LOC101160056 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101160056
Entrez Gene ID	101160056
Full Name	partitioning defective 3 homolog B-like
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020702144.1	XP_020557803.1	partitioning defective 3 homolog B-like isoform X4
XM_020702142.1	XP_020557801.1	partitioning defective 3 homolog B-like isoform X1
XM_011473669.2	XP_011471971.2	partitioning defective 3 homolog B-like isoform X3
XM_020702143.1	XP_020557802.1	partitioning defective 3 homolog B-like isoform X2
XM_023950478.1	XP_023806246.1	partitioning defective 3 homolog B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC101160056 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101160056 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa112989	XM_023950478.1 Latest version!	Oryzias latipes partitioning defective 3 homolog B (LOC101160056), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OJa69322	XM_020702142.1 Latest version!	Oryzias latipes partitioning defective 3 homolog B-like (LOC101160056), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OJa69323	XM_020702143.1 Latest version!	Oryzias latipes partitioning defective 3 homolog B-like (LOC101160056), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OJa69324	XM_011473669.2 Latest version!	Oryzias latipes partitioning defective 3 homolog B-like (LOC101160056), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OJa69325	XM_020702144.1 Latest version!	Oryzias latipes partitioning defective 3 homolog B-like (LOC101160056), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101160056 ( XM_023950478.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023806246.1 Oryzias latipes partitioning defective 3 homolog B (LOC101160056), mRNA.

Price & Availability↑

CloneID	OJa112989
Clone ID Related Accession (Same CDS sequence)	XM_023950478.1
Accession Version	XM_023950478.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	partitioning defective 3 homolog B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019879.2) and transcript sequence (GFIO01015726.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 689 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAAGATGA AGGTGACGGT GACGTTCGGA CAGACAGGTG TGGTGGTCCC CTGTAAGGAG 
GGCTGGACGG TGAGGGACCT GATCCAACAG GCCACGCAGA GGTACCGGAA GCTGCTGGAG 
CAGGAAGGAG ATGTTCTGGT CCGGACTCAC CATGTTGAAT ACTGCGATGG AGGGATCCTG 
GATCCTGATG ACATTCTTAG TGATCTGGTG GATGACAGGG ACAAGCTGAT GGCGGTGTAT 
GAGGAGCAGG AAGCCCAGCA GACGGCTGTG GCTAACTCGA GGTTGACCCC GAGCCCCGAC 
CTCCACCAAG CTGAGCTTTC TGTCTTCCAA CCAACCGCAA GAGGAGAGAT TGAAGTCAAC 
TCCTCGGCTC TTAAATCCGG GACCCCCCTG TTGGTGAGGA GCAGCAGCGA CTCGGCTCTC 
ACTCCACAAA CAGAGATTGA GGGCTCCTTC AGCCAAGAAA CACCTGTGAA GGCAGCCAGC 
CCACCTGTGG AGAAGCCTGG AGGGCTTGAC GGAGCCAAAC ACGCCTTCAA CGGCAGCCTG 
ACCAGATCGG TGGAGGTCCT GGGAGACGAC AGTCCTCTGG GGATCCACGT GGTTCCTTAC 
TGCTCCTCAC TCAGCGGACG GTTCCTCGGC CTTTATATCC GCGGCGTGGA GGAGAAAAGT 
CGCTCTAGAA AAGAAGGCCT ACTCCAAGAG GACGAGTGCA TCGTCAGAAT CAACAACACT 
GACCTCATGG ACAAAACCTT CAGCCAAGCT CAGGACATCT TCCGTCAGGC GATGCGCTCC 
CCAGTCATCT TTCTGGAAGT CGTGCCCCCC TCCAACAAAG ACAGATATGA AAGGAGTCTG 
ATTGGCCAGC TGTCTGGGAC CAGTTCTGGT GCCACCCACA GCGGTTCCTT CTCTGCCAAG 
GCCAAAGACC CCCCACCACC CATAAAGGCC AAGCCGGTAT TCAAGCCCCC TGAGAATCTG 
AGCCCACGAC AAACCGAAGA CGCCAGCCCT CCTGAGCCAG TGGCTACCCT GAAAGGAAGA 
AGCGAGAGCC CCCTTCTTAG GAAGAGCCCC GCCTTCACCA GCATGGTGAC CAATAAGAGA 
GGAGGACGGA AACTGAGGAT CGACTTAACA AAAGGCTCTG AGGGGCTCGG ATTCACCGTC 
GTAACCAGAG ATTCTTCAGT CCACGGTCCA GGTCCCATCC TCGTCAAGAA CATTCTGCCC 
CGAGGAGCTG CGGTCAAAGA TGGACGCCTT CAGTCCGGAG ACCGGATCCT GGAGGTGAAC 
GGCGTGGACA TCACAGGCGT GGGCCAGGAG GAGCTGGTGT GTCTGTTACG CAGCACGCGG 
CAGGGGGAGA CCGTATCGCT GGGGGTGCTG CGGCAGGAGG ACGTGTTCCT GCCCAGAGAG 
ACGAAGGAGG AGGTGCCCCG CGTGGCGGGA GTCGTCCTGG AGAACGGAAA GGAGCAGCTG 
ATGTTCGAGA TTCCCCTGAA CGACAGCGGC TCGGCCGGAC TCGGCGTCAG CCTCAAGGGG 
AACAAGTCCA GGGAGACCAG GGAAGATCTG GGAATCTTCA TCAAGTCCAT CATCCACGGA 
GGAGCTGCTT ACAAGGACGG GCGTCTGAGG ATCAACGACC AGCTCGTGGC CGTTAACGGA 
GAGTCTCTGC TGGGGCGCTC CAATCACGCC GCCATGGAGA CGCTGCGGCG GTCCATGTCC 
CAGGAGGGGA ACGTCAGAGG GACCATCCAG CTGGTGGTGC TGAGGACTCC CCAGGATCAG 
CACGAGGCGT CGCCCAATCG CTCCTTTGAC AGCTCCTCTG GTCTGTTCGG GTCCATCAGT 
CCAGTTAAAG GAATGCTCAG TCCCATCACA GACTCCACCC AGTCGCCGTT GGTGACCAAC 
GCCATCTATG CTTCCACCGT GGCCAATGGG GGCTACTCTG TGCACATGGA CGATGAGGAC 
GAGGGGGGGC CACTCAGTCA CGGGTTTGAC AGCCAGCCCC CCTACCTGCA GGAAAGGAAA 
AGTTCCTCGA CCTCCGGCTC AGCCCAACCA CAGTCACCCG CCCAGAACCA GAACCAGAAC 
CAGCAGCAGT TCCATCACAT GAAGGCCTCC AAGAGCATGG ACCTGGTGGA GGACGAGAGC 
AACGTTGGAT CTCTGAGCGG AAACGTCTCA GCTCCTCCTA CTCCTGTGGA ACTGGGGCCT 
ACTCTCGGTC TGAAGAAGTC CAGCTCCTTG GAGAGTCTGC AGACCGCCAT TCTAGAGGTC 
AACAGGAAGA ACGAGCTCCT CCCATTCCAC CGGCCGCGCC CAAATATGGT CAGAGGACGA 
GGTTGCAACG AAAGCTTCAG AGCGGCTATT GATAAATCCT ATGACGGCCC GGCTGAGGAT 
GACGAGGACG AAGGTTCAGA CGTGAGCTCC GGCCGGGAAA CGCCTGCCAG CAGCTCCTCG 
CTGCAGGGCG CGGGCGACCA CGCCGAGGAC AGGAGCAAAA AAAGACAAGA AGAAGGCGTC 
GAAAAACAAG AAGAAAGAGA AGAACAAACC CAAAGCAAAG GAGAAGAAGA AGGCAGAGGA 
GCTCGAGGAA ACCAAGAAGA GCAAGAAAAT GGTCTTCGGC CTGTTGAGAT TTGGGAAGAA 
AAAGGAGGAG AAGAAGGAAG CCAAGGCGGC TCTGCAGCGG CAAAAGTCGG ACCTGATGAG 
CGACCACGAG CTGGAAAGGC TGAAGGAAGA GCGAGAGAGA ATCGAGGCGT CCCATCCGGA 
GCTGCAGCAG GGGGGGGCTG GAGCTTCCTC GTTCCCAGAC GTGGAGGACG ACGAGGCCGA 
CCCCAACTAC GCCCGGATTC AGACGTTCAG AGACAGAGAG AGCAGCCCGC TGCCCCAGCC 
GCCTCAAGCG CCGCGCTACG GCATGGCTCC ACCCCCTCCA GCCCAAAGGC CCTCCCCCCA 
GAGCCCACCA CGCTTTCAGG GAGGTCACGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023806246.1
CDS	822..3734
Translation	MKMKVTVTFGQTGVVVPCKEGWTVRDLIQQATQRYRKLLEQEGDVLVRTHHVEYCDGGILDPDDILSDLVDDRDKLMAVYEEQEAQQTAVANSRLTPSPDLHQAELSVFQPTARGEIEVNSSALKSGTPLLVRSSSDSALTPQTEIEGSFSQETPVKAASPPVEKPGGLDGAKHAFNGSLTRSVEVLGDDSPLGIHVVPYCSSLSGRFLGLYIRGVEEKSRSRKEGLLQEDECIVRINNTDLMDKTFSQAQDIFRQAMRSPVIFLEVVPPSNKDRYERSLIGQLSGTSSGATHSGSFSAKAKDPPPPIKAKPVFKPPENLSPRQTEDASPPEPVATLKGRSESPLLRKSPAFTSMVTNKRGGRKLRIDLTKGSEGLGFTVVTRDSSVHGPGPILVKNILPRGAAVKDGRLQSGDRILEVNGVDITGVGQEELVCLLRSTRQGETVSLGVLRQEDVFLPRETKEEVPRVAGVVLENGKEQLMFEIPLNDSGSAGLGVSLKGNKSRETREDLGIFIKSIIHGGAAYKDGRLRINDQLVAVNGESLLGRSNHAAMETLRRSMSQEGNVRGTIQLVVLRTPQDQHEASPNRSFDSSSGLFGSISPVKGMLSPITDSTQSPLVTNAIYASTVANGGYSVHMDDEDEGGPLSHGFDSQPPYLQERKSSSTSGSAQPQSPAQNQNQNQQQFHHMKASKSMDLVEDESNVGSLSGNVSAPPTPVELGPTLGLKKSSSLESLQTAILEVNRKNELLPFHRPRPNMVRGRGCNESFRAAIDKSYDGPAEDDEDEGSDVSSGRETPASSSSLQGAGDHAEDRSKKRQEEGVEKQEEREEQTQSKGEEEGRGARGNQEEQENGLRPVEIWEEKGGEEGSQGGSAAAKVGPDERPRAGKAEGRARENRGVPSGAAAGGGWSFLVPRRGGRRGRPQLRPDSDVQRQREQPAAPAASSAALRHGSTPSSPKALPPEPTTLSGRSR

Target ORF information:

RefSeq Version	XM_023950478.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes partitioning defective 3 homolog B (LOC101160056), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023950478.1

ORF Insert Sequence:

