AFLA_023460 cDNA ORF clone, Aspergillus flavus NRRL3357

Gene
Clones
gRNAs

Summary

Gene Symbol	AFLA_023460
Entrez Gene ID	7915805
Full Name	alpha-1,3-glucan synthase Ags1
Gene Type	protein-coding
Organism	Aspergillus flavus NRRL3357

mRNA and Protein(s)

mRNA	Protein	Name
XM_002373816.1	XP_002373857.1	alpha-1,3-glucan synthase Ags1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome Sequence of Aspergillus flavus NRRL 3357, a Strain That Causes Aflatoxin Contamination of Food and Feed.
Nierman WC, Yu J, Fedorova-Abrams ND, Losada L, Cleveland TE, Bhatnagar D, Bennett JW, Dean R, Payne GA
Genome announcements3(2)(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AFLA_023460 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFLA_023460 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAk10204	XM_002373816.1 Latest version!	Aspergillus flavus NRRL3357 alpha-1,3-glucan synthase Ags1 (AFLA_023460), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFLA_023460 ( XM_002373816.1 ) cDNA ORF clone, Aspergillus flavus NRRL3357 -> XP_002373857.1 Aspergillus flavus NRRL3357 alpha-1,3-glucan synthase Ags1 (AFLA_023460), partial mRNA.

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CloneID	OAk10204
Clone ID Related Accession (Same CDS sequence)	XM_002373816.1
Accession Version	XM_002373816.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1522598400000
Organism	Aspergillus flavus NRRL3357
Product	alpha-1,3-glucan synthase Ags1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002477238). COMPLETENESS: incomplete on both ends.

ATGAAGTGGG CTTTCTCCAG TGCGGTGCTG GCGCTTTTCG CAACAACAGT AAAAGCCTGG 
CCTTACGAAG AATCTCTCTC CGCATACAAC CTTAACGAAA ACAAATCCGC GACCAACCCG 
GCTCAATATT GGGGAGAATG GCCGGACCAC AAGGGGAAAT ACTTCCCTTC TCCCGACAAT 
TGGCGATTTC CCGTCTATAC TCTGTTCATG GACCGCTTTG TCAACGGAGA CCCTACGAAC 
GACAACATCA ATGGAACCCT CTTCGAGCAC GATATCTCCT CGACACAAAT GCGCCACGGT 
GGAGATGTGG CTGGTCTAGT GGATACTTTG GATTATCTTC AGGGAATGGG TATCAAGGCC 
ATCTATCTCG CAGGAACCAT CTTGATGAAC CAGCCATGGG GCTCTGATGG TTATTCCGCT 
CTCGATACGA CACTGCTCGA TCAACATTTC GGTGACATTG CGACATGGCG TAATGCTATC 
GACGAGATTC ATAAGCGCGG GATGTATGTC ATCTTCGATA ACACGATTGC TACGATGGGT 
GATCTCATCG GCTTCGAGGG CCATCTGAAT GATACCACCC CGTTTTCGGT TAAGGAGCAT 
AAAGCACTTT GGAAGAGCAA TCGTCGCTAT GTGGATTTCG ATATAGGAAA CGACTATAAC 
CAGACATGCG ACTACCCCCG TTTCTGGTAC GAGGACGGTT ATCCAGTTCA ACAGTCTATG 
ACTGAAGGCC TTGTTGGTTG TTATGACAGC GACTTTGATC AATATGGTGA TATTGAGGCT 
TTCGGCGTGT TCCCTGATTG GCAACGTCAG CTAGCAAAAT TCGCCTCCGT CCAAGATCGT 
CTGCGAGAAT GGCACCCCTC GGTCCGGGAG CGGTTGATTC GTCATTCCTG TATGATTATT 
