LOC5576746 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC5576746
Entrez Gene ID	5576746
Full Name	guanine nucleotide exchange factor DBS
Synonyms	AAEL011142,AAEL012730,AAEL012732
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021839976.1	XP_021695668.1	uncharacterized protein LOC5576746 isoform X1
XM_021839977.1	XP_021695669.1	uncharacterized protein LOC5576746 isoform X1
XM_001662793.1	XP_001662843.1	AAEL012730-PA
XM_021839979.1	XP_021695671.1	guanine nucleotide exchange factor DBS isoform X3
XM_021839980.1	XP_021695672.1	uncharacterized protein LOC5576746 isoform X4
XM_021839981.1	XP_021695673.1	uncharacterized protein LOC5576746 isoform X4
XM_021839978.1	XP_021695670.1	uncharacterized protein LOC5576746 isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC5576746 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC5576746 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe118515	XM_021839979.1 Latest version!	Aedes aegypti guanine nucleotide exchange factor DBS (LOC5576746), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAe118516	XM_021839980.1 Latest version!	Aedes aegypti guanine nucleotide exchange factor DBS (LOC5576746), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAe118516	XM_021839981.1 Latest version!	Aedes aegypti guanine nucleotide exchange factor DBS (LOC5576746), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAe11576	XM_001662793.1 Latest version!	Aedes aegypti AAEL012730-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAe118513	XM_021839976.1 Latest version!	Aedes aegypti guanine nucleotide exchange factor DBS (LOC5576746), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAe118513	XM_021839977.1 Latest version!	Aedes aegypti guanine nucleotide exchange factor DBS (LOC5576746), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAe118514	XM_021839978.1 Latest version!	Aedes aegypti guanine nucleotide exchange factor DBS (LOC5576746), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC5576746 ( XM_021839979.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021695671.1 Aedes aegypti guanine nucleotide exchange factor DBS (LOC5576746), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OAe118515
Clone ID Related Accession (Same CDS sequence)	XM_021839979.1
Accession Version	XM_021839979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	guanine nucleotide exchange factor DBS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACAAG CGGAGAGTAA TGAATCTCCC CCGCCATCTG CCAACGGTGG AGGCGGCAGC 
ACGGCATCAT CTTACCACTC GGCGCACGCC CATCATCATC AGCAGCAGCA CCAGCAACAT 
CTGTTCCAGC ATCAATCTTC GGTTACTTCG GTCAGTTCGG TGCAGACAGT GCTCAGTCAG 
AACCTTCAGA ATGCCACCAA CGTGAACAAG CTGATCGAGA ACGTGTGCCA GAATGGGAAC 
CACCTGAACA TGAACACGTT TGGCAGTTGC AGCTCGCTGA TAACCACAGG GCACGTGGTG 
CCTGCGAGTG TGAATGGTGG CCTCACGGGA CAGGGGATGG TTCAGCAGCA ACAACTCGGA 
CCACAGAGGA ATGGTGCTAT ATGCGGAGAG CAGGGCATGA ATTCCAGTGT CAGTTCGGCG 
