megf10 cDNA ORF clone, Oryzias latipes(Japanese medaka)

The following megf10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the megf10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OJa21746 XM_011482096.3
Latest version!
Oryzias latipes multiple EGF like domains 10 (megf10), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
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OJa21746 XM_011482096.2 Oryzias latipes multiple EGF like domains 10 (megf10), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OJa21746
    Clone ID Related Accession (Same CDS sequence) XM_011482096.3 , XM_011482096.2
    Accession Version XM_011482096.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3369bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1518537600000
    Organism Oryzias latipes(Japanese medaka)
    Product multiple epidermal growth factor-like domains protein 10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019870.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_011482096.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCTCTGT TGCTCCACGG GACACTCCTT TGCACAGCGG TCCTCTGCTT CCTTCCATCC 
    AGCACCTCCT CCCTCAGTGA TGAAGATCCC AACGTCTGCA GCCACTGGGA GAGTTACTCC
    ATGACGGTGC AGGAGACCTA CTCCCATCCT TACGATCAGG TTTTCTACAC CAGCTGCTCA
    GACATCCTGA GCTGGTTCAA ATGCACCACT CACAAAACCA GCTATCGGAT ATCATATCGC
    AGCAGAGAGA AGATCATGTA CCGCAGGAAA TCCCAGTGCT GCCCAGGCTT CTATGAAAAT
    AAAGATGTGT GCACTCCTCG TTGCACAGAG AGCTGTGTTC ACGGCCGCTG CGTGGCCCCC
    AACAGCTGCC AGTGTGAGCC AGGCTGGGGG GGCTCCAACT GCTCCAGCGC CTGTGATAGC
    AGTCACTGGG GTCCACACTG CAGCAACAGA TGTCAGTGCC AGCATGGAGC GCTTTGCAAC
    CCCATTACTG GAGCTTGTGT CTGCAGCCCG GGCTACAGAG GCTGGCGCTG CGAGTCCCAC
    TGTGGAACCG GCACCTACGG CAATGGCTGC CAGCAGAAGT GTCAGTGCCA GAACGGAGCA
    TCCTGTCACC ATGTGACCGG AGAGTGCACG TGCTCAGCCG GATACACCGG AGCTTTCTGT
    GAGGACCTGT GTCCTCCTGG TAAACATGGA CAGCAGTGCG AGGAGAGATG TCCCTGTCAG
    AATGGGGGAG TGTGTCATCA CGTGACTGGA GACTGCTCCT GTCCTGCCGG ATGGAGGGGG
    ACGGTGTGTG GTCAACCGTG TCCAGAGGGA CGGTTTGGTC TGGACTGCTC CCAGGAATGC
    CAGTGCCACA ATGATGCCGT TTGTTTGTCG ACATCCGGGG AGTGTCTCTG CCGTGAGGGA
    TACACCGGAG AGCGGTGTCA GGACCAGTGC CCTGTGGGGA CCTACGGTGC CGGCTGCACA
    AAATCATGTA GCTGTGAGAA CAACAGCAAG TGCGACCACA AGACTGGATC ATGTCTTTGT