TACCAGTTGG ATATCGACGG TTTCCGTTAT GATAAGGCTA CTCAGTCGAC CGTGGATGCG 
CTAGGAGATA TGTCGATGGC TTATCGCGAA TGCGCCCGTG CCGTTGGCAA GGAGAATTTC 
TTCATCTCCG GTGAAATTAC TGGTGGTAAC ACTTTTGGTT CCATCTATTT GGGCCGAGGT 
AGACAGCCGA ACCAGTATCC TGAGACGGCG GAGAAGGCCA TGAAAATGAC CAACGAGTCC 
GAGTCGCAAT ACTTCCTGCG TGAAGCTGGA CATGAGGCGA TCGACGGTGC GGCCTTCCAC 
TATTCGACAT ATCGTGCCCT GACTCGGTTC TTGGGTATGG ACGGTAACTT GGCCGCCGGT 
TACGATGTAC CTGTGGATTG GGTCGATGCG TGGAATCTGA TGTTGCAGTC GAACGACTTC 
ATCAACCCTA ACACAGGCAA GTTTGATCCC CGCCATATGT TCGGCGCGAC CAACCAGGAT 
GTTTTCCGCT GGCCGACAGT CGAAAAGGGT GTGGAAAGAC AGTTGCTTGG GCTGTATATC 
ACTACTTTAC TTCTTCCGGG TATTCCCCTC CTCCTTTGGG GCGAGGAACA GGCATTCTAT 
ATCTTGGATG CGACGGCATC TAACTATATC TATGGCCGTC AAGCAATGTC CCCCGCGACT 
GCGTGGAGAG ACCACGGTTG CTTTTCCTTG GATTCCTCAC AGTATTACCA GTGGCCTATT 
CAGGCCGGTC GTGAGGGTTG CCATGACCCA ACTGCTGCGT ACGATCATCG TGATCCGGCC 
CACCCGGTGC GCAACATTAT CAAGCACATG TACCAGCTGC GCGAAGACTT CCCTGTTCTG 
AATGATGGCT ACTCCGTCCA GAAACTCTCG AACCTGACCG AGGAGGTCTT CTATCCGGGT 
TCCAACGGTA CCGCTACAGA AACGGGTTTG TGGTCTATCC TACGTGATGT CAATGCCGAT 
GTGCAGGACC TAGGCTCCGA CGCGAAGAAT CAACCGGTGT GGCTCGTCTA CCACAACACC 
AACCGTACAA TTGACTTCAA GTTCAACTGC AAGGACAACG AGACTGCACT AATCTCGCCC 
TTCGCCACCG GCACCAAAGT TCGAAATCTG TTCTATCCCT ATGACGAGCA CACCTTGATT 
GATGGCCCCG TCAAGCTTGG ACTGAACGGA TCTACCGAGC TCAATGGCTG CCTGGCCAAC 
ATGACATTGG ACGCCTATGA GTTCCGCGCC TACGTCCCCA GTGCACGTTT CACTAAGCCT 
CGTCCAATGA TCACCCAATT CACTCCCGGC CATGACGTCC CTGTTCGCTC CACGGTGGCT 
CCCAATCTGG ATGAAAGCGT GAAGATTGAG CTCTATTTCT CCGAAGAGAT GGACTGCGAT 
TCTGTGACCA AAGCGATTTC CATCAGCTCA TCTACGGAAT CTAAAAAGGT CCCGACGTTG 
GATGAGAAGA CTGTAGACTG CAAGGGAATT CCAGCAAGCA ACACCTCCTG GACTGGGCAG 
CTTCCTAGCG TCTTCATGTG GGCTGCCAAC CTGACGGGAG TGTATAACGG CATCCACCGA 
GTCACGGTTA AGAACGCTAG CAGTACTAAT GGAAACGCGA CAACAAACGC GGTCGACCAC 
TTCCTCTTCC GTATCGGACA AATCGATAAC CCCATGGTCT TTACATCGGC CAACTATTCG 
ACTAGTTTGC TCCACGAGGA ATCGAATGGC ACCCTATTCA TCCAGCACCA CGCAGCTGGT 
GCTGATAAGT GGCGTTATTC CACCAATTGG GGCACCACTT TCTCCGAGTG GAAGGATTAC 
ACAGGTGGTA ATGACACTAT CACGGAGTTA GAATGGTCTG GAACCAAGAA ACAGAGATGG 
AAGGGACACC ATGTGCGGGT CGAGTACTGG AGCAAATGGA CCGGTAGCAG CGATTACGTT 
CAGGAGGGCG ATGCTGGAGT GCATTCGAAT GTGCCACGCC GCTTCCCCCA TATCTTCTTC 
AACGGCCCTT ACAATCAGTA CGGATATGAC GGTGGTCTTG ATAACGTGGT GAGGCAGGAC 
TCCAAAGACG GACTCTGGAA ATATCACTTC ACGGCGGAGT GGCCGGCTCA AGCCCAGCTG 
AACATCTGGG GCATGAATCC GGATGGAAAG CCTGATCAAA GCTGGGTGCT GGGTGATGCC 
GATAATGATT CCGTTCTGGA TCGAATGCCA CCCTCCTCTC TCTCTGCAAC CTTGATTAAC 
ATCACCGAGC ATCCGCCTAA GCCATATCTG GCTTGGAATA TCTACATCAA CGATGCTACC 
