LOC101162572 cDNA ORF clone, Oryzias latipes(Japanese medaka)

The following LOC101162572 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101162572 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OJa68005 XM_020700652.1
Latest version!
Oryzias latipes serine/threonine-protein kinase WNK4-like (LOC101162572), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OJa117518 XM_023957165.1
Latest version!
Oryzias latipes serine/threonine-protein kinase WNK1 (LOC101162572), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OJa117519 XM_023957166.1
Latest version!
Oryzias latipes serine/threonine-protein kinase WNK1 (LOC101162572), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OJa117520 XM_023957167.1
Latest version!
Oryzias latipes serine/threonine-protein kinase WNK1 (LOC101162572), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OJa67999 XM_020700646.1
Latest version!
Oryzias latipes serine/threonine-protein kinase WNK4-like (LOC101162572), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OJa68000 XM_020700647.1
Latest version!
Oryzias latipes serine/threonine-protein kinase WNK4-like (LOC101162572), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OJa68001 XM_020700648.1
Latest version!
Oryzias latipes serine/threonine-protein kinase WNK4-like (LOC101162572), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OJa68002 XM_020700649.1
Latest version!
Oryzias latipes serine/threonine-protein kinase WNK4-like (LOC101162572), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OJa68003 XM_020700650.1
Latest version!
Oryzias latipes serine/threonine-protein kinase WNK4-like (LOC101162572), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OJa68004 XM_020700651.1
Latest version!
Oryzias latipes serine/threonine-protein kinase WNK4-like (LOC101162572), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OJa117513 XM_023957160.1
Latest version!
Oryzias latipes serine/threonine-protein kinase WNK1 (LOC101162572), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OJa117514 XM_023957161.1
Latest version!
Oryzias latipes serine/threonine-protein kinase WNK1 (LOC101162572), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OJa117515 XM_023957162.1
Latest version!
Oryzias latipes serine/threonine-protein kinase WNK1 (LOC101162572), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OJa117516 XM_023957163.1
Latest version!
Oryzias latipes serine/threonine-protein kinase WNK1 (LOC101162572), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OJa117517 XM_023957164.1
Latest version!
Oryzias latipes serine/threonine-protein kinase WNK1 (LOC101162572), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OJa68005
    Clone ID Related Accession (Same CDS sequence) XM_020700652.1
    Accession Version XM_020700652.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2694bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1491408000000
    Organism Oryzias latipes(Japanese medaka)
    Product serine/threonine-protein kinase WNK4-like isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004090421.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACGACA AGGAGAAGCT CCGGGGGAAG TATAAAGACA ACGACGCCAT CGAGTTCCTG 
    TACGAACTCT ACAAGGATGT GCCAGAGGAG GTCGCCCAGG AGATGGTCAT TTTGGGATTT
    GTGTTCAAAG CAGACTACAA GGTGATTGCC AAAGCCATTC GGCATCGAGT GACAGCCATA
    AAGTATCAGC GGGAAAAACG GCGCCTCCTG CTGGAGGAAG GAGTGAACAG TCAGAAGCAA
    ACTGACATCG AGGAAGAGCT TCATCTTCAT CAACGAGCTG AGCTGTCCAA TCAGACGCTC
    CCAGTTTCAA CAGGAGCCAG AGGAGCGGCC AGTCAGGGGG ACTCTGCTGC ATCCACACAA
    GCTCCGCCCC TGACTGCAAC AGCACAAGCT CCGCCCCTGA CTGCATCAGC ACAAGCTCCG
    CCTCTGACTG CAACAGCACA AGCTCCGCCC CTGACTGCAT CAGCACAAGC TCCGCCCCTG
    ACTGCAACAG CACAAGCTCC GCCCCTGACT GCATCAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CACGCTCCTT CTCTAACTGC ATCGGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCCAC ACACGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGATTGCAT CCACACACTC TCCTTCCCTA ACTGCATCGG CACAAGCTCC ACCCCTGACT
    GCATCCACAC ACGCTCCTTC CCTAACTGCA TCGGCACAAG CTCCGCCCCT GACTGCATCG
    GCACAAACTC CGCCCCTGAC TGCATCGGCA CAAGCTCCGC CCCTGACTGC ATCAGCACAA
    GCTCCGCCCC TGACTGCATC CACACACGCT CCGCCCCTGA CTTTATCAGC ACAAGCTCCG
    CCCCTGACTG CATCAGCACA AGCTCCGCCC CTGACTGCAT CCACACAAGC TCCGCCCAGC
    AGCCGGGGGG ATTCCGGGAT CAACACTGTC TCCAGCAGAG CTGATGGCGA CACCGAGGAG
    GACGGTGCGA TCCGACTAGT CTCCCACCCC CCCACTGCCT CAGACCATGT GATTGAAACC
    ACGCCTCTTC TTGTTCCTAA CCCCACTCCT CCATCAGTAC CTCCACAGAC GGAGGTTCCT
    TCTGTTTTGA CCCGCACAGC TTCCAAAGCT CCGCCCTTTC CCATACTACG CTTTCCTAAA
    AGCATTGCTG TGTCCCAGGC TGCAGTAAAG CCCCCCCCTG AGCCCTCCTA TGATTTCTCT
    TCACCTGTCG ACAGCTGCTC TTCTGACTTC ACCTCGGGCT TGAGTGATGG CAATGACGGC
    CAATCAGAGA GGAGCAGGAT GGAGGCAACG ATGAGGGAAG CAACCAAACA GTTTAGAAGG
    AGAGCCAGAG CTCGTCTCCG AATCATAGGG GTGTCTGACA AGGTGGACCG AGTGGTGGAG
    TGTCAGCTGC AGACTCACAA CAACAAGATG GTCACGTTTA AGTTTGACCT GGATGGAGAC
    AATCCCGAGG ACATCGCATC TGTGATGATT CACAGAGATT TTATCTTACC GTCTGAGCGA
    GAAGGATTTA TCCTCCGCAT GTATGACATC ATCCAGAGGG CGGAGTCTAT GATACACCAG
    CATCATCCTG CAGTCTTGTA CCAGCCTTCT CAGCTGATGA CCTCTGCTCC CCAGTCAAAC
    TGCCTCTGCT TTCAACCTTT TCAGCCAAAG TCAGCAGTGA CTAACCTGTC CAGGACTTTG
    TCTTCCTCCT CCTTACCTGA CATAGATGAA AGTGACACCA CCCCCTCAAA GGCAATTGAC
    TTCAGCATTG ACCCTGATGC TGTTCCCCCC GTGAGGCCGT TGAGGTCACA GTCCTTCCAG
    ACGTCATCAG CCTCTTCCTA TGAGCCCCCC CTTTACCCTT ACCATTACCC CCATCCTGAA
    TCTACCACTC CCCCCCTCTC TCAGAATCTG CTTCCTTCTC ATCTTTACTC CACCTATACA
    GCCTCTTTCT CCACGCCCAG CTCCGCCCCC CAGATTCCAC GAGTCCACAG CAACCCCTCC
    CTCTTTACCT CCACACCATC ATCTTTAGCC CCTCCCTCTC CTAGGTGGCC ACCCCCTGAC
    CAGCCTCTCT TCTCTTTGGC CAATGTACTG TCTGTGGCCA TGAGTGTGGC CCACAGCCTC
    ATGCCCCCAC CCGGACATCC GAGTCAAGGC TTTCAACACC AGGCTCCTCC CCCGCCTTTC
    CCCCAGTCCA CGTTCTCACC CCCCATGTCT CCATCTCTAA AACGGAACCT TCAGACTTTG
    ACAAACACCT CCACCGCTGA ACCCCACCAA CCCCCCCAGG CCTATCACAC AGGCTTGGAG
    CCAGAAGTCC AAAAGACAGT GATGGACGGC GGAGCTGCAG ATCAGTGTCC CGGTCCTGCC
    CGACCACGGC TCGGCTCGGC TCCCTCCTCC CAGGGTTCAG AGACCTCCGT TCTTCCCCGT
    CAGGCCAGCC TCTCAAATCC CAGAGGAGAG TTTTCAGATT CCTCCACCTC GTCCTCAGCC
    TCCCCTCCTC CCCCATCGGG TTTCTACCCC AAAGTGTCCC CCCCAGTGTC CCCATCACGC
    TCCATCTCTC CTACGTTGAA AGACACTTGG TGGAGGTCCA GAATCTACAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020556311.1
    CDS228..2921
    Translation

    Target ORF information:

    RefSeq Version XM_020700652.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes serine/threonine-protein kinase WNK4-like (LOC101162572), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_020700652.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACGACA AGGAGAAGCT CCGGGGGAAG TATAAAGACA ACGACGCCAT CGAGTTCCTG 
    TACGAACTCT ACAAGGATGT GCCAGAGGAG GTCGCCCAGG AGATGGTCAT TTTGGGATTT
    GTGTTCAAAG CAGACTACAA GGTGATTGCC AAAGCCATTC GGCATCGAGT GACAGCCATA
    AAGTATCAGC GGGAAAAACG GCGCCTCCTG CTGGAGGAAG GAGTGAACAG TCAGAAGCAA
    ACTGACATCG AGGAAGAGCT TCATCTTCAT CAACGAGCTG AGCTGTCCAA TCAGACGCTC
    CCAGTTTCAA CAGGAGCCAG AGGAGCGGCC AGTCAGGGGG ACTCTGCTGC ATCCACACAA
    GCTCCGCCCC TGACTGCAAC AGCACAAGCT CCGCCCCTGA CTGCATCAGC ACAAGCTCCG
    CCTCTGACTG CAACAGCACA AGCTCCGCCC CTGACTGCAT CAGCACAAGC TCCGCCCCTG
    ACTGCAACAG CACAAGCTCC GCCCCTGACT GCATCAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CACGCTCCTT CTCTAACTGC ATCGGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCCAC ACACGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGATTGCAT CCACACACTC TCCTTCCCTA ACTGCATCGG CACAAGCTCC ACCCCTGACT
    GCATCCACAC ACGCTCCTTC CCTAACTGCA TCGGCACAAG CTCCGCCCCT GACTGCATCG
    GCACAAACTC CGCCCCTGAC TGCATCGGCA CAAGCTCCGC CCCTGACTGC ATCAGCACAA
    GCTCCGCCCC TGACTGCATC CACACACGCT CCGCCCCTGA CTTTATCAGC ACAAGCTCCG
    CCCCTGACTG CATCAGCACA AGCTCCGCCC CTGACTGCAT CCACACAAGC TCCGCCCAGC
    AGCCGGGGGG ATTCCGGGAT CAACACTGTC TCCAGCAGAG CTGATGGCGA CACCGAGGAG
    GACGGTGCGA TCCGACTAGT CTCCCACCCC CCCACTGCCT CAGACCATGT GATTGAAACC
    ACGCCTCTTC TTGTTCCTAA CCCCACTCCT CCATCAGTAC CTCCACAGAC GGAGGTTCCT
    TCTGTTTTGA CCCGCACAGC TTCCAAAGCT CCGCCCTTTC CCATACTACG CTTTCCTAAA
    AGCATTGCTG TGTCCCAGGC TGCAGTAAAG CCCCCCCCTG AGCCCTCCTA TGATTTCTCT
    TCACCTGTCG ACAGCTGCTC TTCTGACTTC ACCTCGGGCT TGAGTGATGG CAATGACGGC
    CAATCAGAGA GGAGCAGGAT GGAGGCAACG ATGAGGGAAG CAACCAAACA GTTTAGAAGG
    AGAGCCAGAG CTCGTCTCCG AATCATAGGG GTGTCTGACA AGGTGGACCG AGTGGTGGAG
    TGTCAGCTGC AGACTCACAA CAACAAGATG GTCACGTTTA AGTTTGACCT GGATGGAGAC
    AATCCCGAGG ACATCGCATC TGTGATGATT CACAGAGATT TTATCTTACC GTCTGAGCGA
    GAAGGATTTA TCCTCCGCAT GTATGACATC ATCCAGAGGG CGGAGTCTAT GATACACCAG
    CATCATCCTG CAGTCTTGTA CCAGCCTTCT CAGCTGATGA CCTCTGCTCC CCAGTCAAAC
    TGCCTCTGCT TTCAACCTTT TCAGCCAAAG TCAGCAGTGA CTAACCTGTC CAGGACTTTG
    TCTTCCTCCT CCTTACCTGA CATAGATGAA AGTGACACCA CCCCCTCAAA GGCAATTGAC
    TTCAGCATTG ACCCTGATGC TGTTCCCCCC GTGAGGCCGT TGAGGTCACA GTCCTTCCAG
    ACGTCATCAG CCTCTTCCTA TGAGCCCCCC CTTTACCCTT ACCATTACCC CCATCCTGAA
    TCTACCACTC CCCCCCTCTC TCAGAATCTG CTTCCTTCTC ATCTTTACTC CACCTATACA
    GCCTCTTTCT CCACGCCCAG CTCCGCCCCC CAGATTCCAC GAGTCCACAG CAACCCCTCC
    CTCTTTACCT CCACACCATC ATCTTTAGCC CCTCCCTCTC CTAGGTGGCC ACCCCCTGAC
    CAGCCTCTCT TCTCTTTGGC CAATGTACTG TCTGTGGCCA TGAGTGTGGC CCACAGCCTC
    ATGCCCCCAC CCGGACATCC GAGTCAAGGC TTTCAACACC AGGCTCCTCC CCCGCCTTTC
    CCCCAGTCCA CGTTCTCACC CCCCATGTCT CCATCTCTAA AACGGAACCT TCAGACTTTG
    ACAAACACCT CCACCGCTGA ACCCCACCAA CCCCCCCAGG CCTATCACAC AGGCTTGGAG
    CCAGAAGTCC AAAAGACAGT GATGGACGGC GGAGCTGCAG ATCAGTGTCC CGGTCCTGCC
    CGACCACGGC TCGGCTCGGC TCCCTCCTCC CAGGGTTCAG AGACCTCCGT TCTTCCCCGT
    CAGGCCAGCC TCTCAAATCC CAGAGGAGAG TTTTCAGATT CCTCCACCTC GTCCTCAGCC
    TCCCCTCCTC CCCCATCGGG TTTCTACCCC AAAGTGTCCC CCCCAGTGTC CCCATCACGC
    TCCATCTCTC CTACGTTGAA AGACACTTGG TGGAGGTCCA GAATCTACAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa117518
    Clone ID Related Accession (Same CDS sequence) XM_023957165.1
    Accession Version XM_023957165.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1518537600000
    Organism Oryzias latipes(Japanese medaka)
    Product serine/threonine-protein kinase WNK1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019866.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGCACT CCGCTGAAGA TGAAGAAGAG GAGAACTCTG CTCTTCATCT CAAGGACTCC 
    GACCAATCAG AGGCTTCCGA TCCACACACA GGTCATGATG TCAGCTCAGA CTCTGCCGAT
    GAAATGGGCA TCAATCTCTC AGAATTAAAC AGTGAGATAT TACCATGGCA ACGCACCCAG
    GAGGAGGAGC ACGACGAAGA GGAAATGAAC GCTGTGGCGT CGTCGCCTGA TGGGCGGTTC
    CTGAAGTTCA ACATCGAGAT CGGGAGGGGC TCCTTCAAAA CCGTTTACAA AGGGCTGGAC
    ACGGAGACCA CAGTGGAGGT GGCCTGGTGC GAGCTCCAGA CCCACCGGCT GAGCAGGGCC
    GAGCGTCAGC GTTTCAGCGA GGAGGTGGAG ATGCTCAAAG CGCTTCAGCA CCCCAACATC
    GTCCGTTTCT TCGATTCCTG GAAGTCCGCG CTGAAGGGCC ACAAATGCAC CATCCTGGTG
    ACGGAGCTCA TGACGTCTGG AACGCTGAAG ATGTACCTGC GCAGGTTCCG TCAGATGAAG
    CTGAAGCTGC TGCAGCGCTG GAGCTTCCAG GTCCTGCGGG GGCTTCACTT CCTGCACTCA
    CGTTCCCCCC CCATCATACA CCGTGACCTC AAATGTGACA ACATCTTCAT CACGGGTCCC
    TCCGCCTCCG TCAAAATCGG AGACCTGGGC CTGGCAACGC TGAAGAAGGC CTCGTTTGCT
    AAGAGCGTGA TCGGAACGCC GGAGTTCATG GCTCCAGAGA TGTACGAGGA GAAGTACGAC
    GAGGCTGTGG ACGTGTACGC CTTCGGCATG TGCATGCTGG AGATGGCTAC GTCCGAGTAT
    CCGTACCACG AGTGTCACAG CGCTGCCCAG ATCTACCGCA AGGTCACCAA TGGAATCAAA
    CCGGACAGTT TCTTCAAAGT GGAAGTTCCA GAACTGAAAG AAATCATTGA AGGCTGCATT
    CGGACCAGGA GCAGTGAAAG GTTTACAGTC CAGGACCTCT TGGAGCACAG CTTCTTTCAG
    GAGAACACCG GCGTCCATGT GGAGCTGGCA GAGGAAGACG ACGGCTCCAA GGCGGCGCTG
    AAGCTGTGGC TCCGGATGGA CGACAAGGAG AAGCTCCGGG GGAAGTATAA AGACAACGAC
    GCCATCGAGT TCCTGTACGA ACTCTACAAG GATGTGCCAG AGGAGGTCGC CCAGGAGATG
    GTCATTTTGG GATTTGTGTT CAAAGCAGAC TACAAGGTGA TTGCCAAAGC CATTCGGCAT
    CGAGTGACAG CCATAAAGTA TCAGCGGGAA AAACGGCGCC TCCTGCTGGA GGAAGGAGTG
    AACAGTCAGA AGCAAACTGA CATCGAGGAA GAGCTTCATC TTCATCAACG AGCTGAGCTG
    TCCAATCAGA CGCTCCCAGT TTCAACAGGA GCCAGAGGAG CGGCCAGTCA GGGGGACTCT
    GCTGCATCCA CACAAGCTCC GCCCCTGACT GCAACAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCTCT GACTGCAACA GCACAAGCTC CGCCCCTGAC TGCATCAGCA
    CAAGCTCCGC CCCTGACTGC AACAGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCAGC ACAAGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGACTGCAT CCACACACGC TCCTTCTCTA ACTGCATCGG CACAAGCTCC GCCCCTGACT
    GCATCAGCAC AAGCTCCGCC CCTGACTGCA TCCACACACG CTCCGCCCCT GACTGCATCA
    GCACAAGCTC CGCCCCTGAT TGCATCCACA CACTCTCCTT CCCTAACTGC ATCGGCACAA
    GCTCCACCCC TGACTGCATC CACACACGCT CCTTCCCTAA CTGCATCGGC ACAAGCTCCG
    CCCCTGACTG CATCGGCACA AACTCCGCCC CTGACTGCAT CGGCACAAGC TCCGCCCCTG
    ACTGCATCAG CACAAGCTCC GCCCCTGACT GCATCCACAC ACGCTCCGCC CCTGACTTTA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CAAGCTCCGC CCAGCAGCCG GGGGGATTCC GGGATCAACA CTGTCTCCAG CAGAGCTGAT
    GGCGACACCG AGGAGGACGG TGCGATCCGA CTAGTCTCCC ACCCCCCCAC TGCCTCAGAC
    CATGTGATTG AAACCACGCC TCTTCTTGTT CCTAACCCCA CTCCTCCATC AGTACCTCCA
    CAGACGGAGG TTCCTTCTGT TTTGACCCGC ACAGCTTCCA AAGCTCCGCC CTTTCCCATA
    CTACGCTTTC CTAAAAGCAT TGCTGTGTCC CAGGCTGCAG TAAAGCCCCC CCCTGAGCCC
    TCCTATGATT TCTCTTCACC TGTCGACAGC TGCTCTTCTG ACTTCACCTC GGGCTTGAGT
    GATGGCAATG ACGGCCAATC AGAGAGGAGC AGGATGGAGG CAACGATGAG GGAAGCAACC
    AAACAGTTTA GAAGGAGAGC CAGAGCTCGT CTCCGAATCA TAGGGGTGTC TGACAAGGTG
    GACCGAGTGG TGGAGTGTCA GCTGCAGACT CACAACAACA AGATGGTCAC GTTTAAGTTT
    GACCTGGATG GAGACAATCC CGAGGACATC GCATCTGTGA TGATTCACAG AGATTTTATC
    TTACCGTCTG AGCGAGAAGG ATTTATCCTC CGCATGTATG ACATCATCCA GAGGGCGGAG
    TCTATGATAC ACCAGCATCA TCCTGCAGTC TTGTACCAGC CTTCTCAGCT GATGACCTCT
    GCTCCCCAGT CAAACTGCCT CTGCTTTCAA CCTTTTCAGC CAAAGTCAGC AGTGACTAAC
    CTGTCCAGGA CTTTGTCTTC CTCCTCCTTA CCTGACATAG ATGAAAGTGA CACCACCCCC
    TCAAAGGCAA TTGACTTCAG CATTGACCCT GATGCTGTTC CCCCCGTGAG GCCGTTGAGG
    TCACAGTCCT TCCAGACGTC ATCAGCCTCT TCCTATGAGC CCCCCCTTTA CCCTTACCAT
    TACCCCCATC CTGAATCTAC CACTCCCCCC CTCTCTCAGA ATCTGCTTCC TTCTCATCTT
    TACTCCACCT ATACAGCCTC TTTCTCCACG CCCAGCTCCG CCCCCCAGAT TCCACGAGTC
    CACAGCAACC CCTCCCTCTT TACCTCCACA CCATCATCTT TAGCCCCTCC CTCTCCTAGG
    TGGCCACCCC CTGACCAGCC TCTCTTCTCT TTGGCCAATG TACTGTCTGT GGCCATGAGT
    GTGGCCCACA GCCTCATGCC CCCACCCGGA CATCCGAGTC AAGGCTTTCA ACACCAGGCT
    CCTCCCCCGC CTTTCCCCCA GTCCACGTTC TCACCCCCCA TGTCTCCATC TCTAAAACGG
    AACCTTCAGA CTTTGACAAA CACCTCCACC GCTGAACCCC ACCAACCCCC CCAGGCCTAT
    CACACAGGCT TGGAGCCAGA AGTCCAAAAG ACAGTGATGG ACGGCGGAGC TGCAGATCAG
    TGTCCCGGTC CTGCCCGACC ACGGCTCGGC TCGGCTCCCT CCTCCCAGGG TTCAGAGACC
    TCCGTTCTTC CCCGTCAGGC CAGCCTCTCA AATCCCAGAG GAGAGTTTTC AGATTCCTCC
    ACCTCGTCCT CAGCCTCCCC TCCTCCCCCA TCGGGTTTCT ACCCCAAAGT GTCCCCCCCA
    GTGTCCCCAT CACGCTCCAT CTCTCCTACG TTGAAAGCGG GGAAAACAGC CTTCACTGTG
    GGCCGCTTCC AGGTGACCCC ATCAACACCC GTTTCTGCTG TCAGTCATCA TCAGCCACGC
    CCCTTTAGTG ATGCTTCACC CATCGCTCAC TCCCCCCCTC CTACTCAGAA GAACCAATCA
    GAGAGCAGCT CAAGAAGCAG TTCAGAAGAA GACAGCCAAT CAGAAATCAG CAACCACACA
    GTCATCAATA CAACATATGG TTACCGTGAC AACCCCACCA GTCAAGACGA GAAGTGCATG
    AGCAGTCTTA AACTGGGAAT GACTATGTGG GAGGGGCCAG CCAACAGCCA ATCACGTAGC
    CGTTCTGCTA CTTACAGCTC TGACGAATCA GAGGGTGAGG AGATGTGGGA GGAGCTACTG
    GAGCTTCGTA AAAGACACTT GGTGGAGGTC CAGAATCTAC AGATGAGTCA AAAACAAGAA
    ATGGAGGAGT TGTACAGACG GAAGGGTAAA GCCCTACCAC CTGGGATCGT CCCTCCAGCC
    GCCATGCTGA ACCACCGCCA ACGCCGCCTC TCCAAGACAG GAAACTATCC TCTGGCACGC
    CGAAGCAGCC TCCAGAGACT AGATCTGCCG CCCCCTGCAG GGATCATGAG GAAGGGCTCG
    GTCAGCGGTA GCAGTAGTGG ATCTCAAGAC CGGATCCTGA AAGGAGTGAC GTTTGCTCCT
    GGTCACAGCT GCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023812933.1
    CDS909..5366
    Translation

    Target ORF information:

    RefSeq Version XM_023957165.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes serine/threonine-protein kinase WNK1 (LOC101162572), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023957165.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGCACT CCGCTGAAGA TGAAGAAGAG GAGAACTCTG CTCTTCATCT CAAGGACTCC 
    GACCAATCAG AGGCTTCCGA TCCACACACA GGTCATGATG TCAGCTCAGA CTCTGCCGAT
    GAAATGGGCA TCAATCTCTC AGAATTAAAC AGTGAGATAT TACCATGGCA ACGCACCCAG
    GAGGAGGAGC ACGACGAAGA GGAAATGAAC GCTGTGGCGT CGTCGCCTGA TGGGCGGTTC
    CTGAAGTTCA ACATCGAGAT CGGGAGGGGC TCCTTCAAAA CCGTTTACAA AGGGCTGGAC
    ACGGAGACCA CAGTGGAGGT GGCCTGGTGC GAGCTCCAGA CCCACCGGCT GAGCAGGGCC
    GAGCGTCAGC GTTTCAGCGA GGAGGTGGAG ATGCTCAAAG CGCTTCAGCA CCCCAACATC
    GTCCGTTTCT TCGATTCCTG GAAGTCCGCG CTGAAGGGCC ACAAATGCAC CATCCTGGTG
    ACGGAGCTCA TGACGTCTGG AACGCTGAAG ATGTACCTGC GCAGGTTCCG TCAGATGAAG
    CTGAAGCTGC TGCAGCGCTG GAGCTTCCAG GTCCTGCGGG GGCTTCACTT CCTGCACTCA
    CGTTCCCCCC CCATCATACA CCGTGACCTC AAATGTGACA ACATCTTCAT CACGGGTCCC
    TCCGCCTCCG TCAAAATCGG AGACCTGGGC CTGGCAACGC TGAAGAAGGC CTCGTTTGCT
    AAGAGCGTGA TCGGAACGCC GGAGTTCATG GCTCCAGAGA TGTACGAGGA GAAGTACGAC
    GAGGCTGTGG ACGTGTACGC CTTCGGCATG TGCATGCTGG AGATGGCTAC GTCCGAGTAT
    CCGTACCACG AGTGTCACAG CGCTGCCCAG ATCTACCGCA AGGTCACCAA TGGAATCAAA
    CCGGACAGTT TCTTCAAAGT GGAAGTTCCA GAACTGAAAG AAATCATTGA AGGCTGCATT
    CGGACCAGGA GCAGTGAAAG GTTTACAGTC CAGGACCTCT TGGAGCACAG CTTCTTTCAG
    GAGAACACCG GCGTCCATGT GGAGCTGGCA GAGGAAGACG ACGGCTCCAA GGCGGCGCTG
    AAGCTGTGGC TCCGGATGGA CGACAAGGAG AAGCTCCGGG GGAAGTATAA AGACAACGAC
    GCCATCGAGT TCCTGTACGA ACTCTACAAG GATGTGCCAG AGGAGGTCGC CCAGGAGATG
    GTCATTTTGG GATTTGTGTT CAAAGCAGAC TACAAGGTGA TTGCCAAAGC CATTCGGCAT
    CGAGTGACAG CCATAAAGTA TCAGCGGGAA AAACGGCGCC TCCTGCTGGA GGAAGGAGTG
    AACAGTCAGA AGCAAACTGA CATCGAGGAA GAGCTTCATC TTCATCAACG AGCTGAGCTG
    TCCAATCAGA CGCTCCCAGT TTCAACAGGA GCCAGAGGAG CGGCCAGTCA GGGGGACTCT
    GCTGCATCCA CACAAGCTCC GCCCCTGACT GCAACAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCTCT GACTGCAACA GCACAAGCTC CGCCCCTGAC TGCATCAGCA
    CAAGCTCCGC CCCTGACTGC AACAGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCAGC ACAAGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGACTGCAT CCACACACGC TCCTTCTCTA ACTGCATCGG CACAAGCTCC GCCCCTGACT
    GCATCAGCAC AAGCTCCGCC CCTGACTGCA TCCACACACG CTCCGCCCCT GACTGCATCA
    GCACAAGCTC CGCCCCTGAT TGCATCCACA CACTCTCCTT CCCTAACTGC ATCGGCACAA
    GCTCCACCCC TGACTGCATC CACACACGCT CCTTCCCTAA CTGCATCGGC ACAAGCTCCG
    CCCCTGACTG CATCGGCACA AACTCCGCCC CTGACTGCAT CGGCACAAGC TCCGCCCCTG
    ACTGCATCAG CACAAGCTCC GCCCCTGACT GCATCCACAC ACGCTCCGCC CCTGACTTTA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CAAGCTCCGC CCAGCAGCCG GGGGGATTCC GGGATCAACA CTGTCTCCAG CAGAGCTGAT
    GGCGACACCG AGGAGGACGG TGCGATCCGA CTAGTCTCCC ACCCCCCCAC TGCCTCAGAC
    CATGTGATTG AAACCACGCC TCTTCTTGTT CCTAACCCCA CTCCTCCATC AGTACCTCCA
    CAGACGGAGG TTCCTTCTGT TTTGACCCGC ACAGCTTCCA AAGCTCCGCC CTTTCCCATA
    CTACGCTTTC CTAAAAGCAT TGCTGTGTCC CAGGCTGCAG TAAAGCCCCC CCCTGAGCCC
    TCCTATGATT TCTCTTCACC TGTCGACAGC TGCTCTTCTG ACTTCACCTC GGGCTTGAGT
    GATGGCAATG ACGGCCAATC AGAGAGGAGC AGGATGGAGG CAACGATGAG GGAAGCAACC
    AAACAGTTTA GAAGGAGAGC CAGAGCTCGT CTCCGAATCA TAGGGGTGTC TGACAAGGTG
    GACCGAGTGG TGGAGTGTCA GCTGCAGACT CACAACAACA AGATGGTCAC GTTTAAGTTT
    GACCTGGATG GAGACAATCC CGAGGACATC GCATCTGTGA TGATTCACAG AGATTTTATC
    TTACCGTCTG AGCGAGAAGG ATTTATCCTC CGCATGTATG ACATCATCCA GAGGGCGGAG
    TCTATGATAC ACCAGCATCA TCCTGCAGTC TTGTACCAGC CTTCTCAGCT GATGACCTCT
    GCTCCCCAGT CAAACTGCCT CTGCTTTCAA CCTTTTCAGC CAAAGTCAGC AGTGACTAAC
    CTGTCCAGGA CTTTGTCTTC CTCCTCCTTA CCTGACATAG ATGAAAGTGA CACCACCCCC
    TCAAAGGCAA TTGACTTCAG CATTGACCCT GATGCTGTTC CCCCCGTGAG GCCGTTGAGG
    TCACAGTCCT TCCAGACGTC ATCAGCCTCT TCCTATGAGC CCCCCCTTTA CCCTTACCAT
    TACCCCCATC CTGAATCTAC CACTCCCCCC CTCTCTCAGA ATCTGCTTCC TTCTCATCTT
    TACTCCACCT ATACAGCCTC TTTCTCCACG CCCAGCTCCG CCCCCCAGAT TCCACGAGTC
    CACAGCAACC CCTCCCTCTT TACCTCCACA CCATCATCTT TAGCCCCTCC CTCTCCTAGG
    TGGCCACCCC CTGACCAGCC TCTCTTCTCT TTGGCCAATG TACTGTCTGT GGCCATGAGT
    GTGGCCCACA GCCTCATGCC CCCACCCGGA CATCCGAGTC AAGGCTTTCA ACACCAGGCT
    CCTCCCCCGC CTTTCCCCCA GTCCACGTTC TCACCCCCCA TGTCTCCATC TCTAAAACGG
    AACCTTCAGA CTTTGACAAA CACCTCCACC GCTGAACCCC ACCAACCCCC CCAGGCCTAT
    CACACAGGCT TGGAGCCAGA AGTCCAAAAG ACAGTGATGG ACGGCGGAGC TGCAGATCAG
    TGTCCCGGTC CTGCCCGACC ACGGCTCGGC TCGGCTCCCT CCTCCCAGGG TTCAGAGACC
    TCCGTTCTTC CCCGTCAGGC CAGCCTCTCA AATCCCAGAG GAGAGTTTTC AGATTCCTCC
    ACCTCGTCCT CAGCCTCCCC TCCTCCCCCA TCGGGTTTCT ACCCCAAAGT GTCCCCCCCA
    GTGTCCCCAT CACGCTCCAT CTCTCCTACG TTGAAAGCGG GGAAAACAGC CTTCACTGTG
    GGCCGCTTCC AGGTGACCCC ATCAACACCC GTTTCTGCTG TCAGTCATCA TCAGCCACGC
    CCCTTTAGTG ATGCTTCACC CATCGCTCAC TCCCCCCCTC CTACTCAGAA GAACCAATCA
    GAGAGCAGCT CAAGAAGCAG TTCAGAAGAA GACAGCCAAT CAGAAATCAG CAACCACACA
    GTCATCAATA CAACATATGG TTACCGTGAC AACCCCACCA GTCAAGACGA GAAGTGCATG
    AGCAGTCTTA AACTGGGAAT GACTATGTGG GAGGGGCCAG CCAACAGCCA ATCACGTAGC
    CGTTCTGCTA CTTACAGCTC TGACGAATCA GAGGGTGAGG AGATGTGGGA GGAGCTACTG
    GAGCTTCGTA AAAGACACTT GGTGGAGGTC CAGAATCTAC AGATGAGTCA AAAACAAGAA
    ATGGAGGAGT TGTACAGACG GAAGGGTAAA GCCCTACCAC CTGGGATCGT CCCTCCAGCC
    GCCATGCTGA ACCACCGCCA ACGCCGCCTC TCCAAGACAG GAAACTATCC TCTGGCACGC
    CGAAGCAGCC TCCAGAGACT AGATCTGCCG CCCCCTGCAG GGATCATGAG GAAGGGCTCG
    GTCAGCGGTA GCAGTAGTGG ATCTCAAGAC CGGATCCTGA AAGGAGTGAC GTTTGCTCCT
    GGTCACAGCT GCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa117519
    Clone ID Related Accession (Same CDS sequence) XM_023957166.1
    Accession Version XM_023957166.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1518537600000
    Organism Oryzias latipes(Japanese medaka)
    Product serine/threonine-protein kinase WNK1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019866.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCTCAAAG CGCTTCAGCA CCCCAACATC GTCCGTTTCT TCGATTCCTG GAAGTCCGCG 
    CTGAAGGGCC ACAAATGCAC CATCCTGGTG ACGGAGCTCA TGACGTCTGG AACGCTGAAG
    ATGTACCTGC GCAGGTTCCG TCAGATGAAG CTGAAGCTGC TGCAGCGCTG GAGCTTCCAG
    GTCCTGCGGG GGCTTCACTT CCTGCACTCA CGTTCCCCCC CCATCATACA CCGTGACCTC
    AAATGTGACA ACATCTTCAT CACGGGTCCC TCCGCCTCCG TCAAAATCGG AGACCTGGGC
    CTGGCAACGC TGAAGAAGGC CTCGTTTGCT AAGAGCGTGA TCGGAACGCC GGAGTTCATG
    GCTCCAGAGA TGTACGAGGA GAAGTACGAC GAGGCTGTGG ACGTGTACGC CTTCGGCATG
    TGCATGCTGG AGATGGCTAC GTCCGAGTAT CCGTACCACG AGTGTCACAG CGCTGCCCAG
    ATCTACCGCA AGGTCACCAA TGGAATCAAA CCGGACAGTT TCTTCAAAGT GGAAGTTCCA
    GAACTGAAAG AAATCATTGA AGGCTGCATT CGGACCAGGA GCAGTGAAAG GTTTACAGTC
    CAGGACCTCT TGGAGCACAG CTTCTTTCAG GAGAACACCG GCGTCCATGT GGAGCTGGCA
    GAGGAAGACG ACGGCTCCAA GGCGGCGCTG AAGCTGTGGC TCCGGATGGA CGACAAGGAG
    AAGCTCCGGG GGAAGTATAA AGACAACGAC GCCATCGAGT TCCTGTACGA ACTCTACAAG
    GATGTGCCAG AGGAGGTCGC CCAGGAGATG GTCATTTTGG GATTTGTGTT CAAAGCAGAC
    TACAAGGTGA TTGCCAAAGC CATTCGGCAT CGAGTGACAG CCATAAAGTA TCAGCGGGAA
    AAACGGCGCC TCCTGCTGGA GGAAGGAGTG AACAGTCAGA AGCAAACTGA CATCGAGGAA
    GAGCTTCATC TTCATCAACG AGCTGAGCTG TCCAATCAGA CGCTCCCAGT TTCAACAGGA
    GCCAGAGGAG CGGCCAGTCA GGGGGACTCT GCTGCATCCA CACAAGCTCC GCCCCTGACT
    GCAACAGCAC AAGCTCCGCC CCTGACTGCA TCAGCACAAG CTCCGCCTCT GACTGCAACA
    GCACAAGCTC CGCCCCTGAC TGCATCAGCA CAAGCTCCGC CCCTGACTGC AACAGCACAA
    GCTCCGCCCC TGACTGCATC AGCACAAGCT CCGCCCCTGA CTGCATCAGC ACAAGCTCCG
    CCCCTGACTG CATCAGCACA AGCTCCGCCC CTGACTGCAT CCACACACGC TCCTTCTCTA
    ACTGCATCGG CACAAGCTCC GCCCCTGACT GCATCAGCAC AAGCTCCGCC CCTGACTGCA
    TCCACACACG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAT TGCATCCACA
    CACTCTCCTT CCCTAACTGC ATCGGCACAA GCTCCACCCC TGACTGCATC CACACACGCT
    CCTTCCCTAA CTGCATCGGC ACAAGCTCCG CCCCTGACTG CATCGGCACA AACTCCGCCC
    CTGACTGCAT CGGCACAAGC TCCGCCCCTG ACTGCATCAG CACAAGCTCC GCCCCTGACT
    GCATCCACAC ACGCTCCGCC CCTGACTTTA TCAGCACAAG CTCCGCCCCT GACTGCATCA
    GCACAAGCTC CGCCCCTGAC TGCATCCACA CAAGCTCCGC CCAGCAGCCG GGGGGATTCC
    GGGATCAACA CTGTCTCCAG CAGAGCTGAT GGCGACACCG AGGAGGACGG TGCGATCCGA
    CTAGTCTCCC ACCCCCCCAC TGCCTCAGAC CATGTGATTG AAACCACGCC TCTTCTTGTT
    CCTAACCCCA CTCCTCCATC AGTACCTCCA CAGACGGAGG TTCCTTCTGT TTTGACCCGC
    ACAGCTTCCA AAGCTCCGCC CTTTCCCATA CTACGCTTTC CTAAAAGCAT TGCTGTGTCC
    CAGGCTGCAG TAAAGCCCCC CCCTGAGCCC TCCTATGATT TCTCTTCACC TGTCGACAGC
    TGCTCTTCTG ACTTCACCTC GGGCTTGAGT GATGGCAATG ACGGCCAATC AGAGAGGAGC
    AGGATGGAGG CAACGATGAG GGAAGCAACC AAACAGTTTA GAAGGAGAGC CAGAGCTCGT
    CTCCGAATCA TAGGGGTGTC TGACAAGGTG GACCGAGTGG TGGAGTGTCA GCTGCAGACT
    CACAACAACA AGATGGTCAC GTTTAAGTTT GACCTGGATG GAGACAATCC CGAGGACATC
    GCATCTGTGA TGATTCACAG AGATTTTATC TTACCGTCTG AGCGAGAAGG ATTTATCCTC
    CGCATGTATG ACATCATCCA GAGGGCGGAG TCTATGATAC ACCAGCATCA TCCTGCAGTC
    TTGTACCAGC CTTCTCAGCT GATGACCTCT GCTCCCCAGT CAAACTGCCT CTGCTTTCAA
    CCTTTTCAGC CAAAGTCAGC AGTGACTAAC CTGTCCAGGA CTTTGTCTTC CTCCTCCTTA
    CCTGACATAG ATGAAAGTGA CACCACCCCC TCAAAGGCAA TTGACTTCAG CATTGACCCT
    GATGCTGTTC CCCCCGTGAG GCCGTTGAGG TCACAGTCCT TCCAGACGTC ATCAGCCTCT
    TCCTATGAGC CCCCCCTTTA CCCTTACCAT TACCCCCATC CTGAATCTAC CACTCCCCCC
    CTCTCTCAGA ATCTGCTTCC TTCTCATCTT TACTCCACCT ATACAGCCTC TTTCTCCACG
    CCCAGCTCCG CCCCCCAGAT TCCACGAGTC CACAGCAACC CCTCCCTCTT TACCTCCACA
    CCATCATCTT TAGCCCCTCC CTCTCCTAGG TGGCCACCCC CTGACCAGCC TCTCTTCTCT
    TTGGCCAATG TACTGTCTGT GGCCATGAGT GTGGCCCACA GCCTCATGCC CCCACCCGGA
    CATCCGAGTC AAGGCTTTCA ACACCAGGCT CCTCCCCCGC CTTTCCCCCA GTCCACGTTC
    TCACCCCCCA TGTCTCCATC TCTAAAACGG AACCTTCAGA CTTTGACAAA CACCTCCACC
    GCTGAACCCC ACCAACCCCC CCAGGCCTAT CACACAGGCT TGGAGCCAGA AGTCCAAAAG
    ACAGTGATGG ACGGCGGAGC TGCAGATCAG TGTCCCGGTC CTGCCCGACC ACGGCTCGGC
    TCGGCTCCCT CCTCCCAGGG TTCAGAGACC TCCGTTCTTC CCCGTCAGGC CAGCCTCTCA
    AATCCCAGAG GAGAGTTTTC AGATTCCTCC ACCTCGTCCT CAGCCTCCCC TCCTCCCCCA
    TCGGGTTTCT ACCCCAAAGT GTCCCCCCCA GTGTCCCCAT CACGCTCCAT CTCTCCTACG
    TTGAAAGCGG GGAAAACAGC CTTCACTGTG GGCCGCTTCC AGGTGACCCC ATCAACACCC
    GTTTCTGCTG TCAGTCATCA TCAGCCACGC CCCTTTAGTG ATGCTTCACC CATCGCTCAC
    TCCCCCCCTC CTACTCAGAA GAACCAATCA GAGAGCAGCT CAAGAAGCAG TTCAGAAGAA
    GACAGCCAAT CAGAAATCAG CAACCACACA GTCATCAATA CAACATATGG TTACCGTGAC
    AACCCCACCA GTCAAGACGA GAAGTGCATG AGCAGTCTTA AACTGGGAAT GACTATGTGG
    GAGGGGCCAG CCAACAGCCA ATCACGTAGC CGTTCTGCTA CTTACAGCTC TGACGAATCA
    GAGGGTGAGG AGATGTGGGA GGAGCTACTG GAGCTTCGTA AAAGACACTT GGTGGAGGTC
    CAGAATCTAC AGATGAGTCA AAAACAAGAA ATGGAGGAGT TGTACAGACG GAAGGGTAAA
    GCCCTACCAC CTGGGATCGT CCCTCCAGCC GCCATGCTGA ACCACCGCCA ACGCCGCCTC
    TCCAAGACAG GAAACTATCC TCTGGCACGC CGAAGCAGCC TCCAGAGACT AGATCTGCCG
    CCCCCTGCAG GGATCATGAG GAAGGGCTCG GTCAGCGGTA GCAGTAGTGG ATCTCAAGAC
    CGGATCCTGA AAGGAGTGAC GTTTGCTCCT GGTCACAGCT GCATGGTGAG CAACAGAGGA
    CCAGAACCAG CTCCATACCC GGATCTCTGT AGAAGTCAGG ACCAGAATCA CAGTCAGGAC
    CAGAATCACA GTCAGGACCA GGACTACGAC CAAATTCACA GTCAGGACGA GGTTCAGGTC
    CAGGTCCAGG ACCAGGGGCC TCATTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023812934.1
    CDS918..5147
    Translation

    Target ORF information:

    RefSeq Version XM_023957166.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes serine/threonine-protein kinase WNK1 (LOC101162572), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023957166.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCTCAAAG CGCTTCAGCA CCCCAACATC GTCCGTTTCT TCGATTCCTG GAAGTCCGCG 
    CTGAAGGGCC ACAAATGCAC CATCCTGGTG ACGGAGCTCA TGACGTCTGG AACGCTGAAG
    ATGTACCTGC GCAGGTTCCG TCAGATGAAG CTGAAGCTGC TGCAGCGCTG GAGCTTCCAG
    GTCCTGCGGG GGCTTCACTT CCTGCACTCA CGTTCCCCCC CCATCATACA CCGTGACCTC
    AAATGTGACA ACATCTTCAT CACGGGTCCC TCCGCCTCCG TCAAAATCGG AGACCTGGGC
    CTGGCAACGC TGAAGAAGGC CTCGTTTGCT AAGAGCGTGA TCGGAACGCC GGAGTTCATG
    GCTCCAGAGA TGTACGAGGA GAAGTACGAC GAGGCTGTGG ACGTGTACGC CTTCGGCATG
    TGCATGCTGG AGATGGCTAC GTCCGAGTAT CCGTACCACG AGTGTCACAG CGCTGCCCAG
    ATCTACCGCA AGGTCACCAA TGGAATCAAA CCGGACAGTT TCTTCAAAGT GGAAGTTCCA
    GAACTGAAAG AAATCATTGA AGGCTGCATT CGGACCAGGA GCAGTGAAAG GTTTACAGTC
    CAGGACCTCT TGGAGCACAG CTTCTTTCAG GAGAACACCG GCGTCCATGT GGAGCTGGCA
    GAGGAAGACG ACGGCTCCAA GGCGGCGCTG AAGCTGTGGC TCCGGATGGA CGACAAGGAG
    AAGCTCCGGG GGAAGTATAA AGACAACGAC GCCATCGAGT TCCTGTACGA ACTCTACAAG
    GATGTGCCAG AGGAGGTCGC CCAGGAGATG GTCATTTTGG GATTTGTGTT CAAAGCAGAC
    TACAAGGTGA TTGCCAAAGC CATTCGGCAT CGAGTGACAG CCATAAAGTA TCAGCGGGAA
    AAACGGCGCC TCCTGCTGGA GGAAGGAGTG AACAGTCAGA AGCAAACTGA CATCGAGGAA
    GAGCTTCATC TTCATCAACG AGCTGAGCTG TCCAATCAGA CGCTCCCAGT TTCAACAGGA
    GCCAGAGGAG CGGCCAGTCA GGGGGACTCT GCTGCATCCA CACAAGCTCC GCCCCTGACT
    GCAACAGCAC AAGCTCCGCC CCTGACTGCA TCAGCACAAG CTCCGCCTCT GACTGCAACA
    GCACAAGCTC CGCCCCTGAC TGCATCAGCA CAAGCTCCGC CCCTGACTGC AACAGCACAA
    GCTCCGCCCC TGACTGCATC AGCACAAGCT CCGCCCCTGA CTGCATCAGC ACAAGCTCCG
    CCCCTGACTG CATCAGCACA AGCTCCGCCC CTGACTGCAT CCACACACGC TCCTTCTCTA
    ACTGCATCGG CACAAGCTCC GCCCCTGACT GCATCAGCAC AAGCTCCGCC CCTGACTGCA
    TCCACACACG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAT TGCATCCACA
    CACTCTCCTT CCCTAACTGC ATCGGCACAA GCTCCACCCC TGACTGCATC CACACACGCT
    CCTTCCCTAA CTGCATCGGC ACAAGCTCCG CCCCTGACTG CATCGGCACA AACTCCGCCC
    CTGACTGCAT CGGCACAAGC TCCGCCCCTG ACTGCATCAG CACAAGCTCC GCCCCTGACT
    GCATCCACAC ACGCTCCGCC CCTGACTTTA TCAGCACAAG CTCCGCCCCT GACTGCATCA
    GCACAAGCTC CGCCCCTGAC TGCATCCACA CAAGCTCCGC CCAGCAGCCG GGGGGATTCC
    GGGATCAACA CTGTCTCCAG CAGAGCTGAT GGCGACACCG AGGAGGACGG TGCGATCCGA
    CTAGTCTCCC ACCCCCCCAC TGCCTCAGAC CATGTGATTG AAACCACGCC TCTTCTTGTT
    CCTAACCCCA CTCCTCCATC AGTACCTCCA CAGACGGAGG TTCCTTCTGT TTTGACCCGC
    ACAGCTTCCA AAGCTCCGCC CTTTCCCATA CTACGCTTTC CTAAAAGCAT TGCTGTGTCC
    CAGGCTGCAG TAAAGCCCCC CCCTGAGCCC TCCTATGATT TCTCTTCACC TGTCGACAGC
    TGCTCTTCTG ACTTCACCTC GGGCTTGAGT GATGGCAATG ACGGCCAATC AGAGAGGAGC
    AGGATGGAGG CAACGATGAG GGAAGCAACC AAACAGTTTA GAAGGAGAGC CAGAGCTCGT
    CTCCGAATCA TAGGGGTGTC TGACAAGGTG GACCGAGTGG TGGAGTGTCA GCTGCAGACT
    CACAACAACA AGATGGTCAC GTTTAAGTTT GACCTGGATG GAGACAATCC CGAGGACATC
    GCATCTGTGA TGATTCACAG AGATTTTATC TTACCGTCTG AGCGAGAAGG ATTTATCCTC
    CGCATGTATG ACATCATCCA GAGGGCGGAG TCTATGATAC ACCAGCATCA TCCTGCAGTC
    TTGTACCAGC CTTCTCAGCT GATGACCTCT GCTCCCCAGT CAAACTGCCT CTGCTTTCAA
    CCTTTTCAGC CAAAGTCAGC AGTGACTAAC CTGTCCAGGA CTTTGTCTTC CTCCTCCTTA
    CCTGACATAG ATGAAAGTGA CACCACCCCC TCAAAGGCAA TTGACTTCAG CATTGACCCT
    GATGCTGTTC CCCCCGTGAG GCCGTTGAGG TCACAGTCCT TCCAGACGTC ATCAGCCTCT
    TCCTATGAGC CCCCCCTTTA CCCTTACCAT TACCCCCATC CTGAATCTAC CACTCCCCCC
    CTCTCTCAGA ATCTGCTTCC TTCTCATCTT TACTCCACCT ATACAGCCTC TTTCTCCACG
    CCCAGCTCCG CCCCCCAGAT TCCACGAGTC CACAGCAACC CCTCCCTCTT TACCTCCACA
    CCATCATCTT TAGCCCCTCC CTCTCCTAGG TGGCCACCCC CTGACCAGCC TCTCTTCTCT
    TTGGCCAATG TACTGTCTGT GGCCATGAGT GTGGCCCACA GCCTCATGCC CCCACCCGGA
    CATCCGAGTC AAGGCTTTCA ACACCAGGCT CCTCCCCCGC CTTTCCCCCA GTCCACGTTC
    TCACCCCCCA TGTCTCCATC TCTAAAACGG AACCTTCAGA CTTTGACAAA CACCTCCACC
    GCTGAACCCC ACCAACCCCC CCAGGCCTAT CACACAGGCT TGGAGCCAGA AGTCCAAAAG
    ACAGTGATGG ACGGCGGAGC TGCAGATCAG TGTCCCGGTC CTGCCCGACC ACGGCTCGGC
    TCGGCTCCCT CCTCCCAGGG TTCAGAGACC TCCGTTCTTC CCCGTCAGGC CAGCCTCTCA
    AATCCCAGAG GAGAGTTTTC AGATTCCTCC ACCTCGTCCT CAGCCTCCCC TCCTCCCCCA
    TCGGGTTTCT ACCCCAAAGT GTCCCCCCCA GTGTCCCCAT CACGCTCCAT CTCTCCTACG
    TTGAAAGCGG GGAAAACAGC CTTCACTGTG GGCCGCTTCC AGGTGACCCC ATCAACACCC
    GTTTCTGCTG TCAGTCATCA TCAGCCACGC CCCTTTAGTG ATGCTTCACC CATCGCTCAC
    TCCCCCCCTC CTACTCAGAA GAACCAATCA GAGAGCAGCT CAAGAAGCAG TTCAGAAGAA
    GACAGCCAAT CAGAAATCAG CAACCACACA GTCATCAATA CAACATATGG TTACCGTGAC
    AACCCCACCA GTCAAGACGA GAAGTGCATG AGCAGTCTTA AACTGGGAAT GACTATGTGG
    GAGGGGCCAG CCAACAGCCA ATCACGTAGC CGTTCTGCTA CTTACAGCTC TGACGAATCA
    GAGGGTGAGG AGATGTGGGA GGAGCTACTG GAGCTTCGTA AAAGACACTT GGTGGAGGTC
    CAGAATCTAC AGATGAGTCA AAAACAAGAA ATGGAGGAGT TGTACAGACG GAAGGGTAAA
    GCCCTACCAC CTGGGATCGT CCCTCCAGCC GCCATGCTGA ACCACCGCCA ACGCCGCCTC
    TCCAAGACAG GAAACTATCC TCTGGCACGC CGAAGCAGCC TCCAGAGACT AGATCTGCCG
    CCCCCTGCAG GGATCATGAG GAAGGGCTCG GTCAGCGGTA GCAGTAGTGG ATCTCAAGAC
    CGGATCCTGA AAGGAGTGAC GTTTGCTCCT GGTCACAGCT GCATGGTGAG CAACAGAGGA
    CCAGAACCAG CTCCATACCC GGATCTCTGT AGAAGTCAGG ACCAGAATCA CAGTCAGGAC
    CAGAATCACA GTCAGGACCA GGACTACGAC CAAATTCACA GTCAGGACGA GGTTCAGGTC
    CAGGTCCAGG ACCAGGGGCC TCATTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa117520
    Clone ID Related Accession (Same CDS sequence) XM_023957167.1
    Accession Version XM_023957167.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1518537600000
    Organism Oryzias latipes(Japanese medaka)
    Product serine/threonine-protein kinase WNK1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019866.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTGCACT CCGCTGAAGA TGAAGAAGAG GAGAACTCTG CTCTTCATCT CAAGGACTCC 
    GACCAATCAG AGGCTTCCGA TCCACACACA GGTCATGATG TCAGCTCAGA CTCTGCCGAT
    GAAATGGGCA TCAATCTCTC AGAATTAAAC AGTGAGATAT TACCATGGCA ACGCACCCAG
    GAGGAGGAGC ACGACGAAGA GGAAATGAAC GCTGTGGCGT CGTCGCCTGA TGGGCGGTTC
    CTGAAGTTCA ACATCGAGAT CGGGAGGGGC TCCTTCAAAA CCGTTTACAA AGGGCTGGAC
    ACGGAGACCA CAGTGGAGGT GGCCTGGTGC GAGCTCCAGA CCCACCGGCT GAGCAGGGCC
    GAGCGTCAGC GTTTCAGCGA GGAGGTGGAG ATGCTCAAAG CGCTTCAGCA CCCCAACATC
    GTCCGTTTCT TCGATTCCTG GAAGTCCGCG CTGAAGGGCC ACAAATGCAC CATCCTGGTG
    ACGGAGCTCA TGACGTCTGG AACGCTGAAG ATGTACCTGC GCAGGTTCCG TCAGATGAAG
    CTGAAGCTGC TGCAGCGCTG GAGCTTCCAG GTCCTGCGGG GGCTTCACTT CCTGCACTCA
    CGTTCCCCCC CCATCATACA CCGTGACCTC AAATGTGACA ACATCTTCAT CACGGGTCCC
    TCCGCCTCCG TCAAAATCGG AGACCTGGGC CTGGCAACGC TGAAGAAGGC CTCGTTTGCT
    AAGAGCGTGA TCGGAACGCC GGAGTTCATG GCTCCAGAGA TGTACGAGGA GAAGTACGAC
    GAGGCTGTGG ACGTGTACGC CTTCGGCATG TGCATGCTGG AGATGGCTAC GTCCGAGTAT
    CCGTACCACG AGTGTCACAG CGCTGCCCAG ATCTACCGCA AGGTCACCAA TGGAATCAAA
    CCGGACAGTT TCTTCAAAGT GGAAGTTCCA GAACTGAAAG AAATCATTGA AGGCTGCATT
    CGGACCAGGA GCAGTGAAAG GTTTACAGTC CAGGACCTCT TGGAGCACAG CTTCTTTCAG
    GAGAACACCG GCGTCCATGT GGAGCTGGCA GAGGAAGACG ACGGCTCCAA GGCGGCGCTG
    AAGCTGTGGC TCCGGATGGA CGACAAGGAG AAGCTCCGGG GGAAGTATAA AGACAACGAC
    GCCATCGAGT TCCTGTACGA ACTCTACAAG GATGTGCCAG AGGAGGTCGC CCAGGAGATG
    GTCATTTTGG GATTTGTGTT CAAAGCAGAC TACAAGGTGA TTGCCAAAGC CATTCGGCAT
    CGAGTGACAG CCATAAAGTA TCAGCGGGAA AAACGGCGCC TCCTGCTGGA GGAAGGAGTG
    AACAGTCAGA AGCAAACTGA CATCGAGGAA GAGCTTCATC TTCATCAACG AGCTGAGCTG
    TCCAATCAGA CGCTCCCAGT TTCAACAGGA GCCAGAGGAG CGGCCAGTCA GGGGGACTCT
    GCTGCATCCA CACAAGCTCC GCCCCTGACT GCAACAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCTCT GACTGCAACA GCACAAGCTC CGCCCCTGAC TGCATCAGCA
    CAAGCTCCGC CCCTGACTGC AACAGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCAGC ACAAGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGACTGCAT CCACACACGC TCCTTCTCTA ACTGCATCGG CACAAGCTCC GCCCCTGACT
    GCATCAGCAC AAGCTCCGCC CCTGACTGCA TCCACACACG CTCCGCCCCT GACTGCATCA
    GCACAAGCTC CGCCCCTGAT TGCATCCACA CACTCTCCTT CCCTAACTGC ATCGGCACAA
    GCTCCACCCC TGACTGCATC CACACACGCT CCTTCCCTAA CTGCATCGGC ACAAGCTCCG
    CCCCTGACTG CATCGGCACA AACTCCGCCC CTGACTGCAT CGGCACAAGC TCCGCCCCTG
    ACTGCATCAG CACAAGCTCC GCCCCTGACT GCATCCACAC ACGCTCCGCC CCTGACTTTA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CAAGCTCCGC CCAGCAGCCG GGGGGATTCC GGGATCAACA CTGTCTCCAG CAGAGCTGAT
    GGCGACACCG AGGAGGACGG TGCGATCCGA CTAGTCTCCC ACCCCCCCAC TGCCTCAGAC
    CATGTGATTG AAACCACGCC TCTTCTTGTT CCTAACCCCA CTCCTCCATC AGTACCTCCA
    CAGACGGAGG TTCCTTCTGT TTTGACCCGC ACAGCTTCCA AAGCTCCGCC CTTTCCCATA
    CTACGCTTTC CTAAAAGCAT TGCTGTGTCC CAGGCTGCAG TAAAGCCCCC CCCTGAGCCC
    TCCTATGATT TCTCTTCACC TGTCGACAGC TGCTCTTCTG ACTTCACCTC GGGCTTGAGT
    GATGGCAATG ACGGCCAATC AGAGAGGAGC AGGATGGAGG CAACGATGAG GGAAGCAACC
    AAACAGTTTA GAAGGAGAGC CAGAGCTCGT CTCCGAATCA TAGGGGTGTC TGACAAGGTG
    GACCGAGTGG TGGAGTGTCA GCTGCAGACT CACAACAACA AGATGGTCAC GTTTAAGTTT
    GACCTGGATG GAGACAATCC CGAGGACATC GCATCTGTGA TGATTCACAG AGATTTTATC
    TTACCGTCTG AGCGAGAAGG ATTTATCCTC CGCATGTATG ACATCATCCA GAGGGCGGAG
    TCTATGATAC ACCAGCATCA TCCTGCAGTC TTGTACCAGC CTTCTCAGCT GATGACCTCT
    GCTCCCCAGT CAAACTGCCT CTGCTTTCAA CCTTTTCAGC CAAAGTCAGC AGTGACTAAC
    CTGTCCAGGA CTTTGTCTTC CTCCTCCTTA CCTGACATAG ATGAAAGTGA CACCACCCCC
    TCAAAGGCAA TTGACTTCAG CATTGACCCT GATGCTGTTC CCCCCGTGAG GCCGTTGAGG
    TCACAGTCCT TCCAGACGTC ATCAGCCTCT TCCTATGAGC CCCCCCTTTA CCCTTACCAT
    TACCCCCATC CTGAATCTAC CACTCCCCCC CTCTCTCAGA ATCTGCTTCC TTCTCATCTT
    TACTCCACCT ATACAGCCTC TTTCTCCACG CCCAGCTCCG CCCCCCAGAT TCCACGAGTC
    CACAGCAACC CCTCCCTCTT TACCTCCACA CCATCATCTT TAGCCCCTCC CTCTCCTAGG
    TGGCCACCCC CTGACCAGCC TCTCTTCTCT TTGGCCAATG TACTGTCTGT GGCCATGAGT
    GTGGCCCACA GCCTCATGCC CCCACCCGGA CATCCGAGTC AAGGCTTTCA ACACCAGGCT
    CCTCCCCCGC CTTTCCCCCA GTCCACGTTC TCACCCCCCA TGTCTCCATC TCTAAAACGG
    AACCTTCAGA CTTTGACAAA CACCTCCACC GCTGAACCCC ACCAACCCCC CCAGGCCTAT
    CACACAGGCT TGGAGCCAGA AGTCCAAAAG ACAGTGATGG ACGGCGGAGC TGCAGATCAG
    TGTCCCGGTC CTGCCCGACC ACGGCTCGGC TCGGCTCCCT CCTCCCAGGG TTCAGAGACC
    TCCGTTCTTC CCCGTCAGGC CAGCCTCTCA AATCCCAGAG GAGAGTTTTC AGATTCCTCC
    ACCTCGTCCT CAGCCTCCCC TCCTCCCCCA TCGGGTTTCT ACCCCAAAGT GTCCCCCCCA
    GTGTCCCCAT CACGCTCCAT CTCTCCTACG TTGAAAGACA CTTGGTGGAG GTCCAGAATC
    TACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023812935.1
    CDS928..4716
    Translation

    Target ORF information:

    RefSeq Version XM_023957167.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes serine/threonine-protein kinase WNK1 (LOC101162572), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023957167.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTGCACT CCGCTGAAGA TGAAGAAGAG GAGAACTCTG CTCTTCATCT CAAGGACTCC 
    GACCAATCAG AGGCTTCCGA TCCACACACA GGTCATGATG TCAGCTCAGA CTCTGCCGAT
    GAAATGGGCA TCAATCTCTC AGAATTAAAC AGTGAGATAT TACCATGGCA ACGCACCCAG
    GAGGAGGAGC ACGACGAAGA GGAAATGAAC GCTGTGGCGT CGTCGCCTGA TGGGCGGTTC
    CTGAAGTTCA ACATCGAGAT CGGGAGGGGC TCCTTCAAAA CCGTTTACAA AGGGCTGGAC
    ACGGAGACCA CAGTGGAGGT GGCCTGGTGC GAGCTCCAGA CCCACCGGCT GAGCAGGGCC
    GAGCGTCAGC GTTTCAGCGA GGAGGTGGAG ATGCTCAAAG CGCTTCAGCA CCCCAACATC
    GTCCGTTTCT TCGATTCCTG GAAGTCCGCG CTGAAGGGCC ACAAATGCAC CATCCTGGTG
    ACGGAGCTCA TGACGTCTGG AACGCTGAAG ATGTACCTGC GCAGGTTCCG TCAGATGAAG
    CTGAAGCTGC TGCAGCGCTG GAGCTTCCAG GTCCTGCGGG GGCTTCACTT CCTGCACTCA
    CGTTCCCCCC CCATCATACA CCGTGACCTC AAATGTGACA ACATCTTCAT CACGGGTCCC
    TCCGCCTCCG TCAAAATCGG AGACCTGGGC CTGGCAACGC TGAAGAAGGC CTCGTTTGCT
    AAGAGCGTGA TCGGAACGCC GGAGTTCATG GCTCCAGAGA TGTACGAGGA GAAGTACGAC
    GAGGCTGTGG ACGTGTACGC CTTCGGCATG TGCATGCTGG AGATGGCTAC GTCCGAGTAT
    CCGTACCACG AGTGTCACAG CGCTGCCCAG ATCTACCGCA AGGTCACCAA TGGAATCAAA
    CCGGACAGTT TCTTCAAAGT GGAAGTTCCA GAACTGAAAG AAATCATTGA AGGCTGCATT
    CGGACCAGGA GCAGTGAAAG GTTTACAGTC CAGGACCTCT TGGAGCACAG CTTCTTTCAG
    GAGAACACCG GCGTCCATGT GGAGCTGGCA GAGGAAGACG ACGGCTCCAA GGCGGCGCTG
    AAGCTGTGGC TCCGGATGGA CGACAAGGAG AAGCTCCGGG GGAAGTATAA AGACAACGAC
    GCCATCGAGT TCCTGTACGA ACTCTACAAG GATGTGCCAG AGGAGGTCGC CCAGGAGATG
    GTCATTTTGG GATTTGTGTT CAAAGCAGAC TACAAGGTGA TTGCCAAAGC CATTCGGCAT
    CGAGTGACAG CCATAAAGTA TCAGCGGGAA AAACGGCGCC TCCTGCTGGA GGAAGGAGTG
    AACAGTCAGA AGCAAACTGA CATCGAGGAA GAGCTTCATC TTCATCAACG AGCTGAGCTG
    TCCAATCAGA CGCTCCCAGT TTCAACAGGA GCCAGAGGAG CGGCCAGTCA GGGGGACTCT
    GCTGCATCCA CACAAGCTCC GCCCCTGACT GCAACAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCTCT GACTGCAACA GCACAAGCTC CGCCCCTGAC TGCATCAGCA
    CAAGCTCCGC CCCTGACTGC AACAGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCAGC ACAAGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGACTGCAT CCACACACGC TCCTTCTCTA ACTGCATCGG CACAAGCTCC GCCCCTGACT
    GCATCAGCAC AAGCTCCGCC CCTGACTGCA TCCACACACG CTCCGCCCCT GACTGCATCA
    GCACAAGCTC CGCCCCTGAT TGCATCCACA CACTCTCCTT CCCTAACTGC ATCGGCACAA
    GCTCCACCCC TGACTGCATC CACACACGCT CCTTCCCTAA CTGCATCGGC ACAAGCTCCG
    CCCCTGACTG CATCGGCACA AACTCCGCCC CTGACTGCAT CGGCACAAGC TCCGCCCCTG
    ACTGCATCAG CACAAGCTCC GCCCCTGACT GCATCCACAC ACGCTCCGCC CCTGACTTTA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CAAGCTCCGC CCAGCAGCCG GGGGGATTCC GGGATCAACA CTGTCTCCAG CAGAGCTGAT
    GGCGACACCG AGGAGGACGG TGCGATCCGA CTAGTCTCCC ACCCCCCCAC TGCCTCAGAC
    CATGTGATTG AAACCACGCC TCTTCTTGTT CCTAACCCCA CTCCTCCATC AGTACCTCCA
    CAGACGGAGG TTCCTTCTGT TTTGACCCGC ACAGCTTCCA AAGCTCCGCC CTTTCCCATA
    CTACGCTTTC CTAAAAGCAT TGCTGTGTCC CAGGCTGCAG TAAAGCCCCC CCCTGAGCCC
    TCCTATGATT TCTCTTCACC TGTCGACAGC TGCTCTTCTG ACTTCACCTC GGGCTTGAGT
    GATGGCAATG ACGGCCAATC AGAGAGGAGC AGGATGGAGG CAACGATGAG GGAAGCAACC
    AAACAGTTTA GAAGGAGAGC CAGAGCTCGT CTCCGAATCA TAGGGGTGTC TGACAAGGTG
    GACCGAGTGG TGGAGTGTCA GCTGCAGACT CACAACAACA AGATGGTCAC GTTTAAGTTT
    GACCTGGATG GAGACAATCC CGAGGACATC GCATCTGTGA TGATTCACAG AGATTTTATC
    TTACCGTCTG AGCGAGAAGG ATTTATCCTC CGCATGTATG ACATCATCCA GAGGGCGGAG
    TCTATGATAC ACCAGCATCA TCCTGCAGTC TTGTACCAGC CTTCTCAGCT GATGACCTCT
    GCTCCCCAGT CAAACTGCCT CTGCTTTCAA CCTTTTCAGC CAAAGTCAGC AGTGACTAAC
    CTGTCCAGGA CTTTGTCTTC CTCCTCCTTA CCTGACATAG ATGAAAGTGA CACCACCCCC
    TCAAAGGCAA TTGACTTCAG CATTGACCCT GATGCTGTTC CCCCCGTGAG GCCGTTGAGG
    TCACAGTCCT TCCAGACGTC ATCAGCCTCT TCCTATGAGC CCCCCCTTTA CCCTTACCAT
    TACCCCCATC CTGAATCTAC CACTCCCCCC CTCTCTCAGA ATCTGCTTCC TTCTCATCTT
    TACTCCACCT ATACAGCCTC TTTCTCCACG CCCAGCTCCG CCCCCCAGAT TCCACGAGTC
    CACAGCAACC CCTCCCTCTT TACCTCCACA CCATCATCTT TAGCCCCTCC CTCTCCTAGG
    TGGCCACCCC CTGACCAGCC TCTCTTCTCT TTGGCCAATG TACTGTCTGT GGCCATGAGT
    GTGGCCCACA GCCTCATGCC CCCACCCGGA CATCCGAGTC AAGGCTTTCA ACACCAGGCT
    CCTCCCCCGC CTTTCCCCCA GTCCACGTTC TCACCCCCCA TGTCTCCATC TCTAAAACGG
    AACCTTCAGA CTTTGACAAA CACCTCCACC GCTGAACCCC ACCAACCCCC CCAGGCCTAT
    CACACAGGCT TGGAGCCAGA AGTCCAAAAG ACAGTGATGG ACGGCGGAGC TGCAGATCAG
    TGTCCCGGTC CTGCCCGACC ACGGCTCGGC TCGGCTCCCT CCTCCCAGGG TTCAGAGACC
    TCCGTTCTTC CCCGTCAGGC CAGCCTCTCA AATCCCAGAG GAGAGTTTTC AGATTCCTCC
    ACCTCGTCCT CAGCCTCCCC TCCTCCCCCA TCGGGTTTCT ACCCCAAAGT GTCCCCCCCA
    GTGTCCCCAT CACGCTCCAT CTCTCCTACG TTGAAAGACA CTTGGTGGAG GTCCAGAATC
    TACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa67999
    Clone ID Related Accession (Same CDS sequence) XM_020700646.1
    Accession Version XM_020700646.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1491408000000
    Organism Oryzias latipes(Japanese medaka)
    Product serine/threonine-protein kinase WNK4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004090421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGACGACA AGGAGAAGCT CCGGGGGAAG TATAAAGACA ACGACGCCAT CGAGTTCCTG 
    TACGAACTCT ACAAGGATGT GCCAGAGGAG GTCGCCCAGG AGATGGTCAT TTTGGGATTT
    GTGTTCAAAG CAGACTACAA GGTGATTGCC AAAGCCATTC GGCATCGAGT GACAGCCATA
    AAGTATCAGC GGGAAAAACG GCGCCTCCTG CTGGAGGAAG GAGTGAACAG TCAGAAGCAA
    ACTGACATCG AGGAAGAGCT TCATCTTCAT CAACGAGCTG AGCTGTCCAA TCAGACGCTC
    CCAGTTTCAA CAGGAGCCAG AGGAGCGGCC AGTCAGGGGG ACTCTGCTGC ATCCACACAA
    GCTCCGCCCC TGACTGCAAC AGCACAAGCT CCGCCCCTGA CTGCATCAGC ACAAGCTCCG
    CCTCTGACTG CAACAGCACA AGCTCCGCCC CTGACTGCAT CAGCACAAGC TCCGCCCCTG
    ACTGCAACAG CACAAGCTCC GCCCCTGACT GCATCAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CACGCTCCTT CTCTAACTGC ATCGGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCCAC ACACGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGATTGCAT CCACACACTC TCCTTCCCTA ACTGCATCGG CACAAGCTCC ACCCCTGACT
    GCATCCACAC ACGCTCCTTC CCTAACTGCA TCGGCACAAG CTCCGCCCCT GACTGCATCG
    GCACAAACTC CGCCCCTGAC TGCATCGGCA CAAGCTCCGC CCCTGACTGC ATCAGCACAA
    GCTCCGCCCC TGACTGCATC CACACACGCT CCGCCCCTGA CTTTATCAGC ACAAGCTCCG
    CCCCTGACTG CATCAGCACA AGCTCCGCCC CTGACTGCAT CCACACAAGC TCCGCCCAGC
    AGCCGGGGGG ATTCCGGGAT CAACACTGTC TCCAGCAGAG CTGATGGCGA CACCGAGGAG
    GACGGTGCGA TCCGACTAGT CTCCCACCCC CCCACTGCCT CAGACCATGT GATTGAAACC
    ACGCCTCTTC TTGTTCCTAA CCCCACTCCT CCATCAGTAC CTCCACAGAC GGAGGTTCCT
    TCTGTTTTGA CCCGCACAGC TTCCAAAGCT CCGCCCTTTC CCATACTACG CTTTCCTAAA
    AGCATTGCTG TGTCCCAGGC TGCAGTAAAG CCCCCCCCTG AGCCCTCCTA TGATTTCTCT
    TCACCTGTCG ACAGCTGCTC TTCTGACTTC ACCTCGGGCT TGAGTGATGG CAATGACGGC
    CAATCAGAGA GGAGCAGGAT GGAGGCAACG ATGAGGGAAG CAACCAAACA GTTTAGAAGG
    AGAGCCAGAG CTCGTCTCCG AATCATAGGG GTGTCTGACA AGGTGGACCG AGTGGTGGAG
    TGTCAGCTGC AGACTCACAA CAACAAGATG GTCACGTTTA AGTTTGACCT GGATGGAGAC
    AATCCCGAGG ACATCGCATC TGTGATGATT CACAGAGATT TTATCTTACC GTCTGAGCGA
    GAAGGATTTA TCCTCCGCAT GTATGACATC ATCCAGAGGG CGGAGTCTAT GATACACCAG
    CATCATCCTG CAGTCTTGTA CCAGCCTTCT CAGCTGATGA CCTCTGCTCC CCAGTCAAAC
    TGCCTCTGCT TTCAACCTTT TCAGCCAAAG TCAGCAGTGA CTAACCTGTC CAGGACTTTG
    TCTTCCTCCT CCTTACCTGA CATAGATGAA AGTGACACCA CCCCCTCAAA GGCAATTGAC
    TTCAGCATTG ACCCTGATGC TGTTCCCCCC GTGAGGCCGT TGAGGTCACA GTCCTTCCAG
    ACGTCATCAG CCTCTTCCTA TGAGCCCCCC CTTTACCCTT ACCATTACCC CCATCCTGAA
    TCTACCACTC CCCCCCTCTC TCAGAATCTG CTTCCTTCTC ATCTTTACTC CACCTATACA
    GCCTCTTTCT CCACGCCCAG CTCCGCCCCC CAGATTCCAC GAGTCCACAG CAACCCCTCC
    CTCTTTACCT CCACACCATC ATCTTTAGCC CCTCCCTCTC CTAGGTGGCC ACCCCCTGAC
    CAGCCTCTCT TCTCTTTGGC CAATGTACTG TCTGTGGCCA TGAGTGTGGC CCACAGCCTC
    ATGCCCCCAC CCGGACATCC GAGTCAAGGC TTTCAACACC AGGCTCCTCC CCCGCCTTTC
    CCCCAGTCCA CGTTCTCACC CCCCATGTCT CCATCTCTAA AACGGAACCT TCAGACTTTG
    ACAAACACCT CCACCGCTGA ACCCCACCAA CCCCCCCAGG CCTATCACAC AGGCTTGGAG
    CCAGAAGTCC AAAAGACAGT GATGGACGGC GGAGCTGCAG ATCAGTGTCC CGGTCCTGCC
    CGACCACGGC TCGGCTCGGC TCCCTCCTCC CAGGGTTCAG AGACCTCCGT TCTTCCCCGT
    CAGGCCAGCC TCTCAAATCC CAGAGGAGAG TTTTCAGATT CCTCCACCTC GTCCTCAGCC
    TCCCCTCCTC CCCCATCGGG TTTCTACCCC AAAGTGTCCC CCCCAGTGTC CCCATCACGC
    TCCATCTCTC CTACGTTGAA AGCGGGGAAA ACAGCCTTCA CTGTGGGCCG CTTCCAGGTG
    ACCCCATCAA CACCCGTTTC TGCTGTCAGT CATCATCAGC CACGCCCCTT TAGTGATGCT
    TCACCCATCG CTCACTCCCC CCCTCCTACT CAGAAGAACC AATCAGAGAG CAGCTCAAGA
    AGCAGTTCAG AAGAAGACAG CCAATCAGAA ATCAGCAACC ACACAGTCAT CAATACAACA
    TATGGTTACC GTGACAACCC CACCAGTCAA GACGAGAAGT GCATGAGCAG TCTTAAACTG
    GGAATGACTA TGTGGGAGGG GCCAGCCAAC AGCCAATCAC GTAGCCGTTC TGCTACTTAC
    AGCTCTGACG AATCAGAGGG TGAGGAGATG TGGGAGGAGC TACTGGAGCT TCGTAAAAGA
    CACTTGGTGG AGGTCCAGAA TCTACAGATG AGTCAAAAAC AAGAAATGGA GGAGTTGTAC
    AGACGGAAGG GTAAAGCCCT ACCACCTGGG ATCGTCCCTC CAGCCGCCAT GCTGAACCAC
    CGCCAACGCC GCCTCTCCAA GACAGGAAAC TATCCTCTGG CACGCCGAAG CAGCCTCCAG
    AGACTAGATC TGCCGCCCCC TGCAGGGATC ATGAGGAAGG GCTCGGTCAG CGGTAGCAGT
    AGTGGATCTC AAGACCGGAT CCTGAAAGGA GTGACGTTTG CTCCTGGTCA CAGCTGCATG
    GTGAGCAACA GAGGACCAGA ACCAGCTCCA TACCCGGATC TCTGTAGAAG TCAGGACCAG
    AATCACAGTC AGGACCAGAA TCACAGTCAG GACCAGGACT ACGACCAAAT TCACAGTCAG
    GACGAGGTTC AGGTCCAGGT CCAGGACCAG GGGCCTCATT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020556305.1
    CDS217..3741
    Translation

    Target ORF information:

    RefSeq Version XM_020700646.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes serine/threonine-protein kinase WNK4-like (LOC101162572), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_020700646.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGACGACA AGGAGAAGCT CCGGGGGAAG TATAAAGACA ACGACGCCAT CGAGTTCCTG 
    TACGAACTCT ACAAGGATGT GCCAGAGGAG GTCGCCCAGG AGATGGTCAT TTTGGGATTT
    GTGTTCAAAG CAGACTACAA GGTGATTGCC AAAGCCATTC GGCATCGAGT GACAGCCATA
    AAGTATCAGC GGGAAAAACG GCGCCTCCTG CTGGAGGAAG GAGTGAACAG TCAGAAGCAA
    ACTGACATCG AGGAAGAGCT TCATCTTCAT CAACGAGCTG AGCTGTCCAA TCAGACGCTC
    CCAGTTTCAA CAGGAGCCAG AGGAGCGGCC AGTCAGGGGG ACTCTGCTGC ATCCACACAA
    GCTCCGCCCC TGACTGCAAC AGCACAAGCT CCGCCCCTGA CTGCATCAGC ACAAGCTCCG
    CCTCTGACTG CAACAGCACA AGCTCCGCCC CTGACTGCAT CAGCACAAGC TCCGCCCCTG
    ACTGCAACAG CACAAGCTCC GCCCCTGACT GCATCAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CACGCTCCTT CTCTAACTGC ATCGGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCCAC ACACGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGATTGCAT CCACACACTC TCCTTCCCTA ACTGCATCGG CACAAGCTCC ACCCCTGACT
    GCATCCACAC ACGCTCCTTC CCTAACTGCA TCGGCACAAG CTCCGCCCCT GACTGCATCG
    GCACAAACTC CGCCCCTGAC TGCATCGGCA CAAGCTCCGC CCCTGACTGC ATCAGCACAA
    GCTCCGCCCC TGACTGCATC CACACACGCT CCGCCCCTGA CTTTATCAGC ACAAGCTCCG
    CCCCTGACTG CATCAGCACA AGCTCCGCCC CTGACTGCAT CCACACAAGC TCCGCCCAGC
    AGCCGGGGGG ATTCCGGGAT CAACACTGTC TCCAGCAGAG CTGATGGCGA CACCGAGGAG
    GACGGTGCGA TCCGACTAGT CTCCCACCCC CCCACTGCCT CAGACCATGT GATTGAAACC
    ACGCCTCTTC TTGTTCCTAA CCCCACTCCT CCATCAGTAC CTCCACAGAC GGAGGTTCCT
    TCTGTTTTGA CCCGCACAGC TTCCAAAGCT CCGCCCTTTC CCATACTACG CTTTCCTAAA
    AGCATTGCTG TGTCCCAGGC TGCAGTAAAG CCCCCCCCTG AGCCCTCCTA TGATTTCTCT
    TCACCTGTCG ACAGCTGCTC TTCTGACTTC ACCTCGGGCT TGAGTGATGG CAATGACGGC
    CAATCAGAGA GGAGCAGGAT GGAGGCAACG ATGAGGGAAG CAACCAAACA GTTTAGAAGG
    AGAGCCAGAG CTCGTCTCCG AATCATAGGG GTGTCTGACA AGGTGGACCG AGTGGTGGAG
    TGTCAGCTGC AGACTCACAA CAACAAGATG GTCACGTTTA AGTTTGACCT GGATGGAGAC
    AATCCCGAGG ACATCGCATC TGTGATGATT CACAGAGATT TTATCTTACC GTCTGAGCGA
    GAAGGATTTA TCCTCCGCAT GTATGACATC ATCCAGAGGG CGGAGTCTAT GATACACCAG
    CATCATCCTG CAGTCTTGTA CCAGCCTTCT CAGCTGATGA CCTCTGCTCC CCAGTCAAAC
    TGCCTCTGCT TTCAACCTTT TCAGCCAAAG TCAGCAGTGA CTAACCTGTC CAGGACTTTG
    TCTTCCTCCT CCTTACCTGA CATAGATGAA AGTGACACCA CCCCCTCAAA GGCAATTGAC
    TTCAGCATTG ACCCTGATGC TGTTCCCCCC GTGAGGCCGT TGAGGTCACA GTCCTTCCAG
    ACGTCATCAG CCTCTTCCTA TGAGCCCCCC CTTTACCCTT ACCATTACCC CCATCCTGAA
    TCTACCACTC CCCCCCTCTC TCAGAATCTG CTTCCTTCTC ATCTTTACTC CACCTATACA
    GCCTCTTTCT CCACGCCCAG CTCCGCCCCC CAGATTCCAC GAGTCCACAG CAACCCCTCC
    CTCTTTACCT CCACACCATC ATCTTTAGCC CCTCCCTCTC CTAGGTGGCC ACCCCCTGAC
    CAGCCTCTCT TCTCTTTGGC CAATGTACTG TCTGTGGCCA TGAGTGTGGC CCACAGCCTC
    ATGCCCCCAC CCGGACATCC GAGTCAAGGC TTTCAACACC AGGCTCCTCC CCCGCCTTTC
    CCCCAGTCCA CGTTCTCACC CCCCATGTCT CCATCTCTAA AACGGAACCT TCAGACTTTG
    ACAAACACCT CCACCGCTGA ACCCCACCAA CCCCCCCAGG CCTATCACAC AGGCTTGGAG
    CCAGAAGTCC AAAAGACAGT GATGGACGGC GGAGCTGCAG ATCAGTGTCC CGGTCCTGCC
    CGACCACGGC TCGGCTCGGC TCCCTCCTCC CAGGGTTCAG AGACCTCCGT TCTTCCCCGT
    CAGGCCAGCC TCTCAAATCC CAGAGGAGAG TTTTCAGATT CCTCCACCTC GTCCTCAGCC
    TCCCCTCCTC CCCCATCGGG TTTCTACCCC AAAGTGTCCC CCCCAGTGTC CCCATCACGC
    TCCATCTCTC CTACGTTGAA AGCGGGGAAA ACAGCCTTCA CTGTGGGCCG CTTCCAGGTG
    ACCCCATCAA CACCCGTTTC TGCTGTCAGT CATCATCAGC CACGCCCCTT TAGTGATGCT
    TCACCCATCG CTCACTCCCC CCCTCCTACT CAGAAGAACC AATCAGAGAG CAGCTCAAGA
    AGCAGTTCAG AAGAAGACAG CCAATCAGAA ATCAGCAACC ACACAGTCAT CAATACAACA
    TATGGTTACC GTGACAACCC CACCAGTCAA GACGAGAAGT GCATGAGCAG TCTTAAACTG
    GGAATGACTA TGTGGGAGGG GCCAGCCAAC AGCCAATCAC GTAGCCGTTC TGCTACTTAC
    AGCTCTGACG AATCAGAGGG TGAGGAGATG TGGGAGGAGC TACTGGAGCT TCGTAAAAGA
    CACTTGGTGG AGGTCCAGAA TCTACAGATG AGTCAAAAAC AAGAAATGGA GGAGTTGTAC
    AGACGGAAGG GTAAAGCCCT ACCACCTGGG ATCGTCCCTC CAGCCGCCAT GCTGAACCAC
    CGCCAACGCC GCCTCTCCAA GACAGGAAAC TATCCTCTGG CACGCCGAAG CAGCCTCCAG
    AGACTAGATC TGCCGCCCCC TGCAGGGATC ATGAGGAAGG GCTCGGTCAG CGGTAGCAGT
    AGTGGATCTC AAGACCGGAT CCTGAAAGGA GTGACGTTTG CTCCTGGTCA CAGCTGCATG
    GTGAGCAACA GAGGACCAGA ACCAGCTCCA TACCCGGATC TCTGTAGAAG TCAGGACCAG
    AATCACAGTC AGGACCAGAA TCACAGTCAG GACCAGGACT ACGACCAAAT TCACAGTCAG
    GACGAGGTTC AGGTCCAGGT CCAGGACCAG GGGCCTCATT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa68000
    Clone ID Related Accession (Same CDS sequence) XM_020700647.1
    Accession Version XM_020700647.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1491408000000
    Organism Oryzias latipes(Japanese medaka)
    Product serine/threonine-protein kinase WNK4-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004090421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGACGACA AGGAGAAGCT CCGGGGGAAG TATAAAGACA ACGACGCCAT CGAGTTCCTG 
    TACGAACTCT ACAAGGATGT GCCAGAGGAG GTCGCCCAGG AGATGGTCAT TTTGGGATTT
    GTGTTCAAAG CAGACTACAA GGTGATTGCC AAAGCCATTC GGCATCGAGT GACAGCCATA
    AAGTATCAGC GGGAAAAACG GCGCCTCCTG CTGGAGGAAG GAGTGAACAG TCAGAAGCAA
    ACTGACATCG AGGAAGAGCT TCATCTTCAT CAACGAGCTG AGCTGTCCAA TCAGACGCTC
    CCAGTTTCAA CAGGAGCCAG AGGAGCGGCC AGTCAGGGGG ACTCTGCTGC ATCCACACAA
    GCTCCGCCCC TGACTGCAAC AGCACAAGCT CCGCCCCTGA CTGCATCAGC ACAAGCTCCG
    CCTCTGACTG CAACAGCACA AGCTCCGCCC CTGACTGCAT CAGCACAAGC TCCGCCCCTG
    ACTGCAACAG CACAAGCTCC GCCCCTGACT GCATCAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CACGCTCCTT CTCTAACTGC ATCGGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCCAC ACACGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGATTGCAT CCACACACTC TCCTTCCCTA ACTGCATCGG CACAAGCTCC ACCCCTGACT
    GCATCCACAC ACGCTCCTTC CCTAACTGCA TCGGCACAAG CTCCGCCCCT GACTGCATCG
    GCACAAACTC CGCCCCTGAC TGCATCGGCA CAAGCTCCGC CCCTGACTGC ATCAGCACAA
    GCTCCGCCCC TGACTGCATC CACACACGCT CCGCCCCTGA CTTTATCAGC ACAAGCTCCG
    CCCCTGACTG CATCAGCACA AGCTCCGCCC CTGACTGCAT CCACACAAGC TCCGCCCAGC
    AGCCGGGGGG ATTCCGGGAT CAACACTGTC TCCAGCAGAG CTGATGGCGA CACCGAGGAG
    GACGGTGCGA TCCGACTAGT CTCCCACCCC CCCACTGCCT ACCATGTGAT TGAAACCACG
    CCTCTTCTTG TTCCTAACCC CACTCCTCCA TCAGTACCTC CACAGACGGA GGTTCCTTCT
    GTTTTGACCC GCACAGCTTC CAAAGCTCCG CCCTTTCCCA TACTACGCTT TCCTAAAAGC
    ATTGCTGTGT CCCAGGCTGC AGTAAAGCCC CCCCCTGAGC CCTCCTATGA TTTCTCTTCA
    CCTGTCGACA GCTGCTCTTC TGACTTCACC TCGGGCTTGA GTGATGGCAA TGACGGCCAA
    TCAGAGAGGA GCAGGATGGA GGCAACGATG AGGGAAGCAA CCAAACAGTT TAGAAGGAGA
    GCCAGAGCTC GTCTCCGAAT CATAGGGGTG TCTGACAAGG TGGACCGAGT GGTGGAGTGT
    CAGCTGCAGA CTCACAACAA CAAGATGGTC ACGTTTAAGT TTGACCTGGA TGGAGACAAT
    CCCGAGGACA TCGCATCTGT GATGATTCAC AGAGATTTTA TCTTACCGTC TGAGCGAGAA
    GGATTTATCC TCCGCATGTA TGACATCATC CAGAGGGCGG AGTCTATGAT ACACCAGCAT
    CATCCTGCAG TCTTGTACCA GCCTTCTCAG CTGATGACCT CTGCTCCCCA GTCAAACTGC
    CTCTGCTTTC AACCTTTTCA GCCAAAGTCA GCAGTGACTA ACCTGTCCAG GACTTTGTCT
    TCCTCCTCCT TACCTGACAT AGATGAAAGT GACACCACCC CCTCAAAGGC AATTGACTTC
    AGCATTGACC CTGATGCTGT TCCCCCCGTG AGGCCGTTGA GGTCACAGTC CTTCCAGACG
    TCATCAGCCT CTTCCTATGA GCCCCCCCTT TACCCTTACC ATTACCCCCA TCCTGAATCT
    ACCACTCCCC CCCTCTCTCA GAATCTGCTT CCTTCTCATC TTTACTCCAC CTATACAGCC
    TCTTTCTCCA CGCCCAGCTC CGCCCCCCAG ATTCCACGAG TCCACAGCAA CCCCTCCCTC
    TTTACCTCCA CACCATCATC TTTAGCCCCT CCCTCTCCTA GGTGGCCACC CCCTGACCAG
    CCTCTCTTCT CTTTGGCCAA TGTACTGTCT GTGGCCATGA GTGTGGCCCA CAGCCTCATG
    CCCCCACCCG GACATCCGAG TCAAGGCTTT CAACACCAGG CTCCTCCCCC GCCTTTCCCC
    CAGTCCACGT TCTCACCCCC CATGTCTCCA TCTCTAAAAC GGAACCTTCA GACTTTGACA
    AACACCTCCA CCGCTGAACC CCACCAACCC CCCCAGGCCT ATCACACAGG CTTGGAGCCA
    GAAGTCCAAA AGACAGTGAT GGACGGCGGA GCTGCAGATC AGTGTCCCGG TCCTGCCCGA
    CCACGGCTCG GCTCGGCTCC CTCCTCCCAG GGTTCAGAGA CCTCCGTTCT TCCCCGTCAG
    GCCAGCCTCT CAAATCCCAG AGGAGAGTTT TCAGATTCCT CCACCTCGTC CTCAGCCTCC
    CCTCCTCCCC CATCGGGTTT CTACCCCAAA GTGTCCCCCC CAGTGTCCCC ATCACGCTCC
    ATCTCTCCTA CGTTGAAAGC GGGGAAAACA GCCTTCACTG TGGGCCGCTT CCAGGTGACC
    CCATCAACAC CCGTTTCTGC TGTCAGTCAT CATCAGCCAC GCCCCTTTAG TGATGCTTCA
    CCCATCGCTC ACTCCCCCCC TCCTACTCAG AAGAACCAAT CAGAGAGCAG CTCAAGAAGC
    AGTTCAGAAG AAGACAGCCA ATCAGAAATC AGCAACCACA CAGTCATCAA TACAACATAT
    GGTTACCGTG ACAACCCCAC CAGTCAAGAC GAGAAGTGCA TGAGCAGTCT TAAACTGGGA
    ATGACTATGT GGGAGGGGCC AGCCAACAGC CAATCACGTA GCCGTTCTGC TACTTACAGC
    TCTGACGAAT CAGAGGGTGA GGAGATGTGG GAGGAGCTAC TGGAGCTTCG TAAAAGACAC
    TTGGTGGAGG TCCAGAATCT ACAGATGAGT CAAAAACAAG AAATGGAGGA GTTGTACAGA
    CGGAAGGGTA AAGCCCTACC ACCTGGGATC GTCCCTCCAG CCGCCATGCT GAACCACCGC
    CAACGCCGCC TCTCCAAGAC AGGAAACTAT CCTCTGGCAC GCCGAAGCAG CCTCCAGAGA
    CTAGATCTGC CGCCCCCTGC AGGGATCATG AGGAAGGGCT CGGTCAGCGG TAGCAGTAGT
    GGATCTCAAG ACCGGATCCT GAAAGGAGTG ACGTTTGCTC CTGGTCACAG CTGCATGGTG
    AGCAACAGAG GACCAGAACC AGCTCCATAC CCGGATCTCT GTAGAAGTCA GGACCAGAAT
    CACAGTCAGG ACCAGAATCA CAGTCAGGAC CAGGACTACG ACCAAATTCA CAGTCAGGAC
    GAGGTTCAGG TCCAGGTCCA GGACCAGGGG CCTCATTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020556306.1
    CDS218..3739
    Translation

    Target ORF information:

    RefSeq Version XM_020700647.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes serine/threonine-protein kinase WNK4-like (LOC101162572), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_020700647.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGACGACA AGGAGAAGCT CCGGGGGAAG TATAAAGACA ACGACGCCAT CGAGTTCCTG 
    TACGAACTCT ACAAGGATGT GCCAGAGGAG GTCGCCCAGG AGATGGTCAT TTTGGGATTT
    GTGTTCAAAG CAGACTACAA GGTGATTGCC AAAGCCATTC GGCATCGAGT GACAGCCATA
    AAGTATCAGC GGGAAAAACG GCGCCTCCTG CTGGAGGAAG GAGTGAACAG TCAGAAGCAA
    ACTGACATCG AGGAAGAGCT TCATCTTCAT CAACGAGCTG AGCTGTCCAA TCAGACGCTC
    CCAGTTTCAA CAGGAGCCAG AGGAGCGGCC AGTCAGGGGG ACTCTGCTGC ATCCACACAA
    GCTCCGCCCC TGACTGCAAC AGCACAAGCT CCGCCCCTGA CTGCATCAGC ACAAGCTCCG
    CCTCTGACTG CAACAGCACA AGCTCCGCCC CTGACTGCAT CAGCACAAGC TCCGCCCCTG
    ACTGCAACAG CACAAGCTCC GCCCCTGACT GCATCAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CACGCTCCTT CTCTAACTGC ATCGGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCCAC ACACGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGATTGCAT CCACACACTC TCCTTCCCTA ACTGCATCGG CACAAGCTCC ACCCCTGACT
    GCATCCACAC ACGCTCCTTC CCTAACTGCA TCGGCACAAG CTCCGCCCCT GACTGCATCG
    GCACAAACTC CGCCCCTGAC TGCATCGGCA CAAGCTCCGC CCCTGACTGC ATCAGCACAA
    GCTCCGCCCC TGACTGCATC CACACACGCT CCGCCCCTGA CTTTATCAGC ACAAGCTCCG
    CCCCTGACTG CATCAGCACA AGCTCCGCCC CTGACTGCAT CCACACAAGC TCCGCCCAGC
    AGCCGGGGGG ATTCCGGGAT CAACACTGTC TCCAGCAGAG CTGATGGCGA CACCGAGGAG
    GACGGTGCGA TCCGACTAGT CTCCCACCCC CCCACTGCCT ACCATGTGAT TGAAACCACG
    CCTCTTCTTG TTCCTAACCC CACTCCTCCA TCAGTACCTC CACAGACGGA GGTTCCTTCT
    GTTTTGACCC GCACAGCTTC CAAAGCTCCG CCCTTTCCCA TACTACGCTT TCCTAAAAGC
    ATTGCTGTGT CCCAGGCTGC AGTAAAGCCC CCCCCTGAGC CCTCCTATGA TTTCTCTTCA
    CCTGTCGACA GCTGCTCTTC TGACTTCACC TCGGGCTTGA GTGATGGCAA TGACGGCCAA
    TCAGAGAGGA GCAGGATGGA GGCAACGATG AGGGAAGCAA CCAAACAGTT TAGAAGGAGA
    GCCAGAGCTC GTCTCCGAAT CATAGGGGTG TCTGACAAGG TGGACCGAGT GGTGGAGTGT
    CAGCTGCAGA CTCACAACAA CAAGATGGTC ACGTTTAAGT TTGACCTGGA TGGAGACAAT
    CCCGAGGACA TCGCATCTGT GATGATTCAC AGAGATTTTA TCTTACCGTC TGAGCGAGAA
    GGATTTATCC TCCGCATGTA TGACATCATC CAGAGGGCGG AGTCTATGAT ACACCAGCAT
    CATCCTGCAG TCTTGTACCA GCCTTCTCAG CTGATGACCT CTGCTCCCCA GTCAAACTGC
    CTCTGCTTTC AACCTTTTCA GCCAAAGTCA GCAGTGACTA ACCTGTCCAG GACTTTGTCT
    TCCTCCTCCT TACCTGACAT AGATGAAAGT GACACCACCC CCTCAAAGGC AATTGACTTC
    AGCATTGACC CTGATGCTGT TCCCCCCGTG AGGCCGTTGA GGTCACAGTC CTTCCAGACG
    TCATCAGCCT CTTCCTATGA GCCCCCCCTT TACCCTTACC ATTACCCCCA TCCTGAATCT
    ACCACTCCCC CCCTCTCTCA GAATCTGCTT CCTTCTCATC TTTACTCCAC CTATACAGCC
    TCTTTCTCCA CGCCCAGCTC CGCCCCCCAG ATTCCACGAG TCCACAGCAA CCCCTCCCTC
    TTTACCTCCA CACCATCATC TTTAGCCCCT CCCTCTCCTA GGTGGCCACC CCCTGACCAG
    CCTCTCTTCT CTTTGGCCAA TGTACTGTCT GTGGCCATGA GTGTGGCCCA CAGCCTCATG
    CCCCCACCCG GACATCCGAG TCAAGGCTTT CAACACCAGG CTCCTCCCCC GCCTTTCCCC
    CAGTCCACGT TCTCACCCCC CATGTCTCCA TCTCTAAAAC GGAACCTTCA GACTTTGACA
    AACACCTCCA CCGCTGAACC CCACCAACCC CCCCAGGCCT ATCACACAGG CTTGGAGCCA
    GAAGTCCAAA AGACAGTGAT GGACGGCGGA GCTGCAGATC AGTGTCCCGG TCCTGCCCGA
    CCACGGCTCG GCTCGGCTCC CTCCTCCCAG GGTTCAGAGA CCTCCGTTCT TCCCCGTCAG
    GCCAGCCTCT CAAATCCCAG AGGAGAGTTT TCAGATTCCT CCACCTCGTC CTCAGCCTCC
    CCTCCTCCCC CATCGGGTTT CTACCCCAAA GTGTCCCCCC CAGTGTCCCC ATCACGCTCC
    ATCTCTCCTA CGTTGAAAGC GGGGAAAACA GCCTTCACTG TGGGCCGCTT CCAGGTGACC
    CCATCAACAC CCGTTTCTGC TGTCAGTCAT CATCAGCCAC GCCCCTTTAG TGATGCTTCA
    CCCATCGCTC ACTCCCCCCC TCCTACTCAG AAGAACCAAT CAGAGAGCAG CTCAAGAAGC
    AGTTCAGAAG AAGACAGCCA ATCAGAAATC AGCAACCACA CAGTCATCAA TACAACATAT
    GGTTACCGTG ACAACCCCAC CAGTCAAGAC GAGAAGTGCA TGAGCAGTCT TAAACTGGGA
    ATGACTATGT GGGAGGGGCC AGCCAACAGC CAATCACGTA GCCGTTCTGC TACTTACAGC
    TCTGACGAAT CAGAGGGTGA GGAGATGTGG GAGGAGCTAC TGGAGCTTCG TAAAAGACAC
    TTGGTGGAGG TCCAGAATCT ACAGATGAGT CAAAAACAAG AAATGGAGGA GTTGTACAGA
    CGGAAGGGTA AAGCCCTACC ACCTGGGATC GTCCCTCCAG CCGCCATGCT GAACCACCGC
    CAACGCCGCC TCTCCAAGAC AGGAAACTAT CCTCTGGCAC GCCGAAGCAG CCTCCAGAGA
    CTAGATCTGC CGCCCCCTGC AGGGATCATG AGGAAGGGCT CGGTCAGCGG TAGCAGTAGT
    GGATCTCAAG ACCGGATCCT GAAAGGAGTG ACGTTTGCTC CTGGTCACAG CTGCATGGTG
    AGCAACAGAG GACCAGAACC AGCTCCATAC CCGGATCTCT GTAGAAGTCA GGACCAGAAT
    CACAGTCAGG ACCAGAATCA CAGTCAGGAC CAGGACTACG ACCAAATTCA CAGTCAGGAC
    GAGGTTCAGG TCCAGGTCCA GGACCAGGGG CCTCATTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa68001
    Clone ID Related Accession (Same CDS sequence) XM_020700648.1
    Accession Version XM_020700648.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1491408000000
    Organism Oryzias latipes(Japanese medaka)
    Product serine/threonine-protein kinase WNK4-like isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004090421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGACGACA AGGAGAAGCT CCGGGGGAAG TATAAAGACA ACGACGCCAT CGAGTTCCTG 
    TACGAACTCT ACAAGGATGT GCCAGAGGAG GTCGCCCAGG AGATGGTCAT TTTGGGATTT
    GTGTTCAAAG CAGACTACAA GGTGATTGCC AAAGCCATTC GGCATCGAGT GACAGCCATA
    AAGTATCAGC GGGAAAAACG GCGCCTCCTG CTGGAGGAAG GAGTGAACAG TCAGAAGCAA
    ACTGACATCG AGGAAGAGCT TCATCTTCAT CAACGAGCTG AGCTGTCCAA TCAGACGCTC
    CCAGTTTCAA CAGGAGCCAG AGGAGCGGCC AGTCAGGGGG ACTCTGCTGC ATCCACACAA
    GCTCCGCCCC TGACTGCAAC AGCACAAGCT CCGCCCCTGA CTGCATCAGC ACAAGCTCCG
    CCTCTGACTG CAACAGCACA AGCTCCGCCC CTGACTGCAT CAGCACAAGC TCCGCCCCTG
    ACTGCAACAG CACAAGCTCC GCCCCTGACT GCATCAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CACGCTCCTT CTCTAACTGC ATCGGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCCAC ACACGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGATTGCAT CCACACACTC TCCTTCCCTA ACTGCATCGG CACAAGCTCC ACCCCTGACT
    GCATCCACAC ACGCTCCTTC CCTAACTGCA TCGGCACAAG CTCCGCCCCT GACTGCATCG
    GCACAAACTC CGCCCCTGAC TGCATCGGCA CAAGCTCCGC CCCTGACTGC ATCAGCACAA
    GCTCCGCCCC TGACTGCATC CACACACGCT CCGCCCCTGA CTTTATCAGC ACAAGCTCCG
    CCCCTGACTG CATCAGCACA AGCTCCGCCC CTGACTGCAT CCACACAAGC TCCGCCCAGC
    AGCCGGGGGG ATTCCGGGAT CAACACTGTC TCCAGCAGAG CTGATGGCGA CACCGAGGAG
    GACGGTGCGA TCCGACTAGT CTCCCACCCC CCCACTGCCT CAGACCATGT GATTGAAACC
    ACGCCTCTTC TTGTTCCTAA CCCCACTCCT CCATCAGTAC CTCCACAGAC GGAGGTTCCT
    TCTGTTTTGA CCCGCACAGC TTCCAAAGCT CCGCCCTTTC CCATACTACG CTTTCCTAAA
    AGCATTGCTG TGTCCCAGGC TGCAGTAAAG CCCCCCCCTG AGCCCTCCTA TGATTTCTCT
    TCACCTGTCG ACAGCTGCTC TTCTGACTTC ACCTCGGGCT TGAGTGATGG CAATGACGGC
    CAATCAGAGA GGAGCAGGAT GGAGGCAACG ATGAGGGAAG CAACCAAACA GTTTAGAAGG
    AGAGCCAGAG CTCGTCTCCG AATCATAGGG GTGTCTGACA AGGTGGACCG AGTGGTGGAG
    TGTCAGCTGC AGACTCACAA CAACAAGATG GTCACGTTTA AGTTTGACCT GGATGGAGAC
    AATCCCGAGG ACATCGCATC TGTGATGATT CACAGAGATT TTATCTTACC GTCTGAGCGA
    GAAGGATTTA TCCTCCGCAT GTATGACATC ATCCAGAGGG CGGAGTCTAT GATACACCAG
    CATCATCCTG CAGTCTTGTA CCAGCCTTCT CAGCTGATGA CCTCTGCTCC CCAGCCAAAG
    TCAGCAGTGA CTAACCTGTC CAGGACTTTG TCTTCCTCCT CCTTACCTGA CATAGATGAA
    AGTGACACCA CCCCCTCAAA GGCAATTGAC TTCAGCATTG ACCCTGATGC TGTTCCCCCC
    GTGAGGCCGT TGAGGTCACA GTCCTTCCAG ACGTCATCAG CCTCTTCCTA TGAGCCCCCC
    CTTTACCCTT ACCATTACCC CCATCCTGAA TCTACCACTC CCCCCCTCTC TCAGAATCTG
    CTTCCTTCTC ATCTTTACTC CACCTATACA GCCTCTTTCT CCACGCCCAG CTCCGCCCCC
    CAGATTCCAC GAGTCCACAG CAACCCCTCC CTCTTTACCT CCACACCATC ATCTTTAGCC
    CCTCCCTCTC CTAGGTGGCC ACCCCCTGAC CAGCCTCTCT TCTCTTTGGC CAATGTACTG
    TCTGTGGCCA TGAGTGTGGC CCACAGCCTC ATGCCCCCAC CCGGACATCC GAGTCAAGGC
    TTTCAACACC AGGCTCCTCC CCCGCCTTTC CCCCAGTCCA CGTTCTCACC CCCCATGTCT
    CCATCTCTAA AACGGAACCT TCAGACTTTG ACAAACACCT CCACCGCTGA ACCCCACCAA
    CCCCCCCAGG CCTATCACAC AGGCTTGGAG CCAGAAGTCC AAAAGACAGT GATGGACGGC
    GGAGCTGCAG ATCAGTGTCC CGGTCCTGCC CGACCACGGC TCGGCTCGGC TCCCTCCTCC
    CAGGGTTCAG AGACCTCCGT TCTTCCCCGT CAGGCCAGCC TCTCAAATCC CAGAGGAGAG
    TTTTCAGATT CCTCCACCTC GTCCTCAGCC TCCCCTCCTC CCCCATCGGG TTTCTACCCC
    AAAGTGTCCC CCCCAGTGTC CCCATCACGC TCCATCTCTC CTACGTTGAA AGCGGGGAAA
    ACAGCCTTCA CTGTGGGCCG CTTCCAGGTG ACCCCATCAA CACCCGTTTC TGCTGTCAGT
    CATCATCAGC CACGCCCCTT TAGTGATGCT TCACCCATCG CTCACTCCCC CCCTCCTACT
    CAGAAGAACC AATCAGAGAG CAGCTCAAGA AGCAGTTCAG AAGAAGACAG CCAATCAGAA
    ATCAGCAACC ACACAGTCAT CAATACAACA TATGGTTACC GTGACAACCC CACCAGTCAA
    GACGAGAAGT GCATGAGCAG TCTTAAACTG GGAATGACTA TGTGGGAGGG GCCAGCCAAC
    AGCCAATCAC GTAGCCGTTC TGCTACTTAC AGCTCTGACG AATCAGAGGG TGAGGAGATG
    TGGGAGGAGC TACTGGAGCT TCGTAAAAGA CACTTGGTGG AGGTCCAGAA TCTACAGATG
    AGTCAAAAAC AAGAAATGGA GGAGTTGTAC AGACGGAAGG GTAAAGCCCT ACCACCTGGG
    ATCGTCCCTC CAGCCGCCAT GCTGAACCAC CGCCAACGCC GCCTCTCCAA GACAGGAAAC
    TATCCTCTGG CACGCCGAAG CAGCCTCCAG AGACTAGATC TGCCGCCCCC TGCAGGGATC
    ATGAGGAAGG GCTCGGTCAG CGGTAGCAGT AGTGGATCTC AAGACCGGAT CCTGAAAGGA
    GTGACGTTTG CTCCTGGTCA CAGCTGCATG GTGAGCAACA GAGGACCAGA ACCAGCTCCA
    TACCCGGATC TCTGTAGAAG TCAGGACCAG AATCACAGTC AGGACCAGAA TCACAGTCAG
    GACCAGGACT ACGACCAAAT TCACAGTCAG GACGAGGTTC AGGTCCAGGT CCAGGACCAG
    GGGCCTCATT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020556307.1
    CDS211..3705
    Translation

    Target ORF information:

    RefSeq Version XM_020700648.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes serine/threonine-protein kinase WNK4-like (LOC101162572), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_020700648.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGACGACA AGGAGAAGCT CCGGGGGAAG TATAAAGACA ACGACGCCAT CGAGTTCCTG 
    TACGAACTCT ACAAGGATGT GCCAGAGGAG GTCGCCCAGG AGATGGTCAT TTTGGGATTT
    GTGTTCAAAG CAGACTACAA GGTGATTGCC AAAGCCATTC GGCATCGAGT GACAGCCATA
    AAGTATCAGC GGGAAAAACG GCGCCTCCTG CTGGAGGAAG GAGTGAACAG TCAGAAGCAA
    ACTGACATCG AGGAAGAGCT TCATCTTCAT CAACGAGCTG AGCTGTCCAA TCAGACGCTC
    CCAGTTTCAA CAGGAGCCAG AGGAGCGGCC AGTCAGGGGG ACTCTGCTGC ATCCACACAA
    GCTCCGCCCC TGACTGCAAC AGCACAAGCT CCGCCCCTGA CTGCATCAGC ACAAGCTCCG
    CCTCTGACTG CAACAGCACA AGCTCCGCCC CTGACTGCAT CAGCACAAGC TCCGCCCCTG
    ACTGCAACAG CACAAGCTCC GCCCCTGACT GCATCAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CACGCTCCTT CTCTAACTGC ATCGGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCCAC ACACGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGATTGCAT CCACACACTC TCCTTCCCTA ACTGCATCGG CACAAGCTCC ACCCCTGACT
    GCATCCACAC ACGCTCCTTC CCTAACTGCA TCGGCACAAG CTCCGCCCCT GACTGCATCG
    GCACAAACTC CGCCCCTGAC TGCATCGGCA CAAGCTCCGC CCCTGACTGC ATCAGCACAA
    GCTCCGCCCC TGACTGCATC CACACACGCT CCGCCCCTGA CTTTATCAGC ACAAGCTCCG
    CCCCTGACTG CATCAGCACA AGCTCCGCCC CTGACTGCAT CCACACAAGC TCCGCCCAGC
    AGCCGGGGGG ATTCCGGGAT CAACACTGTC TCCAGCAGAG CTGATGGCGA CACCGAGGAG
    GACGGTGCGA TCCGACTAGT CTCCCACCCC CCCACTGCCT CAGACCATGT GATTGAAACC
    ACGCCTCTTC TTGTTCCTAA CCCCACTCCT CCATCAGTAC CTCCACAGAC GGAGGTTCCT
    TCTGTTTTGA CCCGCACAGC TTCCAAAGCT CCGCCCTTTC CCATACTACG CTTTCCTAAA
    AGCATTGCTG TGTCCCAGGC TGCAGTAAAG CCCCCCCCTG AGCCCTCCTA TGATTTCTCT
    TCACCTGTCG ACAGCTGCTC TTCTGACTTC ACCTCGGGCT TGAGTGATGG CAATGACGGC
    CAATCAGAGA GGAGCAGGAT GGAGGCAACG ATGAGGGAAG CAACCAAACA GTTTAGAAGG
    AGAGCCAGAG CTCGTCTCCG AATCATAGGG GTGTCTGACA AGGTGGACCG AGTGGTGGAG
    TGTCAGCTGC AGACTCACAA CAACAAGATG GTCACGTTTA AGTTTGACCT GGATGGAGAC
    AATCCCGAGG ACATCGCATC TGTGATGATT CACAGAGATT TTATCTTACC GTCTGAGCGA
    GAAGGATTTA TCCTCCGCAT GTATGACATC ATCCAGAGGG CGGAGTCTAT GATACACCAG
    CATCATCCTG CAGTCTTGTA CCAGCCTTCT CAGCTGATGA CCTCTGCTCC CCAGCCAAAG
    TCAGCAGTGA CTAACCTGTC CAGGACTTTG TCTTCCTCCT CCTTACCTGA CATAGATGAA
    AGTGACACCA CCCCCTCAAA GGCAATTGAC TTCAGCATTG ACCCTGATGC TGTTCCCCCC
    GTGAGGCCGT TGAGGTCACA GTCCTTCCAG ACGTCATCAG CCTCTTCCTA TGAGCCCCCC
    CTTTACCCTT ACCATTACCC CCATCCTGAA TCTACCACTC CCCCCCTCTC TCAGAATCTG
    CTTCCTTCTC ATCTTTACTC CACCTATACA GCCTCTTTCT CCACGCCCAG CTCCGCCCCC
    CAGATTCCAC GAGTCCACAG CAACCCCTCC CTCTTTACCT CCACACCATC ATCTTTAGCC
    CCTCCCTCTC CTAGGTGGCC ACCCCCTGAC CAGCCTCTCT TCTCTTTGGC CAATGTACTG
    TCTGTGGCCA TGAGTGTGGC CCACAGCCTC ATGCCCCCAC CCGGACATCC GAGTCAAGGC
    TTTCAACACC AGGCTCCTCC CCCGCCTTTC CCCCAGTCCA CGTTCTCACC CCCCATGTCT
    CCATCTCTAA AACGGAACCT TCAGACTTTG ACAAACACCT CCACCGCTGA ACCCCACCAA
    CCCCCCCAGG CCTATCACAC AGGCTTGGAG CCAGAAGTCC AAAAGACAGT GATGGACGGC
    GGAGCTGCAG ATCAGTGTCC CGGTCCTGCC CGACCACGGC TCGGCTCGGC TCCCTCCTCC
    CAGGGTTCAG AGACCTCCGT TCTTCCCCGT CAGGCCAGCC TCTCAAATCC CAGAGGAGAG
    TTTTCAGATT CCTCCACCTC GTCCTCAGCC TCCCCTCCTC CCCCATCGGG TTTCTACCCC
    AAAGTGTCCC CCCCAGTGTC CCCATCACGC TCCATCTCTC CTACGTTGAA AGCGGGGAAA
    ACAGCCTTCA CTGTGGGCCG CTTCCAGGTG ACCCCATCAA CACCCGTTTC TGCTGTCAGT
    CATCATCAGC CACGCCCCTT TAGTGATGCT TCACCCATCG CTCACTCCCC CCCTCCTACT
    CAGAAGAACC AATCAGAGAG CAGCTCAAGA AGCAGTTCAG AAGAAGACAG CCAATCAGAA
    ATCAGCAACC ACACAGTCAT CAATACAACA TATGGTTACC GTGACAACCC CACCAGTCAA
    GACGAGAAGT GCATGAGCAG TCTTAAACTG GGAATGACTA TGTGGGAGGG GCCAGCCAAC
    AGCCAATCAC GTAGCCGTTC TGCTACTTAC AGCTCTGACG AATCAGAGGG TGAGGAGATG
    TGGGAGGAGC TACTGGAGCT TCGTAAAAGA CACTTGGTGG AGGTCCAGAA TCTACAGATG
    AGTCAAAAAC AAGAAATGGA GGAGTTGTAC AGACGGAAGG GTAAAGCCCT ACCACCTGGG
    ATCGTCCCTC CAGCCGCCAT GCTGAACCAC CGCCAACGCC GCCTCTCCAA GACAGGAAAC
    TATCCTCTGG CACGCCGAAG CAGCCTCCAG AGACTAGATC TGCCGCCCCC TGCAGGGATC
    ATGAGGAAGG GCTCGGTCAG CGGTAGCAGT AGTGGATCTC AAGACCGGAT CCTGAAAGGA
    GTGACGTTTG CTCCTGGTCA CAGCTGCATG GTGAGCAACA GAGGACCAGA ACCAGCTCCA
    TACCCGGATC TCTGTAGAAG TCAGGACCAG AATCACAGTC AGGACCAGAA TCACAGTCAG
    GACCAGGACT ACGACCAAAT TCACAGTCAG GACGAGGTTC AGGTCCAGGT CCAGGACCAG
    GGGCCTCATT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa68002
    Clone ID Related Accession (Same CDS sequence) XM_020700649.1
    Accession Version XM_020700649.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3489bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1491408000000
    Organism Oryzias latipes(Japanese medaka)
    Product serine/threonine-protein kinase WNK4-like isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004090421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGACGACA AGGAGAAGCT CCGGGGGAAG TATAAAGACA ACGACGCCAT CGAGTTCCTG 
    TACGAACTCT ACAAGGATGT GCCAGAGGAG GTCGCCCAGG AGATGGTCAT TTTGGGATTT
    GTGTTCAAAG CAGACTACAA GGTGATTGCC AAAGCCATTC GGCATCGAGT GACAGCCATA
    AAGTATCAGC GGGAAAAACG GCGCCTCCTG CTGGAGGAAG GAGTGAACAG TCAGAAGCAA
    ACTGACATCG AGGAAGAGCT TCATCTTCAT CAACGAGCTG AGCTGTCCAA TCAGACGCTC
    CCAGTTTCAA CAGGAGCCAG AGGAGCGGCC AGTCAGGGGG ACTCTGCTGC ATCCACACAA
    GCTCCGCCCC TGACTGCAAC AGCACAAGCT CCGCCCCTGA CTGCATCAGC ACAAGCTCCG
    CCTCTGACTG CAACAGCACA AGCTCCGCCC CTGACTGCAT CAGCACAAGC TCCGCCCCTG
    ACTGCAACAG CACAAGCTCC GCCCCTGACT GCATCAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CACGCTCCTT CTCTAACTGC ATCGGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCCAC ACACGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGATTGCAT CCACACACTC TCCTTCCCTA ACTGCATCGG CACAAGCTCC ACCCCTGACT
    GCATCCACAC ACGCTCCTTC CCTAACTGCA TCGGCACAAG CTCCGCCCCT GACTGCATCG
    GCACAAACTC CGCCCCTGAC TGCATCGGCA CAAGCTCCGC CCCTGACTGC ATCAGCACAA
    GCTCCGCCCC TGACTGCATC CACACACGCT CCGCCCCTGA CTTTATCAGC ACAAGCTCCG
    CCCCTGACTG CATCAGCACA AGCTCCGCCC CTGACTGCAT CCACACAAGC TCCGCCCAGC
    AGCCGGGGGG ATTCCGGGAT CAACACTGTC TCCAGCAGAG CTGATGGCGA CACCGAGGAG
    GACGCAGACC ATGTGATTGA AACCACGCCT CTTCTTGTTC CTAACCCCAC TCCTCCATCA
    GTACCTCCAC AGACGGAGGT TCCTTCTGTT TTGACCCGCA CAGCTTCCAA AGCTCCGCCC
    TTTCCCATAC TACGCTTTCC TAAAAGCATT GCTGTGTCCC AGGCTGCAGT AAAGCCCCCC
    CCTGAGCCCT CCTATGATTT CTCTTCACCT GTCGACAGCT GCTCTTCTGA CTTCACCTCG
    GGCTTGAGTG ATGGCAATGA CGGCCAATCA GAGAGGAGCA GGATGGAGGC AACGATGAGG
    GAAGCAACCA AACAGTTTAG AAGGAGAGCC AGAGCTCGTC TCCGAATCAT AGGGGTGTCT
    GACAAGGTGG ACCGAGTGGT GGAGTGTCAG CTGCAGACTC ACAACAACAA GATGGTCACG
    TTTAAGTTTG ACCTGGATGG AGACAATCCC GAGGACATCG CATCTGTGAT GATTCACAGA
    GATTTTATCT TACCGTCTGA GCGAGAAGGA TTTATCCTCC GCATGTATGA CATCATCCAG
    AGGGCGGAGT CTATGATACA CCAGCATCAT CCTGCAGTCT TGTACCAGCC TTCTCAGCTG
    ATGACCTCTG CTCCCCAGTC AAACTGCCTC TGCTTTCAAC CTTTTCAGCC AAAGTCAGCA
    GTGACTAACC TGTCCAGGAC TTTGTCTTCC TCCTCCTTAC CTGACATAGA TGAAAGTGAC
    ACCACCCCCT CAAAGGCAAT TGACTTCAGC ATTGACCCTG ATGCTGTTCC CCCCGTGAGG
    CCGTTGAGGT CACAGTCCTT CCAGACGTCA TCAGCCTCTT CCTATGAGCC CCCCCTTTAC
    CCTTACCATT ACCCCCATCC TGAATCTACC ACTCCCCCCC TCTCTCAGAA TCTGCTTCCT
    TCTCATCTTT ACTCCACCTA TACAGCCTCT TTCTCCACGC CCAGCTCCGC CCCCCAGATT
    CCACGAGTCC ACAGCAACCC CTCCCTCTTT ACCTCCACAC CATCATCTTT AGCCCCTCCC
    TCTCCTAGGT GGCCACCCCC TGACCAGCCT CTCTTCTCTT TGGCCAATGT ACTGTCTGTG
    GCCATGAGTG TGGCCCACAG CCTCATGCCC CCACCCGGAC ATCCGAGTCA AGGCTTTCAA
    CACCAGGCTC CTCCCCCGCC TTTCCCCCAG TCCACGTTCT CACCCCCCAT GTCTCCATCT
    CTAAAACGGA ACCTTCAGAC TTTGACAAAC ACCTCCACCG CTGAACCCCA CCAACCCCCC
    CAGGCCTATC ACACAGGCTT GGAGCCAGAA GTCCAAAAGA CAGTGATGGA CGGCGGAGCT
    GCAGATCAGT GTCCCGGTCC TGCCCGACCA CGGCTCGGCT CGGCTCCCTC CTCCCAGGGT
    TCAGAGACCT CCGTTCTTCC CCGTCAGGCC AGCCTCTCAA ATCCCAGAGG AGAGTTTTCA
    GATTCCTCCA CCTCGTCCTC AGCCTCCCCT CCTCCCCCAT CGGGTTTCTA CCCCAAAGTG
    TCCCCCCCAG TGTCCCCATC ACGCTCCATC TCTCCTACGT TGAAAGCGGG GAAAACAGCC
    TTCACTGTGG GCCGCTTCCA GGTGACCCCA TCAACACCCG TTTCTGCTGT CAGTCATCAT
    CAGCCACGCC CCTTTAGTGA TGCTTCACCC ATCGCTCACT CCCCCCCTCC TACTCAGAAG
    AACCAATCAG AGAGCAGCTC AAGAAGCAGT TCAGAAGAAG ACAGCCAATC AGAAATCAGC
    AACCACACAG TCATCAATAC AACATATGGT TACCGTGACA ACCCCACCAG TCAAGACGAG
    AAGTGCATGA GCAGTCTTAA ACTGGGAATG ACTATGTGGG AGGGGCCAGC CAACAGCCAA
    TCACGTAGCC GTTCTGCTAC TTACAGCTCT GACGAATCAG AGGGTGAGGA GATGTGGGAG
    GAGCTACTGG AGCTTCGTAA AAGACACTTG GTGGAGGTCC AGAATCTACA GATGAGTCAA
    AAACAAGAAA TGGAGGAGTT GTACAGACGG AAGGGTAAAG CCCTACCACC TGGGATCGTC
    CCTCCAGCCG CCATGCTGAA CCACCGCCAA CGCCGCCTCT CCAAGACAGG AAACTATCCT
    CTGGCACGCC GAAGCAGCCT CCAGAGACTA GATCTGCCGC CCCCTGCAGG GATCATGAGG
    AAGGGCTCGG TCAGCGGTAG CAGTAGTGGA TCTCAAGACC GGATCCTGAA AGGAGTGACG
    TTTGCTCCTG GTCACAGCTG CATGGTGAGC AACAGAGGAC CAGAACCAGC TCCATACCCG
    GATCTCTGTA GAAGTCAGGA CCAGAATCAC AGTCAGGACC AGAATCACAG TCAGGACCAG
    GACTACGACC AAATTCACAG TCAGGACGAG GTTCAGGTCC AGGTCCAGGA CCAGGGGCCT
    CATTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020556308.1
    CDS214..3702
    Translation

    Target ORF information:

    RefSeq Version XM_020700649.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes serine/threonine-protein kinase WNK4-like (LOC101162572), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_020700649.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGACGACA AGGAGAAGCT CCGGGGGAAG TATAAAGACA ACGACGCCAT CGAGTTCCTG 
    TACGAACTCT ACAAGGATGT GCCAGAGGAG GTCGCCCAGG AGATGGTCAT TTTGGGATTT
    GTGTTCAAAG CAGACTACAA GGTGATTGCC AAAGCCATTC GGCATCGAGT GACAGCCATA
    AAGTATCAGC GGGAAAAACG GCGCCTCCTG CTGGAGGAAG GAGTGAACAG TCAGAAGCAA
    ACTGACATCG AGGAAGAGCT TCATCTTCAT CAACGAGCTG AGCTGTCCAA TCAGACGCTC
    CCAGTTTCAA CAGGAGCCAG AGGAGCGGCC AGTCAGGGGG ACTCTGCTGC ATCCACACAA
    GCTCCGCCCC TGACTGCAAC AGCACAAGCT CCGCCCCTGA CTGCATCAGC ACAAGCTCCG
    CCTCTGACTG CAACAGCACA AGCTCCGCCC CTGACTGCAT CAGCACAAGC TCCGCCCCTG
    ACTGCAACAG CACAAGCTCC GCCCCTGACT GCATCAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CACGCTCCTT CTCTAACTGC ATCGGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCCAC ACACGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGATTGCAT CCACACACTC TCCTTCCCTA ACTGCATCGG CACAAGCTCC ACCCCTGACT
    GCATCCACAC ACGCTCCTTC CCTAACTGCA TCGGCACAAG CTCCGCCCCT GACTGCATCG
    GCACAAACTC CGCCCCTGAC TGCATCGGCA CAAGCTCCGC CCCTGACTGC ATCAGCACAA
    GCTCCGCCCC TGACTGCATC CACACACGCT CCGCCCCTGA CTTTATCAGC ACAAGCTCCG
    CCCCTGACTG CATCAGCACA AGCTCCGCCC CTGACTGCAT CCACACAAGC TCCGCCCAGC
    AGCCGGGGGG ATTCCGGGAT CAACACTGTC TCCAGCAGAG CTGATGGCGA CACCGAGGAG
    GACGCAGACC ATGTGATTGA AACCACGCCT CTTCTTGTTC CTAACCCCAC TCCTCCATCA
    GTACCTCCAC AGACGGAGGT TCCTTCTGTT TTGACCCGCA CAGCTTCCAA AGCTCCGCCC
    TTTCCCATAC TACGCTTTCC TAAAAGCATT GCTGTGTCCC AGGCTGCAGT AAAGCCCCCC
    CCTGAGCCCT CCTATGATTT CTCTTCACCT GTCGACAGCT GCTCTTCTGA CTTCACCTCG
    GGCTTGAGTG ATGGCAATGA CGGCCAATCA GAGAGGAGCA GGATGGAGGC AACGATGAGG
    GAAGCAACCA AACAGTTTAG AAGGAGAGCC AGAGCTCGTC TCCGAATCAT AGGGGTGTCT
    GACAAGGTGG ACCGAGTGGT GGAGTGTCAG CTGCAGACTC ACAACAACAA GATGGTCACG
    TTTAAGTTTG ACCTGGATGG AGACAATCCC GAGGACATCG CATCTGTGAT GATTCACAGA
    GATTTTATCT TACCGTCTGA GCGAGAAGGA TTTATCCTCC GCATGTATGA CATCATCCAG
    AGGGCGGAGT CTATGATACA CCAGCATCAT CCTGCAGTCT TGTACCAGCC TTCTCAGCTG
    ATGACCTCTG CTCCCCAGTC AAACTGCCTC TGCTTTCAAC CTTTTCAGCC AAAGTCAGCA
    GTGACTAACC TGTCCAGGAC TTTGTCTTCC TCCTCCTTAC CTGACATAGA TGAAAGTGAC
    ACCACCCCCT CAAAGGCAAT TGACTTCAGC ATTGACCCTG ATGCTGTTCC CCCCGTGAGG
    CCGTTGAGGT CACAGTCCTT CCAGACGTCA TCAGCCTCTT CCTATGAGCC CCCCCTTTAC
    CCTTACCATT ACCCCCATCC TGAATCTACC ACTCCCCCCC TCTCTCAGAA TCTGCTTCCT
    TCTCATCTTT ACTCCACCTA TACAGCCTCT TTCTCCACGC CCAGCTCCGC CCCCCAGATT
    CCACGAGTCC ACAGCAACCC CTCCCTCTTT ACCTCCACAC CATCATCTTT AGCCCCTCCC
    TCTCCTAGGT GGCCACCCCC TGACCAGCCT CTCTTCTCTT TGGCCAATGT ACTGTCTGTG
    GCCATGAGTG TGGCCCACAG CCTCATGCCC CCACCCGGAC ATCCGAGTCA AGGCTTTCAA
    CACCAGGCTC CTCCCCCGCC TTTCCCCCAG TCCACGTTCT CACCCCCCAT GTCTCCATCT
    CTAAAACGGA ACCTTCAGAC TTTGACAAAC ACCTCCACCG CTGAACCCCA CCAACCCCCC
    CAGGCCTATC ACACAGGCTT GGAGCCAGAA GTCCAAAAGA CAGTGATGGA CGGCGGAGCT
    GCAGATCAGT GTCCCGGTCC TGCCCGACCA CGGCTCGGCT CGGCTCCCTC CTCCCAGGGT
    TCAGAGACCT CCGTTCTTCC CCGTCAGGCC AGCCTCTCAA ATCCCAGAGG AGAGTTTTCA
    GATTCCTCCA CCTCGTCCTC AGCCTCCCCT CCTCCCCCAT CGGGTTTCTA CCCCAAAGTG
    TCCCCCCCAG TGTCCCCATC ACGCTCCATC TCTCCTACGT TGAAAGCGGG GAAAACAGCC
    TTCACTGTGG GCCGCTTCCA GGTGACCCCA TCAACACCCG TTTCTGCTGT CAGTCATCAT
    CAGCCACGCC CCTTTAGTGA TGCTTCACCC ATCGCTCACT CCCCCCCTCC TACTCAGAAG
    AACCAATCAG AGAGCAGCTC AAGAAGCAGT TCAGAAGAAG ACAGCCAATC AGAAATCAGC
    AACCACACAG TCATCAATAC AACATATGGT TACCGTGACA ACCCCACCAG TCAAGACGAG
    AAGTGCATGA GCAGTCTTAA ACTGGGAATG ACTATGTGGG AGGGGCCAGC CAACAGCCAA
    TCACGTAGCC GTTCTGCTAC TTACAGCTCT GACGAATCAG AGGGTGAGGA GATGTGGGAG
    GAGCTACTGG AGCTTCGTAA AAGACACTTG GTGGAGGTCC AGAATCTACA GATGAGTCAA
    AAACAAGAAA TGGAGGAGTT GTACAGACGG AAGGGTAAAG CCCTACCACC TGGGATCGTC
    CCTCCAGCCG CCATGCTGAA CCACCGCCAA CGCCGCCTCT CCAAGACAGG AAACTATCCT
    CTGGCACGCC GAAGCAGCCT CCAGAGACTA GATCTGCCGC CCCCTGCAGG GATCATGAGG
    AAGGGCTCGG TCAGCGGTAG CAGTAGTGGA TCTCAAGACC GGATCCTGAA AGGAGTGACG
    TTTGCTCCTG GTCACAGCTG CATGGTGAGC AACAGAGGAC CAGAACCAGC TCCATACCCG
    GATCTCTGTA GAAGTCAGGA CCAGAATCAC AGTCAGGACC AGAATCACAG TCAGGACCAG
    GACTACGACC AAATTCACAG TCAGGACGAG GTTCAGGTCC AGGTCCAGGA CCAGGGGCCT
    CATTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa68003
    Clone ID Related Accession (Same CDS sequence) XM_020700650.1
    Accession Version XM_020700650.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1491408000000
    Organism Oryzias latipes(Japanese medaka)
    Product serine/threonine-protein kinase WNK4-like isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004090421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGACGACA AGGAGAAGCT CCGGGGGAAG TATAAAGACA ACGACGCCAT CGAGTTCCTG 
    TACGAACTCT ACAAGGATGT GCCAGAGGAG GTCGCCCAGG AGATGGTCAT TTTGGGATTT
    GTGTTCAAAG CAGACTACAA GGTGATTGCC AAAGCCATTC GGCATCGAGT GACAGCCATA
    AAGTATCAGC GGGAAAAACG GCGCCTCCTG CTGGAGGAAG GAGTGAACAG TCAGAAGCAA
    ACTGACATCG AGGAAGAGCT TCATCTTCAT CAACGAGCTG AGCTGTCCAA TCAGACGCTC
    CCAGTTTCAA CAGGAGCCAG AGGAGCGGCC AGTCAGGGGG ACTCTGCTGC ATCCACACAA
    GCTCCGCCCC TGACTGCAAC AGCACAAGCT CCGCCCCTGA CTGCATCAGC ACAAGCTCCG
    CCTCTGACTG CAACAGCACA AGCTCCGCCC CTGACTGCAT CAGCACAAGC TCCGCCCCTG
    ACTGCAACAG CACAAGCTCC GCCCCTGACT GCATCAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CACGCTCCTT CTCTAACTGC ATCGGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCCAC ACACGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGATTGCAT CCACACACTC TCCTTCCCTA ACTGCATCGG CACAAGCTCC ACCCCTGACT
    GCATCCACAC ACGCTCCTTC CCTAACTGCA TCGGCACAAG CTCCGCCCCT GACTGCATCG
    GCACAAACTC CGCCCCTGAC TGCATCGGCA CAAGCTCCGC CCCTGACTGC ATCAGCACAA
    GCTCCGCCCC TGACTGCATC CACACACGCT CCGCCCCTGA CTTTATCAGC ACAAGCTCCG
    CCCCTGACTG CATCAGCACA AGCTCCGCCC CTGACTGCAT CCACACAAGC TCCGCCCAGC
    AGCCGGGGGG ATTCCGGGAT CAACACTGTC TCCAGCAGAG CTGATGGCGA CACCGAGGAG
    GACGACCATG TGATTGAAAC CACGCCTCTT CTTGTTCCTA ACCCCACTCC TCCATCAGTA
    CCTCCACAGA CGGAGGTTCC TTCTGTTTTG ACCCGCACAG CTTCCAAAGC TCCGCCCTTT
    CCCATACTAC GCTTTCCTAA AAGCATTGCT GTGTCCCAGG CTGCAGTAAA GCCCCCCCCT
    GAGCCCTCCT ATGATTTCTC TTCACCTGTC GACAGCTGCT CTTCTGACTT CACCTCGGGC
    TTGAGTGATG GCAATGACGG CCAATCAGAG AGGAGCAGGA TGGAGGCAAC GATGAGGGAA
    GCAACCAAAC AGTTTAGAAG GAGAGCCAGA GCTCGTCTCC GAATCATAGG GGTGTCTGAC
    AAGGTGGACC GAGTGGTGGA GTGTCAGCTG CAGACTCACA ACAACAAGAT GGTCACGTTT
    AAGTTTGACC TGGATGGAGA CAATCCCGAG GACATCGCAT CTGTGATGAT TCACAGAGAT
    TTTATCTTAC CGTCTGAGCG AGAAGGATTT ATCCTCCGCA TGTATGACAT CATCCAGAGG
    GCGGAGTCTA TGATACACCA GCATCATCCT GCAGTCTTGT ACCAGCCTTC TCAGCTGATG
    ACCTCTGCTC CCCAGTCAAA CTGCCTCTGC TTTCAACCTT TTCAGCCAAA GTCAGCAGTG
    ACTAACCTGT CCAGGACTTT GTCTTCCTCC TCCTTACCTG ACATAGATGA AAGTGACACC
    ACCCCCTCAA AGGCAATTGA CTTCAGCATT GACCCTGATG CTGTTCCCCC CGTGAGGCCG
    TTGAGGTCAC AGTCCTTCCA GACGTCATCA GCCTCTTCCT ATGAGCCCCC CCTTTACCCT
    TACCATTACC CCCATCCTGA ATCTACCACT CCCCCCCTCT CTCAGAATCT GCTTCCTTCT
    CATCTTTACT CCACCTATAC AGCCTCTTTC TCCACGCCCA GCTCCGCCCC CCAGATTCCA
    CGAGTCCACA GCAACCCCTC CCTCTTTACC TCCACACCAT CATCTTTAGC CCCTCCCTCT
    CCTAGGTGGC CACCCCCTGA CCAGCCTCTC TTCTCTTTGG CCAATGTACT GTCTGTGGCC
    ATGAGTGTGG CCCACAGCCT CATGCCCCCA CCCGGACATC CGAGTCAAGG CTTTCAACAC
    CAGGCTCCTC CCCCGCCTTT CCCCCAGTCC ACGTTCTCAC CCCCCATGTC TCCATCTCTA
    AAACGGAACC TTCAGACTTT GACAAACACC TCCACCGCTG AACCCCACCA ACCCCCCCAG
    GCCTATCACA CAGGCTTGGA GCCAGAAGTC CAAAAGACAG TGATGGACGG CGGAGCTGCA
    GATCAGTGTC CCGGTCCTGC CCGACCACGG CTCGGCTCGG CTCCCTCCTC CCAGGGTTCA
    GAGACCTCCG TTCTTCCCCG TCAGGCCAGC CTCTCAAATC CCAGAGGAGA GTTTTCAGAT
    TCCTCCACCT CGTCCTCAGC CTCCCCTCCT CCCCCATCGG GTTTCTACCC CAAAGTGTCC
    CCCCCAGTGT CCCCATCACG CTCCATCTCT CCTACGTTGA AAGCGGGGAA AACAGCCTTC
    ACTGTGGGCC GCTTCCAGGT GACCCCATCA ACACCCGTTT CTGCTGTCAG TCATCATCAG
    CCACGCCCCT TTAGTGATGC TTCACCCATC GCTCACTCCC CCCCTCCTAC TCAGAAGAAC
    CAATCAGAGA GCAGCTCAAG AAGCAGTTCA GAAGAAGACA GCCAATCAGA AATCAGCAAC
    CACACAGTCA TCAATACAAC ATATGGTTAC CGTGACAACC CCACCAGTCA AGACGAGAAG
    TGCATGAGCA GTCTTAAACT GGGAATGACT ATGTGGGAGG GGCCAGCCAA CAGCCAATCA
    CGTAGCCGTT CTGCTACTTA CAGCTCTGAC GAATCAGAGG GTGAGGAGAT GTGGGAGGAG
    CTACTGGAGC TTCGTAAAAG ACACTTGGTG GAGGTCCAGA ATCTACAGAT GAGTCAAAAA
    CAAGAAATGG AGGAGTTGTA CAGACGGAAG GGTAAAGCCC TACCACCTGG GATCGTCCCT
    CCAGCCGCCA TGCTGAACCA CCGCCAACGC CGCCTCTCCA AGACAGGAAA CTATCCTCTG
    GCACGCCGAA GCAGCCTCCA GAGACTAGAT CTGCCGCCCC CTGCAGGGAT CATGAGGAAG
    GGCTCGGTCA GCGGTAGCAG TAGTGGATCT CAAGACCGGA TCCTGAAAGG AGTGACGTTT
    GCTCCTGGTC ACAGCTGCAT GGTGAGCAAC AGAGGACCAG AACCAGCTCC ATACCCGGAT
    CTCTGTAGAA GTCAGGACCA GAATCACAGT CAGGACCAGA ATCACAGTCA GGACCAGGAC
    TACGACCAAA TTCACAGTCA GGACGAGGTT CAGGTCCAGG TCCAGGACCA GGGGCCTCAT
    TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020556309.1
    CDS217..3702
    Translation