ATGAAGATGA AGGTGACGGT GACGTTCGGA CAGACAGGTG TGGTGGTCCC CTGTAAGGAG 
GGCTGGACGG TGAGGGACCT GATCCAACAG GCCACGCAGA GGTACCGGAA GCTGCTGGAG 
CAGGAAGGAG ATGTTCTGGT CCGGACTCAC CATGTTGAAT ACTGCGATGG AGGGATCCTG 
GATCCTGATG ACATTCTTAG TGATCTGGTG GATGACAGGG ACAAGCTGAT GGCGGTGTAT 
GAGGAGCAGG AAGCCCAGCA GACGGCTGTG GCTAACTCGA GGTTGACCCC GAGCCCCGAC 
CTCCACCAAG CTGAGCTTTC TGTCTTCCAA CCAACCGCAA GAGGAGAGAT TGAAGTCAAC 
TCCTCGGCTC TTAAATCCGG GACCCCCCTG TTGGTGAGGA GCAGCAGCGA CTCGGCTCTC 
ACTCCACAAA CAGAGATTGA GGGCTCCTTC AGCCAAGAAA CACCTGTGAA GGCAGCCAGC 
CCACCTGTGG AGAAGCCTGG AGGGCTTGAC GGAGCCAAAC ACGCCTTCAA CGGCAGCCTG 
ACCAGATCGG TGGAGGTCCT GGGAGACGAC AGTCCTCTGG GGATCCACGT GGTTCCTTAC 
TGCTCCTCAC TCAGCGGACG GTTCCTCGGC CTTTATATCC GCGGCGTGGA GGAGAAAAGT 
CGCTCTAGAA AAGAAGGCCT ACTCCAAGAG GACGAGTGCA TCGTCAGAAT CAACAACACT 
GACCTCATGG ACAAAACCTT CAGCCAAGCT CAGGACATCT TCCGTCAGGC GATGCGCTCC 
CCAGTCATCT TTCTGGAAGT CGTGCCCCCC TCCAACAAAG ACAGATATGA AAGGAGTCTG 
ATTGGCCAGC TGTCTGGGAC CAGTTCTGGT GCCACCCACA GCGGTTCCTT CTCTGCCAAG 
GCCAAAGACC CCCCACCACC CATAAAGGCC AAGCCGGTAT TCAAGCCCCC TGAGAATCTG 
AGCCCACGAC AAACCGAAGA CGCCAGCCCT CCTGAGCCAG TGGCTACCCT GAAAGGAAGA 
AGCGAGAGCC CCCTTCTTAG GAAGAGCCCC GCCTTCACCA GCATGGTGAC CAATAAGAGA 
GGAGGACGGA AACTGAGGAT CGACTTAACA AAAGGCTCTG AGGGGCTCGG ATTCACCGTC 
GTAACCAGAG ATTCTTCAGT CCACGGTCCA GGTCCCATCC TCGTCAAGAA CATTCTGCCC 
CGAGGAGCTG CGGTCAAAGA TGGACGCCTT CAGTCCGGAG ACCGGATCCT GGAGGTGAAC 
GGCGTGGACA TCACAGGCGT GGGCCAGGAG GAGCTGGTGT GTCTGTTACG CAGCACGCGG 
CAGGGGGAGA CCGTATCGCT GGGGGTGCTG CGGCAGGAGG ACGTGTTCCT GCCCAGAGAG 
ACGAAGGAGG AGGTGCCCCG CGTGGCGGGA GTCGTCCTGG AGAACGGAAA GGAGCAGCTG 
ATGTTCGAGA TTCCCCTGAA CGACAGCGGC TCGGCCGGAC TCGGCGTCAG CCTCAAGGGG 
AACAAGTCCA GGGAGACCAG GGAAGATCTG GGAATCTTCA TCAAGTCCAT CATCCACGGA 
GGAGCTGCTT ACAAGGACGG GCGTCTGAGG ATCAACGACC AGCTCGTGGC CGTTAACGGA 
GAGTCTCTGC TGGGGCGCTC CAATCACGCC GCCATGGAGA CGCTGCGGCG GTCCATGTCC 
CAGGAGGGGA ACGTCAGAGG GACCATCCAG CTGGTGGTGC TGAGGACTCC CCAGGATCAG 
CACGAGGCGT CGCCCAATCG CTCCTTTGAC AGCTCCTCTG GTCTGTTCGG GTCCATCAGT 
CCAGTTAAAG GAATGCTCAG TCCCATCACA GACTCCACCC AGTCGCCGTT GGTGACCAAC 
GCCATCTATG CTTCCACCGT GGCCAATGGG GGCTACTCTG TGCACATGGA CGATGAGGAC 
GAGGGGGGGC CACTCAGTCA CGGGTTTGAC AGCCAGCCCC CCTACCTGCA GGAAAGGAAA 
AGTTCCTCGA CCTCCGGCTC AGCCCAACCA CAGTCACCCG CCCAGAACCA GAACCAGAAC 
CAGCAGCAGT TCCATCACAT GAAGGCCTCC AAGAGCATGG ACCTGGTGGA GGACGAGAGC 
AACGTTGGAT CTCTGAGCGG AAACGTCTCA GCTCCTCCTA CTCCTGTGGA ACTGGGGCCT 
ACTCTCGGTC TGAAGAAGTC CAGCTCCTTG GAGAGTCTGC AGACCGCCAT TCTAGAGGTC 
AACAGGAAGA ACGAGCTCCT CCCATTCCAC CGGCCGCGCC CAAATATGGT CAGAGGACGA 
GGTTGCAACG AAAGCTTCAG AGCGGCTATT GATAAATCCT ATGACGGCCC GGCTGAGGAT 
GACGAGGACG AAGGTTCAGA CGTGAGCTCC GGCCGGGAAA CGCCTGCCAG CAGCTCCTCG 
CTGCAGGGCG CGGGCGACCA CGCCGAGGAC AGGAGCAAAA AAAGACAAGA AGAAGGCGTC 
GAAAAACAAG AAGAAAGAGA AGAACAAACC CAAAGCAAAG GAGAAGAAGA AGGCAGAGGA 
GCTCGAGGAA ACCAAGAAGA GCAAGAAAAT GGTCTTCGGC CTGTTGAGAT TTGGGAAGAA 
AAAGGAGGAG AAGAAGGAAG CCAAGGCGGC TCTGCAGCGG CAAAAGTCGG ACCTGATGAG 
CGACCACGAG CTGGAAAGGC TGAAGGAAGA GCGAGAGAGA ATCGAGGCGT CCCATCCGGA 
GCTGCAGCAG GGGGGGGCTG GAGCTTCCTC GTTCCCAGAC GTGGAGGACG ACGAGGCCGA 
CCCCAACTAC GCCCGGATTC AGACGTTCAG AGACAGAGAG AGCAGCCCGC TGCCCCAGCC 
GCCTCAAGCG CCGCGCTACG GCATGGCTCC ACCCCCTCCA GCCCAAAGGC CCTCCCCCCA 
GAGCCCACCA CGCTTTCAGG GAGGTCACGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101160056 ( XM_020702142.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020557801.1 Oryzias latipes partitioning defective 3 homolog B-like (LOC101160056), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OJa69322
Clone ID Related Accession (Same CDS sequence)	XM_020702142.1
Accession Version	XM_020702142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	partitioning defective 3 homolog B-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004095217.1) and transcript sequence (GFIO01015727.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1422 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAAGATGA AGGTGACGGT GACGTTCGGA CAGACAGGTG TGGTGGTCCC CTGTAAGGAG 
GGCTGGACGG TGAGGGACCT GATCCAACAG GCCACGCAGA GGTACCGGAA GCTGCTGGAG 
CAGGAAGGAG ATGTTCTGGT CCGGACTCAC CATGTTGAAT ACTGCGATGG AGGGATCCTG 
GATCCTGATG ACATTCTTAG TGATCTGGTG GATGACAGGG ACAAGCTGAT GGCGGTGTAT 
GAGGAGCAGG AAGCCCAGCA GACGGCTGTG GCTAACTCGA GGTTGACCCC GAGCCCCGAC 
CTCCACCAAG CTGAGCTTTC TGTCTTCCAA CCAACCGCAA GAGGAGAGAT TGAAGTCAAC 
TCCTCGGCTC TTAAATCCGG GACCCCCCTG TTGGTGAGGA GCAGCAGCGA CTCGGCTCTC 
ACTCCACAAA CAGAGATTGA GGGCTCCTTC AGCCAAGAAA CACCTGTGAA GGCAGCCAGC 
CCACCTGTGG AGAAGCCTGG AGGGCTTGAC GGAGCCAAAC ACGCCTTCAA CGGCAGCCTG 
ACCAGATCGG TGGAGGTCCT GGGAGACGAC AGTCCTCTGG GGATCCACGT GGTTCCTTAC 
TGCTCCTCAC TCAGCGGACG GTTCCTCGGC CTTTATATCC GCGGCGTGGA GGAGAAAAGT 
CGCTCTAGAA AAGAAGGCCT ACTCCAAGAG GACGAGTGCA TCGTCAGAAT CAACAACACT 
GACCTCATGG ACAAAACCTT CAGCCAAGCT CAGGACATCT TCCGTCAGGC GATGCGCTCC 
CCAGTCATCT TTCTGGAAGT CGTGCCCCCC TCCAACAAAG ACAGATATGA AAGGAGTCTG 
ATTGGCCAGC TGTCTGGGAC CAGTTCTGGT GCCACCCACA GCGGTTCCTT CTCTGCCAAG 
GCCAAAGACC CCCCACCACC CATAAAGGCC AAGCCGGTAT TCAAGCCCCC TGAGAATCTG 
AGCCCACGAC AAACCGAAGA CGCCAGCCCT CCTGAGCCAG TGGCTACCCT GAAAGGAAGA 
AGCGAGAGCC CCCTTCTTAG GAAGAGCCCC GCCTTCACCA GCATGGTGAC CAATAAGAGA 
GGAGGACGGA AACTGAGGAT CGACTTAACA AAAGGCTCTG AGGGGCTCGG ATTCACCGTC 
GTAACCAGAG ATTCTTCAGT CCACGGTCCA GGTCCCATCC TCGTCAAGAA CATTCTGCCC 
CGAGGAGCTG CGGTCAAAGA TGGACGCCTT CAGTCCGGAG ACCGGATCCT GGAGGTGAAC 
GGCGTGGACA TCACAGGCGT GGGCCAGGAG GAGCTGGTGT GTCTGTTACG CAGCACGCGG 
CAGGGGGAGA CCGTATCGCT GGGGGTGCTG CGGCAGGAGG ACGTGTTCCT GCCCAGAGAG 
ACGAAGGAGG AGGTGCCCCG CGTGGCGGGA GTCGTCCTGG AGAACGGAAA GGAGCAGCTG 
ATGTTCGAGA TTCCCCTGAA CGACAGCGGC TCGGCCGGAC TCGGCGTCAG CCTCAAGGGG 
AACAAGTCCA GGGAGACCAG GGAAGATCTG GGAATCTTCA TCAAGTCCAT CATCCACGGA 
GGAGCTGCTT ACAAGGACGG GCGTCTGAGG ATCAACGACC AGCTCGTGGC CGTTAACGGA 
GAGTCTCTGC TGGGGCGCTC CAATCACGCC GCCATGGAGA CGCTGCGGCG GTCCATGTCC 
CAGGAGGGGA ACGTCAGAGG GACCATCCAG CTGGTGGTGC TGAGGACTCC CCAGGATCAG 
CACGAGGCGT CGCCCAATCG CTCCTTTGAC AGCTCCTCTG GTCTGTTCGG GTCCATCAGT 
CCAGTTAAAG GAATGCTCAG TCCCATCACA GACTCCACCC AGTCGCCGTT GGTGACCAAC 
GCCATCTATG CTTCCACCGT GGCCAATGGG GGCTACTCTG TGCACATGGA CGATGAGGAC 
GAGGGGGGGC CACTCAGTCA CGGGTTTGAC AGCCAGCCCC CCTACCTGCA GGAAAGGAAA 
AGTTCCTCGA CCTCCGGCTC AGCCCAACCA CAGTCACCCG CCCAGAACCA GAACCAGAAC 
CAGCAGCAGT TCCATCACAT GAAGGCCTCC AAGAGCATGG ACCTGGGTAC GAGTCAGAGC 
CAGCGGCCCA TCGTGGAGGA CGAGAGCAAC GTTGGATCTC TGAGCGGAAA CGTCTCAGCT 
CCTCCTACTC CTGTGGAACT GGGGCCTACT CTCGGTCTGA AGAAGTCCAG CTCCTTGGAG 
AGTCTGCAGA CCGCCATTCT AGAGGTCAAC AGGAAGAACG AGCTCCTCCC ATTCCACCGG 
CCGCGCCCAA ATATGGTCAG AGGACGAGGT TGCAACGAAA GCTTCAGAGC GGCTATTGAT 
AAATCCTATG ACGGCCCGGC TGAGGATGAC GAGGACGAAG GTTCAGACGT GAGCTCCGGC 
CGGGAAACGC CTGCCAGCAG CTCCTCGCTG CAGGGCGCGG GCGACCACGC CGAGGACAGG 
AGCAAAAAAG ACAAGAAGAA GGCGTCGAAA AACAAGAAGA AAGAGAAGAA CAAACCCAAA 
GCAAAGGAGA AGAAGAAGGC AGAGGAGCTC GAGGAAACCA AGAAGAGCAA GAAAATGGTC 
TTCGGCCTGT TGAGATTTGG GAAGAAAAAG GAGGAGAAGA AGGAAGCCAA GGCGGCTCTG 
CAGCGGCAAA AGTCGGACCT GATGAGCGAC CACGAGCTGG AAAGGCTGAA GGAAGAGCGA 
GAGAGAATCG AGGCGTCCCA TCCGGAGCTG CAGCAGGGGG GGGCTGGAGC TTCCTCGTTC 
CCAGACGTGG AGGACGACGA GGCCGACCCC AACTACGCCC GGATTCAGAC GTTCAGAGAC 
AGAGAGAGCA GCCCGCTGCC CCAGCCGCCT CAAGCGCCGC GCTACGGCAT GGCTCCACCC 
CCTCCAGCCC AAAGGCCCTC CCCCCAGAGC CCACCACGCT TTCAGGGAGG TCACGCTGAA 
GACGCAGGTG CCGTCCCTGA CGATGAGCAC GTGGACAGGC TGTACGCCAA AGTCAACAAG 
CAGAGGAGCG CCGGGGCGGC GTCCCCTGCC ACCACCCACA GCAGCACCGA CCGGATCCAG 
CAGCTCAGGA GAGAGTATCA GCAGGCCAGG CGGGAAGGGG CGGCGCCGCC GTACGAGGAG 
GCGGAGCCAC GAGTCCGAGC GCATGAAAGC AGCGCTCACA GGATTCCAGG CAGGGGGCCC 
GATCCCCGTG GGACCCCCCG CTATGAGGAG GTGGAGCGTC AGTATGCCTC CTTACCCAGA 
CGCGGCCCGT TGGACCCTGC CGAGTACCCC ATGCAGCCAT GGTCAGGTCT TTATCCTGGA 
CCCCCCCAGA CCCAGCAGGG ATACCCCCAG GCTACCATGT ACCCTGATTC CAACTCCCAG 
TCCGTCCCAA CGTACTCCAC CTTCAAGCCC GGCCCCCCGT ACCCAAGACC CGGGGACCAT 
CGACCCGTGG ACCCTGGATA CTACCCCAAC CCGGGCCCCC AGCAGAGGGG CCCCCTGCGG 
CAGGACGTGC CCCCATCTCC CACCCCTCCA CTCCGTGTGC CCCGCTATGA CACCATGACA 
CGTGGAACTG TGGGAGGAGC CTACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020557801.1
CDS	824..4393
Translation	MKMKVTVTFGQTGVVVPCKEGWTVRDLIQQATQRYRKLLEQEGDVLVRTHHVEYCDGGILDPDDILSDLVDDRDKLMAVYEEQEAQQTAVANSRLTPSPDLHQAELSVFQPTARGEIEVNSSALKSGTPLLVRSSSDSALTPQTEIEGSFSQETPVKAASPPVEKPGGLDGAKHAFNGSLTRSVEVLGDDSPLGIHVVPYCSSLSGRFLGLYIRGVEEKSRSRKEGLLQEDECIVRINNTDLMDKTFSQAQDIFRQAMRSPVIFLEVVPPSNKDRYERSLIGQLSGTSSGATHSGSFSAKAKDPPPPIKAKPVFKPPENLSPRQTEDASPPEPVATLKGRSESPLLRKSPAFTSMVTNKRGGRKLRIDLTKGSEGLGFTVVTRDSSVHGPGPILVKNILPRGAAVKDGRLQSGDRILEVNGVDITGVGQEELVCLLRSTRQGETVSLGVLRQEDVFLPRETKEEVPRVAGVVLENGKEQLMFEIPLNDSGSAGLGVSLKGNKSRETREDLGIFIKSIIHGGAAYKDGRLRINDQLVAVNGESLLGRSNHAAMETLRRSMSQEGNVRGTIQLVVLRTPQDQHEASPNRSFDSSSGLFGSISPVKGMLSPITDSTQSPLVTNAIYASTVANGGYSVHMDDEDEGGPLSHGFDSQPPYLQERKSSSTSGSAQPQSPAQNQNQNQQQFHHMKASKSMDLGTSQSQRPIVEDESNVGSLSGNVSAPPTPVELGPTLGLKKSSSLESLQTAILEVNRKNELLPFHRPRPNMVRGRGCNESFRAAIDKSYDGPAEDDEDEGSDVSSGRETPASSSSLQGAGDHAEDRSKKDKKKASKNKKKEKNKPKAKEKKKAEELEETKKSKKMVFGLLRFGKKKEEKKEAKAALQRQKSDLMSDHELERLKEERERIEASHPELQQGGAGASSFPDVEDDEADPNYARIQTFRDRESSPLPQPPQAPRYGMAPPPPAQRPSPQSPPRFQGGHAEDAGAVPDDEHVDRLYAKVNKQRSAGAASPATTHSSTDRIQQLRREYQQARREGAAPPYEEAEPRVRAHESSAHRIPGRGPDPRGTPRYEEVERQYASLPRRGPLDPAEYPMQPWSGLYPGPPQTQQGYPQATMYPDSNSQSVPTYSTFKPGPPYPRPGDHRPVDPGYYPNPGPQQRGPLRQDVPPSPTPPLRVPRYDTMTRGTVGGAYR