ATGAAGTTCC AGCTCTTCCC TGTTGGGCAC CAGAACACGC AGATCGCCAT GTTCGTGCTC 
TTCTGGATCA TCCCTGTCAT CACCGGTGCA GCATGCGTCT ACATTTTCAT GAAGTCTTTC 
TATAAGGTCA AGTTCAACCA AATCGGTGTG AGTGAAAAAG CCACATTGAT CCCGTTGGCC 
TTGCGGAGAA AGTTCAAGAG GAATCGTGGT GGTGATGAGG AAAGGATGAA TCCCTTGATG 
CGTCTGGCCA ACAAGTCCGG TTTCCTGCAG ACCAACACCG CTATTGGCGG CGCTGCTTCT 
GGCAAGCGAC GCATGGTTCT TATCGCGACA ATGGAGTATG ATATCGAGGA TTGGCAGATT 
AAGATCAAGA TTGGTGGTCT TGGTGTCATG GCCCAGCTTA TGGGGAAAAC TCTCGGACAT 
CAGGACCTGA TCTGGGTTGT TCCCTGTGTC GGGGGAGTCG AATACCCAGT GGATAAACCC 
GCTGAGCCCA TGAATGTCAC GATTCTTGGC AACTCTTATG AGGTTCAGGT CCAGTACCAT 
GTCTTGAACA ATATCACCTA CGTTCTGCTA GACGCCCCTG TGTTCCGCCA GCAATCGAAG 
TCCGAACCCT ATCCAGCCCG TATGGATGAC CTCAACAGTG CTATCTACTA CTCTGCCTGG 
AATCAGTGTA TCGCTGAGGC CTGCAAGCGG TTCCCGATTG ACCTGTACCA TATCAACGAT 
TATCACGGTT CTCTAGCTCC GCTCTACCTT CTTCCCGATA CAGTACCGGC TTGTCTCTCC 
CTTCACAACG CTGAATTCCA GGGTCTCTGG CCAATGCGTA CACAGAAAGA AAAGGAGGAG 
GTGTGCTCCG TCTTTAACCT GGATATTGAT ATTGTCAGAC GTTATGTGCA GTTCGGTGAA 
GTTTTCAACT TGCTGCACTC AGGTGCTAGT TATCTTCGTG TTCACCAGCA GGGTTTCGGT 
GCCGTCGGTG TGTCCAAGAA GTACGGAAAG CGGTCCTACG CCCGTTATCC CATCTTCTGG 
GGTTTGAGGA AGGTCGGCAA CTTGCCTAAC CCTGATCCTT CGGATGTGGG AGAATGGAGT 
AAGGAAAAGG CTATTGGTAA CGCTGACGAG GTCCATGTGG ATCCCGACTA TGAGGCCGGC 
AGGGCAGACC TCAAACGCCA GGCTCAGGAA TGGGCTGGTC TTGATGTCAA CCCCGACGCT 
GATCTAATGG TGTTCGTTGG TCGTTGGTCC ATGCAGAAAG GTGTCGATTT AATCGCCGAT 
GTGATGCCAG CTGTTCTTGA AGCTCGCCCT AACGTGCAGG TAATCTGTGT TGGACCGGTT 
ATCGATCTTT ATGGTAAATT CGCCGCCCTG AAGTTGGACC ACATGATGAA GGTTTACCCT 
GGACGTGTGT TCTCGAGACC TGAATTCACC GCTCTTCCGC CTTATATCTT CTCTGGTGCT 
GAATTCGCGC TTATTCCTTC TCGTGACGAG CCCTTTGGTC TAGTCGCAGT CGAGTTCGGC 
CGTAAGGGAG CCTTAGGTAT CGGTGCCCGT GTCGGTGGTC TCGGTCAGAT GCCTGGATGG 
TGGTATAACG TCGAATCTAC TGCGACATCT CATCTTCTGT ACCAGTTCAA GCTTGCCATC 
GACGCCGCAC TCAACTCGAA ACAAGAGACC AGAGCCATGA TGCGTGCCCG TTCTGCTAAA 
CAGCGATTCC CCGTCGCCCA ATGGGTCGAG GACTTGGAAA TCCTGCAAAC CACCGCAATC 
CAAGTACACA ACAAGGAATT GGTCAAGCAC AACGGTCGTC CGTTCACTCC GACTGGAACG 
ACTACTCCTA GTGGCCTTAT GACTCAACCT GCGAGCCCCC TCGGGACCCC AGGAATGCAA 
ACTCCTCTTG CTCATTCTAG GGAAAGCAGC TACTCGAACC TCAACCGTCT AAGTGAATAC 
GTTACCCAGC CAAAGACCAG CTACAGCAGA GATCCCAGCC CTAGCGGCAC GGAGAAGCCG 
AAATCAGGAC TTCAGCGACA GCTTTCCCTT GGTGTTCGCT CTGGACCTGG TCATCAGAGC 
CGTCGTGGTC GCGCTCGCCA GCGTGACAGC ATCCCAGAAC ACGAAGACAC CCAGGAAGCT 
CACGGTGGCG CCATTACTGA TGTTGAGGAA GAAAGCAGTG ACGACGACAT TGTCAACCAT 
TACGCGGATG ACGAGTATAC TCTTACACCT GCCCAAGTCG AAGAAGGCCG TAGGTTGCAG 
GCCGCCCAGC AACAGGCTGG TATACGCATG CCGTTGAGTC CAGGTGGTAG ACGCTACAGC 
