BRAFLDRAFT_275724 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_275724
Entrez Gene ID	7217764
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002592942.1	XP_002592988.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_275724 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_275724 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb05631	XM_002592942.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_275724 ( XM_002592942.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002592988.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb05631
Clone ID Related Accession (Same CDS sequence)	XM_002592942.1
Accession Version	XM_002592942.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101409). COMPLETENESS: incomplete on the 5' end.

ATGACTGTGG CCATGCGTGG CAGCCCCGCA GGCGGGGACA ACCCCCAGAA TCCAGCCCCG 
GGGGGTACAC CTGCCAACCC TCCCCAGCAA CAACAACAAC AACAACAAGC AGGGACTCCG 
ACAGCACCCC CTGTAGAACC ACCCCCTGCA CCTGAGGGGG AGGGGGGTGA TGTGGTTGCG 
GAGGGGGTAG AGGAGCTTCC TGAGGGGGTG GAGCCAGACT TCCCCAATGC GGAGCTTGCT 
AAGCTGGATG ACATGATAAA CCGACCCCGC TGGGTGGTGC CTGTACTGCC CAAGGGGGAG 
CTGGAGGTTC TACTGGAGGC CTCCATAGAG CTGTGCAAGA AAAGCCTGGA CACGCGGAGC 
GAAGCTTGTC AGCGATTCTT CCGCGACGGT CTCACCATTT CCTTCACCAA AATCCTGACT 
GACGACGCTG TTAGTGGCTG GAAATTCGAG ATCCATAGAT GTATCTTTAA GAATGCGGAG 
AAGTTGATAG AGCTGTGTGT GACCAAGTTG TCCCAGGACT GGTTCCCTCT GCTGGACCTG 
TTAGCAATGG CCCTCAACCC ACACTGCAAA TTTCATATCT ACAATGGCAC AAGACCGTCT 
GAAACTGTGC CAGTTGGAAC ACAGTTGGCT GATGATGAAC TTTTTGCAAG GCCCCCAGAC 
CCAAGAAACC CAAAGGGTTG GCTAGTGGAT TTGATAAACC GGTTCGGCCA GTTGGAAGGA 
TTCAAGATCC TGAAGGAACG ATTCCTGGCT GGACATCTCA ATGTTCCTAT CATCGCTGCT 
TTAATCAAGC CGTGGGGTCA GTGCTACGAG CTCCTTACTC CGCACACTGT AGAGGAATAC 
TTCCAGCCCA TCATCGGCAT CGTCCCCACC TTCTTGGACG GGCTGACGGA CGAAGAGCTG 
AAGAAGGAAG CCAAGAATGA GGCCAAGAAC GACCAGCTGT CTCAGATCAT CAAGGCGCTG 
AAGTGTCTGG CCTCCCGAGT CCCTAACCAG GAGGAGACCA TGAAGAACCT GGAGATGTTC 
CGCCTCAAGA TGATTCTCAG ATTGCTCCAG ATCTCCTCCT TCAATGGTAA GATGAATGCG 
CTGAACGAGG TGAACAAAGT GATCGCCAGC GTGTCGTACT ACACCCACCG ACACAGTGGC 
ATGGAGGAGG ATGAGTGGCT CACAGCTGAG AGAATGGCGG AATGGATCCA GCAGAACAAC 
GTCCTGTCCA TTGTGCTCCG TGACAGCCTG CATCAGCCCC AGTATGTGGA GAAACTAGAG 
AAAATCCTGC GCTTTGTCAT CAAGGAGAAA GCTCTAACCA TGGGGGACCT GGACAACATC 
TGGGCTGCAC AGGCTGGGAA GCATGAGGCT ATAGTGAAGA ATGTCCACGA CTTGTTGGCC 
AAACTGGCCT GGGACTTCTC ACCTGAACAG CTCGACCATC TCTTTGACTG CTTCAAGGAG 
AGCTGGAAAG ATGCCAGTAA GAAGCAGCGA GAGAAGCTAC TGGAGCTGAT CCGACGGCTG 
GCAGAGGACG ACAAGGATGG AGTCATGGCC CACAAGGTGG TGTTAAACCT CCTGTGGAAC 
CTGGCACACA GTGAAGACGT CCCCACAGAC ATTATGGACC AGGCCCTGAG TGCACACATT 
AAGATCCTGG ACTACAGCTG TTCACAGGAC CGTGATGCCC AGAAGACCCA GTGGATCGAC 