Target ORF information:

RefSeq Version	XM_020702142.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes partitioning defective 3 homolog B-like (LOC101160056), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020702142.1

ORF Insert Sequence:

ATGAAGATGA AGGTGACGGT GACGTTCGGA CAGACAGGTG TGGTGGTCCC CTGTAAGGAG 
GGCTGGACGG TGAGGGACCT GATCCAACAG GCCACGCAGA GGTACCGGAA GCTGCTGGAG 
CAGGAAGGAG ATGTTCTGGT CCGGACTCAC CATGTTGAAT ACTGCGATGG AGGGATCCTG 
GATCCTGATG ACATTCTTAG TGATCTGGTG GATGACAGGG ACAAGCTGAT GGCGGTGTAT 
GAGGAGCAGG AAGCCCAGCA GACGGCTGTG GCTAACTCGA GGTTGACCCC GAGCCCCGAC 
CTCCACCAAG CTGAGCTTTC TGTCTTCCAA CCAACCGCAA GAGGAGAGAT TGAAGTCAAC 
TCCTCGGCTC TTAAATCCGG GACCCCCCTG TTGGTGAGGA GCAGCAGCGA CTCGGCTCTC 
ACTCCACAAA CAGAGATTGA GGGCTCCTTC AGCCAAGAAA CACCTGTGAA GGCAGCCAGC 
CCACCTGTGG AGAAGCCTGG AGGGCTTGAC GGAGCCAAAC ACGCCTTCAA CGGCAGCCTG 
ACCAGATCGG TGGAGGTCCT GGGAGACGAC AGTCCTCTGG GGATCCACGT GGTTCCTTAC 
TGCTCCTCAC TCAGCGGACG GTTCCTCGGC CTTTATATCC GCGGCGTGGA GGAGAAAAGT 
CGCTCTAGAA AAGAAGGCCT ACTCCAAGAG GACGAGTGCA TCGTCAGAAT CAACAACACT 
GACCTCATGG ACAAAACCTT CAGCCAAGCT CAGGACATCT TCCGTCAGGC GATGCGCTCC 
CCAGTCATCT TTCTGGAAGT CGTGCCCCCC TCCAACAAAG ACAGATATGA AAGGAGTCTG 
ATTGGCCAGC TGTCTGGGAC CAGTTCTGGT GCCACCCACA GCGGTTCCTT CTCTGCCAAG 
GCCAAAGACC CCCCACCACC CATAAAGGCC AAGCCGGTAT TCAAGCCCCC TGAGAATCTG 
AGCCCACGAC AAACCGAAGA CGCCAGCCCT CCTGAGCCAG TGGCTACCCT GAAAGGAAGA 
AGCGAGAGCC CCCTTCTTAG GAAGAGCCCC GCCTTCACCA GCATGGTGAC CAATAAGAGA 
GGAGGACGGA AACTGAGGAT CGACTTAACA AAAGGCTCTG AGGGGCTCGG ATTCACCGTC 
GTAACCAGAG ATTCTTCAGT CCACGGTCCA GGTCCCATCC TCGTCAAGAA CATTCTGCCC 
CGAGGAGCTG CGGTCAAAGA TGGACGCCTT CAGTCCGGAG ACCGGATCCT GGAGGTGAAC 
GGCGTGGACA TCACAGGCGT GGGCCAGGAG GAGCTGGTGT GTCTGTTACG CAGCACGCGG 
CAGGGGGAGA CCGTATCGCT GGGGGTGCTG CGGCAGGAGG ACGTGTTCCT GCCCAGAGAG 
ACGAAGGAGG AGGTGCCCCG CGTGGCGGGA GTCGTCCTGG AGAACGGAAA GGAGCAGCTG 
ATGTTCGAGA TTCCCCTGAA CGACAGCGGC TCGGCCGGAC TCGGCGTCAG CCTCAAGGGG 
AACAAGTCCA GGGAGACCAG GGAAGATCTG GGAATCTTCA TCAAGTCCAT CATCCACGGA 
GGAGCTGCTT ACAAGGACGG GCGTCTGAGG ATCAACGACC AGCTCGTGGC CGTTAACGGA 
GAGTCTCTGC TGGGGCGCTC CAATCACGCC GCCATGGAGA CGCTGCGGCG GTCCATGTCC 
CAGGAGGGGA ACGTCAGAGG GACCATCCAG CTGGTGGTGC TGAGGACTCC CCAGGATCAG 
CACGAGGCGT CGCCCAATCG CTCCTTTGAC AGCTCCTCTG GTCTGTTCGG GTCCATCAGT 
CCAGTTAAAG GAATGCTCAG TCCCATCACA GACTCCACCC AGTCGCCGTT GGTGACCAAC 
GCCATCTATG CTTCCACCGT GGCCAATGGG GGCTACTCTG TGCACATGGA CGATGAGGAC 
GAGGGGGGGC CACTCAGTCA CGGGTTTGAC AGCCAGCCCC CCTACCTGCA GGAAAGGAAA 
AGTTCCTCGA CCTCCGGCTC AGCCCAACCA CAGTCACCCG CCCAGAACCA GAACCAGAAC 
CAGCAGCAGT TCCATCACAT GAAGGCCTCC AAGAGCATGG ACCTGGGTAC GAGTCAGAGC 
CAGCGGCCCA TCGTGGAGGA CGAGAGCAAC GTTGGATCTC TGAGCGGAAA CGTCTCAGCT 
CCTCCTACTC CTGTGGAACT GGGGCCTACT CTCGGTCTGA AGAAGTCCAG CTCCTTGGAG 
AGTCTGCAGA CCGCCATTCT AGAGGTCAAC AGGAAGAACG AGCTCCTCCC ATTCCACCGG 
CCGCGCCCAA ATATGGTCAG AGGACGAGGT TGCAACGAAA GCTTCAGAGC GGCTATTGAT 
AAATCCTATG ACGGCCCGGC TGAGGATGAC GAGGACGAAG GTTCAGACGT GAGCTCCGGC 
CGGGAAACGC CTGCCAGCAG CTCCTCGCTG CAGGGCGCGG GCGACCACGC CGAGGACAGG 
AGCAAAAAAG ACAAGAAGAA GGCGTCGAAA AACAAGAAGA AAGAGAAGAA CAAACCCAAA 
GCAAAGGAGA AGAAGAAGGC AGAGGAGCTC GAGGAAACCA AGAAGAGCAA GAAAATGGTC 
TTCGGCCTGT TGAGATTTGG GAAGAAAAAG GAGGAGAAGA AGGAAGCCAA GGCGGCTCTG 
CAGCGGCAAA AGTCGGACCT GATGAGCGAC CACGAGCTGG AAAGGCTGAA GGAAGAGCGA 
GAGAGAATCG AGGCGTCCCA TCCGGAGCTG CAGCAGGGGG GGGCTGGAGC TTCCTCGTTC 
CCAGACGTGG AGGACGACGA GGCCGACCCC AACTACGCCC GGATTCAGAC GTTCAGAGAC 
AGAGAGAGCA GCCCGCTGCC CCAGCCGCCT CAAGCGCCGC GCTACGGCAT GGCTCCACCC 
CCTCCAGCCC AAAGGCCCTC CCCCCAGAGC CCACCACGCT TTCAGGGAGG TCACGCTGAA 
GACGCAGGTG CCGTCCCTGA CGATGAGCAC GTGGACAGGC TGTACGCCAA AGTCAACAAG 
CAGAGGAGCG CCGGGGCGGC GTCCCCTGCC ACCACCCACA GCAGCACCGA CCGGATCCAG 
CAGCTCAGGA GAGAGTATCA GCAGGCCAGG CGGGAAGGGG CGGCGCCGCC GTACGAGGAG 
GCGGAGCCAC GAGTCCGAGC GCATGAAAGC AGCGCTCACA GGATTCCAGG CAGGGGGCCC 
GATCCCCGTG GGACCCCCCG CTATGAGGAG GTGGAGCGTC AGTATGCCTC CTTACCCAGA 
CGCGGCCCGT TGGACCCTGC CGAGTACCCC ATGCAGCCAT GGTCAGGTCT TTATCCTGGA 
CCCCCCCAGA CCCAGCAGGG ATACCCCCAG GCTACCATGT ACCCTGATTC CAACTCCCAG 
TCCGTCCCAA CGTACTCCAC CTTCAAGCCC GGCCCCCCGT ACCCAAGACC CGGGGACCAT 
CGACCCGTGG ACCCTGGATA CTACCCCAAC CCGGGCCCCC AGCAGAGGGG CCCCCTGCGG 
CAGGACGTGC CCCCATCTCC CACCCCTCCA CTCCGTGTGC CCCGCTATGA CACCATGACA 
CGTGGAACTG TGGGAGGAGC CTACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101160056 ( XM_020702143.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020557802.1 Oryzias latipes partitioning defective 3 homolog B-like (LOC101160056), transcript variant X2, mRNA.