CAAGACTCGT TGCATCCGAG AAATGTCCAG CCTCCTTCGA GTCCCGGAAC ACCCCCAGCC 
GCTTCCCAGA GTCTCCTTCC TCCCCCTAGG CTCCTCGATC CCGGCAGTCG TCTCATCTAT 
CGTGATAAGC ACGGCGCTTC CCCTATCGGC TCGTTCTACG ATGATAACGG CTCCCGTATG 
AGTGGTAGCG ATGGCCCTCA CTCCAATGAC AGTGAAGACG ACGAGTTCCT CCTCGGAAAG 
GACTATGTCC CTCCCACCGG TCTCAAGAAG TGGATGCAGA TTCGCATCGG TGACTGGCCT 
ATCTACTCCT TGTTCCTCGC TTTAGGCCAA ATCATTGCTG CCAACTCGTA CCAGATCACA 
TTGCTCACGG GCGAAGTCGG TCAAACTGCC GAGAAACTGT ACGGAATTGC AACCACGTAT 
TTGATCACGT CTATTCTCTG GTGGCTTGTG TTCCGCTACT TCAAATCCGT CGTCTGTCTG 
TCTGCGCCAT GGTTCTTGTA CGGTATCGCC TTCATCTTCA TTGGATCCGC CCATTTTGAG 
AGCAACTCTT TCACTCGGGG ATGGATTCAA AATGTCGGTA GTGGGTTCTA CGCCGCGGCC 
TCGTCTAGTG GTTCTTTCTT CTTCGCGCTA AACTTCGGTG ATGAAGGTGG TGCACCTGTG 
GAAACATGGA TCTTCCGTGC ATGTCTCATT CAGGGTATCC AGTCCGCCTA TGTTATTGCT 
CTCTGGTACT GGGGTTCAAC CCTGTCGCAG GCACAAAGTG AGGGTCTCTT GACTCCTACA 
AACAATATCT CCAATTCTTG GAAGATGAGT GCCATCTGTT ACCCCATTGC CGCCGCCCTT 
TTCGGAATTG GTTTGCTCTT GACATTCGGC CTGCCCAACT ATTACCGTCA AACCCCTGGC 
AAGGTCGCTT CCTTCTACAA ATCCGTGTTC CGTCGTAAGA TCGTCCTCTG GAACTTTGTC 
GCGGTCATCC TTCAGAACTT CTTCCTCAGC GCCCCCTACG GCCGCAACTG GCAGTTCCTC 
TGGACATCCC ACCACGCACA TCACTGGCAA ATCGTCATCC TCTGTGTTGT TTTCTACGGC 
TTCGTATGGG CAGGTTTCCT ATTCGTCGTC AGTCGCTACT TCAAATCACA CAGCTGGTTC 
CTCCCCGTGT TCGCCTGTGG CCTCGGAGCT CCCCGCTGGG CACAAATCTG GTGGGGTGTG 
TCTGGCATTG GCTACTACCT CCCTTGGGTG ACAGGAGGAT ATACCGGCGG CGCGCTCGTC 
TCCCGAAGTG TCTGGCTCTG GCTCGGCGTG CTGGACTCGA TCCAGGGTCT CGGCTTCGGT 
ATCATCCTCT TGCAAACCCT GACTCGCATG CACATGCTTT TCTGTCTTGT TTGTTCTCAA 
GTCCTTGGTT CTATCGCTAC GATCTGCGCG AGAGCCTTCG CCCCTAATAA TGTGGGCCCA 
GGGCCGGTTT CGCCTGATCC TACCTTTGGT GGGAGTGCGG TTGCGAATGC CTGGTTCTGG 
GTTGCTCTGT TTTGTCAGTT GTTGGTCTGT GCCGGTTACA TCCTCTTCTT CCGGAAAGAA 
CAGCTGTCAA AGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002373857.1
CDS	1..7038
Translation	MKWAFSSAVLALFATTVKAWPYEESLSAYNLNENKSATNPAQYWGEWPDHKGKYFPSPDNWRFPVYTLFMDRFVNGDPTNDNINGTLFEHDISSTQMRHGGDVAGLVDTLDYLQGMGIKAIYLAGTILMNQPWGSDGYSALDTTLLDQHFGDIATWRNAIDEIHKRGMYVIFDNTIATMGDLIGFEGHLNDTTPFSVKEHKALWKSNRRYVDFDIGNDYNQTCDYPRFWYEDGYPVQQSMTEGLVGCYDSDFDQYGDIEAFGVFPDWQRQLAKFASVQDRLREWHPSVRERLIRHSCMIIYQLDIDGFRYDKATQSTVDALGDMSMAYRECARAVGKENFFISGEITGGNTFGSIYLGRGRQPNQYPETAEKAMKMTNESESQYFLREAGHEAIDGAAFHYSTYRALTRFLGMDGNLAAGYDVPVDWVDAWNLMLQSNDFINPNTGKFDPRHMFGATNQDVFRWPTVEKGVERQLLGLYITTLLLPGIPLLLWGEEQAFYILDATASNYIYGRQAMSPATAWRDHGCFSLDSSQYYQWPIQAGREGCHDPTAAYDHRDPAHPVRNIIKHMYQLREDFPVLNDGYSVQKLSNLTEEVFYPGSNGTATETGLWSILRDVNADVQDLGSDAKNQPVWLVYHNTNRTIDFKFNCKDNETALISPFATGTKVRNLFYPYDEHTLIDGPVKLGLNGSTELNGCLANMTLDAYEFRAYVPSARFTKPRPMITQFTPGHDVPVRSTVAPNLDESVKIELYFSEEMDCDSVTKAISISSSTESKKVPTLDEKTVDCKGIPASNTSWTGQLPSVFMWAANLTGVYNGIHRVTVKNASSTNGNATTNAVDHFLFRIGQIDNPMVFTSANYSTSLLHEESNGTLFIQHHAAGADKWRYSTNWGTTFSEWKDYTGGNDTITELEWSGTKKQRWKGHHVRVEYWSKWTGSSDYVQEGDAGVHSNVPRRFPHIFFNGPYNQYGYDGGLDNVVRQDSKDGLWKYHFTAEWPAQAQLNIWGMNPDGKPDQSWVLGDADNDSVLDRMPPSSLSATLINITEHPPKPYLAWNIYINDATMKFQLFPVGHQNTQIAMFVLFWIIPVITGAACVYIFMKSFYKVKFNQIGVSEKATLIPLALRRKFKRNRGGDEERMNPLMRLANKSGFLQTNTAIGGAASGKRRMVLIATMEYDIEDWQIKIKIGGLGVMAQLMGKTLGHQDLIWVVPCVGGVEYPVDKPAEPMNVTILGNSYEVQVQYHVLNNITYVLLDAPVFRQQSKSEPYPARMDDLNSAIYYSAWNQCIAEACKRFPIDLYHINDYHGSLAPLYLLPDTVPACLSLHNAEFQGLWPMRTQKEKEEVCSVFNLDIDIVRRYVQFGEVFNLLHSGASYLRVHQQGFGAVGVSKKYGKRSYARYPIFWGLRKVGNLPNPDPSDVGEWSKEKAIGNADEVHVDPDYEAGRADLKRQAQEWAGLDVNPDADLMVFVGRWSMQKGVDLIADVMPAVLEARPNVQVICVGPVIDLYGKFAALKLDHMMKVYPGRVFSRPEFTALPPYIFSGAEFALIPSRDEPFGLVAVEFGRKGALGIGARVGGLGQMPGWWYNVESTATSHLLYQFKLAIDAALNSKQETRAMMRARSAKQRFPVAQWVEDLEILQTTAIQVHNKELVKHNGRPFTPTGTTTPSGLMTQPASPLGTPGMQTPLAHSRESSYSNLNRLSEYVTQPKTSYSRDPSPSGTEKPKSGLQRQLSLGVRSGPGHQSRRGRARQRDSIPEHEDTQEAHGGAITDVEEESSDDDIVNHYADDEYTLTPAQVEEGRRLQAAQQQAGIRMPLSPGGRRYSQDSLHPRNVQPPSSPGTPPAASQSLLPPPRLLDPGSRLIYRDKHGASPIGSFYDDNGSRMSGSDGPHSNDSEDDEFLLGKDYVPPTGLKKWMQIRIGDWPIYSLFLALGQIIAANSYQITLLTGEVGQTAEKLYGIATTYLITSILWWLVFRYFKSVVCLSAPWFLYGIAFIFIGSAHFESNSFTRGWIQNVGSGFYAAASSSGSFFFALNFGDEGGAPVETWIFRACLIQGIQSAYVIALWYWGSTLSQAQSEGLLTPTNNISNSWKMSAICYPIAAALFGIGLLLTFGLPNYYRQTPGKVASFYKSVFRRKIVLWNFVAVILQNFFLSAPYGRNWQFLWTSHHAHHWQIVILCVVFYGFVWAGFLFVVSRYFKSHSWFLPVFACGLGAPRWAQIWWGVSGIGYYLPWVTGGYTGGALVSRSVWLWLGVLDSIQGLGFGIILLQTLTRMHMLFCLVCSQVLGSIATICARAFAPNNVGPGPVSPDPTFGGSAVANAWFWVALFCQLLVCAGYILFFRKEQLSKP

Target ORF information:

RefSeq Version	XM_002373816.1
Organism	Aspergillus flavus NRRL3357
Definition	Aspergillus flavus NRRL3357 alpha-1,3-glucan synthase Ags1 (AFLA_023460), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002373816.1

ORF Insert Sequence:

ATGAAGTGGG CTTTCTCCAG TGCGGTGCTG GCGCTTTTCG CAACAACAGT AAAAGCCTGG 
CCTTACGAAG AATCTCTCTC CGCATACAAC CTTAACGAAA ACAAATCCGC GACCAACCCG 
GCTCAATATT GGGGAGAATG GCCGGACCAC AAGGGGAAAT ACTTCCCTTC TCCCGACAAT 
TGGCGATTTC CCGTCTATAC TCTGTTCATG GACCGCTTTG TCAACGGAGA CCCTACGAAC 
GACAACATCA ATGGAACCCT CTTCGAGCAC GATATCTCCT CGACACAAAT GCGCCACGGT 
GGAGATGTGG CTGGTCTAGT GGATACTTTG GATTATCTTC AGGGAATGGG TATCAAGGCC 
ATCTATCTCG CAGGAACCAT CTTGATGAAC CAGCCATGGG GCTCTGATGG TTATTCCGCT 
CTCGATACGA CACTGCTCGA TCAACATTTC GGTGACATTG CGACATGGCG TAATGCTATC 
GACGAGATTC ATAAGCGCGG GATGTATGTC ATCTTCGATA ACACGATTGC TACGATGGGT 
GATCTCATCG GCTTCGAGGG CCATCTGAAT GATACCACCC CGTTTTCGGT TAAGGAGCAT 
