BRAFLDRAFT_66799 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_66799
Entrez Gene ID	7217011
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002600250.1	XP_002600296.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_66799 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_66799 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb05217	XM_002600250.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_66799 ( XM_002600250.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002600296.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb05217
Clone ID Related Accession (Same CDS sequence)	XM_002600250.1
Accession Version	XM_002600250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101469). COMPLETENESS: incomplete on both ends.

ATGGACGATC ATTCGGATGG ACTGTTTCCT GCGATCGAGA AAAATCCAGC AGAAGACATG 
GCGACAGACT TGGAAAATTG GCAGAACGAC AGCAAAGAAA TATCATGTGA GGAAACAATT 
GCTGAGGACA CCTCACCCAA CAAGAAAAAC GTTCCACACG CAGAGCTAGT GGACAGGCAT 
CAGGAAGTCA CTATGGATGG ATCCCACATA TGCAAGGAGT GCGGGTATGT TGCTGATAGC 
ATGCCCAGCC TGGTCGTGCA TACAAGGAGA CATACAGACA GAAAACCCAA CAAGTGTGAC 
CAGTGTGACT ATTCTGCTAA ATGGAAATGG CAGTTAGACC ACCACATTGC AAAACACCAT 
GGAAAACCGT ACATGTGTGG GGAGTGCGGG TACAGGACAG GTAAAATGTG TATCTTGACT 
GTACATATGA GAAAACATAC TGGTGTTAAA CCTTACATGT GTGGGGATTG TGGCTATAGA 
GCAGCTTACA GGTCTTCCCT GTACCTACAT ATGAGTACAC ATATACACAA TGTAGGGACT 
AAATATTATA TGTGTAGGGA TTGCGATTAC AGAACTGCTC ACAAGTCTGC CCTATCCCAA 
CACATGAGAA CACATACAGG CGAGAGACCC TACAAATGTG ACCAGTGTGA CTATTCCGCT 
ACACAGAAAA ACAATTTGAA CCGACACAGG GCCAAGCACA TGGATGATAA ACCCTACAAA 
TGTGACCAGT GTGACTATTC TGCAGCAAGG AAAGATACTT TAGACCGACA CATGGCTTCC 
CACATAGGAG ATAACCCCAA CAAGTGTGAC GTTTCTTCGC TGAAAAAAAG TTCCTCCGTA 
CATTTGGGCA GACAAGCCGT TGAATACGTG TGTGAAGAAT GCAGTTACAG GGCATCTGGC 
AGGACTGACT TAACCAACCA CATGAGAAAA CATACAGGTG AGAAACCCTA CAAGTGTGAC 
CAATGTGACT ATTCCTCTGC ATGGAAAAAT GCTTTAGCCC AACACAAATT GTCTAAACAC 
ACTGGAGAAA AACTCTACAT GTGTGAAAAG TGCGGACACA GGACTGCTAA CGTGTCTGGC 
TTAACTAATC ACATGAAAAA ACATACCGGT GAGAAACCTT ACAAATGTGA CCAGTGCGAC 
TATTCTGCTG CACAGAAATC TACTTTAGAC CAACATATGG TTAGGCACAC TGGTGAAAAA 
CCCTACATGT GTGGGGAGTG CGGATTCAAA ACTGCACACA AGTTCTCCCT GTCCCTACAT 