    GAGCCGGGCT TCATGGGAGA ATCCTGCCAC GTCCGTTTTT GTCCCGAAGG CCACTTTGGC
    CTGCACTGTG GCAGGAAGTG TCCGTGCCAC CTCCCGAACA CTCGCAGCTG CCACCCGATG
    ACCGGGGAGT GCACGTGCAG ACCCGGTTGG TCGGGTCTGT ACTGCAATGA GACATGTTCT
    CTGGGATTCT ACGGCGAGTC GTGCAAAGAA GTGTGCCAGT GCCAGAACGG AGCCGACTGC
    CACAGCGTGA CCGGGGAGTG TACCTGTGCT CCAGGCTTTA CGGGTCACAG GTGTTCCACT
    CCATGCCCGG CGGGCTCCTA TGGACTGAGC TGCTCATCCA GATGCTTTTG TAAGAACGGA
    GCTCGCTGCT CACCTGTGGA TGGATCCTGC TCCTGTGCAC CAGGCTGGCA TGGGGTGGAC
    TGCTCCATCA GCTGCCCCAG CGGGACATGG GGGCTGGGCT GCAACCTCAC CTGTGCATGT
    GCCAACGGGG GCTCCTGCAG CGCGTTGGAC GGCCGCTGCA CCTGTGCTGC TGGCTGGAGA
    GGAGAGAGGT GTGACCTGCG CTGTCAGGAC GGCACCTATG GTCTGGACTG CAGGGGGCGC
    TGCGACTGCA GCCATTCTGA TGGGTGTCAC CATTCAACCG GCCACTGCCG CTGTCTGCCC
    GGATGGACCG GGATCCACTG TGACAGCGTG TGCGCCGAGG GCCGCTGGGG GCCCAACTGC
    TCCACCCCCT GCAATTGTAA GAACGGAGCA TCCTGCTCTC CAGATGAAGG AACCTGCGAG
    TGTGCTCCAG GATTCAGAGG CACCACCTGT CAGAGGATCT GCTCTCCAGG TTACTTTGGT
    CACCGCTGCA GTCAGACCTG TCCTCAGTGC GTCCACAGTA ACGGGCCGTG CCACCACGTC
    AGTGGACACT GTGACTGTCT GCCTGGTTTC AGGGGGGCGC TGTGCAATGA GGTGTGTCCC
    AGTGGCCACT TTGGGAAAAA CTGCGCCTCG ACCTGCCACT GCACCAACAA CGGCACCTGC
    AACCCCATCG ACGGCTCCTG CCAGTGCTAC CCAGGGTGGA TTGGCAGCGA CTGTTCCCAG
    TCCTGTCCAC CCGGATCCTG GGGACCCAAC TGCATCCACA CCTGCACCTG CCACAACGCC
    GCGGGCTGCA GTGCCTATGA TGGAGAGTGC AAGTGCACCC CTGGGTGGAC GGGTCTCTAC
    TGCACTCAGC GCTGTCCTTT GGGTTTCTAT GGGAAGGAAT GCTCTCAGAG CTGCAGGTGC
    AGAAACGGTG CAGACTGTGA CCACATATCT GGACAGTGCA CGTGTCGCAT CGGCTTCATG
    GGACTACACT GTGAACAAAA GTGTCCTCCT GGCTCCTATG GTTATGGCTG CCGCCATGTC
    TGTGACTGCC TCAATAACTC CACGTGTGAC CACATGAGTG GAACGTGCTA CTGCAGTCCC
    GGCTGGAGAG GCGCTCGATG CGACCAAGCC GCTGCAGTGG TGGTGGGCGA CCTCAACAGT
    TTGACCAGTG TAGCCATGCA CATGGACACC TACCAGATAG GAGTCATCAC AGGAATCATT
    GTCCTGGTTC TGCTGGTGCT CCTCCTCCTG CTCCTCTTCA TCATGTATCG AAAGAAGCAG
    AAGAGCAAAG AGCCCACCAT GCCGCCTGTC AGCTACACAT CAGCAGCCCG GGTCCCACCT
    GATCATCTAG TGGCAGACAC CCCGACCAGC GAGGTCCATC CGAGTTACTT CTCCAACCCC
    AGTTATCACA CGCTGACTCA GTGCATGGGC CCGCCGTACG TCACCGGTGC CTCGTATGGG
    AAGACCAAAT TTGGCCAGCC ATCTGCTGGC ACAAAGAGCC TGGACCAGAG AAAACCAGTC
    CAGTTTGTGG CTCCCACTGG CCCTCTGAGT GCTGAATGGA ATCAAGGGGA CTACATGGAT
    GACCTGGGAG CTTATGGAGT GCAAAGAGGA CACCCTGGAA AGTCTTTAAG AGACCTGGCG