AAAGCACTTT GGAAGAGCAA TCGTCGCTAT GTGGATTTCG ATATAGGAAA CGACTATAAC 
CAGACATGCG ACTACCCCCG TTTCTGGTAC GAGGACGGTT ATCCAGTTCA ACAGTCTATG 
ACTGAAGGCC TTGTTGGTTG TTATGACAGC GACTTTGATC AATATGGTGA TATTGAGGCT 
TTCGGCGTGT TCCCTGATTG GCAACGTCAG CTAGCAAAAT TCGCCTCCGT CCAAGATCGT 
CTGCGAGAAT GGCACCCCTC GGTCCGGGAG CGGTTGATTC GTCATTCCTG TATGATTATT 
TACCAGTTGG ATATCGACGG TTTCCGTTAT GATAAGGCTA CTCAGTCGAC CGTGGATGCG 
CTAGGAGATA TGTCGATGGC TTATCGCGAA TGCGCCCGTG CCGTTGGCAA GGAGAATTTC 
TTCATCTCCG GTGAAATTAC TGGTGGTAAC ACTTTTGGTT CCATCTATTT GGGCCGAGGT 
AGACAGCCGA ACCAGTATCC TGAGACGGCG GAGAAGGCCA TGAAAATGAC CAACGAGTCC 
GAGTCGCAAT ACTTCCTGCG TGAAGCTGGA CATGAGGCGA TCGACGGTGC GGCCTTCCAC 
TATTCGACAT ATCGTGCCCT GACTCGGTTC TTGGGTATGG ACGGTAACTT GGCCGCCGGT 
TACGATGTAC CTGTGGATTG GGTCGATGCG TGGAATCTGA TGTTGCAGTC GAACGACTTC 
ATCAACCCTA ACACAGGCAA GTTTGATCCC CGCCATATGT TCGGCGCGAC CAACCAGGAT 
GTTTTCCGCT GGCCGACAGT CGAAAAGGGT GTGGAAAGAC AGTTGCTTGG GCTGTATATC 
ACTACTTTAC TTCTTCCGGG TATTCCCCTC CTCCTTTGGG GCGAGGAACA GGCATTCTAT 
ATCTTGGATG CGACGGCATC TAACTATATC TATGGCCGTC AAGCAATGTC CCCCGCGACT 
GCGTGGAGAG ACCACGGTTG CTTTTCCTTG GATTCCTCAC AGTATTACCA GTGGCCTATT 
CAGGCCGGTC GTGAGGGTTG CCATGACCCA ACTGCTGCGT ACGATCATCG TGATCCGGCC 
CACCCGGTGC GCAACATTAT CAAGCACATG TACCAGCTGC GCGAAGACTT CCCTGTTCTG 
AATGATGGCT ACTCCGTCCA GAAACTCTCG AACCTGACCG AGGAGGTCTT CTATCCGGGT 
TCCAACGGTA CCGCTACAGA AACGGGTTTG TGGTCTATCC TACGTGATGT CAATGCCGAT 
GTGCAGGACC TAGGCTCCGA CGCGAAGAAT CAACCGGTGT GGCTCGTCTA CCACAACACC 
AACCGTACAA TTGACTTCAA GTTCAACTGC AAGGACAACG AGACTGCACT AATCTCGCCC 
TTCGCCACCG GCACCAAAGT TCGAAATCTG TTCTATCCCT ATGACGAGCA CACCTTGATT 
GATGGCCCCG TCAAGCTTGG ACTGAACGGA TCTACCGAGC TCAATGGCTG CCTGGCCAAC 
ATGACATTGG ACGCCTATGA GTTCCGCGCC TACGTCCCCA GTGCACGTTT CACTAAGCCT 
CGTCCAATGA TCACCCAATT CACTCCCGGC CATGACGTCC CTGTTCGCTC CACGGTGGCT 
CCCAATCTGG ATGAAAGCGT GAAGATTGAG CTCTATTTCT CCGAAGAGAT GGACTGCGAT 
TCTGTGACCA AAGCGATTTC CATCAGCTCA TCTACGGAAT CTAAAAAGGT CCCGACGTTG 
GATGAGAAGA CTGTAGACTG CAAGGGAATT CCAGCAAGCA ACACCTCCTG GACTGGGCAG 
CTTCCTAGCG TCTTCATGTG GGCTGCCAAC CTGACGGGAG TGTATAACGG CATCCACCGA 
GTCACGGTTA AGAACGCTAG CAGTACTAAT GGAAACGCGA CAACAAACGC GGTCGACCAC 
TTCCTCTTCC GTATCGGACA AATCGATAAC CCCATGGTCT TTACATCGGC CAACTATTCG 
ACTAGTTTGC TCCACGAGGA ATCGAATGGC ACCCTATTCA TCCAGCACCA CGCAGCTGGT 
GCTGATAAGT GGCGTTATTC CACCAATTGG GGCACCACTT TCTCCGAGTG GAAGGATTAC 
ACAGGTGGTA ATGACACTAT CACGGAGTTA GAATGGTCTG GAACCAAGAA ACAGAGATGG 
AAGGGACACC ATGTGCGGGT CGAGTACTGG AGCAAATGGA CCGGTAGCAG CGATTACGTT 
CAGGAGGGCG ATGCTGGAGT GCATTCGAAT GTGCCACGCC GCTTCCCCCA TATCTTCTTC 