CGCTTCGTCG ATGAACTCAA GAACGACCAG TGGGTTCTTC CTGCCCTCAA GCAAATCCGT 
GAAATCTGCC AACTCTTCGG CGAGGCTCCG CAAAATTACA ACCACGCCCA GCGCAGTCCG 
CACGTTTACT ATCGACATGA GGTCATAAAC CAGCTACAAC AGACTCATTC TCTGGTGACG 
CTCGTGGCGG AAAATCTCGC TTCTTACATG AACAAAGTGC GGGGAATGCT GAAGGAAAAC 
CCGGACATGG ACCCGAATGT TGTCCAGCCT AGCGAGAGGT ACACCCATTT ACAGCAGGTC 
CAGGAGAGAC TCAACTTTCT TAGGTTTTTA CTGAAGGACG GCCAGCTGTG GCTGTGTGCC 
CCTCAAGCCA AACAGATCTG GAACTGTCTG GCAGAGAATG CTGTATTTGT TAGTGACAGA 
GAGGCATGCT TCAAATGGTT CTCAAAGCTG ATGGGAGATG AGCCTGACCT TGACCCTGAG 
ATCAACAGAG ACTTCTTTGA AGGCAACGTT CTACAGCTGG ACCCGGCACT GCTTACGGAG 
AATGGCATTA AATGCTTTGA CCGGTTCTTC AAGGCAGTGA ACCTGAAGGA GAACAAGCTG 
GTGTGCAAGC GGAGAACGTA CATGATGGAC AACCTGGAGC TGATCGGGCT GGAGTACATC 
TGGAGAGTCA TCCTCCTGGG GCAGGACGAG ATCGCAGCCA AGGCCATCGA CCTACTGAGG 
GAGACTTACA CCAACCTGGG GCCACGGCTA CAGACTAACC AGGTTGACAT CCATGTAGAT 
TTCATCCAGT CCTGTTTTGA TCGTCTGAAA GCTGCGTACG ACACGATATG TATCCTAGAA 
AATGACAAGG ACAGTTTGGA GGACATCAGA ACGGAGGTCA CCCGTATGAT CCGTGTGCTG 
AGCGTGCTTC GGGAATACAT CAGCGAGTAC GATAACGACT TCACCGAGGA GAGAACCATC 
CTTCCAATGG CCAGAGCCTG CAGAGGAAAG CATGTGTCGT TGATTGTCCG TTTCCCAAAC 
CAGGGAAGAC AGGTGGAAGA CTTGGATGTC TGGTCCCACA CAAACGAGAC TGTAGGGGCA 
CTACGGAGAC ATATTCTTAC CAGAGTTAAA GCGAACAATG CCAACACCAA GCTGGACCTG 
TATGTGAACG GAGAACTGTT AGACCCAGCT GATGATAGGA AACTCATCTC ACAGGTGCAA 
CTGAGAGATA AAACATTGAT CACAGCGAAA CTGTGCCAGG TGGGTGCCAA CATGCCGTCC 
TCTCCTGACA GCTCGTCTGA CTCGTCAGTG GGTTCTCCCA GTAACATGTT TGATGGTCCC 
AATGTGGAGG CTGAGAGCTG TCTACCTGGG GTGTTGGCCG CGCAGCAGCA GCGTTATGCC 
CAGTTCCTGT TCCAGCTGTC TGACCTGGGC AGCTCCCTGG CCATCCCCCA GCTGAGAGAC 
GGGGCACGAT CGCTACTCAA GCTCATGCCT GCAGATACCC ACACAGTGGA TAAGCTGCGT 
GCCCTGTGTC TGGACCATGC TAAGCTGGGA GAACAGAGTC TGTCCCCTTC CCTGGAGTCT 
CTCTTCTTCT CACAGTCCCC GGCACAAGTA CTCTATCATC TAGAGGTTGT CTATGCATTG 
CTGATGCCAG CCAACCAGCC ATTGGGAGAA GACGCCTTGG AGTTTCAGAT TAACTTCCTG 
AAGAGTGGAG GCGTGTCCTG TGTGCTCAAC ATGCTGACCA AGAACAACTT TCTCCCAAAT 
GCTGACATGG ACACCAGAAG GTCAGCATAC CTGCAGGTTC TAAAGGTAGG GAAGCTGATG 
CTGACGACAG TTGGGTACGC CATGGTGCAG CACGTAGCAG AGGCGTGCCA GCCGGACAGC 
CCCCACTCCG GTACGGTGTC CGCCAGCGCC CATAACCACG CCATCGTGCT GCAGCAGGCC 
CTACACCACA TCCCCAACCC CAACGCTGAG TGCATGTTGA GGAACGTGGC CCTGCGTCTC 
GGGCAGCAGT GTGTCGAGGA TGCCATGACT ACCCTCCCCG ACATGCCAGT CATCAAGGCT 
ATCCAGAAGA TCTCCTGGGC AGTCGCCAGC GGTAGCATGC ATCTGGTGCA TGGGTCGAAT 
GAGGAGATAC ACAAGGCCTT TGATAAGCCA GGGCAGACCA AGCTACCTGA CAGTGAAGAC 
GTGCAGGTGT GTAAGGAGGC TCTGGAGGTC CTGACGGTGT CCTTGGCGCT GTGTCCTGTG 
GCTCTGGAGG CACTGAGTAA GGACAAGGTG TGGCAGTGTG TCATCATCGA CCTGCTGCTG 
CACTGTAAAA GCAGAGCGGT GCGAGTGTCT GCAGCAGAAC AGTTCCTCCT CATGGCCACC 