ATGAGAACAC ATACAGGTGA GAAACCCTAC AAATGTGACC AGTGCGACTG TTCCTATACA 
GTGAAAGGAC ATTTGGACAG ACACATGGCT AAACACACCG GGGCCAAGCC TTACATGTGT 
GAGGAGTGCG GCTACAGGAC GGCTGATAAA TCCTGCCTGT CAAAACACAT GAAAACACAT 
ACCGAAATGG ACAAGCATTT CTCTGGACTG ATTCCTGTAG GAAACGAGAA AAATTTTGCA 
GAAGAAATGG CAACCGTACA TGAAAAATGG CAGAACGAGA ACCAAGGAAT GCCATGCAAG 
GAAACTCTTG CTGTAGAGTC CTCACCCGAC AAGAAAACCG TTTCACAGGA AGAGCTGATG 
GAAAAGCATC AAGAAGAAAG TTCGCACGTA TGCAGTCAAT GTGGGTATAT TGCTGATAGC 
ATGTCCAGCC TGGATGTGCA TACAAGGAGA CATACCGATC GAAAACCCAA CAAGTGTGAC 
CAGTGTGACT ATTCTGCGAA ATGGAAATGG CAGTTAGACC ACCACATTGC AAAACACCAT 
GAAAAACCGT ACATGTGCGG GGAGTGCGGG TACAGGACAG GTAAAATGCC TAAGTTAACT 
GAACATATGA GAACACACAT CGGTGCTAAG CGCGAGAAAC CCTACAAGTG TGACCAGTGT 
GACTATTCTG CTGCACAGAA AGGCACTTTA GACCGACACG TCATGGCTAA ACACGCTGGA 
GAAAAACCCT ACATGTGCGG AGAGTGTGGG TTCAGAACGG CTGATAGGTA TACACTAACC 
GTACATATGC GAAGACATAC AGGTGAGAAA CCCTACAAAT GTGACCAGTG CGACTATTCT 
GCTGCACAGA AAGAAACTTT CAACTATAAA TTACTCCCAG TATTTCTATT CGCTGGATTG 
TCGCCCGGGA TCAAGAAAAA TCCAGTAGGA GACATGACAG CAGACTTGGA AAAATTCCAC 
AACCAAGGAA TGCCATACGA GGAAACTCTT GCTGTAGAAT CCCTACCCGA TAAGAAAACC 
GCTCCCCAGA CAGAGCTGAC TGACATGCAT CAGGAAGACG CTACGGGTGG ATCCCACACG 
TGCAAGCAAT GTGGGTATGC TGCTGAAAAC ATGTCCAGCC TGGCCGTGCA TACCAGTAGA 
CACACCGATC GAAAACCCAA CAAGTGTGAC CAGTGTGACT ATTCTGCGAA GTGGAAATGG 
CAGTTAGACC ACCACATTGC AAAACACCAT GAAAAACCGT ACATGTGTGG GGAGTGCGGG 
TATAGGACAA ATATAATGTC TAACTTAACA GAACATATGA GAACGCATAC CGGTGAGAAA 
CCTTATATAT GTAGGGAGTG CGGATACAGA ACTGCTCACC AGTTCTACCT GTTGACACAT 
ATGAGAACGC ATACAGGTGA GAAACCCTAC AAATGTGATA GGTGTGACTA TTCGGCTGCA 
CAGAAAGGCA GTTTAAAACA ACACAAGGCT ATACACACTA GTGAAAAACC CTATAGCTAC 
ACGTGTGATG AGTGCGGATA CACGACTGCT CAAAGGTCTA AACTATCCCG ACATATGAGG 
AGACATACAG ATGAGAAACC CTACAAATGT GATGAGTGTG ACTATTCCGC TGCATGGAAA 
TCTTCTGTGG ACCGACACAT GGCTGATAAA CACAATGGAG AAAAGTCTTA CATGTGTGAG 
AAGTGTGGGT ACAGGACAGC TACAAAATCT CGCCTATCTC AACATATGAA AGTGCATACT 
GGCGAAAAGC CCTACAAATG TGATCAGTGT GACTTTTCTT CGCCATGGAA AAAGAGTTTA 
GGCACGCACA TGGCTAAGCA CACTGGTGAG AAACGCTACA TGTGCGGGGA GTGTGGGTAC 
AGGACGGCTG ATAGGTCCTG CCTGTCAAAA CATTTGAGAA CACATACAGG GGAAAGGCCC 
TACAAATGTG ACCAGTGCGA CTATTCTGCT GTGCAGAAGG GAAAGGACGA GCATTTCTCT 