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CloneID	OJa69323
Clone ID Related Accession (Same CDS sequence)	XM_020702143.1
Accession Version	XM_020702143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	partitioning defective 3 homolog B-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004095217.1) and transcript sequence (GFIO01015727.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1422 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAAGATGA AGGTGACGGT GACGTTCGGA CAGACAGGTG TGGTGGTCCC CTGTAAGGAG 
GGCTGGACGG TGAGGGACCT GATCCAACAG GCCACGCAGA GGTACCGGAA GCTGCTGGAG 
CAGGAAGGAG ATGTTCTGGT CCGGACTCAC CATGTTGAAT ACTGCGATGG AGGGATCCTG 
GATCCTGATG ACATTCTTAG TGATCTGGTG GATGACAGGG ACAAGCTGAT GGCGGTGTAT 
GAGGAGCAGG AAGCCCAGCA GACGGCTGTG GCTAACTCGA GGTTGACCCC GAGCCCCGAC 
CTCCACCAAG CTGAGCTTTC TGTCTTCCAA CCAACCGCAA GAGGAGAGAT TGAAGTCAAC 
TCCTCGGCTC TTAAATCCGG GACCCCCCTG TTGGTGAGGA GCAGCAGCGA CTCGGCTCTC 
ACTCCACAAA CAGAGATTGA GGGCTCCTTC AGCCAAGAAA CACCTGTGAA GGCAGCCAGC 
CCACCTGTGG AGAAGCCTGG AGGGCTTGAC GGAGCCAAAC ACGCCTTCAA CGGCAGCCTG 
ACCAGATCGG TGGAGGTCCT GGGAGACGAC AGTCCTCTGG GGATCCACGT GGTTCCTTAC 
TGCTCCTCAC TCAGCGGACG GTTCCTCGGC CTTTATATCC GCGGCGTGGA GGAGAAAAGT 
CGCTCTAGAA AAGAAGGCCT ACTCCAAGAG GACGAGTGCA TCGTCAGAAT CAACAACACT 
GACCTCATGG ACAAAACCTT CAGCCAAGCT CAGGACATCT TCCGTCAGGC GATGCGCTCC 
CCAGTCATCT TTCTGGAAGT CGTGCCCCCC TCCAACAAAG ACAGATATGA AAGGAGTCTG 
ATTGGCCAGC TGTCTGGGAC CAGTTCTGGT GCCACCCACA GCGGTTCCTT CTCTGCCAAG 
GCCAAAGACC CCCCACCACC CATAAAGGCC AAGCCGGTAT TCAAGCCCCC TGAGAATCTG 
AGCCCACGAC AAACCGAAGA CGCCAGCCCT CCTGAGCCAG TGGCTACCCT GAAAGGAAGA 
AGCGAGAGCC CCCTTCTTAG GAAGAGCCCC GCCTTCACCA GCATGGTGAC CAATAAGAGA 
GGAGGACGGA AACTGAGGAT CGACTTAACA AAAGGCTCTG AGGGGCTCGG ATTCACCGTC 
GTAACCAGAG ATTCTTCAGT CCACGGTCCA GGTCCCATCC TCGTCAAGAA CATTCTGCCC 
CGAGGAGCTG CGGTCAAAGA TGGACGCCTT CAGTCCGGAG ACCGGATCCT GGAGGTGAAC 
GGCGTGGACA TCACAGGCGT GGGCCAGGAG GAGCTGGTGT GTCTGTTACG CAGCACGCGG 
CAGGGGGAGA CCGTATCGCT GGGGGTGCTG CGGCAGGAGG ACGTGTTCCT GCCCAGAGAG 
ACGAAGGAGG AGGTGCCCCG CGTGGCGGGA GTCGTCCTGG AGAACGGAAA GGAGCAGCTG 
ATGTTCGAGA TTCCCCTGAA CGACAGCGGC TCGGCCGGAC TCGGCGTCAG CCTCAAGGGG 
AACAAGTCCA GGGAGACCAG GGAAGATCTG GGAATCTTCA TCAAGTCCAT CATCCACGGA 
GGAGCTGCTT ACAAGGACGG GCGTCTGAGG ATCAACGACC AGCTCGTGGC CGTTAACGGA 
GAGTCTCTGC TGGGGCGCTC CAATCACGCC GCCATGGAGA CGCTGCGGCG GTCCATGTCC 
CAGGAGGGGA ACGTCAGAGG GACCATCCAG CTGGTGGTGC TGAGGACTCC CCAGGATCAG 
CACGAGGCGT CGCCCAATCG CTCCTTTGAC AGCTCCTCTG GTCTGTTCGG GTCCATCAGT 
CCAGTTAAAG GAATGCTCAG TCCCATCACA GACTCCACCC AGTCGCCGTT GGTGACCAAC 
GCCATCTATG CTTCCACCGT GGCCAATGGG GGCTACTCTG TGCACATGGA CGATGAGGAC 
GAGGGGGGGC CACTCAGTCA CGGGTTTGAC AGCCAGCCCC CCTACCTGCA GGAAAGGAAA 
AGTTCCTCGA CCTCCGGCTC AGCCCAACCA CAGTCACCCG CCCAGAACCA GAACCAGAAC 
CAGCAGCAGT TCCATCACAT GAAGGCCTCC AAGAGCATGG ACCTGGGTAC GAGTCAGAGC 
CAGCGGCCCA TCGTGGAGGA CGAGAGCAAC GTTGGATCTC TGAGCGGAAA CGTCTCAGCT 
CCTCCTACTC CTGTGGAACT GGGGCCTACT CTCGGTCTGA AGAAGTCCAG CTCCTTGGAG 
AGTCTGCAGA CCGCCATTCT AGAGGTCAAC AGGAAGAACG AGCTCCTCCC ATTCCACCGG 
CCGCGCCCAA ATATGGTCAG AGGACGAGGT TGCAACGAAA GCTTCAGAGC GGCTATTGAT 
AAATCCTATG ACGGCCCGGC TGAGGATGAC GAGGACGAAG GTTCAGACGT GAGCTCCGGC 
CGGGAAACGC CTGCCAGCAG CTCCTCGCTG CAGGGCGCGG GCGACCACGC CGAGGACAGG 
AGCAAAAAAG ACAAGAAGAA GGCGTCGAAA AACAAGAAGA AAGAGAAGAA CAAACCCAAA 
GCAAAGGAGA AGAAGAAGGC AGAGGAGCTC GAGGAAACCA AGAAGAGCAA GAAAATGGTC 
TTCGGCCTGT TGAGATTTGG GAAGAAAAAG GAGGAGAAGA AGGAAGCCAA GGCGGCTCTG 
CAGCGGCAAA AGTCGGACCT GATGAGCGAC CACGAGCTGG AAAGGCTGAA GGAAGAGCGA 
GAGAGAATCG AGGCGTCCCA TCCGGAGCTG CAGCAGGGGG GGGCTGGAGC TTCCTCGTTC 
CCAGACGTGG AGGACGACGA GGCCGACCCC AACTACGCCC GGATTCAGAC GTTCAGAGAC 
AGAGAGAGCA GCCCGCTGCC CCAGCCGCCT CAAGCGCCGC GCTACGGCAT GGCTCCACCC 
CCTCCAGCCC AAAGGCCCTC CCCCCAGAGC CCACCACGCT TTCAGGGAGG TCACGCTGAA 
GACGCAGGTG CCGTCCCTGA CGATGAGCAC GTGGACAGGC TGTACGCCAA AGTCAACAAG 
CAGAGGAGCG CCGGGGCGGC GTCCCCTGCC ACCACCCACA GCACCGACCG GATCCAGCAG 
CTCAGGAGAG AGTATCAGCA GGCCAGGCGG GAAGGGGCGG CGCCGCCGTA CGAGGAGGCG 
GAGCCACGAG TCCGAGCGCA TGAAAGCAGC GCTCACAGGA TTCCAGGCAG GGGGCCCGAT 
CCCCGTGGGA CCCCCCGCTA TGAGGAGGTG GAGCGTCAGT ATGCCTCCTT ACCCAGACGC 
GGCCCGTTGG ACCCTGCCGA GTACCCCATG CAGCCATGGT CAGGTCTTTA TCCTGGACCC 
CCCCAGACCC AGCAGGGATA CCCCCAGGCT ACCATGTACC CTGATTCCAA CTCCCAGTCC 
GTCCCAACGT ACTCCACCTT CAAGCCCGGC CCCCCGTACC CAAGACCCGG GGACCATCGA 
CCCGTGGACC CTGGATACTA CCCCAACCCG GGCCCCCAGC AGAGGGGCCC CCTGCGGCAG 
GACGTGCCCC CATCTCCCAC CCCTCCACTC CGTGTGCCCC GCTATGACAC CATGACACGT 
GGAACTGTGG GAGGAGCCTA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020557802.1
CDS	824..4390
Translation	MKMKVTVTFGQTGVVVPCKEGWTVRDLIQQATQRYRKLLEQEGDVLVRTHHVEYCDGGILDPDDILSDLVDDRDKLMAVYEEQEAQQTAVANSRLTPSPDLHQAELSVFQPTARGEIEVNSSALKSGTPLLVRSSSDSALTPQTEIEGSFSQETPVKAASPPVEKPGGLDGAKHAFNGSLTRSVEVLGDDSPLGIHVVPYCSSLSGRFLGLYIRGVEEKSRSRKEGLLQEDECIVRINNTDLMDKTFSQAQDIFRQAMRSPVIFLEVVPPSNKDRYERSLIGQLSGTSSGATHSGSFSAKAKDPPPPIKAKPVFKPPENLSPRQTEDASPPEPVATLKGRSESPLLRKSPAFTSMVTNKRGGRKLRIDLTKGSEGLGFTVVTRDSSVHGPGPILVKNILPRGAAVKDGRLQSGDRILEVNGVDITGVGQEELVCLLRSTRQGETVSLGVLRQEDVFLPRETKEEVPRVAGVVLENGKEQLMFEIPLNDSGSAGLGVSLKGNKSRETREDLGIFIKSIIHGGAAYKDGRLRINDQLVAVNGESLLGRSNHAAMETLRRSMSQEGNVRGTIQLVVLRTPQDQHEASPNRSFDSSSGLFGSISPVKGMLSPITDSTQSPLVTNAIYASTVANGGYSVHMDDEDEGGPLSHGFDSQPPYLQERKSSSTSGSAQPQSPAQNQNQNQQQFHHMKASKSMDLGTSQSQRPIVEDESNVGSLSGNVSAPPTPVELGPTLGLKKSSSLESLQTAILEVNRKNELLPFHRPRPNMVRGRGCNESFRAAIDKSYDGPAEDDEDEGSDVSSGRETPASSSSLQGAGDHAEDRSKKDKKKASKNKKKEKNKPKAKEKKKAEELEETKKSKKMVFGLLRFGKKKEEKKEAKAALQRQKSDLMSDHELERLKEERERIEASHPELQQGGAGASSFPDVEDDEADPNYARIQTFRDRESSPLPQPPQAPRYGMAPPPPAQRPSPQSPPRFQGGHAEDAGAVPDDEHVDRLYAKVNKQRSAGAASPATTHSTDRIQQLRREYQQARREGAAPPYEEAEPRVRAHESSAHRIPGRGPDPRGTPRYEEVERQYASLPRRGPLDPAEYPMQPWSGLYPGPPQTQQGYPQATMYPDSNSQSVPTYSTFKPGPPYPRPGDHRPVDPGYYPNPGPQQRGPLRQDVPPSPTPPLRVPRYDTMTRGTVGGAYR

