LOC101168576 cDNA ORF clone, Oryzias latipes(Japanese medaka)

The following LOC101168576 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101168576 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OJa111306 XM_020711737.2
Latest version!
Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OJa111307 XM_011487658.3
Latest version!
Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OJa111308 XM_020711738.2
Latest version!
Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OJa111309 XM_020711740.2
Latest version!
Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OJa31759 XM_011487658.2
Latest version!
Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OJa74010 XM_020711737.1
Latest version!
Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OJa74011 XM_020711738.1
Latest version!
Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OJa74012 XM_020711739.2
Latest version!
Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OJa74013 XM_020711740.1
Latest version!
Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
View Old Accession Versions >>
OJa74012 XM_020711739.1 Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OJa111306
    Clone ID Related Accession (Same CDS sequence) XM_020711737.2
    Accession Version XM_020711737.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5133bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1518537600000
    Organism Oryzias latipes(Japanese medaka)
    Product collagen alpha-6(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_020711737.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGTGACGTG GGTTTGCCCG GTGAGCCCGG CCAGCCTCTC
    ATCAATGGGA TTGTGGAGTT TGTGGGTTAT CCCAAAGGAG ACAAAGGAGA GCAGGGGGAG
    CCAGGTCCAA AAGGATCAAT TGGATTTCAA GGATTACCTG GACTGCCTGG AGCCTTTGGT
    TATCCAGGTG AAATTGGAGA GAAAGGCATC CCTGGATATC CTGGAGGAAG AGGTTCTCCA
    GGTTTTAATG GACCTCAGGG TGCTCCAGGA TTACCGGGAC AGAGAGGAGA CACAGGTTAC
    TCCGGCAGGC CAGGTTACGA CGGGCCAAAG GGCGACCCAG GAGACCGAGG TCCTCGAGGT
    CCTCCTATTT ATGTCAGTAA TATTGGCAGA ACTGCTCAAG GTCCTTCAGG AGATCCTGGT
    TACAGCGGTG CTCCTGGCGC TCCCGGAGAC CCAGGACCCA CAGGTTTCCC AGGACTTCCA
    GGTGAACCTG GACGTCTTCT GCCTGGTTCA GAAACATCAG GACTTCCAGG TCTACCCGGA
    CTACCTGGCC CGGAAGGAGA GAAGGGAAAC CCAGGTCCAC TGGTTAATGG TCCTGAAGGA
    TTCCCAGGTC TGCCTGGACC CCCTGGAGGT CCTGGCCCCC CGGGTCCTCC TGGCAGGACC
    ACTGATCCCT GTGTCATCTA TCCTGTCAGT CCTGAAGGCT CTCCTGAGGA TGTGACCTCC
    GGTCTACCCG GAGTACCCGG GCAGCCTGGC CCCAGAGGAC CTCCTGGAGA CCGAGGTTTC
    AAAGGCTTCG GAGGTGACCC TGGTGACTGT GACTGTGAGG GAGGGGGCTC CTCAGGGGCA
    ACTGGGTTAC CTGGACCTCC TGGACTGAAT GGCAGCCCTG GTGCCACTGG AAGGAAGGGT
    GAACAGGGTG ACACTGGATC ATCGGGGTTC AGCGGGCCTT CTGGTTCTGA TGGGCGGCCT
    GGTAGCAGGG GCTTCTTCGG TTTGAAAGGA GAAAAGGGGG AACCATATTT ATCACGGGGC
    TCTTCGAGCA AGGGGGATCG TGGAGCACCC GGTCCCAGAG GACCCATAGG TGAAACTGGA
    AGACCTGGCA GAGATGGGCT GCCTGGATTT GCTGGACTTC CTGGAAGTCC TGGCGACCCT
    GGCTTTGGTA CAGTTGGAGA AAAGGGATTC TTAGGGTTCC CGGGAATCAA AGGTCGTCCT
    GGAGCCCCTG GAGAGTCCGG CATTGGCTAC ATTGGACTGC CTGGCTTGCG AGGAGAGCCT
    GGAGACCCAG GTTTTTCTGG GTTGCCTGGG CCACCAGGTT TACCTGGACC AAGAGGTGAA
    AGCGCCTGTG GACGGGTTAA TGGCGCCGAA ACGGGAACGG GTGATTTGTT GCCCTGCAAA
    ATACCTGGAC AAGTCGGAGA CCCTGGGATT CCTGGCTTAC CTGGACGCCC TGGCCTTAAG
    GGTCAGCCGG GTCGGCCGGG AGAACCTGGA CGTCCAGGAC TTGATGGTTC CAAAGGGGAG
    AGGGGAGATC CTGGCTTTGG GAACCAGCCC GGTTCTCCAG GTTTTCCAGG ACCTAGAGGG
    GACCCAGGAA TCCCAGGTGT TCCTGGGCTG CGCATTGATG GGACCAGGGG AAGAAATGGT
    GTTCCAGGGC TTCCTGGAGC CAAAGGACAG CCTGGAGAGA TTCTTGGAGC CATACCTGGA
    CTTCCAGGAC GAGATGGTGA ACCTGGAATA CGTGGGGACA AGGGCCAGCC CGGCTCTCCA
    GGAGTTCCTG GAGGTCCTGG TCTGGATGGA AAGAACGGTA TTCCTGGACT GAGAGGAGAA
    CGTGGAATTG ATGGTCTGCC GGGTGTTCCT GGTTTTCCTG GACCTCCGGG TGAGGTTATC
    GGCCCTACTC TTGGACTGCC TGGACCTCCT GGCAGCAAAG GACAGATTGG ATCACCGGGC
    TGCCCAGGTC TTCCTGGGGA GAAAGGAGAC ACAGGGGATC CAGGTTTCCC AGGACCTGCA
    GGAATGAAAG GAACACCTGG GTTTCCTGGT ACTCCTGGAA CACCAGGTCC ACGGGGACCG
    CCTGGACCTC CAGGACCTGG AACTGTTGAC GGATTACCTG GAATTCCTGG AGTAAAAGGG
    GAAAGAGGTT TTGTTGGACT CACTGGTTTT CCTGGACAGT CTGGACGGCC CGGAACACCA
    GGATTTCCAG GAGGGAAAGG TCTGCCGGGG GAGCCTGGAA GAAATGGATC ACCTGGCAGA
    CTTGGACCTC AAGGCAACAG AGGTGACAGA GGGCTTCCAG GTCTTCCAGG CCCCCCCAGT
    ATTTACACAC CAGTTGTGGC GAAGGGAGAA CTGGGAGATC CTGGAGCCAG AGGGTTTGAT
    GGTTTTCCTG GACCCAGAGG TGACAAGGGT CTGCCTGGCC TGCCCGGTCG ACAAGGTCGC
    CCTGGTCTTC CTGGTCTACC TGAGCAAAGT AAAGGGCAAC CCGGGCAGCC GGGCCTACCT
    GGACTGCCGG GATCTCCAGG CTTACCGGGA CCAAAGGGAG AAGTTGGAAT CAATGGGTTC
    CCTGGCATGC CAGGACCAAG AGGAGATGAT GGAATACCTG GTGCACCCGG AGGTCCTGGA
    GAACCAGGTC GTCCAGGTGG AAAAGGTCAA CCTGGGGAAA CCTTTGGTTT TCCTGGCTCA
    CCTGGACAGA AAGGTCAACC GGGAGATCCA GGCAGCCCAG GACGTCAAGG CTTTGATGGA
    GCCCCCGGTG ATAATGGCTT GTCTGGAAAC CCTGGTTTCC CTGGAATAAA AGGTGGTCCT
    GGTGAACCAG GACGGCCTGG ATTACCTGGA TCTGTTGGTT TTCCAGGCCC TAAAGGGCTG
    TCAACATTCC CTGGAAGAAA AGGAGCAGAT GGGCCCCCAG GACAGCCTGG ATTTCCAGGA
    GGTCCAGGAG CCAGGGGTGT GCCTGGACCT CCTGGCCTGG ATGGACTCAA TGGCCTCGCT
    GGATCCAAAG GCCTTCCTGG AACCCCAGGT ATTACTTTAG GAGGTCGCCC TGGTGCTCCT
    GGTATCCCAG GATTGAAGGG AGAGAGGGGA GATTCTTATC CTGGACAACG AGGCTTTGAT
    GGAGTAAAAG GACAAAAAGG AGAGCCTGGT CTTCCTGGAA CTTCAGGAAT TCCTGGTCAA
    CCTGGACTTC CTGGAGAGTC AGCGTTAGCA GGCATTGCTG GACCTGTGGG GGACCCAGGA
    CTTCCTGGAT TGGATGGAAA CTATGGTTTT CAAGGTCCAC CTGGTCCTCC TGGTCCCCCG
    GGTTCAGGAA CAATACAAGG AGAAAGAGGT GAACCAGGGC TGCCAGGCTT CCCGGGATCT
    CCTGGAAGAA AGGGAGAACC CGGTTTCAAT GGAGGCCCTG GATACCCAGG TAGCGCCGGT
    GTCAAAGGAT CTCCTGGTGA AAGTGGTTTT AATGGACGTC CAGGTCTTAA AGGTGTGCCT
    GGAGATCCTG GCTTCTATGG AATCAAGGGA TCTAAAGGAA CACCAGGCCA AAAAGGACGG
    AAGGGTCCTC CTGGAGTTGT GCTGCCTTCC CCTATTGTGG AATCACAGCC TTCTGGCGAC
    ATGGGTCTTC CTGGTGAAAG GGGACCCTTG GGTTACCGTG GAGACTCTGG TTGGAGTGGC
    ATTCCAGGGC GTCCTGGTCC AAAAGGACGA CCCGGAGCCC CAGGTAACCT TGGAAGGACT
    GGACCGTCTG GTCTGCCTGG TCCGTTAGGT GATCCCGGCT CCCCTGGTTT ACCTGGAACA
    AGTGGAAGAC AAGGCCCCCC TGGAAACATT GGCCTCCCTG GCATTCCTGG CAGCTCCAGC
    CGCAGTATCA GCATTGGCTA CACTCTGGTA AAGCACAGCC AGAGCGAGCA GGTCCCCATG
    TGTCCTCAGG GCATGCCTTC ATTGTGGAAT GGGTACAGCT TGCTGTATGT GGAGGGACAG
    GAAAAAGCAC ACAACCAAGA CCTGGGTCAG CCTGGCTCGT GCCTCCCCAG GTTCAGCACC
    ATGCCCTTCC TGTACTGCTC CCCCAATGAG GTTTGCTTCT TTGCTAGTCG CAATGACAAA
    TCCTACTGGC TCTCCACATC TGCACCCATT CCCATGATGC CCGTGTCTGC GGGGCAGATC
    CAGCCTTACA TCAGCAGATG TTCAGTATGT GAGGCACCAT CTCAGGCTGT GGCGGTCCAC
    AGTCAGGACA TGACCATCCC CATGTGCCCC CCTGGGTGGA GAAGTCTTTG GATTGGATAT
    TCCTTCTTGA TGCACACGGC GGCTGGTGCA GAAGGCGGCG GTCAGTCCCT GATGTCTCCC
    GGCTCCTGCT TGGAGGATTT CCGCGCCACT CCATTCATTG AGTGCAATGG AGCCAAAGGC
    ACATGCCACT ACTTTGCCAA CAAATACAGC TTCTGGCTGA CCACCGTGGA TCCCAAGCGG
    GAGTTTGTGT CTTCACCAAA CCAGGAGACC CTGAAGGCGG GCCAGGAGCG CTCCAGAGTC
    AGTCGCTGTC AGGTCTGCAG CAAACTGCTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020567396.2
    CDS480..5612
    Translation

    Target ORF information:

    RefSeq Version XM_020711737.2
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_020711737.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGTGACGTG GGTTTGCCCG GTGAGCCCGG CCAGCCTCTC
    ATCAATGGGA TTGTGGAGTT TGTGGGTTAT CCCAAAGGAG ACAAAGGAGA GCAGGGGGAG
    CCAGGTCCAA AAGGATCAAT TGGATTTCAA GGATTACCTG GACTGCCTGG AGCCTTTGGT
    TATCCAGGTG AAATTGGAGA GAAAGGCATC CCTGGATATC CTGGAGGAAG AGGTTCTCCA
    GGTTTTAATG GACCTCAGGG TGCTCCAGGA TTACCGGGAC AGAGAGGAGA CACAGGTTAC
    TCCGGCAGGC CAGGTTACGA CGGGCCAAAG GGCGACCCAG GAGACCGAGG TCCTCGAGGT
    CCTCCTATTT ATGTCAGTAA TATTGGCAGA ACTGCTCAAG GTCCTTCAGG AGATCCTGGT
    TACAGCGGTG CTCCTGGCGC TCCCGGAGAC CCAGGACCCA CAGGTTTCCC AGGACTTCCA
    GGTGAACCTG GACGTCTTCT GCCTGGTTCA GAAACATCAG GACTTCCAGG TCTACCCGGA
    CTACCTGGCC CGGAAGGAGA GAAGGGAAAC CCAGGTCCAC TGGTTAATGG TCCTGAAGGA
    TTCCCAGGTC TGCCTGGACC CCCTGGAGGT CCTGGCCCCC CGGGTCCTCC TGGCAGGACC
    ACTGATCCCT GTGTCATCTA TCCTGTCAGT CCTGAAGGCT CTCCTGAGGA TGTGACCTCC
    GGTCTACCCG GAGTACCCGG GCAGCCTGGC CCCAGAGGAC CTCCTGGAGA CCGAGGTTTC
    AAAGGCTTCG GAGGTGACCC TGGTGACTGT GACTGTGAGG GAGGGGGCTC CTCAGGGGCA
    ACTGGGTTAC CTGGACCTCC TGGACTGAAT GGCAGCCCTG GTGCCACTGG AAGGAAGGGT
    GAACAGGGTG ACACTGGATC ATCGGGGTTC AGCGGGCCTT CTGGTTCTGA TGGGCGGCCT
    GGTAGCAGGG GCTTCTTCGG TTTGAAAGGA GAAAAGGGGG AACCATATTT ATCACGGGGC
    TCTTCGAGCA AGGGGGATCG TGGAGCACCC GGTCCCAGAG GACCCATAGG TGAAACTGGA
    AGACCTGGCA GAGATGGGCT GCCTGGATTT GCTGGACTTC CTGGAAGTCC TGGCGACCCT
    GGCTTTGGTA CAGTTGGAGA AAAGGGATTC TTAGGGTTCC CGGGAATCAA AGGTCGTCCT
    GGAGCCCCTG GAGAGTCCGG CATTGGCTAC ATTGGACTGC CTGGCTTGCG AGGAGAGCCT
    GGAGACCCAG GTTTTTCTGG GTTGCCTGGG CCACCAGGTT TACCTGGACC AAGAGGTGAA
    AGCGCCTGTG GACGGGTTAA TGGCGCCGAA ACGGGAACGG GTGATTTGTT GCCCTGCAAA
    ATACCTGGAC AAGTCGGAGA CCCTGGGATT CCTGGCTTAC CTGGACGCCC TGGCCTTAAG
    GGTCAGCCGG GTCGGCCGGG AGAACCTGGA CGTCCAGGAC TTGATGGTTC CAAAGGGGAG
    AGGGGAGATC CTGGCTTTGG GAACCAGCCC GGTTCTCCAG GTTTTCCAGG ACCTAGAGGG
    GACCCAGGAA TCCCAGGTGT TCCTGGGCTG CGCATTGATG GGACCAGGGG AAGAAATGGT
    GTTCCAGGGC TTCCTGGAGC CAAAGGACAG CCTGGAGAGA TTCTTGGAGC CATACCTGGA
    CTTCCAGGAC GAGATGGTGA ACCTGGAATA CGTGGGGACA AGGGCCAGCC CGGCTCTCCA
    GGAGTTCCTG GAGGTCCTGG TCTGGATGGA AAGAACGGTA TTCCTGGACT GAGAGGAGAA
    CGTGGAATTG ATGGTCTGCC GGGTGTTCCT GGTTTTCCTG GACCTCCGGG TGAGGTTATC
    GGCCCTACTC TTGGACTGCC TGGACCTCCT GGCAGCAAAG GACAGATTGG ATCACCGGGC
    TGCCCAGGTC TTCCTGGGGA GAAAGGAGAC ACAGGGGATC CAGGTTTCCC AGGACCTGCA
    GGAATGAAAG GAACACCTGG GTTTCCTGGT ACTCCTGGAA CACCAGGTCC ACGGGGACCG
    CCTGGACCTC CAGGACCTGG AACTGTTGAC GGATTACCTG GAATTCCTGG AGTAAAAGGG
    GAAAGAGGTT TTGTTGGACT CACTGGTTTT CCTGGACAGT CTGGACGGCC CGGAACACCA
    GGATTTCCAG GAGGGAAAGG TCTGCCGGGG GAGCCTGGAA GAAATGGATC ACCTGGCAGA
    CTTGGACCTC AAGGCAACAG AGGTGACAGA GGGCTTCCAG GTCTTCCAGG CCCCCCCAGT
    ATTTACACAC CAGTTGTGGC GAAGGGAGAA CTGGGAGATC CTGGAGCCAG AGGGTTTGAT
    GGTTTTCCTG GACCCAGAGG TGACAAGGGT CTGCCTGGCC TGCCCGGTCG ACAAGGTCGC
    CCTGGTCTTC CTGGTCTACC TGAGCAAAGT AAAGGGCAAC CCGGGCAGCC GGGCCTACCT
    GGACTGCCGG GATCTCCAGG CTTACCGGGA CCAAAGGGAG AAGTTGGAAT CAATGGGTTC
    CCTGGCATGC CAGGACCAAG AGGAGATGAT GGAATACCTG GTGCACCCGG AGGTCCTGGA
    GAACCAGGTC GTCCAGGTGG AAAAGGTCAA CCTGGGGAAA CCTTTGGTTT TCCTGGCTCA
    CCTGGACAGA AAGGTCAACC GGGAGATCCA GGCAGCCCAG GACGTCAAGG CTTTGATGGA
    GCCCCCGGTG ATAATGGCTT GTCTGGAAAC CCTGGTTTCC CTGGAATAAA AGGTGGTCCT
    GGTGAACCAG GACGGCCTGG ATTACCTGGA TCTGTTGGTT TTCCAGGCCC TAAAGGGCTG
    TCAACATTCC CTGGAAGAAA AGGAGCAGAT GGGCCCCCAG GACAGCCTGG ATTTCCAGGA
    GGTCCAGGAG CCAGGGGTGT GCCTGGACCT CCTGGCCTGG ATGGACTCAA TGGCCTCGCT
    GGATCCAAAG GCCTTCCTGG AACCCCAGGT ATTACTTTAG GAGGTCGCCC TGGTGCTCCT
    GGTATCCCAG GATTGAAGGG AGAGAGGGGA GATTCTTATC CTGGACAACG AGGCTTTGAT
    GGAGTAAAAG GACAAAAAGG AGAGCCTGGT CTTCCTGGAA CTTCAGGAAT TCCTGGTCAA
    CCTGGACTTC CTGGAGAGTC AGCGTTAGCA GGCATTGCTG GACCTGTGGG GGACCCAGGA
    CTTCCTGGAT TGGATGGAAA CTATGGTTTT CAAGGTCCAC CTGGTCCTCC TGGTCCCCCG
    GGTTCAGGAA CAATACAAGG AGAAAGAGGT GAACCAGGGC TGCCAGGCTT CCCGGGATCT
    CCTGGAAGAA AGGGAGAACC CGGTTTCAAT GGAGGCCCTG GATACCCAGG TAGCGCCGGT
    GTCAAAGGAT CTCCTGGTGA AAGTGGTTTT AATGGACGTC CAGGTCTTAA AGGTGTGCCT
    GGAGATCCTG GCTTCTATGG AATCAAGGGA TCTAAAGGAA CACCAGGCCA AAAAGGACGG
    AAGGGTCCTC CTGGAGTTGT GCTGCCTTCC CCTATTGTGG AATCACAGCC TTCTGGCGAC
    ATGGGTCTTC CTGGTGAAAG GGGACCCTTG GGTTACCGTG GAGACTCTGG TTGGAGTGGC
    ATTCCAGGGC GTCCTGGTCC AAAAGGACGA CCCGGAGCCC CAGGTAACCT TGGAAGGACT
    GGACCGTCTG GTCTGCCTGG TCCGTTAGGT GATCCCGGCT CCCCTGGTTT ACCTGGAACA
    AGTGGAAGAC AAGGCCCCCC TGGAAACATT GGCCTCCCTG GCATTCCTGG CAGCTCCAGC
    CGCAGTATCA GCATTGGCTA CACTCTGGTA AAGCACAGCC AGAGCGAGCA GGTCCCCATG
    TGTCCTCAGG GCATGCCTTC ATTGTGGAAT GGGTACAGCT TGCTGTATGT GGAGGGACAG
    GAAAAAGCAC ACAACCAAGA CCTGGGTCAG CCTGGCTCGT GCCTCCCCAG GTTCAGCACC
    ATGCCCTTCC TGTACTGCTC CCCCAATGAG GTTTGCTTCT TTGCTAGTCG CAATGACAAA
    TCCTACTGGC TCTCCACATC TGCACCCATT CCCATGATGC CCGTGTCTGC GGGGCAGATC
    CAGCCTTACA TCAGCAGATG TTCAGTATGT GAGGCACCAT CTCAGGCTGT GGCGGTCCAC
    AGTCAGGACA TGACCATCCC CATGTGCCCC CCTGGGTGGA GAAGTCTTTG GATTGGATAT
    TCCTTCTTGA TGCACACGGC GGCTGGTGCA GAAGGCGGCG GTCAGTCCCT GATGTCTCCC
    GGCTCCTGCT TGGAGGATTT CCGCGCCACT CCATTCATTG AGTGCAATGG AGCCAAAGGC
    ACATGCCACT ACTTTGCCAA CAAATACAGC TTCTGGCTGA CCACCGTGGA TCCCAAGCGG
    GAGTTTGTGT CTTCACCAAA CCAGGAGACC CTGAAGGCGG GCCAGGAGCG CTCCAGAGTC
    AGTCGCTGTC AGGTCTGCAG CAAACTGCTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa111307
    Clone ID Related Accession (Same CDS sequence) XM_011487658.3
    Accession Version XM_011487658.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1518537600000
    Organism Oryzias latipes(Japanese medaka)
    Product collagen alpha-6(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_011487658.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGCGACGTG GGTCTTCCCG GACCCAGTGG TGCTCCCGGA
    AGCGGGTCTC TAACGGCCCC AAAAATTCTC ACTATCTACA AGGGAGATAA GGGGGAGCCA
    GGTCCAAAAG GATCAATTGG ATTTCAAGGA TTACCTGGAC TGCCTGGAGC CTTTGGTTAT
    CCAGGTGAAA TTGGAGAGAA AGGCATCCCT GGATATCCTG GAGGAAGAGG TTCTCCAGGT
    TTTAATGGAC CTCAGGGTGC TCCAGGATTA CCGGGACAGA GAGGAGACAC AGGTTACTCC
    GGCAGGCCAG GTTACGACGG GCCAAAGGGC GACCCAGGAG ACCGAGGTCC TCGAGGTCCT
    CCTATTTATG TCAGTAATAT TGGCAGAACT GCTCAAGGTC CTTCAGGAGA TCCTGGTTAC
    AGCGGTGCTC CTGGCGCTCC CGGAGACCCA GGACCCACAG GTTTCCCAGG ACTTCCAGGT
    GAACCTGGAC GTCTTCTGCC TGGTTCAGAA ACATCAGGAC TTCCAGGTCT ACCCGGACTA
    CCTGGCCCGG AAGGAGAGAA GGGAAACCCA GGTCCACTGG TTAATGGTCC TGAAGGATTC
    CCAGGTCTGC CTGGACCCCC TGGAGGTCCT GGCCCCCCGG GTCCTCCTGG CAGGACCACT
    GATCCCTGTG TCATCTATCC TGTCAGTCCT GAAGGCTCTC CTGAGGATGT GACCTCCGGT
    CTACCCGGAG TACCCGGGCA GCCTGGCCCC AGAGGACCTC CTGGAGACCG AGGTTTCAAA
    GGCTTCGGAG GTGACCCTGG TGACTGTGAC TGTGAGGGAG GGGGCTCCTC AGGGGCAACT
    GGGTTACCTG GACCTCCTGG ACTGAATGGC AGCCCTGGTG CCACTGGAAG GAAGGGTGAA
    CAGGGTGACA CTGGATCATC GGGGTTCAGC GGGCCTTCTG GTTCTGATGG GCGGCCTGGT
    AGCAGGGGCT TCTTCGGTTT GAAAGGAGAA AAGGGGGAAC CATATTTATC ACGGGGCTCT
    TCGAGCAAGG GGGATCGTGG AGCACCCGGT CCCAGAGGAC CCATAGGTGA AACTGGAAGA
    CCTGGCAGAG ATGGGCTGCC TGGATTTGCT GGACTTCCTG GAAGTCCTGG CGACCCTGGC
    TTTGGTACAG TTGGAGAAAA GGGATTCTTA GGGTTCCCGG GAATCAAAGG TCGTCCTGGA
    GCCCCTGGAG AGTCCGGCAT TGGCTACATT GGACTGCCTG GCTTGCGAGG AGAGCCTGGA
    GACCCAGGTT TTTCTGGGTT GCCTGGGCCA CCAGGTTTAC CTGGACCAAG AGGTGAAAGC
    GCCTGTGGAC GGGTTAATGG CGCCGAAACG GGAACGGGTG ATTTGTTGCC CTGCAAAATA
    CCTGGACAAG TCGGAGACCC TGGGATTCCT GGCTTACCTG GACGCCCTGG CCTTAAGGGT
    CAGCCGGGTC GGCCGGGAGA ACCTGGACGT CCAGGACTTG ATGGTTCCAA AGGGGAGAGG
    GGAGATCCTG GCTTTGGGAA CCAGCCCGGT TCTCCAGGTT TTCCAGGACC TAGAGGGGAC
    CCAGGAATCC CAGGTGTTCC TGGGCTGCGC ATTGATGGGA CCAGGGGAAG AAATGGTGTT
    CCAGGGCTTC CTGGAGCCAA AGGACAGCCT GGAGAGATTC TTGGAGCCAT ACCTGGACTT
    CCAGGACGAG ATGGTGAACC TGGAATACGT GGGGACAAGG GCCAGCCCGG CTCTCCAGGA
    GTTCCTGGAG GTCCTGGTCT GGATGGAAAG AACGGTATTC CTGGACTGAG AGGAGAACGT
    GGAATTGATG GTCTGCCGGG TGTTCCTGGT TTTCCTGGAC CTCCGGGTGA GGTTATCGGC
    CCTACTCTTG GACTGCCTGG ACCTCCTGGC AGCAAAGGAC AGATTGGATC ACCGGGCTGC
    CCAGGTCTTC CTGGGGAGAA AGGAGACACA GGGGATCCAG GTTTCCCAGG ACCTGCAGGA
    ATGAAAGGAA CACCTGGGTT TCCTGGTACT CCTGGAACAC CAGGTCCACG GGGACCGCCT
    GGACCTCCAG GACCTGGAAC TGTTGACGGA TTACCTGGAA TTCCTGGAGT AAAAGGGGAA
    AGAGGTTTTG TTGGACTCAC TGGTTTTCCT GGACAGTCTG GACGGCCCGG AACACCAGGA
    TTTCCAGGAG GGAAAGGTCT GCCGGGGGAG CCTGGAAGAA ATGGATCACC TGGCAGACTT
    GGACCTCAAG GCAACAGAGG TGACAGAGGG CTTCCAGGTC TTCCAGGCCC CCCCAGTATT
    TACACACCAG TTGTGGCGAA GGGAGAACTG GGAGATCCTG GAGCCAGAGG GTTTGATGGT
    TTTCCTGGAC CCAGAGGTGA CAAGGGTCTG CCTGGCCTGC CCGGTCGACA AGGTCGCCCT
    GGTCTTCCTG GTCTACCTGA GCAAAGTAAA GGGCAACCCG GGCAGCCGGG CCTACCTGGA
    CTGCCGGGAT CTCCAGGCTT ACCGGGACCA AAGGGAGAAG TTGGAATCAA TGGGTTCCCT
    GGCATGCCAG GACCAAGAGG AGATGATGGA ATACCTGGTG CACCCGGAGG TCCTGGAGAA
    CCAGGTCGTC CAGGTGGAAA AGGTCAACCT GGGGAAACCT TTGGTTTTCC TGGCTCACCT
    GGACAGAAAG GTCAACCGGG AGATCCAGGC AGCCCAGGAC GTCAAGGCTT TGATGGAGCC
    CCCGGTGATA ATGGCTTGTC TGGAAACCCT GGTTTCCCTG GAATAAAAGG TGGTCCTGGT
    GAACCAGGAC GGCCTGGATT ACCTGGATCT GTTGGTTTTC CAGGCCCTAA AGGGCTGTCA
    ACATTCCCTG GAAGAAAAGG AGCAGATGGG CCCCCAGGAC AGCCTGGATT TCCAGGAGGT
    CCAGGAGCCA GGGGTGTGCC TGGACCTCCT GGCCTGGATG GACTCAATGG CCTCGCTGGA
    TCCAAAGGCC TTCCTGGAAC CCCAGGTATT ACTTTAGGAG GTCGCCCTGG TGCTCCTGGT
    ATCCCAGGAT TGAAGGGAGA GAGGGGAGAT TCTTATCCTG GACAACGAGG CTTTGATGGA
    GTAAAAGGAC AAAAAGGAGA GCCTGGTCTT CCTGGAACTT CAGGAATTCC TGGTCAACCT
    GGACTTCCTG GAGAGTCAGC GTTAGCAGGC ATTGCTGGAC CTGTGGGGGA CCCAGGACTT
    CCTGGATTGG ATGGAAACTA TGGTTTTCAA GGTCCACCTG GTCCTCCTGG TCCCCCGGGT
    TCAGGAACAA TACAAGGAGA AAGAGGTGAA CCAGGGCTGC CAGGCTTCCC GGGATCTCCT
    GGAAGAAAGG GAGAACCCGG TTTCAATGGA GGCCCTGGAT ACCCAGGTAG CGCCGGTGTC
    AAAGGATCTC CTGGTGAAAG TGGTTTTAAT GGACGTCCAG GTCTTAAAGG TGTGCCTGGA
    GATCCTGGCT TCTATGGAAT CAAGGGATCT AAAGGAACAC CAGGCCAAAA AGGACGGAAG
    GGTCCTCCTG GAGTTGTGCT GCCTTCCCCT ATTGTGGAAT CACAGCCTTC TGGCGACATG
    GGTCTTCCTG GTGAAAGGGG ACCCTTGGGT TACCGTGGAG ACTCTGGTTG GAGTGGCATT
    CCAGGGCGTC CTGGTCCAAA AGGACGACCC GGAGCCCCAG GTAACCTTGG AAGGACTGGA
    CCGTCTGGTC TGCCTGGTCC GTTAGGTGAT CCCGGCTCCC CTGGTTTACC TGGAACAAGT
    GGAAGACAAG GCCCCCCTGG AAACATTGGC CTCCCTGGCA TTCCTGGCAG CTCCAGCCGC
    AGTATCAGCA TTGGCTACAC TCTGGTAAAG CACAGCCAGA GCGAGCAGGT CCCCATGTGT
    CCTCAGGGCA TGCCTTCATT GTGGAATGGG TACAGCTTGC TGTATGTGGA GGGACAGGAA
    AAAGCACACA ACCAAGACCT GGGTCAGCCT GGCTCGTGCC TCCCCAGGTT CAGCACCATG
    CCCTTCCTGT ACTGCTCCCC CAATGAGGTT TGCTTCTTTG CTAGTCGCAA TGACAAATCC
    TACTGGCTCT CCACATCTGC ACCCATTCCC ATGATGCCCG TGTCTGCGGG GCAGATCCAG
    CCTTACATCA GCAGATGTTC AGTATGTGAG GCACCATCTC AGGCTGTGGC GGTCCACAGT
    CAGGACATGA CCATCCCCAT GTGCCCCCCT GGGTGGAGAA GTCTTTGGAT TGGATATTCC
    TTCTTGATGC ACACGGCGGC TGGTGCAGAA GGCGGCGGTC AGTCCCTGAT GTCTCCCGGC
    TCCTGCTTGG AGGATTTCCG CGCCACTCCA TTCATTGAGT GCAATGGAGC CAAAGGCACA
    TGCCACTACT TTGCCAACAA ATACAGCTTC TGGCTGACCA CCGTGGATCC CAAGCGGGAG
    TTTGTGTCTT CACCAAACCA GGAGACCCTG AAGGCGGGCC AGGAGCGCTC CAGAGTCAGT
    CGCTGTCAGG TCTGCAGCAA ACTGCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011485960.2
    CDS480..5609
    Translation