    AAAGGTGCCG CCTATCATGC CAGCACATGC TCCCTAAGCA GCTCGGAAAA TCCATATGCC
    ACAATCAAGG ACCCCCCTCT GCTGACAGCC AGAGGGACAG AATGTGGCTA TGTGGAGATG
    AAATCTCCAG CCAGAAGGGA TTCAGCGTAT GCTGAAATAA GCAACAGCAG CTTGTCCAAC
    AATCGCAATG TCTATGAAGT TGAGCCAACA ACCAGCACCA GCCAGTCCTG TGGAGACAGT
    AGCAGCGTCC TGCAGTTTGG ACAGGAGCCA TATGATCTAC CAAAACATAG CCACATCCCC
    TGTCACTATG ACCTCCTTCC TGCCAGAGAT AGTCCCTCCT CCGAGCTGCA GGAGCCAGGC
    AGTGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011480398.1
    CDS155..3523
    Translation

    Target ORF information:

    RefSeq Version XM_011482096.3
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes multiple EGF like domains 10 (megf10), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011482096.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCTCTGT TGCTCCACGG GACACTCCTT TGCACAGCGG TCCTCTGCTT CCTTCCATCC 
    AGCACCTCCT CCCTCAGTGA TGAAGATCCC AACGTCTGCA GCCACTGGGA GAGTTACTCC
    ATGACGGTGC AGGAGACCTA CTCCCATCCT TACGATCAGG TTTTCTACAC CAGCTGCTCA
    GACATCCTGA GCTGGTTCAA ATGCACCACT CACAAAACCA GCTATCGGAT ATCATATCGC
    AGCAGAGAGA AGATCATGTA CCGCAGGAAA TCCCAGTGCT GCCCAGGCTT CTATGAAAAT
    AAAGATGTGT GCACTCCTCG TTGCACAGAG AGCTGTGTTC ACGGCCGCTG CGTGGCCCCC
    AACAGCTGCC AGTGTGAGCC AGGCTGGGGG GGCTCCAACT GCTCCAGCGC CTGTGATAGC
    AGTCACTGGG GTCCACACTG CAGCAACAGA TGTCAGTGCC AGCATGGAGC GCTTTGCAAC
    CCCATTACTG GAGCTTGTGT CTGCAGCCCG GGCTACAGAG GCTGGCGCTG CGAGTCCCAC
    TGTGGAACCG GCACCTACGG CAATGGCTGC CAGCAGAAGT GTCAGTGCCA GAACGGAGCA
    TCCTGTCACC ATGTGACCGG AGAGTGCACG TGCTCAGCCG GATACACCGG AGCTTTCTGT
    GAGGACCTGT GTCCTCCTGG TAAACATGGA CAGCAGTGCG AGGAGAGATG TCCCTGTCAG
    AATGGGGGAG TGTGTCATCA CGTGACTGGA GACTGCTCCT GTCCTGCCGG ATGGAGGGGG
    ACGGTGTGTG GTCAACCGTG TCCAGAGGGA CGGTTTGGTC TGGACTGCTC CCAGGAATGC
    CAGTGCCACA ATGATGCCGT TTGTTTGTCG ACATCCGGGG AGTGTCTCTG CCGTGAGGGA
    TACACCGGAG AGCGGTGTCA GGACCAGTGC CCTGTGGGGA CCTACGGTGC CGGCTGCACA
    AAATCATGTA GCTGTGAGAA CAACAGCAAG TGCGACCACA AGACTGGATC ATGTCTTTGT
    GAGCCGGGCT TCATGGGAGA ATCCTGCCAC GTCCGTTTTT GTCCCGAAGG CCACTTTGGC
    CTGCACTGTG GCAGGAAGTG TCCGTGCCAC CTCCCGAACA CTCGCAGCTG CCACCCGATG
    ACCGGGGAGT GCACGTGCAG ACCCGGTTGG TCGGGTCTGT ACTGCAATGA GACATGTTCT
    CTGGGATTCT ACGGCGAGTC GTGCAAAGAA GTGTGCCAGT GCCAGAACGG AGCCGACTGC