Target ORF information:

RefSeq Version	XM_020702143.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes partitioning defective 3 homolog B-like (LOC101160056), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020702143.1

ORF Insert Sequence:

ATGAAGATGA AGGTGACGGT GACGTTCGGA CAGACAGGTG TGGTGGTCCC CTGTAAGGAG 
GGCTGGACGG TGAGGGACCT GATCCAACAG GCCACGCAGA GGTACCGGAA GCTGCTGGAG 
CAGGAAGGAG ATGTTCTGGT CCGGACTCAC CATGTTGAAT ACTGCGATGG AGGGATCCTG 
GATCCTGATG ACATTCTTAG TGATCTGGTG GATGACAGGG ACAAGCTGAT GGCGGTGTAT 
GAGGAGCAGG AAGCCCAGCA GACGGCTGTG GCTAACTCGA GGTTGACCCC GAGCCCCGAC 
CTCCACCAAG CTGAGCTTTC TGTCTTCCAA CCAACCGCAA GAGGAGAGAT TGAAGTCAAC 
TCCTCGGCTC TTAAATCCGG GACCCCCCTG TTGGTGAGGA GCAGCAGCGA CTCGGCTCTC 
ACTCCACAAA CAGAGATTGA GGGCTCCTTC AGCCAAGAAA CACCTGTGAA GGCAGCCAGC 
CCACCTGTGG AGAAGCCTGG AGGGCTTGAC GGAGCCAAAC ACGCCTTCAA CGGCAGCCTG 
ACCAGATCGG TGGAGGTCCT GGGAGACGAC AGTCCTCTGG GGATCCACGT GGTTCCTTAC 
TGCTCCTCAC TCAGCGGACG GTTCCTCGGC CTTTATATCC GCGGCGTGGA GGAGAAAAGT 
CGCTCTAGAA AAGAAGGCCT ACTCCAAGAG GACGAGTGCA TCGTCAGAAT CAACAACACT 
GACCTCATGG ACAAAACCTT CAGCCAAGCT CAGGACATCT TCCGTCAGGC GATGCGCTCC 
CCAGTCATCT TTCTGGAAGT CGTGCCCCCC TCCAACAAAG ACAGATATGA AAGGAGTCTG 
ATTGGCCAGC TGTCTGGGAC CAGTTCTGGT GCCACCCACA GCGGTTCCTT CTCTGCCAAG 
GCCAAAGACC CCCCACCACC CATAAAGGCC AAGCCGGTAT TCAAGCCCCC TGAGAATCTG 
AGCCCACGAC AAACCGAAGA CGCCAGCCCT CCTGAGCCAG TGGCTACCCT GAAAGGAAGA 
AGCGAGAGCC CCCTTCTTAG GAAGAGCCCC GCCTTCACCA GCATGGTGAC CAATAAGAGA 
GGAGGACGGA AACTGAGGAT CGACTTAACA AAAGGCTCTG AGGGGCTCGG ATTCACCGTC 
GTAACCAGAG ATTCTTCAGT CCACGGTCCA GGTCCCATCC TCGTCAAGAA CATTCTGCCC 
CGAGGAGCTG CGGTCAAAGA TGGACGCCTT CAGTCCGGAG ACCGGATCCT GGAGGTGAAC 
GGCGTGGACA TCACAGGCGT GGGCCAGGAG GAGCTGGTGT GTCTGTTACG CAGCACGCGG 
CAGGGGGAGA CCGTATCGCT GGGGGTGCTG CGGCAGGAGG ACGTGTTCCT GCCCAGAGAG 
ACGAAGGAGG AGGTGCCCCG CGTGGCGGGA GTCGTCCTGG AGAACGGAAA GGAGCAGCTG 
ATGTTCGAGA TTCCCCTGAA CGACAGCGGC TCGGCCGGAC TCGGCGTCAG CCTCAAGGGG 
AACAAGTCCA GGGAGACCAG GGAAGATCTG GGAATCTTCA TCAAGTCCAT CATCCACGGA 
GGAGCTGCTT ACAAGGACGG GCGTCTGAGG ATCAACGACC AGCTCGTGGC CGTTAACGGA 
GAGTCTCTGC TGGGGCGCTC CAATCACGCC GCCATGGAGA CGCTGCGGCG GTCCATGTCC 
CAGGAGGGGA ACGTCAGAGG GACCATCCAG CTGGTGGTGC TGAGGACTCC CCAGGATCAG 
CACGAGGCGT CGCCCAATCG CTCCTTTGAC AGCTCCTCTG GTCTGTTCGG GTCCATCAGT 
CCAGTTAAAG GAATGCTCAG TCCCATCACA GACTCCACCC AGTCGCCGTT GGTGACCAAC 
GCCATCTATG CTTCCACCGT GGCCAATGGG GGCTACTCTG TGCACATGGA CGATGAGGAC 
GAGGGGGGGC CACTCAGTCA CGGGTTTGAC AGCCAGCCCC CCTACCTGCA GGAAAGGAAA 
AGTTCCTCGA CCTCCGGCTC AGCCCAACCA CAGTCACCCG CCCAGAACCA GAACCAGAAC 
CAGCAGCAGT TCCATCACAT GAAGGCCTCC AAGAGCATGG ACCTGGGTAC GAGTCAGAGC 
CAGCGGCCCA TCGTGGAGGA CGAGAGCAAC GTTGGATCTC TGAGCGGAAA CGTCTCAGCT 
CCTCCTACTC CTGTGGAACT GGGGCCTACT CTCGGTCTGA AGAAGTCCAG CTCCTTGGAG 
AGTCTGCAGA CCGCCATTCT AGAGGTCAAC AGGAAGAACG AGCTCCTCCC ATTCCACCGG 
CCGCGCCCAA ATATGGTCAG AGGACGAGGT TGCAACGAAA GCTTCAGAGC GGCTATTGAT 
AAATCCTATG ACGGCCCGGC TGAGGATGAC GAGGACGAAG GTTCAGACGT GAGCTCCGGC 
CGGGAAACGC CTGCCAGCAG CTCCTCGCTG CAGGGCGCGG GCGACCACGC CGAGGACAGG 
AGCAAAAAAG ACAAGAAGAA GGCGTCGAAA AACAAGAAGA AAGAGAAGAA CAAACCCAAA 
GCAAAGGAGA AGAAGAAGGC AGAGGAGCTC GAGGAAACCA AGAAGAGCAA GAAAATGGTC 
TTCGGCCTGT TGAGATTTGG GAAGAAAAAG GAGGAGAAGA AGGAAGCCAA GGCGGCTCTG 
CAGCGGCAAA AGTCGGACCT GATGAGCGAC CACGAGCTGG AAAGGCTGAA GGAAGAGCGA 
GAGAGAATCG AGGCGTCCCA TCCGGAGCTG CAGCAGGGGG GGGCTGGAGC TTCCTCGTTC 
CCAGACGTGG AGGACGACGA GGCCGACCCC AACTACGCCC GGATTCAGAC GTTCAGAGAC 
AGAGAGAGCA GCCCGCTGCC CCAGCCGCCT CAAGCGCCGC GCTACGGCAT GGCTCCACCC 
CCTCCAGCCC AAAGGCCCTC CCCCCAGAGC CCACCACGCT TTCAGGGAGG TCACGCTGAA 
GACGCAGGTG CCGTCCCTGA CGATGAGCAC GTGGACAGGC TGTACGCCAA AGTCAACAAG 
CAGAGGAGCG CCGGGGCGGC GTCCCCTGCC ACCACCCACA GCACCGACCG GATCCAGCAG 
CTCAGGAGAG AGTATCAGCA GGCCAGGCGG GAAGGGGCGG CGCCGCCGTA CGAGGAGGCG 
GAGCCACGAG TCCGAGCGCA TGAAAGCAGC GCTCACAGGA TTCCAGGCAG GGGGCCCGAT 
CCCCGTGGGA CCCCCCGCTA TGAGGAGGTG GAGCGTCAGT ATGCCTCCTT ACCCAGACGC 
GGCCCGTTGG ACCCTGCCGA GTACCCCATG CAGCCATGGT CAGGTCTTTA TCCTGGACCC 
CCCCAGACCC AGCAGGGATA CCCCCAGGCT ACCATGTACC CTGATTCCAA CTCCCAGTCC 
GTCCCAACGT ACTCCACCTT CAAGCCCGGC CCCCCGTACC CAAGACCCGG GGACCATCGA 
CCCGTGGACC CTGGATACTA CCCCAACCCG GGCCCCCAGC AGAGGGGCCC CCTGCGGCAG 
GACGTGCCCC CATCTCCCAC CCCTCCACTC CGTGTGCCCC GCTATGACAC CATGACACGT 
GGAACTGTGG GAGGAGCCTA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101160056 ( XM_011473669.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011471971.2 Oryzias latipes partitioning defective 3 homolog B-like (LOC101160056), transcript variant X3, mRNA.