AACGGCCCTT ACAATCAGTA CGGATATGAC GGTGGTCTTG ATAACGTGGT GAGGCAGGAC 
TCCAAAGACG GACTCTGGAA ATATCACTTC ACGGCGGAGT GGCCGGCTCA AGCCCAGCTG 
AACATCTGGG GCATGAATCC GGATGGAAAG CCTGATCAAA GCTGGGTGCT GGGTGATGCC 
GATAATGATT CCGTTCTGGA TCGAATGCCA CCCTCCTCTC TCTCTGCAAC CTTGATTAAC 
ATCACCGAGC ATCCGCCTAA GCCATATCTG GCTTGGAATA TCTACATCAA CGATGCTACC 
ATGAAGTTCC AGCTCTTCCC TGTTGGGCAC CAGAACACGC AGATCGCCAT GTTCGTGCTC 
TTCTGGATCA TCCCTGTCAT CACCGGTGCA GCATGCGTCT ACATTTTCAT GAAGTCTTTC 
TATAAGGTCA AGTTCAACCA AATCGGTGTG AGTGAAAAAG CCACATTGAT CCCGTTGGCC 
TTGCGGAGAA AGTTCAAGAG GAATCGTGGT GGTGATGAGG AAAGGATGAA TCCCTTGATG 
CGTCTGGCCA ACAAGTCCGG TTTCCTGCAG ACCAACACCG CTATTGGCGG CGCTGCTTCT 
GGCAAGCGAC GCATGGTTCT TATCGCGACA ATGGAGTATG ATATCGAGGA TTGGCAGATT 
AAGATCAAGA TTGGTGGTCT TGGTGTCATG GCCCAGCTTA TGGGGAAAAC TCTCGGACAT 
CAGGACCTGA TCTGGGTTGT TCCCTGTGTC GGGGGAGTCG AATACCCAGT GGATAAACCC 
GCTGAGCCCA TGAATGTCAC GATTCTTGGC AACTCTTATG AGGTTCAGGT CCAGTACCAT 
GTCTTGAACA ATATCACCTA CGTTCTGCTA GACGCCCCTG TGTTCCGCCA GCAATCGAAG 
TCCGAACCCT ATCCAGCCCG TATGGATGAC CTCAACAGTG CTATCTACTA CTCTGCCTGG 
AATCAGTGTA TCGCTGAGGC CTGCAAGCGG TTCCCGATTG ACCTGTACCA TATCAACGAT 
TATCACGGTT CTCTAGCTCC GCTCTACCTT CTTCCCGATA CAGTACCGGC TTGTCTCTCC 
CTTCACAACG CTGAATTCCA GGGTCTCTGG CCAATGCGTA CACAGAAAGA AAAGGAGGAG 
GTGTGCTCCG TCTTTAACCT GGATATTGAT ATTGTCAGAC GTTATGTGCA GTTCGGTGAA 
GTTTTCAACT TGCTGCACTC AGGTGCTAGT TATCTTCGTG TTCACCAGCA GGGTTTCGGT 
GCCGTCGGTG TGTCCAAGAA GTACGGAAAG CGGTCCTACG CCCGTTATCC CATCTTCTGG 
GGTTTGAGGA AGGTCGGCAA CTTGCCTAAC CCTGATCCTT CGGATGTGGG AGAATGGAGT 
AAGGAAAAGG CTATTGGTAA CGCTGACGAG GTCCATGTGG ATCCCGACTA TGAGGCCGGC 
AGGGCAGACC TCAAACGCCA GGCTCAGGAA TGGGCTGGTC TTGATGTCAA CCCCGACGCT 
GATCTAATGG TGTTCGTTGG TCGTTGGTCC ATGCAGAAAG GTGTCGATTT AATCGCCGAT 
GTGATGCCAG CTGTTCTTGA AGCTCGCCCT AACGTGCAGG TAATCTGTGT TGGACCGGTT 
ATCGATCTTT ATGGTAAATT CGCCGCCCTG AAGTTGGACC ACATGATGAA GGTTTACCCT 
GGACGTGTGT TCTCGAGACC TGAATTCACC GCTCTTCCGC CTTATATCTT CTCTGGTGCT 
GAATTCGCGC TTATTCCTTC TCGTGACGAG CCCTTTGGTC TAGTCGCAGT CGAGTTCGGC 
CGTAAGGGAG CCTTAGGTAT CGGTGCCCGT GTCGGTGGTC TCGGTCAGAT GCCTGGATGG 
TGGTATAACG TCGAATCTAC TGCGACATCT CATCTTCTGT ACCAGTTCAA GCTTGCCATC 
GACGCCGCAC TCAACTCGAA ACAAGAGACC AGAGCCATGA TGCGTGCCCG TTCTGCTAAA 
CAGCGATTCC CCGTCGCCCA ATGGGTCGAG GACTTGGAAA TCCTGCAAAC CACCGCAATC 
CAAGTACACA ACAAGGAATT GGTCAAGCAC AACGGTCGTC CGTTCACTCC GACTGGAACG 
ACTACTCCTA GTGGCCTTAT GACTCAACCT GCGAGCCCCC TCGGGACCCC AGGAATGCAA 
ACTCCTCTTG CTCATTCTAG GGAAAGCAGC TACTCGAACC TCAACCGTCT AAGTGAATAC 
GTTACCCAGC CAAAGACCAG CTACAGCAGA GATCCCAGCC CTAGCGGCAC GGAGAAGCCG 
AAATCAGGAC TTCAGCGACA GCTTTCCCTT GGTGTTCGCT CTGGACCTGG TCATCAGAGC 