    CACAGCGTGA CCGGGGAGTG TACCTGTGCT CCAGGCTTTA CGGGTCACAG GTGTTCCACT
    CCATGCCCGG CGGGCTCCTA TGGACTGAGC TGCTCATCCA GATGCTTTTG TAAGAACGGA
    GCTCGCTGCT CACCTGTGGA TGGATCCTGC TCCTGTGCAC CAGGCTGGCA TGGGGTGGAC
    TGCTCCATCA GCTGCCCCAG CGGGACATGG GGGCTGGGCT GCAACCTCAC CTGTGCATGT
    GCCAACGGGG GCTCCTGCAG CGCGTTGGAC GGCCGCTGCA CCTGTGCTGC TGGCTGGAGA
    GGAGAGAGGT GTGACCTGCG CTGTCAGGAC GGCACCTATG GTCTGGACTG CAGGGGGCGC
    TGCGACTGCA GCCATTCTGA TGGGTGTCAC CATTCAACCG GCCACTGCCG CTGTCTGCCC
    GGATGGACCG GGATCCACTG TGACAGCGTG TGCGCCGAGG GCCGCTGGGG GCCCAACTGC
    TCCACCCCCT GCAATTGTAA GAACGGAGCA TCCTGCTCTC CAGATGAAGG AACCTGCGAG
    TGTGCTCCAG GATTCAGAGG CACCACCTGT CAGAGGATCT GCTCTCCAGG TTACTTTGGT
    CACCGCTGCA GTCAGACCTG TCCTCAGTGC GTCCACAGTA ACGGGCCGTG CCACCACGTC
    AGTGGACACT GTGACTGTCT GCCTGGTTTC AGGGGGGCGC TGTGCAATGA GGTGTGTCCC
    AGTGGCCACT TTGGGAAAAA CTGCGCCTCG ACCTGCCACT GCACCAACAA CGGCACCTGC
    AACCCCATCG ACGGCTCCTG CCAGTGCTAC CCAGGGTGGA TTGGCAGCGA CTGTTCCCAG
    TCCTGTCCAC CCGGATCCTG GGGACCCAAC TGCATCCACA CCTGCACCTG CCACAACGCC
    GCGGGCTGCA GTGCCTATGA TGGAGAGTGC AAGTGCACCC CTGGGTGGAC GGGTCTCTAC
    TGCACTCAGC GCTGTCCTTT GGGTTTCTAT GGGAAGGAAT GCTCTCAGAG CTGCAGGTGC
    AGAAACGGTG CAGACTGTGA CCACATATCT GGACAGTGCA CGTGTCGCAT CGGCTTCATG
    GGACTACACT GTGAACAAAA GTGTCCTCCT GGCTCCTATG GTTATGGCTG CCGCCATGTC
    TGTGACTGCC TCAATAACTC CACGTGTGAC CACATGAGTG GAACGTGCTA CTGCAGTCCC
    GGCTGGAGAG GCGCTCGATG CGACCAAGCC GCTGCAGTGG TGGTGGGCGA CCTCAACAGT
    TTGACCAGTG TAGCCATGCA CATGGACACC TACCAGATAG GAGTCATCAC AGGAATCATT
    GTCCTGGTTC TGCTGGTGCT CCTCCTCCTG CTCCTCTTCA TCATGTATCG AAAGAAGCAG
    AAGAGCAAAG AGCCCACCAT GCCGCCTGTC AGCTACACAT CAGCAGCCCG GGTCCCACCT
    GATCATCTAG TGGCAGACAC CCCGACCAGC GAGGTCCATC CGAGTTACTT CTCCAACCCC
    AGTTATCACA CGCTGACTCA GTGCATGGGC CCGCCGTACG TCACCGGTGC CTCGTATGGG
    AAGACCAAAT TTGGCCAGCC ATCTGCTGGC ACAAAGAGCC TGGACCAGAG AAAACCAGTC
    CAGTTTGTGG CTCCCACTGG CCCTCTGAGT GCTGAATGGA ATCAAGGGGA CTACATGGAT
    GACCTGGGAG CTTATGGAGT GCAAAGAGGA CACCCTGGAA AGTCTTTAAG AGACCTGGCG
    AAAGGTGCCG CCTATCATGC CAGCACATGC TCCCTAAGCA GCTCGGAAAA TCCATATGCC
    ACAATCAAGG ACCCCCCTCT GCTGACAGCC AGAGGGACAG AATGTGGCTA TGTGGAGATG
    AAATCTCCAG CCAGAAGGGA TTCAGCGTAT GCTGAAATAA GCAACAGCAG CTTGTCCAAC