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CloneID	OJa69324
Clone ID Related Accession (Same CDS sequence)	XM_011473669.2
Accession Version	XM_011473669.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	partitioning defective 3 homolog B-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004095217.1) and transcript sequence (GFIO01015727.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011473669.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1422 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAAGATGA AGGTGACGGT GACGTTCGGA CAGACAGGTG TGGTGGTCCC CTGTAAGGAG 
GGCTGGACGG TGAGGGACCT GATCCAACAG GCCACGCAGA GGTACCGGAA GCTGCTGGAG 
CAGGAAGGAG ATGTTCTGGT CCGGACTCAC CATGTTGAAT ACTGCGATGG AGGGATCCTG 
GATCCTGATG ACATTCTTAG TGATCTGGTG GATGACAGGG ACAAGCTGAT GGCGGTGTAT 
GAGGAGCAGG AAGCCCAGCA GACGGCTGTG GCTAACTCGA GGTTGACCCC GAGCCCCGAC 
CTCCACCAAG CTGAGCTTTC TGTCTTCCAA CCAACCGCAA GAGGAGAGAT TGAAGTCAAC 
TCCTCGGCTC TTAAATCCGG GACCCCCCTG TTGGTGAGGA GCAGCAGCGA CTCGGCTCTC 
ACTCCACAAA CAGAGATTGA GGGCTCCTTC AGCCAAGAAA CACCTGTGAA GGCAGCCAGC 
CCACCTGTGG AGAAGCCTGG AGGGCTTGAC GGAGCCAAAC ACGCCTTCAA CGGCAGCCTG 
ACCAGATCGG TGGAGGTCCT GGGAGACGAC AGTCCTCTGG GGATCCACGT GGTTCCTTAC 
TGCTCCTCAC TCAGCGGACG GTTCCTCGGC CTTTATATCC GCGGCGTGGA GGAGAAAAGT 
CGCTCTAGAA AAGAAGGCCT ACTCCAAGAG GACGAGTGCA TCGTCAGAAT CAACAACACT 
GACCTCATGG ACAAAACCTT CAGCCAAGCT CAGGACATCT TCCGTCAGGC GATGCGCTCC 
CCAGTCATCT TTCTGGAAGT CGTGCCCCCC TCCAACAAAG ACAGATATGA AAGGAGTCTG 
ATTGGCCAGC TGTCTGGGAC CAGTTCTGGT GCCACCCACA GCGGTTCCTT CTCTGCCAAG 
GCCAAAGACC CCCCACCACC CATAAAGGCC AAGCCGGTAT TCAAGCCCCC TGAGAATCTG 
AGCCCACGAC AAACCGAAGA CGCCAGCCCT CCTGAGCCAG TGGCTACCCT GAAAGGAAGA 
AGCGAGAGCC CCCTTCTTAG GAAGAGCCCC GCCTTCACCA GCATGGTGAC CAATAAGAGA 
GGAGGACGGA AACTGAGGAT CGACTTAACA AAAGGCTCTG AGGGGCTCGG ATTCACCGTC 
GTAACCAGAG ATTCTTCAGT CCACGGTCCA GGTCCCATCC TCGTCAAGAA CATTCTGCCC 
CGAGGAGCTG CGGTCAAAGA TGGACGCCTT CAGTCCGGAG ACCGGATCCT GGAGGTGAAC 
GGCGTGGACA TCACAGGCGT GGGCCAGGAG GAGCTGGTGT GTCTGTTACG CAGCACGCGG 
CAGGGGGAGA CCGTATCGCT GGGGGTGCTG CGGCAGGAGG ACGTGTTCCT GCCCAGAGAG 
ACGAAGGAGG AGGTGCCCCG CGTGGCGGGA GTCGTCCTGG AGAACGGAAA GGAGCAGCTG 
ATGTTCGAGA TTCCCCTGAA CGACAGCGGC TCGGCCGGAC TCGGCGTCAG CCTCAAGGGG 
AACAAGTCCA GGGAGACCAG GGAAGATCTG GGAATCTTCA TCAAGTCCAT CATCCACGGA 
GGAGCTGCTT ACAAGGACGG GCGTCTGAGG ATCAACGACC AGCTCGTGGC CGTTAACGGA 
GAGTCTCTGC TGGGGCGCTC CAATCACGCC GCCATGGAGA CGCTGCGGCG GTCCATGTCC 
CAGGAGGGGA ACGTCAGAGG GACCATCCAG CTGGTGGTGC TGAGGACTCC CCAGGATCAG 
CACGAGGCGT CGCCCAATCG CTCCTTTGAC AGCTCCTCTG GTCTGTTCGG GTCCATCAGT 
CCAGTTAAAG GAATGCTCAG TCCCATCACA GACTCCACCC AGTCGCCGTT GGTGACCAAC 
GCCATCTATG CTTCCACCGT GGCCAATGGG GGCTACTCTG TGCACATGGA CGATGAGGAC 
GAGGGGGGGC CACTCAGTCA CGGGTTTGAC AGCCAGCCCC CCTACCTGCA GGAAAGGAAA 
AGTTCCTCGA CCTCCGGCTC AGCCCAACCA CAGTCACCCG CCCAGAACCA GAACCAGAAC 
CAGCAGCAGT TCCATCACAT GAAGGCCTCC AAGAGCATGG ACCTGGTGGA GGACGAGAGC 
AACGTTGGAT CTCTGAGCGG AAACGTCTCA GCTCCTCCTA CTCCTGTGGA ACTGGGGCCT 
ACTCTCGGTC TGAAGAAGTC CAGCTCCTTG GAGAGTCTGC AGACCGCCAT TCTAGAGGTC 
AACAGGAAGA ACGAGCTCCT CCCATTCCAC CGGCCGCGCC CAAATATGGT CAGAGGACGA 
GGTTGCAACG AAAGCTTCAG AGCGGCTATT GATAAATCCT ATGACGGCCC GGCTGAGGAT 
GACGAGGACG AAGGTTCAGA CGTGAGCTCC GGCCGGGAAA CGCCTGCCAG CAGCTCCTCG 
CTGCAGGGCG CGGGCGACCA CGCCGAGGAC AGGAGCAAAA AAGACAAGAA GAAGGCGTCG 
AAAAACAAGA AGAAAGAGAA GAACAAACCC AAAGCAAAGG AGAAGAAGAA GGCAGAGGAG 
CTCGAGGAAA CCAAGAAGAG CAAGAAAATG GTCTTCGGCC TGTTGAGATT TGGGAAGAAA 
AAGGAGGAGA AGAAGGAAGC CAAGGCGGCT CTGCAGCGGC AAAAGTCGGA CCTGATGAGC 
GACCACGAGC TGGAAAGGCT GAAGGAAGAG CGAGAGAGAA TCGAGGCGTC CCATCCGGAG 
CTGCAGCAGG GGGGGGCTGG AGCTTCCTCG TTCCCAGACG TGGAGGACGA CGAGGCCGAC 
CCCAACTACG CCCGGATTCA GACGTTCAGA GACAGAGAGA GCAGCCCGCT GCCCCAGCCG 
CCTCAAGCGC CGCGCTACGG CATGGCTCCA CCCCCTCCAG CCCAAAGGCC CTCCCCCCAG 
AGCCCACCAC GCTTTCAGGG AGGTCACGCT GAAGACGCAG GTGCCGTCCC TGACGATGAG 
CACGTGGACA GGCTGTACGC CAAAGTCAAC AAGCAGAGGA GCGCCGGGGC GGCGTCCCCT 
GCCACCACCC ACAGCAGCAC CGACCGGATC CAGCAGCTCA GGAGAGAGTA TCAGCAGGCC 
AGGCGGGAAG GGGCGGCGCC GCCGTACGAG GAGGCGGAGC CACGAGTCCG AGCGCATGAA 
AGCAGCGCTC ACAGGATTCC AGGCAGGGGG CCCGATCCCC GTGGGACCCC CCGCTATGAG 
GAGGTGGAGC GTCAGTATGC CTCCTTACCC AGACGCGGCC CGTTGGACCC TGCCGAGTAC 
CCCATGCAGC CATGGTCAGG TCTTTATCCT GGACCCCCCC AGACCCAGCA GGGATACCCC 
CAGGCTACCA TGTACCCTGA TTCCAACTCC CAGTCCGTCC CAACGTACTC CACCTTCAAG 
CCCGGCCCCC CGTACCCAAG ACCCGGGGAC CATCGACCCG TGGACCCTGG ATACTACCCC 
AACCCGGGCC CCCAGCAGAG GGGCCCCCTG CGGCAGGACG TGCCCCCATC TCCCACCCCT 
CCACTCCGTG TGCCCCGCTA TGACACCATG ACACGTGGAA CTGTGGGAGG AGCCTACAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011471971.2
CDS	823..4365
Translation	MKMKVTVTFGQTGVVVPCKEGWTVRDLIQQATQRYRKLLEQEGDVLVRTHHVEYCDGGILDPDDILSDLVDDRDKLMAVYEEQEAQQTAVANSRLTPSPDLHQAELSVFQPTARGEIEVNSSALKSGTPLLVRSSSDSALTPQTEIEGSFSQETPVKAASPPVEKPGGLDGAKHAFNGSLTRSVEVLGDDSPLGIHVVPYCSSLSGRFLGLYIRGVEEKSRSRKEGLLQEDECIVRINNTDLMDKTFSQAQDIFRQAMRSPVIFLEVVPPSNKDRYERSLIGQLSGTSSGATHSGSFSAKAKDPPPPIKAKPVFKPPENLSPRQTEDASPPEPVATLKGRSESPLLRKSPAFTSMVTNKRGGRKLRIDLTKGSEGLGFTVVTRDSSVHGPGPILVKNILPRGAAVKDGRLQSGDRILEVNGVDITGVGQEELVCLLRSTRQGETVSLGVLRQEDVFLPRETKEEVPRVAGVVLENGKEQLMFEIPLNDSGSAGLGVSLKGNKSRETREDLGIFIKSIIHGGAAYKDGRLRINDQLVAVNGESLLGRSNHAAMETLRRSMSQEGNVRGTIQLVVLRTPQDQHEASPNRSFDSSSGLFGSISPVKGMLSPITDSTQSPLVTNAIYASTVANGGYSVHMDDEDEGGPLSHGFDSQPPYLQERKSSSTSGSAQPQSPAQNQNQNQQQFHHMKASKSMDLVEDESNVGSLSGNVSAPPTPVELGPTLGLKKSSSLESLQTAILEVNRKNELLPFHRPRPNMVRGRGCNESFRAAIDKSYDGPAEDDEDEGSDVSSGRETPASSSSLQGAGDHAEDRSKKDKKKASKNKKKEKNKPKAKEKKKAEELEETKKSKKMVFGLLRFGKKKEEKKEAKAALQRQKSDLMSDHELERLKEERERIEASHPELQQGGAGASSFPDVEDDEADPNYARIQTFRDRESSPLPQPPQAPRYGMAPPPPAQRPSPQSPPRFQGGHAEDAGAVPDDEHVDRLYAKVNKQRSAGAASPATTHSSTDRIQQLRREYQQARREGAAPPYEEAEPRVRAHESSAHRIPGRGPDPRGTPRYEEVERQYASLPRRGPLDPAEYPMQPWSGLYPGPPQTQQGYPQATMYPDSNSQSVPTYSTFKPGPPYPRPGDHRPVDPGYYPNPGPQQRGPLRQDVPPSPTPPLRVPRYDTMTRGTVGGAYR

Target ORF information:

RefSeq Version	XM_011473669.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes partitioning defective 3 homolog B-like (LOC101160056), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011473669.2

ORF Insert Sequence:

ATGAAGATGA AGGTGACGGT GACGTTCGGA CAGACAGGTG TGGTGGTCCC CTGTAAGGAG 
GGCTGGACGG TGAGGGACCT GATCCAACAG GCCACGCAGA GGTACCGGAA GCTGCTGGAG 
CAGGAAGGAG ATGTTCTGGT CCGGACTCAC CATGTTGAAT ACTGCGATGG AGGGATCCTG 
GATCCTGATG ACATTCTTAG TGATCTGGTG GATGACAGGG ACAAGCTGAT GGCGGTGTAT 
GAGGAGCAGG AAGCCCAGCA GACGGCTGTG GCTAACTCGA GGTTGACCCC GAGCCCCGAC 
CTCCACCAAG CTGAGCTTTC TGTCTTCCAA CCAACCGCAA GAGGAGAGAT TGAAGTCAAC 
TCCTCGGCTC TTAAATCCGG GACCCCCCTG TTGGTGAGGA GCAGCAGCGA CTCGGCTCTC 
ACTCCACAAA CAGAGATTGA GGGCTCCTTC AGCCAAGAAA CACCTGTGAA GGCAGCCAGC 
CCACCTGTGG AGAAGCCTGG AGGGCTTGAC GGAGCCAAAC ACGCCTTCAA CGGCAGCCTG 
ACCAGATCGG TGGAGGTCCT GGGAGACGAC AGTCCTCTGG GGATCCACGT GGTTCCTTAC 
TGCTCCTCAC TCAGCGGACG GTTCCTCGGC CTTTATATCC GCGGCGTGGA GGAGAAAAGT 
CGCTCTAGAA AAGAAGGCCT ACTCCAAGAG GACGAGTGCA TCGTCAGAAT CAACAACACT 
GACCTCATGG ACAAAACCTT CAGCCAAGCT CAGGACATCT TCCGTCAGGC GATGCGCTCC 
CCAGTCATCT TTCTGGAAGT CGTGCCCCCC TCCAACAAAG ACAGATATGA AAGGAGTCTG 
ATTGGCCAGC TGTCTGGGAC CAGTTCTGGT GCCACCCACA GCGGTTCCTT CTCTGCCAAG 
GCCAAAGACC CCCCACCACC CATAAAGGCC AAGCCGGTAT TCAAGCCCCC TGAGAATCTG 
AGCCCACGAC AAACCGAAGA CGCCAGCCCT CCTGAGCCAG TGGCTACCCT GAAAGGAAGA 
AGCGAGAGCC CCCTTCTTAG GAAGAGCCCC GCCTTCACCA GCATGGTGAC CAATAAGAGA 
GGAGGACGGA AACTGAGGAT CGACTTAACA AAAGGCTCTG AGGGGCTCGG ATTCACCGTC 
GTAACCAGAG ATTCTTCAGT CCACGGTCCA GGTCCCATCC TCGTCAAGAA CATTCTGCCC 
CGAGGAGCTG CGGTCAAAGA TGGACGCCTT CAGTCCGGAG ACCGGATCCT GGAGGTGAAC 
GGCGTGGACA TCACAGGCGT GGGCCAGGAG GAGCTGGTGT GTCTGTTACG CAGCACGCGG 
CAGGGGGAGA CCGTATCGCT GGGGGTGCTG CGGCAGGAGG ACGTGTTCCT GCCCAGAGAG 
ACGAAGGAGG AGGTGCCCCG CGTGGCGGGA GTCGTCCTGG AGAACGGAAA GGAGCAGCTG 
ATGTTCGAGA TTCCCCTGAA CGACAGCGGC TCGGCCGGAC TCGGCGTCAG CCTCAAGGGG 
AACAAGTCCA GGGAGACCAG GGAAGATCTG GGAATCTTCA TCAAGTCCAT CATCCACGGA 
GGAGCTGCTT ACAAGGACGG GCGTCTGAGG ATCAACGACC AGCTCGTGGC CGTTAACGGA 
GAGTCTCTGC TGGGGCGCTC CAATCACGCC GCCATGGAGA CGCTGCGGCG GTCCATGTCC 
CAGGAGGGGA ACGTCAGAGG GACCATCCAG CTGGTGGTGC TGAGGACTCC CCAGGATCAG 
CACGAGGCGT CGCCCAATCG CTCCTTTGAC AGCTCCTCTG GTCTGTTCGG GTCCATCAGT 
CCAGTTAAAG GAATGCTCAG TCCCATCACA GACTCCACCC AGTCGCCGTT GGTGACCAAC 
GCCATCTATG CTTCCACCGT GGCCAATGGG GGCTACTCTG TGCACATGGA CGATGAGGAC 
GAGGGGGGGC CACTCAGTCA CGGGTTTGAC AGCCAGCCCC CCTACCTGCA GGAAAGGAAA 
AGTTCCTCGA CCTCCGGCTC AGCCCAACCA CAGTCACCCG CCCAGAACCA GAACCAGAAC 
CAGCAGCAGT TCCATCACAT GAAGGCCTCC AAGAGCATGG ACCTGGTGGA GGACGAGAGC 
AACGTTGGAT CTCTGAGCGG AAACGTCTCA GCTCCTCCTA CTCCTGTGGA ACTGGGGCCT 
ACTCTCGGTC TGAAGAAGTC CAGCTCCTTG GAGAGTCTGC AGACCGCCAT TCTAGAGGTC 
AACAGGAAGA ACGAGCTCCT CCCATTCCAC CGGCCGCGCC CAAATATGGT CAGAGGACGA 
GGTTGCAACG AAAGCTTCAG AGCGGCTATT GATAAATCCT ATGACGGCCC GGCTGAGGAT 
GACGAGGACG AAGGTTCAGA CGTGAGCTCC GGCCGGGAAA CGCCTGCCAG CAGCTCCTCG 
CTGCAGGGCG CGGGCGACCA CGCCGAGGAC AGGAGCAAAA AAGACAAGAA GAAGGCGTCG 
AAAAACAAGA AGAAAGAGAA GAACAAACCC AAAGCAAAGG AGAAGAAGAA GGCAGAGGAG 
CTCGAGGAAA CCAAGAAGAG CAAGAAAATG GTCTTCGGCC TGTTGAGATT TGGGAAGAAA 
AAGGAGGAGA AGAAGGAAGC CAAGGCGGCT CTGCAGCGGC AAAAGTCGGA CCTGATGAGC 
GACCACGAGC TGGAAAGGCT GAAGGAAGAG CGAGAGAGAA TCGAGGCGTC CCATCCGGAG 
CTGCAGCAGG GGGGGGCTGG AGCTTCCTCG TTCCCAGACG TGGAGGACGA CGAGGCCGAC 
CCCAACTACG CCCGGATTCA GACGTTCAGA GACAGAGAGA GCAGCCCGCT GCCCCAGCCG 
CCTCAAGCGC CGCGCTACGG CATGGCTCCA CCCCCTCCAG CCCAAAGGCC CTCCCCCCAG 
AGCCCACCAC GCTTTCAGGG AGGTCACGCT GAAGACGCAG GTGCCGTCCC TGACGATGAG 
CACGTGGACA GGCTGTACGC CAAAGTCAAC AAGCAGAGGA GCGCCGGGGC GGCGTCCCCT 
GCCACCACCC ACAGCAGCAC CGACCGGATC CAGCAGCTCA GGAGAGAGTA TCAGCAGGCC 
AGGCGGGAAG GGGCGGCGCC GCCGTACGAG GAGGCGGAGC CACGAGTCCG AGCGCATGAA 
AGCAGCGCTC ACAGGATTCC AGGCAGGGGG CCCGATCCCC GTGGGACCCC CCGCTATGAG 
GAGGTGGAGC GTCAGTATGC CTCCTTACCC AGACGCGGCC CGTTGGACCC TGCCGAGTAC 
CCCATGCAGC CATGGTCAGG TCTTTATCCT GGACCCCCCC AGACCCAGCA GGGATACCCC 
CAGGCTACCA TGTACCCTGA TTCCAACTCC CAGTCCGTCC CAACGTACTC CACCTTCAAG 
CCCGGCCCCC CGTACCCAAG ACCCGGGGAC CATCGACCCG TGGACCCTGG ATACTACCCC 
AACCCGGGCC CCCAGCAGAG GGGCCCCCTG CGGCAGGACG TGCCCCCATC TCCCACCCCT 
CCACTCCGTG TGCCCCGCTA TGACACCATG ACACGTGGAA CTGTGGGAGG AGCCTACAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101160056 ( XM_020702144.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020557803.1 Oryzias latipes partitioning defective 3 homolog B-like (LOC101160056), transcript variant X4, mRNA.

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CloneID	OJa69325
Clone ID Related Accession (Same CDS sequence)	XM_020702144.1
Accession Version	XM_020702144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	partitioning defective 3 homolog B-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004095217.1) and transcript sequence (GFIO01015727.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1422 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTGGGCT CCTTGGACGT CCTGTACGGC GTCCTCATCC ACAAGCTGAT GGCGGTGTAT 
GAGGAGCAGG AAGCCCAGCA GACGGCTGTG GCTAACTCGA GGTTGACCCC GAGCCCCGAC 
CTCCACCAAG CTGAGCTTTC TGTCTTCCAA CCAACCGCAA GAGGAGAGAT TGAAGTCAAC 
TCCTCGGCTC TTAAATCCGG GACCCCCCTG TTGGTGAGGA GCAGCAGCGA CTCGGCTCTC 
ACTCCACAAA CAGAGATTGA GGGCTCCTTC AGCCAAGAAA CACCTGTGAA GGCAGCCAGC 
CCACCTGTGG AGAAGCCTGG AGGGCTTGAC GGAGCCAAAC ACGCCTTCAA CGGCAGCCTG 
ACCAGATCGG TGGAGGTCCT GGGAGACGAC AGTCCTCTGG GGATCCACGT GGTTCCTTAC 
TGCTCCTCAC TCAGCGGACG GTTCCTCGGC CTTTATATCC GCGGCGTGGA GGAGAAAAGT 
CGCTCTAGAA AAGAAGGCCT ACTCCAAGAG GACGAGTGCA TCGTCAGAAT CAACAACACT 
GACCTCATGG ACAAAACCTT CAGCCAAGCT CAGGACATCT TCCGTCAGGC GATGCGCTCC 
CCAGTCATCT TTCTGGAAGT CGTGCCCCCC TCCAACAAAG ACAGATATGA AAGGAGTCTG 
ATTGGCCAGC TGTCTGGGAC CAGTTCTGGT GCCACCCACA GCGGTTCCTT CTCTGCCAAG 
GCCAAAGACC CCCCACCACC CATAAAGGCC AAGCCGGTAT TCAAGCCCCC TGAGAATCTG 
AGCCCACGAC AAACCGAAGA CGCCAGCCCT CCTGAGCCAG TGGCTACCCT GAAAGGAAGA 
AGCGAGAGCC CCCTTCTTAG GAAGAGCCCC GCCTTCACCA GCATGGTGAC CAATAAGAGA 
GGAGGACGGA AACTGAGGAT CGACTTAACA AAAGGCTCTG AGGGGCTCGG ATTCACCGTC 
GTAACCAGAG ATTCTTCAGT CCACGGTCCA GGTCCCATCC TCGTCAAGAA CATTCTGCCC 
CGAGGAGCTG CGGTCAAAGA TGGACGCCTT CAGTCCGGAG ACCGGATCCT GGAGGTGAAC 
GGCGTGGACA TCACAGGCGT GGGCCAGGAG GAGCTGGTGT GTCTGTTACG CAGCACGCGG 
CAGGGGGAGA CCGTATCGCT GGGGGTGCTG CGGCAGGAGG ACGTGTTCCT GCCCAGAGAG 
ACGAAGGAGG AGGTGCCCCG CGTGGCGGGA GTCGTCCTGG AGAACGGAAA GGAGCAGCTG 
ATGTTCGAGA TTCCCCTGAA CGACAGCGGC TCGGCCGGAC TCGGCGTCAG CCTCAAGGGG 
AACAAGTCCA GGGAGACCAG GGAAGATCTG GGAATCTTCA TCAAGTCCAT CATCCACGGA 
GGAGCTGCTT ACAAGGACGG GCGTCTGAGG ATCAACGACC AGCTCGTGGC CGTTAACGGA 
GAGTCTCTGC TGGGGCGCTC CAATCACGCC GCCATGGAGA CGCTGCGGCG GTCCATGTCC 
CAGGAGGGGA ACGTCAGAGG GACCATCCAG CTGGTGGTGC TGAGGACTCC CCAGGATCAG 
CACGAGGCGT CGCCCAATCG CTCCTTTGAC AGCTCCTCTG GTCTGTTCGG GTCCATCAGT 
CCAGTTAAAG GAATGCTCAG TCCCATCACA GACTCCACCC AGTCGCCGTT GGTGACCAAC 
GCCATCTATG CTTCCACCGT GGCCAATGGG GGCTACTCTG TGCACATGGA CGATGAGGAC 
GAGGGGGGGC CACTCAGTCA CGGGTTTGAC AGCCAGCCCC CCTACCTGCA GGAAAGGAAA 
AGTTCCTCGA CCTCCGGCTC AGCCCAACCA CAGTCACCCG CCCAGAACCA GAACCAGAAC 
CAGCAGCAGT TCCATCACAT GAAGGCCTCC AAGAGCATGG ACCTGGGTAC GAGTCAGAGC 
CAGCGGCCCA TCGTGGAGGA CGAGAGCAAC GTTGGATCTC TGAGCGGAAA CGTCTCAGCT 
CCTCCTACTC CTGTGGAACT GGGGCCTACT CTCGGTCTGA AGAAGTCCAG CTCCTTGGAG 
AGTCTGCAGA CCGCCATTCT AGAGGTCAAC AGGAAGAACG AGCTCCTCCC ATTCCACCGG 
CCGCGCCCAA ATATGGTCAG AGGACGAGGT TGCAACGAAA GCTTCAGAGC GGCTATTGAT 
AAATCCTATG ACGGCCCGGC TGAGGATGAC GAGGACGAAG GTTCAGACGT GAGCTCCGGC 
CGGGAAACGC CTGCCAGCAG CTCCTCGCTG CAGGGCGCGG GCGACCACGC CGAGGACAGG 
AGCAAAAAAG ACAAGAAGAA GGCGTCGAAA AACAAGAAGA AAGAGAAGAA CAAACCCAAA 
GCAAAGGAGA AGAAGAAGGC AGAGGAGCTC GAGGAAACCA AGAAGAGCAA GAAAATGGTC 
TTCGGCCTGT TGAGATTTGG GAAGAAAAAG GAGGAGAAGA AGGAAGCCAA GGCGGCTCTG 
CAGCGGCAAA AGTCGGACCT GATGAGCGAC CACGAGCTGG AAAGGCTGAA GGAAGAGCGA 
GAGAGAATCG AGGCGTCCCA TCCGGAGCTG CAGCAGGGGG GGGCTGGAGC TTCCTCGTTC 
CCAGACGTGG AGGACGACGA GGCCGACCCC AACTACGCCC GGATTCAGAC GTTCAGAGAC 
AGAGAGAGCA GCCCGCTGCC CCAGCCGCCT CAAGCGCCGC GCTACGGCAT GGCTCCACCC 
CCTCCAGCCC AAAGGCCCTC CCCCCAGAGC CCACCACGCT TTCAGGGAGG TCACGCTGAA 
GACGCAGGTG CCGTCCCTGA CGATGAGCAC GTGGACAGGC TGTACGCCAA AGTCAACAAG 
CAGAGGAGCG CCGGGGCGGC GTCCCCTGCC ACCACCCACA GCAGCACCGA CCGGATCCAG 
CAGCTCAGGA GAGAGTATCA GCAGGCCAGG CGGGAAGGGG CGGCGCCGCC GTACGAGGAG 
GCGGAGCCAC GAGTCCGAGC GCATGAAAGC AGCGCTCACA GGATTCCAGG CAGGGGGCCC 
GATCCCCGTG GGACCCCCCG CTATGAGGAG GTGGAGCGTC AGTATGCCTC CTTACCCAGA 
CGCGGCCCGT TGGACCCTGC CGAGTACCCC ATGCAGCCAT GGTCAGGTCT TTATCCTGGA 
CCCCCCCAGA CCCAGCAGGG ATACCCCCAG GCTACCATGT ACCCTGATTC CAACTCCCAG 
TCCGTCCCAA CGTACTCCAC CTTCAAGCCC GGCCCCCCGT ACCCAAGACC CGGGGACCAT 
CGACCCGTGG ACCCTGGATA CTACCCCAAC CCGGGCCCCC AGCAGAGGGG CCCCCTGCGG 
CAGGACGTGC CCCCATCTCC CACCCCTCCA CTCCGTGTGC CCCGCTATGA CACCATGACA 
CGTGGAACTG TGGGAGGAGC CTACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020557803.1
CDS	69..3458
Translation	MLGSLDVLYGVLIHKLMAVYEEQEAQQTAVANSRLTPSPDLHQAELSVFQPTARGEIEVNSSALKSGTPLLVRSSSDSALTPQTEIEGSFSQETPVKAASPPVEKPGGLDGAKHAFNGSLTRSVEVLGDDSPLGIHVVPYCSSLSGRFLGLYIRGVEEKSRSRKEGLLQEDECIVRINNTDLMDKTFSQAQDIFRQAMRSPVIFLEVVPPSNKDRYERSLIGQLSGTSSGATHSGSFSAKAKDPPPPIKAKPVFKPPENLSPRQTEDASPPEPVATLKGRSESPLLRKSPAFTSMVTNKRGGRKLRIDLTKGSEGLGFTVVTRDSSVHGPGPILVKNILPRGAAVKDGRLQSGDRILEVNGVDITGVGQEELVCLLRSTRQGETVSLGVLRQEDVFLPRETKEEVPRVAGVVLENGKEQLMFEIPLNDSGSAGLGVSLKGNKSRETREDLGIFIKSIIHGGAAYKDGRLRINDQLVAVNGESLLGRSNHAAMETLRRSMSQEGNVRGTIQLVVLRTPQDQHEASPNRSFDSSSGLFGSISPVKGMLSPITDSTQSPLVTNAIYASTVANGGYSVHMDDEDEGGPLSHGFDSQPPYLQERKSSSTSGSAQPQSPAQNQNQNQQQFHHMKASKSMDLGTSQSQRPIVEDESNVGSLSGNVSAPPTPVELGPTLGLKKSSSLESLQTAILEVNRKNELLPFHRPRPNMVRGRGCNESFRAAIDKSYDGPAEDDEDEGSDVSSGRETPASSSSLQGAGDHAEDRSKKDKKKASKNKKKEKNKPKAKEKKKAEELEETKKSKKMVFGLLRFGKKKEEKKEAKAALQRQKSDLMSDHELERLKEERERIEASHPELQQGGAGASSFPDVEDDEADPNYARIQTFRDRESSPLPQPPQAPRYGMAPPPPAQRPSPQSPPRFQGGHAEDAGAVPDDEHVDRLYAKVNKQRSAGAASPATTHSSTDRIQQLRREYQQARREGAAPPYEEAEPRVRAHESSAHRIPGRGPDPRGTPRYEEVERQYASLPRRGPLDPAEYPMQPWSGLYPGPPQTQQGYPQATMYPDSNSQSVPTYSTFKPGPPYPRPGDHRPVDPGYYPNPGPQQRGPLRQDVPPSPTPPLRVPRYDTMTRGTVGGAYR