AGGCTGATTC CTGCAGGAAT CGAGAAAAAT CCAACAGAAG AAATGGCAAC AGTACATGGA 
AAATTGCAGA ACGAGAACCA GGAAATGCCA TGCGAGCAAA CGCTTGATGT AGAGTCCTCA 
CCCGACAAGA AAACTGTTTC ACAGGCAGAG CTGGTGGACA AGCATCAACA AGAAGGTTCC 
CACATATGCA AGCAATGTGG GTATGTAGCC GATAGCATGT CCAGCCTGAA TGTGAATACA 
AGGAGACATA CCGATCGAAA ACCCAACAAA TGTGACCAGT GTGACTATTC TGCGAAGTGG 
AAATGGCAGT TAGACCACCA CATTGCAAAA TATCATGAAA AACCGTACAT GTGCGGGGAG 
TGCGGGTACA GGACAGATAA CATGCGTACG TTAACTGTCC ATATGAGAAA ACATACCGGT 
GAGAAACCTT ACGCGTGTAG CGATTGCGAA TTCAGAACTG CTCAAAAGTC CTCCCTGTCC 
TTACATATGA GAAAACATGC ATGTGTGAAA CCCTACAAAT GTGACCAGTG TGACTATTCT 
GCTGCCCAGA AAATTACTTT GATCCGACAC ATTGCCAAAC ATGAGAAACC CTACAAATGT 
GACCAGTGCG ACTATTCGTC TGCCTGGAAA TCTACTTTAG ACCGACACGT CATGGCTAAA 
CACACTGGTG AAAAACCCTT CATGTGTGGG GAGTGTGGGT ACAGGACGGC TGACAAGTCT 
AGCCTAACCG TACATATGAG AAAACATACA GGTGCTAAAC CATACTTGTG TATGGAGTGT 
GGATTCAGAA CTGCCCACAG CTCCTCACTG TCCTTACACA TGAGAACACA TACAGGTGAG 
AAACCCTACA AATGCGACCA GTGCGACTAT TCCGCTGCGC AAAAAATCAA TTTGGTCCGA 
CACATTGCCA AACATTGTGA GAAACCCTAC AAATGTGACC AGTGCGACTA TTCCGCTGCG 
CAGAAAATCA CTTTGAACCG ACACATGGTC AAACATAGTG AGAAACCCTA CAAATGTGAC 
CAGTGCGACT ATTCCGCTGC GCAGAAAATC ATTTTGAACC GACACATGGT CAAACATAGT 
GAGAAACTCT ACATGTCGGG AGAGAGCGAG TACAGGACGG CTGGAAGTGC CGATCTTTCC 
CGAGATATGA GAAGACATAC AGATGACAAA CCGTATAAAT GTGATCAGGG TGGCTATTCT 
TCTACAGGGA AATATGCTTT ATCCCGACAC ATTGCAAAGC ACACTGGTGA CAAAACGTAC 
GTGTGTGAAG AGTGCGGATA CAGAACGGTT AGCAGGTGTC GCCTATCCGA ACATGTGAAA 
ATACATGCAG GTGAGAAACC CTACAAATGT GACAAGTGTG AGTATTCTTC CGTAAGGAAA 
TCTACTTTAC ACCGACACAT GGCAACACAC GGCGGAGAAA AACCCTACAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002600296.1
CDS	1..4674
Translation	MDDHSDGLFPAIEKNPAEDMATDLENWQNDSKEISCEETIAEDTSPNKKNVPHAELVDRHQEVTMDGSHICKECGYVADSMPSLVVHTRRHTDRKPNKCDQCDYSAKWKWQLDHHIAKHHGKPYMCGECGYRTGKMCILTVHMRKHTGVKPYMCGDCGYRAAYRSSLYLHMSTHIHNVGTKYYMCRDCDYRTAHKSALSQHMRTHTGERPYKCDQCDYSATQKNNLNRHRAKHMDDKPYKCDQCDYSAARKDTLDRHMASHIGDNPNKCDVSSLKKSSSVHLGRQAVEYVCEECSYRASGRTDLTNHMRKHTGEKPYKCDQCDYSSAWKNALAQHKLSKHTGEKLYMCEKCGHRTANVSGLTNHMKKHTGEKPYKCDQCDYSAAQKSTLDQHMVRHTGEKPYMCGECGFKTAHKFSLSLHMRTHTGEKPYKCDQCDCSYTVKGHLDRHMAKHTGAKPYMCEECGYRTADKSCLSKHMKTHTEMDKHFSGLIPVGNEKNFAEEMATVHEKWQNENQGMPCKETLAVESSPDKKTVSQEELMEKHQEESSHVCSQCGYIADSMSSLDVHTRRHTDRKPNKCDQCDYSAKWKWQLDHHIAKHHEKPYMCGECGYRTGKMPKLTEHMRTHIGAKREKPYKCDQCDYSAAQKGTLDRHVMAKHAGEKPYMCGECGFRTADRYTLTVHMRRHTGEKPYKCDQCDYSAAQKETFNYKLLPVFLFAGLSPGIKKNPVGDMTADLEKFHNQGMPYEETLAVESLPDKKTAPQTELTDMHQEDATGGSHTCKQCGYAAENMSSLAVHTSRHTDRKPNKCDQCDYSAKWKWQLDHHIAKHHEKPYMCGECGYRTNIMSNLTEHMRTHTGEKPYICRECGYRTAHQFYLLTHMRTHTGEKPYKCDRCDYSAAQKGSLKQHKAIHTSEKPYSYTCDECGYTTAQRSKLSRHMRRHTDEKPYKCDECDYSAAWKSSVDRHMADKHNGEKSYMCEKCGYRTATKSRLSQHMKVHTGEKPYKCDQCDFSSPWKKSLGTHMAKHTGEKRYMCGECGYRTADRSCLSKHLRTHTGERPYKCDQCDYSAVQKGKDEHFSRLIPAGIEKNPTEEMATVHGKLQNENQEMPCEQTLDVESSPDKKTVSQAELVDKHQQEGSHICKQCGYVADSMSSLNVNTRRHTDRKPNKCDQCDYSAKWKWQLDHHIAKYHEKPYMCGECGYRTDNMRTLTVHMRKHTGEKPYACSDCEFRTAQKSSLSLHMRKHACVKPYKCDQCDYSAAQKITLIRHIAKHEKPYKCDQCDYSSAWKSTLDRHVMAKHTGEKPFMCGECGYRTADKSSLTVHMRKHTGAKPYLCMECGFRTAHSSSLSLHMRTHTGEKPYKCDQCDYSAAQKINLVRHIAKHCEKPYKCDQCDYSAAQKITLNRHMVKHSEKPYKCDQCDYSAAQKIILNRHMVKHSEKLYMSGESEYRTAGSADLSRDMRRHTDDKPYKCDQGGYSSTGKYALSRHIAKHTGDKTYVCEECGYRTVSRCRLSEHVKIHAGEKPYKCDKCEYSSVRKSTLHRHMATHGGEKPYM

Target ORF information:

RefSeq Version	XM_002600250.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002600250.1

ORF Insert Sequence:

ATGGACGATC ATTCGGATGG ACTGTTTCCT GCGATCGAGA AAAATCCAGC AGAAGACATG 
GCGACAGACT TGGAAAATTG GCAGAACGAC AGCAAAGAAA TATCATGTGA GGAAACAATT 
GCTGAGGACA CCTCACCCAA CAAGAAAAAC GTTCCACACG CAGAGCTAGT GGACAGGCAT 
CAGGAAGTCA CTATGGATGG ATCCCACATA TGCAAGGAGT GCGGGTATGT TGCTGATAGC 
ATGCCCAGCC TGGTCGTGCA TACAAGGAGA CATACAGACA GAAAACCCAA CAAGTGTGAC 
CAGTGTGACT ATTCTGCTAA ATGGAAATGG CAGTTAGACC ACCACATTGC AAAACACCAT 
GGAAAACCGT ACATGTGTGG GGAGTGCGGG TACAGGACAG GTAAAATGTG TATCTTGACT 
GTACATATGA GAAAACATAC TGGTGTTAAA CCTTACATGT GTGGGGATTG TGGCTATAGA 
GCAGCTTACA GGTCTTCCCT GTACCTACAT ATGAGTACAC ATATACACAA TGTAGGGACT 
AAATATTATA TGTGTAGGGA TTGCGATTAC AGAACTGCTC ACAAGTCTGC CCTATCCCAA 
CACATGAGAA CACATACAGG CGAGAGACCC TACAAATGTG ACCAGTGTGA CTATTCCGCT 
ACACAGAAAA ACAATTTGAA CCGACACAGG GCCAAGCACA TGGATGATAA ACCCTACAAA 
TGTGACCAGT GTGACTATTC TGCAGCAAGG AAAGATACTT TAGACCGACA CATGGCTTCC 
CACATAGGAG ATAACCCCAA CAAGTGTGAC GTTTCTTCGC TGAAAAAAAG TTCCTCCGTA 
CATTTGGGCA GACAAGCCGT TGAATACGTG TGTGAAGAAT GCAGTTACAG GGCATCTGGC 