    Target ORF information:

    RefSeq Version XM_011487658.3
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011487658.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGCGACGTG GGTCTTCCCG GACCCAGTGG TGCTCCCGGA
    AGCGGGTCTC TAACGGCCCC AAAAATTCTC ACTATCTACA AGGGAGATAA GGGGGAGCCA
    GGTCCAAAAG GATCAATTGG ATTTCAAGGA TTACCTGGAC TGCCTGGAGC CTTTGGTTAT
    CCAGGTGAAA TTGGAGAGAA AGGCATCCCT GGATATCCTG GAGGAAGAGG TTCTCCAGGT
    TTTAATGGAC CTCAGGGTGC TCCAGGATTA CCGGGACAGA GAGGAGACAC AGGTTACTCC
    GGCAGGCCAG GTTACGACGG GCCAAAGGGC GACCCAGGAG ACCGAGGTCC TCGAGGTCCT
    CCTATTTATG TCAGTAATAT TGGCAGAACT GCTCAAGGTC CTTCAGGAGA TCCTGGTTAC
    AGCGGTGCTC CTGGCGCTCC CGGAGACCCA GGACCCACAG GTTTCCCAGG ACTTCCAGGT
    GAACCTGGAC GTCTTCTGCC TGGTTCAGAA ACATCAGGAC TTCCAGGTCT ACCCGGACTA
    CCTGGCCCGG AAGGAGAGAA GGGAAACCCA GGTCCACTGG TTAATGGTCC TGAAGGATTC
    CCAGGTCTGC CTGGACCCCC TGGAGGTCCT GGCCCCCCGG GTCCTCCTGG CAGGACCACT
    GATCCCTGTG TCATCTATCC TGTCAGTCCT GAAGGCTCTC CTGAGGATGT GACCTCCGGT
    CTACCCGGAG TACCCGGGCA GCCTGGCCCC AGAGGACCTC CTGGAGACCG AGGTTTCAAA
    GGCTTCGGAG GTGACCCTGG TGACTGTGAC TGTGAGGGAG GGGGCTCCTC AGGGGCAACT
    GGGTTACCTG GACCTCCTGG ACTGAATGGC AGCCCTGGTG CCACTGGAAG GAAGGGTGAA
    CAGGGTGACA CTGGATCATC GGGGTTCAGC GGGCCTTCTG GTTCTGATGG GCGGCCTGGT
    AGCAGGGGCT TCTTCGGTTT GAAAGGAGAA AAGGGGGAAC CATATTTATC ACGGGGCTCT
    TCGAGCAAGG GGGATCGTGG AGCACCCGGT CCCAGAGGAC CCATAGGTGA AACTGGAAGA
    CCTGGCAGAG ATGGGCTGCC TGGATTTGCT GGACTTCCTG GAAGTCCTGG CGACCCTGGC
    TTTGGTACAG TTGGAGAAAA GGGATTCTTA GGGTTCCCGG GAATCAAAGG TCGTCCTGGA
    GCCCCTGGAG AGTCCGGCAT TGGCTACATT GGACTGCCTG GCTTGCGAGG AGAGCCTGGA
    GACCCAGGTT TTTCTGGGTT GCCTGGGCCA CCAGGTTTAC CTGGACCAAG AGGTGAAAGC
    GCCTGTGGAC GGGTTAATGG CGCCGAAACG GGAACGGGTG ATTTGTTGCC CTGCAAAATA
    CCTGGACAAG TCGGAGACCC TGGGATTCCT GGCTTACCTG GACGCCCTGG CCTTAAGGGT
    CAGCCGGGTC GGCCGGGAGA ACCTGGACGT CCAGGACTTG ATGGTTCCAA AGGGGAGAGG
    GGAGATCCTG GCTTTGGGAA CCAGCCCGGT TCTCCAGGTT TTCCAGGACC TAGAGGGGAC
    CCAGGAATCC CAGGTGTTCC TGGGCTGCGC ATTGATGGGA CCAGGGGAAG AAATGGTGTT
    CCAGGGCTTC CTGGAGCCAA AGGACAGCCT GGAGAGATTC TTGGAGCCAT ACCTGGACTT
    CCAGGACGAG ATGGTGAACC TGGAATACGT GGGGACAAGG GCCAGCCCGG CTCTCCAGGA
    GTTCCTGGAG GTCCTGGTCT GGATGGAAAG AACGGTATTC CTGGACTGAG AGGAGAACGT
    GGAATTGATG GTCTGCCGGG TGTTCCTGGT TTTCCTGGAC CTCCGGGTGA GGTTATCGGC
    CCTACTCTTG GACTGCCTGG ACCTCCTGGC AGCAAAGGAC AGATTGGATC ACCGGGCTGC
    CCAGGTCTTC CTGGGGAGAA AGGAGACACA GGGGATCCAG GTTTCCCAGG ACCTGCAGGA
    ATGAAAGGAA CACCTGGGTT TCCTGGTACT CCTGGAACAC CAGGTCCACG GGGACCGCCT
    GGACCTCCAG GACCTGGAAC TGTTGACGGA TTACCTGGAA TTCCTGGAGT AAAAGGGGAA
    AGAGGTTTTG TTGGACTCAC TGGTTTTCCT GGACAGTCTG GACGGCCCGG AACACCAGGA
    TTTCCAGGAG GGAAAGGTCT GCCGGGGGAG CCTGGAAGAA ATGGATCACC TGGCAGACTT
    GGACCTCAAG GCAACAGAGG TGACAGAGGG CTTCCAGGTC TTCCAGGCCC CCCCAGTATT
    TACACACCAG TTGTGGCGAA GGGAGAACTG GGAGATCCTG GAGCCAGAGG GTTTGATGGT
    TTTCCTGGAC CCAGAGGTGA CAAGGGTCTG CCTGGCCTGC CCGGTCGACA AGGTCGCCCT
    GGTCTTCCTG GTCTACCTGA GCAAAGTAAA GGGCAACCCG GGCAGCCGGG CCTACCTGGA
    CTGCCGGGAT CTCCAGGCTT ACCGGGACCA AAGGGAGAAG TTGGAATCAA TGGGTTCCCT
    GGCATGCCAG GACCAAGAGG AGATGATGGA ATACCTGGTG CACCCGGAGG TCCTGGAGAA
    CCAGGTCGTC CAGGTGGAAA AGGTCAACCT GGGGAAACCT TTGGTTTTCC TGGCTCACCT
    GGACAGAAAG GTCAACCGGG AGATCCAGGC AGCCCAGGAC GTCAAGGCTT TGATGGAGCC
    CCCGGTGATA ATGGCTTGTC TGGAAACCCT GGTTTCCCTG GAATAAAAGG TGGTCCTGGT
    GAACCAGGAC GGCCTGGATT ACCTGGATCT GTTGGTTTTC CAGGCCCTAA AGGGCTGTCA
    ACATTCCCTG GAAGAAAAGG AGCAGATGGG CCCCCAGGAC AGCCTGGATT TCCAGGAGGT
    CCAGGAGCCA GGGGTGTGCC TGGACCTCCT GGCCTGGATG GACTCAATGG CCTCGCTGGA
    TCCAAAGGCC TTCCTGGAAC CCCAGGTATT ACTTTAGGAG GTCGCCCTGG TGCTCCTGGT
    ATCCCAGGAT TGAAGGGAGA GAGGGGAGAT TCTTATCCTG GACAACGAGG CTTTGATGGA
    GTAAAAGGAC AAAAAGGAGA GCCTGGTCTT CCTGGAACTT CAGGAATTCC TGGTCAACCT
    GGACTTCCTG GAGAGTCAGC GTTAGCAGGC ATTGCTGGAC CTGTGGGGGA CCCAGGACTT
    CCTGGATTGG ATGGAAACTA TGGTTTTCAA GGTCCACCTG GTCCTCCTGG TCCCCCGGGT
    TCAGGAACAA TACAAGGAGA AAGAGGTGAA CCAGGGCTGC CAGGCTTCCC GGGATCTCCT
    GGAAGAAAGG GAGAACCCGG TTTCAATGGA GGCCCTGGAT ACCCAGGTAG CGCCGGTGTC
    AAAGGATCTC CTGGTGAAAG TGGTTTTAAT GGACGTCCAG GTCTTAAAGG TGTGCCTGGA
    GATCCTGGCT TCTATGGAAT CAAGGGATCT AAAGGAACAC CAGGCCAAAA AGGACGGAAG
    GGTCCTCCTG GAGTTGTGCT GCCTTCCCCT ATTGTGGAAT CACAGCCTTC TGGCGACATG
    GGTCTTCCTG GTGAAAGGGG ACCCTTGGGT TACCGTGGAG ACTCTGGTTG GAGTGGCATT
    CCAGGGCGTC CTGGTCCAAA AGGACGACCC GGAGCCCCAG GTAACCTTGG AAGGACTGGA
    CCGTCTGGTC TGCCTGGTCC GTTAGGTGAT CCCGGCTCCC CTGGTTTACC TGGAACAAGT
    GGAAGACAAG GCCCCCCTGG AAACATTGGC CTCCCTGGCA TTCCTGGCAG CTCCAGCCGC
    AGTATCAGCA TTGGCTACAC TCTGGTAAAG CACAGCCAGA GCGAGCAGGT CCCCATGTGT
    CCTCAGGGCA TGCCTTCATT GTGGAATGGG TACAGCTTGC TGTATGTGGA GGGACAGGAA
    AAAGCACACA ACCAAGACCT GGGTCAGCCT GGCTCGTGCC TCCCCAGGTT CAGCACCATG
    CCCTTCCTGT ACTGCTCCCC CAATGAGGTT TGCTTCTTTG CTAGTCGCAA TGACAAATCC
    TACTGGCTCT CCACATCTGC ACCCATTCCC ATGATGCCCG TGTCTGCGGG GCAGATCCAG
    CCTTACATCA GCAGATGTTC AGTATGTGAG GCACCATCTC AGGCTGTGGC GGTCCACAGT
    CAGGACATGA CCATCCCCAT GTGCCCCCCT GGGTGGAGAA GTCTTTGGAT TGGATATTCC
    TTCTTGATGC ACACGGCGGC TGGTGCAGAA GGCGGCGGTC AGTCCCTGAT GTCTCCCGGC
    TCCTGCTTGG AGGATTTCCG CGCCACTCCA TTCATTGAGT GCAATGGAGC CAAAGGCACA
    TGCCACTACT TTGCCAACAA ATACAGCTTC TGGCTGACCA CCGTGGATCC CAAGCGGGAG
    TTTGTGTCTT CACCAAACCA GGAGACCCTG AAGGCGGGCC AGGAGCGCTC CAGAGTCAGT
    CGCTGTCAGG TCTGCAGCAA ACTGCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa111308
    Clone ID Related Accession (Same CDS sequence) XM_020711738.2
    Accession Version XM_020711738.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5124bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1518537600000
    Organism Oryzias latipes(Japanese medaka)
    Product collagen alpha-6(IV) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_020711738.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGTGACGTG GGTTTGCCCG GTGAGCCCGG CCAGCCTCTC
    ATCAATGGGA TTGTGGAGTT TGTGGGTTAT CCCAAAGGAG ACAAAGGAGA GCAGGGGGAG
    CCAGGTCCAA AAGGATCAAT TGGATTTCAA GGATTACCTG GACTGCCTGG AGCCTTTGGT
    TATCCAGGTG AAATTGGAGA GAAAGGCATC CCTGGATATC CTGGAGGAAG AGGTTCTCCA
    GGTTTTAATG GACCTCAGGG TGCTCCAGGA TTACCGGGAC AGAGAGGAGA CACAGGTTAC
    TCCGGCAGGC CAGGTTACGA CGGGCCAAAG GGCGACCCAG GAGACCGAGG TCCTCGAGGT
    CCTCCTATTT ATGTCAGTAA TATTGGCAGA ACTGCTCAAG GTCCTTCAGG AGATCCTGGT
    TACAGCGGTG CTCCTGGCGC TCCCGGAGAC CCAGGACCCA CAGGTTTCCC AGGACTTCCA
    GGTGAACCTG GACGTCTTCT GCCTGGTTCA GAAACATCAG GACTTCCAGG TCTACCCGGA
    CTACCTGGCC CGGAAGGAGA GAAGGGAAAC CCAGGTCCAC TGGTTAATGG TCCTGAAGGA
    TTCCCAGGTC TGCCTGGACC CCCTGGAGGT CCTGGCCCCC CGGGTCCTCC TGGCAGGACC
    ACTGATCCCT GTGTCATCTA TCCTGTCAGT CCTGAAGGCT CTCCTGAGGA TGTGACCTCC
    GGTCTACCCG GAGTACCCGG GCAGCCTGGC CCCAGAGGAC CTCCTGGAGA CCGAGGTTTC
    AAAGGCTTCG GAGGTGACCC TGGTGACTGT GACTGTGAGG GAGGGGGCTC CTCAGGGGCA
    ACTGGGTTAC CTGGACCTCC TGGACTGAAT GGCAGCCCTG GTGCCACTGG AAGGAAGGGT
    GAACAGGGTG ACACTGGATC ATCGGGGTTC AGCGGGCCTT CTGGTTCTGA TGGGCGGCCT
    GGTAGCAGGG GCTTCTTCGG TTTGAAAGGA GAAAAGGGGG AACCATATTT ATCACGGGGC
    TCTTCGAGCA AGGGGGATCG TGGAGCACCC GGTCCCAGAG GACCCATAGG TGAAACTGGA
    AGACCTGGCA GAGATGGGCT GCCTGGATTT GCTGGACTTC CTGGAAGTCC TGGCGACCCT
    GGCTTTGGTA CAGTTGGAGA AAAGGGATTC TTAGGGTTCC CGGGAATCAA AGGTCGTCCT
    GGAGCCCCTG GAGAGTCCGG CATTGGCTAC ATTGGACTGC CTGGCTTGCG AGGAGAGCCT
    GGAGACCCAG GTTTTTCTGG GTTGCCTGGG CCACCAGGTT TACCTGGACC AAGAGGTGAA
    AGCGCCTGTG GACGGGTTAA TGGCGCCGAA ACGGGAACGG GTGATTTGTT GCCCTGCAAA
    ATACCTGGAC AAGTCGGAGA CCCTGGGATT CCTGGCTTAC CTGGACGCCC TGGCCTTAAG
    GGTCAGCCGG GTCGGCCGGG AGAACCTGGA CGTCCAGGAC TTGATGGTTC CAAAGGGGAG
    AGGGGAGATC CTGGCTTTGG GAACCAGCCC GGTTCTCCAG GTTTTCCAGG ACCTAGAGGG
    GACCCAGGAA TCCCAGGTGT TCCTGGGCTG CGCATTGATG GGACCAGGGG AAGAAATGGT
    GTTCCAGGGC TTCCTGGAGC CAAAGGACAG CCTGGAGAGA TTCTTGGAGC CATACCTGGA
    CTTCCAGGAC GAGATGGTGA ACCTGGAATA CGTGGGGACA AGGGCCAGCC CGGCTCTCCA
    GGAGTTCCTG GAGGTCCTGG TCTGGATGGA AAGAACGGTA TTCCTGGACT GAGAGGAGAA
    CGTGGAATTG ATGGTCTGCC GGGTGTTCCT GGTTTTCCTG GACCTCCGGG TGAGGTTATC
    GGCCCTACTC TTGGACTGCC TGGACCTCCT GGCAGCAAAG GACAGATTGG ATCACCGGGT
    CTTCCTGGGG AGAAAGGAGA CACAGGGGAT CCAGGTTTCC CAGGACCTGC AGGAATGAAA
    GGAACACCTG GGTTTCCTGG TACTCCTGGA ACACCAGGTC CACGGGGACC GCCTGGACCT
    CCAGGACCTG GAACTGTTGA CGGATTACCT GGAATTCCTG GAGTAAAAGG GGAAAGAGGT
    TTTGTTGGAC TCACTGGTTT TCCTGGACAG TCTGGACGGC CCGGAACACC AGGATTTCCA
    GGAGGGAAAG GTCTGCCGGG GGAGCCTGGA AGAAATGGAT CACCTGGCAG ACTTGGACCT
    CAAGGCAACA GAGGTGACAG AGGGCTTCCA GGTCTTCCAG GCCCCCCCAG TATTTACACA
    CCAGTTGTGG CGAAGGGAGA ACTGGGAGAT CCTGGAGCCA GAGGGTTTGA TGGTTTTCCT
    GGACCCAGAG GTGACAAGGG TCTGCCTGGC CTGCCCGGTC GACAAGGTCG CCCTGGTCTT
    CCTGGTCTAC CTGAGCAAAG TAAAGGGCAA CCCGGGCAGC CGGGCCTACC TGGACTGCCG
    GGATCTCCAG GCTTACCGGG ACCAAAGGGA GAAGTTGGAA TCAATGGGTT CCCTGGCATG
    CCAGGACCAA GAGGAGATGA TGGAATACCT GGTGCACCCG GAGGTCCTGG AGAACCAGGT
    CGTCCAGGTG GAAAAGGTCA ACCTGGGGAA ACCTTTGGTT TTCCTGGCTC ACCTGGACAG
    AAAGGTCAAC CGGGAGATCC AGGCAGCCCA GGACGTCAAG GCTTTGATGG AGCCCCCGGT
    GATAATGGCT TGTCTGGAAA CCCTGGTTTC CCTGGAATAA AAGGTGGTCC TGGTGAACCA
    GGACGGCCTG GATTACCTGG ATCTGTTGGT TTTCCAGGCC CTAAAGGGCT GTCAACATTC
    CCTGGAAGAA AAGGAGCAGA TGGGCCCCCA GGACAGCCTG GATTTCCAGG AGGTCCAGGA
    GCCAGGGGTG TGCCTGGACC TCCTGGCCTG GATGGACTCA ATGGCCTCGC TGGATCCAAA
    GGCCTTCCTG GAACCCCAGG TATTACTTTA GGAGGTCGCC CTGGTGCTCC TGGTATCCCA
    GGATTGAAGG GAGAGAGGGG AGATTCTTAT CCTGGACAAC GAGGCTTTGA TGGAGTAAAA
    GGACAAAAAG GAGAGCCTGG TCTTCCTGGA ACTTCAGGAA TTCCTGGTCA ACCTGGACTT
    CCTGGAGAGT CAGCGTTAGC AGGCATTGCT GGACCTGTGG GGGACCCAGG ACTTCCTGGA
    TTGGATGGAA ACTATGGTTT TCAAGGTCCA CCTGGTCCTC CTGGTCCCCC GGGTTCAGGA
    ACAATACAAG GAGAAAGAGG TGAACCAGGG CTGCCAGGCT TCCCGGGATC TCCTGGAAGA
    AAGGGAGAAC CCGGTTTCAA TGGAGGCCCT GGATACCCAG GTAGCGCCGG TGTCAAAGGA
    TCTCCTGGTG AAAGTGGTTT TAATGGACGT CCAGGTCTTA AAGGTGTGCC TGGAGATCCT
    GGCTTCTATG GAATCAAGGG ATCTAAAGGA ACACCAGGCC AAAAAGGACG GAAGGGTCCT
    CCTGGAGTTG TGCTGCCTTC CCCTATTGTG GAATCACAGC CTTCTGGCGA CATGGGTCTT
    CCTGGTGAAA GGGGACCCTT GGGTTACCGT GGAGACTCTG GTTGGAGTGG CATTCCAGGG
    CGTCCTGGTC CAAAAGGACG ACCCGGAGCC CCAGGTAACC TTGGAAGGAC TGGACCGTCT
    GGTCTGCCTG GTCCGTTAGG TGATCCCGGC TCCCCTGGTT TACCTGGAAC AAGTGGAAGA
    CAAGGCCCCC CTGGAAACAT TGGCCTCCCT GGCATTCCTG GCAGCTCCAG CCGCAGTATC
    AGCATTGGCT ACACTCTGGT AAAGCACAGC CAGAGCGAGC AGGTCCCCAT GTGTCCTCAG
    GGCATGCCTT CATTGTGGAA TGGGTACAGC TTGCTGTATG TGGAGGGACA GGAAAAAGCA
    CACAACCAAG ACCTGGGTCA GCCTGGCTCG TGCCTCCCCA GGTTCAGCAC CATGCCCTTC
    CTGTACTGCT CCCCCAATGA GGTTTGCTTC TTTGCTAGTC GCAATGACAA ATCCTACTGG
    CTCTCCACAT CTGCACCCAT TCCCATGATG CCCGTGTCTG CGGGGCAGAT CCAGCCTTAC
    ATCAGCAGAT GTTCAGTATG TGAGGCACCA TCTCAGGCTG TGGCGGTCCA CAGTCAGGAC
    ATGACCATCC CCATGTGCCC CCCTGGGTGG AGAAGTCTTT GGATTGGATA TTCCTTCTTG
    ATGCACACGG CGGCTGGTGC AGAAGGCGGC GGTCAGTCCC TGATGTCTCC CGGCTCCTGC
    TTGGAGGATT TCCGCGCCAC TCCATTCATT GAGTGCAATG GAGCCAAAGG CACATGCCAC
    TACTTTGCCA ACAAATACAG CTTCTGGCTG ACCACCGTGG ATCCCAAGCG GGAGTTTGTG
    TCTTCACCAA ACCAGGAGAC CCTGAAGGCG GGCCAGGAGC GCTCCAGAGT CAGTCGCTGT
    CAGGTCTGCA GCAAACTGCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020567397.2
    CDS480..5603
    Translation