AAGTGCTGCA CCGGCCACAG GCCTCTGGTC TTCTTCATCA CTCTTCTGTT CACTGTTCTG 
TCGGGCACAG CCAAGGACCA CGCTAGAAGT TCCACAGAAT ATTTTCAGTT ACTTTGTAGG 
CTGCTAAACT ATGCATGTGC CAGCAATGCC CCCCTCCCCA CTGCTGAAGT TCTACTGAAC 
AATGAAATCC AGTGGCTAAA GAGAGTCAGG GACATGGTGC AACAGTCTGG GTTAGAAAAC 
GTTATCGACG AGGCCCTGCT AGAGGGCCAC CTGGGAGTCA CCTGTGAGCT ACTTAGTTTC 
CAACCAGCTG AGAAAAAATA CCACATAGGG TCCGAGAAGG GAGGGGCTAA TCTCATCAAG 
GAGCTGGTGG AAGAGTTCCT GTTTCCGGCG TCCAAAATCG TGTTACAGGC CAGAAAAAGC 
GGGGAGTACC CCAGCGAACA GGCGCTGCCC GTCTGCTCAA CGTCCAACAC GACAAACGCG 
GCCTTTGACC TGCTGGTGAC GCTGTGTACG GGGTGTGTTC CCAACCTGAA GCTGCTATCA 
GACATGCTCA CAGACATGTA CTACTCAGGT AAGGATGCGC CCCTGATCGA GTGGGAGTAC 
CTGCCCCCGG TGGGACCCAG ACCCGTGAAG GGGTTTGTGG GACTGAAGAA CGCCGGAGCC 
ACCTGCTACA TGAACTCTGT CATCCAACAG TTGTACATGA TCAACTCTGT GCGATCTGGC 
ATCCTGACAG CAGAGGGAGC TGCTAATGAT AACATGGACG AAGAACTGTC CGGGGACGAA 
AAGCAGGACA GTGAGAGTAA CATCGACCCG GGAGAAGAGA GAGAAAGTTT CCACATGGAT 
GACAAGCCTT CTGGGAAGCA GGAGGGGAGG AAGGAGTATG ACATGGGGGT TTTACGTCAG 
ATCCAGGCCA TCTTCGGCCA CCTAGCTGCT AGCAAGCTCC AGTATTATGT ACCAAGGGGA 
TTCTGGAAAC AATTTAGATT ATGGGGAGAA CCAGTCAACC TTAGAGAACA ACACGACGCT 
CTAGAGTTCT TCAACAGCCT AGTAGACAGT CTAGACGAAG CCTTGAAGAC GCTTGGGTAC 
ACACCTGTCC TGTCCAAGGT GCTGGGAGGA TCCTTTGCTG ACCAGAAGAT CTGCAAAGAC 
TGTCCTCACA GATACTCGAG AGAAGAGTCG TTCACGACCC TGAATGTAGA CATCAGGAAC 
CACAGTAACC TGCTGGACTC TCTGGAACAG TATGTGAAGG GAGACCTGCT GGAGGGGGCT 
AATGCCTACC ACTGTGAGAA ATGTAACAAG AAGGTTGACA CTGTGAAAAG AATGTGTATA 
AAGAAACTAC CCCAAGTTTT AGCCATTCAG CTGAAGAGGT TTGATTATGA CTGGGAAAGG 
GAATGTGCAA TCAAGTTCAA TGATTACTTT GAATTTCCTC GTGAGTTTGA CATGGAACCC 
TACACAGTTA AAGGACTAGC CAAAATTGAA GGGGAAGAGA TCCATGGAGA TGATGAGAGC 
CCAGACAATG AGGATGCCGA GCCACCCAGT ACCAAATACA GACTGACTGG CATCCTGGTA 
CACAGTGGAC AGGCTAGTGG GGGGCACTAC TACTCCTACA TCATACAGAG GTACTACAGG 
TCCCCAGAGG GCCAGCTGCG CTGGTACAAG TTTGACGATG GGGAGGTGAC AGAGTGTAAG 
ATGGACGATG ATGAGGAGAT CAAGAACCAG TGCTTCGGTG GGGAGTACAT GGGGGAGGTG 
TTCGACCACA TGTTAAAAAG AATGTCCTAC AGACGGCAGA AGAGGTGGTG GAACGCCTAC 
ATTCTGTTCT ACGAGCGCTA CGACGTAGGA GACATCGAAC ACGTGTCAAA AAGTATAGAA 
GAATTGTCAC TAGGTGAGCA AACAAACATA GTAAGAGTGG GCATGCCGCT GCCCATCGAG 
CGAGCGGTGA GGAAACAGAA CGTCCAGTTC ATGCACAACA AGATGCAGTA CAGCCAGGAG 
TACTTCCAGT TCATGCGCAA ACTCATCACC TGTAACGCTC ACTACATGAG CCTTCAGCCA 
GGCCAGGACG AGCTGAGCCG AGAGGTGGAG GAACTGGCCA TGTTGAGTAT CCAGCTGGCC 
TCCAAGTTCC TCTTCACCAC AGGGTTCCAC ACCAAGAAGA CACTCAGGGG CTCAGCGAAT 
GAGTGGTACG ACGCCCTGAG CGTACTGCTG CGACACAGTC GTACAGTCCG CAGCTGGTTT 
GCTCACAACG TGCTGTTCAG CCACCCCAGT CGTTTCTCCG AATACCTGCT CGAGTGCCCT 
AGTTCTGAGG TGAGAAATGC CTTTGCAAAG ATTATAGTGT TCCTGGCCCA TTTCTCAAGA 