AGGACTGACT TAACCAACCA CATGAGAAAA CATACAGGTG AGAAACCCTA CAAGTGTGAC 
CAATGTGACT ATTCCTCTGC ATGGAAAAAT GCTTTAGCCC AACACAAATT GTCTAAACAC 
ACTGGAGAAA AACTCTACAT GTGTGAAAAG TGCGGACACA GGACTGCTAA CGTGTCTGGC 
TTAACTAATC ACATGAAAAA ACATACCGGT GAGAAACCTT ACAAATGTGA CCAGTGCGAC 
TATTCTGCTG CACAGAAATC TACTTTAGAC CAACATATGG TTAGGCACAC TGGTGAAAAA 
CCCTACATGT GTGGGGAGTG CGGATTCAAA ACTGCACACA AGTTCTCCCT GTCCCTACAT 
ATGAGAACAC ATACAGGTGA GAAACCCTAC AAATGTGACC AGTGCGACTG TTCCTATACA 
GTGAAAGGAC ATTTGGACAG ACACATGGCT AAACACACCG GGGCCAAGCC TTACATGTGT 
GAGGAGTGCG GCTACAGGAC GGCTGATAAA TCCTGCCTGT CAAAACACAT GAAAACACAT 
ACCGAAATGG ACAAGCATTT CTCTGGACTG ATTCCTGTAG GAAACGAGAA AAATTTTGCA 
GAAGAAATGG CAACCGTACA TGAAAAATGG CAGAACGAGA ACCAAGGAAT GCCATGCAAG 
GAAACTCTTG CTGTAGAGTC CTCACCCGAC AAGAAAACCG TTTCACAGGA AGAGCTGATG 
GAAAAGCATC AAGAAGAAAG TTCGCACGTA TGCAGTCAAT GTGGGTATAT TGCTGATAGC 
ATGTCCAGCC TGGATGTGCA TACAAGGAGA CATACCGATC GAAAACCCAA CAAGTGTGAC 
CAGTGTGACT ATTCTGCGAA ATGGAAATGG CAGTTAGACC ACCACATTGC AAAACACCAT 
GAAAAACCGT ACATGTGCGG GGAGTGCGGG TACAGGACAG GTAAAATGCC TAAGTTAACT 
GAACATATGA GAACACACAT CGGTGCTAAG CGCGAGAAAC CCTACAAGTG TGACCAGTGT 
GACTATTCTG CTGCACAGAA AGGCACTTTA GACCGACACG TCATGGCTAA ACACGCTGGA 
GAAAAACCCT ACATGTGCGG AGAGTGTGGG TTCAGAACGG CTGATAGGTA TACACTAACC 
GTACATATGC GAAGACATAC AGGTGAGAAA CCCTACAAAT GTGACCAGTG CGACTATTCT 
GCTGCACAGA AAGAAACTTT CAACTATAAA TTACTCCCAG TATTTCTATT CGCTGGATTG 
TCGCCCGGGA TCAAGAAAAA TCCAGTAGGA GACATGACAG CAGACTTGGA AAAATTCCAC 
AACCAAGGAA TGCCATACGA GGAAACTCTT GCTGTAGAAT CCCTACCCGA TAAGAAAACC 
GCTCCCCAGA CAGAGCTGAC TGACATGCAT CAGGAAGACG CTACGGGTGG ATCCCACACG 
TGCAAGCAAT GTGGGTATGC TGCTGAAAAC ATGTCCAGCC TGGCCGTGCA TACCAGTAGA 
CACACCGATC GAAAACCCAA CAAGTGTGAC CAGTGTGACT ATTCTGCGAA GTGGAAATGG 
CAGTTAGACC ACCACATTGC AAAACACCAT GAAAAACCGT ACATGTGTGG GGAGTGCGGG 
TATAGGACAA ATATAATGTC TAACTTAACA GAACATATGA GAACGCATAC CGGTGAGAAA 
CCTTATATAT GTAGGGAGTG CGGATACAGA ACTGCTCACC AGTTCTACCT GTTGACACAT 
ATGAGAACGC ATACAGGTGA GAAACCCTAC AAATGTGATA GGTGTGACTA TTCGGCTGCA 
CAGAAAGGCA GTTTAAAACA ACACAAGGCT ATACACACTA GTGAAAAACC CTATAGCTAC 
ACGTGTGATG AGTGCGGATA CACGACTGCT CAAAGGTCTA AACTATCCCG ACATATGAGG 
AGACATACAG ATGAGAAACC CTACAAATGT GATGAGTGTG ACTATTCCGC TGCATGGAAA 