    Target ORF information:

    RefSeq Version XM_020711738.2
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_020711738.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGTGACGTG GGTTTGCCCG GTGAGCCCGG CCAGCCTCTC
    ATCAATGGGA TTGTGGAGTT TGTGGGTTAT CCCAAAGGAG ACAAAGGAGA GCAGGGGGAG
    CCAGGTCCAA AAGGATCAAT TGGATTTCAA GGATTACCTG GACTGCCTGG AGCCTTTGGT
    TATCCAGGTG AAATTGGAGA GAAAGGCATC CCTGGATATC CTGGAGGAAG AGGTTCTCCA
    GGTTTTAATG GACCTCAGGG TGCTCCAGGA TTACCGGGAC AGAGAGGAGA CACAGGTTAC
    TCCGGCAGGC CAGGTTACGA CGGGCCAAAG GGCGACCCAG GAGACCGAGG TCCTCGAGGT
    CCTCCTATTT ATGTCAGTAA TATTGGCAGA ACTGCTCAAG GTCCTTCAGG AGATCCTGGT
    TACAGCGGTG CTCCTGGCGC TCCCGGAGAC CCAGGACCCA CAGGTTTCCC AGGACTTCCA
    GGTGAACCTG GACGTCTTCT GCCTGGTTCA GAAACATCAG GACTTCCAGG TCTACCCGGA
    CTACCTGGCC CGGAAGGAGA GAAGGGAAAC CCAGGTCCAC TGGTTAATGG TCCTGAAGGA
    TTCCCAGGTC TGCCTGGACC CCCTGGAGGT CCTGGCCCCC CGGGTCCTCC TGGCAGGACC
    ACTGATCCCT GTGTCATCTA TCCTGTCAGT CCTGAAGGCT CTCCTGAGGA TGTGACCTCC
    GGTCTACCCG GAGTACCCGG GCAGCCTGGC CCCAGAGGAC CTCCTGGAGA CCGAGGTTTC
    AAAGGCTTCG GAGGTGACCC TGGTGACTGT GACTGTGAGG GAGGGGGCTC CTCAGGGGCA
    ACTGGGTTAC CTGGACCTCC TGGACTGAAT GGCAGCCCTG GTGCCACTGG AAGGAAGGGT
    GAACAGGGTG ACACTGGATC ATCGGGGTTC AGCGGGCCTT CTGGTTCTGA TGGGCGGCCT
    GGTAGCAGGG GCTTCTTCGG TTTGAAAGGA GAAAAGGGGG AACCATATTT ATCACGGGGC
    TCTTCGAGCA AGGGGGATCG TGGAGCACCC GGTCCCAGAG GACCCATAGG TGAAACTGGA
    AGACCTGGCA GAGATGGGCT GCCTGGATTT GCTGGACTTC CTGGAAGTCC TGGCGACCCT
    GGCTTTGGTA CAGTTGGAGA AAAGGGATTC TTAGGGTTCC CGGGAATCAA AGGTCGTCCT
    GGAGCCCCTG GAGAGTCCGG CATTGGCTAC ATTGGACTGC CTGGCTTGCG AGGAGAGCCT
    GGAGACCCAG GTTTTTCTGG GTTGCCTGGG CCACCAGGTT TACCTGGACC AAGAGGTGAA
    AGCGCCTGTG GACGGGTTAA TGGCGCCGAA ACGGGAACGG GTGATTTGTT GCCCTGCAAA
    ATACCTGGAC AAGTCGGAGA CCCTGGGATT CCTGGCTTAC CTGGACGCCC TGGCCTTAAG
    GGTCAGCCGG GTCGGCCGGG AGAACCTGGA CGTCCAGGAC TTGATGGTTC CAAAGGGGAG
    AGGGGAGATC CTGGCTTTGG GAACCAGCCC GGTTCTCCAG GTTTTCCAGG ACCTAGAGGG
    GACCCAGGAA TCCCAGGTGT TCCTGGGCTG CGCATTGATG GGACCAGGGG AAGAAATGGT
    GTTCCAGGGC TTCCTGGAGC CAAAGGACAG CCTGGAGAGA TTCTTGGAGC CATACCTGGA
    CTTCCAGGAC GAGATGGTGA ACCTGGAATA CGTGGGGACA AGGGCCAGCC CGGCTCTCCA
    GGAGTTCCTG GAGGTCCTGG TCTGGATGGA AAGAACGGTA TTCCTGGACT GAGAGGAGAA
    CGTGGAATTG ATGGTCTGCC GGGTGTTCCT GGTTTTCCTG GACCTCCGGG TGAGGTTATC
    GGCCCTACTC TTGGACTGCC TGGACCTCCT GGCAGCAAAG GACAGATTGG ATCACCGGGT
    CTTCCTGGGG AGAAAGGAGA CACAGGGGAT CCAGGTTTCC CAGGACCTGC AGGAATGAAA
    GGAACACCTG GGTTTCCTGG TACTCCTGGA ACACCAGGTC CACGGGGACC GCCTGGACCT
    CCAGGACCTG GAACTGTTGA CGGATTACCT GGAATTCCTG GAGTAAAAGG GGAAAGAGGT
    TTTGTTGGAC TCACTGGTTT TCCTGGACAG TCTGGACGGC CCGGAACACC AGGATTTCCA
    GGAGGGAAAG GTCTGCCGGG GGAGCCTGGA AGAAATGGAT CACCTGGCAG ACTTGGACCT
    CAAGGCAACA GAGGTGACAG AGGGCTTCCA GGTCTTCCAG GCCCCCCCAG TATTTACACA
    CCAGTTGTGG CGAAGGGAGA ACTGGGAGAT CCTGGAGCCA GAGGGTTTGA TGGTTTTCCT
    GGACCCAGAG GTGACAAGGG TCTGCCTGGC CTGCCCGGTC GACAAGGTCG CCCTGGTCTT
    CCTGGTCTAC CTGAGCAAAG TAAAGGGCAA CCCGGGCAGC CGGGCCTACC TGGACTGCCG
    GGATCTCCAG GCTTACCGGG ACCAAAGGGA GAAGTTGGAA TCAATGGGTT CCCTGGCATG
    CCAGGACCAA GAGGAGATGA TGGAATACCT GGTGCACCCG GAGGTCCTGG AGAACCAGGT
    CGTCCAGGTG GAAAAGGTCA ACCTGGGGAA ACCTTTGGTT TTCCTGGCTC ACCTGGACAG
    AAAGGTCAAC CGGGAGATCC AGGCAGCCCA GGACGTCAAG GCTTTGATGG AGCCCCCGGT
    GATAATGGCT TGTCTGGAAA CCCTGGTTTC CCTGGAATAA AAGGTGGTCC TGGTGAACCA
    GGACGGCCTG GATTACCTGG ATCTGTTGGT TTTCCAGGCC CTAAAGGGCT GTCAACATTC
    CCTGGAAGAA AAGGAGCAGA TGGGCCCCCA GGACAGCCTG GATTTCCAGG AGGTCCAGGA
    GCCAGGGGTG TGCCTGGACC TCCTGGCCTG GATGGACTCA ATGGCCTCGC TGGATCCAAA
    GGCCTTCCTG GAACCCCAGG TATTACTTTA GGAGGTCGCC CTGGTGCTCC TGGTATCCCA
    GGATTGAAGG GAGAGAGGGG AGATTCTTAT CCTGGACAAC GAGGCTTTGA TGGAGTAAAA
    GGACAAAAAG GAGAGCCTGG TCTTCCTGGA ACTTCAGGAA TTCCTGGTCA ACCTGGACTT
    CCTGGAGAGT CAGCGTTAGC AGGCATTGCT GGACCTGTGG GGGACCCAGG ACTTCCTGGA
    TTGGATGGAA ACTATGGTTT TCAAGGTCCA CCTGGTCCTC CTGGTCCCCC GGGTTCAGGA
    ACAATACAAG GAGAAAGAGG TGAACCAGGG CTGCCAGGCT TCCCGGGATC TCCTGGAAGA
    AAGGGAGAAC CCGGTTTCAA TGGAGGCCCT GGATACCCAG GTAGCGCCGG TGTCAAAGGA
    TCTCCTGGTG AAAGTGGTTT TAATGGACGT CCAGGTCTTA AAGGTGTGCC TGGAGATCCT
    GGCTTCTATG GAATCAAGGG ATCTAAAGGA ACACCAGGCC AAAAAGGACG GAAGGGTCCT
    CCTGGAGTTG TGCTGCCTTC CCCTATTGTG GAATCACAGC CTTCTGGCGA CATGGGTCTT
    CCTGGTGAAA GGGGACCCTT GGGTTACCGT GGAGACTCTG GTTGGAGTGG CATTCCAGGG
    CGTCCTGGTC CAAAAGGACG ACCCGGAGCC CCAGGTAACC TTGGAAGGAC TGGACCGTCT
    GGTCTGCCTG GTCCGTTAGG TGATCCCGGC TCCCCTGGTT TACCTGGAAC AAGTGGAAGA
    CAAGGCCCCC CTGGAAACAT TGGCCTCCCT GGCATTCCTG GCAGCTCCAG CCGCAGTATC
    AGCATTGGCT ACACTCTGGT AAAGCACAGC CAGAGCGAGC AGGTCCCCAT GTGTCCTCAG
    GGCATGCCTT CATTGTGGAA TGGGTACAGC TTGCTGTATG TGGAGGGACA GGAAAAAGCA
    CACAACCAAG ACCTGGGTCA GCCTGGCTCG TGCCTCCCCA GGTTCAGCAC CATGCCCTTC
    CTGTACTGCT CCCCCAATGA GGTTTGCTTC TTTGCTAGTC GCAATGACAA ATCCTACTGG
    CTCTCCACAT CTGCACCCAT TCCCATGATG CCCGTGTCTG CGGGGCAGAT CCAGCCTTAC
    ATCAGCAGAT GTTCAGTATG TGAGGCACCA TCTCAGGCTG TGGCGGTCCA CAGTCAGGAC
    ATGACCATCC CCATGTGCCC CCCTGGGTGG AGAAGTCTTT GGATTGGATA TTCCTTCTTG
    ATGCACACGG CGGCTGGTGC AGAAGGCGGC GGTCAGTCCC TGATGTCTCC CGGCTCCTGC
    TTGGAGGATT TCCGCGCCAC TCCATTCATT GAGTGCAATG GAGCCAAAGG CACATGCCAC
    TACTTTGCCA ACAAATACAG CTTCTGGCTG ACCACCGTGG ATCCCAAGCG GGAGTTTGTG
    TCTTCACCAA ACCAGGAGAC CCTGAAGGCG GGCCAGGAGC GCTCCAGAGT CAGTCGCTGT
    CAGGTCTGCA GCAAACTGCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa111309
    Clone ID Related Accession (Same CDS sequence) XM_020711740.2
    Accession Version XM_020711740.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1518537600000
    Organism Oryzias latipes(Japanese medaka)
    Product collagen alpha-6(IV) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_020711740.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGTGACGTG GGTTTGCCCG GTGAGCCCGG CCAGCCTCTC
    ATCAATGGGA TTGTGGAGTT TGTGGGTTAT CCCAAAGGAG ACAAAGGAGA GCAGGGGGAG
    CCAGGTCCAA AAGGATCAAT TGGATTTCAA GGATTACCTG GACTGCCTGG AGCCTTTGGT
    TATCCAGGTG AAATTGGAGA GAAAGGCATC CCTGGATATC CTGGAGGAAG AGGTTCTCCA
    GGTTTTAATG GACCTCAGGG TGCTCCAGGA TTACCGGGAC AGAGAGGAGA CACAGGTTAC
    TCCGGCAGGC CAGGTTACGA CGGGCCAAAG GGCGACCCAG GAGACCGAGG TCCTCGAGGT
    CCTCCTATTT ATGTCAGTAA TATTGGCAGA ACTGCTCAAG GTCCTTCAGG AGATCCTGGT
    TACAGCGGTG CTCCTGGCGC TCCCGGAGAC CCAGGACCCA CAGGTTTCCC AGGACTTCCA
    GGTGAACCTG GACGTCTTCT GCCTGGTTCA GAAACATCAG GACTTCCAGG TCTACCCGGA
    CTACCTGGCC CGGAAGGAGA GAAGGGAAAC CCAGGTCCAC TGGTTAATGG TCCTGAAGGA
    TTCCCAGGTC TGCCTGGACC CCCTGGAGGT CCTGGCCCCC CGGGTCCTCC TGGCTCTCCT
    GAGGATGTGA CCTCCGGTCT ACCCGGAGTA CCCGGGCAGC CTGGCCCCAG AGGACCTCCT
    GGAGACCGAG GTTTCAAAGG CTTCGGAGGT GACCCTGGTG ACTGTGACTG TGAGGGAGGG
    GGCTCCTCAG GGGCAACTGG GTTACCTGGA CCTCCTGGAC TGAATGGCAG CCCTGGTGCC
    ACTGGAAGGA AGGGTGAACA GGGTGACACT GGATCATCGG GGTTCAGCGG GCCTTCTGGT
    TCTGATGGGC GGCCTGGTAG CAGGGGCTTC TTCGGTTTGA AAGGAGAAAA GGGGGAACCA
    TATTTATCAC GGGGCTCTTC GAGCAAGGGG GATCGTGGAG CACCCGGTCC CAGAGGACCC
    ATAGGTGAAA CTGGAAGACC TGGCAGAGAT GGGCTGCCTG GATTTGCTGG ACTTCCTGGA
    AGTCCTGGCG ACCCTGGCTT TGGTACAGTT GGAGAAAAGG GATTCTTAGG GTTCCCGGGA
    ATCAAAGGTC GTCCTGGAGC CCCTGGAGAG TCCGGCATTG GCTACATTGG ACTGCCTGGC
    TTGCGAGGAG AGCCTGGAGA CCCAGGTTTT TCTGGGTTGC CTGGGCCACC AGGTTTACCT
    GGACCAAGAG GTGAAAGCGC CTGTGGACGG GTTAATGGCG CCGAAACGGG AACGGGTGAT
    TTGTTGCCCT GCAAAATACC TGGACAAGTC GGAGACCCTG GGATTCCTGG CTTACCTGGA
    CGCCCTGGCC TTAAGGGTCA GCCGGGTCGG CCGGGAGAAC CTGGACGTCC AGGACTTGAT
    GGTTCCAAAG GGGAGAGGGG AGATCCTGGC TTTGGGAACC AGCCCGGTTC TCCAGGTTTT
    CCAGGACCTA GAGGGGACCC AGGAATCCCA GGTGTTCCTG GGCTGCGCAT TGATGGGACC
    AGGGGAAGAA ATGGTGTTCC AGGGCTTCCT GGAGCCAAAG GACAGCCTGG AGAGATTCTT
    GGAGCCATAC CTGGACTTCC AGGACGAGAT GGTGAACCTG GAATACGTGG GGACAAGGGC
    CAGCCCGGCT CTCCAGGAGT TCCTGGAGGT CCTGGTCTGG ATGGAAAGAA CGGTATTCCT
    GGACTGAGAG GAGAACGTGG AATTGATGGT CTGCCGGGTG TTCCTGGTTT TCCTGGACCT
    CCGGGTGAGG TTATCGGCCC TACTCTTGGA CTGCCTGGAC CTCCTGGCAG CAAAGGACAG
    ATTGGATCAC CGGGCTGCCC AGGTCTTCCT GGGGAGAAAG GAGACACAGG GGATCCAGGT
    TTCCCAGGAC CTGCAGGAAT GAAAGGAACA CCTGGGTTTC CTGGTACTCC TGGAACACCA
    GGTCCACGGG GACCGCCTGG ACCTCCAGGA CCTGGAACTG TTGACGGATT ACCTGGAATT
    CCTGGAGTAA AAGGGGAAAG AGGTTTTGTT GGACTCACTG GTTTTCCTGG ACAGTCTGGA
    CGGCCCGGAA CACCAGGATT TCCAGGAGGG AAAGGTCTGC CGGGGGAGCC TGGAAGAAAT
    GGATCACCTG GCAGACTTGG ACCTCAAGGC AACAGAGGTG ACAGAGGGCT TCCAGGTCTT
    CCAGGCCCCC CCAGTATTTA CACACCAGTT GTGGCGAAGG GAGAACTGGG AGATCCTGGA
    GCCAGAGGGT TTGATGGTTT TCCTGGACCC AGAGGTGACA AGGGTCTGCC TGGCCTGCCC
    GGTCGACAAG GTCGCCCTGG TCTTCCTGGT CTACCTGAGC AAAGTAAAGG GCAACCCGGG
    CAGCCGGGCC TACCTGGACT GCCGGGATCT CCAGGCTTAC CGGGACCAAA GGGAGAAGTT
    GGAATCAATG GGTTCCCTGG CATGCCAGGA CCAAGAGGAG ATGATGGAAT ACCTGGTGCA
    CCCGGAGGTC CTGGAGAACC AGGTCGTCCA GGTGGAAAAG GTCAACCTGG GGAAACCTTT
    GGTTTTCCTG GCTCACCTGG ACAGAAAGGT CAACCGGGAG ATCCAGGCAG CCCAGGACGT
    CAAGGCTTTG ATGGAGCCCC CGGTGATAAT GGCTTGTCTG GAAACCCTGG TTTCCCTGGA
    ATAAAAGGTG GTCCTGGTGA ACCAGGACGG CCTGGATTAC CTGGATCTGT TGGTTTTCCA
    GGCCCTAAAG GGCTGTCAAC ATTCCCTGGA AGAAAAGGAG CAGATGGGCC CCCAGGACAG
    CCTGGATTTC CAGGAGGTCC AGGAGCCAGG GGTGTGCCTG GACCTCCTGG CCTGGATGGA
    CTCAATGGCC TCGCTGGATC CAAAGGCCTT CCTGGAACCC CAGGTATTAC TTTAGGAGGT
    CGCCCTGGTG CTCCTGGTAT CCCAGGATTG AAGGGAGAGA GGGGAGATTC TTATCCTGGA
    CAACGAGGCT TTGATGGAGT AAAAGGACAA AAAGGAGAGC CTGGTCTTCC TGGAACTTCA
    GGAATTCCTG GTCAACCTGG ACTTCCTGGA GAGTCAGCGT TAGCAGGCAT TGCTGGACCT
    GTGGGGGACC CAGGACTTCC TGGATTGGAT GGAAACTATG GTTTTCAAGG TCCACCTGGT
    CCTCCTGGTC CCCCGGGTTC AGGAACAATA CAAGGAGAAA GAGGTGAACC AGGGCTGCCA
    GGCTTCCCGG GATCTCCTGG AAGAAAGGGA GAACCCGGTT TCAATGGAGG CCCTGGATAC
    CCAGGTAGCG CCGGTGTCAA AGGATCTCCT GGTGAAAGTG GTTTTAATGG ACGTCCAGGT
    CTTAAAGGTG TGCCTGGAGA TCCTGGCTTC TATGGAATCA AGGGATCTAA AGGAACACCA
    GGCCAAAAAG GACGGAAGGG TCCTCCTGGA GTTGTGCTGC CTTCCCCTAT TGTGGAATCA
    CAGCCTTCTG GCGACATGGG TCTTCCTGGT GAAAGGGGAC CCTTGGGTTA CCGTGGAGAC
    TCTGGTTGGA GTGGCATTCC AGGGCGTCCT GGTCCAAAAG GACGACCCGG AGCCCCAGGT
    AACCTTGGAA GGACTGGACC GTCTGGTCTG CCTGGTCCGT TAGGTGATCC CGGCTCCCCT
    GGTTTACCTG GAACAAGTGG AAGACAAGGC CCCCCTGGAA ACATTGGCCT CCCTGGCATT
    CCTGGCAGCT CCAGCCGCAG TATCAGCATT GGCTACACTC TGGTAAAGCA CAGCCAGAGC
    GAGCAGGTCC CCATGTGTCC TCAGGGCATG CCTTCATTGT GGAATGGGTA CAGCTTGCTG
    TATGTGGAGG GACAGGAAAA AGCACACAAC CAAGACCTGG GTCAGCCTGG CTCGTGCCTC
    CCCAGGTTCA GCACCATGCC CTTCCTGTAC TGCTCCCCCA ATGAGGTTTG CTTCTTTGCT
    AGTCGCAATG ACAAATCCTA CTGGCTCTCC ACATCTGCAC CCATTCCCAT GATGCCCGTG
    TCTGCGGGGC AGATCCAGCC TTACATCAGC AGATGTTCAG TATGTGAGGC ACCATCTCAG
    GCTGTGGCGG TCCACAGTCA GGACATGACC ATCCCCATGT GCCCCCCTGG GTGGAGAAGT
    CTTTGGATTG GATATTCCTT CTTGATGCAC ACGGCGGCTG GTGCAGAAGG CGGCGGTCAG
    TCCCTGATGT CTCCCGGCTC CTGCTTGGAG GATTTCCGCG CCACTCCATT CATTGAGTGC
    AATGGAGCCA AAGGCACATG CCACTACTTT GCCAACAAAT ACAGCTTCTG GCTGACCACC
    GTGGATCCCA AGCGGGAGTT TGTGTCTTCA CCAAACCAGG AGACCCTGAA GGCGGGCCAG
    GAGCGCTCCA GAGTCAGTCG CTGTCAGGTC TGCAGCAAAC TGCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020567399.2
    CDS480..5567
    Translation

    Target ORF information:

    RefSeq Version XM_020711740.2
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_020711740.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGTGACGTG GGTTTGCCCG GTGAGCCCGG CCAGCCTCTC
    ATCAATGGGA TTGTGGAGTT TGTGGGTTAT CCCAAAGGAG ACAAAGGAGA GCAGGGGGAG
    CCAGGTCCAA AAGGATCAAT TGGATTTCAA GGATTACCTG GACTGCCTGG AGCCTTTGGT
    TATCCAGGTG AAATTGGAGA GAAAGGCATC CCTGGATATC CTGGAGGAAG AGGTTCTCCA
    GGTTTTAATG GACCTCAGGG TGCTCCAGGA TTACCGGGAC AGAGAGGAGA CACAGGTTAC
    TCCGGCAGGC CAGGTTACGA CGGGCCAAAG GGCGACCCAG GAGACCGAGG TCCTCGAGGT
    CCTCCTATTT ATGTCAGTAA TATTGGCAGA ACTGCTCAAG GTCCTTCAGG AGATCCTGGT
    TACAGCGGTG CTCCTGGCGC TCCCGGAGAC CCAGGACCCA CAGGTTTCCC AGGACTTCCA
    GGTGAACCTG GACGTCTTCT GCCTGGTTCA GAAACATCAG GACTTCCAGG TCTACCCGGA
    CTACCTGGCC CGGAAGGAGA GAAGGGAAAC CCAGGTCCAC TGGTTAATGG TCCTGAAGGA
    TTCCCAGGTC TGCCTGGACC CCCTGGAGGT CCTGGCCCCC CGGGTCCTCC TGGCTCTCCT
    GAGGATGTGA CCTCCGGTCT ACCCGGAGTA CCCGGGCAGC CTGGCCCCAG AGGACCTCCT
    GGAGACCGAG GTTTCAAAGG CTTCGGAGGT GACCCTGGTG ACTGTGACTG TGAGGGAGGG
    GGCTCCTCAG GGGCAACTGG GTTACCTGGA CCTCCTGGAC TGAATGGCAG CCCTGGTGCC
    ACTGGAAGGA AGGGTGAACA GGGTGACACT GGATCATCGG GGTTCAGCGG GCCTTCTGGT
    TCTGATGGGC GGCCTGGTAG CAGGGGCTTC TTCGGTTTGA AAGGAGAAAA GGGGGAACCA
    TATTTATCAC GGGGCTCTTC GAGCAAGGGG GATCGTGGAG CACCCGGTCC CAGAGGACCC
    ATAGGTGAAA CTGGAAGACC TGGCAGAGAT GGGCTGCCTG GATTTGCTGG ACTTCCTGGA
    AGTCCTGGCG ACCCTGGCTT TGGTACAGTT GGAGAAAAGG GATTCTTAGG GTTCCCGGGA
    ATCAAAGGTC GTCCTGGAGC CCCTGGAGAG TCCGGCATTG GCTACATTGG ACTGCCTGGC
    TTGCGAGGAG AGCCTGGAGA CCCAGGTTTT TCTGGGTTGC CTGGGCCACC AGGTTTACCT
    GGACCAAGAG GTGAAAGCGC CTGTGGACGG GTTAATGGCG CCGAAACGGG AACGGGTGAT
    TTGTTGCCCT GCAAAATACC TGGACAAGTC GGAGACCCTG GGATTCCTGG CTTACCTGGA
    CGCCCTGGCC TTAAGGGTCA GCCGGGTCGG CCGGGAGAAC CTGGACGTCC AGGACTTGAT
    GGTTCCAAAG GGGAGAGGGG AGATCCTGGC TTTGGGAACC AGCCCGGTTC TCCAGGTTTT
    CCAGGACCTA GAGGGGACCC AGGAATCCCA GGTGTTCCTG GGCTGCGCAT TGATGGGACC
    AGGGGAAGAA ATGGTGTTCC AGGGCTTCCT GGAGCCAAAG GACAGCCTGG AGAGATTCTT
    GGAGCCATAC CTGGACTTCC AGGACGAGAT GGTGAACCTG GAATACGTGG GGACAAGGGC
    CAGCCCGGCT CTCCAGGAGT TCCTGGAGGT CCTGGTCTGG ATGGAAAGAA CGGTATTCCT
    GGACTGAGAG GAGAACGTGG AATTGATGGT CTGCCGGGTG TTCCTGGTTT TCCTGGACCT
    CCGGGTGAGG TTATCGGCCC TACTCTTGGA CTGCCTGGAC CTCCTGGCAG CAAAGGACAG
    ATTGGATCAC CGGGCTGCCC AGGTCTTCCT GGGGAGAAAG GAGACACAGG GGATCCAGGT
    TTCCCAGGAC CTGCAGGAAT GAAAGGAACA CCTGGGTTTC CTGGTACTCC TGGAACACCA
    GGTCCACGGG GACCGCCTGG ACCTCCAGGA CCTGGAACTG TTGACGGATT ACCTGGAATT
    CCTGGAGTAA AAGGGGAAAG AGGTTTTGTT GGACTCACTG GTTTTCCTGG ACAGTCTGGA
    CGGCCCGGAA CACCAGGATT TCCAGGAGGG AAAGGTCTGC CGGGGGAGCC TGGAAGAAAT
    GGATCACCTG GCAGACTTGG ACCTCAAGGC AACAGAGGTG ACAGAGGGCT TCCAGGTCTT
    CCAGGCCCCC CCAGTATTTA CACACCAGTT GTGGCGAAGG GAGAACTGGG AGATCCTGGA
    GCCAGAGGGT TTGATGGTTT TCCTGGACCC AGAGGTGACA AGGGTCTGCC TGGCCTGCCC
    GGTCGACAAG GTCGCCCTGG TCTTCCTGGT CTACCTGAGC AAAGTAAAGG GCAACCCGGG
    CAGCCGGGCC TACCTGGACT GCCGGGATCT CCAGGCTTAC CGGGACCAAA GGGAGAAGTT
    GGAATCAATG GGTTCCCTGG CATGCCAGGA CCAAGAGGAG ATGATGGAAT ACCTGGTGCA
    CCCGGAGGTC CTGGAGAACC AGGTCGTCCA GGTGGAAAAG GTCAACCTGG GGAAACCTTT
    GGTTTTCCTG GCTCACCTGG ACAGAAAGGT CAACCGGGAG ATCCAGGCAG CCCAGGACGT
    CAAGGCTTTG ATGGAGCCCC CGGTGATAAT GGCTTGTCTG GAAACCCTGG TTTCCCTGGA
    ATAAAAGGTG GTCCTGGTGA ACCAGGACGG CCTGGATTAC CTGGATCTGT TGGTTTTCCA
    GGCCCTAAAG GGCTGTCAAC ATTCCCTGGA AGAAAAGGAG CAGATGGGCC CCCAGGACAG
    CCTGGATTTC CAGGAGGTCC AGGAGCCAGG GGTGTGCCTG GACCTCCTGG CCTGGATGGA
    CTCAATGGCC TCGCTGGATC CAAAGGCCTT CCTGGAACCC CAGGTATTAC TTTAGGAGGT
    CGCCCTGGTG CTCCTGGTAT CCCAGGATTG AAGGGAGAGA GGGGAGATTC TTATCCTGGA
    CAACGAGGCT TTGATGGAGT AAAAGGACAA AAAGGAGAGC CTGGTCTTCC TGGAACTTCA
    GGAATTCCTG GTCAACCTGG ACTTCCTGGA GAGTCAGCGT TAGCAGGCAT TGCTGGACCT
    GTGGGGGACC CAGGACTTCC TGGATTGGAT GGAAACTATG GTTTTCAAGG TCCACCTGGT
    CCTCCTGGTC CCCCGGGTTC AGGAACAATA CAAGGAGAAA GAGGTGAACC AGGGCTGCCA
    GGCTTCCCGG GATCTCCTGG AAGAAAGGGA GAACCCGGTT TCAATGGAGG CCCTGGATAC
    CCAGGTAGCG CCGGTGTCAA AGGATCTCCT GGTGAAAGTG GTTTTAATGG ACGTCCAGGT
    CTTAAAGGTG TGCCTGGAGA TCCTGGCTTC TATGGAATCA AGGGATCTAA AGGAACACCA
    GGCCAAAAAG GACGGAAGGG TCCTCCTGGA GTTGTGCTGC CTTCCCCTAT TGTGGAATCA
    CAGCCTTCTG GCGACATGGG TCTTCCTGGT GAAAGGGGAC CCTTGGGTTA CCGTGGAGAC
    TCTGGTTGGA GTGGCATTCC AGGGCGTCCT GGTCCAAAAG GACGACCCGG AGCCCCAGGT
    AACCTTGGAA GGACTGGACC GTCTGGTCTG CCTGGTCCGT TAGGTGATCC CGGCTCCCCT
    GGTTTACCTG GAACAAGTGG AAGACAAGGC CCCCCTGGAA ACATTGGCCT CCCTGGCATT
    CCTGGCAGCT CCAGCCGCAG TATCAGCATT GGCTACACTC TGGTAAAGCA CAGCCAGAGC
    GAGCAGGTCC CCATGTGTCC TCAGGGCATG CCTTCATTGT GGAATGGGTA CAGCTTGCTG
    TATGTGGAGG GACAGGAAAA AGCACACAAC CAAGACCTGG GTCAGCCTGG CTCGTGCCTC
    CCCAGGTTCA GCACCATGCC CTTCCTGTAC TGCTCCCCCA ATGAGGTTTG CTTCTTTGCT
    AGTCGCAATG ACAAATCCTA CTGGCTCTCC ACATCTGCAC CCATTCCCAT GATGCCCGTG
    TCTGCGGGGC AGATCCAGCC TTACATCAGC AGATGTTCAG TATGTGAGGC ACCATCTCAG
    GCTGTGGCGG TCCACAGTCA GGACATGACC ATCCCCATGT GCCCCCCTGG GTGGAGAAGT
    CTTTGGATTG GATATTCCTT CTTGATGCAC ACGGCGGCTG GTGCAGAAGG CGGCGGTCAG
    TCCCTGATGT CTCCCGGCTC CTGCTTGGAG GATTTCCGCG CCACTCCATT CATTGAGTGC
    AATGGAGCCA AAGGCACATG CCACTACTTT GCCAACAAAT ACAGCTTCTG GCTGACCACC
    GTGGATCCCA AGCGGGAGTT TGTGTCTTCA CCAAACCAGG AGACCCTGAA GGCGGGCCAG
    GAGCGCTCCA GAGTCAGTCG CTGTCAGGTC TGCAGCAAAC TGCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa31759
    Clone ID Related Accession (Same CDS sequence) XM_011487658.2
    Accession Version XM_011487658.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1491408000000
    Organism Oryzias latipes(Japanese medaka)
    Product collagen alpha-6(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011487658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGCGACGTG GGTCTTCCCG GACCCAGTGG TGCTCCCGGA
    AGCGGGTCTC TAACGGCCCC AAAAATTCTC ACTATCTACA AGGGAGATAA GGGGGAGCCA
    GGTCCAAAAG GATCAATTGG ATTTCAAGGA TTACCTGGAC TGCCTGGAGC CTTTGGTTAT
    CCAGGTGAAA TTGGAGAGAA AGGCATCCCT GGATATCCTG GAGGAAGAGG TTCTCCAGGT
    TTTAATGGAC CTCAGGGTGC TCCAGGATTA CCGGGACAGA GAGGAGACAC AGGTTACTCC
    GGCAGGCCAG GTTACGACGG GCCAAAGGGC GACCCAGGAG ACCGAGGTCC TCGAGGTCCT
    CCTATTTATG TCAGTAATAT TGGCAGAACT GCTCAAGGTC CTTCAGGAGA TCCTGGTTAC
    AGCGGTGCTC CTGGCGCTCC CGGAGACCCA GGACCCACAG GTTTCCCAGG ACTTCCAGGT
    GAACCTGGAC GTCTTCTGCC TGGTTCAGAA ACATCAGGAC TTCCAGGTCT ACCCGGACTA
    CCTGGCCCGG AAGGAGAGAA GGGAAACCCA GGTCCACTGG TTAATGGTCC TGAAGGATTC
    CCAGGTCTGC CTGGACCCCC TGGAGGTCCT GGCCCCCCGG GTCCTCCTGG CAGGACCACT
    GATCCCTGTG TCATCTATCC TGTCAGTCCT GAAGGCTCTC CTGAGGATGT GACCTCCGGT
    CTACCCGGAG TACCCGGGCA GCCTGGCCCC AGAGGACCTC CTGGAGACCG AGGTTTCAAA
    GGCTTCGGAG GTGACCCTGG TGACTGTGAC TGTGAGGGAG GGGGCTCCTC AGGGGCAACT
    GGGTTACCTG GACCTCCTGG ACTGAATGGC AGCCCTGGTG CCACTGGAAG GAAGGGTGAA
    CAGGGTGACA CTGGATCATC GGGGTTCAGC GGGCCTTCTG GTTCTGATGG GCGGCCTGGT
    AGCAGGGGCT TCTTCGGTTT GAAAGGAGAA AAGGGGGAAC CATATTTATC ACGGGGCTCT
    TCGAGCAAGG GGGATCGTGG AGCACCCGGT CCCAGAGGAC CCATAGGTGA AACTGGAAGA
    CCTGGCAGAG ATGGGCTGCC TGGATTTGCT GGACTTCCTG GAAGTCCTGG CGACCCTGGC
    TTTGGTACAG TTGGAGAAAA GGGATTCTTA GGGTTCCCGG GAATCAAAGG TCGTCCTGGA
    GCCCCTGGAG AGTCCGGCAT TGGCTACATT GGACTGCCTG GCTTGCGAGG AGAGCCTGGA
    GACCCAGGTT TTTCTGGGTT GCCTGGGCCA CCAGGTTTAC CTGGACCAAG AGGTGAAAGC
    GCCTGTGGAC GGGTTAATGG CGCCAAAACG GGAACGGGTG ATTTGTTGCC CTGCAAAATA
    CCTGGACAAG TCGGAGACCC TGGGATTCCT GGCTTACCTG GACGCCCTGG CCTTAAGGGT
    CAGCCGGGTC GGCCGGGAGA ACCTGGACGT CCAGGACTTG ATGGTTCCAA AGGGGAGAGG
    GGAGATCCTG GCTTTGGGAA CCAGCCCGGT TCTCCAGGTT TTCCAGGACC TAGAGGGGAC
    CCAGGAATCC CAGGTGTTCC TGGGCTGCGC ATTGATGGGA CCAGGGGAAG AAATGGTGTT
    CCAGGGCTTC CTGGAGCCAA AGGACAGCCT GGAGAGATTC TTGGAGCCAT ACCTGGACTT
    CCAGGACGAG ATGGTGAACC TGGAATACGT GGGGACAAGG GCCAGCCCGG CTCTCCAGGA
    GTTCCTGGAG GTCCTGGTCT GGATGGAAAG AACGGTATTC CTGGACTGAG AGGAGAACGT
    GGAATTGATG GTCTGCCGGG TGTTCCTGGT TTTCCTGGAC CTCCGGGTGA GGTTATCGGC
    CCTACTCTTG GACTGCCTGG ACCTCCTGGC AGCAAAGGAC AGATTGGATC ACCGGGCTGC
    CCAGGTCTTC CTGGGGAGAA AGGAGACACA GGGGATCCAG GTTTCCCAGG ACCTGCAGGA
    ATGAAAGGAA CACCTGGGTT TCCTGGTACT CCTGGAACAC CAGGTCCACG GGGACCGCCT
    GGACCTCCAG GACCTGGAAC TGTTGACGGA TTACCTGGAA TTCCTGGAGT AAAAGGGGAA
    AGAGGTTTTG TTGGACTCAC TGGTTTTCCT GGACAGTCTG GACGGCCCGG AACACCAGGA
    TTTCCAGGAG GGAAAGGTCT GCCGGGGGAG CCTGGAAGAA ATGGATCACC TGGCAGACTT
    GGACCTCAAG GCAACAGAGG TGACAGAGGG CTTCCAGGTC TTCCAGGCCC CCCCAGTATT
    TACACACCAG TTGTGGCGAA GGGAGAACTG GGAGATCCTG GAGCCAGAGG GTTTGATGGT
    TTTCCTGGAC CCAGAGGTGA CAAGGGTCTG CCTGGCCTGC CCGGTCGACA AGGTCGCCCT
    GGTCTTCCTG GTCTACCTGA GCAAAGTAAA GGGCAACCCG GGCAGCCGGG CCTACCTGGA
    CTGCCGGGAT CTCCAGGCTT ACCGGGACCA AAGGGAGAAG TTGGAATCAA TGGGTTCCCT
    GGCATGCCAG GACCAAGAGG AGATGATGGA ATACCTGGTG CACCCGGAGG TCCTGGAGAA
    CCAGGTCGTC CAGGTGGAAA AGGTCAACCT GGGGAAACCT TTGGTTTTCC TGGCTCACCT
    GGACAGAAAG GTCAACCGGG AGATCCAGGC AGCCCAGGAC GTCAAGGCTT TGATGGAGCC
    CCCGGTGATA ATGGCTTGTC TGGAAACCCT GGTTTCCCTG GAATAAAAGG TGGTCCTGGT
    GAACCAGGAC GGCCTGGATT ACCTGGATCT GTTGGTTTTC CAGGCCCTAA AGGGCTGTCA
    ACATTCCCTG GAAGAAAAGG AGCAGATGGG CCCCCAGGAC AGCCTGGATT TCCAGGAGGT
    CCAGGAGCCA GGGGTGTGCC TGGACCTCCT GGCCTGGATG GACTCAATGG CCTCGCTGGA
    TCCAAAGGCC TTCCTGGAAC CCCAGGTATT ACTTTAGGAG GTCGCCCTGG TGCTCCTGGT
    ATCCCAGGAT TGAAGGGAGA GAGGGGAGAT TCTTATCCTG GACAACGAGG CTTTGATGGA
    GTAAAAGGAC AAAAAGGAGA GCCTGGTCTT CCTGGAACTT CAGGAATTCC TGGTCAACCT
    GGACTTCCTG GAGAGTCAGC GTTAGCAGGC ATTGCTGGAC CTGTGGGGGA CCCAGGACTT
    CCTGGATTGG ATGGAAACTA TGGTTTTCAA GGTCCACCTG GTCCTCCTGG TCCCCCGGGT
    TCAGGAACAA TACAAGGAGA AAGAGGTGAA CCAGGGCTGC CAGGCTTCCC GGGATCTCCT
    GGAAGAAAGG GAGAACCCGG TTTCAATGGA GGCCCTGGAT ACCCAGGTAG CGCCGGTGTC
    AAAGGATCTC CTGGTGAAAG TGGTTTTAAT GGACGTCCAG GTCTTAAAGG TGTGCCTGGA
    GATCCTGGCT TCTATGGAAT CAAGGGATCT AAAGGAACAC CAGGCCAAAA AGGACGGAAG
    GGTCCTCCTG GAGTTGTGCT GCCTTCCCCT ATTGTGGAAT CACAGCCTTC TGGCGACATG
    GGTCTTCCTG GTGAAAGGGG ACCCTTGGGT TACCGTGGAG ACTCTGGTTG GAGTGGCATT
    CCAGGGCGTC CTGGTCCAAA AGGACGACCC GGAGCCCCAG GTAACCTTGG AAGGACTGGA
    CCGTCTGGTC TGCCTGGTCC GTTAGGTGAT CCCGGCTCCC CTGGTTTACC TGGAACAAGT
    GGAAGACAAG GCCCCCCTGG AAACATTGGC CTCCCTGGCA TTCCTGGCAG CTCCAGCCGC
    AGTATCAGCA TTGGCTACAC TCTGGTAAAG CACAGCCAGA GCGAGCAGGT CCCCATGTGT
    CCTCAGGGCA TGCCTTCATT GTGGAATGGG TACAGCTTGC TGTATGTGGA GGGACAGGAA
    AAAGCACACA ACCAAGACCT GGGTCAGCCT GGCTCGTGCC TCCCCAGGTT CAGCACCATG
    CCCTTCCTGT ACTGCTCCCC CAATGAGGTT TGCTTCTTTG CTAGTCGCAA TGACAAATCC
    TACTGGCTCT CCACATCTGC ACCCATTCCC ATGATGCCCG TGTCTGCGGG GCAGATCCAG
    CCTTACATCA GCAGATGTTC AGTATGTGAG GCACCATCTC AGGCTGTGGC GGTCCACAGT
    CAGGACATGA CCATCCCCAT GTGCCCCCCT GGGTGGAGAA GTCTTTGGAT TGGATATTCC
    TTCTTGATGC ACACGGCGGC TGGTGCAGAA GGCGGCGGTC AGTCCCTGAT GTCTCCCGGC
    TCCTGCTTGG AGGATTTCCG CGCCACTCCA TTCATTGAGT GCAATGGAGC CAAAGGCACA
    TGCCACTACT TTGCCAACAA ATACAGCTTC TGGCTGACCA CCGTGGATCC CAAGCGGGAG
    TTTGTGTCTT CACCAAACCA GGAGACCCTG AAGGCGGGCC AGGAGCGCTC CAGAGTCAGT
    CGCTGTCAGG TCTGCAGCAA ACTGCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011485960.1
    CDS488..5617
    Translation

    Target ORF information:

    RefSeq Version XM_011487658.2
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011487658.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGCGACGTG GGTCTTCCCG GACCCAGTGG TGCTCCCGGA
    AGCGGGTCTC TAACGGCCCC AAAAATTCTC ACTATCTACA AGGGAGATAA GGGGGAGCCA
    GGTCCAAAAG GATCAATTGG ATTTCAAGGA TTACCTGGAC TGCCTGGAGC CTTTGGTTAT
    CCAGGTGAAA TTGGAGAGAA AGGCATCCCT GGATATCCTG GAGGAAGAGG TTCTCCAGGT
    TTTAATGGAC CTCAGGGTGC TCCAGGATTA CCGGGACAGA GAGGAGACAC AGGTTACTCC
    GGCAGGCCAG GTTACGACGG GCCAAAGGGC GACCCAGGAG ACCGAGGTCC TCGAGGTCCT
    CCTATTTATG TCAGTAATAT TGGCAGAACT GCTCAAGGTC CTTCAGGAGA TCCTGGTTAC
    AGCGGTGCTC CTGGCGCTCC CGGAGACCCA GGACCCACAG GTTTCCCAGG ACTTCCAGGT
    GAACCTGGAC GTCTTCTGCC TGGTTCAGAA ACATCAGGAC TTCCAGGTCT ACCCGGACTA
    CCTGGCCCGG AAGGAGAGAA GGGAAACCCA GGTCCACTGG TTAATGGTCC TGAAGGATTC
    CCAGGTCTGC CTGGACCCCC TGGAGGTCCT GGCCCCCCGG GTCCTCCTGG CAGGACCACT
    GATCCCTGTG TCATCTATCC TGTCAGTCCT GAAGGCTCTC CTGAGGATGT GACCTCCGGT
    CTACCCGGAG TACCCGGGCA GCCTGGCCCC AGAGGACCTC CTGGAGACCG AGGTTTCAAA
    GGCTTCGGAG GTGACCCTGG TGACTGTGAC TGTGAGGGAG GGGGCTCCTC AGGGGCAACT
    GGGTTACCTG GACCTCCTGG ACTGAATGGC AGCCCTGGTG CCACTGGAAG GAAGGGTGAA
    CAGGGTGACA CTGGATCATC GGGGTTCAGC GGGCCTTCTG GTTCTGATGG GCGGCCTGGT
    AGCAGGGGCT TCTTCGGTTT GAAAGGAGAA AAGGGGGAAC CATATTTATC ACGGGGCTCT
    TCGAGCAAGG GGGATCGTGG AGCACCCGGT CCCAGAGGAC CCATAGGTGA AACTGGAAGA
    CCTGGCAGAG ATGGGCTGCC TGGATTTGCT GGACTTCCTG GAAGTCCTGG CGACCCTGGC
    TTTGGTACAG TTGGAGAAAA GGGATTCTTA GGGTTCCCGG GAATCAAAGG TCGTCCTGGA
    GCCCCTGGAG AGTCCGGCAT TGGCTACATT GGACTGCCTG GCTTGCGAGG AGAGCCTGGA
    GACCCAGGTT TTTCTGGGTT GCCTGGGCCA CCAGGTTTAC CTGGACCAAG AGGTGAAAGC
    GCCTGTGGAC GGGTTAATGG CGCCAAAACG GGAACGGGTG ATTTGTTGCC CTGCAAAATA
    CCTGGACAAG TCGGAGACCC TGGGATTCCT GGCTTACCTG GACGCCCTGG CCTTAAGGGT
    CAGCCGGGTC GGCCGGGAGA ACCTGGACGT CCAGGACTTG ATGGTTCCAA AGGGGAGAGG
    GGAGATCCTG GCTTTGGGAA CCAGCCCGGT TCTCCAGGTT TTCCAGGACC TAGAGGGGAC
    CCAGGAATCC CAGGTGTTCC TGGGCTGCGC ATTGATGGGA CCAGGGGAAG AAATGGTGTT
    CCAGGGCTTC CTGGAGCCAA AGGACAGCCT GGAGAGATTC TTGGAGCCAT ACCTGGACTT
    CCAGGACGAG ATGGTGAACC TGGAATACGT GGGGACAAGG GCCAGCCCGG CTCTCCAGGA
    GTTCCTGGAG GTCCTGGTCT GGATGGAAAG AACGGTATTC CTGGACTGAG AGGAGAACGT
    GGAATTGATG GTCTGCCGGG TGTTCCTGGT TTTCCTGGAC CTCCGGGTGA GGTTATCGGC
    CCTACTCTTG GACTGCCTGG ACCTCCTGGC AGCAAAGGAC AGATTGGATC ACCGGGCTGC
    CCAGGTCTTC CTGGGGAGAA AGGAGACACA GGGGATCCAG GTTTCCCAGG ACCTGCAGGA
    ATGAAAGGAA CACCTGGGTT TCCTGGTACT CCTGGAACAC CAGGTCCACG GGGACCGCCT
    GGACCTCCAG GACCTGGAAC TGTTGACGGA TTACCTGGAA TTCCTGGAGT AAAAGGGGAA
    AGAGGTTTTG TTGGACTCAC TGGTTTTCCT GGACAGTCTG GACGGCCCGG AACACCAGGA
    TTTCCAGGAG GGAAAGGTCT GCCGGGGGAG CCTGGAAGAA ATGGATCACC TGGCAGACTT
    GGACCTCAAG GCAACAGAGG TGACAGAGGG CTTCCAGGTC TTCCAGGCCC CCCCAGTATT
    TACACACCAG TTGTGGCGAA GGGAGAACTG GGAGATCCTG GAGCCAGAGG GTTTGATGGT
    TTTCCTGGAC CCAGAGGTGA CAAGGGTCTG CCTGGCCTGC CCGGTCGACA AGGTCGCCCT
    GGTCTTCCTG GTCTACCTGA GCAAAGTAAA GGGCAACCCG GGCAGCCGGG CCTACCTGGA
    CTGCCGGGAT CTCCAGGCTT ACCGGGACCA AAGGGAGAAG TTGGAATCAA TGGGTTCCCT
    GGCATGCCAG GACCAAGAGG AGATGATGGA ATACCTGGTG CACCCGGAGG TCCTGGAGAA
    CCAGGTCGTC CAGGTGGAAA AGGTCAACCT GGGGAAACCT TTGGTTTTCC TGGCTCACCT
    GGACAGAAAG GTCAACCGGG AGATCCAGGC AGCCCAGGAC GTCAAGGCTT TGATGGAGCC
    CCCGGTGATA ATGGCTTGTC TGGAAACCCT GGTTTCCCTG GAATAAAAGG TGGTCCTGGT
    GAACCAGGAC GGCCTGGATT ACCTGGATCT GTTGGTTTTC CAGGCCCTAA AGGGCTGTCA
    ACATTCCCTG GAAGAAAAGG AGCAGATGGG CCCCCAGGAC AGCCTGGATT TCCAGGAGGT
    CCAGGAGCCA GGGGTGTGCC TGGACCTCCT GGCCTGGATG GACTCAATGG CCTCGCTGGA
    TCCAAAGGCC TTCCTGGAAC CCCAGGTATT ACTTTAGGAG GTCGCCCTGG TGCTCCTGGT
    ATCCCAGGAT TGAAGGGAGA GAGGGGAGAT TCTTATCCTG GACAACGAGG CTTTGATGGA
    GTAAAAGGAC AAAAAGGAGA GCCTGGTCTT CCTGGAACTT CAGGAATTCC TGGTCAACCT
    GGACTTCCTG GAGAGTCAGC GTTAGCAGGC ATTGCTGGAC CTGTGGGGGA CCCAGGACTT
    CCTGGATTGG ATGGAAACTA TGGTTTTCAA GGTCCACCTG GTCCTCCTGG TCCCCCGGGT
    TCAGGAACAA TACAAGGAGA AAGAGGTGAA CCAGGGCTGC CAGGCTTCCC GGGATCTCCT
    GGAAGAAAGG GAGAACCCGG TTTCAATGGA GGCCCTGGAT ACCCAGGTAG CGCCGGTGTC
    AAAGGATCTC CTGGTGAAAG TGGTTTTAAT GGACGTCCAG GTCTTAAAGG TGTGCCTGGA
    GATCCTGGCT TCTATGGAAT CAAGGGATCT AAAGGAACAC CAGGCCAAAA AGGACGGAAG
    GGTCCTCCTG GAGTTGTGCT GCCTTCCCCT ATTGTGGAAT CACAGCCTTC TGGCGACATG
    GGTCTTCCTG GTGAAAGGGG ACCCTTGGGT TACCGTGGAG ACTCTGGTTG GAGTGGCATT
    CCAGGGCGTC CTGGTCCAAA AGGACGACCC GGAGCCCCAG GTAACCTTGG AAGGACTGGA
    CCGTCTGGTC TGCCTGGTCC GTTAGGTGAT CCCGGCTCCC CTGGTTTACC TGGAACAAGT
    GGAAGACAAG GCCCCCCTGG AAACATTGGC CTCCCTGGCA TTCCTGGCAG CTCCAGCCGC
    AGTATCAGCA TTGGCTACAC TCTGGTAAAG CACAGCCAGA GCGAGCAGGT CCCCATGTGT
    CCTCAGGGCA TGCCTTCATT GTGGAATGGG TACAGCTTGC TGTATGTGGA GGGACAGGAA
    AAAGCACACA ACCAAGACCT GGGTCAGCCT GGCTCGTGCC TCCCCAGGTT CAGCACCATG
    CCCTTCCTGT ACTGCTCCCC CAATGAGGTT TGCTTCTTTG CTAGTCGCAA TGACAAATCC
    TACTGGCTCT CCACATCTGC ACCCATTCCC ATGATGCCCG TGTCTGCGGG GCAGATCCAG
    CCTTACATCA GCAGATGTTC AGTATGTGAG GCACCATCTC AGGCTGTGGC GGTCCACAGT
    CAGGACATGA CCATCCCCAT GTGCCCCCCT GGGTGGAGAA GTCTTTGGAT TGGATATTCC
    TTCTTGATGC ACACGGCGGC TGGTGCAGAA GGCGGCGGTC AGTCCCTGAT GTCTCCCGGC
    TCCTGCTTGG AGGATTTCCG CGCCACTCCA TTCATTGAGT GCAATGGAGC CAAAGGCACA
    TGCCACTACT TTGCCAACAA ATACAGCTTC TGGCTGACCA CCGTGGATCC CAAGCGGGAG
    TTTGTGTCTT CACCAAACCA GGAGACCCTG AAGGCGGGCC AGGAGCGCTC CAGAGTCAGT
    CGCTGTCAGG TCTGCAGCAA ACTGCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa74010
    Clone ID Related Accession (Same CDS sequence) XM_020711737.1
    Accession Version XM_020711737.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5133bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1491408000000
    Organism Oryzias latipes(Japanese medaka)
    Product collagen alpha-6(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGTGACGTG GGTTTGCCCG GTGAGCCCGG CCAGCCTCTC
    ATCAATGGGA TTGTGGAGTT TGTGGGTTAT CCCAAAGGAG ACAAAGGAGA GCAGGGGGAG
    CCAGGTCCAA AAGGATCAAT TGGATTTCAA GGATTACCTG GACTGCCTGG AGCCTTTGGT
    TATCCAGGTG AAATTGGAGA GAAAGGCATC CCTGGATATC CTGGAGGAAG AGGTTCTCCA
    GGTTTTAATG GACCTCAGGG TGCTCCAGGA TTACCGGGAC AGAGAGGAGA CACAGGTTAC
    TCCGGCAGGC CAGGTTACGA CGGGCCAAAG GGCGACCCAG GAGACCGAGG TCCTCGAGGT
    CCTCCTATTT ATGTCAGTAA TATTGGCAGA ACTGCTCAAG GTCCTTCAGG AGATCCTGGT
    TACAGCGGTG CTCCTGGCGC TCCCGGAGAC CCAGGACCCA CAGGTTTCCC AGGACTTCCA
    GGTGAACCTG GACGTCTTCT GCCTGGTTCA GAAACATCAG GACTTCCAGG TCTACCCGGA
    CTACCTGGCC CGGAAGGAGA GAAGGGAAAC CCAGGTCCAC TGGTTAATGG TCCTGAAGGA
    TTCCCAGGTC TGCCTGGACC CCCTGGAGGT CCTGGCCCCC CGGGTCCTCC TGGCAGGACC
    ACTGATCCCT GTGTCATCTA TCCTGTCAGT CCTGAAGGCT CTCCTGAGGA TGTGACCTCC
    GGTCTACCCG GAGTACCCGG GCAGCCTGGC CCCAGAGGAC CTCCTGGAGA CCGAGGTTTC
    AAAGGCTTCG GAGGTGACCC TGGTGACTGT GACTGTGAGG GAGGGGGCTC CTCAGGGGCA
    ACTGGGTTAC CTGGACCTCC TGGACTGAAT GGCAGCCCTG GTGCCACTGG AAGGAAGGGT
    GAACAGGGTG ACACTGGATC ATCGGGGTTC AGCGGGCCTT CTGGTTCTGA TGGGCGGCCT
    GGTAGCAGGG GCTTCTTCGG TTTGAAAGGA GAAAAGGGGG AACCATATTT ATCACGGGGC
    TCTTCGAGCA AGGGGGATCG TGGAGCACCC GGTCCCAGAG GACCCATAGG TGAAACTGGA
    AGACCTGGCA GAGATGGGCT GCCTGGATTT GCTGGACTTC CTGGAAGTCC TGGCGACCCT
    GGCTTTGGTA CAGTTGGAGA AAAGGGATTC TTAGGGTTCC CGGGAATCAA AGGTCGTCCT
    GGAGCCCCTG GAGAGTCCGG CATTGGCTAC ATTGGACTGC CTGGCTTGCG AGGAGAGCCT
    GGAGACCCAG GTTTTTCTGG GTTGCCTGGG CCACCAGGTT TACCTGGACC AAGAGGTGAA
    AGCGCCTGTG GACGGGTTAA TGGCGCCAAA ACGGGAACGG GTGATTTGTT GCCCTGCAAA
    ATACCTGGAC AAGTCGGAGA CCCTGGGATT CCTGGCTTAC CTGGACGCCC TGGCCTTAAG
    GGTCAGCCGG GTCGGCCGGG AGAACCTGGA CGTCCAGGAC TTGATGGTTC CAAAGGGGAG
    AGGGGAGATC CTGGCTTTGG GAACCAGCCC GGTTCTCCAG GTTTTCCAGG ACCTAGAGGG
    GACCCAGGAA TCCCAGGTGT TCCTGGGCTG CGCATTGATG GGACCAGGGG AAGAAATGGT
    GTTCCAGGGC TTCCTGGAGC CAAAGGACAG CCTGGAGAGA TTCTTGGAGC CATACCTGGA
    CTTCCAGGAC GAGATGGTGA ACCTGGAATA CGTGGGGACA AGGGCCAGCC CGGCTCTCCA
    GGAGTTCCTG GAGGTCCTGG TCTGGATGGA AAGAACGGTA TTCCTGGACT GAGAGGAGAA
    CGTGGAATTG ATGGTCTGCC GGGTGTTCCT GGTTTTCCTG GACCTCCGGG TGAGGTTATC
    GGCCCTACTC TTGGACTGCC TGGACCTCCT GGCAGCAAAG GACAGATTGG ATCACCGGGC
    TGCCCAGGTC TTCCTGGGGA GAAAGGAGAC ACAGGGGATC CAGGTTTCCC AGGACCTGCA
    GGAATGAAAG GAACACCTGG GTTTCCTGGT ACTCCTGGAA CACCAGGTCC ACGGGGACCG
    CCTGGACCTC CAGGACCTGG AACTGTTGAC GGATTACCTG GAATTCCTGG AGTAAAAGGG
    GAAAGAGGTT TTGTTGGACT CACTGGTTTT CCTGGACAGT CTGGACGGCC CGGAACACCA
    GGATTTCCAG GAGGGAAAGG TCTGCCGGGG GAGCCTGGAA GAAATGGATC ACCTGGCAGA
    CTTGGACCTC AAGGCAACAG AGGTGACAGA GGGCTTCCAG GTCTTCCAGG CCCCCCCAGT
    ATTTACACAC CAGTTGTGGC GAAGGGAGAA CTGGGAGATC CTGGAGCCAG AGGGTTTGAT
    GGTTTTCCTG GACCCAGAGG TGACAAGGGT CTGCCTGGCC TGCCCGGTCG ACAAGGTCGC
    CCTGGTCTTC CTGGTCTACC TGAGCAAAGT AAAGGGCAAC CCGGGCAGCC GGGCCTACCT
    GGACTGCCGG GATCTCCAGG CTTACCGGGA CCAAAGGGAG AAGTTGGAAT CAATGGGTTC
    CCTGGCATGC CAGGACCAAG AGGAGATGAT GGAATACCTG GTGCACCCGG AGGTCCTGGA
    GAACCAGGTC GTCCAGGTGG AAAAGGTCAA CCTGGGGAAA CCTTTGGTTT TCCTGGCTCA
    CCTGGACAGA AAGGTCAACC GGGAGATCCA GGCAGCCCAG GACGTCAAGG CTTTGATGGA
    GCCCCCGGTG ATAATGGCTT GTCTGGAAAC CCTGGTTTCC CTGGAATAAA AGGTGGTCCT
    GGTGAACCAG GACGGCCTGG ATTACCTGGA TCTGTTGGTT TTCCAGGCCC TAAAGGGCTG
    TCAACATTCC CTGGAAGAAA AGGAGCAGAT GGGCCCCCAG GACAGCCTGG ATTTCCAGGA
    GGTCCAGGAG CCAGGGGTGT GCCTGGACCT CCTGGCCTGG ATGGACTCAA TGGCCTCGCT
    GGATCCAAAG GCCTTCCTGG AACCCCAGGT ATTACTTTAG GAGGTCGCCC TGGTGCTCCT
    GGTATCCCAG GATTGAAGGG AGAGAGGGGA GATTCTTATC CTGGACAACG AGGCTTTGAT
    GGAGTAAAAG GACAAAAAGG AGAGCCTGGT CTTCCTGGAA CTTCAGGAAT TCCTGGTCAA
    CCTGGACTTC CTGGAGAGTC AGCGTTAGCA GGCATTGCTG GACCTGTGGG GGACCCAGGA
    CTTCCTGGAT TGGATGGAAA CTATGGTTTT CAAGGTCCAC CTGGTCCTCC TGGTCCCCCG
    GGTTCAGGAA CAATACAAGG AGAAAGAGGT GAACCAGGGC TGCCAGGCTT CCCGGGATCT
    CCTGGAAGAA AGGGAGAACC CGGTTTCAAT GGAGGCCCTG GATACCCAGG TAGCGCCGGT
    GTCAAAGGAT CTCCTGGTGA AAGTGGTTTT AATGGACGTC CAGGTCTTAA AGGTGTGCCT
    GGAGATCCTG GCTTCTATGG AATCAAGGGA TCTAAAGGAA CACCAGGCCA AAAAGGACGG
    AAGGGTCCTC CTGGAGTTGT GCTGCCTTCC CCTATTGTGG AATCACAGCC TTCTGGCGAC
    ATGGGTCTTC CTGGTGAAAG GGGACCCTTG GGTTACCGTG GAGACTCTGG TTGGAGTGGC
    ATTCCAGGGC GTCCTGGTCC AAAAGGACGA CCCGGAGCCC CAGGTAACCT TGGAAGGACT
    GGACCGTCTG GTCTGCCTGG TCCGTTAGGT GATCCCGGCT CCCCTGGTTT ACCTGGAACA
    AGTGGAAGAC AAGGCCCCCC TGGAAACATT GGCCTCCCTG GCATTCCTGG CAGCTCCAGC
    CGCAGTATCA GCATTGGCTA CACTCTGGTA AAGCACAGCC AGAGCGAGCA GGTCCCCATG
    TGTCCTCAGG GCATGCCTTC ATTGTGGAAT GGGTACAGCT TGCTGTATGT GGAGGGACAG
    GAAAAAGCAC ACAACCAAGA CCTGGGTCAG CCTGGCTCGT GCCTCCCCAG GTTCAGCACC
    ATGCCCTTCC TGTACTGCTC CCCCAATGAG GTTTGCTTCT TTGCTAGTCG CAATGACAAA
    TCCTACTGGC TCTCCACATC TGCACCCATT CCCATGATGC CCGTGTCTGC GGGGCAGATC
    CAGCCTTACA TCAGCAGATG TTCAGTATGT GAGGCACCAT CTCAGGCTGT GGCGGTCCAC
    AGTCAGGACA TGACCATCCC CATGTGCCCC CCTGGGTGGA GAAGTCTTTG GATTGGATAT
    TCCTTCTTGA TGCACACGGC GGCTGGTGCA GAAGGCGGCG GTCAGTCCCT GATGTCTCCC
    GGCTCCTGCT TGGAGGATTT CCGCGCCACT CCATTCATTG AGTGCAATGG AGCCAAAGGC
    ACATGCCACT ACTTTGCCAA CAAATACAGC TTCTGGCTGA CCACCGTGGA TCCCAAGCGG
    GAGTTTGTGT CTTCACCAAA CCAGGAGACC CTGAAGGCGG GCCAGGAGCG CTCCAGAGTC
    AGTCGCTGTC AGGTCTGCAG CAAACTGCTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020567396.1
    CDS489..5621
    Translation