    Target ORF information:

    RefSeq Version XM_020700650.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes serine/threonine-protein kinase WNK4-like (LOC101162572), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_020700650.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGACGACA AGGAGAAGCT CCGGGGGAAG TATAAAGACA ACGACGCCAT CGAGTTCCTG 
    TACGAACTCT ACAAGGATGT GCCAGAGGAG GTCGCCCAGG AGATGGTCAT TTTGGGATTT
    GTGTTCAAAG CAGACTACAA GGTGATTGCC AAAGCCATTC GGCATCGAGT GACAGCCATA
    AAGTATCAGC GGGAAAAACG GCGCCTCCTG CTGGAGGAAG GAGTGAACAG TCAGAAGCAA
    ACTGACATCG AGGAAGAGCT TCATCTTCAT CAACGAGCTG AGCTGTCCAA TCAGACGCTC
    CCAGTTTCAA CAGGAGCCAG AGGAGCGGCC AGTCAGGGGG ACTCTGCTGC ATCCACACAA
    GCTCCGCCCC TGACTGCAAC AGCACAAGCT CCGCCCCTGA CTGCATCAGC ACAAGCTCCG
    CCTCTGACTG CAACAGCACA AGCTCCGCCC CTGACTGCAT CAGCACAAGC TCCGCCCCTG
    ACTGCAACAG CACAAGCTCC GCCCCTGACT GCATCAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CACGCTCCTT CTCTAACTGC ATCGGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCCAC ACACGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGATTGCAT CCACACACTC TCCTTCCCTA ACTGCATCGG CACAAGCTCC ACCCCTGACT
    GCATCCACAC ACGCTCCTTC CCTAACTGCA TCGGCACAAG CTCCGCCCCT GACTGCATCG
    GCACAAACTC CGCCCCTGAC TGCATCGGCA CAAGCTCCGC CCCTGACTGC ATCAGCACAA
    GCTCCGCCCC TGACTGCATC CACACACGCT CCGCCCCTGA CTTTATCAGC ACAAGCTCCG
    CCCCTGACTG CATCAGCACA AGCTCCGCCC CTGACTGCAT CCACACAAGC TCCGCCCAGC
    AGCCGGGGGG ATTCCGGGAT CAACACTGTC TCCAGCAGAG CTGATGGCGA CACCGAGGAG
    GACGACCATG TGATTGAAAC CACGCCTCTT CTTGTTCCTA ACCCCACTCC TCCATCAGTA
    CCTCCACAGA CGGAGGTTCC TTCTGTTTTG ACCCGCACAG CTTCCAAAGC TCCGCCCTTT
    CCCATACTAC GCTTTCCTAA AAGCATTGCT GTGTCCCAGG CTGCAGTAAA GCCCCCCCCT
    GAGCCCTCCT ATGATTTCTC TTCACCTGTC GACAGCTGCT CTTCTGACTT CACCTCGGGC
    TTGAGTGATG GCAATGACGG CCAATCAGAG AGGAGCAGGA TGGAGGCAAC GATGAGGGAA
    GCAACCAAAC AGTTTAGAAG GAGAGCCAGA GCTCGTCTCC GAATCATAGG GGTGTCTGAC
    AAGGTGGACC GAGTGGTGGA GTGTCAGCTG CAGACTCACA ACAACAAGAT GGTCACGTTT
    AAGTTTGACC TGGATGGAGA CAATCCCGAG GACATCGCAT CTGTGATGAT TCACAGAGAT
    TTTATCTTAC CGTCTGAGCG AGAAGGATTT ATCCTCCGCA TGTATGACAT CATCCAGAGG
    GCGGAGTCTA TGATACACCA GCATCATCCT GCAGTCTTGT ACCAGCCTTC TCAGCTGATG
    ACCTCTGCTC CCCAGTCAAA CTGCCTCTGC TTTCAACCTT TTCAGCCAAA GTCAGCAGTG
    ACTAACCTGT CCAGGACTTT GTCTTCCTCC TCCTTACCTG ACATAGATGA AAGTGACACC
    ACCCCCTCAA AGGCAATTGA CTTCAGCATT GACCCTGATG CTGTTCCCCC CGTGAGGCCG
    TTGAGGTCAC AGTCCTTCCA GACGTCATCA GCCTCTTCCT ATGAGCCCCC CCTTTACCCT
    TACCATTACC CCCATCCTGA ATCTACCACT CCCCCCCTCT CTCAGAATCT GCTTCCTTCT
    CATCTTTACT CCACCTATAC AGCCTCTTTC TCCACGCCCA GCTCCGCCCC CCAGATTCCA
    CGAGTCCACA GCAACCCCTC CCTCTTTACC TCCACACCAT CATCTTTAGC CCCTCCCTCT
    CCTAGGTGGC CACCCCCTGA CCAGCCTCTC TTCTCTTTGG CCAATGTACT GTCTGTGGCC
    ATGAGTGTGG CCCACAGCCT CATGCCCCCA CCCGGACATC CGAGTCAAGG CTTTCAACAC
    CAGGCTCCTC CCCCGCCTTT CCCCCAGTCC ACGTTCTCAC CCCCCATGTC TCCATCTCTA
    AAACGGAACC TTCAGACTTT GACAAACACC TCCACCGCTG AACCCCACCA ACCCCCCCAG
    GCCTATCACA CAGGCTTGGA GCCAGAAGTC CAAAAGACAG TGATGGACGG CGGAGCTGCA
    GATCAGTGTC CCGGTCCTGC CCGACCACGG CTCGGCTCGG CTCCCTCCTC CCAGGGTTCA
    GAGACCTCCG TTCTTCCCCG TCAGGCCAGC CTCTCAAATC CCAGAGGAGA GTTTTCAGAT
    TCCTCCACCT CGTCCTCAGC CTCCCCTCCT CCCCCATCGG GTTTCTACCC CAAAGTGTCC
    CCCCCAGTGT CCCCATCACG CTCCATCTCT CCTACGTTGA AAGCGGGGAA AACAGCCTTC
    ACTGTGGGCC GCTTCCAGGT GACCCCATCA ACACCCGTTT CTGCTGTCAG TCATCATCAG
    CCACGCCCCT TTAGTGATGC TTCACCCATC GCTCACTCCC CCCCTCCTAC TCAGAAGAAC
    CAATCAGAGA GCAGCTCAAG AAGCAGTTCA GAAGAAGACA GCCAATCAGA AATCAGCAAC
    CACACAGTCA TCAATACAAC ATATGGTTAC CGTGACAACC CCACCAGTCA AGACGAGAAG
    TGCATGAGCA GTCTTAAACT GGGAATGACT ATGTGGGAGG GGCCAGCCAA CAGCCAATCA
    CGTAGCCGTT CTGCTACTTA CAGCTCTGAC GAATCAGAGG GTGAGGAGAT GTGGGAGGAG
    CTACTGGAGC TTCGTAAAAG ACACTTGGTG GAGGTCCAGA ATCTACAGAT GAGTCAAAAA
    CAAGAAATGG AGGAGTTGTA CAGACGGAAG GGTAAAGCCC TACCACCTGG GATCGTCCCT
    CCAGCCGCCA TGCTGAACCA CCGCCAACGC CGCCTCTCCA AGACAGGAAA CTATCCTCTG
    GCACGCCGAA GCAGCCTCCA GAGACTAGAT CTGCCGCCCC CTGCAGGGAT CATGAGGAAG
    GGCTCGGTCA GCGGTAGCAG TAGTGGATCT CAAGACCGGA TCCTGAAAGG AGTGACGTTT
    GCTCCTGGTC ACAGCTGCAT GGTGAGCAAC AGAGGACCAG AACCAGCTCC ATACCCGGAT
    CTCTGTAGAA GTCAGGACCA GAATCACAGT CAGGACCAGA ATCACAGTCA GGACCAGGAC
    TACGACCAAA TTCACAGTCA GGACGAGGTT CAGGTCCAGG TCCAGGACCA GGGGCCTCAT
    TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa68004
    Clone ID Related Accession (Same CDS sequence) XM_020700651.1
    Accession Version XM_020700651.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1491408000000
    Organism Oryzias latipes(Japanese medaka)
    Product serine/threonine-protein kinase WNK4-like isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004090421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGACGACA AGGAGAAGCT CCGGGGGAAG TATAAAGACA ACGACGCCAT CGAGTTCCTG 
    TACGAACTCT ACAAGGATGT GCCAGAGGAG GTCGCCCAGG AGATGGTCAT TTTGGGATTT
    GTGTTCAAAG CAGACTACAA GGTGATTGCC AAAGCCATTC GGCATCGAGT GACAGCCATA
    AAGTATCAGC GGGAAAAACG GCGCCTCCTG CTGGAGGAAG GAGTGAACAG TCAGAAGCAA
    ACTGACATCG AGGAAGAGCT TCATCTTCAT CAACGAGCTG AGCTGTCCAA TCAGACGCTC
    CCAGTTTCAA CAGGAGCCAG AGGAGCGGCC AGTCAGGGGG ACTCTGCTGC ATCCACACAA
    GCTCCGCCCC TGACTGCAAC AGCACAAGCT CCGCCCCTGA CTGCATCAGC ACAAGCTCCG
    CCTCTGACTG CAACAGCACA AGCTCCGCCC CTGACTGCAT CAGCACAAGC TCCGCCCCTG
    ACTGCAACAG CACAAGCTCC GCCCCTGACT GCATCAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CACGCTCCTT CTCTAACTGC ATCGGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCCAC ACACGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGATTGCAT CCACACACTC TCCTTCCCTA ACTGCATCGG CACAAGCTCC ACCCCTGACT
    GCATCCACAC ACGCTCCTTC CCTAACTGCA TCGGCACAAG CTCCGCCCCT GACTGCATCG
    GCACAAACTC CGCCCCTGAC TGCATCGGCA CAAGCTCCGC CCCTGACTGC ATCAGCACAA
    GCTCCGCCCC TGACTGCATC CACACACGCT CCGCCCCTGA CTTTATCAGC ACAAGCTCCG
    CCCCTGACTG CATCAGCACA AGCTCCGCCC CTGACTGCAT CCACACAAGC TCCGCCCAGC
    AGCCGGGGGG ATTCCGGGAT CAACACTGTC TCCAGCAGAG CTGATGGCGA CACCGAGGAG
    GACGGTGCGA TCCGACTAGT CTCCCACCCC CCCACTGCCT CAGACCATGT GATTGAAACC
    ACGCCTCTTC TTGTTCCTAA CCCCACTCCT CCATCAGTAC CTCCACAGAC GGAGGTTCCT
    TCTGTTTTGA CCCGCACAGC TTCCAAAGCT CCGCCCTTTC CCATACTACG CTTTCCTAAA
    AGCATTGCTG TGTCCCAGGC TGCAGTAAAG CCCCCCCCTG AGCCCTCCTA TGATTTCTCT
    TCACCTGTCG ACAGCTGCTC TTCTGACTTC ACCTCGGGCT TGAGTGATGG CAATGACGGC
    CAATCAGAGA GGAGCAGGAT GGAGGCAACG ATGAGGGAAG CAACCAAACA GTTTAGAAGG
    AGAGCCAGAG CTCGTCTCCG AATCATAGGG GTGTCTGACA AGGTGGACCG AGTGGTGGAG
    TGTCAGCTGC AGACTCACAA CAACAAGATG GTCACGTTTA AGTTTGACCT GGATGGAGAC
    AATCCCGAGG ACATCGCATC TGTGATGATT CACAGAGATT TTATCTTACC GTCTGAGCGA
    GAAGGATTTA TCCTCCGCAT GTATGACATC ATCCAGAGGG CGGAGTCTAT GATACACCAG
    CATCATCCTG CAGTCTTGTA CCAGCCTTCT CAGCTGATGA CCTCTGCTCC CCAGTCAAAC
    TGCCTCTGCT TTCAACCTTT TCAGCCAAAG TCAGCAGTGA CTAACCTGTC CAGGACTTTG
    TCTTCCTCCT CCTTACCTGA CATAGATGAA AGTGACACCA CCCCCTCAAA GGCAATTGAC
    TTCAGCATTG ACCCTGATGC TGTTCCCCCC GTGAGGCCGT TGAGGTCACA GTCCTTCCAG
    ACGTCATCAG CCTCTTCCTA TGAGCCCCCC CTTTACCCTT ACCATTACCC CCATCCTGAA
    TCTACCACTC CCCCCCTCTC TCAGAATCTG CTTCCTTCTC ATCTTTACTC CACCTATACA
    GCCTCTTTCT CCACGCCCAG CTCCGCCCCC CAGATTCCAC GAGTCCACAG CAACCCCTCC
    CTCTTTACCT CCACACCATC ATCTTTAGCC CCTCCCTCTC CTAGGTGGCC ACCCCCTGAC
    CAGCCTCTCT TCTCTTTGGC CAATGTACTG TCTGTGGCCA TGAGTGTGGC CCACAGCCTC
    ATGCCCCCAC CCGGACATCC GAGTCAAGGC TTTCAACACC AGGCTCCTCC CCCGCCTTTC
    CCCCAGTCCA CGTTCTCACC CCCCATGTCT CCATCTCTAA AACGGAACCT TCAGACTTTG
    ACAAACACCT CCACCGCTGA ACCCCACCAA CCCCCCCAGG CCTATCACAC AGGCTTGGAG
    CCAGAAGTCC AAAAGACAGT GATGGACGGC GGAGCTGCAG ATCAGTGTCC CGGTCCTGCC
    CGACCACGGC TCGGCTCGGC TCCCTCCTCC CAGGGTTCAG AGACCTCCGT TCTTCCCCGT
    CAGGCCAGCC TCTCAAATCC CAGAGGAGAG TTTTCAGATT CCTCCACCTC GTCCTCAGCC
    TCCCCTCCTC CCCCATCGGG TTTCTACCCC AAAGTGTCCC CCCCAGTGTC CCCATCACGC
    TCCATCTCTC CTACGTTGAA AGCGGGGAAA ACAGCCTTCA CTGTGGGCCG CTTCCAGGTG
    ACCCCATCAA CACCCGTTTC TGCTGTCAGT CATCATCAGC CACGCCCCTT TAGTGATGCT
    TCACCCATCG CTCACTCCCC CCCTCCTACT CAGAAGAACC AATCAGAGAG CAGCTCAAGA
    AGCAGTTCAG AAGAAGACAG CCAATCAGAA ATCAGCAACC ACACAGTCAT CAATACAACA
    TATGGTTACC GTGACAACCC CACCAGTCAA GACGAGAAGT GCATGAGCAG TCTTAAACTG
    GGAATGACTA TGTGGGAGGG GCCAGCCAAC AGCCAATCAC GTAGCCGTTC TGCTACTTAC
    AGCTCTGACG AATCAGAGGG TGAGGAGATG TGGGAGGAGC TACTGGAGCT TCGTAAAAGA
    CACTTGGTGG AGGTCCAGAA TCTACAGATG AGTCAAAAAC AAGAAATGGA GGAGTTGTAC
    AGACGGAAGG GTAAAGCCCT ACCACCTGGG ATCGTCCCTC CAGCCGCCAT GCTGAACCAC
    CGCCAACGCC GCCTCTCCAA GACAGGAAAC TATCCTCTGG CACGCCGAAG CAGCCTCCAG
    AGACTAGATC TGCCGCCCCC TGCAGGGATC ATGAGGAAGG GCTCGGTCAG CGGTAGCAGT
    AGTGGATCTC AAGACCGGAT CCTGAAAGGA GTGACGTTTG CTCCTGGTCA CAGCTGCATG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020556310.1
    CDS204..3566
    Translation

    Target ORF information:

    RefSeq Version XM_020700651.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes serine/threonine-protein kinase WNK4-like (LOC101162572), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_020700651.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGACGACA AGGAGAAGCT CCGGGGGAAG TATAAAGACA ACGACGCCAT CGAGTTCCTG 
    TACGAACTCT ACAAGGATGT GCCAGAGGAG GTCGCCCAGG AGATGGTCAT TTTGGGATTT
    GTGTTCAAAG CAGACTACAA GGTGATTGCC AAAGCCATTC GGCATCGAGT GACAGCCATA
    AAGTATCAGC GGGAAAAACG GCGCCTCCTG CTGGAGGAAG GAGTGAACAG TCAGAAGCAA
    ACTGACATCG AGGAAGAGCT TCATCTTCAT CAACGAGCTG AGCTGTCCAA TCAGACGCTC
    CCAGTTTCAA CAGGAGCCAG AGGAGCGGCC AGTCAGGGGG ACTCTGCTGC ATCCACACAA
    GCTCCGCCCC TGACTGCAAC AGCACAAGCT CCGCCCCTGA CTGCATCAGC ACAAGCTCCG
    CCTCTGACTG CAACAGCACA AGCTCCGCCC CTGACTGCAT CAGCACAAGC TCCGCCCCTG
    ACTGCAACAG CACAAGCTCC GCCCCTGACT GCATCAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CACGCTCCTT CTCTAACTGC ATCGGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCCAC ACACGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGATTGCAT CCACACACTC TCCTTCCCTA ACTGCATCGG CACAAGCTCC ACCCCTGACT
    GCATCCACAC ACGCTCCTTC CCTAACTGCA TCGGCACAAG CTCCGCCCCT GACTGCATCG
    GCACAAACTC CGCCCCTGAC TGCATCGGCA CAAGCTCCGC CCCTGACTGC ATCAGCACAA
    GCTCCGCCCC TGACTGCATC CACACACGCT CCGCCCCTGA CTTTATCAGC ACAAGCTCCG
    CCCCTGACTG CATCAGCACA AGCTCCGCCC CTGACTGCAT CCACACAAGC TCCGCCCAGC
    AGCCGGGGGG ATTCCGGGAT CAACACTGTC TCCAGCAGAG CTGATGGCGA CACCGAGGAG
    GACGGTGCGA TCCGACTAGT CTCCCACCCC CCCACTGCCT CAGACCATGT GATTGAAACC
    ACGCCTCTTC TTGTTCCTAA CCCCACTCCT CCATCAGTAC CTCCACAGAC GGAGGTTCCT
    TCTGTTTTGA CCCGCACAGC TTCCAAAGCT CCGCCCTTTC CCATACTACG CTTTCCTAAA
    AGCATTGCTG TGTCCCAGGC TGCAGTAAAG CCCCCCCCTG AGCCCTCCTA TGATTTCTCT
    TCACCTGTCG ACAGCTGCTC TTCTGACTTC ACCTCGGGCT TGAGTGATGG CAATGACGGC
    CAATCAGAGA GGAGCAGGAT GGAGGCAACG ATGAGGGAAG CAACCAAACA GTTTAGAAGG
    AGAGCCAGAG CTCGTCTCCG AATCATAGGG GTGTCTGACA AGGTGGACCG AGTGGTGGAG
    TGTCAGCTGC AGACTCACAA CAACAAGATG GTCACGTTTA AGTTTGACCT GGATGGAGAC
    AATCCCGAGG ACATCGCATC TGTGATGATT CACAGAGATT TTATCTTACC GTCTGAGCGA
    GAAGGATTTA TCCTCCGCAT GTATGACATC ATCCAGAGGG CGGAGTCTAT GATACACCAG
    CATCATCCTG CAGTCTTGTA CCAGCCTTCT CAGCTGATGA CCTCTGCTCC CCAGTCAAAC
    TGCCTCTGCT TTCAACCTTT TCAGCCAAAG TCAGCAGTGA CTAACCTGTC CAGGACTTTG
    TCTTCCTCCT CCTTACCTGA CATAGATGAA AGTGACACCA CCCCCTCAAA GGCAATTGAC
    TTCAGCATTG ACCCTGATGC TGTTCCCCCC GTGAGGCCGT TGAGGTCACA GTCCTTCCAG
    ACGTCATCAG CCTCTTCCTA TGAGCCCCCC CTTTACCCTT ACCATTACCC CCATCCTGAA
    TCTACCACTC CCCCCCTCTC TCAGAATCTG CTTCCTTCTC ATCTTTACTC CACCTATACA
    GCCTCTTTCT CCACGCCCAG CTCCGCCCCC CAGATTCCAC GAGTCCACAG CAACCCCTCC
    CTCTTTACCT CCACACCATC ATCTTTAGCC CCTCCCTCTC CTAGGTGGCC ACCCCCTGAC
    CAGCCTCTCT TCTCTTTGGC CAATGTACTG TCTGTGGCCA TGAGTGTGGC CCACAGCCTC
    ATGCCCCCAC CCGGACATCC GAGTCAAGGC TTTCAACACC AGGCTCCTCC CCCGCCTTTC
    CCCCAGTCCA CGTTCTCACC CCCCATGTCT CCATCTCTAA AACGGAACCT TCAGACTTTG
    ACAAACACCT CCACCGCTGA ACCCCACCAA CCCCCCCAGG CCTATCACAC AGGCTTGGAG
    CCAGAAGTCC AAAAGACAGT GATGGACGGC GGAGCTGCAG ATCAGTGTCC CGGTCCTGCC
    CGACCACGGC TCGGCTCGGC TCCCTCCTCC CAGGGTTCAG AGACCTCCGT TCTTCCCCGT
    CAGGCCAGCC TCTCAAATCC CAGAGGAGAG TTTTCAGATT CCTCCACCTC GTCCTCAGCC
    TCCCCTCCTC CCCCATCGGG TTTCTACCCC AAAGTGTCCC CCCCAGTGTC CCCATCACGC
    TCCATCTCTC CTACGTTGAA AGCGGGGAAA ACAGCCTTCA CTGTGGGCCG CTTCCAGGTG
    ACCCCATCAA CACCCGTTTC TGCTGTCAGT CATCATCAGC CACGCCCCTT TAGTGATGCT
    TCACCCATCG CTCACTCCCC CCCTCCTACT CAGAAGAACC AATCAGAGAG CAGCTCAAGA
    AGCAGTTCAG AAGAAGACAG CCAATCAGAA ATCAGCAACC ACACAGTCAT CAATACAACA
    TATGGTTACC GTGACAACCC CACCAGTCAA GACGAGAAGT GCATGAGCAG TCTTAAACTG
    GGAATGACTA TGTGGGAGGG GCCAGCCAAC AGCCAATCAC GTAGCCGTTC TGCTACTTAC
    AGCTCTGACG AATCAGAGGG TGAGGAGATG TGGGAGGAGC TACTGGAGCT TCGTAAAAGA
    CACTTGGTGG AGGTCCAGAA TCTACAGATG AGTCAAAAAC AAGAAATGGA GGAGTTGTAC
    AGACGGAAGG GTAAAGCCCT ACCACCTGGG ATCGTCCCTC CAGCCGCCAT GCTGAACCAC
    CGCCAACGCC GCCTCTCCAA GACAGGAAAC TATCCTCTGG CACGCCGAAG CAGCCTCCAG
    AGACTAGATC TGCCGCCCCC TGCAGGGATC ATGAGGAAGG GCTCGGTCAG CGGTAGCAGT
    AGTGGATCTC AAGACCGGAT CCTGAAAGGA GTGACGTTTG CTCCTGGTCA CAGCTGCATG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa117513
    Clone ID Related Accession (Same CDS sequence) XM_023957160.1
    Accession Version XM_023957160.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4620bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1518537600000
    Organism Oryzias latipes(Japanese medaka)
    Product serine/threonine-protein kinase WNK1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019866.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGCACT CCGCTGAAGA TGAAGAAGAG GAGAACTCTG CTCTTCATCT CAAGGACTCC 
    GACCAATCAG AGGCTTCCGA TCCACACACA GGTCATGATG TCAGCTCAGA CTCTGCCGAT
    GAAATGGGCA TCAATCTCTC AGAATTAAAC AGTGAGATAT TACCATGGCA ACGCACCCAG
    GAGGAGGAGC ACGACGAAGA GGAAATGAAC GCTGTGGCGT CGTCGCCTGA TGGGCGGTTC
    CTGAAGTTCA ACATCGAGAT CGGGAGGGGC TCCTTCAAAA CCGTTTACAA AGGGCTGGAC
    ACGGAGACCA CAGTGGAGGT GGCCTGGTGC GAGCTCCAGA CCCACCGGCT GAGCAGGGCC
    GAGCGTCAGC GTTTCAGCGA GGAGGTGGAG ATGCTCAAAG CGCTTCAGCA CCCCAACATC
    GTCCGTTTCT TCGATTCCTG GAAGTCCGCG CTGAAGGGCC ACAAATGCAC CATCCTGGTG
    ACGGAGCTCA TGACGTCTGG AACGCTGAAG ATGTACCTGC GCAGGTTCCG TCAGATGAAG
    CTGAAGCTGC TGCAGCGCTG GAGCTTCCAG GTCCTGCGGG GGCTTCACTT CCTGCACTCA
    CGTTCCCCCC CCATCATACA CCGTGACCTC AAATGTGACA ACATCTTCAT CACGGGTCCC
    TCCGCCTCCG TCAAAATCGG AGACCTGGGC CTGGCAACGC TGAAGAAGGC CTCGTTTGCT
    AAGAGCGTGA TCGGAACGCC GGAGTTCATG GCTCCAGAGA TGTACGAGGA GAAGTACGAC
    GAGGCTGTGG ACGTGTACGC CTTCGGCATG TGCATGCTGG AGATGGCTAC GTCCGAGTAT
    CCGTACCACG AGTGTCACAG CGCTGCCCAG ATCTACCGCA AGGTCACCAA TGGAATCAAA
    CCGGACAGTT TCTTCAAAGT GGAAGTTCCA GAACTGAAAG AAATCATTGA AGGCTGCATT
    CGGACCAGGA GCAGTGAAAG GTTTACAGTC CAGGACCTCT TGGAGCACAG CTTCTTTCAG
    GAGAACACCG GCGTCCATGT GGAGCTGGCA GAGGAAGACG ACGGCTCCAA GGCGGCGCTG
    AAGCTGTGGC TCCGGATGGA CGACAAGGAG AAGCTCCGGG GGAAGTATAA AGACAACGAC
    GCCATCGAGT TCCTGTACGA ACTCTACAAG GATGTGCCAG AGGAGGTCGC CCAGGAGATG
    GTCATTTTGG GATTTGTGTT CAAAGCAGAC TACAAGGTGA TTGCCAAAGC CATTCGGCAT
    CGAGTGACAG CCATAAAGTA TCAGCGGGAA AAACGGCGCC TCCTGCTGGA GGAAGGAGTG
    AACAGTCAGA AGCAAACTGA CATCGAGGAA GAGCTTCATC TTCATCAACG AGCTGAGCTG
    TCCAATCAGA CGCTCCCAGT TTCAACAGGA GCCAGAGGAG CGGCCAGTCA GGGGGACTCT
    GCTGCATCCA CACAAGCTCC GCCCCTGACT GCAACAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCTCT GACTGCAACA GCACAAGCTC CGCCCCTGAC TGCATCAGCA
    CAAGCTCCGC CCCTGACTGC AACAGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCAGC ACAAGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGACTGCAT CCACACACGC TCCTTCTCTA ACTGCATCGG CACAAGCTCC GCCCCTGACT
    GCATCAGCAC AAGCTCCGCC CCTGACTGCA TCCACACACG CTCCGCCCCT GACTGCATCA
    GCACAAGCTC CGCCCCTGAT TGCATCCACA CACTCTCCTT CCCTAACTGC ATCGGCACAA
    GCTCCACCCC TGACTGCATC CACACACGCT CCTTCCCTAA CTGCATCGGC ACAAGCTCCG
    CCCCTGACTG CATCGGCACA AACTCCGCCC CTGACTGCAT CGGCACAAGC TCCGCCCCTG
    ACTGCATCAG CACAAGCTCC GCCCCTGACT GCATCCACAC ACGCTCCGCC CCTGACTTTA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CAAGCTCCGC CCAGCAGCCG GGGGGATTCC GGGATCAACA CTGTCTCCAG CAGAGCTGAT
    GGCGACACCG AGGAGGACGG TGCGATCCGA CTAGTCTCCC ACCCCCCCAC TGCCTCAGAC
    CATGTGATTG AAACCACGCC TCTTCTTGTT CCTAACCCCA CTCCTCCATC AGTACCTCCA
    CAGACGGAGG TTCCTTCTGT TTTGACCCGC ACAGCTTCCA AAGCTCCGCC CTTTCCCATA
    CTACGCTTTC CTAAAAGCAT TGCTGTGTCC CAGGCTGCAG TAAAGCCCCC CCCTGAGCCC
    TCCTATGATT TCTCTTCACC TGTCGACAGC TGCTCTTCTG ACTTCACCTC GGGCTTGAGT
    GATGGCAATG ACGGCCAATC AGAGAGGAGC AGGATGGAGG CAACGATGAG GGAAGCAACC
    AAACAGTTTA GAAGGAGAGC CAGAGCTCGT CTCCGAATCA TAGGGGTGTC TGACAAGGTG
    GACCGAGTGG TGGAGTGTCA GCTGCAGACT CACAACAACA AGATGGTCAC GTTTAAGTTT
    GACCTGGATG GAGACAATCC CGAGGACATC GCATCTGTGA TGATTCACAG AGATTTTATC
    TTACCGTCTG AGCGAGAAGG ATTTATCCTC CGCATGTATG ACATCATCCA GAGGGCGGAG
    TCTATGATAC ACCAGCATCA TCCTGCAGTC TTGTACCAGC CTTCTCAGCT GATGACCTCT
    GCTCCCCAGT CAAACTGCCT CTGCTTTCAA CCTTTTCAGC CAAAGTCAGC AGTGACTAAC
    CTGTCCAGGA CTTTGTCTTC CTCCTCCTTA CCTGACATAG ATGAAAGTGA CACCACCCCC
    TCAAAGGCAA TTGACTTCAG CATTGACCCT GATGCTGTTC CCCCCGTGAG GCCGTTGAGG
    TCACAGTCCT TCCAGACGTC ATCAGCCTCT TCCTATGAGC CCCCCCTTTA CCCTTACCAT
    TACCCCCATC CTGAATCTAC CACTCCCCCC CTCTCTCAGA ATCTGCTTCC TTCTCATCTT
    TACTCCACCT ATACAGCCTC TTTCTCCACG CCCAGCTCCG CCCCCCAGAT TCCACGAGTC
    CACAGCAACC CCTCCCTCTT TACCTCCACA CCATCATCTT TAGCCCCTCC CTCTCCTAGG
    TGGCCACCCC CTGACCAGCC TCTCTTCTCT TTGGCCAATG TACTGTCTGT GGCCATGAGT
    GTGGCCCACA GCCTCATGCC CCCACCCGGA CATCCGAGTC AAGGCTTTCA ACACCAGGCT
    CCTCCCCCGC CTTTCCCCCA GTCCACGTTC TCACCCCCCA TGTCTCCATC TCTAAAACGG
    AACCTTCAGA CTTTGACAAA CACCTCCACC GCTGAACCCC ACCAACCCCC CCAGGCCTAT
    CACACAGGCT TGGAGCCAGA AGTCCAAAAG ACAGTGATGG ACGGCGGAGC TGCAGATCAG
    TGTCCCGGTC CTGCCCGACC ACGGCTCGGC TCGGCTCCCT CCTCCCAGGG TTCAGAGACC
    TCCGTTCTTC CCCGTCAGGC CAGCCTCTCA AATCCCAGAG GAGAGTTTTC AGATTCCTCC
    ACCTCGTCCT CAGCCTCCCC TCCTCCCCCA TCGGGTTTCT ACCCCAAAGT GTCCCCCCCA
    GTGTCCCCAT CACGCTCCAT CTCTCCTACG TTGAAAGCGG GGAAAACAGC CTTCACTGTG
    GGCCGCTTCC AGGTGACCCC ATCAACACCC GTTTCTGCTG TCAGTCATCA TCAGCCACGC
    CCCTTTAGTG ATGCTTCACC CATCGCTCAC TCCCCCCCTC CTACTCAGAA GAACCAATCA
    GAGAGCAGCT CAAGAAGCAG TTCAGAAGAA GACAGCCAAT CAGAAATCAG CAACCACACA
    GTCATCAATA CAACATATGG TTACCGTGAC AACCCCACCA GTCAAGACGA GAAGTGCATG
    AGCAGTCTTA AACTGGGAAT GACTATGTGG GAGGGGCCAG CCAACAGCCA ATCACGTAGC
    CGTTCTGCTA CTTACAGCTC TGACGAATCA GAGGGTGAGG AGATGTGGGA GGAGCTACTG
    GAGCTTCGTA AAAGACACTT GGTGGAGGTC CAGAATCTAC AGATGAGTCA AAAACAAGAA
    ATGGAGGAGT TGTACAGACG GAAGGGTAAA GCCCTACCAC CTGGGATCGT CCCTCCAGCC
    GCCATGCTGA ACCACCGCCA ACGCCGCCTC TCCAAGACAG GAAACTATCC TCTGGCACGC
    CGAAGCAGCC TCCAGAGACT AGATCTGCCG CCCCCTGCAG GGATCATGAG GAAGGGCTCG
    GTCAGCGGTA GCAGTAGTGG ATCTCAAGAC CGGATCCTGA AAGGAGTGAC GTTTGCTCCT
    GGTCACAGCT GCATGGTGAG CAACAGAGGA CCAGAACCAG CTCCATACCC GGATCTCTGT
    AGAAGTCAGG ACCAGAATCA CAGTCAGGAC CAGAATCACA GTCAGGACCA GGACTACGAC
    CAAATTCACA GTCAGGACGA GGTTCAGGTC CAGGTCCAGG ACCAGGGGCC TCATTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023812928.1
    CDS917..5536
    Translation