TCTTCTGTGG ACCGACACAT GGCTGATAAA CACAATGGAG AAAAGTCTTA CATGTGTGAG 
AAGTGTGGGT ACAGGACAGC TACAAAATCT CGCCTATCTC AACATATGAA AGTGCATACT 
GGCGAAAAGC CCTACAAATG TGATCAGTGT GACTTTTCTT CGCCATGGAA AAAGAGTTTA 
GGCACGCACA TGGCTAAGCA CACTGGTGAG AAACGCTACA TGTGCGGGGA GTGTGGGTAC 
AGGACGGCTG ATAGGTCCTG CCTGTCAAAA CATTTGAGAA CACATACAGG GGAAAGGCCC 
TACAAATGTG ACCAGTGCGA CTATTCTGCT GTGCAGAAGG GAAAGGACGA GCATTTCTCT 
AGGCTGATTC CTGCAGGAAT CGAGAAAAAT CCAACAGAAG AAATGGCAAC AGTACATGGA 
AAATTGCAGA ACGAGAACCA GGAAATGCCA TGCGAGCAAA CGCTTGATGT AGAGTCCTCA 
CCCGACAAGA AAACTGTTTC ACAGGCAGAG CTGGTGGACA AGCATCAACA AGAAGGTTCC 
CACATATGCA AGCAATGTGG GTATGTAGCC GATAGCATGT CCAGCCTGAA TGTGAATACA 
AGGAGACATA CCGATCGAAA ACCCAACAAA TGTGACCAGT GTGACTATTC TGCGAAGTGG 
AAATGGCAGT TAGACCACCA CATTGCAAAA TATCATGAAA AACCGTACAT GTGCGGGGAG 
TGCGGGTACA GGACAGATAA CATGCGTACG TTAACTGTCC ATATGAGAAA ACATACCGGT 
GAGAAACCTT ACGCGTGTAG CGATTGCGAA TTCAGAACTG CTCAAAAGTC CTCCCTGTCC 
TTACATATGA GAAAACATGC ATGTGTGAAA CCCTACAAAT GTGACCAGTG TGACTATTCT 
GCTGCCCAGA AAATTACTTT GATCCGACAC ATTGCCAAAC ATGAGAAACC CTACAAATGT 
GACCAGTGCG ACTATTCGTC TGCCTGGAAA TCTACTTTAG ACCGACACGT CATGGCTAAA 
CACACTGGTG AAAAACCCTT CATGTGTGGG GAGTGTGGGT ACAGGACGGC TGACAAGTCT 
AGCCTAACCG TACATATGAG AAAACATACA GGTGCTAAAC CATACTTGTG TATGGAGTGT 
GGATTCAGAA CTGCCCACAG CTCCTCACTG TCCTTACACA TGAGAACACA TACAGGTGAG 
AAACCCTACA AATGCGACCA GTGCGACTAT TCCGCTGCGC AAAAAATCAA TTTGGTCCGA 
CACATTGCCA AACATTGTGA GAAACCCTAC AAATGTGACC AGTGCGACTA TTCCGCTGCG 
CAGAAAATCA CTTTGAACCG ACACATGGTC AAACATAGTG AGAAACCCTA CAAATGTGAC 
CAGTGCGACT ATTCCGCTGC GCAGAAAATC ATTTTGAACC GACACATGGT CAAACATAGT 
GAGAAACTCT ACATGTCGGG AGAGAGCGAG TACAGGACGG CTGGAAGTGC CGATCTTTCC 
CGAGATATGA GAAGACATAC AGATGACAAA CCGTATAAAT GTGATCAGGG TGGCTATTCT 
TCTACAGGGA AATATGCTTT ATCCCGACAC ATTGCAAAGC ACACTGGTGA CAAAACGTAC 
GTGTGTGAAG AGTGCGGATA CAGAACGGTT AGCAGGTGTC GCCTATCCGA ACATGTGAAA 
ATACATGCAG GTGAGAAACC CTACAAATGT GACAAGTGTG AGTATTCTTC CGTAAGGAAA 
TCTACTTTAC ACCGACACAT GGCAACACAC GGCGGAGAAA AACCCTACAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_66799 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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