    Target ORF information:

    RefSeq Version XM_020711737.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_020711737.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGTGACGTG GGTTTGCCCG GTGAGCCCGG CCAGCCTCTC
    ATCAATGGGA TTGTGGAGTT TGTGGGTTAT CCCAAAGGAG ACAAAGGAGA GCAGGGGGAG
    CCAGGTCCAA AAGGATCAAT TGGATTTCAA GGATTACCTG GACTGCCTGG AGCCTTTGGT
    TATCCAGGTG AAATTGGAGA GAAAGGCATC CCTGGATATC CTGGAGGAAG AGGTTCTCCA
    GGTTTTAATG GACCTCAGGG TGCTCCAGGA TTACCGGGAC AGAGAGGAGA CACAGGTTAC
    TCCGGCAGGC CAGGTTACGA CGGGCCAAAG GGCGACCCAG GAGACCGAGG TCCTCGAGGT
    CCTCCTATTT ATGTCAGTAA TATTGGCAGA ACTGCTCAAG GTCCTTCAGG AGATCCTGGT
    TACAGCGGTG CTCCTGGCGC TCCCGGAGAC CCAGGACCCA CAGGTTTCCC AGGACTTCCA
    GGTGAACCTG GACGTCTTCT GCCTGGTTCA GAAACATCAG GACTTCCAGG TCTACCCGGA
    CTACCTGGCC CGGAAGGAGA GAAGGGAAAC CCAGGTCCAC TGGTTAATGG TCCTGAAGGA
    TTCCCAGGTC TGCCTGGACC CCCTGGAGGT CCTGGCCCCC CGGGTCCTCC TGGCAGGACC
    ACTGATCCCT GTGTCATCTA TCCTGTCAGT CCTGAAGGCT CTCCTGAGGA TGTGACCTCC
    GGTCTACCCG GAGTACCCGG GCAGCCTGGC CCCAGAGGAC CTCCTGGAGA CCGAGGTTTC
    AAAGGCTTCG GAGGTGACCC TGGTGACTGT GACTGTGAGG GAGGGGGCTC CTCAGGGGCA
    ACTGGGTTAC CTGGACCTCC TGGACTGAAT GGCAGCCCTG GTGCCACTGG AAGGAAGGGT
    GAACAGGGTG ACACTGGATC ATCGGGGTTC AGCGGGCCTT CTGGTTCTGA TGGGCGGCCT
    GGTAGCAGGG GCTTCTTCGG TTTGAAAGGA GAAAAGGGGG AACCATATTT ATCACGGGGC
    TCTTCGAGCA AGGGGGATCG TGGAGCACCC GGTCCCAGAG GACCCATAGG TGAAACTGGA
    AGACCTGGCA GAGATGGGCT GCCTGGATTT GCTGGACTTC CTGGAAGTCC TGGCGACCCT
    GGCTTTGGTA CAGTTGGAGA AAAGGGATTC TTAGGGTTCC CGGGAATCAA AGGTCGTCCT
    GGAGCCCCTG GAGAGTCCGG CATTGGCTAC ATTGGACTGC CTGGCTTGCG AGGAGAGCCT
    GGAGACCCAG GTTTTTCTGG GTTGCCTGGG CCACCAGGTT TACCTGGACC AAGAGGTGAA
    AGCGCCTGTG GACGGGTTAA TGGCGCCAAA ACGGGAACGG GTGATTTGTT GCCCTGCAAA
    ATACCTGGAC AAGTCGGAGA CCCTGGGATT CCTGGCTTAC CTGGACGCCC TGGCCTTAAG
    GGTCAGCCGG GTCGGCCGGG AGAACCTGGA CGTCCAGGAC TTGATGGTTC CAAAGGGGAG
    AGGGGAGATC CTGGCTTTGG GAACCAGCCC GGTTCTCCAG GTTTTCCAGG ACCTAGAGGG
    GACCCAGGAA TCCCAGGTGT TCCTGGGCTG CGCATTGATG GGACCAGGGG AAGAAATGGT
    GTTCCAGGGC TTCCTGGAGC CAAAGGACAG CCTGGAGAGA TTCTTGGAGC CATACCTGGA
    CTTCCAGGAC GAGATGGTGA ACCTGGAATA CGTGGGGACA AGGGCCAGCC CGGCTCTCCA
    GGAGTTCCTG GAGGTCCTGG TCTGGATGGA AAGAACGGTA TTCCTGGACT GAGAGGAGAA
    CGTGGAATTG ATGGTCTGCC GGGTGTTCCT GGTTTTCCTG GACCTCCGGG TGAGGTTATC
    GGCCCTACTC TTGGACTGCC TGGACCTCCT GGCAGCAAAG GACAGATTGG ATCACCGGGC
    TGCCCAGGTC TTCCTGGGGA GAAAGGAGAC ACAGGGGATC CAGGTTTCCC AGGACCTGCA
    GGAATGAAAG GAACACCTGG GTTTCCTGGT ACTCCTGGAA CACCAGGTCC ACGGGGACCG
    CCTGGACCTC CAGGACCTGG AACTGTTGAC GGATTACCTG GAATTCCTGG AGTAAAAGGG
    GAAAGAGGTT TTGTTGGACT CACTGGTTTT CCTGGACAGT CTGGACGGCC CGGAACACCA
    GGATTTCCAG GAGGGAAAGG TCTGCCGGGG GAGCCTGGAA GAAATGGATC ACCTGGCAGA
    CTTGGACCTC AAGGCAACAG AGGTGACAGA GGGCTTCCAG GTCTTCCAGG CCCCCCCAGT
    ATTTACACAC CAGTTGTGGC GAAGGGAGAA CTGGGAGATC CTGGAGCCAG AGGGTTTGAT
    GGTTTTCCTG GACCCAGAGG TGACAAGGGT CTGCCTGGCC TGCCCGGTCG ACAAGGTCGC
    CCTGGTCTTC CTGGTCTACC TGAGCAAAGT AAAGGGCAAC CCGGGCAGCC GGGCCTACCT
    GGACTGCCGG GATCTCCAGG CTTACCGGGA CCAAAGGGAG AAGTTGGAAT CAATGGGTTC
    CCTGGCATGC CAGGACCAAG AGGAGATGAT GGAATACCTG GTGCACCCGG AGGTCCTGGA
    GAACCAGGTC GTCCAGGTGG AAAAGGTCAA CCTGGGGAAA CCTTTGGTTT TCCTGGCTCA
    CCTGGACAGA AAGGTCAACC GGGAGATCCA GGCAGCCCAG GACGTCAAGG CTTTGATGGA
    GCCCCCGGTG ATAATGGCTT GTCTGGAAAC CCTGGTTTCC CTGGAATAAA AGGTGGTCCT
    GGTGAACCAG GACGGCCTGG ATTACCTGGA TCTGTTGGTT TTCCAGGCCC TAAAGGGCTG
    TCAACATTCC CTGGAAGAAA AGGAGCAGAT GGGCCCCCAG GACAGCCTGG ATTTCCAGGA
    GGTCCAGGAG CCAGGGGTGT GCCTGGACCT CCTGGCCTGG ATGGACTCAA TGGCCTCGCT
    GGATCCAAAG GCCTTCCTGG AACCCCAGGT ATTACTTTAG GAGGTCGCCC TGGTGCTCCT
    GGTATCCCAG GATTGAAGGG AGAGAGGGGA GATTCTTATC CTGGACAACG AGGCTTTGAT
    GGAGTAAAAG GACAAAAAGG AGAGCCTGGT CTTCCTGGAA CTTCAGGAAT TCCTGGTCAA
    CCTGGACTTC CTGGAGAGTC AGCGTTAGCA GGCATTGCTG GACCTGTGGG GGACCCAGGA
    CTTCCTGGAT TGGATGGAAA CTATGGTTTT CAAGGTCCAC CTGGTCCTCC TGGTCCCCCG
    GGTTCAGGAA CAATACAAGG AGAAAGAGGT GAACCAGGGC TGCCAGGCTT CCCGGGATCT
    CCTGGAAGAA AGGGAGAACC CGGTTTCAAT GGAGGCCCTG GATACCCAGG TAGCGCCGGT
    GTCAAAGGAT CTCCTGGTGA AAGTGGTTTT AATGGACGTC CAGGTCTTAA AGGTGTGCCT
    GGAGATCCTG GCTTCTATGG AATCAAGGGA TCTAAAGGAA CACCAGGCCA AAAAGGACGG
    AAGGGTCCTC CTGGAGTTGT GCTGCCTTCC CCTATTGTGG AATCACAGCC TTCTGGCGAC
    ATGGGTCTTC CTGGTGAAAG GGGACCCTTG GGTTACCGTG GAGACTCTGG TTGGAGTGGC
    ATTCCAGGGC GTCCTGGTCC AAAAGGACGA CCCGGAGCCC CAGGTAACCT TGGAAGGACT
    GGACCGTCTG GTCTGCCTGG TCCGTTAGGT GATCCCGGCT CCCCTGGTTT ACCTGGAACA
    AGTGGAAGAC AAGGCCCCCC TGGAAACATT GGCCTCCCTG GCATTCCTGG CAGCTCCAGC
    CGCAGTATCA GCATTGGCTA CACTCTGGTA AAGCACAGCC AGAGCGAGCA GGTCCCCATG
    TGTCCTCAGG GCATGCCTTC ATTGTGGAAT GGGTACAGCT TGCTGTATGT GGAGGGACAG
    GAAAAAGCAC ACAACCAAGA CCTGGGTCAG CCTGGCTCGT GCCTCCCCAG GTTCAGCACC
    ATGCCCTTCC TGTACTGCTC CCCCAATGAG GTTTGCTTCT TTGCTAGTCG CAATGACAAA
    TCCTACTGGC TCTCCACATC TGCACCCATT CCCATGATGC CCGTGTCTGC GGGGCAGATC
    CAGCCTTACA TCAGCAGATG TTCAGTATGT GAGGCACCAT CTCAGGCTGT GGCGGTCCAC
    AGTCAGGACA TGACCATCCC CATGTGCCCC CCTGGGTGGA GAAGTCTTTG GATTGGATAT
    TCCTTCTTGA TGCACACGGC GGCTGGTGCA GAAGGCGGCG GTCAGTCCCT GATGTCTCCC
    GGCTCCTGCT TGGAGGATTT CCGCGCCACT CCATTCATTG AGTGCAATGG AGCCAAAGGC
    ACATGCCACT ACTTTGCCAA CAAATACAGC TTCTGGCTGA CCACCGTGGA TCCCAAGCGG
    GAGTTTGTGT CTTCACCAAA CCAGGAGACC CTGAAGGCGG GCCAGGAGCG CTCCAGAGTC
    AGTCGCTGTC AGGTCTGCAG CAAACTGCTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa74011
    Clone ID Related Accession (Same CDS sequence) XM_020711738.1
    Accession Version XM_020711738.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5124bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1491408000000
    Organism Oryzias latipes(Japanese medaka)
    Product collagen alpha-6(IV) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGTGACGTG GGTTTGCCCG GTGAGCCCGG CCAGCCTCTC
    ATCAATGGGA TTGTGGAGTT TGTGGGTTAT CCCAAAGGAG ACAAAGGAGA GCAGGGGGAG
    CCAGGTCCAA AAGGATCAAT TGGATTTCAA GGATTACCTG GACTGCCTGG AGCCTTTGGT
    TATCCAGGTG AAATTGGAGA GAAAGGCATC CCTGGATATC CTGGAGGAAG AGGTTCTCCA
    GGTTTTAATG GACCTCAGGG TGCTCCAGGA TTACCGGGAC AGAGAGGAGA CACAGGTTAC
    TCCGGCAGGC CAGGTTACGA CGGGCCAAAG GGCGACCCAG GAGACCGAGG TCCTCGAGGT
    CCTCCTATTT ATGTCAGTAA TATTGGCAGA ACTGCTCAAG GTCCTTCAGG AGATCCTGGT
    TACAGCGGTG CTCCTGGCGC TCCCGGAGAC CCAGGACCCA CAGGTTTCCC AGGACTTCCA
    GGTGAACCTG GACGTCTTCT GCCTGGTTCA GAAACATCAG GACTTCCAGG TCTACCCGGA
    CTACCTGGCC CGGAAGGAGA GAAGGGAAAC CCAGGTCCAC TGGTTAATGG TCCTGAAGGA
    TTCCCAGGTC TGCCTGGACC CCCTGGAGGT CCTGGCCCCC CGGGTCCTCC TGGCAGGACC
    ACTGATCCCT GTGTCATCTA TCCTGTCAGT CCTGAAGGCT CTCCTGAGGA TGTGACCTCC
    GGTCTACCCG GAGTACCCGG GCAGCCTGGC CCCAGAGGAC CTCCTGGAGA CCGAGGTTTC
    AAAGGCTTCG GAGGTGACCC TGGTGACTGT GACTGTGAGG GAGGGGGCTC CTCAGGGGCA
    ACTGGGTTAC CTGGACCTCC TGGACTGAAT GGCAGCCCTG GTGCCACTGG AAGGAAGGGT
    GAACAGGGTG ACACTGGATC ATCGGGGTTC AGCGGGCCTT CTGGTTCTGA TGGGCGGCCT
    GGTAGCAGGG GCTTCTTCGG TTTGAAAGGA GAAAAGGGGG AACCATATTT ATCACGGGGC
    TCTTCGAGCA AGGGGGATCG TGGAGCACCC GGTCCCAGAG GACCCATAGG TGAAACTGGA
    AGACCTGGCA GAGATGGGCT GCCTGGATTT GCTGGACTTC CTGGAAGTCC TGGCGACCCT
    GGCTTTGGTA CAGTTGGAGA AAAGGGATTC TTAGGGTTCC CGGGAATCAA AGGTCGTCCT
    GGAGCCCCTG GAGAGTCCGG CATTGGCTAC ATTGGACTGC CTGGCTTGCG AGGAGAGCCT
    GGAGACCCAG GTTTTTCTGG GTTGCCTGGG CCACCAGGTT TACCTGGACC AAGAGGTGAA
    AGCGCCTGTG GACGGGTTAA TGGCGCCAAA ACGGGAACGG GTGATTTGTT GCCCTGCAAA
    ATACCTGGAC AAGTCGGAGA CCCTGGGATT CCTGGCTTAC CTGGACGCCC TGGCCTTAAG
    GGTCAGCCGG GTCGGCCGGG AGAACCTGGA CGTCCAGGAC TTGATGGTTC CAAAGGGGAG
    AGGGGAGATC CTGGCTTTGG GAACCAGCCC GGTTCTCCAG GTTTTCCAGG ACCTAGAGGG
    GACCCAGGAA TCCCAGGTGT TCCTGGGCTG CGCATTGATG GGACCAGGGG AAGAAATGGT
    GTTCCAGGGC TTCCTGGAGC CAAAGGACAG CCTGGAGAGA TTCTTGGAGC CATACCTGGA
    CTTCCAGGAC GAGATGGTGA ACCTGGAATA CGTGGGGACA AGGGCCAGCC CGGCTCTCCA
    GGAGTTCCTG GAGGTCCTGG TCTGGATGGA AAGAACGGTA TTCCTGGACT GAGAGGAGAA
    CGTGGAATTG ATGGTCTGCC GGGTGTTCCT GGTTTTCCTG GACCTCCGGG TGAGGTTATC
    GGCCCTACTC TTGGACTGCC TGGACCTCCT GGCAGCAAAG GACAGATTGG ATCACCGGGT
    CTTCCTGGGG AGAAAGGAGA CACAGGGGAT CCAGGTTTCC CAGGACCTGC AGGAATGAAA
    GGAACACCTG GGTTTCCTGG TACTCCTGGA ACACCAGGTC CACGGGGACC GCCTGGACCT
    CCAGGACCTG GAACTGTTGA CGGATTACCT GGAATTCCTG GAGTAAAAGG GGAAAGAGGT
    TTTGTTGGAC TCACTGGTTT TCCTGGACAG TCTGGACGGC CCGGAACACC AGGATTTCCA
    GGAGGGAAAG GTCTGCCGGG GGAGCCTGGA AGAAATGGAT CACCTGGCAG ACTTGGACCT
    CAAGGCAACA GAGGTGACAG AGGGCTTCCA GGTCTTCCAG GCCCCCCCAG TATTTACACA
    CCAGTTGTGG CGAAGGGAGA ACTGGGAGAT CCTGGAGCCA GAGGGTTTGA TGGTTTTCCT
    GGACCCAGAG GTGACAAGGG TCTGCCTGGC CTGCCCGGTC GACAAGGTCG CCCTGGTCTT
    CCTGGTCTAC CTGAGCAAAG TAAAGGGCAA CCCGGGCAGC CGGGCCTACC TGGACTGCCG
    GGATCTCCAG GCTTACCGGG ACCAAAGGGA GAAGTTGGAA TCAATGGGTT CCCTGGCATG
    CCAGGACCAA GAGGAGATGA TGGAATACCT GGTGCACCCG GAGGTCCTGG AGAACCAGGT
    CGTCCAGGTG GAAAAGGTCA ACCTGGGGAA ACCTTTGGTT TTCCTGGCTC ACCTGGACAG
    AAAGGTCAAC CGGGAGATCC AGGCAGCCCA GGACGTCAAG GCTTTGATGG AGCCCCCGGT
    GATAATGGCT TGTCTGGAAA CCCTGGTTTC CCTGGAATAA AAGGTGGTCC TGGTGAACCA
    GGACGGCCTG GATTACCTGG ATCTGTTGGT TTTCCAGGCC CTAAAGGGCT GTCAACATTC
    CCTGGAAGAA AAGGAGCAGA TGGGCCCCCA GGACAGCCTG GATTTCCAGG AGGTCCAGGA
    GCCAGGGGTG TGCCTGGACC TCCTGGCCTG GATGGACTCA ATGGCCTCGC TGGATCCAAA
    GGCCTTCCTG GAACCCCAGG TATTACTTTA GGAGGTCGCC CTGGTGCTCC TGGTATCCCA
    GGATTGAAGG GAGAGAGGGG AGATTCTTAT CCTGGACAAC GAGGCTTTGA TGGAGTAAAA
    GGACAAAAAG GAGAGCCTGG TCTTCCTGGA ACTTCAGGAA TTCCTGGTCA ACCTGGACTT
    CCTGGAGAGT CAGCGTTAGC AGGCATTGCT GGACCTGTGG GGGACCCAGG ACTTCCTGGA
    TTGGATGGAA ACTATGGTTT TCAAGGTCCA CCTGGTCCTC CTGGTCCCCC GGGTTCAGGA
    ACAATACAAG GAGAAAGAGG TGAACCAGGG CTGCCAGGCT TCCCGGGATC TCCTGGAAGA
    AAGGGAGAAC CCGGTTTCAA TGGAGGCCCT GGATACCCAG GTAGCGCCGG TGTCAAAGGA
    TCTCCTGGTG AAAGTGGTTT TAATGGACGT CCAGGTCTTA AAGGTGTGCC TGGAGATCCT
    GGCTTCTATG GAATCAAGGG ATCTAAAGGA ACACCAGGCC AAAAAGGACG GAAGGGTCCT
    CCTGGAGTTG TGCTGCCTTC CCCTATTGTG GAATCACAGC CTTCTGGCGA CATGGGTCTT
    CCTGGTGAAA GGGGACCCTT GGGTTACCGT GGAGACTCTG GTTGGAGTGG CATTCCAGGG
    CGTCCTGGTC CAAAAGGACG ACCCGGAGCC CCAGGTAACC TTGGAAGGAC TGGACCGTCT
    GGTCTGCCTG GTCCGTTAGG TGATCCCGGC TCCCCTGGTT TACCTGGAAC AAGTGGAAGA
    CAAGGCCCCC CTGGAAACAT TGGCCTCCCT GGCATTCCTG GCAGCTCCAG CCGCAGTATC
    AGCATTGGCT ACACTCTGGT AAAGCACAGC CAGAGCGAGC AGGTCCCCAT GTGTCCTCAG
    GGCATGCCTT CATTGTGGAA TGGGTACAGC TTGCTGTATG TGGAGGGACA GGAAAAAGCA
    CACAACCAAG ACCTGGGTCA GCCTGGCTCG TGCCTCCCCA GGTTCAGCAC CATGCCCTTC
    CTGTACTGCT CCCCCAATGA GGTTTGCTTC TTTGCTAGTC GCAATGACAA ATCCTACTGG
    CTCTCCACAT CTGCACCCAT TCCCATGATG CCCGTGTCTG CGGGGCAGAT CCAGCCTTAC
    ATCAGCAGAT GTTCAGTATG TGAGGCACCA TCTCAGGCTG TGGCGGTCCA CAGTCAGGAC
    ATGACCATCC CCATGTGCCC CCCTGGGTGG AGAAGTCTTT GGATTGGATA TTCCTTCTTG
    ATGCACACGG CGGCTGGTGC AGAAGGCGGC GGTCAGTCCC TGATGTCTCC CGGCTCCTGC
    TTGGAGGATT TCCGCGCCAC TCCATTCATT GAGTGCAATG GAGCCAAAGG CACATGCCAC
    TACTTTGCCA ACAAATACAG CTTCTGGCTG ACCACCGTGG ATCCCAAGCG GGAGTTTGTG
    TCTTCACCAA ACCAGGAGAC CCTGAAGGCG GGCCAGGAGC GCTCCAGAGT CAGTCGCTGT
    CAGGTCTGCA GCAAACTGCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020567397.1
    CDS489..5612
    Translation