CGTCGTGGTC GCGCTCGCCA GCGTGACAGC ATCCCAGAAC ACGAAGACAC CCAGGAAGCT 
CACGGTGGCG CCATTACTGA TGTTGAGGAA GAAAGCAGTG ACGACGACAT TGTCAACCAT 
TACGCGGATG ACGAGTATAC TCTTACACCT GCCCAAGTCG AAGAAGGCCG TAGGTTGCAG 
GCCGCCCAGC AACAGGCTGG TATACGCATG CCGTTGAGTC CAGGTGGTAG ACGCTACAGC 
CAAGACTCGT TGCATCCGAG AAATGTCCAG CCTCCTTCGA GTCCCGGAAC ACCCCCAGCC 
GCTTCCCAGA GTCTCCTTCC TCCCCCTAGG CTCCTCGATC CCGGCAGTCG TCTCATCTAT 
CGTGATAAGC ACGGCGCTTC CCCTATCGGC TCGTTCTACG ATGATAACGG CTCCCGTATG 
AGTGGTAGCG ATGGCCCTCA CTCCAATGAC AGTGAAGACG ACGAGTTCCT CCTCGGAAAG 
GACTATGTCC CTCCCACCGG TCTCAAGAAG TGGATGCAGA TTCGCATCGG TGACTGGCCT 
ATCTACTCCT TGTTCCTCGC TTTAGGCCAA ATCATTGCTG CCAACTCGTA CCAGATCACA 
TTGCTCACGG GCGAAGTCGG TCAAACTGCC GAGAAACTGT ACGGAATTGC AACCACGTAT 
TTGATCACGT CTATTCTCTG GTGGCTTGTG TTCCGCTACT TCAAATCCGT CGTCTGTCTG 
TCTGCGCCAT GGTTCTTGTA CGGTATCGCC TTCATCTTCA TTGGATCCGC CCATTTTGAG 
AGCAACTCTT TCACTCGGGG ATGGATTCAA AATGTCGGTA GTGGGTTCTA CGCCGCGGCC 
TCGTCTAGTG GTTCTTTCTT CTTCGCGCTA AACTTCGGTG ATGAAGGTGG TGCACCTGTG 
GAAACATGGA TCTTCCGTGC ATGTCTCATT CAGGGTATCC AGTCCGCCTA TGTTATTGCT 
CTCTGGTACT GGGGTTCAAC CCTGTCGCAG GCACAAAGTG AGGGTCTCTT GACTCCTACA 
AACAATATCT CCAATTCTTG GAAGATGAGT GCCATCTGTT ACCCCATTGC CGCCGCCCTT 
TTCGGAATTG GTTTGCTCTT GACATTCGGC CTGCCCAACT ATTACCGTCA AACCCCTGGC 
AAGGTCGCTT CCTTCTACAA ATCCGTGTTC CGTCGTAAGA TCGTCCTCTG GAACTTTGTC 
GCGGTCATCC TTCAGAACTT CTTCCTCAGC GCCCCCTACG GCCGCAACTG GCAGTTCCTC 
TGGACATCCC ACCACGCACA TCACTGGCAA ATCGTCATCC TCTGTGTTGT TTTCTACGGC 
TTCGTATGGG CAGGTTTCCT ATTCGTCGTC AGTCGCTACT TCAAATCACA CAGCTGGTTC 
CTCCCCGTGT TCGCCTGTGG CCTCGGAGCT CCCCGCTGGG CACAAATCTG GTGGGGTGTG 
TCTGGCATTG GCTACTACCT CCCTTGGGTG ACAGGAGGAT ATACCGGCGG CGCGCTCGTC 
TCCCGAAGTG TCTGGCTCTG GCTCGGCGTG CTGGACTCGA TCCAGGGTCT CGGCTTCGGT 
ATCATCCTCT TGCAAACCCT GACTCGCATG CACATGCTTT TCTGTCTTGT TTGTTCTCAA 
GTCCTTGGTT CTATCGCTAC GATCTGCGCG AGAGCCTTCG CCCCTAATAA TGTGGGCCCA 
GGGCCGGTTT CGCCTGATCC TACCTTTGGT GGGAGTGCGG TTGCGAATGC CTGGTTCTGG 
GTTGCTCTGT TTTGTCAGTT GTTGGTCTGT GCCGGTTACA TCCTCTTCTT CCGGAAAGAA 
CAGCTGTCAA AGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome Sequence of Aspergillus flavus NRRL 3357, a Strain That Causes Aflatoxin Contamination of Food and Feed.
Genome announcements3(2)(2015 Apr)
Nierman WC,Yu J,Fedorova-Abrams ND,Losada L,Cleveland TE,Bhatnagar D,Bennett JW,Dean R,Payne GA

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AFLA_023460 cDNA ORF clone, Aspergillus flavus NRRL3357

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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