACTCACAT CTACTTTAAA AAGTTCCAGT ACTGTGGTTA TGCTCCTCAC 
GTCCGCAGCT GCAAACCCAA CACTGACGGC ATCTCGTCTT TCGAGGACCT TCTGGCCAAC 
ATCGTGCTGC GTGTCTTCGT CTGGGTGGTT TCAGCAACAA CATGCTTCGG CAACATCTTT 
GTGATCTGCA TGCGCTCCTA CATCCGCTCC GAGAACAAGC TCCACGCCAT GTGCATCATC 
TCTTTATGCT GTGCAGACGG CCTGATGGGT GTTTATCTGT TCATGATCGG CGCGTATGAC 
CTGAAGTTTC GCGGCGAGTA CAACCGTCAC GCTCAGGCCT GGATGGACAG TGAGGCCTGT 
CAGGTGATCG GCTCTCTGGC CATGCTGTCC ACCGAGGTGT CAGTGCTGCT GCTCACGTAC 
CTCACGCTGG AGAAGTACAT CTGCATCGTG TACCCATTCC GCTACCTGAC GCCCGGCCGC 
CGCCGCACTG TCACCATCCT CGTGGTCATC TGGGTTCTGG GCTTCATTAT CGCCTTCCTG 
CCTCTGCTCT TTAAAGGCGT TTTCAGAAAC TTCTATGGAA CAAACGGAGT GTGTTTTCCT 
CTGCACTCGG AGCAGCCGGA GACACTCGGA GCTCAGATTT ACTCCATCGT CATCTTTCTA 
GGTCTCAATC TGGTGGCTTT TCTCATCATC GTGCTGTCTT ACGGAAGCAT GTTTTACAAT 
ATCCAGAGGA CAGGAACGCA GACCACCAAA TACAGCAACC ACATTAAGAA AGAGCTGACC 
ATCGCCAAGC GCTTCTTCTC CATCGTCATC ACCGACTCGC TGTGCTGGAT TCCCATCTTC 
ATCCTCAAGA TTCTCTCACT TATGGAGGTC GAGATCCCTG GAACCATCAG CTCATGGGTG 
GTCATCTTCA TTCTGCCCAT CAACAGCGCT CTGAACCCCA TCCTGTATAC ACTGACCACG 
AGGCCCTTTA AAGAGACTCT TCTCCAGGTC TGGTCAAACT ACAGACAAAG GAGACCCCTG 
TTCAGCAGCC GGCCGCCGCA TCTGCCCTCC TTCACCTGGC AGGAGATGTG GCCCCTGCAG 
GAAAACAGCC AGACCCTGTG CTCGCACCCA TCTGACATCT GCAACACCAC ACACTTACTG 
CCTGTGGAGG CGTCCAACGG CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Japanese medaka as a model for studying the relaxin family genes involved in neuroendocrine regulation: Insights from the expression of fish-specific rln3 and insl5 and rxfp3/4-type receptor paralogues.
Molecular and cellular endocrinology4872-11(2019 05)
Alnafea H,Vahkal B,Zelmer CK,Yegorov S,Bogerd J,Good SV

Functional characterization of zebrafish orthologs of the human Beta 3-Glucosyltransferase B3GLCT gene mutated in Peters Plus Syndrome.
PloS one12(9)e0184903(2017)
Weh E,Takeuchi H,Muheisen S,Haltiwanger RS,Semina EV

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

New insights into ligand-receptor pairing and coevolution of relaxin family peptides and their receptors in teleosts.
International journal of evolutionary biology2012310278(2012)
Good S,Yegorov S,Martijn J,Franck J,Bogerd J

Characterization and developmental expression pattern of the relaxin receptor rxfp1 gene in zebrafish.
Development, growth & differentiation52(9)799-806(2010 Dec)
Donizetti A,Fiengo M,del Gaudio R,Di Giaimo R,Minucci S,Aniello F

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rxfp1 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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