    AATCGCAATG TCTATGAAGT TGAGCCAACA ACCAGCACCA GCCAGTCCTG TGGAGACAGT
    AGCAGCGTCC TGCAGTTTGG ACAGGAGCCA TATGATCTAC CAAAACATAG CCACATCCCC
    TGTCACTATG ACCTCCTTCC TGCCAGAGAT AGTCCCTCCT CCGAGCTGCA GGAGCCAGGC
    AGTGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OJa21746
    Clone ID Related Accession (Same CDS sequence) XM_011482096.3 , XM_011482096.2
    Accession Version XM_011482096.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3369bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1491408000000
    Organism Oryzias latipes(Japanese medaka)
    Product multiple epidermal growth factor-like domains protein 10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019870.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011482096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCTCTGT TGCTCCACGG GACACTCCTT TGCACAGCGG TCCTCTGCTT CCTTCCATCC 
    AGCACCTCCT CCCTCAGTGA TGAAGATCCC AACGTCTGCA GCCACTGGGA GAGTTACTCC
    ATGACGGTGC AGGAGACCTA CTCCCATCCT TACGATCAGG TTTTCTACAC CAGCTGCTCA
    GACATCCTGA GCTGGTTCAA ATGCACCACT CACAAAACCA GCTATCGGAT ATCATATCGC
    AGCAGAGAGA AGATCATGTA CCGCAGGAAA TCCCAGTGCT GCCCAGGCTT CTATGAAAAT
    AAAGATGTGT GCACTCCTCG TTGCACAGAG AGCTGTGTTC ACGGCCGCTG CGTGGCCCCC
    AACAGCTGCC AGTGTGAGCC AGGCTGGGGG GGCTCCAACT GCTCCAGCGC CTGTGATAGC
    AGTCACTGGG GTCCACACTG CAGCAACAGA TGTCAGTGCC AGCATGGAGC GCTTTGCAAC
    CCCATTACTG GAGCTTGTGT CTGCAGCCCG GGCTACAGAG GCTGGCGCTG CGAGTCCCAC
    TGTGGAACCG GCACCTACGG CAATGGCTGC CAGCAGAAGT GTCAGTGCCA GAACGGAGCA
    TCCTGTCACC ATGTGACCGG AGAGTGCACG TGCTCAGCCG GATACACCGG AGCTTTCTGT
    GAGGACCTGT GTCCTCCTGG TAAACATGGA CAGCAGTGCG AGGAGAGATG TCCCTGTCAG
    AATGGGGGAG TGTGTCATCA CGTGACTGGA GACTGCTCCT GTCCTGCCGG ATGGAGGGGG
    ACGGTGTGTG GTCAACCGTG TCCAGAGGGA CGGTTTGGTC TGGACTGCTC CCAGGAATGC
    CAGTGCCACA ATGATGCCGT TTGTTTGTCG ACATCCGGGG AGTGTCTCTG CCGTGAGGGA
    TACACCGGAG AGCGGTGTCA GGACCAGTGC CCTGTGGGGA CCTACGGTGC CGGCTGCACA
    AAATCATGTA GCTGTGAGAA CAACAGCAAG TGCGACCACA AGACTGGATC ATGTCTTTGT
    GAGCCGGGCT TCATGGGAGA ATCCTGCCAC GTCCGTTTTT GTCCCGAAGG CCACTTTGGC
    CTGCACTGTG GCAGGAAGTG TCCGTGCCAC CTCCCGAACA CTCGCAGCTG CCACCCGATG
    ACCGGGGAGT GCACGTGCAG ACCCGGTTGG TCGGGTCTGT ACTGCAATGA GACATGTTCT
    CTGGGATTCT ACGGCGAGTC GTGCAAAGAA GTGTGCCAGT GCCAGAACGG AGCCGACTGC