    Target ORF information:

    RefSeq Version XM_023957160.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes serine/threonine-protein kinase WNK1 (LOC101162572), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023957160.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGCACT CCGCTGAAGA TGAAGAAGAG GAGAACTCTG CTCTTCATCT CAAGGACTCC 
    GACCAATCAG AGGCTTCCGA TCCACACACA GGTCATGATG TCAGCTCAGA CTCTGCCGAT
    GAAATGGGCA TCAATCTCTC AGAATTAAAC AGTGAGATAT TACCATGGCA ACGCACCCAG
    GAGGAGGAGC ACGACGAAGA GGAAATGAAC GCTGTGGCGT CGTCGCCTGA TGGGCGGTTC
    CTGAAGTTCA ACATCGAGAT CGGGAGGGGC TCCTTCAAAA CCGTTTACAA AGGGCTGGAC
    ACGGAGACCA CAGTGGAGGT GGCCTGGTGC GAGCTCCAGA CCCACCGGCT GAGCAGGGCC
    GAGCGTCAGC GTTTCAGCGA GGAGGTGGAG ATGCTCAAAG CGCTTCAGCA CCCCAACATC
    GTCCGTTTCT TCGATTCCTG GAAGTCCGCG CTGAAGGGCC ACAAATGCAC CATCCTGGTG
    ACGGAGCTCA TGACGTCTGG AACGCTGAAG ATGTACCTGC GCAGGTTCCG TCAGATGAAG
    CTGAAGCTGC TGCAGCGCTG GAGCTTCCAG GTCCTGCGGG GGCTTCACTT CCTGCACTCA
    CGTTCCCCCC CCATCATACA CCGTGACCTC AAATGTGACA ACATCTTCAT CACGGGTCCC
    TCCGCCTCCG TCAAAATCGG AGACCTGGGC CTGGCAACGC TGAAGAAGGC CTCGTTTGCT
    AAGAGCGTGA TCGGAACGCC GGAGTTCATG GCTCCAGAGA TGTACGAGGA GAAGTACGAC
    GAGGCTGTGG ACGTGTACGC CTTCGGCATG TGCATGCTGG AGATGGCTAC GTCCGAGTAT
    CCGTACCACG AGTGTCACAG CGCTGCCCAG ATCTACCGCA AGGTCACCAA TGGAATCAAA
    CCGGACAGTT TCTTCAAAGT GGAAGTTCCA GAACTGAAAG AAATCATTGA AGGCTGCATT
    CGGACCAGGA GCAGTGAAAG GTTTACAGTC CAGGACCTCT TGGAGCACAG CTTCTTTCAG
    GAGAACACCG GCGTCCATGT GGAGCTGGCA GAGGAAGACG ACGGCTCCAA GGCGGCGCTG
    AAGCTGTGGC TCCGGATGGA CGACAAGGAG AAGCTCCGGG GGAAGTATAA AGACAACGAC
    GCCATCGAGT TCCTGTACGA ACTCTACAAG GATGTGCCAG AGGAGGTCGC CCAGGAGATG
    GTCATTTTGG GATTTGTGTT CAAAGCAGAC TACAAGGTGA TTGCCAAAGC CATTCGGCAT
    CGAGTGACAG CCATAAAGTA TCAGCGGGAA AAACGGCGCC TCCTGCTGGA GGAAGGAGTG
    AACAGTCAGA AGCAAACTGA CATCGAGGAA GAGCTTCATC TTCATCAACG AGCTGAGCTG
    TCCAATCAGA CGCTCCCAGT TTCAACAGGA GCCAGAGGAG CGGCCAGTCA GGGGGACTCT
    GCTGCATCCA CACAAGCTCC GCCCCTGACT GCAACAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCTCT GACTGCAACA GCACAAGCTC CGCCCCTGAC TGCATCAGCA
    CAAGCTCCGC CCCTGACTGC AACAGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCAGC ACAAGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGACTGCAT CCACACACGC TCCTTCTCTA ACTGCATCGG CACAAGCTCC GCCCCTGACT
    GCATCAGCAC AAGCTCCGCC CCTGACTGCA TCCACACACG CTCCGCCCCT GACTGCATCA
    GCACAAGCTC CGCCCCTGAT TGCATCCACA CACTCTCCTT CCCTAACTGC ATCGGCACAA
    GCTCCACCCC TGACTGCATC CACACACGCT CCTTCCCTAA CTGCATCGGC ACAAGCTCCG
    CCCCTGACTG CATCGGCACA AACTCCGCCC CTGACTGCAT CGGCACAAGC TCCGCCCCTG
    ACTGCATCAG CACAAGCTCC GCCCCTGACT GCATCCACAC ACGCTCCGCC CCTGACTTTA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CAAGCTCCGC CCAGCAGCCG GGGGGATTCC GGGATCAACA CTGTCTCCAG CAGAGCTGAT
    GGCGACACCG AGGAGGACGG TGCGATCCGA CTAGTCTCCC ACCCCCCCAC TGCCTCAGAC
    CATGTGATTG AAACCACGCC TCTTCTTGTT CCTAACCCCA CTCCTCCATC AGTACCTCCA
    CAGACGGAGG TTCCTTCTGT TTTGACCCGC ACAGCTTCCA AAGCTCCGCC CTTTCCCATA
    CTACGCTTTC CTAAAAGCAT TGCTGTGTCC CAGGCTGCAG TAAAGCCCCC CCCTGAGCCC
    TCCTATGATT TCTCTTCACC TGTCGACAGC TGCTCTTCTG ACTTCACCTC GGGCTTGAGT
    GATGGCAATG ACGGCCAATC AGAGAGGAGC AGGATGGAGG CAACGATGAG GGAAGCAACC
    AAACAGTTTA GAAGGAGAGC CAGAGCTCGT CTCCGAATCA TAGGGGTGTC TGACAAGGTG
    GACCGAGTGG TGGAGTGTCA GCTGCAGACT CACAACAACA AGATGGTCAC GTTTAAGTTT
    GACCTGGATG GAGACAATCC CGAGGACATC GCATCTGTGA TGATTCACAG AGATTTTATC
    TTACCGTCTG AGCGAGAAGG ATTTATCCTC CGCATGTATG ACATCATCCA GAGGGCGGAG
    TCTATGATAC ACCAGCATCA TCCTGCAGTC TTGTACCAGC CTTCTCAGCT GATGACCTCT
    GCTCCCCAGT CAAACTGCCT CTGCTTTCAA CCTTTTCAGC CAAAGTCAGC AGTGACTAAC
    CTGTCCAGGA CTTTGTCTTC CTCCTCCTTA CCTGACATAG ATGAAAGTGA CACCACCCCC
    TCAAAGGCAA TTGACTTCAG CATTGACCCT GATGCTGTTC CCCCCGTGAG GCCGTTGAGG
    TCACAGTCCT TCCAGACGTC ATCAGCCTCT TCCTATGAGC CCCCCCTTTA CCCTTACCAT
    TACCCCCATC CTGAATCTAC CACTCCCCCC CTCTCTCAGA ATCTGCTTCC TTCTCATCTT
    TACTCCACCT ATACAGCCTC TTTCTCCACG CCCAGCTCCG CCCCCCAGAT TCCACGAGTC
    CACAGCAACC CCTCCCTCTT TACCTCCACA CCATCATCTT TAGCCCCTCC CTCTCCTAGG
    TGGCCACCCC CTGACCAGCC TCTCTTCTCT TTGGCCAATG TACTGTCTGT GGCCATGAGT
    GTGGCCCACA GCCTCATGCC CCCACCCGGA CATCCGAGTC AAGGCTTTCA ACACCAGGCT
    CCTCCCCCGC CTTTCCCCCA GTCCACGTTC TCACCCCCCA TGTCTCCATC TCTAAAACGG
    AACCTTCAGA CTTTGACAAA CACCTCCACC GCTGAACCCC ACCAACCCCC CCAGGCCTAT
    CACACAGGCT TGGAGCCAGA AGTCCAAAAG ACAGTGATGG ACGGCGGAGC TGCAGATCAG
    TGTCCCGGTC CTGCCCGACC ACGGCTCGGC TCGGCTCCCT CCTCCCAGGG TTCAGAGACC
    TCCGTTCTTC CCCGTCAGGC CAGCCTCTCA AATCCCAGAG GAGAGTTTTC AGATTCCTCC
    ACCTCGTCCT CAGCCTCCCC TCCTCCCCCA TCGGGTTTCT ACCCCAAAGT GTCCCCCCCA
    GTGTCCCCAT CACGCTCCAT CTCTCCTACG TTGAAAGCGG GGAAAACAGC CTTCACTGTG
    GGCCGCTTCC AGGTGACCCC ATCAACACCC GTTTCTGCTG TCAGTCATCA TCAGCCACGC
    CCCTTTAGTG ATGCTTCACC CATCGCTCAC TCCCCCCCTC CTACTCAGAA GAACCAATCA
    GAGAGCAGCT CAAGAAGCAG TTCAGAAGAA GACAGCCAAT CAGAAATCAG CAACCACACA
    GTCATCAATA CAACATATGG TTACCGTGAC AACCCCACCA GTCAAGACGA GAAGTGCATG
    AGCAGTCTTA AACTGGGAAT GACTATGTGG GAGGGGCCAG CCAACAGCCA ATCACGTAGC
    CGTTCTGCTA CTTACAGCTC TGACGAATCA GAGGGTGAGG AGATGTGGGA GGAGCTACTG
    GAGCTTCGTA AAAGACACTT GGTGGAGGTC CAGAATCTAC AGATGAGTCA AAAACAAGAA
    ATGGAGGAGT TGTACAGACG GAAGGGTAAA GCCCTACCAC CTGGGATCGT CCCTCCAGCC
    GCCATGCTGA ACCACCGCCA ACGCCGCCTC TCCAAGACAG GAAACTATCC TCTGGCACGC
    CGAAGCAGCC TCCAGAGACT AGATCTGCCG CCCCCTGCAG GGATCATGAG GAAGGGCTCG
    GTCAGCGGTA GCAGTAGTGG ATCTCAAGAC CGGATCCTGA AAGGAGTGAC GTTTGCTCCT
    GGTCACAGCT GCATGGTGAG CAACAGAGGA CCAGAACCAG CTCCATACCC GGATCTCTGT
    AGAAGTCAGG ACCAGAATCA CAGTCAGGAC CAGAATCACA GTCAGGACCA GGACTACGAC
    CAAATTCACA GTCAGGACGA GGTTCAGGTC CAGGTCCAGG ACCAGGGGCC TCATTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa117514
    Clone ID Related Accession (Same CDS sequence) XM_023957161.1
    Accession Version XM_023957161.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4617bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1518537600000
    Organism Oryzias latipes(Japanese medaka)
    Product serine/threonine-protein kinase WNK1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019866.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGCACT CCGCTGAAGA TGAAGAAGAG GAGAACTCTG CTCTTCATCT CAAGGACTCC 
    GACCAATCAG AGGCTTCCGA TCCACACACA GGTCATGATG TCAGCTCAGA CTCTGCCGAT
    GAAATGGGCA TCAATCTCTC AGAATTAAAC AGTGAGATAT TACCATGGCA ACGCACCCAG
    GAGGAGGAGC ACGACGAAGA GGAAATGAAC GCTGTGGCGT CGTCGCCTGA TGGGCGGTTC
    CTGAAGTTCA ACATCGAGAT CGGGAGGGGC TCCTTCAAAA CCGTTTACAA AGGGCTGGAC
    ACGGAGACCA CAGTGGAGGT GGCCTGGTGC GAGCTCCAGA CCCACCGGCT GAGCAGGGCC
    GAGCGTCAGC GTTTCAGCGA GGAGGTGGAG ATGCTCAAAG CGCTTCAGCA CCCCAACATC
    GTCCGTTTCT TCGATTCCTG GAAGTCCGCG CTGAAGGGCC ACAAATGCAC CATCCTGGTG
    ACGGAGCTCA TGACGTCTGG AACGCTGAAG ATGTACCTGC GCAGGTTCCG TCAGATGAAG
    CTGAAGCTGC TGCAGCGCTG GAGCTTCCAG GTCCTGCGGG GGCTTCACTT CCTGCACTCA
    CGTTCCCCCC CCATCATACA CCGTGACCTC AAATGTGACA ACATCTTCAT CACGGGTCCC
    TCCGCCTCCG TCAAAATCGG AGACCTGGGC CTGGCAACGC TGAAGAAGGC CTCGTTTGCT
    AAGAGCGTGA TCGGAACGCC GGAGTTCATG GCTCCAGAGA TGTACGAGGA GAAGTACGAC
    GAGGCTGTGG ACGTGTACGC CTTCGGCATG TGCATGCTGG AGATGGCTAC GTCCGAGTAT
    CCGTACCACG AGTGTCACAG CGCTGCCCAG ATCTACCGCA AGGTCACCAA TGGAATCAAA
    CCGGACAGTT TCTTCAAAGT GGAAGTTCCA GAACTGAAAG AAATCATTGA AGGCTGCATT
    CGGACCAGGA GCAGTGAAAG GTTTACAGTC CAGGACCTCT TGGAGCACAG CTTCTTTCAG
    GAGAACACCG GCGTCCATGT GGAGCTGGCA GAGGAAGACG ACGGCTCCAA GGCGGCGCTG
    AAGCTGTGGC TCCGGATGGA CGACAAGGAG AAGCTCCGGG GGAAGTATAA AGACAACGAC
    GCCATCGAGT TCCTGTACGA ACTCTACAAG GATGTGCCAG AGGAGGTCGC CCAGGAGATG
    GTCATTTTGG GATTTGTGTT CAAAGCAGAC TACAAGGTGA TTGCCAAAGC CATTCGGCAT
    CGAGTGACAG CCATAAAGTA TCAGCGGGAA AAACGGCGCC TCCTGCTGGA GGAAGGAGTG
    AACAGTCAGA AGCAAACTGA CATCGAGGAA GAGCTTCATC TTCATCAACG AGCTGAGCTG
    TCCAATCAGA CGCTCCCAGT TTCAACAGGA GCCAGAGGAG CGGCCAGTCA GGGGGACTCT
    GCTGCATCCA CACAAGCTCC GCCCCTGACT GCAACAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCTCT GACTGCAACA GCACAAGCTC CGCCCCTGAC TGCATCAGCA
    CAAGCTCCGC CCCTGACTGC AACAGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCAGC ACAAGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGACTGCAT CCACACACGC TCCTTCTCTA ACTGCATCGG CACAAGCTCC GCCCCTGACT
    GCATCAGCAC AAGCTCCGCC CCTGACTGCA TCCACACACG CTCCGCCCCT GACTGCATCA
    GCACAAGCTC CGCCCCTGAT TGCATCCACA CACTCTCCTT CCCTAACTGC ATCGGCACAA
    GCTCCACCCC TGACTGCATC CACACACGCT CCTTCCCTAA CTGCATCGGC ACAAGCTCCG
    CCCCTGACTG CATCGGCACA AACTCCGCCC CTGACTGCAT CGGCACAAGC TCCGCCCCTG
    ACTGCATCAG CACAAGCTCC GCCCCTGACT GCATCCACAC ACGCTCCGCC CCTGACTTTA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CAAGCTCCGC CCAGCAGCCG GGGGGATTCC GGGATCAACA CTGTCTCCAG CAGAGCTGAT
    GGCGACACCG AGGAGGACGG TGCGATCCGA CTAGTCTCCC ACCCCCCCAC TGCCTACCAT
    GTGATTGAAA CCACGCCTCT TCTTGTTCCT AACCCCACTC CTCCATCAGT ACCTCCACAG
    ACGGAGGTTC CTTCTGTTTT GACCCGCACA GCTTCCAAAG CTCCGCCCTT TCCCATACTA
    CGCTTTCCTA AAAGCATTGC TGTGTCCCAG GCTGCAGTAA AGCCCCCCCC TGAGCCCTCC
    TATGATTTCT CTTCACCTGT CGACAGCTGC TCTTCTGACT TCACCTCGGG CTTGAGTGAT
    GGCAATGACG GCCAATCAGA GAGGAGCAGG ATGGAGGCAA CGATGAGGGA AGCAACCAAA
    CAGTTTAGAA GGAGAGCCAG AGCTCGTCTC CGAATCATAG GGGTGTCTGA CAAGGTGGAC
    CGAGTGGTGG AGTGTCAGCT GCAGACTCAC AACAACAAGA TGGTCACGTT TAAGTTTGAC
    CTGGATGGAG ACAATCCCGA GGACATCGCA TCTGTGATGA TTCACAGAGA TTTTATCTTA
    CCGTCTGAGC GAGAAGGATT TATCCTCCGC ATGTATGACA TCATCCAGAG GGCGGAGTCT
    ATGATACACC AGCATCATCC TGCAGTCTTG TACCAGCCTT CTCAGCTGAT GACCTCTGCT
    CCCCAGTCAA ACTGCCTCTG CTTTCAACCT TTTCAGCCAA AGTCAGCAGT GACTAACCTG
    TCCAGGACTT TGTCTTCCTC CTCCTTACCT GACATAGATG AAAGTGACAC CACCCCCTCA
    AAGGCAATTG ACTTCAGCAT TGACCCTGAT GCTGTTCCCC CCGTGAGGCC GTTGAGGTCA
    CAGTCCTTCC AGACGTCATC AGCCTCTTCC TATGAGCCCC CCCTTTACCC TTACCATTAC
    CCCCATCCTG AATCTACCAC TCCCCCCCTC TCTCAGAATC TGCTTCCTTC TCATCTTTAC
    TCCACCTATA CAGCCTCTTT CTCCACGCCC AGCTCCGCCC CCCAGATTCC ACGAGTCCAC
    AGCAACCCCT CCCTCTTTAC CTCCACACCA TCATCTTTAG CCCCTCCCTC TCCTAGGTGG
    CCACCCCCTG ACCAGCCTCT CTTCTCTTTG GCCAATGTAC TGTCTGTGGC CATGAGTGTG
    GCCCACAGCC TCATGCCCCC ACCCGGACAT CCGAGTCAAG GCTTTCAACA CCAGGCTCCT
    CCCCCGCCTT TCCCCCAGTC CACGTTCTCA CCCCCCATGT CTCCATCTCT AAAACGGAAC
    CTTCAGACTT TGACAAACAC CTCCACCGCT GAACCCCACC AACCCCCCCA GGCCTATCAC
    ACAGGCTTGG AGCCAGAAGT CCAAAAGACA GTGATGGACG GCGGAGCTGC AGATCAGTGT
    CCCGGTCCTG CCCGACCACG GCTCGGCTCG GCTCCCTCCT CCCAGGGTTC AGAGACCTCC
    GTTCTTCCCC GTCAGGCCAG CCTCTCAAAT CCCAGAGGAG AGTTTTCAGA TTCCTCCACC
    TCGTCCTCAG CCTCCCCTCC TCCCCCATCG GGTTTCTACC CCAAAGTGTC CCCCCCAGTG
    TCCCCATCAC GCTCCATCTC TCCTACGTTG AAAGCGGGGA AAACAGCCTT CACTGTGGGC
    CGCTTCCAGG TGACCCCATC AACACCCGTT TCTGCTGTCA GTCATCATCA GCCACGCCCC
    TTTAGTGATG CTTCACCCAT CGCTCACTCC CCCCCTCCTA CTCAGAAGAA CCAATCAGAG
    AGCAGCTCAA GAAGCAGTTC AGAAGAAGAC AGCCAATCAG AAATCAGCAA CCACACAGTC
    ATCAATACAA CATATGGTTA CCGTGACAAC CCCACCAGTC AAGACGAGAA GTGCATGAGC
    AGTCTTAAAC TGGGAATGAC TATGTGGGAG GGGCCAGCCA ACAGCCAATC ACGTAGCCGT
    TCTGCTACTT ACAGCTCTGA CGAATCAGAG GGTGAGGAGA TGTGGGAGGA GCTACTGGAG
    CTTCGTAAAA GACACTTGGT GGAGGTCCAG AATCTACAGA TGAGTCAAAA ACAAGAAATG
    GAGGAGTTGT ACAGACGGAA GGGTAAAGCC CTACCACCTG GGATCGTCCC TCCAGCCGCC
    ATGCTGAACC ACCGCCAACG CCGCCTCTCC AAGACAGGAA ACTATCCTCT GGCACGCCGA
    AGCAGCCTCC AGAGACTAGA TCTGCCGCCC CCTGCAGGGA TCATGAGGAA GGGCTCGGTC
    AGCGGTAGCA GTAGTGGATC TCAAGACCGG ATCCTGAAAG GAGTGACGTT TGCTCCTGGT
    CACAGCTGCA TGGTGAGCAA CAGAGGACCA GAACCAGCTC CATACCCGGA TCTCTGTAGA
    AGTCAGGACC AGAATCACAG TCAGGACCAG AATCACAGTC AGGACCAGGA CTACGACCAA
    ATTCACAGTC AGGACGAGGT TCAGGTCCAG GTCCAGGACC AGGGGCCTCA TTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023812929.1
    CDS917..5533
    Translation

    Target ORF information:

    RefSeq Version XM_023957161.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes serine/threonine-protein kinase WNK1 (LOC101162572), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023957161.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGCACT CCGCTGAAGA TGAAGAAGAG GAGAACTCTG CTCTTCATCT CAAGGACTCC 
    GACCAATCAG AGGCTTCCGA TCCACACACA GGTCATGATG TCAGCTCAGA CTCTGCCGAT
    GAAATGGGCA TCAATCTCTC AGAATTAAAC AGTGAGATAT TACCATGGCA ACGCACCCAG
    GAGGAGGAGC ACGACGAAGA GGAAATGAAC GCTGTGGCGT CGTCGCCTGA TGGGCGGTTC
    CTGAAGTTCA ACATCGAGAT CGGGAGGGGC TCCTTCAAAA CCGTTTACAA AGGGCTGGAC
    ACGGAGACCA CAGTGGAGGT GGCCTGGTGC GAGCTCCAGA CCCACCGGCT GAGCAGGGCC
    GAGCGTCAGC GTTTCAGCGA GGAGGTGGAG ATGCTCAAAG CGCTTCAGCA CCCCAACATC
    GTCCGTTTCT TCGATTCCTG GAAGTCCGCG CTGAAGGGCC ACAAATGCAC CATCCTGGTG
    ACGGAGCTCA TGACGTCTGG AACGCTGAAG ATGTACCTGC GCAGGTTCCG TCAGATGAAG
    CTGAAGCTGC TGCAGCGCTG GAGCTTCCAG GTCCTGCGGG GGCTTCACTT CCTGCACTCA
    CGTTCCCCCC CCATCATACA CCGTGACCTC AAATGTGACA ACATCTTCAT CACGGGTCCC
    TCCGCCTCCG TCAAAATCGG AGACCTGGGC CTGGCAACGC TGAAGAAGGC CTCGTTTGCT
    AAGAGCGTGA TCGGAACGCC GGAGTTCATG GCTCCAGAGA TGTACGAGGA GAAGTACGAC
    GAGGCTGTGG ACGTGTACGC CTTCGGCATG TGCATGCTGG AGATGGCTAC GTCCGAGTAT
    CCGTACCACG AGTGTCACAG CGCTGCCCAG ATCTACCGCA AGGTCACCAA TGGAATCAAA
    CCGGACAGTT TCTTCAAAGT GGAAGTTCCA GAACTGAAAG AAATCATTGA AGGCTGCATT
    CGGACCAGGA GCAGTGAAAG GTTTACAGTC CAGGACCTCT TGGAGCACAG CTTCTTTCAG
    GAGAACACCG GCGTCCATGT GGAGCTGGCA GAGGAAGACG ACGGCTCCAA GGCGGCGCTG
    AAGCTGTGGC TCCGGATGGA CGACAAGGAG AAGCTCCGGG GGAAGTATAA AGACAACGAC
    GCCATCGAGT TCCTGTACGA ACTCTACAAG GATGTGCCAG AGGAGGTCGC CCAGGAGATG
    GTCATTTTGG GATTTGTGTT CAAAGCAGAC TACAAGGTGA TTGCCAAAGC CATTCGGCAT
    CGAGTGACAG CCATAAAGTA TCAGCGGGAA AAACGGCGCC TCCTGCTGGA GGAAGGAGTG
    AACAGTCAGA AGCAAACTGA CATCGAGGAA GAGCTTCATC TTCATCAACG AGCTGAGCTG
    TCCAATCAGA CGCTCCCAGT TTCAACAGGA GCCAGAGGAG CGGCCAGTCA GGGGGACTCT
    GCTGCATCCA CACAAGCTCC GCCCCTGACT GCAACAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCTCT GACTGCAACA GCACAAGCTC CGCCCCTGAC TGCATCAGCA
    CAAGCTCCGC CCCTGACTGC AACAGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCAGC ACAAGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGACTGCAT CCACACACGC TCCTTCTCTA ACTGCATCGG CACAAGCTCC GCCCCTGACT
    GCATCAGCAC AAGCTCCGCC CCTGACTGCA TCCACACACG CTCCGCCCCT GACTGCATCA
    GCACAAGCTC CGCCCCTGAT TGCATCCACA CACTCTCCTT CCCTAACTGC ATCGGCACAA
    GCTCCACCCC TGACTGCATC CACACACGCT CCTTCCCTAA CTGCATCGGC ACAAGCTCCG
    CCCCTGACTG CATCGGCACA AACTCCGCCC CTGACTGCAT CGGCACAAGC TCCGCCCCTG
    ACTGCATCAG CACAAGCTCC GCCCCTGACT GCATCCACAC ACGCTCCGCC CCTGACTTTA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CAAGCTCCGC CCAGCAGCCG GGGGGATTCC GGGATCAACA CTGTCTCCAG CAGAGCTGAT
    GGCGACACCG AGGAGGACGG TGCGATCCGA CTAGTCTCCC ACCCCCCCAC TGCCTACCAT
    GTGATTGAAA CCACGCCTCT TCTTGTTCCT AACCCCACTC CTCCATCAGT ACCTCCACAG
    ACGGAGGTTC CTTCTGTTTT GACCCGCACA GCTTCCAAAG CTCCGCCCTT TCCCATACTA
    CGCTTTCCTA AAAGCATTGC TGTGTCCCAG GCTGCAGTAA AGCCCCCCCC TGAGCCCTCC
    TATGATTTCT CTTCACCTGT CGACAGCTGC TCTTCTGACT TCACCTCGGG CTTGAGTGAT
    GGCAATGACG GCCAATCAGA GAGGAGCAGG ATGGAGGCAA CGATGAGGGA AGCAACCAAA
    CAGTTTAGAA GGAGAGCCAG AGCTCGTCTC CGAATCATAG GGGTGTCTGA CAAGGTGGAC
    CGAGTGGTGG AGTGTCAGCT GCAGACTCAC AACAACAAGA TGGTCACGTT TAAGTTTGAC
    CTGGATGGAG ACAATCCCGA GGACATCGCA TCTGTGATGA TTCACAGAGA TTTTATCTTA
    CCGTCTGAGC GAGAAGGATT TATCCTCCGC ATGTATGACA TCATCCAGAG GGCGGAGTCT
    ATGATACACC AGCATCATCC TGCAGTCTTG TACCAGCCTT CTCAGCTGAT GACCTCTGCT
    CCCCAGTCAA ACTGCCTCTG CTTTCAACCT TTTCAGCCAA AGTCAGCAGT GACTAACCTG
    TCCAGGACTT TGTCTTCCTC CTCCTTACCT GACATAGATG AAAGTGACAC CACCCCCTCA
    AAGGCAATTG ACTTCAGCAT TGACCCTGAT GCTGTTCCCC CCGTGAGGCC GTTGAGGTCA
    CAGTCCTTCC AGACGTCATC AGCCTCTTCC TATGAGCCCC CCCTTTACCC TTACCATTAC
    CCCCATCCTG AATCTACCAC TCCCCCCCTC TCTCAGAATC TGCTTCCTTC TCATCTTTAC
    TCCACCTATA CAGCCTCTTT CTCCACGCCC AGCTCCGCCC CCCAGATTCC ACGAGTCCAC
    AGCAACCCCT CCCTCTTTAC CTCCACACCA TCATCTTTAG CCCCTCCCTC TCCTAGGTGG
    CCACCCCCTG ACCAGCCTCT CTTCTCTTTG GCCAATGTAC TGTCTGTGGC CATGAGTGTG
    GCCCACAGCC TCATGCCCCC ACCCGGACAT CCGAGTCAAG GCTTTCAACA CCAGGCTCCT
    CCCCCGCCTT TCCCCCAGTC CACGTTCTCA CCCCCCATGT CTCCATCTCT AAAACGGAAC
    CTTCAGACTT TGACAAACAC CTCCACCGCT GAACCCCACC AACCCCCCCA GGCCTATCAC
    ACAGGCTTGG AGCCAGAAGT CCAAAAGACA GTGATGGACG GCGGAGCTGC AGATCAGTGT
    CCCGGTCCTG CCCGACCACG GCTCGGCTCG GCTCCCTCCT CCCAGGGTTC AGAGACCTCC
    GTTCTTCCCC GTCAGGCCAG CCTCTCAAAT CCCAGAGGAG AGTTTTCAGA TTCCTCCACC
    TCGTCCTCAG CCTCCCCTCC TCCCCCATCG GGTTTCTACC CCAAAGTGTC CCCCCCAGTG
    TCCCCATCAC GCTCCATCTC TCCTACGTTG AAAGCGGGGA AAACAGCCTT CACTGTGGGC
    CGCTTCCAGG TGACCCCATC AACACCCGTT TCTGCTGTCA GTCATCATCA GCCACGCCCC
    TTTAGTGATG CTTCACCCAT CGCTCACTCC CCCCCTCCTA CTCAGAAGAA CCAATCAGAG
    AGCAGCTCAA GAAGCAGTTC AGAAGAAGAC AGCCAATCAG AAATCAGCAA CCACACAGTC
    ATCAATACAA CATATGGTTA CCGTGACAAC CCCACCAGTC AAGACGAGAA GTGCATGAGC
    AGTCTTAAAC TGGGAATGAC TATGTGGGAG GGGCCAGCCA ACAGCCAATC ACGTAGCCGT
    TCTGCTACTT ACAGCTCTGA CGAATCAGAG GGTGAGGAGA TGTGGGAGGA GCTACTGGAG
    CTTCGTAAAA GACACTTGGT GGAGGTCCAG AATCTACAGA TGAGTCAAAA ACAAGAAATG
    GAGGAGTTGT ACAGACGGAA GGGTAAAGCC CTACCACCTG GGATCGTCCC TCCAGCCGCC
    ATGCTGAACC ACCGCCAACG CCGCCTCTCC AAGACAGGAA ACTATCCTCT GGCACGCCGA
    AGCAGCCTCC AGAGACTAGA TCTGCCGCCC CCTGCAGGGA TCATGAGGAA GGGCTCGGTC
    AGCGGTAGCA GTAGTGGATC TCAAGACCGG ATCCTGAAAG GAGTGACGTT TGCTCCTGGT
    CACAGCTGCA TGGTGAGCAA CAGAGGACCA GAACCAGCTC CATACCCGGA TCTCTGTAGA
    AGTCAGGACC AGAATCACAG TCAGGACCAG AATCACAGTC AGGACCAGGA CTACGACCAA
    ATTCACAGTC AGGACGAGGT TCAGGTCCAG GTCCAGGACC AGGGGCCTCA TTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa117515
    Clone ID Related Accession (Same CDS sequence) XM_023957162.1
    Accession Version XM_023957162.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1518537600000
    Organism Oryzias latipes(Japanese medaka)
    Product serine/threonine-protein kinase WNK1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019866.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGCACT CCGCTGAAGA TGAAGAAGAG GAGAACTCTG CTCTTCATCT CAAGGACTCC 
    GACCAATCAG AGGCTTCCGA TCCACACACA GGTCATGATG TCAGCTCAGA CTCTGCCGAT
    GAAATGGGCA TCAATCTCTC AGAATTAAAC AGTGAGATAT TACCATGGCA ACGCACCCAG
    GAGGAGGAGC ACGACGAAGA GGAAATGAAC GCTGTGGCGT CGTCGCCTGA TGGGCGGTTC
    CTGAAGTTCA ACATCGAGAT CGGGAGGGGC TCCTTCAAAA CCGTTTACAA AGGGCTGGAC
    ACGGAGACCA CAGTGGAGGT GGCCTGGTGC GAGCTCCAGA CCCACCGGCT GAGCAGGGCC
    GAGCGTCAGC GTTTCAGCGA GGAGGTGGAG ATGCTCAAAG CGCTTCAGCA CCCCAACATC
    GTCCGTTTCT TCGATTCCTG GAAGTCCGCG CTGAAGGGCC ACAAATGCAC CATCCTGGTG
    ACGGAGCTCA TGACGTCTGG AACGCTGAAG ATGTACCTGC GCAGGTTCCG TCAGATGAAG
    CTGAAGCTGC TGCAGCGCTG GAGCTTCCAG GTCCTGCGGG GGCTTCACTT CCTGCACTCA
    CGTTCCCCCC CCATCATACA CCGTGACCTC AAATGTGACA ACATCTTCAT CACGGGTCCC
    TCCGCCTCCG TCAAAATCGG AGACCTGGGC CTGGCAACGC TGAAGAAGGC CTCGTTTGCT
    AAGAGCGTGA TCGGAACGCC GGAGTTCATG GCTCCAGAGA TGTACGAGGA GAAGTACGAC
    GAGGCTGTGG ACGTGTACGC CTTCGGCATG TGCATGCTGG AGATGGCTAC GTCCGAGTAT
    CCGTACCACG AGTGTCACAG CGCTGCCCAG ATCTACCGCA AGGTCACCAA TGGAATCAAA
    CCGGACAGTT TCTTCAAAGT GGAAGTTCCA GAACTGAAAG AAATCATTGA AGGCTGCATT
    CGGACCAGGA GCAGTGAAAG GTTTACAGTC CAGGACCTCT TGGAGCACAG CTTCTTTCAG
    GAGAACACCG GCGTCCATGT GGAGCTGGCA GAGGAAGACG ACGGCTCCAA GGCGGCGCTG
    AAGCTGTGGC TCCGGATGGA CGACAAGGAG AAGCTCCGGG GGAAGTATAA AGACAACGAC
    GCCATCGAGT TCCTGTACGA ACTCTACAAG GATGTGCCAG AGGAGGTCGC CCAGGAGATG
    GTCATTTTGG GATTTGTGTT CAAAGCAGAC TACAAGGTGA TTGCCAAAGC CATTCGGCAT
    CGAGTGACAG CCATAAAGTA TCAGCGGGAA AAACGGCGCC TCCTGCTGGA GGAAGGAGTG
    AACAGTCAGA AGCAAACTGA CATCGAGGAA GAGCTTCATC TTCATCAACG AGCTGAGCTG
    TCCAATCAGA CGCTCCCAGT TTCAACAGGA GCCAGAGGAG CGGCCAGTCA GGGGGACTCT
    GCTGCATCCA CACAAGCTCC GCCCCTGACT GCAACAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCTCT GACTGCAACA GCACAAGCTC CGCCCCTGAC TGCATCAGCA
    CAAGCTCCGC CCCTGACTGC AACAGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCAGC ACAAGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGACTGCAT CCACACACGC TCCTTCTCTA ACTGCATCGG CACAAGCTCC GCCCCTGACT
    GCATCAGCAC AAGCTCCGCC CCTGACTGCA TCCACACACG CTCCGCCCCT GACTGCATCA
    GCACAAGCTC CGCCCCTGAT TGCATCCACA CACTCTCCTT CCCTAACTGC ATCGGCACAA
    GCTCCACCCC TGACTGCATC CACACACGCT CCTTCCCTAA CTGCATCGGC ACAAGCTCCG
    CCCCTGACTG CATCGGCACA AACTCCGCCC CTGACTGCAT CGGCACAAGC TCCGCCCCTG
    ACTGCATCAG CACAAGCTCC GCCCCTGACT GCATCCACAC ACGCTCCGCC CCTGACTTTA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CAAGCTCCGC CCAGCAGCCG GGGGGATTCC GGGATCAACA CTGTCTCCAG CAGAGCTGAT
    GGCGACACCG AGGAGGACGG TGCGATCCGA CTAGTCTCCC ACCCCCCCAC TGCCTCAGAC
    CATGTGATTG AAACCACGCC TCTTCTTGTT CCTAACCCCA CTCCTCCATC AGTACCTCCA
    CAGACGGAGG TTCCTTCTGT TTTGACCCGC ACAGCTTCCA AAGCTCCGCC CTTTCCCATA
    CTACGCTTTC CTAAAAGCAT TGCTGTGTCC CAGGCTGCAG TAAAGCCCCC CCCTGAGCCC
    TCCTATGATT TCTCTTCACC TGTCGACAGC TGCTCTTCTG ACTTCACCTC GGGCTTGAGT
    GATGGCAATG ACGGCCAATC AGAGAGGAGC AGGATGGAGG CAACGATGAG GGAAGCAACC
    AAACAGTTTA GAAGGAGAGC CAGAGCTCGT CTCCGAATCA TAGGGGTGTC TGACAAGGTG
    GACCGAGTGG TGGAGTGTCA GCTGCAGACT CACAACAACA AGATGGTCAC GTTTAAGTTT
    GACCTGGATG GAGACAATCC CGAGGACATC GCATCTGTGA TGATTCACAG AGATTTTATC
    TTACCGTCTG AGCGAGAAGG ATTTATCCTC CGCATGTATG ACATCATCCA GAGGGCGGAG
    TCTATGATAC ACCAGCATCA TCCTGCAGTC TTGTACCAGC CTTCTCAGCT GATGACCTCT
    GCTCCCCAGC CAAAGTCAGC AGTGACTAAC CTGTCCAGGA CTTTGTCTTC CTCCTCCTTA
    CCTGACATAG ATGAAAGTGA CACCACCCCC TCAAAGGCAA TTGACTTCAG CATTGACCCT
    GATGCTGTTC CCCCCGTGAG GCCGTTGAGG TCACAGTCCT TCCAGACGTC ATCAGCCTCT
    TCCTATGAGC CCCCCCTTTA CCCTTACCAT TACCCCCATC CTGAATCTAC CACTCCCCCC
    CTCTCTCAGA ATCTGCTTCC TTCTCATCTT TACTCCACCT ATACAGCCTC TTTCTCCACG
    CCCAGCTCCG CCCCCCAGAT TCCACGAGTC CACAGCAACC CCTCCCTCTT TACCTCCACA
    CCATCATCTT TAGCCCCTCC CTCTCCTAGG TGGCCACCCC CTGACCAGCC TCTCTTCTCT
    TTGGCCAATG TACTGTCTGT GGCCATGAGT GTGGCCCACA GCCTCATGCC CCCACCCGGA
    CATCCGAGTC AAGGCTTTCA ACACCAGGCT CCTCCCCCGC CTTTCCCCCA GTCCACGTTC
    TCACCCCCCA TGTCTCCATC TCTAAAACGG AACCTTCAGA CTTTGACAAA CACCTCCACC
    GCTGAACCCC ACCAACCCCC CCAGGCCTAT CACACAGGCT TGGAGCCAGA AGTCCAAAAG
    ACAGTGATGG ACGGCGGAGC TGCAGATCAG TGTCCCGGTC CTGCCCGACC ACGGCTCGGC
    TCGGCTCCCT CCTCCCAGGG TTCAGAGACC TCCGTTCTTC CCCGTCAGGC CAGCCTCTCA
    AATCCCAGAG GAGAGTTTTC AGATTCCTCC ACCTCGTCCT CAGCCTCCCC TCCTCCCCCA
    TCGGGTTTCT ACCCCAAAGT GTCCCCCCCA GTGTCCCCAT CACGCTCCAT CTCTCCTACG
    TTGAAAGCGG GGAAAACAGC CTTCACTGTG GGCCGCTTCC AGGTGACCCC ATCAACACCC
    GTTTCTGCTG TCAGTCATCA TCAGCCACGC CCCTTTAGTG ATGCTTCACC CATCGCTCAC
    TCCCCCCCTC CTACTCAGAA GAACCAATCA GAGAGCAGCT CAAGAAGCAG TTCAGAAGAA
    GACAGCCAAT CAGAAATCAG CAACCACACA GTCATCAATA CAACATATGG TTACCGTGAC
    AACCCCACCA GTCAAGACGA GAAGTGCATG AGCAGTCTTA AACTGGGAAT GACTATGTGG
    GAGGGGCCAG CCAACAGCCA ATCACGTAGC CGTTCTGCTA CTTACAGCTC TGACGAATCA
    GAGGGTGAGG AGATGTGGGA GGAGCTACTG GAGCTTCGTA AAAGACACTT GGTGGAGGTC
    CAGAATCTAC AGATGAGTCA AAAACAAGAA ATGGAGGAGT TGTACAGACG GAAGGGTAAA
    GCCCTACCAC CTGGGATCGT CCCTCCAGCC GCCATGCTGA ACCACCGCCA ACGCCGCCTC
    TCCAAGACAG GAAACTATCC TCTGGCACGC CGAAGCAGCC TCCAGAGACT AGATCTGCCG
    CCCCCTGCAG GGATCATGAG GAAGGGCTCG GTCAGCGGTA GCAGTAGTGG ATCTCAAGAC
    CGGATCCTGA AAGGAGTGAC GTTTGCTCCT GGTCACAGCT GCATGGTGAG CAACAGAGGA
    CCAGAACCAG CTCCATACCC GGATCTCTGT AGAAGTCAGG ACCAGAATCA CAGTCAGGAC
    CAGAATCACA GTCAGGACCA GGACTACGAC CAAATTCACA GTCAGGACGA GGTTCAGGTC
    CAGGTCCAGG ACCAGGGGCC TCATTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023812930.1
    CDS912..5501
    Translation

    Target ORF information:

    RefSeq Version XM_023957162.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes serine/threonine-protein kinase WNK1 (LOC101162572), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023957162.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGCACT CCGCTGAAGA TGAAGAAGAG GAGAACTCTG CTCTTCATCT CAAGGACTCC 
    GACCAATCAG AGGCTTCCGA TCCACACACA GGTCATGATG TCAGCTCAGA CTCTGCCGAT
    GAAATGGGCA TCAATCTCTC AGAATTAAAC AGTGAGATAT TACCATGGCA ACGCACCCAG
    GAGGAGGAGC ACGACGAAGA GGAAATGAAC GCTGTGGCGT CGTCGCCTGA TGGGCGGTTC
    CTGAAGTTCA ACATCGAGAT CGGGAGGGGC TCCTTCAAAA CCGTTTACAA AGGGCTGGAC
    ACGGAGACCA CAGTGGAGGT GGCCTGGTGC GAGCTCCAGA CCCACCGGCT GAGCAGGGCC
    GAGCGTCAGC GTTTCAGCGA GGAGGTGGAG ATGCTCAAAG CGCTTCAGCA CCCCAACATC
    GTCCGTTTCT TCGATTCCTG GAAGTCCGCG CTGAAGGGCC ACAAATGCAC CATCCTGGTG
    ACGGAGCTCA TGACGTCTGG AACGCTGAAG ATGTACCTGC GCAGGTTCCG TCAGATGAAG
    CTGAAGCTGC TGCAGCGCTG GAGCTTCCAG GTCCTGCGGG GGCTTCACTT CCTGCACTCA
    CGTTCCCCCC CCATCATACA CCGTGACCTC AAATGTGACA ACATCTTCAT CACGGGTCCC
    TCCGCCTCCG TCAAAATCGG AGACCTGGGC CTGGCAACGC TGAAGAAGGC CTCGTTTGCT
    AAGAGCGTGA TCGGAACGCC GGAGTTCATG GCTCCAGAGA TGTACGAGGA GAAGTACGAC
    GAGGCTGTGG ACGTGTACGC CTTCGGCATG TGCATGCTGG AGATGGCTAC GTCCGAGTAT
    CCGTACCACG AGTGTCACAG CGCTGCCCAG ATCTACCGCA AGGTCACCAA TGGAATCAAA
    CCGGACAGTT TCTTCAAAGT GGAAGTTCCA GAACTGAAAG AAATCATTGA AGGCTGCATT
    CGGACCAGGA GCAGTGAAAG GTTTACAGTC CAGGACCTCT TGGAGCACAG CTTCTTTCAG
    GAGAACACCG GCGTCCATGT GGAGCTGGCA GAGGAAGACG ACGGCTCCAA GGCGGCGCTG
    AAGCTGTGGC TCCGGATGGA CGACAAGGAG AAGCTCCGGG GGAAGTATAA AGACAACGAC
    GCCATCGAGT TCCTGTACGA ACTCTACAAG GATGTGCCAG AGGAGGTCGC CCAGGAGATG
    GTCATTTTGG GATTTGTGTT CAAAGCAGAC TACAAGGTGA TTGCCAAAGC CATTCGGCAT
    CGAGTGACAG CCATAAAGTA TCAGCGGGAA AAACGGCGCC TCCTGCTGGA GGAAGGAGTG
    AACAGTCAGA AGCAAACTGA CATCGAGGAA GAGCTTCATC TTCATCAACG AGCTGAGCTG
    TCCAATCAGA CGCTCCCAGT TTCAACAGGA GCCAGAGGAG CGGCCAGTCA GGGGGACTCT
    GCTGCATCCA CACAAGCTCC GCCCCTGACT GCAACAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCTCT GACTGCAACA GCACAAGCTC CGCCCCTGAC TGCATCAGCA
    CAAGCTCCGC CCCTGACTGC AACAGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCAGC ACAAGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGACTGCAT CCACACACGC TCCTTCTCTA ACTGCATCGG CACAAGCTCC GCCCCTGACT
    GCATCAGCAC AAGCTCCGCC CCTGACTGCA TCCACACACG CTCCGCCCCT GACTGCATCA
    GCACAAGCTC CGCCCCTGAT TGCATCCACA CACTCTCCTT CCCTAACTGC ATCGGCACAA
    GCTCCACCCC TGACTGCATC CACACACGCT CCTTCCCTAA CTGCATCGGC ACAAGCTCCG
    CCCCTGACTG CATCGGCACA AACTCCGCCC CTGACTGCAT CGGCACAAGC TCCGCCCCTG
    ACTGCATCAG CACAAGCTCC GCCCCTGACT GCATCCACAC ACGCTCCGCC CCTGACTTTA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CAAGCTCCGC CCAGCAGCCG GGGGGATTCC GGGATCAACA CTGTCTCCAG CAGAGCTGAT
    GGCGACACCG AGGAGGACGG TGCGATCCGA CTAGTCTCCC ACCCCCCCAC TGCCTCAGAC
    CATGTGATTG AAACCACGCC TCTTCTTGTT CCTAACCCCA CTCCTCCATC AGTACCTCCA
    CAGACGGAGG TTCCTTCTGT TTTGACCCGC ACAGCTTCCA AAGCTCCGCC CTTTCCCATA
    CTACGCTTTC CTAAAAGCAT TGCTGTGTCC CAGGCTGCAG TAAAGCCCCC CCCTGAGCCC
    TCCTATGATT TCTCTTCACC TGTCGACAGC TGCTCTTCTG ACTTCACCTC GGGCTTGAGT
    GATGGCAATG ACGGCCAATC AGAGAGGAGC AGGATGGAGG CAACGATGAG GGAAGCAACC
    AAACAGTTTA GAAGGAGAGC CAGAGCTCGT CTCCGAATCA TAGGGGTGTC TGACAAGGTG
    GACCGAGTGG TGGAGTGTCA GCTGCAGACT CACAACAACA AGATGGTCAC GTTTAAGTTT
    GACCTGGATG GAGACAATCC CGAGGACATC GCATCTGTGA TGATTCACAG AGATTTTATC
    TTACCGTCTG AGCGAGAAGG ATTTATCCTC CGCATGTATG ACATCATCCA GAGGGCGGAG
    TCTATGATAC ACCAGCATCA TCCTGCAGTC TTGTACCAGC CTTCTCAGCT GATGACCTCT
    GCTCCCCAGC CAAAGTCAGC AGTGACTAAC CTGTCCAGGA CTTTGTCTTC CTCCTCCTTA
    CCTGACATAG ATGAAAGTGA CACCACCCCC TCAAAGGCAA TTGACTTCAG CATTGACCCT
    GATGCTGTTC CCCCCGTGAG GCCGTTGAGG TCACAGTCCT TCCAGACGTC ATCAGCCTCT
    TCCTATGAGC CCCCCCTTTA CCCTTACCAT TACCCCCATC CTGAATCTAC CACTCCCCCC
    CTCTCTCAGA ATCTGCTTCC TTCTCATCTT TACTCCACCT ATACAGCCTC TTTCTCCACG
    CCCAGCTCCG CCCCCCAGAT TCCACGAGTC CACAGCAACC CCTCCCTCTT TACCTCCACA
    CCATCATCTT TAGCCCCTCC CTCTCCTAGG TGGCCACCCC CTGACCAGCC TCTCTTCTCT
    TTGGCCAATG TACTGTCTGT GGCCATGAGT GTGGCCCACA GCCTCATGCC CCCACCCGGA
    CATCCGAGTC AAGGCTTTCA ACACCAGGCT CCTCCCCCGC CTTTCCCCCA GTCCACGTTC
    TCACCCCCCA TGTCTCCATC TCTAAAACGG AACCTTCAGA CTTTGACAAA CACCTCCACC
    GCTGAACCCC ACCAACCCCC CCAGGCCTAT CACACAGGCT TGGAGCCAGA AGTCCAAAAG
    ACAGTGATGG ACGGCGGAGC TGCAGATCAG TGTCCCGGTC CTGCCCGACC ACGGCTCGGC
    TCGGCTCCCT CCTCCCAGGG TTCAGAGACC TCCGTTCTTC CCCGTCAGGC CAGCCTCTCA
    AATCCCAGAG GAGAGTTTTC AGATTCCTCC ACCTCGTCCT CAGCCTCCCC TCCTCCCCCA
    TCGGGTTTCT ACCCCAAAGT GTCCCCCCCA GTGTCCCCAT CACGCTCCAT CTCTCCTACG
    TTGAAAGCGG GGAAAACAGC CTTCACTGTG GGCCGCTTCC AGGTGACCCC ATCAACACCC
    GTTTCTGCTG TCAGTCATCA TCAGCCACGC CCCTTTAGTG ATGCTTCACC CATCGCTCAC
    TCCCCCCCTC CTACTCAGAA GAACCAATCA GAGAGCAGCT CAAGAAGCAG TTCAGAAGAA
    GACAGCCAAT CAGAAATCAG CAACCACACA GTCATCAATA CAACATATGG TTACCGTGAC
    AACCCCACCA GTCAAGACGA GAAGTGCATG AGCAGTCTTA AACTGGGAAT GACTATGTGG
    GAGGGGCCAG CCAACAGCCA ATCACGTAGC CGTTCTGCTA CTTACAGCTC TGACGAATCA
    GAGGGTGAGG AGATGTGGGA GGAGCTACTG GAGCTTCGTA AAAGACACTT GGTGGAGGTC
    CAGAATCTAC AGATGAGTCA AAAACAAGAA ATGGAGGAGT TGTACAGACG GAAGGGTAAA
    GCCCTACCAC CTGGGATCGT CCCTCCAGCC GCCATGCTGA ACCACCGCCA ACGCCGCCTC
    TCCAAGACAG GAAACTATCC TCTGGCACGC CGAAGCAGCC TCCAGAGACT AGATCTGCCG
    CCCCCTGCAG GGATCATGAG GAAGGGCTCG GTCAGCGGTA GCAGTAGTGG ATCTCAAGAC
    CGGATCCTGA AAGGAGTGAC GTTTGCTCCT GGTCACAGCT GCATGGTGAG CAACAGAGGA
    CCAGAACCAG CTCCATACCC GGATCTCTGT AGAAGTCAGG ACCAGAATCA CAGTCAGGAC
    CAGAATCACA GTCAGGACCA GGACTACGAC CAAATTCACA GTCAGGACGA GGTTCAGGTC
    CAGGTCCAGG ACCAGGGGCC TCATTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa117516
    Clone ID Related Accession (Same CDS sequence) XM_023957163.1
    Accession Version XM_023957163.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4584bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1518537600000
    Organism Oryzias latipes(Japanese medaka)
    Product serine/threonine-protein kinase WNK1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019866.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGCACT CCGCTGAAGA TGAAGAAGAG GAGAACTCTG CTCTTCATCT CAAGGACTCC 
    GACCAATCAG AGGCTTCCGA TCCACACACA GGTCATGATG TCAGCTCAGA CTCTGCCGAT
    GAAATGGGCA TCAATCTCTC AGAATTAAAC AGTGAGATAT TACCATGGCA ACGCACCCAG
    GAGGAGGAGC ACGACGAAGA GGAAATGAAC GCTGTGGCGT CGTCGCCTGA TGGGCGGTTC
    CTGAAGTTCA ACATCGAGAT CGGGAGGGGC TCCTTCAAAA CCGTTTACAA AGGGCTGGAC
    ACGGAGACCA CAGTGGAGGT GGCCTGGTGC GAGCTCCAGA CCCACCGGCT GAGCAGGGCC
    GAGCGTCAGC GTTTCAGCGA GGAGGTGGAG ATGCTCAAAG CGCTTCAGCA CCCCAACATC
    GTCCGTTTCT TCGATTCCTG GAAGTCCGCG CTGAAGGGCC ACAAATGCAC CATCCTGGTG
    ACGGAGCTCA TGACGTCTGG AACGCTGAAG ATGTACCTGC GCAGGTTCCG TCAGATGAAG
    CTGAAGCTGC TGCAGCGCTG GAGCTTCCAG GTCCTGCGGG GGCTTCACTT CCTGCACTCA
    CGTTCCCCCC CCATCATACA CCGTGACCTC AAATGTGACA ACATCTTCAT CACGGGTCCC
    TCCGCCTCCG TCAAAATCGG AGACCTGGGC CTGGCAACGC TGAAGAAGGC CTCGTTTGCT
    AAGAGCGTGA TCGGAACGCC GGAGTTCATG GCTCCAGAGA TGTACGAGGA GAAGTACGAC
    GAGGCTGTGG ACGTGTACGC CTTCGGCATG TGCATGCTGG AGATGGCTAC GTCCGAGTAT
    CCGTACCACG AGTGTCACAG CGCTGCCCAG ATCTACCGCA AGGTCACCAA TGGAATCAAA
    CCGGACAGTT TCTTCAAAGT GGAAGTTCCA GAACTGAAAG AAATCATTGA AGGCTGCATT
    CGGACCAGGA GCAGTGAAAG GTTTACAGTC CAGGACCTCT TGGAGCACAG CTTCTTTCAG
    GAGAACACCG GCGTCCATGT GGAGCTGGCA GAGGAAGACG ACGGCTCCAA GGCGGCGCTG
    AAGCTGTGGC TCCGGATGGA CGACAAGGAG AAGCTCCGGG GGAAGTATAA AGACAACGAC
    GCCATCGAGT TCCTGTACGA ACTCTACAAG GATGTGCCAG AGGAGGTCGC CCAGGAGATG
    GTCATTTTGG GATTTGTGTT CAAAGCAGAC TACAAGGTGA TTGCCAAAGC CATTCGGCAT
    CGAGTGACAG CCATAAAGTA TCAGCGGGAA AAACGGCGCC TCCTGCTGGA GGAAGGAGTG
    AACAGTCAGA AGCAAACTGA CATCGAGGAA GAGCTTCATC TTCATCAACG AGCTGAGCTG
    TCCAATCAGA CGCTCCCAGT TTCAACAGGA GCCAGAGGAG CGGCCAGTCA GGGGGACTCT
    GCTGCATCCA CACAAGCTCC GCCCCTGACT GCAACAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCTCT GACTGCAACA GCACAAGCTC CGCCCCTGAC TGCATCAGCA
    CAAGCTCCGC CCCTGACTGC AACAGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCAGC ACAAGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGACTGCAT CCACACACGC TCCTTCTCTA ACTGCATCGG CACAAGCTCC GCCCCTGACT
    GCATCAGCAC AAGCTCCGCC CCTGACTGCA TCCACACACG CTCCGCCCCT GACTGCATCA
    GCACAAGCTC CGCCCCTGAT TGCATCCACA CACTCTCCTT CCCTAACTGC ATCGGCACAA
    GCTCCACCCC TGACTGCATC CACACACGCT CCTTCCCTAA CTGCATCGGC ACAAGCTCCG
    CCCCTGACTG CATCGGCACA AACTCCGCCC CTGACTGCAT CGGCACAAGC TCCGCCCCTG
    ACTGCATCAG CACAAGCTCC GCCCCTGACT GCATCCACAC ACGCTCCGCC CCTGACTTTA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CAAGCTCCGC CCAGCAGCCG GGGGGATTCC GGGATCAACA CTGTCTCCAG CAGAGCTGAT
    GGCGACACCG AGGAGGACGC AGACCATGTG ATTGAAACCA CGCCTCTTCT TGTTCCTAAC
    CCCACTCCTC CATCAGTACC TCCACAGACG GAGGTTCCTT CTGTTTTGAC CCGCACAGCT
    TCCAAAGCTC CGCCCTTTCC CATACTACGC TTTCCTAAAA GCATTGCTGT GTCCCAGGCT
    GCAGTAAAGC CCCCCCCTGA GCCCTCCTAT GATTTCTCTT CACCTGTCGA CAGCTGCTCT
    TCTGACTTCA CCTCGGGCTT GAGTGATGGC AATGACGGCC AATCAGAGAG GAGCAGGATG
    GAGGCAACGA TGAGGGAAGC AACCAAACAG TTTAGAAGGA GAGCCAGAGC TCGTCTCCGA
    ATCATAGGGG TGTCTGACAA GGTGGACCGA GTGGTGGAGT GTCAGCTGCA GACTCACAAC
    AACAAGATGG TCACGTTTAA GTTTGACCTG GATGGAGACA ATCCCGAGGA CATCGCATCT
    GTGATGATTC ACAGAGATTT TATCTTACCG TCTGAGCGAG AAGGATTTAT CCTCCGCATG
    TATGACATCA TCCAGAGGGC GGAGTCTATG ATACACCAGC ATCATCCTGC AGTCTTGTAC
    CAGCCTTCTC AGCTGATGAC CTCTGCTCCC CAGTCAAACT GCCTCTGCTT TCAACCTTTT
    CAGCCAAAGT CAGCAGTGAC TAACCTGTCC AGGACTTTGT CTTCCTCCTC CTTACCTGAC
    ATAGATGAAA GTGACACCAC CCCCTCAAAG GCAATTGACT TCAGCATTGA CCCTGATGCT
    GTTCCCCCCG TGAGGCCGTT GAGGTCACAG TCCTTCCAGA CGTCATCAGC CTCTTCCTAT
    GAGCCCCCCC TTTACCCTTA CCATTACCCC CATCCTGAAT CTACCACTCC CCCCCTCTCT
    CAGAATCTGC TTCCTTCTCA TCTTTACTCC ACCTATACAG CCTCTTTCTC CACGCCCAGC
    TCCGCCCCCC AGATTCCACG AGTCCACAGC AACCCCTCCC TCTTTACCTC CACACCATCA
    TCTTTAGCCC CTCCCTCTCC TAGGTGGCCA CCCCCTGACC AGCCTCTCTT CTCTTTGGCC
    AATGTACTGT CTGTGGCCAT GAGTGTGGCC CACAGCCTCA TGCCCCCACC CGGACATCCG
    AGTCAAGGCT TTCAACACCA GGCTCCTCCC CCGCCTTTCC CCCAGTCCAC GTTCTCACCC
    CCCATGTCTC CATCTCTAAA ACGGAACCTT CAGACTTTGA CAAACACCTC CACCGCTGAA
    CCCCACCAAC CCCCCCAGGC CTATCACACA GGCTTGGAGC CAGAAGTCCA AAAGACAGTG
    ATGGACGGCG GAGCTGCAGA TCAGTGTCCC GGTCCTGCCC GACCACGGCT CGGCTCGGCT
    CCCTCCTCCC AGGGTTCAGA GACCTCCGTT CTTCCCCGTC AGGCCAGCCT CTCAAATCCC
    AGAGGAGAGT TTTCAGATTC CTCCACCTCG TCCTCAGCCT CCCCTCCTCC CCCATCGGGT
    TTCTACCCCA AAGTGTCCCC CCCAGTGTCC CCATCACGCT CCATCTCTCC TACGTTGAAA
    GCGGGGAAAA CAGCCTTCAC TGTGGGCCGC TTCCAGGTGA CCCCATCAAC ACCCGTTTCT
    GCTGTCAGTC ATCATCAGCC ACGCCCCTTT AGTGATGCTT CACCCATCGC TCACTCCCCC
    CCTCCTACTC AGAAGAACCA ATCAGAGAGC AGCTCAAGAA GCAGTTCAGA AGAAGACAGC
    CAATCAGAAA TCAGCAACCA CACAGTCATC AATACAACAT ATGGTTACCG TGACAACCCC
    ACCAGTCAAG ACGAGAAGTG CATGAGCAGT CTTAAACTGG GAATGACTAT GTGGGAGGGG
    CCAGCCAACA GCCAATCACG TAGCCGTTCT GCTACTTACA GCTCTGACGA ATCAGAGGGT
    GAGGAGATGT GGGAGGAGCT ACTGGAGCTT CGTAAAAGAC ACTTGGTGGA GGTCCAGAAT
    CTACAGATGA GTCAAAAACA AGAAATGGAG GAGTTGTACA GACGGAAGGG TAAAGCCCTA
    CCACCTGGGA TCGTCCCTCC AGCCGCCATG CTGAACCACC GCCAACGCCG CCTCTCCAAG
    ACAGGAAACT ATCCTCTGGC ACGCCGAAGC AGCCTCCAGA GACTAGATCT GCCGCCCCCT
    GCAGGGATCA TGAGGAAGGG CTCGGTCAGC GGTAGCAGTA GTGGATCTCA AGACCGGATC
    CTGAAAGGAG TGACGTTTGC TCCTGGTCAC AGCTGCATGG TGAGCAACAG AGGACCAGAA
    CCAGCTCCAT ACCCGGATCT CTGTAGAAGT CAGGACCAGA ATCACAGTCA GGACCAGAAT
    CACAGTCAGG ACCAGGACTA CGACCAAATT CACAGTCAGG ACGAGGTTCA GGTCCAGGTC
    CAGGACCAGG GGCCTCATTT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023812931.1
    CDS915..5498
    Translation