GACGATGGTC CATGTCCGCC TCCCTTGCTG CCTGGAAAAC AAGTGATCGA CGCTAACGCC 
ACGTTGAGCG ACCACCTGCT GACAGCTGTC CTGGCTCTAC TGAAGAAGGA AGTATCCGAG 
CACGGCAGAC ATCTCACCCA GTACTTCCAC CTCTTCCTCA TGTATGCAAA CCTCGGCCTG 
CCAGAGAGAA CACAGCTGCT GAAGCTGAAC GTACCCGCCA TGTTCATGCT GGTTGCGCTG 
GATGAGGGGC CGGGACCCCC CATCAAGTAC CAGTACGCCG AGCTGGGCAA GCTGTACTCT 
GTTGTCTCGC TGCTGGTGCG CTGCTGTGAT GTCTCCAAGT ACATGCAGTC TTCTATATCT 
GGAAACCCTC CCCTAGCCAA CCCCCACGGT GACCCCGTGT GTCAGCAGCC CATCATGCCA 
CTACAACCTC AAGTGCACGA GATCCTGTTC GTCAGAACAA GCTACGTCAA GAAGTTGATA 
GAAGACTGCA ACAACTCTGA GGAGACTACA AAGTTGCTTC GGTTTTGTTG CTGGGAGAAC 
CCCCACTTCT CGTCGACCGT CCTGAGCGAG CTTCTGTGGC AGGTGGCGTA CTCGTACACG 
TACGAGCTCA GGCCGTACCT GGACCTGCTG CTGCAGATGC TGCTCATCGA AGACTCCTGG 
CAGAACCACA GAATACACAA CGCACTCAAA GGCATTCCAG ACGATCGGGA CGGTTTGTTC 
GACACCATTC AGCGCTCCAA AAACCACTAT CAGAAGCGGG CCTACCAGTG CATCAAGTTC 
ATGGTGGCAC TTTTCAGCAG CTGCATGCCA GCCCAGCAGA TGTTACAGAG TAACGGGGAC 
CTGAAACGCA AGTGGACCTG GGCTGTGGAG TGGCTGGGAG ATGAGCTAGA GAGGAGGCCT 
TACTCAGCCA ACACTCAGTA CACCTACAAC AACTGGTCCC CTCCCGCACA GTCCAACGAA 
ACTTCTAATG GGTACTTCCT GGAGAGGTCA CACAGTGCTC GTCTAACGCT GTCTAAGGCC 
TGTGAGCTCT GCCCAGAGGA GGAGCCAGAG GAGCAGGAGG TAGTGGATGA TGCAGACAGC 
TCGCCGCCAG ACACAGGCGT GACCACACAC TACTCCCAGC AGCAGATCCA CCATATCCAG 
CAGCAGCAGC ACCCAGCTAC AACACACCAT GGAGTCAGCA TTCCCCCTAA TAACCAGGCG 
AATCCCGGAA ACTACGTCCC TCCAGCCAAC ATCCACCACC AAAACAACAC CATCAACAAT 
GCCCTGACCG GCCAATCACG TCAGCCGGTC CAGTCACATG ACAACAACTC CGACAACGGC 
GAAGATAGCG ACAGCATTCA AGGTCGAAGC AACAATGAGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002592988.1
CDS	1..7725
Translation	MTVAMRGSPAGGDNPQNPAPGGTPANPPQQQQQQQQAGTPTAPPVEPPPAPEGEGGDVVAEGVEELPEGVEPDFPNAELAKLDDMINRPRWVVPVLPKGELEVLLEASIELCKKSLDTRSEACQRFFRDGLTISFTKILTDDAVSGWKFEIHRCIFKNAEKLIELCVTKLSQDWFPLLDLLAMALNPHCKFHIYNGTRPSETVPVGTQLADDELFARPPDPRNPKGWLVDLINRFGQLEGFKILKERFLAGHLNVPIIAALIKPWGQCYELLTPHTVEEYFQPIIGIVPTFLDGLTDEELKKEAKNEAKNDQLSQIIKALKCLASRVPNQEETMKNLEMFRLKMILRLLQISSFNGKMNALNEVNKVIASVSYYTHRHSGMEEDEWLTAERMAEWIQQNNVLSIVLRDSLHQPQYVEKLEKILRFVIKEKALTMGDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKESWKDASKKQREKLLELIRRLAEDDKDGVMAHKVVLNLLWNLAHSEDVPTDIMDQALSAHIKILDYSCSQDRDAQKTQWIDRFVDELKNDQWVLPALKQIREICQLFGEAPQNYNHAQRSPHVYYRHEVINQLQQTHSLVTLVAENLASYMNKVRGMLKENPDMDPNVVQPSERYTHLQQVQERLNFLRFLLKDGQLWLCAPQAKQIWNCLAENAVFVSDREACFKWFSKLMGDEPDLDPEINRDFFEGNVLQLDPALLTENGIKCFDRFFKAVNLKENKLVCKRRTYMMDNLELIGLEYIWRVILLGQDEIAAKAIDLLRETYTNLGPRLQTNQVDIHVDFIQSCFDRLKAAYDTICILENDKDSLEDIRTEVTRMIRVLSVLREYISEYDNDFTEERTILPMARACRGKHVSLIVRFPNQGRQVEDLDVWSHTNETVGALRRHILTRVKANNANTKLDLYVNGELLDPADDRKLISQVQLRDKTLITAKLCQVGANMPSSPDSSSDSSVGSPSNMFDGPNVEAESCLPGVLAAQQQRYAQFLFQLSDLGSSLAIPQLRDGARSLLKLMPADTHTVDKLRALCLDHAKLGEQSLSPSLESLFFSQSPAQVLYHLEVVYALLMPANQPLGEDALEFQINFLKSGGVSCVLNMLTKNNFLPNADMDTRRSAYLQVLKVGKLMLTTVGYAMVQHVAEACQPDSPHSGTVSASAHNHAIVLQQALHHIPNPNAECMLRNVALRLGQQCVEDAMTTLPDMPVIKAIQKISWAVASGSMHLVHGSNEEIHKAFDKPGQTKLPDSEDVQVCKEALEVLTVSLALCPVALEALSKDKVWQCVIIDLLLHCKSRAVRVSAAEQFLLMATKCCTGHRPLVFFITLLFTVLSGTAKDHARSSTEYFQLLCRLLNYACASNAPLPTAEVLLNNEIQWLKRVRDMVQQSGLENVIDEALLEGHLGVTCELLSFQPAEKKYHIGSEKGGANLIKELVEEFLFPASKIVLQARKSGEYPSEQALPVCSTSNTTNAAFDLLVTLCTGCVPNLKLLSDMLTDMYYSGKDAPLIEWEYLPPVGPRPVKGFVGLKNAGATCYMNSVIQQLYMINSVRSGILTAEGAANDNMDEELSGDEKQDSESNIDPGEERESFHMDDKPSGKQEGRKEYDMGVLRQIQAIFGHLAASKLQYYVPRGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLDEALKTLGYTPVLSKVLGGSFADQKICKDCPHRYSREESFTTLNVDIRNHSNLLDSLEQYVKGDLLEGANAYHCEKCNKKVDTVKRMCIKKLPQVLAIQLKRFDYDWERECAIKFNDYFEFPREFDMEPYTVKGLAKIEGEEIHGDDESPDNEDAEPPSTKYRLTGILVHSGQASGGHYYSYIIQRYYRSPEGQLRWYKFDDGEVTECKMDDDEEIKNQCFGGEYMGEVFDHMLKRMSYRRQKRWWNAYILFYERYDVGDIEHVSKSIEELSLGEQTNIVRVGMPLPIERAVRKQNVQFMHNKMQYSQEYFQFMRKLITCNAHYMSLQPGQDELSREVEELAMLSIQLASKFLFTTGFHTKKTLRGSANEWYDALSVLLRHSRTVRSWFAHNVLFSHPSRFSEYLLECPSSEVRNAFAKIIVFLAHFSRDDGPCPPPLLPGKQVIDANATLSDHLLTAVLALLKKEVSEHGRHLTQYFHLFLMYANLGLPERTQLLKLNVPAMFMLVALDEGPGPPIKYQYAELGKLYSVVSLLVRCCDVSKYMQSSISGNPPLANPHGDPVCQQPIMPLQPQVHEILFVRTSYVKKLIEDCNNSEETTKLLRFCCWENPHFSSTVLSELLWQVAYSYTYELRPYLDLLLQMLLIEDSWQNHRIHNALKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKFMVALFSSCMPAQQMLQSNGDLKRKWTWAVEWLGDELERRPYSANTQYTYNNWSPPAQSNETSNGYFLERSHSARLTLSKACELCPEEEPEEQEVVDDADSSPPDTGVTTHYSQQQIHHIQQQQHPATTHHGVSIPPNNQANPGNYVPPANIHHQNNTINNALTGQSRQPVQSHDNNSDNGEDSDSIQGRSNNEL

Target ORF information:

RefSeq Version	XM_002592942.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002592942.1

ORF Insert Sequence:

ATGACTGTGG CCATGCGTGG CAGCCCCGCA GGCGGGGACA ACCCCCAGAA TCCAGCCCCG 
GGGGGTACAC CTGCCAACCC TCCCCAGCAA CAACAACAAC AACAACAAGC AGGGACTCCG 
ACAGCACCCC CTGTAGAACC ACCCCCTGCA CCTGAGGGGG AGGGGGGTGA TGTGGTTGCG 
GAGGGGGTAG AGGAGCTTCC TGAGGGGGTG GAGCCAGACT TCCCCAATGC GGAGCTTGCT 
AAGCTGGATG ACATGATAAA CCGACCCCGC TGGGTGGTGC CTGTACTGCC CAAGGGGGAG 
CTGGAGGTTC TACTGGAGGC CTCCATAGAG CTGTGCAAGA AAAGCCTGGA CACGCGGAGC 
GAAGCTTGTC AGCGATTCTT CCGCGACGGT CTCACCATTT CCTTCACCAA AATCCTGACT 
GACGACGCTG TTAGTGGCTG GAAATTCGAG ATCCATAGAT GTATCTTTAA GAATGCGGAG 
AAGTTGATAG AGCTGTGTGT GACCAAGTTG TCCCAGGACT GGTTCCCTCT GCTGGACCTG 
TTAGCAATGG CCCTCAACCC ACACTGCAAA TTTCATATCT ACAATGGCAC AAGACCGTCT 
GAAACTGTGC CAGTTGGAAC ACAGTTGGCT GATGATGAAC TTTTTGCAAG GCCCCCAGAC 
CCAAGAAACC CAAAGGGTTG GCTAGTGGAT TTGATAAACC GGTTCGGCCA GTTGGAAGGA 
TTCAAGATCC TGAAGGAACG ATTCCTGGCT GGACATCTCA ATGTTCCTAT CATCGCTGCT 