    CACAGCGTGA CCGGGGAGTG TACCTGTGCT CCAGGCTTTA CGGGTCACAG GTGTTCCACT
    CCATGCCCGG CGGGCTCCTA TGGACTGAGC TGCTCATCCA GATGCTTTTG TAAGAACGGA
    GCTCGCTGCT CACCTGTGGA TGGATCCTGC TCCTGTGCAC CAGGCTGGCA TGGGGTGGAC
    TGCTCCATCA GCTGCCCCAG CGGGACATGG GGGCTGGGCT GCAACCTCAC CTGTGCATGT
    GCCAACGGGG GCTCCTGCAG CGCGTTGGAC GGCCGCTGCA CCTGTGCTGC TGGCTGGAGA
    GGAGAGAGGT GTGACCTGCG CTGTCAGGAC GGCACCTATG GTCTGGACTG CAGGGGGCGC
    TGCGACTGCA GCCATTCTGA TGGGTGTCAC CATTCAACCG GCCACTGCCG CTGTCTGCCC
    GGATGGACCG GGATCCACTG TGACAGCGTG TGCGCCGAGG GCCGCTGGGG GCCCAACTGC
    TCCACCCCCT GCAATTGTAA GAACGGAGCA TCCTGCTCTC CAGATGAAGG AACCTGCGAG
    TGTGCTCCAG GATTCAGAGG CACCACCTGT CAGAGGATCT GCTCTCCAGG TTACTTTGGT
    CACCGCTGCA GTCAGACCTG TCCTCAGTGC GTCCACAGTA ACGGGCCGTG CCACCACGTC
    AGTGGACACT GTGACTGTCT GCCTGGTTTC AGGGGGGCGC TGTGCAATGA GGTGTGTCCC
    AGTGGCCACT TTGGGAAAAA CTGCGCCTCG ACCTGCCACT GCACCAACAA CGGCACCTGC
    AACCCCATCG ACGGCTCCTG CCAGTGCTAC CCAGGGTGGA TTGGCAGCGA CTGTTCCCAG
    TCCTGTCCAC CCGGATCCTG GGGACCCAAC TGCATCCACA CCTGCACCTG CCACAACGCC
    GCGGGCTGCA GTGCCTATGA TGGAGAGTGC AAGTGCACCC CTGGGTGGAC GGGTCTCTAC
    TGCACTCAGC GCTGTCCTTT GGGTTTCTAT GGGAAGGAAT GCTCTCAGAG CTGCAGGTGC
    AGAAACGGTG CAGACTGTGA CCACATATCT GGACAGTGCA CGTGTCGCAT CGGCTTCATG
    GGACTACACT GTGAACAAAA GTGTCCTCCT GGCTCCTATG GTTATGGCTG CCGCCATGTC
    TGTGACTGCC TCAATAACTC CACGTGTGAC CACATGAGTG GAACGTGCTA CTGCAGTCCC
    GGCTGGAGAG GCGCTCGATG CGACCAAGCC GCTGCAGTGG TGGTGGGCGA CCTCAACAGT
    TTGACCAGTG TAGCCATGCA CATGGACACC TACCAGATAG GAGTCATCAC AGGAATCATT
    GTCCTGGTTC TGCTGGTGCT CCTCCTCCTG CTCCTCTTCA TCATGTATCG AAAGAAGCAG
    AAGAGCAAAG AGCCCACCAT GCCGCCTGTC AGCTACACAT CAGCAGCCCG GGTCCCACCT
    GATCATCTAG TGGCAGACAC CCCGACCAGC GAGGTCCATC CGAGTTACTT CTCCAACCCC
    AGTTATCACA CGCTGACTCA GTGCATGGGC CCGCCGTACG TCACCGGTGC CTCGTATGGG
    AAGACCAAAT TTGGCCAGCC ATCTGCTGGC ACAAAGAGCC TGGACCAGAG AAAACCAGTC
    CAGTTTGTGG CTCCCACTGG CCCTCTGAGT GCTGAATGGA ATCAAGGGGA CTACATGGAT
    GACCTGGGAG CTTATGGAGT GCAAAGAGGA CACCCTGGAA AGTCTTTAAG AGACCTGGCG
    AAAGGTGCCG CCTATCATGC CAGCACATGC TCCCTAAGCA GCTCGGAAAA TCCATATGCC
    ACAATCAAGG ACCCCCCTCT GCTGACAGCC AGAGGGACAG AATGTGGCTA TGTGGAGATG
    AAATCTCCAG CCAGAAGGGA TTCAGCGTAT GCTGAAATAA GCAACAGCAG CTTGTCCAAC
    AATCGCAATG TCTATGAAGT TGAGCCAACA ACCAGCACCA GCCAGTCCTG TGGAGACAGT