    Target ORF information:

    RefSeq Version XM_023957163.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes serine/threonine-protein kinase WNK1 (LOC101162572), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023957163.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGCACT CCGCTGAAGA TGAAGAAGAG GAGAACTCTG CTCTTCATCT CAAGGACTCC 
    GACCAATCAG AGGCTTCCGA TCCACACACA GGTCATGATG TCAGCTCAGA CTCTGCCGAT
    GAAATGGGCA TCAATCTCTC AGAATTAAAC AGTGAGATAT TACCATGGCA ACGCACCCAG
    GAGGAGGAGC ACGACGAAGA GGAAATGAAC GCTGTGGCGT CGTCGCCTGA TGGGCGGTTC
    CTGAAGTTCA ACATCGAGAT CGGGAGGGGC TCCTTCAAAA CCGTTTACAA AGGGCTGGAC
    ACGGAGACCA CAGTGGAGGT GGCCTGGTGC GAGCTCCAGA CCCACCGGCT GAGCAGGGCC
    GAGCGTCAGC GTTTCAGCGA GGAGGTGGAG ATGCTCAAAG CGCTTCAGCA CCCCAACATC
    GTCCGTTTCT TCGATTCCTG GAAGTCCGCG CTGAAGGGCC ACAAATGCAC CATCCTGGTG
    ACGGAGCTCA TGACGTCTGG AACGCTGAAG ATGTACCTGC GCAGGTTCCG TCAGATGAAG
    CTGAAGCTGC TGCAGCGCTG GAGCTTCCAG GTCCTGCGGG GGCTTCACTT CCTGCACTCA
    CGTTCCCCCC CCATCATACA CCGTGACCTC AAATGTGACA ACATCTTCAT CACGGGTCCC
    TCCGCCTCCG TCAAAATCGG AGACCTGGGC CTGGCAACGC TGAAGAAGGC CTCGTTTGCT
    AAGAGCGTGA TCGGAACGCC GGAGTTCATG GCTCCAGAGA TGTACGAGGA GAAGTACGAC
    GAGGCTGTGG ACGTGTACGC CTTCGGCATG TGCATGCTGG AGATGGCTAC GTCCGAGTAT
    CCGTACCACG AGTGTCACAG CGCTGCCCAG ATCTACCGCA AGGTCACCAA TGGAATCAAA
    CCGGACAGTT TCTTCAAAGT GGAAGTTCCA GAACTGAAAG AAATCATTGA AGGCTGCATT
    CGGACCAGGA GCAGTGAAAG GTTTACAGTC CAGGACCTCT TGGAGCACAG CTTCTTTCAG
    GAGAACACCG GCGTCCATGT GGAGCTGGCA GAGGAAGACG ACGGCTCCAA GGCGGCGCTG
    AAGCTGTGGC TCCGGATGGA CGACAAGGAG AAGCTCCGGG GGAAGTATAA AGACAACGAC
    GCCATCGAGT TCCTGTACGA ACTCTACAAG GATGTGCCAG AGGAGGTCGC CCAGGAGATG
    GTCATTTTGG GATTTGTGTT CAAAGCAGAC TACAAGGTGA TTGCCAAAGC CATTCGGCAT
    CGAGTGACAG CCATAAAGTA TCAGCGGGAA AAACGGCGCC TCCTGCTGGA GGAAGGAGTG
    AACAGTCAGA AGCAAACTGA CATCGAGGAA GAGCTTCATC TTCATCAACG AGCTGAGCTG
    TCCAATCAGA CGCTCCCAGT TTCAACAGGA GCCAGAGGAG CGGCCAGTCA GGGGGACTCT
    GCTGCATCCA CACAAGCTCC GCCCCTGACT GCAACAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCTCT GACTGCAACA GCACAAGCTC CGCCCCTGAC TGCATCAGCA
    CAAGCTCCGC CCCTGACTGC AACAGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCAGC ACAAGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGACTGCAT CCACACACGC TCCTTCTCTA ACTGCATCGG CACAAGCTCC GCCCCTGACT
    GCATCAGCAC AAGCTCCGCC CCTGACTGCA TCCACACACG CTCCGCCCCT GACTGCATCA
    GCACAAGCTC CGCCCCTGAT TGCATCCACA CACTCTCCTT CCCTAACTGC ATCGGCACAA
    GCTCCACCCC TGACTGCATC CACACACGCT CCTTCCCTAA CTGCATCGGC ACAAGCTCCG
    CCCCTGACTG CATCGGCACA AACTCCGCCC CTGACTGCAT CGGCACAAGC TCCGCCCCTG
    ACTGCATCAG CACAAGCTCC GCCCCTGACT GCATCCACAC ACGCTCCGCC CCTGACTTTA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CAAGCTCCGC CCAGCAGCCG GGGGGATTCC GGGATCAACA CTGTCTCCAG CAGAGCTGAT
    GGCGACACCG AGGAGGACGC AGACCATGTG ATTGAAACCA CGCCTCTTCT TGTTCCTAAC
    CCCACTCCTC CATCAGTACC TCCACAGACG GAGGTTCCTT CTGTTTTGAC CCGCACAGCT
    TCCAAAGCTC CGCCCTTTCC CATACTACGC TTTCCTAAAA GCATTGCTGT GTCCCAGGCT
    GCAGTAAAGC CCCCCCCTGA GCCCTCCTAT GATTTCTCTT CACCTGTCGA CAGCTGCTCT
    TCTGACTTCA CCTCGGGCTT GAGTGATGGC AATGACGGCC AATCAGAGAG GAGCAGGATG
    GAGGCAACGA TGAGGGAAGC AACCAAACAG TTTAGAAGGA GAGCCAGAGC TCGTCTCCGA
    ATCATAGGGG TGTCTGACAA GGTGGACCGA GTGGTGGAGT GTCAGCTGCA GACTCACAAC
    AACAAGATGG TCACGTTTAA GTTTGACCTG GATGGAGACA ATCCCGAGGA CATCGCATCT
    GTGATGATTC ACAGAGATTT TATCTTACCG TCTGAGCGAG AAGGATTTAT CCTCCGCATG
    TATGACATCA TCCAGAGGGC GGAGTCTATG ATACACCAGC ATCATCCTGC AGTCTTGTAC
    CAGCCTTCTC AGCTGATGAC CTCTGCTCCC CAGTCAAACT GCCTCTGCTT TCAACCTTTT
    CAGCCAAAGT CAGCAGTGAC TAACCTGTCC AGGACTTTGT CTTCCTCCTC CTTACCTGAC
    ATAGATGAAA GTGACACCAC CCCCTCAAAG GCAATTGACT TCAGCATTGA CCCTGATGCT
    GTTCCCCCCG TGAGGCCGTT GAGGTCACAG TCCTTCCAGA CGTCATCAGC CTCTTCCTAT
    GAGCCCCCCC TTTACCCTTA CCATTACCCC CATCCTGAAT CTACCACTCC CCCCCTCTCT
    CAGAATCTGC TTCCTTCTCA TCTTTACTCC ACCTATACAG CCTCTTTCTC CACGCCCAGC
    TCCGCCCCCC AGATTCCACG AGTCCACAGC AACCCCTCCC TCTTTACCTC CACACCATCA
    TCTTTAGCCC CTCCCTCTCC TAGGTGGCCA CCCCCTGACC AGCCTCTCTT CTCTTTGGCC
    AATGTACTGT CTGTGGCCAT GAGTGTGGCC CACAGCCTCA TGCCCCCACC CGGACATCCG
    AGTCAAGGCT TTCAACACCA GGCTCCTCCC CCGCCTTTCC CCCAGTCCAC GTTCTCACCC
    CCCATGTCTC CATCTCTAAA ACGGAACCTT CAGACTTTGA CAAACACCTC CACCGCTGAA
    CCCCACCAAC CCCCCCAGGC CTATCACACA GGCTTGGAGC CAGAAGTCCA AAAGACAGTG
    ATGGACGGCG GAGCTGCAGA TCAGTGTCCC GGTCCTGCCC GACCACGGCT CGGCTCGGCT
    CCCTCCTCCC AGGGTTCAGA GACCTCCGTT CTTCCCCGTC AGGCCAGCCT CTCAAATCCC
    AGAGGAGAGT TTTCAGATTC CTCCACCTCG TCCTCAGCCT CCCCTCCTCC CCCATCGGGT
    TTCTACCCCA AAGTGTCCCC CCCAGTGTCC CCATCACGCT CCATCTCTCC TACGTTGAAA
    GCGGGGAAAA CAGCCTTCAC TGTGGGCCGC TTCCAGGTGA CCCCATCAAC ACCCGTTTCT
    GCTGTCAGTC ATCATCAGCC ACGCCCCTTT AGTGATGCTT CACCCATCGC TCACTCCCCC
    CCTCCTACTC AGAAGAACCA ATCAGAGAGC AGCTCAAGAA GCAGTTCAGA AGAAGACAGC
    CAATCAGAAA TCAGCAACCA CACAGTCATC AATACAACAT ATGGTTACCG TGACAACCCC
    ACCAGTCAAG ACGAGAAGTG CATGAGCAGT CTTAAACTGG GAATGACTAT GTGGGAGGGG
    CCAGCCAACA GCCAATCACG TAGCCGTTCT GCTACTTACA GCTCTGACGA ATCAGAGGGT
    GAGGAGATGT GGGAGGAGCT ACTGGAGCTT CGTAAAAGAC ACTTGGTGGA GGTCCAGAAT
    CTACAGATGA GTCAAAAACA AGAAATGGAG GAGTTGTACA GACGGAAGGG TAAAGCCCTA
    CCACCTGGGA TCGTCCCTCC AGCCGCCATG CTGAACCACC GCCAACGCCG CCTCTCCAAG
    ACAGGAAACT ATCCTCTGGC ACGCCGAAGC AGCCTCCAGA GACTAGATCT GCCGCCCCCT
    GCAGGGATCA TGAGGAAGGG CTCGGTCAGC GGTAGCAGTA GTGGATCTCA AGACCGGATC
    CTGAAAGGAG TGACGTTTGC TCCTGGTCAC AGCTGCATGG TGAGCAACAG AGGACCAGAA
    CCAGCTCCAT ACCCGGATCT CTGTAGAAGT CAGGACCAGA ATCACAGTCA GGACCAGAAT
    CACAGTCAGG ACCAGGACTA CGACCAAATT CACAGTCAGG ACGAGGTTCA GGTCCAGGTC
    CAGGACCAGG GGCCTCATTT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa117517
    Clone ID Related Accession (Same CDS sequence) XM_023957164.1
    Accession Version XM_023957164.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4581bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1518537600000
    Organism Oryzias latipes(Japanese medaka)
    Product serine/threonine-protein kinase WNK1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019866.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGCACT CCGCTGAAGA TGAAGAAGAG GAGAACTCTG CTCTTCATCT CAAGGACTCC 
    GACCAATCAG AGGCTTCCGA TCCACACACA GGTCATGATG TCAGCTCAGA CTCTGCCGAT
    GAAATGGGCA TCAATCTCTC AGAATTAAAC AGTGAGATAT TACCATGGCA ACGCACCCAG
    GAGGAGGAGC ACGACGAAGA GGAAATGAAC GCTGTGGCGT CGTCGCCTGA TGGGCGGTTC
    CTGAAGTTCA ACATCGAGAT CGGGAGGGGC TCCTTCAAAA CCGTTTACAA AGGGCTGGAC
    ACGGAGACCA CAGTGGAGGT GGCCTGGTGC GAGCTCCAGA CCCACCGGCT GAGCAGGGCC
    GAGCGTCAGC GTTTCAGCGA GGAGGTGGAG ATGCTCAAAG CGCTTCAGCA CCCCAACATC
    GTCCGTTTCT TCGATTCCTG GAAGTCCGCG CTGAAGGGCC ACAAATGCAC CATCCTGGTG
    ACGGAGCTCA TGACGTCTGG AACGCTGAAG ATGTACCTGC GCAGGTTCCG TCAGATGAAG
    CTGAAGCTGC TGCAGCGCTG GAGCTTCCAG GTCCTGCGGG GGCTTCACTT CCTGCACTCA
    CGTTCCCCCC CCATCATACA CCGTGACCTC AAATGTGACA ACATCTTCAT CACGGGTCCC
    TCCGCCTCCG TCAAAATCGG AGACCTGGGC CTGGCAACGC TGAAGAAGGC CTCGTTTGCT
    AAGAGCGTGA TCGGAACGCC GGAGTTCATG GCTCCAGAGA TGTACGAGGA GAAGTACGAC
    GAGGCTGTGG ACGTGTACGC CTTCGGCATG TGCATGCTGG AGATGGCTAC GTCCGAGTAT
    CCGTACCACG AGTGTCACAG CGCTGCCCAG ATCTACCGCA AGGTCACCAA TGGAATCAAA
    CCGGACAGTT TCTTCAAAGT GGAAGTTCCA GAACTGAAAG AAATCATTGA AGGCTGCATT
    CGGACCAGGA GCAGTGAAAG GTTTACAGTC CAGGACCTCT TGGAGCACAG CTTCTTTCAG
    GAGAACACCG GCGTCCATGT GGAGCTGGCA GAGGAAGACG ACGGCTCCAA GGCGGCGCTG
    AAGCTGTGGC TCCGGATGGA CGACAAGGAG AAGCTCCGGG GGAAGTATAA AGACAACGAC
    GCCATCGAGT TCCTGTACGA ACTCTACAAG GATGTGCCAG AGGAGGTCGC CCAGGAGATG
    GTCATTTTGG GATTTGTGTT CAAAGCAGAC TACAAGGTGA TTGCCAAAGC CATTCGGCAT
    CGAGTGACAG CCATAAAGTA TCAGCGGGAA AAACGGCGCC TCCTGCTGGA GGAAGGAGTG
    AACAGTCAGA AGCAAACTGA CATCGAGGAA GAGCTTCATC TTCATCAACG AGCTGAGCTG
    TCCAATCAGA CGCTCCCAGT TTCAACAGGA GCCAGAGGAG CGGCCAGTCA GGGGGACTCT
    GCTGCATCCA CACAAGCTCC GCCCCTGACT GCAACAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCTCT GACTGCAACA GCACAAGCTC CGCCCCTGAC TGCATCAGCA
    CAAGCTCCGC CCCTGACTGC AACAGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCAGC ACAAGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGACTGCAT CCACACACGC TCCTTCTCTA ACTGCATCGG CACAAGCTCC GCCCCTGACT
    GCATCAGCAC AAGCTCCGCC CCTGACTGCA TCCACACACG CTCCGCCCCT GACTGCATCA
    GCACAAGCTC CGCCCCTGAT TGCATCCACA CACTCTCCTT CCCTAACTGC ATCGGCACAA
    GCTCCACCCC TGACTGCATC CACACACGCT CCTTCCCTAA CTGCATCGGC ACAAGCTCCG
    CCCCTGACTG CATCGGCACA AACTCCGCCC CTGACTGCAT CGGCACAAGC TCCGCCCCTG
    ACTGCATCAG CACAAGCTCC GCCCCTGACT GCATCCACAC ACGCTCCGCC CCTGACTTTA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CAAGCTCCGC CCAGCAGCCG GGGGGATTCC GGGATCAACA CTGTCTCCAG CAGAGCTGAT
    GGCGACACCG AGGAGGACGA CCATGTGATT GAAACCACGC CTCTTCTTGT TCCTAACCCC
    ACTCCTCCAT CAGTACCTCC ACAGACGGAG GTTCCTTCTG TTTTGACCCG CACAGCTTCC
    AAAGCTCCGC CCTTTCCCAT ACTACGCTTT CCTAAAAGCA TTGCTGTGTC CCAGGCTGCA
    GTAAAGCCCC CCCCTGAGCC CTCCTATGAT TTCTCTTCAC CTGTCGACAG CTGCTCTTCT
    GACTTCACCT CGGGCTTGAG TGATGGCAAT GACGGCCAAT CAGAGAGGAG CAGGATGGAG
    GCAACGATGA GGGAAGCAAC CAAACAGTTT AGAAGGAGAG CCAGAGCTCG TCTCCGAATC
    ATAGGGGTGT CTGACAAGGT GGACCGAGTG GTGGAGTGTC AGCTGCAGAC TCACAACAAC
    AAGATGGTCA CGTTTAAGTT TGACCTGGAT GGAGACAATC CCGAGGACAT CGCATCTGTG
    ATGATTCACA GAGATTTTAT CTTACCGTCT GAGCGAGAAG GATTTATCCT CCGCATGTAT
    GACATCATCC AGAGGGCGGA GTCTATGATA CACCAGCATC ATCCTGCAGT CTTGTACCAG
    CCTTCTCAGC TGATGACCTC TGCTCCCCAG TCAAACTGCC TCTGCTTTCA ACCTTTTCAG
    CCAAAGTCAG CAGTGACTAA CCTGTCCAGG ACTTTGTCTT CCTCCTCCTT ACCTGACATA
    GATGAAAGTG ACACCACCCC CTCAAAGGCA ATTGACTTCA GCATTGACCC TGATGCTGTT
    CCCCCCGTGA GGCCGTTGAG GTCACAGTCC TTCCAGACGT CATCAGCCTC TTCCTATGAG
    CCCCCCCTTT ACCCTTACCA TTACCCCCAT CCTGAATCTA CCACTCCCCC CCTCTCTCAG
    AATCTGCTTC CTTCTCATCT TTACTCCACC TATACAGCCT CTTTCTCCAC GCCCAGCTCC
    GCCCCCCAGA TTCCACGAGT CCACAGCAAC CCCTCCCTCT TTACCTCCAC ACCATCATCT
    TTAGCCCCTC CCTCTCCTAG GTGGCCACCC CCTGACCAGC CTCTCTTCTC TTTGGCCAAT
    GTACTGTCTG TGGCCATGAG TGTGGCCCAC AGCCTCATGC CCCCACCCGG ACATCCGAGT
    CAAGGCTTTC AACACCAGGC TCCTCCCCCG CCTTTCCCCC AGTCCACGTT CTCACCCCCC
    ATGTCTCCAT CTCTAAAACG GAACCTTCAG ACTTTGACAA ACACCTCCAC CGCTGAACCC
    CACCAACCCC CCCAGGCCTA TCACACAGGC TTGGAGCCAG AAGTCCAAAA GACAGTGATG
    GACGGCGGAG CTGCAGATCA GTGTCCCGGT CCTGCCCGAC CACGGCTCGG CTCGGCTCCC
    TCCTCCCAGG GTTCAGAGAC CTCCGTTCTT CCCCGTCAGG CCAGCCTCTC AAATCCCAGA
    GGAGAGTTTT CAGATTCCTC CACCTCGTCC TCAGCCTCCC CTCCTCCCCC ATCGGGTTTC
    TACCCCAAAG TGTCCCCCCC AGTGTCCCCA TCACGCTCCA TCTCTCCTAC GTTGAAAGCG
    GGGAAAACAG CCTTCACTGT GGGCCGCTTC CAGGTGACCC CATCAACACC CGTTTCTGCT
    GTCAGTCATC ATCAGCCACG CCCCTTTAGT GATGCTTCAC CCATCGCTCA CTCCCCCCCT
    CCTACTCAGA AGAACCAATC AGAGAGCAGC TCAAGAAGCA GTTCAGAAGA AGACAGCCAA
    TCAGAAATCA GCAACCACAC AGTCATCAAT ACAACATATG GTTACCGTGA CAACCCCACC
    AGTCAAGACG AGAAGTGCAT GAGCAGTCTT AAACTGGGAA TGACTATGTG GGAGGGGCCA
    GCCAACAGCC AATCACGTAG CCGTTCTGCT ACTTACAGCT CTGACGAATC AGAGGGTGAG
    GAGATGTGGG AGGAGCTACT GGAGCTTCGT AAAAGACACT TGGTGGAGGT CCAGAATCTA
    CAGATGAGTC AAAAACAAGA AATGGAGGAG TTGTACAGAC GGAAGGGTAA AGCCCTACCA
    CCTGGGATCG TCCCTCCAGC CGCCATGCTG AACCACCGCC AACGCCGCCT CTCCAAGACA
    GGAAACTATC CTCTGGCACG CCGAAGCAGC CTCCAGAGAC TAGATCTGCC GCCCCCTGCA
    GGGATCATGA GGAAGGGCTC GGTCAGCGGT AGCAGTAGTG GATCTCAAGA CCGGATCCTG
    AAAGGAGTGA CGTTTGCTCC TGGTCACAGC TGCATGGTGA GCAACAGAGG ACCAGAACCA
    GCTCCATACC CGGATCTCTG TAGAAGTCAG GACCAGAATC ACAGTCAGGA CCAGAATCAC
    AGTCAGGACC AGGACTACGA CCAAATTCAC AGTCAGGACG AGGTTCAGGT CCAGGTCCAG
    GACCAGGGGC CTCATTTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023812932.1
    CDS917..5497
    Translation

    Target ORF information:

    RefSeq Version XM_023957164.1
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes serine/threonine-protein kinase WNK1 (LOC101162572), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023957164.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGCACT CCGCTGAAGA TGAAGAAGAG GAGAACTCTG CTCTTCATCT CAAGGACTCC 
    GACCAATCAG AGGCTTCCGA TCCACACACA GGTCATGATG TCAGCTCAGA CTCTGCCGAT
    GAAATGGGCA TCAATCTCTC AGAATTAAAC AGTGAGATAT TACCATGGCA ACGCACCCAG
    GAGGAGGAGC ACGACGAAGA GGAAATGAAC GCTGTGGCGT CGTCGCCTGA TGGGCGGTTC
    CTGAAGTTCA ACATCGAGAT CGGGAGGGGC TCCTTCAAAA CCGTTTACAA AGGGCTGGAC
    ACGGAGACCA CAGTGGAGGT GGCCTGGTGC GAGCTCCAGA CCCACCGGCT GAGCAGGGCC
    GAGCGTCAGC GTTTCAGCGA GGAGGTGGAG ATGCTCAAAG CGCTTCAGCA CCCCAACATC
    GTCCGTTTCT TCGATTCCTG GAAGTCCGCG CTGAAGGGCC ACAAATGCAC CATCCTGGTG
    ACGGAGCTCA TGACGTCTGG AACGCTGAAG ATGTACCTGC GCAGGTTCCG TCAGATGAAG
    CTGAAGCTGC TGCAGCGCTG GAGCTTCCAG GTCCTGCGGG GGCTTCACTT CCTGCACTCA
    CGTTCCCCCC CCATCATACA CCGTGACCTC AAATGTGACA ACATCTTCAT CACGGGTCCC
    TCCGCCTCCG TCAAAATCGG AGACCTGGGC CTGGCAACGC TGAAGAAGGC CTCGTTTGCT
    AAGAGCGTGA TCGGAACGCC GGAGTTCATG GCTCCAGAGA TGTACGAGGA GAAGTACGAC
    GAGGCTGTGG ACGTGTACGC CTTCGGCATG TGCATGCTGG AGATGGCTAC GTCCGAGTAT
    CCGTACCACG AGTGTCACAG CGCTGCCCAG ATCTACCGCA AGGTCACCAA TGGAATCAAA
    CCGGACAGTT TCTTCAAAGT GGAAGTTCCA GAACTGAAAG AAATCATTGA AGGCTGCATT
    CGGACCAGGA GCAGTGAAAG GTTTACAGTC CAGGACCTCT TGGAGCACAG CTTCTTTCAG
    GAGAACACCG GCGTCCATGT GGAGCTGGCA GAGGAAGACG ACGGCTCCAA GGCGGCGCTG
    AAGCTGTGGC TCCGGATGGA CGACAAGGAG AAGCTCCGGG GGAAGTATAA AGACAACGAC
    GCCATCGAGT TCCTGTACGA ACTCTACAAG GATGTGCCAG AGGAGGTCGC CCAGGAGATG
    GTCATTTTGG GATTTGTGTT CAAAGCAGAC TACAAGGTGA TTGCCAAAGC CATTCGGCAT
    CGAGTGACAG CCATAAAGTA TCAGCGGGAA AAACGGCGCC TCCTGCTGGA GGAAGGAGTG
    AACAGTCAGA AGCAAACTGA CATCGAGGAA GAGCTTCATC TTCATCAACG AGCTGAGCTG
    TCCAATCAGA CGCTCCCAGT TTCAACAGGA GCCAGAGGAG CGGCCAGTCA GGGGGACTCT
    GCTGCATCCA CACAAGCTCC GCCCCTGACT GCAACAGCAC AAGCTCCGCC CCTGACTGCA
    TCAGCACAAG CTCCGCCTCT GACTGCAACA GCACAAGCTC CGCCCCTGAC TGCATCAGCA
    CAAGCTCCGC CCCTGACTGC AACAGCACAA GCTCCGCCCC TGACTGCATC AGCACAAGCT
    CCGCCCCTGA CTGCATCAGC ACAAGCTCCG CCCCTGACTG CATCAGCACA AGCTCCGCCC
    CTGACTGCAT CCACACACGC TCCTTCTCTA ACTGCATCGG CACAAGCTCC GCCCCTGACT
    GCATCAGCAC AAGCTCCGCC CCTGACTGCA TCCACACACG CTCCGCCCCT GACTGCATCA
    GCACAAGCTC CGCCCCTGAT TGCATCCACA CACTCTCCTT CCCTAACTGC ATCGGCACAA
    GCTCCACCCC TGACTGCATC CACACACGCT CCTTCCCTAA CTGCATCGGC ACAAGCTCCG
    CCCCTGACTG CATCGGCACA AACTCCGCCC CTGACTGCAT CGGCACAAGC TCCGCCCCTG
    ACTGCATCAG CACAAGCTCC GCCCCTGACT GCATCCACAC ACGCTCCGCC CCTGACTTTA
    TCAGCACAAG CTCCGCCCCT GACTGCATCA GCACAAGCTC CGCCCCTGAC TGCATCCACA
    CAAGCTCCGC CCAGCAGCCG GGGGGATTCC GGGATCAACA CTGTCTCCAG CAGAGCTGAT
    GGCGACACCG AGGAGGACGA CCATGTGATT GAAACCACGC CTCTTCTTGT TCCTAACCCC
    ACTCCTCCAT CAGTACCTCC ACAGACGGAG GTTCCTTCTG TTTTGACCCG CACAGCTTCC
    AAAGCTCCGC CCTTTCCCAT ACTACGCTTT CCTAAAAGCA TTGCTGTGTC CCAGGCTGCA
    GTAAAGCCCC CCCCTGAGCC CTCCTATGAT TTCTCTTCAC CTGTCGACAG CTGCTCTTCT
    GACTTCACCT CGGGCTTGAG TGATGGCAAT GACGGCCAAT CAGAGAGGAG CAGGATGGAG
    GCAACGATGA GGGAAGCAAC CAAACAGTTT AGAAGGAGAG CCAGAGCTCG TCTCCGAATC
    ATAGGGGTGT CTGACAAGGT GGACCGAGTG GTGGAGTGTC AGCTGCAGAC TCACAACAAC
    AAGATGGTCA CGTTTAAGTT TGACCTGGAT GGAGACAATC CCGAGGACAT CGCATCTGTG
    ATGATTCACA GAGATTTTAT CTTACCGTCT GAGCGAGAAG GATTTATCCT CCGCATGTAT
    GACATCATCC AGAGGGCGGA GTCTATGATA CACCAGCATC ATCCTGCAGT CTTGTACCAG
    CCTTCTCAGC TGATGACCTC TGCTCCCCAG TCAAACTGCC TCTGCTTTCA ACCTTTTCAG
    CCAAAGTCAG CAGTGACTAA CCTGTCCAGG ACTTTGTCTT CCTCCTCCTT ACCTGACATA
    GATGAAAGTG ACACCACCCC CTCAAAGGCA ATTGACTTCA GCATTGACCC TGATGCTGTT
    CCCCCCGTGA GGCCGTTGAG GTCACAGTCC TTCCAGACGT CATCAGCCTC TTCCTATGAG
    CCCCCCCTTT ACCCTTACCA TTACCCCCAT CCTGAATCTA CCACTCCCCC CCTCTCTCAG
    AATCTGCTTC CTTCTCATCT TTACTCCACC TATACAGCCT CTTTCTCCAC GCCCAGCTCC
    GCCCCCCAGA TTCCACGAGT CCACAGCAAC CCCTCCCTCT TTACCTCCAC ACCATCATCT
    TTAGCCCCTC CCTCTCCTAG GTGGCCACCC CCTGACCAGC CTCTCTTCTC TTTGGCCAAT
    GTACTGTCTG TGGCCATGAG TGTGGCCCAC AGCCTCATGC CCCCACCCGG ACATCCGAGT
    CAAGGCTTTC AACACCAGGC TCCTCCCCCG CCTTTCCCCC AGTCCACGTT CTCACCCCCC
    ATGTCTCCAT CTCTAAAACG GAACCTTCAG ACTTTGACAA ACACCTCCAC CGCTGAACCC
    CACCAACCCC CCCAGGCCTA TCACACAGGC TTGGAGCCAG AAGTCCAAAA GACAGTGATG
    GACGGCGGAG CTGCAGATCA GTGTCCCGGT CCTGCCCGAC CACGGCTCGG CTCGGCTCCC
    TCCTCCCAGG GTTCAGAGAC CTCCGTTCTT CCCCGTCAGG CCAGCCTCTC AAATCCCAGA
    GGAGAGTTTT CAGATTCCTC CACCTCGTCC TCAGCCTCCC CTCCTCCCCC ATCGGGTTTC
    TACCCCAAAG TGTCCCCCCC AGTGTCCCCA TCACGCTCCA TCTCTCCTAC GTTGAAAGCG
    GGGAAAACAG CCTTCACTGT GGGCCGCTTC CAGGTGACCC CATCAACACC CGTTTCTGCT
    GTCAGTCATC ATCAGCCACG CCCCTTTAGT GATGCTTCAC CCATCGCTCA CTCCCCCCCT
    CCTACTCAGA AGAACCAATC AGAGAGCAGC TCAAGAAGCA GTTCAGAAGA AGACAGCCAA
    TCAGAAATCA GCAACCACAC AGTCATCAAT ACAACATATG GTTACCGTGA CAACCCCACC
    AGTCAAGACG AGAAGTGCAT GAGCAGTCTT AAACTGGGAA TGACTATGTG GGAGGGGCCA
    GCCAACAGCC AATCACGTAG CCGTTCTGCT ACTTACAGCT CTGACGAATC AGAGGGTGAG
    GAGATGTGGG AGGAGCTACT GGAGCTTCGT AAAAGACACT TGGTGGAGGT CCAGAATCTA
    CAGATGAGTC AAAAACAAGA AATGGAGGAG TTGTACAGAC GGAAGGGTAA AGCCCTACCA
    CCTGGGATCG TCCCTCCAGC CGCCATGCTG AACCACCGCC AACGCCGCCT CTCCAAGACA
    GGAAACTATC CTCTGGCACG CCGAAGCAGC CTCCAGAGAC TAGATCTGCC GCCCCCTGCA
    GGGATCATGA GGAAGGGCTC GGTCAGCGGT AGCAGTAGTG GATCTCAAGA CCGGATCCTG
    AAAGGAGTGA CGTTTGCTCC TGGTCACAGC TGCATGGTGA GCAACAGAGG ACCAGAACCA
    GCTCCATACC CGGATCTCTG TAGAAGTCAG GACCAGAATC ACAGTCAGGA CCAGAATCAC
    AGTCAGGACC AGGACTACGA CCAAATTCAC AGTCAGGACG AGGTTCAGGT CCAGGTCCAG
    GACCAGGGGC CTCATTTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.