TTAATCAAGC CGTGGGGTCA GTGCTACGAG CTCCTTACTC CGCACACTGT AGAGGAATAC 
TTCCAGCCCA TCATCGGCAT CGTCCCCACC TTCTTGGACG GGCTGACGGA CGAAGAGCTG 
AAGAAGGAAG CCAAGAATGA GGCCAAGAAC GACCAGCTGT CTCAGATCAT CAAGGCGCTG 
AAGTGTCTGG CCTCCCGAGT CCCTAACCAG GAGGAGACCA TGAAGAACCT GGAGATGTTC 
CGCCTCAAGA TGATTCTCAG ATTGCTCCAG ATCTCCTCCT TCAATGGTAA GATGAATGCG 
CTGAACGAGG TGAACAAAGT GATCGCCAGC GTGTCGTACT ACACCCACCG ACACAGTGGC 
ATGGAGGAGG ATGAGTGGCT CACAGCTGAG AGAATGGCGG AATGGATCCA GCAGAACAAC 
GTCCTGTCCA TTGTGCTCCG TGACAGCCTG CATCAGCCCC AGTATGTGGA GAAACTAGAG 
AAAATCCTGC GCTTTGTCAT CAAGGAGAAA GCTCTAACCA TGGGGGACCT GGACAACATC 
TGGGCTGCAC AGGCTGGGAA GCATGAGGCT ATAGTGAAGA ATGTCCACGA CTTGTTGGCC 
AAACTGGCCT GGGACTTCTC ACCTGAACAG CTCGACCATC TCTTTGACTG CTTCAAGGAG 
AGCTGGAAAG ATGCCAGTAA GAAGCAGCGA GAGAAGCTAC TGGAGCTGAT CCGACGGCTG 
GCAGAGGACG ACAAGGATGG AGTCATGGCC CACAAGGTGG TGTTAAACCT CCTGTGGAAC 
CTGGCACACA GTGAAGACGT CCCCACAGAC ATTATGGACC AGGCCCTGAG TGCACACATT 
AAGATCCTGG ACTACAGCTG TTCACAGGAC CGTGATGCCC AGAAGACCCA GTGGATCGAC 
CGCTTCGTCG ATGAACTCAA GAACGACCAG TGGGTTCTTC CTGCCCTCAA GCAAATCCGT 
GAAATCTGCC AACTCTTCGG CGAGGCTCCG CAAAATTACA ACCACGCCCA GCGCAGTCCG 
CACGTTTACT ATCGACATGA GGTCATAAAC CAGCTACAAC AGACTCATTC TCTGGTGACG 
CTCGTGGCGG AAAATCTCGC TTCTTACATG AACAAAGTGC GGGGAATGCT GAAGGAAAAC 
CCGGACATGG ACCCGAATGT TGTCCAGCCT AGCGAGAGGT ACACCCATTT ACAGCAGGTC 
CAGGAGAGAC TCAACTTTCT TAGGTTTTTA CTGAAGGACG GCCAGCTGTG GCTGTGTGCC 
CCTCAAGCCA AACAGATCTG GAACTGTCTG GCAGAGAATG CTGTATTTGT TAGTGACAGA 
GAGGCATGCT TCAAATGGTT CTCAAAGCTG ATGGGAGATG AGCCTGACCT TGACCCTGAG 
ATCAACAGAG ACTTCTTTGA AGGCAACGTT CTACAGCTGG ACCCGGCACT GCTTACGGAG 
AATGGCATTA AATGCTTTGA CCGGTTCTTC AAGGCAGTGA ACCTGAAGGA GAACAAGCTG 
GTGTGCAAGC GGAGAACGTA CATGATGGAC AACCTGGAGC TGATCGGGCT GGAGTACATC 
TGGAGAGTCA TCCTCCTGGG GCAGGACGAG ATCGCAGCCA AGGCCATCGA CCTACTGAGG 
GAGACTTACA CCAACCTGGG GCCACGGCTA CAGACTAACC AGGTTGACAT CCATGTAGAT 
TTCATCCAGT CCTGTTTTGA TCGTCTGAAA GCTGCGTACG ACACGATATG TATCCTAGAA 
AATGACAAGG ACAGTTTGGA GGACATCAGA ACGGAGGTCA CCCGTATGAT CCGTGTGCTG 
AGCGTGCTTC GGGAATACAT CAGCGAGTAC GATAACGACT TCACCGAGGA GAGAACCATC 
CTTCCAATGG CCAGAGCCTG CAGAGGAAAG CATGTGTCGT TGATTGTCCG TTTCCCAAAC 
CAGGGAAGAC AGGTGGAAGA CTTGGATGTC TGGTCCCACA CAAACGAGAC TGTAGGGGCA 
CTACGGAGAC ATATTCTTAC CAGAGTTAAA GCGAACAATG CCAACACCAA GCTGGACCTG 
TATGTGAACG GAGAACTGTT AGACCCAGCT GATGATAGGA AACTCATCTC ACAGGTGCAA 
CTGAGAGATA AAACATTGAT CACAGCGAAA CTGTGCCAGG TGGGTGCCAA CATGCCGTCC 
TCTCCTGACA GCTCGTCTGA CTCGTCAGTG GGTTCTCCCA GTAACATGTT TGATGGTCCC 
AATGTGGAGG CTGAGAGCTG TCTACCTGGG GTGTTGGCCG CGCAGCAGCA GCGTTATGCC 
CAGTTCCTGT TCCAGCTGTC TGACCTGGGC AGCTCCCTGG CCATCCCCCA GCTGAGAGAC 
GGGGCACGAT CGCTACTCAA GCTCATGCCT GCAGATACCC ACACAGTGGA TAAGCTGCGT 