    AGCAGCGTCC TGCAGTTTGG ACAGGAGCCA TATGATCTAC CAAAACATAG CCACATCCCC
    TGTCACTATG ACCTCCTTCC TGCCAGAGAT AGTCCCTCCT CCGAGCTGCA GGAGCCAGGC
    AGTGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011480398.1
    CDS182..3550
    Translation

    Target ORF information:

    RefSeq Version XM_011482096.2
    Organism Oryzias latipes(Japanese medaka)
    Definition Oryzias latipes multiple EGF like domains 10 (megf10), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011482096.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCTCTGT TGCTCCACGG GACACTCCTT TGCACAGCGG TCCTCTGCTT CCTTCCATCC 
    AGCACCTCCT CCCTCAGTGA TGAAGATCCC AACGTCTGCA GCCACTGGGA GAGTTACTCC
    ATGACGGTGC AGGAGACCTA CTCCCATCCT TACGATCAGG TTTTCTACAC CAGCTGCTCA
    GACATCCTGA GCTGGTTCAA ATGCACCACT CACAAAACCA GCTATCGGAT ATCATATCGC
    AGCAGAGAGA AGATCATGTA CCGCAGGAAA TCCCAGTGCT GCCCAGGCTT CTATGAAAAT
    AAAGATGTGT GCACTCCTCG TTGCACAGAG AGCTGTGTTC ACGGCCGCTG CGTGGCCCCC
    AACAGCTGCC AGTGTGAGCC AGGCTGGGGG GGCTCCAACT GCTCCAGCGC CTGTGATAGC
    AGTCACTGGG GTCCACACTG CAGCAACAGA TGTCAGTGCC AGCATGGAGC GCTTTGCAAC
    CCCATTACTG GAGCTTGTGT CTGCAGCCCG GGCTACAGAG GCTGGCGCTG CGAGTCCCAC
    TGTGGAACCG GCACCTACGG CAATGGCTGC CAGCAGAAGT GTCAGTGCCA GAACGGAGCA
    TCCTGTCACC ATGTGACCGG AGAGTGCACG TGCTCAGCCG GATACACCGG AGCTTTCTGT
    GAGGACCTGT GTCCTCCTGG TAAACATGGA CAGCAGTGCG AGGAGAGATG TCCCTGTCAG
    AATGGGGGAG TGTGTCATCA CGTGACTGGA GACTGCTCCT GTCCTGCCGG ATGGAGGGGG
    ACGGTGTGTG GTCAACCGTG TCCAGAGGGA CGGTTTGGTC TGGACTGCTC CCAGGAATGC
    CAGTGCCACA ATGATGCCGT TTGTTTGTCG ACATCCGGGG AGTGTCTCTG CCGTGAGGGA
    TACACCGGAG AGCGGTGTCA GGACCAGTGC CCTGTGGGGA CCTACGGTGC CGGCTGCACA
    AAATCATGTA GCTGTGAGAA CAACAGCAAG TGCGACCACA AGACTGGATC ATGTCTTTGT
    GAGCCGGGCT TCATGGGAGA ATCCTGCCAC GTCCGTTTTT GTCCCGAAGG CCACTTTGGC
    CTGCACTGTG GCAGGAAGTG TCCGTGCCAC CTCCCGAACA CTCGCAGCTG CCACCCGATG
    ACCGGGGAGT GCACGTGCAG ACCCGGTTGG TCGGGTCTGT ACTGCAATGA GACATGTTCT
    CTGGGATTCT ACGGCGAGTC GTGCAAAGAA GTGTGCCAGT GCCAGAACGG AGCCGACTGC
    CACAGCGTGA CCGGGGAGTG TACCTGTGCT CCAGGCTTTA CGGGTCACAG GTGTTCCACT
    CCATGCCCGG CGGGCTCCTA TGGACTGAGC TGCTCATCCA GATGCTTTTG TAAGAACGGA
    GCTCGCTGCT CACCTGTGGA TGGATCCTGC TCCTGTGCAC CAGGCTGGCA TGGGGTGGAC