    Target ORF information:

    RefSeq Version XM_020711738.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_020711738.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGTGACGTG GGTTTGCCCG GTGAGCCCGG CCAGCCTCTC
    ATCAATGGGA TTGTGGAGTT TGTGGGTTAT CCCAAAGGAG ACAAAGGAGA GCAGGGGGAG
    CCAGGTCCAA AAGGATCAAT TGGATTTCAA GGATTACCTG GACTGCCTGG AGCCTTTGGT
    TATCCAGGTG AAATTGGAGA GAAAGGCATC CCTGGATATC CTGGAGGAAG AGGTTCTCCA
    GGTTTTAATG GACCTCAGGG TGCTCCAGGA TTACCGGGAC AGAGAGGAGA CACAGGTTAC
    TCCGGCAGGC CAGGTTACGA CGGGCCAAAG GGCGACCCAG GAGACCGAGG TCCTCGAGGT
    CCTCCTATTT ATGTCAGTAA TATTGGCAGA ACTGCTCAAG GTCCTTCAGG AGATCCTGGT
    TACAGCGGTG CTCCTGGCGC TCCCGGAGAC CCAGGACCCA CAGGTTTCCC AGGACTTCCA
    GGTGAACCTG GACGTCTTCT GCCTGGTTCA GAAACATCAG GACTTCCAGG TCTACCCGGA
    CTACCTGGCC CGGAAGGAGA GAAGGGAAAC CCAGGTCCAC TGGTTAATGG TCCTGAAGGA
    TTCCCAGGTC TGCCTGGACC CCCTGGAGGT CCTGGCCCCC CGGGTCCTCC TGGCAGGACC
    ACTGATCCCT GTGTCATCTA TCCTGTCAGT CCTGAAGGCT CTCCTGAGGA TGTGACCTCC
    GGTCTACCCG GAGTACCCGG GCAGCCTGGC CCCAGAGGAC CTCCTGGAGA CCGAGGTTTC
    AAAGGCTTCG GAGGTGACCC TGGTGACTGT GACTGTGAGG GAGGGGGCTC CTCAGGGGCA
    ACTGGGTTAC CTGGACCTCC TGGACTGAAT GGCAGCCCTG GTGCCACTGG AAGGAAGGGT
    GAACAGGGTG ACACTGGATC ATCGGGGTTC AGCGGGCCTT CTGGTTCTGA TGGGCGGCCT
    GGTAGCAGGG GCTTCTTCGG TTTGAAAGGA GAAAAGGGGG AACCATATTT ATCACGGGGC
    TCTTCGAGCA AGGGGGATCG TGGAGCACCC GGTCCCAGAG GACCCATAGG TGAAACTGGA
    AGACCTGGCA GAGATGGGCT GCCTGGATTT GCTGGACTTC CTGGAAGTCC TGGCGACCCT
    GGCTTTGGTA CAGTTGGAGA AAAGGGATTC TTAGGGTTCC CGGGAATCAA AGGTCGTCCT
    GGAGCCCCTG GAGAGTCCGG CATTGGCTAC ATTGGACTGC CTGGCTTGCG AGGAGAGCCT
    GGAGACCCAG GTTTTTCTGG GTTGCCTGGG CCACCAGGTT TACCTGGACC AAGAGGTGAA
    AGCGCCTGTG GACGGGTTAA TGGCGCCAAA ACGGGAACGG GTGATTTGTT GCCCTGCAAA
    ATACCTGGAC AAGTCGGAGA CCCTGGGATT CCTGGCTTAC CTGGACGCCC TGGCCTTAAG
    GGTCAGCCGG GTCGGCCGGG AGAACCTGGA CGTCCAGGAC TTGATGGTTC CAAAGGGGAG
    AGGGGAGATC CTGGCTTTGG GAACCAGCCC GGTTCTCCAG GTTTTCCAGG ACCTAGAGGG
    GACCCAGGAA TCCCAGGTGT TCCTGGGCTG CGCATTGATG GGACCAGGGG AAGAAATGGT
    GTTCCAGGGC TTCCTGGAGC CAAAGGACAG CCTGGAGAGA TTCTTGGAGC CATACCTGGA
    CTTCCAGGAC GAGATGGTGA ACCTGGAATA CGTGGGGACA AGGGCCAGCC CGGCTCTCCA
    GGAGTTCCTG GAGGTCCTGG TCTGGATGGA AAGAACGGTA TTCCTGGACT GAGAGGAGAA
    CGTGGAATTG ATGGTCTGCC GGGTGTTCCT GGTTTTCCTG GACCTCCGGG TGAGGTTATC
    GGCCCTACTC TTGGACTGCC TGGACCTCCT GGCAGCAAAG GACAGATTGG ATCACCGGGT
    CTTCCTGGGG AGAAAGGAGA CACAGGGGAT CCAGGTTTCC CAGGACCTGC AGGAATGAAA
    GGAACACCTG GGTTTCCTGG TACTCCTGGA ACACCAGGTC CACGGGGACC GCCTGGACCT
    CCAGGACCTG GAACTGTTGA CGGATTACCT GGAATTCCTG GAGTAAAAGG GGAAAGAGGT
    TTTGTTGGAC TCACTGGTTT TCCTGGACAG TCTGGACGGC CCGGAACACC AGGATTTCCA
    GGAGGGAAAG GTCTGCCGGG GGAGCCTGGA AGAAATGGAT CACCTGGCAG ACTTGGACCT
    CAAGGCAACA GAGGTGACAG AGGGCTTCCA GGTCTTCCAG GCCCCCCCAG TATTTACACA
    CCAGTTGTGG CGAAGGGAGA ACTGGGAGAT CCTGGAGCCA GAGGGTTTGA TGGTTTTCCT
    GGACCCAGAG GTGACAAGGG TCTGCCTGGC CTGCCCGGTC GACAAGGTCG CCCTGGTCTT
    CCTGGTCTAC CTGAGCAAAG TAAAGGGCAA CCCGGGCAGC CGGGCCTACC TGGACTGCCG
    GGATCTCCAG GCTTACCGGG ACCAAAGGGA GAAGTTGGAA TCAATGGGTT CCCTGGCATG
    CCAGGACCAA GAGGAGATGA TGGAATACCT GGTGCACCCG GAGGTCCTGG AGAACCAGGT
    CGTCCAGGTG GAAAAGGTCA ACCTGGGGAA ACCTTTGGTT TTCCTGGCTC ACCTGGACAG
    AAAGGTCAAC CGGGAGATCC AGGCAGCCCA GGACGTCAAG GCTTTGATGG AGCCCCCGGT
    GATAATGGCT TGTCTGGAAA CCCTGGTTTC CCTGGAATAA AAGGTGGTCC TGGTGAACCA
    GGACGGCCTG GATTACCTGG ATCTGTTGGT TTTCCAGGCC CTAAAGGGCT GTCAACATTC
    CCTGGAAGAA AAGGAGCAGA TGGGCCCCCA GGACAGCCTG GATTTCCAGG AGGTCCAGGA
    GCCAGGGGTG TGCCTGGACC TCCTGGCCTG GATGGACTCA ATGGCCTCGC TGGATCCAAA
    GGCCTTCCTG GAACCCCAGG TATTACTTTA GGAGGTCGCC CTGGTGCTCC TGGTATCCCA
    GGATTGAAGG GAGAGAGGGG AGATTCTTAT CCTGGACAAC GAGGCTTTGA TGGAGTAAAA
    GGACAAAAAG GAGAGCCTGG TCTTCCTGGA ACTTCAGGAA TTCCTGGTCA ACCTGGACTT
    CCTGGAGAGT CAGCGTTAGC AGGCATTGCT GGACCTGTGG GGGACCCAGG ACTTCCTGGA
    TTGGATGGAA ACTATGGTTT TCAAGGTCCA CCTGGTCCTC CTGGTCCCCC GGGTTCAGGA
    ACAATACAAG GAGAAAGAGG TGAACCAGGG CTGCCAGGCT TCCCGGGATC TCCTGGAAGA
    AAGGGAGAAC CCGGTTTCAA TGGAGGCCCT GGATACCCAG GTAGCGCCGG TGTCAAAGGA
    TCTCCTGGTG AAAGTGGTTT TAATGGACGT CCAGGTCTTA AAGGTGTGCC TGGAGATCCT
    GGCTTCTATG GAATCAAGGG ATCTAAAGGA ACACCAGGCC AAAAAGGACG GAAGGGTCCT
    CCTGGAGTTG TGCTGCCTTC CCCTATTGTG GAATCACAGC CTTCTGGCGA CATGGGTCTT
    CCTGGTGAAA GGGGACCCTT GGGTTACCGT GGAGACTCTG GTTGGAGTGG CATTCCAGGG
    CGTCCTGGTC CAAAAGGACG ACCCGGAGCC CCAGGTAACC TTGGAAGGAC TGGACCGTCT
    GGTCTGCCTG GTCCGTTAGG TGATCCCGGC TCCCCTGGTT TACCTGGAAC AAGTGGAAGA
    CAAGGCCCCC CTGGAAACAT TGGCCTCCCT GGCATTCCTG GCAGCTCCAG CCGCAGTATC
    AGCATTGGCT ACACTCTGGT AAAGCACAGC CAGAGCGAGC AGGTCCCCAT GTGTCCTCAG
    GGCATGCCTT CATTGTGGAA TGGGTACAGC TTGCTGTATG TGGAGGGACA GGAAAAAGCA
    CACAACCAAG ACCTGGGTCA GCCTGGCTCG TGCCTCCCCA GGTTCAGCAC CATGCCCTTC
    CTGTACTGCT CCCCCAATGA GGTTTGCTTC TTTGCTAGTC GCAATGACAA ATCCTACTGG
    CTCTCCACAT CTGCACCCAT TCCCATGATG CCCGTGTCTG CGGGGCAGAT CCAGCCTTAC
    ATCAGCAGAT GTTCAGTATG TGAGGCACCA TCTCAGGCTG TGGCGGTCCA CAGTCAGGAC
    ATGACCATCC CCATGTGCCC CCCTGGGTGG AGAAGTCTTT GGATTGGATA TTCCTTCTTG
    ATGCACACGG CGGCTGGTGC AGAAGGCGGC GGTCAGTCCC TGATGTCTCC CGGCTCCTGC
    TTGGAGGATT TCCGCGCCAC TCCATTCATT GAGTGCAATG GAGCCAAAGG CACATGCCAC
    TACTTTGCCA ACAAATACAG CTTCTGGCTG ACCACCGTGG ATCCCAAGCG GGAGTTTGTG
    TCTTCACCAA ACCAGGAGAC CCTGAAGGCG GGCCAGGAGC GCTCCAGAGT CAGTCGCTGT
    CAGGTCTGCA GCAAACTGCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa74012
    Clone ID Related Accession (Same CDS sequence) XM_020711739.2 , XM_020711739.1
    Accession Version XM_020711739.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1518537600000
    Organism Oryzias latipes(Japanese medaka)
    Product collagen alpha-6(IV) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_020711739.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGTGACGTG GGTTTGCCCG GTGAGCCCGG CCAGCCTCTC
    ATCAATGGGA TTGTGGAGTT TGTGGGTTAT CCCAAAGGAG ACAAAGGAGA GCAGGGGGAG
    CCAGGTCCAA AAGGATCAAT TGGATTTCAA GGATTACCTG GACTGCCTGG AGCCTTTGGT
    TATCCAGGTG AAATTGGAGA GAAAGGCATC CCTGGATATC CTGGAGGAAG AGGTTCTCCA
    GGTTTTAATG GACCTCAGGG TGCTCCAGGA TTACCGGGAC AGAGAGGAGA CACAGGTTAC
    TCCGGCAGGC CAGGTTACGA CGGGCCAAAG GGCGACCCAG GAGACCGAGG TCCTCGAGGT
    CCTCCTATTT ATGTCAGTAA TATTGGCAGA ACTGCTCAAG GTCCTTCAGG AGATCCTGGT
    TACAGCGGTG CTCCTGGCGC TCCCGGAGAC CCAGGACCCA CAGGTTTCCC AGGACTTCCA
    GGTGAACCTG GACGTCTTCT GCCTGGTTCA GAAACATCAG GACTTCCAGG TCTACCCGGA
    CTACCTGGCC CGGAAGGAGA GAAGGGAAAC CCAGGTCCAC TGGTTAATGG TCCTGAAGGA
    TTCCCAGGTC TGCCTGGACC CCCTGGAGGT CCTGGCCCCC CGGGTCCTCC TGGCAGGACC
    ACTGATCCCT GTGTCATCTA TCCTGTCAGT CCTGAAGGCT CTCCTGAGGA TGTGACCTCC
    GGTCTACCCG GAGTACCCGG GCAGCCTGGC CCCAGAGGAC CTCCTGGAGA CCGAGGTTTC
    AAAGGCTTCG GAGGTGACCC TGGTGACTGT GACTGTGAGG GAGGGGGCTC CTCAGGGGCA
    ACTGGGTTAC CTGGACCTCC TGGACTGAAT GGCAGCCCTG GTGCCACTGG AAGGAAGGGT
    GAACAGGGTG ACACTGGATC ATCGGGGTTC AGCGGGCCTT CTGGTTCTGA TGGGCGGCCT
    GGTAGCAGGG GCTTCTTCGG TTTGAAAGGA GAAAAGGGGG AACCATATTT ATCACGGGGC
    TCTTCGAGCA AGGGGGATCG TGGAGCACCC GGTCCCAGAG GACCCATAGG TGAAACTGGA
    AGACCTGGCA GAGATGGGCT GCCTGGATTT GCTGGACTTC CTGGAAGTCC TGGCGACCCT
    GGCTTTGGTA CAGTTGGAGA AAAGGGATTC TTAGGGTTCC CGGGAATCAA AGGTCGTCCT
    GGAGCCCCTG GAGAGTCCGG CATTGGCTAC ATTGGACTGC CTGGCTTGCG AGGAGAGCCT
    GGAGACCCAG GTTTTTCTGG GTTGCCTGGG CCACCAGGTT TACCTGGACC AAGAGGTGAT
    TTGTTGCCCT GCAAAATACC TGGACAAGTC GGAGACCCTG GGATTCCTGG CTTACCTGGA
    CGCCCTGGCC TTAAGGGTCA GCCGGGTCGG CCGGGAGAAC CTGGACGTCC AGGACTTGAT
    GGTTCCAAAG GGGAGAGGGG AGATCCTGGC TTTGGGAACC AGCCCGGTTC TCCAGGTTTT
    CCAGGACCTA GAGGGGACCC AGGAATCCCA GGTGTTCCTG GGCTGCGCAT TGATGGGACC
    AGGGGAAGAA ATGGTGTTCC AGGGCTTCCT GGAGCCAAAG GACAGCCTGG AGAGATTCTT
    GGAGCCATAC CTGGACTTCC AGGACGAGAT GGTGAACCTG GAATACGTGG GGACAAGGGC
    CAGCCCGGCT CTCCAGGAGT TCCTGGAGGT CCTGGTCTGG ATGGAAAGAA CGGTATTCCT
    GGACTGAGAG GAGAACGTGG AATTGATGGT CTGCCGGGTG TTCCTGGTTT TCCTGGACCT
    CCGGGTGAGG TTATCGGCCC TACTCTTGGA CTGCCTGGAC CTCCTGGCAG CAAAGGACAG
    ATTGGATCAC CGGGCTGCCC AGGTCTTCCT GGGGAGAAAG GAGACACAGG GGATCCAGGT
    TTCCCAGGAC CTGCAGGAAT GAAAGGAACA CCTGGGTTTC CTGGTACTCC TGGAACACCA
    GGTCCACGGG GACCGCCTGG ACCTCCAGGA CCTGGAACTG TTGACGGATT ACCTGGAATT
    CCTGGAGTAA AAGGGGAAAG AGGTTTTGTT GGACTCACTG GTTTTCCTGG ACAGTCTGGA
    CGGCCCGGAA CACCAGGATT TCCAGGAGGG AAAGGTCTGC CGGGGGAGCC TGGAAGAAAT
    GGATCACCTG GCAGACTTGG ACCTCAAGGC AACAGAGGTG ACAGAGGGCT TCCAGGTCTT
    CCAGGCCCCC CCAGTATTTA CACACCAGTT GTGGCGAAGG GAGAACTGGG AGATCCTGGA
    GCCAGAGGGT TTGATGGTTT TCCTGGACCC AGAGGTGACA AGGGTCTGCC TGGCCTGCCC
    GGTCGACAAG GTCGCCCTGG TCTTCCTGGT CTACCTGAGC AAAGTAAAGG GCAACCCGGG
    CAGCCGGGCC TACCTGGACT GCCGGGATCT CCAGGCTTAC CGGGACCAAA GGGAGAAGTT
    GGAATCAATG GGTTCCCTGG CATGCCAGGA CCAAGAGGAG ATGATGGAAT ACCTGGTGCA
    CCCGGAGGTC CTGGAGAACC AGGTCGTCCA GGTGGAAAAG GTCAACCTGG GGAAACCTTT
    GGTTTTCCTG GCTCACCTGG ACAGAAAGGT CAACCGGGAG ATCCAGGCAG CCCAGGACGT
    CAAGGCTTTG ATGGAGCCCC CGGTGATAAT GGCTTGTCTG GAAACCCTGG TTTCCCTGGA
    ATAAAAGGTG GTCCTGGTGA ACCAGGACGG CCTGGATTAC CTGGATCTGT TGGTTTTCCA
    GGCCCTAAAG GGCTGTCAAC ATTCCCTGGA AGAAAAGGAG CAGATGGGCC CCCAGGACAG
    CCTGGATTTC CAGGAGGTCC AGGAGCCAGG GGTGTGCCTG GACCTCCTGG CCTGGATGGA
    CTCAATGGCC TCGCTGGATC CAAAGGCCTT CCTGGAACCC CAGGTATTAC TTTAGGAGGT
    CGCCCTGGTG CTCCTGGTAT CCCAGGATTG AAGGGAGAGA GGGGAGATTC TTATCCTGGA
    CAACGAGGCT TTGATGGAGT AAAAGGACAA AAAGGAGAGC CTGGTCTTCC TGGAACTTCA
    GGAATTCCTG GTCAACCTGG ACTTCCTGGA GAGTCAGCGT TAGCAGGCAT TGCTGGACCT
    GTGGGGGACC CAGGACTTCC TGGATTGGAT GGAAACTATG GTTTTCAAGG TCCACCTGGT
    CCTCCTGGTC CCCCGGGTTC AGGAACAATA CAAGGAGAAA GAGGTGAACC AGGGCTGCCA
    GGCTTCCCGG GATCTCCTGG AAGAAAGGGA GAACCCGGTT TCAATGGAGG CCCTGGATAC
    CCAGGTAGCG CCGGTGTCAA AGGATCTCCT GGTGAAAGTG GTTTTAATGG ACGTCCAGGT
    CTTAAAGGTG TGCCTGGAGA TCCTGGCTTC TATGGAATCA AGGGATCTAA AGGAACACCA
    GGCCAAAAAG GACGGAAGGG TCCTCCTGGA GTTGTGCTGC CTTCCCCTAT TGTGGAATCA
    CAGCCTTCTG GCGACATGGG TCTTCCTGGT GAAAGGGGAC CCTTGGGTTA CCGTGGAGAC
    TCTGGTTGGA GTGGCATTCC AGGGCGTCCT GGTCCAAAAG GACGACCCGG AGCCCCAGGT
    AACCTTGGAA GGACTGGACC GTCTGGTCTG CCTGGTCCGT TAGGTGATCC CGGCTCCCCT
    GGTTTACCTG GAACAAGTGG AAGACAAGGC CCCCCTGGAA ACATTGGCCT CCCTGGCATT
    CCTGGCAGCT CCAGCCGCAG TATCAGCATT GGCTACACTC TGGTAAAGCA CAGCCAGAGC
    GAGCAGGTCC CCATGTGTCC TCAGGGCATG CCTTCATTGT GGAATGGGTA CAGCTTGCTG
    TATGTGGAGG GACAGGAAAA AGCACACAAC CAAGACCTGG GTCAGCCTGG CTCGTGCCTC
    CCCAGGTTCA GCACCATGCC CTTCCTGTAC TGCTCCCCCA ATGAGGTTTG CTTCTTTGCT
    AGTCGCAATG ACAAATCCTA CTGGCTCTCC ACATCTGCAC CCATTCCCAT GATGCCCGTG
    TCTGCGGGGC AGATCCAGCC TTACATCAGC AGATGTTCAG TATGTGAGGC ACCATCTCAG
    GCTGTGGCGG TCCACAGTCA GGACATGACC ATCCCCATGT GCCCCCCTGG GTGGAGAAGT
    CTTTGGATTG GATATTCCTT CTTGATGCAC ACGGCGGCTG GTGCAGAAGG CGGCGGTCAG
    TCCCTGATGT CTCCCGGCTC CTGCTTGGAG GATTTCCGCG CCACTCCATT CATTGAGTGC
    AATGGAGCCA AAGGCACATG CCACTACTTT GCCAACAAAT ACAGCTTCTG GCTGACCACC
    GTGGATCCCA AGCGGGAGTT TGTGTCTTCA CCAAACCAGG AGACCCTGAA GGCGGGCCAG
    GAGCGCTCCA GAGTCAGTCG CTGTCAGGTC TGCAGCAAAC TGCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020567398.1
    CDS480..5567
    Translation

    Target ORF information:

    RefSeq Version XM_020711739.2
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_020711739.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGTGACGTG GGTTTGCCCG GTGAGCCCGG CCAGCCTCTC
    ATCAATGGGA TTGTGGAGTT TGTGGGTTAT CCCAAAGGAG ACAAAGGAGA GCAGGGGGAG
    CCAGGTCCAA AAGGATCAAT TGGATTTCAA GGATTACCTG GACTGCCTGG AGCCTTTGGT
    TATCCAGGTG AAATTGGAGA GAAAGGCATC CCTGGATATC CTGGAGGAAG AGGTTCTCCA
    GGTTTTAATG GACCTCAGGG TGCTCCAGGA TTACCGGGAC AGAGAGGAGA CACAGGTTAC
    TCCGGCAGGC CAGGTTACGA CGGGCCAAAG GGCGACCCAG GAGACCGAGG TCCTCGAGGT
    CCTCCTATTT ATGTCAGTAA TATTGGCAGA ACTGCTCAAG GTCCTTCAGG AGATCCTGGT
    TACAGCGGTG CTCCTGGCGC TCCCGGAGAC CCAGGACCCA CAGGTTTCCC AGGACTTCCA
    GGTGAACCTG GACGTCTTCT GCCTGGTTCA GAAACATCAG GACTTCCAGG TCTACCCGGA
    CTACCTGGCC CGGAAGGAGA GAAGGGAAAC CCAGGTCCAC TGGTTAATGG TCCTGAAGGA
    TTCCCAGGTC TGCCTGGACC CCCTGGAGGT CCTGGCCCCC CGGGTCCTCC TGGCAGGACC
    ACTGATCCCT GTGTCATCTA TCCTGTCAGT CCTGAAGGCT CTCCTGAGGA TGTGACCTCC
    GGTCTACCCG GAGTACCCGG GCAGCCTGGC CCCAGAGGAC CTCCTGGAGA CCGAGGTTTC
    AAAGGCTTCG GAGGTGACCC TGGTGACTGT GACTGTGAGG GAGGGGGCTC CTCAGGGGCA
    ACTGGGTTAC CTGGACCTCC TGGACTGAAT GGCAGCCCTG GTGCCACTGG AAGGAAGGGT
    GAACAGGGTG ACACTGGATC ATCGGGGTTC AGCGGGCCTT CTGGTTCTGA TGGGCGGCCT
    GGTAGCAGGG GCTTCTTCGG TTTGAAAGGA GAAAAGGGGG AACCATATTT ATCACGGGGC
    TCTTCGAGCA AGGGGGATCG TGGAGCACCC GGTCCCAGAG GACCCATAGG TGAAACTGGA
    AGACCTGGCA GAGATGGGCT GCCTGGATTT GCTGGACTTC CTGGAAGTCC TGGCGACCCT
    GGCTTTGGTA CAGTTGGAGA AAAGGGATTC TTAGGGTTCC CGGGAATCAA AGGTCGTCCT
    GGAGCCCCTG GAGAGTCCGG CATTGGCTAC ATTGGACTGC CTGGCTTGCG AGGAGAGCCT
    GGAGACCCAG GTTTTTCTGG GTTGCCTGGG CCACCAGGTT TACCTGGACC AAGAGGTGAT
    TTGTTGCCCT GCAAAATACC TGGACAAGTC GGAGACCCTG GGATTCCTGG CTTACCTGGA
    CGCCCTGGCC TTAAGGGTCA GCCGGGTCGG CCGGGAGAAC CTGGACGTCC AGGACTTGAT
    GGTTCCAAAG GGGAGAGGGG AGATCCTGGC TTTGGGAACC AGCCCGGTTC TCCAGGTTTT
    CCAGGACCTA GAGGGGACCC AGGAATCCCA GGTGTTCCTG GGCTGCGCAT TGATGGGACC
    AGGGGAAGAA ATGGTGTTCC AGGGCTTCCT GGAGCCAAAG GACAGCCTGG AGAGATTCTT
    GGAGCCATAC CTGGACTTCC AGGACGAGAT GGTGAACCTG GAATACGTGG GGACAAGGGC
    CAGCCCGGCT CTCCAGGAGT TCCTGGAGGT CCTGGTCTGG ATGGAAAGAA CGGTATTCCT
    GGACTGAGAG GAGAACGTGG AATTGATGGT CTGCCGGGTG TTCCTGGTTT TCCTGGACCT
    CCGGGTGAGG TTATCGGCCC TACTCTTGGA CTGCCTGGAC CTCCTGGCAG CAAAGGACAG
    ATTGGATCAC CGGGCTGCCC AGGTCTTCCT GGGGAGAAAG GAGACACAGG GGATCCAGGT
    TTCCCAGGAC CTGCAGGAAT GAAAGGAACA CCTGGGTTTC CTGGTACTCC TGGAACACCA
    GGTCCACGGG GACCGCCTGG ACCTCCAGGA CCTGGAACTG TTGACGGATT ACCTGGAATT
    CCTGGAGTAA AAGGGGAAAG AGGTTTTGTT GGACTCACTG GTTTTCCTGG ACAGTCTGGA
    CGGCCCGGAA CACCAGGATT TCCAGGAGGG AAAGGTCTGC CGGGGGAGCC TGGAAGAAAT
    GGATCACCTG GCAGACTTGG ACCTCAAGGC AACAGAGGTG ACAGAGGGCT TCCAGGTCTT
    CCAGGCCCCC CCAGTATTTA CACACCAGTT GTGGCGAAGG GAGAACTGGG AGATCCTGGA
    GCCAGAGGGT TTGATGGTTT TCCTGGACCC AGAGGTGACA AGGGTCTGCC TGGCCTGCCC
    GGTCGACAAG GTCGCCCTGG TCTTCCTGGT CTACCTGAGC AAAGTAAAGG GCAACCCGGG
    CAGCCGGGCC TACCTGGACT GCCGGGATCT CCAGGCTTAC CGGGACCAAA GGGAGAAGTT
    GGAATCAATG GGTTCCCTGG CATGCCAGGA CCAAGAGGAG ATGATGGAAT ACCTGGTGCA
    CCCGGAGGTC CTGGAGAACC AGGTCGTCCA GGTGGAAAAG GTCAACCTGG GGAAACCTTT
    GGTTTTCCTG GCTCACCTGG ACAGAAAGGT CAACCGGGAG ATCCAGGCAG CCCAGGACGT
    CAAGGCTTTG ATGGAGCCCC CGGTGATAAT GGCTTGTCTG GAAACCCTGG TTTCCCTGGA
    ATAAAAGGTG GTCCTGGTGA ACCAGGACGG CCTGGATTAC CTGGATCTGT TGGTTTTCCA
    GGCCCTAAAG GGCTGTCAAC ATTCCCTGGA AGAAAAGGAG CAGATGGGCC CCCAGGACAG
    CCTGGATTTC CAGGAGGTCC AGGAGCCAGG GGTGTGCCTG GACCTCCTGG CCTGGATGGA
    CTCAATGGCC TCGCTGGATC CAAAGGCCTT CCTGGAACCC CAGGTATTAC TTTAGGAGGT
    CGCCCTGGTG CTCCTGGTAT CCCAGGATTG AAGGGAGAGA GGGGAGATTC TTATCCTGGA
    CAACGAGGCT TTGATGGAGT AAAAGGACAA AAAGGAGAGC CTGGTCTTCC TGGAACTTCA
    GGAATTCCTG GTCAACCTGG ACTTCCTGGA GAGTCAGCGT TAGCAGGCAT TGCTGGACCT
    GTGGGGGACC CAGGACTTCC TGGATTGGAT GGAAACTATG GTTTTCAAGG TCCACCTGGT
    CCTCCTGGTC CCCCGGGTTC AGGAACAATA CAAGGAGAAA GAGGTGAACC AGGGCTGCCA
    GGCTTCCCGG GATCTCCTGG AAGAAAGGGA GAACCCGGTT TCAATGGAGG CCCTGGATAC
    CCAGGTAGCG CCGGTGTCAA AGGATCTCCT GGTGAAAGTG GTTTTAATGG ACGTCCAGGT
    CTTAAAGGTG TGCCTGGAGA TCCTGGCTTC TATGGAATCA AGGGATCTAA AGGAACACCA
    GGCCAAAAAG GACGGAAGGG TCCTCCTGGA GTTGTGCTGC CTTCCCCTAT TGTGGAATCA
    CAGCCTTCTG GCGACATGGG TCTTCCTGGT GAAAGGGGAC CCTTGGGTTA CCGTGGAGAC
    TCTGGTTGGA GTGGCATTCC AGGGCGTCCT GGTCCAAAAG GACGACCCGG AGCCCCAGGT
    AACCTTGGAA GGACTGGACC GTCTGGTCTG CCTGGTCCGT TAGGTGATCC CGGCTCCCCT
    GGTTTACCTG GAACAAGTGG AAGACAAGGC CCCCCTGGAA ACATTGGCCT CCCTGGCATT
    CCTGGCAGCT CCAGCCGCAG TATCAGCATT GGCTACACTC TGGTAAAGCA CAGCCAGAGC
    GAGCAGGTCC CCATGTGTCC TCAGGGCATG CCTTCATTGT GGAATGGGTA CAGCTTGCTG
    TATGTGGAGG GACAGGAAAA AGCACACAAC CAAGACCTGG GTCAGCCTGG CTCGTGCCTC
    CCCAGGTTCA GCACCATGCC CTTCCTGTAC TGCTCCCCCA ATGAGGTTTG CTTCTTTGCT
    AGTCGCAATG ACAAATCCTA CTGGCTCTCC ACATCTGCAC CCATTCCCAT GATGCCCGTG
    TCTGCGGGGC AGATCCAGCC TTACATCAGC AGATGTTCAG TATGTGAGGC ACCATCTCAG
    GCTGTGGCGG TCCACAGTCA GGACATGACC ATCCCCATGT GCCCCCCTGG GTGGAGAAGT
    CTTTGGATTG GATATTCCTT CTTGATGCAC ACGGCGGCTG GTGCAGAAGG CGGCGGTCAG
    TCCCTGATGT CTCCCGGCTC CTGCTTGGAG GATTTCCGCG CCACTCCATT CATTGAGTGC
    AATGGAGCCA AAGGCACATG CCACTACTTT GCCAACAAAT ACAGCTTCTG GCTGACCACC
    GTGGATCCCA AGCGGGAGTT TGTGTCTTCA CCAAACCAGG AGACCCTGAA GGCGGGCCAG
    GAGCGCTCCA GAGTCAGTCG CTGTCAGGTC TGCAGCAAAC TGCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa74012
    Clone ID Related Accession (Same CDS sequence) XM_020711739.2 , XM_020711739.1
    Accession Version XM_020711739.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1491408000000
    Organism Oryzias latipes(Japanese medaka)
    Product collagen alpha-6(IV) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGTGACGTG GGTTTGCCCG GTGAGCCCGG CCAGCCTCTC
    ATCAATGGGA TTGTGGAGTT TGTGGGTTAT CCCAAAGGAG ACAAAGGAGA GCAGGGGGAG
    CCAGGTCCAA AAGGATCAAT TGGATTTCAA GGATTACCTG GACTGCCTGG AGCCTTTGGT
    TATCCAGGTG AAATTGGAGA GAAAGGCATC CCTGGATATC CTGGAGGAAG AGGTTCTCCA
    GGTTTTAATG GACCTCAGGG TGCTCCAGGA TTACCGGGAC AGAGAGGAGA CACAGGTTAC
    TCCGGCAGGC CAGGTTACGA CGGGCCAAAG GGCGACCCAG GAGACCGAGG TCCTCGAGGT
    CCTCCTATTT ATGTCAGTAA TATTGGCAGA ACTGCTCAAG GTCCTTCAGG AGATCCTGGT
    TACAGCGGTG CTCCTGGCGC TCCCGGAGAC CCAGGACCCA CAGGTTTCCC AGGACTTCCA
    GGTGAACCTG GACGTCTTCT GCCTGGTTCA GAAACATCAG GACTTCCAGG TCTACCCGGA
    CTACCTGGCC CGGAAGGAGA GAAGGGAAAC CCAGGTCCAC TGGTTAATGG TCCTGAAGGA
    TTCCCAGGTC TGCCTGGACC CCCTGGAGGT CCTGGCCCCC CGGGTCCTCC TGGCAGGACC
    ACTGATCCCT GTGTCATCTA TCCTGTCAGT CCTGAAGGCT CTCCTGAGGA TGTGACCTCC
    GGTCTACCCG GAGTACCCGG GCAGCCTGGC CCCAGAGGAC CTCCTGGAGA CCGAGGTTTC
    AAAGGCTTCG GAGGTGACCC TGGTGACTGT GACTGTGAGG GAGGGGGCTC CTCAGGGGCA
    ACTGGGTTAC CTGGACCTCC TGGACTGAAT GGCAGCCCTG GTGCCACTGG AAGGAAGGGT
    GAACAGGGTG ACACTGGATC ATCGGGGTTC AGCGGGCCTT CTGGTTCTGA TGGGCGGCCT
    GGTAGCAGGG GCTTCTTCGG TTTGAAAGGA GAAAAGGGGG AACCATATTT ATCACGGGGC
    TCTTCGAGCA AGGGGGATCG TGGAGCACCC GGTCCCAGAG GACCCATAGG TGAAACTGGA
    AGACCTGGCA GAGATGGGCT GCCTGGATTT GCTGGACTTC CTGGAAGTCC TGGCGACCCT
    GGCTTTGGTA CAGTTGGAGA AAAGGGATTC TTAGGGTTCC CGGGAATCAA AGGTCGTCCT
    GGAGCCCCTG GAGAGTCCGG CATTGGCTAC ATTGGACTGC CTGGCTTGCG AGGAGAGCCT
    GGAGACCCAG GTTTTTCTGG GTTGCCTGGG CCACCAGGTT TACCTGGACC AAGAGGTGAT
    TTGTTGCCCT GCAAAATACC TGGACAAGTC GGAGACCCTG GGATTCCTGG CTTACCTGGA
    CGCCCTGGCC TTAAGGGTCA GCCGGGTCGG CCGGGAGAAC CTGGACGTCC AGGACTTGAT
    GGTTCCAAAG GGGAGAGGGG AGATCCTGGC TTTGGGAACC AGCCCGGTTC TCCAGGTTTT
    CCAGGACCTA GAGGGGACCC AGGAATCCCA GGTGTTCCTG GGCTGCGCAT TGATGGGACC
    AGGGGAAGAA ATGGTGTTCC AGGGCTTCCT GGAGCCAAAG GACAGCCTGG AGAGATTCTT
    GGAGCCATAC CTGGACTTCC AGGACGAGAT GGTGAACCTG GAATACGTGG GGACAAGGGC
    CAGCCCGGCT CTCCAGGAGT TCCTGGAGGT CCTGGTCTGG ATGGAAAGAA CGGTATTCCT
    GGACTGAGAG GAGAACGTGG AATTGATGGT CTGCCGGGTG TTCCTGGTTT TCCTGGACCT
    CCGGGTGAGG TTATCGGCCC TACTCTTGGA CTGCCTGGAC CTCCTGGCAG CAAAGGACAG
    ATTGGATCAC CGGGCTGCCC AGGTCTTCCT GGGGAGAAAG GAGACACAGG GGATCCAGGT
    TTCCCAGGAC CTGCAGGAAT GAAAGGAACA CCTGGGTTTC CTGGTACTCC TGGAACACCA
    GGTCCACGGG GACCGCCTGG ACCTCCAGGA CCTGGAACTG TTGACGGATT ACCTGGAATT
    CCTGGAGTAA AAGGGGAAAG AGGTTTTGTT GGACTCACTG GTTTTCCTGG ACAGTCTGGA
    CGGCCCGGAA CACCAGGATT TCCAGGAGGG AAAGGTCTGC CGGGGGAGCC TGGAAGAAAT
    GGATCACCTG GCAGACTTGG ACCTCAAGGC AACAGAGGTG ACAGAGGGCT TCCAGGTCTT
    CCAGGCCCCC CCAGTATTTA CACACCAGTT GTGGCGAAGG GAGAACTGGG AGATCCTGGA
    GCCAGAGGGT TTGATGGTTT TCCTGGACCC AGAGGTGACA AGGGTCTGCC TGGCCTGCCC
    GGTCGACAAG GTCGCCCTGG TCTTCCTGGT CTACCTGAGC AAAGTAAAGG GCAACCCGGG
    CAGCCGGGCC TACCTGGACT GCCGGGATCT CCAGGCTTAC CGGGACCAAA GGGAGAAGTT
    GGAATCAATG GGTTCCCTGG CATGCCAGGA CCAAGAGGAG ATGATGGAAT ACCTGGTGCA
    CCCGGAGGTC CTGGAGAACC AGGTCGTCCA GGTGGAAAAG GTCAACCTGG GGAAACCTTT
    GGTTTTCCTG GCTCACCTGG ACAGAAAGGT CAACCGGGAG ATCCAGGCAG CCCAGGACGT
    CAAGGCTTTG ATGGAGCCCC CGGTGATAAT GGCTTGTCTG GAAACCCTGG TTTCCCTGGA
    ATAAAAGGTG GTCCTGGTGA ACCAGGACGG CCTGGATTAC CTGGATCTGT TGGTTTTCCA
    GGCCCTAAAG GGCTGTCAAC ATTCCCTGGA AGAAAAGGAG CAGATGGGCC CCCAGGACAG
    CCTGGATTTC CAGGAGGTCC AGGAGCCAGG GGTGTGCCTG GACCTCCTGG CCTGGATGGA
    CTCAATGGCC TCGCTGGATC CAAAGGCCTT CCTGGAACCC CAGGTATTAC TTTAGGAGGT
    CGCCCTGGTG CTCCTGGTAT CCCAGGATTG AAGGGAGAGA GGGGAGATTC TTATCCTGGA
    CAACGAGGCT TTGATGGAGT AAAAGGACAA AAAGGAGAGC CTGGTCTTCC TGGAACTTCA
    GGAATTCCTG GTCAACCTGG ACTTCCTGGA GAGTCAGCGT TAGCAGGCAT TGCTGGACCT
    GTGGGGGACC CAGGACTTCC TGGATTGGAT GGAAACTATG GTTTTCAAGG TCCACCTGGT
    CCTCCTGGTC CCCCGGGTTC AGGAACAATA CAAGGAGAAA GAGGTGAACC AGGGCTGCCA
    GGCTTCCCGG GATCTCCTGG AAGAAAGGGA GAACCCGGTT TCAATGGAGG CCCTGGATAC
    CCAGGTAGCG CCGGTGTCAA AGGATCTCCT GGTGAAAGTG GTTTTAATGG ACGTCCAGGT
    CTTAAAGGTG TGCCTGGAGA TCCTGGCTTC TATGGAATCA AGGGATCTAA AGGAACACCA
    GGCCAAAAAG GACGGAAGGG TCCTCCTGGA GTTGTGCTGC CTTCCCCTAT TGTGGAATCA
    CAGCCTTCTG GCGACATGGG TCTTCCTGGT GAAAGGGGAC CCTTGGGTTA CCGTGGAGAC
    TCTGGTTGGA GTGGCATTCC AGGGCGTCCT GGTCCAAAAG GACGACCCGG AGCCCCAGGT
    AACCTTGGAA GGACTGGACC GTCTGGTCTG CCTGGTCCGT TAGGTGATCC CGGCTCCCCT
    GGTTTACCTG GAACAAGTGG AAGACAAGGC CCCCCTGGAA ACATTGGCCT CCCTGGCATT
    CCTGGCAGCT CCAGCCGCAG TATCAGCATT GGCTACACTC TGGTAAAGCA CAGCCAGAGC
    GAGCAGGTCC CCATGTGTCC TCAGGGCATG CCTTCATTGT GGAATGGGTA CAGCTTGCTG
    TATGTGGAGG GACAGGAAAA AGCACACAAC CAAGACCTGG GTCAGCCTGG CTCGTGCCTC
    CCCAGGTTCA GCACCATGCC CTTCCTGTAC TGCTCCCCCA ATGAGGTTTG CTTCTTTGCT
    AGTCGCAATG ACAAATCCTA CTGGCTCTCC ACATCTGCAC CCATTCCCAT GATGCCCGTG
    TCTGCGGGGC AGATCCAGCC TTACATCAGC AGATGTTCAG TATGTGAGGC ACCATCTCAG
    GCTGTGGCGG TCCACAGTCA GGACATGACC ATCCCCATGT GCCCCCCTGG GTGGAGAAGT
    CTTTGGATTG GATATTCCTT CTTGATGCAC ACGGCGGCTG GTGCAGAAGG CGGCGGTCAG
    TCCCTGATGT CTCCCGGCTC CTGCTTGGAG GATTTCCGCG CCACTCCATT CATTGAGTGC
    AATGGAGCCA AAGGCACATG CCACTACTTT GCCAACAAAT ACAGCTTCTG GCTGACCACC
    GTGGATCCCA AGCGGGAGTT TGTGTCTTCA CCAAACCAGG AGACCCTGAA GGCGGGCCAG
    GAGCGCTCCA GAGTCAGTCG CTGTCAGGTC TGCAGCAAAC TGCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020567398.1
    CDS488..5575
    Translation

    Target ORF information:

    RefSeq Version XM_020711739.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_020711739.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGTGACGTG GGTTTGCCCG GTGAGCCCGG CCAGCCTCTC
    ATCAATGGGA TTGTGGAGTT TGTGGGTTAT CCCAAAGGAG ACAAAGGAGA GCAGGGGGAG
    CCAGGTCCAA AAGGATCAAT TGGATTTCAA GGATTACCTG GACTGCCTGG AGCCTTTGGT
    TATCCAGGTG AAATTGGAGA GAAAGGCATC CCTGGATATC CTGGAGGAAG AGGTTCTCCA
    GGTTTTAATG GACCTCAGGG TGCTCCAGGA TTACCGGGAC AGAGAGGAGA CACAGGTTAC
    TCCGGCAGGC CAGGTTACGA CGGGCCAAAG GGCGACCCAG GAGACCGAGG TCCTCGAGGT
    CCTCCTATTT ATGTCAGTAA TATTGGCAGA ACTGCTCAAG GTCCTTCAGG AGATCCTGGT
    TACAGCGGTG CTCCTGGCGC TCCCGGAGAC CCAGGACCCA CAGGTTTCCC AGGACTTCCA
    GGTGAACCTG GACGTCTTCT GCCTGGTTCA GAAACATCAG GACTTCCAGG TCTACCCGGA
    CTACCTGGCC CGGAAGGAGA GAAGGGAAAC CCAGGTCCAC TGGTTAATGG TCCTGAAGGA
    TTCCCAGGTC TGCCTGGACC CCCTGGAGGT CCTGGCCCCC CGGGTCCTCC TGGCAGGACC
    ACTGATCCCT GTGTCATCTA TCCTGTCAGT CCTGAAGGCT CTCCTGAGGA TGTGACCTCC
    GGTCTACCCG GAGTACCCGG GCAGCCTGGC CCCAGAGGAC CTCCTGGAGA CCGAGGTTTC
    AAAGGCTTCG GAGGTGACCC TGGTGACTGT GACTGTGAGG GAGGGGGCTC CTCAGGGGCA
    ACTGGGTTAC CTGGACCTCC TGGACTGAAT GGCAGCCCTG GTGCCACTGG AAGGAAGGGT
    GAACAGGGTG ACACTGGATC ATCGGGGTTC AGCGGGCCTT CTGGTTCTGA TGGGCGGCCT
    GGTAGCAGGG GCTTCTTCGG TTTGAAAGGA GAAAAGGGGG AACCATATTT ATCACGGGGC
    TCTTCGAGCA AGGGGGATCG TGGAGCACCC GGTCCCAGAG GACCCATAGG TGAAACTGGA
    AGACCTGGCA GAGATGGGCT GCCTGGATTT GCTGGACTTC CTGGAAGTCC TGGCGACCCT
    GGCTTTGGTA CAGTTGGAGA AAAGGGATTC TTAGGGTTCC CGGGAATCAA AGGTCGTCCT
    GGAGCCCCTG GAGAGTCCGG CATTGGCTAC ATTGGACTGC CTGGCTTGCG AGGAGAGCCT
    GGAGACCCAG GTTTTTCTGG GTTGCCTGGG CCACCAGGTT TACCTGGACC AAGAGGTGAT
    TTGTTGCCCT GCAAAATACC TGGACAAGTC GGAGACCCTG GGATTCCTGG CTTACCTGGA
    CGCCCTGGCC TTAAGGGTCA GCCGGGTCGG CCGGGAGAAC CTGGACGTCC AGGACTTGAT
    GGTTCCAAAG GGGAGAGGGG AGATCCTGGC TTTGGGAACC AGCCCGGTTC TCCAGGTTTT
    CCAGGACCTA GAGGGGACCC AGGAATCCCA GGTGTTCCTG GGCTGCGCAT TGATGGGACC
    AGGGGAAGAA ATGGTGTTCC AGGGCTTCCT GGAGCCAAAG GACAGCCTGG AGAGATTCTT
    GGAGCCATAC CTGGACTTCC AGGACGAGAT GGTGAACCTG GAATACGTGG GGACAAGGGC
    CAGCCCGGCT CTCCAGGAGT TCCTGGAGGT CCTGGTCTGG ATGGAAAGAA CGGTATTCCT
    GGACTGAGAG GAGAACGTGG AATTGATGGT CTGCCGGGTG TTCCTGGTTT TCCTGGACCT
    CCGGGTGAGG TTATCGGCCC TACTCTTGGA CTGCCTGGAC CTCCTGGCAG CAAAGGACAG
    ATTGGATCAC CGGGCTGCCC AGGTCTTCCT GGGGAGAAAG GAGACACAGG GGATCCAGGT
    TTCCCAGGAC CTGCAGGAAT GAAAGGAACA CCTGGGTTTC CTGGTACTCC TGGAACACCA
    GGTCCACGGG GACCGCCTGG ACCTCCAGGA CCTGGAACTG TTGACGGATT ACCTGGAATT
    CCTGGAGTAA AAGGGGAAAG AGGTTTTGTT GGACTCACTG GTTTTCCTGG ACAGTCTGGA
    CGGCCCGGAA CACCAGGATT TCCAGGAGGG AAAGGTCTGC CGGGGGAGCC TGGAAGAAAT
    GGATCACCTG GCAGACTTGG ACCTCAAGGC AACAGAGGTG ACAGAGGGCT TCCAGGTCTT
    CCAGGCCCCC CCAGTATTTA CACACCAGTT GTGGCGAAGG GAGAACTGGG AGATCCTGGA
    GCCAGAGGGT TTGATGGTTT TCCTGGACCC AGAGGTGACA AGGGTCTGCC TGGCCTGCCC
    GGTCGACAAG GTCGCCCTGG TCTTCCTGGT CTACCTGAGC AAAGTAAAGG GCAACCCGGG
    CAGCCGGGCC TACCTGGACT GCCGGGATCT CCAGGCTTAC CGGGACCAAA GGGAGAAGTT
    GGAATCAATG GGTTCCCTGG CATGCCAGGA CCAAGAGGAG ATGATGGAAT ACCTGGTGCA
    CCCGGAGGTC CTGGAGAACC AGGTCGTCCA GGTGGAAAAG GTCAACCTGG GGAAACCTTT
    GGTTTTCCTG GCTCACCTGG ACAGAAAGGT CAACCGGGAG ATCCAGGCAG CCCAGGACGT
    CAAGGCTTTG ATGGAGCCCC CGGTGATAAT GGCTTGTCTG GAAACCCTGG TTTCCCTGGA
    ATAAAAGGTG GTCCTGGTGA ACCAGGACGG CCTGGATTAC CTGGATCTGT TGGTTTTCCA
    GGCCCTAAAG GGCTGTCAAC ATTCCCTGGA AGAAAAGGAG CAGATGGGCC CCCAGGACAG
    CCTGGATTTC CAGGAGGTCC AGGAGCCAGG GGTGTGCCTG GACCTCCTGG CCTGGATGGA
    CTCAATGGCC TCGCTGGATC CAAAGGCCTT CCTGGAACCC CAGGTATTAC TTTAGGAGGT
    CGCCCTGGTG CTCCTGGTAT CCCAGGATTG AAGGGAGAGA GGGGAGATTC TTATCCTGGA
    CAACGAGGCT TTGATGGAGT AAAAGGACAA AAAGGAGAGC CTGGTCTTCC TGGAACTTCA
    GGAATTCCTG GTCAACCTGG ACTTCCTGGA GAGTCAGCGT TAGCAGGCAT TGCTGGACCT
    GTGGGGGACC CAGGACTTCC TGGATTGGAT GGAAACTATG GTTTTCAAGG TCCACCTGGT
    CCTCCTGGTC CCCCGGGTTC AGGAACAATA CAAGGAGAAA GAGGTGAACC AGGGCTGCCA
    GGCTTCCCGG GATCTCCTGG AAGAAAGGGA GAACCCGGTT TCAATGGAGG CCCTGGATAC
    CCAGGTAGCG CCGGTGTCAA AGGATCTCCT GGTGAAAGTG GTTTTAATGG ACGTCCAGGT
    CTTAAAGGTG TGCCTGGAGA TCCTGGCTTC TATGGAATCA AGGGATCTAA AGGAACACCA
    GGCCAAAAAG GACGGAAGGG TCCTCCTGGA GTTGTGCTGC CTTCCCCTAT TGTGGAATCA
    CAGCCTTCTG GCGACATGGG TCTTCCTGGT GAAAGGGGAC CCTTGGGTTA CCGTGGAGAC
    TCTGGTTGGA GTGGCATTCC AGGGCGTCCT GGTCCAAAAG GACGACCCGG AGCCCCAGGT
    AACCTTGGAA GGACTGGACC GTCTGGTCTG CCTGGTCCGT TAGGTGATCC CGGCTCCCCT
    GGTTTACCTG GAACAAGTGG AAGACAAGGC CCCCCTGGAA ACATTGGCCT CCCTGGCATT
    CCTGGCAGCT CCAGCCGCAG TATCAGCATT GGCTACACTC TGGTAAAGCA CAGCCAGAGC
    GAGCAGGTCC CCATGTGTCC TCAGGGCATG CCTTCATTGT GGAATGGGTA CAGCTTGCTG
    TATGTGGAGG GACAGGAAAA AGCACACAAC CAAGACCTGG GTCAGCCTGG CTCGTGCCTC
    CCCAGGTTCA GCACCATGCC CTTCCTGTAC TGCTCCCCCA ATGAGGTTTG CTTCTTTGCT
    AGTCGCAATG ACAAATCCTA CTGGCTCTCC ACATCTGCAC CCATTCCCAT GATGCCCGTG
    TCTGCGGGGC AGATCCAGCC TTACATCAGC AGATGTTCAG TATGTGAGGC ACCATCTCAG
    GCTGTGGCGG TCCACAGTCA GGACATGACC ATCCCCATGT GCCCCCCTGG GTGGAGAAGT
    CTTTGGATTG GATATTCCTT CTTGATGCAC ACGGCGGCTG GTGCAGAAGG CGGCGGTCAG
    TCCCTGATGT CTCCCGGCTC CTGCTTGGAG GATTTCCGCG CCACTCCATT CATTGAGTGC
    AATGGAGCCA AAGGCACATG CCACTACTTT GCCAACAAAT ACAGCTTCTG GCTGACCACC
    GTGGATCCCA AGCGGGAGTT TGTGTCTTCA CCAAACCAGG AGACCCTGAA GGCGGGCCAG
    GAGCGCTCCA GAGTCAGTCG CTGTCAGGTC TGCAGCAAAC TGCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa74013
    Clone ID Related Accession (Same CDS sequence) XM_020711740.1
    Accession Version XM_020711740.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1491408000000
    Organism Oryzias latipes(Japanese medaka)
    Product collagen alpha-6(IV) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGTGACGTG GGTTTGCCCG GTGAGCCCGG CCAGCCTCTC
    ATCAATGGGA TTGTGGAGTT TGTGGGTTAT CCCAAAGGAG ACAAAGGAGA GCAGGGGGAG
    CCAGGTCCAA AAGGATCAAT TGGATTTCAA GGATTACCTG GACTGCCTGG AGCCTTTGGT
    TATCCAGGTG AAATTGGAGA GAAAGGCATC CCTGGATATC CTGGAGGAAG AGGTTCTCCA
    GGTTTTAATG GACCTCAGGG TGCTCCAGGA TTACCGGGAC AGAGAGGAGA CACAGGTTAC
    TCCGGCAGGC CAGGTTACGA CGGGCCAAAG GGCGACCCAG GAGACCGAGG TCCTCGAGGT
    CCTCCTATTT ATGTCAGTAA TATTGGCAGA ACTGCTCAAG GTCCTTCAGG AGATCCTGGT
    TACAGCGGTG CTCCTGGCGC TCCCGGAGAC CCAGGACCCA CAGGTTTCCC AGGACTTCCA
    GGTGAACCTG GACGTCTTCT GCCTGGTTCA GAAACATCAG GACTTCCAGG TCTACCCGGA
    CTACCTGGCC CGGAAGGAGA GAAGGGAAAC CCAGGTCCAC TGGTTAATGG TCCTGAAGGA
    TTCCCAGGTC TGCCTGGACC CCCTGGAGGT CCTGGCCCCC CGGGTCCTCC TGGCTCTCCT
    GAGGATGTGA CCTCCGGTCT ACCCGGAGTA CCCGGGCAGC CTGGCCCCAG AGGACCTCCT
    GGAGACCGAG GTTTCAAAGG CTTCGGAGGT GACCCTGGTG ACTGTGACTG TGAGGGAGGG
    GGCTCCTCAG GGGCAACTGG GTTACCTGGA CCTCCTGGAC TGAATGGCAG CCCTGGTGCC
    ACTGGAAGGA AGGGTGAACA GGGTGACACT GGATCATCGG GGTTCAGCGG GCCTTCTGGT
    TCTGATGGGC GGCCTGGTAG CAGGGGCTTC TTCGGTTTGA AAGGAGAAAA GGGGGAACCA
    TATTTATCAC GGGGCTCTTC GAGCAAGGGG GATCGTGGAG CACCCGGTCC CAGAGGACCC
    ATAGGTGAAA CTGGAAGACC TGGCAGAGAT GGGCTGCCTG GATTTGCTGG ACTTCCTGGA
    AGTCCTGGCG ACCCTGGCTT TGGTACAGTT GGAGAAAAGG GATTCTTAGG GTTCCCGGGA
    ATCAAAGGTC GTCCTGGAGC CCCTGGAGAG TCCGGCATTG GCTACATTGG ACTGCCTGGC
    TTGCGAGGAG AGCCTGGAGA CCCAGGTTTT TCTGGGTTGC CTGGGCCACC AGGTTTACCT
    GGACCAAGAG GTGAAAGCGC CTGTGGACGG GTTAATGGCG CCAAAACGGG AACGGGTGAT
    TTGTTGCCCT GCAAAATACC TGGACAAGTC GGAGACCCTG GGATTCCTGG CTTACCTGGA
    CGCCCTGGCC TTAAGGGTCA GCCGGGTCGG CCGGGAGAAC CTGGACGTCC AGGACTTGAT
    GGTTCCAAAG GGGAGAGGGG AGATCCTGGC TTTGGGAACC AGCCCGGTTC TCCAGGTTTT
    CCAGGACCTA GAGGGGACCC AGGAATCCCA GGTGTTCCTG GGCTGCGCAT TGATGGGACC
    AGGGGAAGAA ATGGTGTTCC AGGGCTTCCT GGAGCCAAAG GACAGCCTGG AGAGATTCTT
    GGAGCCATAC CTGGACTTCC AGGACGAGAT GGTGAACCTG GAATACGTGG GGACAAGGGC
    CAGCCCGGCT CTCCAGGAGT TCCTGGAGGT CCTGGTCTGG ATGGAAAGAA CGGTATTCCT
    GGACTGAGAG GAGAACGTGG AATTGATGGT CTGCCGGGTG TTCCTGGTTT TCCTGGACCT
    CCGGGTGAGG TTATCGGCCC TACTCTTGGA CTGCCTGGAC CTCCTGGCAG CAAAGGACAG
    ATTGGATCAC CGGGCTGCCC AGGTCTTCCT GGGGAGAAAG GAGACACAGG GGATCCAGGT
    TTCCCAGGAC CTGCAGGAAT GAAAGGAACA CCTGGGTTTC CTGGTACTCC TGGAACACCA
    GGTCCACGGG GACCGCCTGG ACCTCCAGGA CCTGGAACTG TTGACGGATT ACCTGGAATT
    CCTGGAGTAA AAGGGGAAAG AGGTTTTGTT GGACTCACTG GTTTTCCTGG ACAGTCTGGA
    CGGCCCGGAA CACCAGGATT TCCAGGAGGG AAAGGTCTGC CGGGGGAGCC TGGAAGAAAT
    GGATCACCTG GCAGACTTGG ACCTCAAGGC AACAGAGGTG ACAGAGGGCT TCCAGGTCTT
    CCAGGCCCCC CCAGTATTTA CACACCAGTT GTGGCGAAGG GAGAACTGGG AGATCCTGGA
    GCCAGAGGGT TTGATGGTTT TCCTGGACCC AGAGGTGACA AGGGTCTGCC TGGCCTGCCC
    GGTCGACAAG GTCGCCCTGG TCTTCCTGGT CTACCTGAGC AAAGTAAAGG GCAACCCGGG
    CAGCCGGGCC TACCTGGACT GCCGGGATCT CCAGGCTTAC CGGGACCAAA GGGAGAAGTT
    GGAATCAATG GGTTCCCTGG CATGCCAGGA CCAAGAGGAG ATGATGGAAT ACCTGGTGCA
    CCCGGAGGTC CTGGAGAACC AGGTCGTCCA GGTGGAAAAG GTCAACCTGG GGAAACCTTT
    GGTTTTCCTG GCTCACCTGG ACAGAAAGGT CAACCGGGAG ATCCAGGCAG CCCAGGACGT
    CAAGGCTTTG ATGGAGCCCC CGGTGATAAT GGCTTGTCTG GAAACCCTGG TTTCCCTGGA
    ATAAAAGGTG GTCCTGGTGA ACCAGGACGG CCTGGATTAC CTGGATCTGT TGGTTTTCCA
    GGCCCTAAAG GGCTGTCAAC ATTCCCTGGA AGAAAAGGAG CAGATGGGCC CCCAGGACAG
    CCTGGATTTC CAGGAGGTCC AGGAGCCAGG GGTGTGCCTG GACCTCCTGG CCTGGATGGA
    CTCAATGGCC TCGCTGGATC CAAAGGCCTT CCTGGAACCC CAGGTATTAC TTTAGGAGGT
    CGCCCTGGTG CTCCTGGTAT CCCAGGATTG AAGGGAGAGA GGGGAGATTC TTATCCTGGA
    CAACGAGGCT TTGATGGAGT AAAAGGACAA AAAGGAGAGC CTGGTCTTCC TGGAACTTCA
    GGAATTCCTG GTCAACCTGG ACTTCCTGGA GAGTCAGCGT TAGCAGGCAT TGCTGGACCT
    GTGGGGGACC CAGGACTTCC TGGATTGGAT GGAAACTATG GTTTTCAAGG TCCACCTGGT
    CCTCCTGGTC CCCCGGGTTC AGGAACAATA CAAGGAGAAA GAGGTGAACC AGGGCTGCCA
    GGCTTCCCGG GATCTCCTGG AAGAAAGGGA GAACCCGGTT TCAATGGAGG CCCTGGATAC
    CCAGGTAGCG CCGGTGTCAA AGGATCTCCT GGTGAAAGTG GTTTTAATGG ACGTCCAGGT
    CTTAAAGGTG TGCCTGGAGA TCCTGGCTTC TATGGAATCA AGGGATCTAA AGGAACACCA
    GGCCAAAAAG GACGGAAGGG TCCTCCTGGA GTTGTGCTGC CTTCCCCTAT TGTGGAATCA
    CAGCCTTCTG GCGACATGGG TCTTCCTGGT GAAAGGGGAC CCTTGGGTTA CCGTGGAGAC
    TCTGGTTGGA GTGGCATTCC AGGGCGTCCT GGTCCAAAAG GACGACCCGG AGCCCCAGGT
    AACCTTGGAA GGACTGGACC GTCTGGTCTG CCTGGTCCGT TAGGTGATCC CGGCTCCCCT
    GGTTTACCTG GAACAAGTGG AAGACAAGGC CCCCCTGGAA ACATTGGCCT CCCTGGCATT
    CCTGGCAGCT CCAGCCGCAG TATCAGCATT GGCTACACTC TGGTAAAGCA CAGCCAGAGC
    GAGCAGGTCC CCATGTGTCC TCAGGGCATG CCTTCATTGT GGAATGGGTA CAGCTTGCTG
    TATGTGGAGG GACAGGAAAA AGCACACAAC CAAGACCTGG GTCAGCCTGG CTCGTGCCTC
    CCCAGGTTCA GCACCATGCC CTTCCTGTAC TGCTCCCCCA ATGAGGTTTG CTTCTTTGCT
    AGTCGCAATG ACAAATCCTA CTGGCTCTCC ACATCTGCAC CCATTCCCAT GATGCCCGTG
    TCTGCGGGGC AGATCCAGCC TTACATCAGC AGATGTTCAG TATGTGAGGC ACCATCTCAG
    GCTGTGGCGG TCCACAGTCA GGACATGACC ATCCCCATGT GCCCCCCTGG GTGGAGAAGT
    CTTTGGATTG GATATTCCTT CTTGATGCAC ACGGCGGCTG GTGCAGAAGG CGGCGGTCAG
    TCCCTGATGT CTCCCGGCTC CTGCTTGGAG GATTTCCGCG CCACTCCATT CATTGAGTGC
    AATGGAGCCA AAGGCACATG CCACTACTTT GCCAACAAAT ACAGCTTCTG GCTGACCACC
    GTGGATCCCA AGCGGGAGTT TGTGTCTTCA CCAAACCAGG AGACCCTGAA GGCGGGCCAG
    GAGCGCTCCA GAGTCAGTCG CTGTCAGGTC TGCAGCAAAC TGCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020567399.1
    CDS489..5576
    Translation

    Target ORF information:

    RefSeq Version XM_020711740.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes collagen alpha-6(IV) chain (LOC101168576), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_020711740.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGTCTTC TGCCGTGCGT GACGGTGATC ACCGCCCTGC TGCTGCAGCT TGGACCCGCG 
    CGCGGAGGGG ACAAGTTTGA TGAGCCATGC AACGGCCTGG ACTGCAGCAG GGGGTGCCAG
    TGTCTGCCTC AGAAAGGAGC ACGGGGTCAA CCAGGGCTTC TTGGTGAATC TGGTCCCAGT
    GGTCCAGAGG GACCACAAGG GGGACTGGGC CCTCGAGGGC CAAAGGGAGA GATGGGTCAT
    GTTGGACGTA AAGGACCATC TGGGCCTAAA GGAGATAAAG GACCAATGGG AGTACCAGGA
    TTTCGAGGGA GTAAAGGCAG TGACGGTCAT CCCGGTCAGC CAGGTGAGAG GGGGTTACCT
    GGGTTCGATG GATGTAACGG GACGAGAGGA GATCCCGGTC TGCCTGGTTA TGGAACAGGA
    GCTCCTGGAT TCCCAGGACG TCCTGGTCCA GCTGGGCCGA AGGGTCAGAA AGGAGAGCCC
    ACTTACTACA CTGTGTCCAG GAGTTTGCCG GGGCCTCCTG GACTGAGAGG TCCAACTGGC
    TTCCGTGGTC CCGATGGTCC TCAAGGTTTT CCTGGTCCAA GTGGACCTGA AGGATTCCCG
    GGCCTCCCCG GTCCTCCTGG CCCCCCAGGC CCAATAGAAA CCGGCTTTGA CGGACAGGGA
    GCAGGAGAAA AGGGAGATAA GGGTGACGTG GGTTTGCCCG GTGAGCCCGG CCAGCCTCTC
    ATCAATGGGA TTGTGGAGTT TGTGGGTTAT CCCAAAGGAG ACAAAGGAGA GCAGGGGGAG
    CCAGGTCCAA AAGGATCAAT TGGATTTCAA GGATTACCTG GACTGCCTGG AGCCTTTGGT
    TATCCAGGTG AAATTGGAGA GAAAGGCATC CCTGGATATC CTGGAGGAAG AGGTTCTCCA
    GGTTTTAATG GACCTCAGGG TGCTCCAGGA TTACCGGGAC AGAGAGGAGA CACAGGTTAC
    TCCGGCAGGC CAGGTTACGA CGGGCCAAAG GGCGACCCAG GAGACCGAGG TCCTCGAGGT
    CCTCCTATTT ATGTCAGTAA TATTGGCAGA ACTGCTCAAG GTCCTTCAGG AGATCCTGGT
    TACAGCGGTG CTCCTGGCGC TCCCGGAGAC CCAGGACCCA CAGGTTTCCC AGGACTTCCA
    GGTGAACCTG GACGTCTTCT GCCTGGTTCA GAAACATCAG GACTTCCAGG TCTACCCGGA
    CTACCTGGCC CGGAAGGAGA GAAGGGAAAC CCAGGTCCAC TGGTTAATGG TCCTGAAGGA
    TTCCCAGGTC TGCCTGGACC CCCTGGAGGT CCTGGCCCCC CGGGTCCTCC TGGCTCTCCT
    GAGGATGTGA CCTCCGGTCT ACCCGGAGTA CCCGGGCAGC CTGGCCCCAG AGGACCTCCT
    GGAGACCGAG GTTTCAAAGG CTTCGGAGGT GACCCTGGTG ACTGTGACTG TGAGGGAGGG
    GGCTCCTCAG GGGCAACTGG GTTACCTGGA CCTCCTGGAC TGAATGGCAG CCCTGGTGCC
    ACTGGAAGGA AGGGTGAACA GGGTGACACT GGATCATCGG GGTTCAGCGG GCCTTCTGGT
    TCTGATGGGC GGCCTGGTAG CAGGGGCTTC TTCGGTTTGA AAGGAGAAAA GGGGGAACCA
    TATTTATCAC GGGGCTCTTC GAGCAAGGGG GATCGTGGAG CACCCGGTCC CAGAGGACCC
    ATAGGTGAAA CTGGAAGACC TGGCAGAGAT GGGCTGCCTG GATTTGCTGG ACTTCCTGGA
    AGTCCTGGCG ACCCTGGCTT TGGTACAGTT GGAGAAAAGG GATTCTTAGG GTTCCCGGGA
    ATCAAAGGTC GTCCTGGAGC CCCTGGAGAG TCCGGCATTG GCTACATTGG ACTGCCTGGC
    TTGCGAGGAG AGCCTGGAGA CCCAGGTTTT TCTGGGTTGC CTGGGCCACC AGGTTTACCT
    GGACCAAGAG GTGAAAGCGC CTGTGGACGG GTTAATGGCG CCAAAACGGG AACGGGTGAT
    TTGTTGCCCT GCAAAATACC TGGACAAGTC GGAGACCCTG GGATTCCTGG CTTACCTGGA
    CGCCCTGGCC TTAAGGGTCA GCCGGGTCGG CCGGGAGAAC CTGGACGTCC AGGACTTGAT
    GGTTCCAAAG GGGAGAGGGG AGATCCTGGC TTTGGGAACC AGCCCGGTTC TCCAGGTTTT
    CCAGGACCTA GAGGGGACCC AGGAATCCCA GGTGTTCCTG GGCTGCGCAT TGATGGGACC
    AGGGGAAGAA ATGGTGTTCC AGGGCTTCCT GGAGCCAAAG GACAGCCTGG AGAGATTCTT
    GGAGCCATAC CTGGACTTCC AGGACGAGAT GGTGAACCTG GAATACGTGG GGACAAGGGC
    CAGCCCGGCT CTCCAGGAGT TCCTGGAGGT CCTGGTCTGG ATGGAAAGAA CGGTATTCCT
    GGACTGAGAG GAGAACGTGG AATTGATGGT CTGCCGGGTG TTCCTGGTTT TCCTGGACCT
    CCGGGTGAGG TTATCGGCCC TACTCTTGGA CTGCCTGGAC CTCCTGGCAG CAAAGGACAG
    ATTGGATCAC CGGGCTGCCC AGGTCTTCCT GGGGAGAAAG GAGACACAGG GGATCCAGGT
    TTCCCAGGAC CTGCAGGAAT GAAAGGAACA CCTGGGTTTC CTGGTACTCC TGGAACACCA
    GGTCCACGGG GACCGCCTGG ACCTCCAGGA CCTGGAACTG TTGACGGATT ACCTGGAATT
    CCTGGAGTAA AAGGGGAAAG AGGTTTTGTT GGACTCACTG GTTTTCCTGG ACAGTCTGGA
    CGGCCCGGAA CACCAGGATT TCCAGGAGGG AAAGGTCTGC CGGGGGAGCC TGGAAGAAAT
    GGATCACCTG GCAGACTTGG ACCTCAAGGC AACAGAGGTG ACAGAGGGCT TCCAGGTCTT
    CCAGGCCCCC CCAGTATTTA CACACCAGTT GTGGCGAAGG GAGAACTGGG AGATCCTGGA
    GCCAGAGGGT TTGATGGTTT TCCTGGACCC AGAGGTGACA AGGGTCTGCC TGGCCTGCCC
    GGTCGACAAG GTCGCCCTGG TCTTCCTGGT CTACCTGAGC AAAGTAAAGG GCAACCCGGG
    CAGCCGGGCC TACCTGGACT GCCGGGATCT CCAGGCTTAC CGGGACCAAA GGGAGAAGTT
    GGAATCAATG GGTTCCCTGG CATGCCAGGA CCAAGAGGAG ATGATGGAAT ACCTGGTGCA
    CCCGGAGGTC CTGGAGAACC AGGTCGTCCA GGTGGAAAAG GTCAACCTGG GGAAACCTTT
    GGTTTTCCTG GCTCACCTGG ACAGAAAGGT CAACCGGGAG ATCCAGGCAG CCCAGGACGT
    CAAGGCTTTG ATGGAGCCCC CGGTGATAAT GGCTTGTCTG GAAACCCTGG TTTCCCTGGA
    ATAAAAGGTG GTCCTGGTGA ACCAGGACGG CCTGGATTAC CTGGATCTGT TGGTTTTCCA
    GGCCCTAAAG GGCTGTCAAC ATTCCCTGGA AGAAAAGGAG CAGATGGGCC CCCAGGACAG
    CCTGGATTTC CAGGAGGTCC AGGAGCCAGG GGTGTGCCTG GACCTCCTGG CCTGGATGGA
    CTCAATGGCC TCGCTGGATC CAAAGGCCTT CCTGGAACCC CAGGTATTAC TTTAGGAGGT
    CGCCCTGGTG CTCCTGGTAT CCCAGGATTG AAGGGAGAGA GGGGAGATTC TTATCCTGGA
    CAACGAGGCT TTGATGGAGT AAAAGGACAA AAAGGAGAGC CTGGTCTTCC TGGAACTTCA
    GGAATTCCTG GTCAACCTGG ACTTCCTGGA GAGTCAGCGT TAGCAGGCAT TGCTGGACCT
    GTGGGGGACC CAGGACTTCC TGGATTGGAT GGAAACTATG GTTTTCAAGG TCCACCTGGT
    CCTCCTGGTC CCCCGGGTTC AGGAACAATA CAAGGAGAAA GAGGTGAACC AGGGCTGCCA
    GGCTTCCCGG GATCTCCTGG AAGAAAGGGA GAACCCGGTT TCAATGGAGG CCCTGGATAC
    CCAGGTAGCG CCGGTGTCAA AGGATCTCCT GGTGAAAGTG GTTTTAATGG ACGTCCAGGT
    CTTAAAGGTG TGCCTGGAGA TCCTGGCTTC TATGGAATCA AGGGATCTAA AGGAACACCA
    GGCCAAAAAG GACGGAAGGG TCCTCCTGGA GTTGTGCTGC CTTCCCCTAT TGTGGAATCA
    CAGCCTTCTG GCGACATGGG TCTTCCTGGT GAAAGGGGAC CCTTGGGTTA CCGTGGAGAC
    TCTGGTTGGA GTGGCATTCC AGGGCGTCCT GGTCCAAAAG GACGACCCGG AGCCCCAGGT
    AACCTTGGAA GGACTGGACC GTCTGGTCTG CCTGGTCCGT TAGGTGATCC CGGCTCCCCT
    GGTTTACCTG GAACAAGTGG AAGACAAGGC CCCCCTGGAA ACATTGGCCT CCCTGGCATT
    CCTGGCAGCT CCAGCCGCAG TATCAGCATT GGCTACACTC TGGTAAAGCA CAGCCAGAGC
    GAGCAGGTCC CCATGTGTCC TCAGGGCATG CCTTCATTGT GGAATGGGTA CAGCTTGCTG
    TATGTGGAGG GACAGGAAAA AGCACACAAC CAAGACCTGG GTCAGCCTGG CTCGTGCCTC
    CCCAGGTTCA GCACCATGCC CTTCCTGTAC TGCTCCCCCA ATGAGGTTTG CTTCTTTGCT
    AGTCGCAATG ACAAATCCTA CTGGCTCTCC ACATCTGCAC CCATTCCCAT GATGCCCGTG
    TCTGCGGGGC AGATCCAGCC TTACATCAGC AGATGTTCAG TATGTGAGGC ACCATCTCAG
    GCTGTGGCGG TCCACAGTCA GGACATGACC ATCCCCATGT GCCCCCCTGG GTGGAGAAGT
    CTTTGGATTG GATATTCCTT CTTGATGCAC ACGGCGGCTG GTGCAGAAGG CGGCGGTCAG
    TCCCTGATGT CTCCCGGCTC CTGCTTGGAG GATTTCCGCG CCACTCCATT CATTGAGTGC
    AATGGAGCCA AAGGCACATG CCACTACTTT GCCAACAAAT ACAGCTTCTG GCTGACCACC
    GTGGATCCCA AGCGGGAGTT TGTGTCTTCA CCAAACCAGG AGACCCTGAA GGCGGGCCAG
    GAGCGCTCCA GAGTCAGTCG CTGTCAGGTC TGCAGCAAAC TGCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.