GCCCTGTGTC TGGACCATGC TAAGCTGGGA GAACAGAGTC TGTCCCCTTC CCTGGAGTCT 
CTCTTCTTCT CACAGTCCCC GGCACAAGTA CTCTATCATC TAGAGGTTGT CTATGCATTG 
CTGATGCCAG CCAACCAGCC ATTGGGAGAA GACGCCTTGG AGTTTCAGAT TAACTTCCTG 
AAGAGTGGAG GCGTGTCCTG TGTGCTCAAC ATGCTGACCA AGAACAACTT TCTCCCAAAT 
GCTGACATGG ACACCAGAAG GTCAGCATAC CTGCAGGTTC TAAAGGTAGG GAAGCTGATG 
CTGACGACAG TTGGGTACGC CATGGTGCAG CACGTAGCAG AGGCGTGCCA GCCGGACAGC 
CCCCACTCCG GTACGGTGTC CGCCAGCGCC CATAACCACG CCATCGTGCT GCAGCAGGCC 
CTACACCACA TCCCCAACCC CAACGCTGAG TGCATGTTGA GGAACGTGGC CCTGCGTCTC 
GGGCAGCAGT GTGTCGAGGA TGCCATGACT ACCCTCCCCG ACATGCCAGT CATCAAGGCT 
ATCCAGAAGA TCTCCTGGGC AGTCGCCAGC GGTAGCATGC ATCTGGTGCA TGGGTCGAAT 
GAGGAGATAC ACAAGGCCTT TGATAAGCCA GGGCAGACCA AGCTACCTGA CAGTGAAGAC 
GTGCAGGTGT GTAAGGAGGC TCTGGAGGTC CTGACGGTGT CCTTGGCGCT GTGTCCTGTG 
GCTCTGGAGG CACTGAGTAA GGACAAGGTG TGGCAGTGTG TCATCATCGA CCTGCTGCTG 
CACTGTAAAA GCAGAGCGGT GCGAGTGTCT GCAGCAGAAC AGTTCCTCCT CATGGCCACC 
AAGTGCTGCA CCGGCCACAG GCCTCTGGTC TTCTTCATCA CTCTTCTGTT CACTGTTCTG 
TCGGGCACAG CCAAGGACCA CGCTAGAAGT TCCACAGAAT ATTTTCAGTT ACTTTGTAGG 
CTGCTAAACT ATGCATGTGC CAGCAATGCC CCCCTCCCCA CTGCTGAAGT TCTACTGAAC 
AATGAAATCC AGTGGCTAAA GAGAGTCAGG GACATGGTGC AACAGTCTGG GTTAGAAAAC 
GTTATCGACG AGGCCCTGCT AGAGGGCCAC CTGGGAGTCA CCTGTGAGCT ACTTAGTTTC 
CAACCAGCTG AGAAAAAATA CCACATAGGG TCCGAGAAGG GAGGGGCTAA TCTCATCAAG 
GAGCTGGTGG AAGAGTTCCT GTTTCCGGCG TCCAAAATCG TGTTACAGGC CAGAAAAAGC 
GGGGAGTACC CCAGCGAACA GGCGCTGCCC GTCTGCTCAA CGTCCAACAC GACAAACGCG 
GCCTTTGACC TGCTGGTGAC GCTGTGTACG GGGTGTGTTC CCAACCTGAA GCTGCTATCA 
GACATGCTCA CAGACATGTA CTACTCAGGT AAGGATGCGC CCCTGATCGA GTGGGAGTAC 
CTGCCCCCGG TGGGACCCAG ACCCGTGAAG GGGTTTGTGG GACTGAAGAA CGCCGGAGCC 
ACCTGCTACA TGAACTCTGT CATCCAACAG TTGTACATGA TCAACTCTGT GCGATCTGGC 
ATCCTGACAG CAGAGGGAGC TGCTAATGAT AACATGGACG AAGAACTGTC CGGGGACGAA 
AAGCAGGACA GTGAGAGTAA CATCGACCCG GGAGAAGAGA GAGAAAGTTT CCACATGGAT 
GACAAGCCTT CTGGGAAGCA GGAGGGGAGG AAGGAGTATG ACATGGGGGT TTTACGTCAG 
ATCCAGGCCA TCTTCGGCCA CCTAGCTGCT AGCAAGCTCC AGTATTATGT ACCAAGGGGA 
TTCTGGAAAC AATTTAGATT ATGGGGAGAA CCAGTCAACC TTAGAGAACA ACACGACGCT 
CTAGAGTTCT TCAACAGCCT AGTAGACAGT CTAGACGAAG CCTTGAAGAC GCTTGGGTAC 
ACACCTGTCC TGTCCAAGGT GCTGGGAGGA TCCTTTGCTG ACCAGAAGAT CTGCAAAGAC 
TGTCCTCACA GATACTCGAG AGAAGAGTCG TTCACGACCC TGAATGTAGA CATCAGGAAC 
CACAGTAACC TGCTGGACTC TCTGGAACAG TATGTGAAGG GAGACCTGCT GGAGGGGGCT 
AATGCCTACC ACTGTGAGAA ATGTAACAAG AAGGTTGACA CTGTGAAAAG AATGTGTATA 
AAGAAACTAC CCCAAGTTTT AGCCATTCAG CTGAAGAGGT TTGATTATGA CTGGGAAAGG 
GAATGTGCAA TCAAGTTCAA TGATTACTTT GAATTTCCTC GTGAGTTTGA CATGGAACCC 
TACACAGTTA AAGGACTAGC CAAAATTGAA GGGGAAGAGA TCCATGGAGA TGATGAGAGC 