    TGCTCCATCA GCTGCCCCAG CGGGACATGG GGGCTGGGCT GCAACCTCAC CTGTGCATGT
    GCCAACGGGG GCTCCTGCAG CGCGTTGGAC GGCCGCTGCA CCTGTGCTGC TGGCTGGAGA
    GGAGAGAGGT GTGACCTGCG CTGTCAGGAC GGCACCTATG GTCTGGACTG CAGGGGGCGC
    TGCGACTGCA GCCATTCTGA TGGGTGTCAC CATTCAACCG GCCACTGCCG CTGTCTGCCC
    GGATGGACCG GGATCCACTG TGACAGCGTG TGCGCCGAGG GCCGCTGGGG GCCCAACTGC
    TCCACCCCCT GCAATTGTAA GAACGGAGCA TCCTGCTCTC CAGATGAAGG AACCTGCGAG
    TGTGCTCCAG GATTCAGAGG CACCACCTGT CAGAGGATCT GCTCTCCAGG TTACTTTGGT
    CACCGCTGCA GTCAGACCTG TCCTCAGTGC GTCCACAGTA ACGGGCCGTG CCACCACGTC
    AGTGGACACT GTGACTGTCT GCCTGGTTTC AGGGGGGCGC TGTGCAATGA GGTGTGTCCC
    AGTGGCCACT TTGGGAAAAA CTGCGCCTCG ACCTGCCACT GCACCAACAA CGGCACCTGC
    AACCCCATCG ACGGCTCCTG CCAGTGCTAC CCAGGGTGGA TTGGCAGCGA CTGTTCCCAG
    TCCTGTCCAC CCGGATCCTG GGGACCCAAC TGCATCCACA CCTGCACCTG CCACAACGCC
    GCGGGCTGCA GTGCCTATGA TGGAGAGTGC AAGTGCACCC CTGGGTGGAC GGGTCTCTAC
    TGCACTCAGC GCTGTCCTTT GGGTTTCTAT GGGAAGGAAT GCTCTCAGAG CTGCAGGTGC
    AGAAACGGTG CAGACTGTGA CCACATATCT GGACAGTGCA CGTGTCGCAT CGGCTTCATG
    GGACTACACT GTGAACAAAA GTGTCCTCCT GGCTCCTATG GTTATGGCTG CCGCCATGTC
    TGTGACTGCC TCAATAACTC CACGTGTGAC CACATGAGTG GAACGTGCTA CTGCAGTCCC
    GGCTGGAGAG GCGCTCGATG CGACCAAGCC GCTGCAGTGG TGGTGGGCGA CCTCAACAGT
    TTGACCAGTG TAGCCATGCA CATGGACACC TACCAGATAG GAGTCATCAC AGGAATCATT
    GTCCTGGTTC TGCTGGTGCT CCTCCTCCTG CTCCTCTTCA TCATGTATCG AAAGAAGCAG
    AAGAGCAAAG AGCCCACCAT GCCGCCTGTC AGCTACACAT CAGCAGCCCG GGTCCCACCT
    GATCATCTAG TGGCAGACAC CCCGACCAGC GAGGTCCATC CGAGTTACTT CTCCAACCCC
    AGTTATCACA CGCTGACTCA GTGCATGGGC CCGCCGTACG TCACCGGTGC CTCGTATGGG
    AAGACCAAAT TTGGCCAGCC ATCTGCTGGC ACAAAGAGCC TGGACCAGAG AAAACCAGTC
    CAGTTTGTGG CTCCCACTGG CCCTCTGAGT GCTGAATGGA ATCAAGGGGA CTACATGGAT
    GACCTGGGAG CTTATGGAGT GCAAAGAGGA CACCCTGGAA AGTCTTTAAG AGACCTGGCG
    AAAGGTGCCG CCTATCATGC CAGCACATGC TCCCTAAGCA GCTCGGAAAA TCCATATGCC
    ACAATCAAGG ACCCCCCTCT GCTGACAGCC AGAGGGACAG AATGTGGCTA TGTGGAGATG
    AAATCTCCAG CCAGAAGGGA TTCAGCGTAT GCTGAAATAA GCAACAGCAG CTTGTCCAAC
    AATCGCAATG TCTATGAAGT TGAGCCAACA ACCAGCACCA GCCAGTCCTG TGGAGACAGT
    AGCAGCGTCC TGCAGTTTGG ACAGGAGCCA TATGATCTAC CAAAACATAG CCACATCCCC
    TGTCACTATG ACCTCCTTCC TGCCAGAGAT AGTCCCTCCT CCGAGCTGCA GGAGCCAGGC
    AGTGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.