Target ORF information:

RefSeq Version	XM_020702144.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes partitioning defective 3 homolog B-like (LOC101160056), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020702144.1

ORF Insert Sequence:

ATGCTGGGCT CCTTGGACGT CCTGTACGGC GTCCTCATCC ACAAGCTGAT GGCGGTGTAT 
GAGGAGCAGG AAGCCCAGCA GACGGCTGTG GCTAACTCGA GGTTGACCCC GAGCCCCGAC 
CTCCACCAAG CTGAGCTTTC TGTCTTCCAA CCAACCGCAA GAGGAGAGAT TGAAGTCAAC 
TCCTCGGCTC TTAAATCCGG GACCCCCCTG TTGGTGAGGA GCAGCAGCGA CTCGGCTCTC 
ACTCCACAAA CAGAGATTGA GGGCTCCTTC AGCCAAGAAA CACCTGTGAA GGCAGCCAGC 
CCACCTGTGG AGAAGCCTGG AGGGCTTGAC GGAGCCAAAC ACGCCTTCAA CGGCAGCCTG 
ACCAGATCGG TGGAGGTCCT GGGAGACGAC AGTCCTCTGG GGATCCACGT GGTTCCTTAC 
TGCTCCTCAC TCAGCGGACG GTTCCTCGGC CTTTATATCC GCGGCGTGGA GGAGAAAAGT 
CGCTCTAGAA AAGAAGGCCT ACTCCAAGAG GACGAGTGCA TCGTCAGAAT CAACAACACT 
GACCTCATGG ACAAAACCTT CAGCCAAGCT CAGGACATCT TCCGTCAGGC GATGCGCTCC 
CCAGTCATCT TTCTGGAAGT CGTGCCCCCC TCCAACAAAG ACAGATATGA AAGGAGTCTG 
ATTGGCCAGC TGTCTGGGAC CAGTTCTGGT GCCACCCACA GCGGTTCCTT CTCTGCCAAG 
GCCAAAGACC CCCCACCACC CATAAAGGCC AAGCCGGTAT TCAAGCCCCC TGAGAATCTG 
AGCCCACGAC AAACCGAAGA CGCCAGCCCT CCTGAGCCAG TGGCTACCCT GAAAGGAAGA 
AGCGAGAGCC CCCTTCTTAG GAAGAGCCCC GCCTTCACCA GCATGGTGAC CAATAAGAGA 
GGAGGACGGA AACTGAGGAT CGACTTAACA AAAGGCTCTG AGGGGCTCGG ATTCACCGTC 
GTAACCAGAG ATTCTTCAGT CCACGGTCCA GGTCCCATCC TCGTCAAGAA CATTCTGCCC 
CGAGGAGCTG CGGTCAAAGA TGGACGCCTT CAGTCCGGAG ACCGGATCCT GGAGGTGAAC 
GGCGTGGACA TCACAGGCGT GGGCCAGGAG GAGCTGGTGT GTCTGTTACG CAGCACGCGG 
CAGGGGGAGA CCGTATCGCT GGGGGTGCTG CGGCAGGAGG ACGTGTTCCT GCCCAGAGAG 
ACGAAGGAGG AGGTGCCCCG CGTGGCGGGA GTCGTCCTGG AGAACGGAAA GGAGCAGCTG 
ATGTTCGAGA TTCCCCTGAA CGACAGCGGC TCGGCCGGAC TCGGCGTCAG CCTCAAGGGG 
AACAAGTCCA GGGAGACCAG GGAAGATCTG GGAATCTTCA TCAAGTCCAT CATCCACGGA 
GGAGCTGCTT ACAAGGACGG GCGTCTGAGG ATCAACGACC AGCTCGTGGC CGTTAACGGA 
GAGTCTCTGC TGGGGCGCTC CAATCACGCC GCCATGGAGA CGCTGCGGCG GTCCATGTCC 
CAGGAGGGGA ACGTCAGAGG GACCATCCAG CTGGTGGTGC TGAGGACTCC CCAGGATCAG 
CACGAGGCGT CGCCCAATCG CTCCTTTGAC AGCTCCTCTG GTCTGTTCGG GTCCATCAGT 
CCAGTTAAAG GAATGCTCAG TCCCATCACA GACTCCACCC AGTCGCCGTT GGTGACCAAC 
GCCATCTATG CTTCCACCGT GGCCAATGGG GGCTACTCTG TGCACATGGA CGATGAGGAC 
GAGGGGGGGC CACTCAGTCA CGGGTTTGAC AGCCAGCCCC CCTACCTGCA GGAAAGGAAA 
AGTTCCTCGA CCTCCGGCTC AGCCCAACCA CAGTCACCCG CCCAGAACCA GAACCAGAAC 
CAGCAGCAGT TCCATCACAT GAAGGCCTCC AAGAGCATGG ACCTGGGTAC GAGTCAGAGC 
CAGCGGCCCA TCGTGGAGGA CGAGAGCAAC GTTGGATCTC TGAGCGGAAA CGTCTCAGCT 
CCTCCTACTC CTGTGGAACT GGGGCCTACT CTCGGTCTGA AGAAGTCCAG CTCCTTGGAG 
AGTCTGCAGA CCGCCATTCT AGAGGTCAAC AGGAAGAACG AGCTCCTCCC ATTCCACCGG 
CCGCGCCCAA ATATGGTCAG AGGACGAGGT TGCAACGAAA GCTTCAGAGC GGCTATTGAT 
AAATCCTATG ACGGCCCGGC TGAGGATGAC GAGGACGAAG GTTCAGACGT GAGCTCCGGC 
CGGGAAACGC CTGCCAGCAG CTCCTCGCTG CAGGGCGCGG GCGACCACGC CGAGGACAGG 
AGCAAAAAAG ACAAGAAGAA GGCGTCGAAA AACAAGAAGA AAGAGAAGAA CAAACCCAAA 
GCAAAGGAGA AGAAGAAGGC AGAGGAGCTC GAGGAAACCA AGAAGAGCAA GAAAATGGTC 
TTCGGCCTGT TGAGATTTGG GAAGAAAAAG GAGGAGAAGA AGGAAGCCAA GGCGGCTCTG 
CAGCGGCAAA AGTCGGACCT GATGAGCGAC CACGAGCTGG AAAGGCTGAA GGAAGAGCGA 
GAGAGAATCG AGGCGTCCCA TCCGGAGCTG CAGCAGGGGG GGGCTGGAGC TTCCTCGTTC 
CCAGACGTGG AGGACGACGA GGCCGACCCC AACTACGCCC GGATTCAGAC GTTCAGAGAC 
AGAGAGAGCA GCCCGCTGCC CCAGCCGCCT CAAGCGCCGC GCTACGGCAT GGCTCCACCC 
CCTCCAGCCC AAAGGCCCTC CCCCCAGAGC CCACCACGCT TTCAGGGAGG TCACGCTGAA 
GACGCAGGTG CCGTCCCTGA CGATGAGCAC GTGGACAGGC TGTACGCCAA AGTCAACAAG 
CAGAGGAGCG CCGGGGCGGC GTCCCCTGCC ACCACCCACA GCAGCACCGA CCGGATCCAG 
CAGCTCAGGA GAGAGTATCA GCAGGCCAGG CGGGAAGGGG CGGCGCCGCC GTACGAGGAG 
GCGGAGCCAC GAGTCCGAGC GCATGAAAGC AGCGCTCACA GGATTCCAGG CAGGGGGCCC 
GATCCCCGTG GGACCCCCCG CTATGAGGAG GTGGAGCGTC AGTATGCCTC CTTACCCAGA 
CGCGGCCCGT TGGACCCTGC CGAGTACCCC ATGCAGCCAT GGTCAGGTCT TTATCCTGGA 
CCCCCCCAGA CCCAGCAGGG ATACCCCCAG GCTACCATGT ACCCTGATTC CAACTCCCAG 
TCCGTCCCAA CGTACTCCAC CTTCAAGCCC GGCCCCCCGT ACCCAAGACC CGGGGACCAT 
CGACCCGTGG ACCCTGGATA CTACCCCAAC CCGGGCCCCC AGCAGAGGGG CCCCCTGCGG 
CAGGACGTGC CCCCATCTCC CACCCCTCCA CTCCGTGTGC CCCGCTATGA CACCATGACA 
CGTGGAACTG TGGGAGGAGC CTACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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LOC101160056 cDNA ORF clone, Oryzias latipes(Japanese medaka)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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