CCAGACAATG AGGATGCCGA GCCACCCAGT ACCAAATACA GACTGACTGG CATCCTGGTA 
CACAGTGGAC AGGCTAGTGG GGGGCACTAC TACTCCTACA TCATACAGAG GTACTACAGG 
TCCCCAGAGG GCCAGCTGCG CTGGTACAAG TTTGACGATG GGGAGGTGAC AGAGTGTAAG 
ATGGACGATG ATGAGGAGAT CAAGAACCAG TGCTTCGGTG GGGAGTACAT GGGGGAGGTG 
TTCGACCACA TGTTAAAAAG AATGTCCTAC AGACGGCAGA AGAGGTGGTG GAACGCCTAC 
ATTCTGTTCT ACGAGCGCTA CGACGTAGGA GACATCGAAC ACGTGTCAAA AAGTATAGAA 
GAATTGTCAC TAGGTGAGCA AACAAACATA GTAAGAGTGG GCATGCCGCT GCCCATCGAG 
CGAGCGGTGA GGAAACAGAA CGTCCAGTTC ATGCACAACA AGATGCAGTA CAGCCAGGAG 
TACTTCCAGT TCATGCGCAA ACTCATCACC TGTAACGCTC ACTACATGAG CCTTCAGCCA 
GGCCAGGACG AGCTGAGCCG AGAGGTGGAG GAACTGGCCA TGTTGAGTAT CCAGCTGGCC 
TCCAAGTTCC TCTTCACCAC AGGGTTCCAC ACCAAGAAGA CACTCAGGGG CTCAGCGAAT 
GAGTGGTACG ACGCCCTGAG CGTACTGCTG CGACACAGTC GTACAGTCCG CAGCTGGTTT 
GCTCACAACG TGCTGTTCAG CCACCCCAGT CGTTTCTCCG AATACCTGCT CGAGTGCCCT 
AGTTCTGAGG TGAGAAATGC CTTTGCAAAG ATTATAGTGT TCCTGGCCCA TTTCTCAAGA 
GACGATGGTC CATGTCCGCC TCCCTTGCTG CCTGGAAAAC AAGTGATCGA CGCTAACGCC 
ACGTTGAGCG ACCACCTGCT GACAGCTGTC CTGGCTCTAC TGAAGAAGGA AGTATCCGAG 
CACGGCAGAC ATCTCACCCA GTACTTCCAC CTCTTCCTCA TGTATGCAAA CCTCGGCCTG 
CCAGAGAGAA CACAGCTGCT GAAGCTGAAC GTACCCGCCA TGTTCATGCT GGTTGCGCTG 
GATGAGGGGC CGGGACCCCC CATCAAGTAC CAGTACGCCG AGCTGGGCAA GCTGTACTCT 
GTTGTCTCGC TGCTGGTGCG CTGCTGTGAT GTCTCCAAGT ACATGCAGTC TTCTATATCT 
GGAAACCCTC CCCTAGCCAA CCCCCACGGT GACCCCGTGT GTCAGCAGCC CATCATGCCA 
CTACAACCTC AAGTGCACGA GATCCTGTTC GTCAGAACAA GCTACGTCAA GAAGTTGATA 
GAAGACTGCA ACAACTCTGA GGAGACTACA AAGTTGCTTC GGTTTTGTTG CTGGGAGAAC 
CCCCACTTCT CGTCGACCGT CCTGAGCGAG CTTCTGTGGC AGGTGGCGTA CTCGTACACG 
TACGAGCTCA GGCCGTACCT GGACCTGCTG CTGCAGATGC TGCTCATCGA AGACTCCTGG 
CAGAACCACA GAATACACAA CGCACTCAAA GGCATTCCAG ACGATCGGGA CGGTTTGTTC 
GACACCATTC AGCGCTCCAA AAACCACTAT CAGAAGCGGG CCTACCAGTG CATCAAGTTC 
ATGGTGGCAC TTTTCAGCAG CTGCATGCCA GCCCAGCAGA TGTTACAGAG TAACGGGGAC 
CTGAAACGCA AGTGGACCTG GGCTGTGGAG TGGCTGGGAG ATGAGCTAGA GAGGAGGCCT 
TACTCAGCCA ACACTCAGTA CACCTACAAC AACTGGTCCC CTCCCGCACA GTCCAACGAA 
ACTTCTAATG GGTACTTCCT GGAGAGGTCA CACAGTGCTC GTCTAACGCT GTCTAAGGCC 
TGTGAGCTCT GCCCAGAGGA GGAGCCAGAG GAGCAGGAGG TAGTGGATGA TGCAGACAGC 
TCGCCGCCAG ACACAGGCGT GACCACACAC TACTCCCAGC AGCAGATCCA CCATATCCAG 
CAGCAGCAGC ACCCAGCTAC AACACACCAT GGAGTCAGCA TTCCCCCTAA TAACCAGGCG 
AATCCCGGAA ACTACGTCCC TCCAGCCAAC ATCCACCACC AAAACAACAC CATCAACAAT 
GCCCTGACCG GCCAATCACG TCAGCCGGTC CAGTCACATG ACAACAACTC CGACAACGGC 
GAAGATAGCG ACAGCATTCA AGGTCGAAGC AACAATGAGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_275724 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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