GAAGAGCCGT TGAGCGTGGC CGATGTGGCT GATTTGCTGC ATCCACAGTA TGCGATCATA 
ACAGGTGGTC GTTCCAAAGA CGGTTGTCCG CTGATAACCT TCCCGGACTA CAATAACTTT 
CACAATCTGT CCGATCTAGA TTACCAAAAA TTAGTGCTAT ATCTCACCTC GGTCCCCTCG 
CTTTCGGAAG CAGATCTCGG CTTCAACCTC ATAATAGACC GACGGAAAGA CCGCTGGGCA 
GCGGTCAAAG CCGTTCTCCT GAAAATCTCC ATCTATTTCC CGGCCATGGT GCACTTCGTG 
TACGTGCTGC GACCGTCCGG GTTCCTACAG AAAGCCATCT CCGAAGTTAG TAACAAATTT 
TTCAAAGACG AATTTCGATT TCGGGTTGTC GTTTGCGCCA CGCTGGAAGA CCTGCACGAG 
TACATCTCGC GAACGCAGCT GACCGGAGAG CTTGGTGGCG AATTGGTTTA CTCGCACCAC 
GACTGGATCC AGCAGCGAAT ATCGCTGGAA AAGTTTTCCG GTTTGACCAA CATGATCTCG 
TGCAACTTGG ACGCGTTCAT GAAGATGATT CACGAGATGG AGTTCCCCAA CTCGGTGGAT 
GCGACCGAGA AGCTGATCGA GGACCAGGGC AATCAGTATG AGAAGTTGAA GGAGGACATT 
ATGGCCGCAG GTCGGCACGG CGAGGTCCTG TTGGAAGAGA TGCGTACCAA AAAAGAAACC 
GACAAGGAAA CTGTCGAACG GTTCGGTAAC ATCAGCTCCA TTGAAAGTGC AGGGAATTAT 
TCAAACAATA CTATGCATCA CCATCTTGAT GACAGTCGCG ATTGTCACAG ACTCTTGGTG 
CAGCTGGAGG AGACCGAGCG GCACTTCGAG GACTTCTGGA CGGTGCACCT GTCGCGGCTG 
AAGAAGTGCC TGGAGCTGAG GCGCTTCGAG CAGGACTTCC GGGAGCTGCA GGCCAACTTC 
GATCGGCACC TGAAGGAGGT GTCGGACATG ACCGAGATCG GCGAAACTGT GGACCGGATG 
GACCAGCTGA TCAAAGAAAC CAAAGACTTC CAAGTGAGCT GCACGGTAGA TGTGGAACGG 
GCCGATCAGG TGATCGCGAC CGGGCAGAGG TTGATTGGTA CCAAAGGTTG TATCAGTTCG 
TGTCCACGGG AAGTGGTGCA ACCGAAATGC GACGAGCTGT CCAGGGTTTG CGAACTGATC 
AACGAGCGCA TTTCCAAGCG AATAGAAACG TTGATCAAGG CCCGGGAGCT GATGGAGCGT 
GTTGAGAAGG CAAACAAGTG GTGCGCTCGG GGCATCGAAC TGTTGGCCAC CCAGCGCATC 
GAAAAGTGTT CCGTGTCGGC GGAAATTGCG GAGCAGAGTC TGCAGGAGAT TCAGGAGTAC 
CTGGCCACGG CGGCCGAGTT CAAGCTATCT AGTCCGAAAG AGTTCAAGAA CGTGATCCAG 
GAGAGTACAA CCCTGGAAAC GAAGGCGCTG GTGTCGCAGG TTCTCCAGCG AATCGACGAC 
GTCCAGCTGA TGTGCGACAA ACGGGTGACC GTGCTCAAGA AACTCACCCT GAAACCTCCC 
CGTCCAGTGC AAACCGTAAC ACCGGAGCCG GCAGTCCCCT TGCAACCCCC CGGAGGAGCA 
CCTCATCCCA TCTTTCGGAC CCGCCGCTCG TACAACAAGA TTGAAAGCTT CTCGTCGAGG 
CCCCGCTCAA TCGAGGGACC CCCGGACTCT GGGATATCCA TCAGCAGCGA TACCATCATT 
GCCAGCTCGG ACAGTGATCT CAATCTGCAG AGTCAAACGC AGGATTCGGA CGAGCGGAAA 
CTGAAACGAG GTCACGTGCT TGCCGAGCTA CTCGAGACGG AACGGATCTA CGTGGCGGAA 
ATGGGATCAA TTTTGAAGGG CTACCGAGAC GAGATGCTGT CGGAGGAGAT GAGTTCACTG 
GTGCCACCAG GACTGCAGGG CAAGGCAGAT ATCTTGTTCG GAAATCTGCA CGAGCTGTAC 
ACCTTCCACA ATGACATTTT CCTGAAGGAT CTGGAGAACT GCATATCCAC CACCGAACTA 
GTCGCATTGT GCTTCGTGCA GAGGCGGGAC ACATTCTTCC GGTTGTATTC GTACTACTGT 
CAGAACATTC CGCGCTCCGA ACGGCTTCGA GAGACCCTTG TGGACACGCA TCTGTTCCTG 
CAGGAGTGCC AGAAGAAGCT CGGCCACAAA CTGCCCCTTG CTGCATATTT GCTCAAACCA 
GTACAAAGGA TCACCAAGTA TCAACTTCTC TTAAAGGATC TGTTAAAATT CAGTGACACC 
GGAACGTGTT CCCGGGAGCT GCAGAAGGCG TTGGACTGTA TGCTGGTGGT GCTCAAGTGC 
GTTAACGATT CGATGCATCA GATTGCAATA ACTGGTTTTC CCGCCGACCT CTCGCAGCAA 
GGGGAACTTC TGCTGCAGGA TTCGTTCCAA GTGTGGACTG AAAGTAAAAA AGATCTCCGG 
TTGCGACTGA AAACCCAACA TCGGCACATT TTCCTCTACC AGAAGGCAAT GCTTTTCTGC 
AAACAGGGTT CGAAAACCGG CCACAACAAG AGTACCTACC AGTTCAAACA CTGGCTACAG 
ATGTCCCAAA TCGGTCTAAC GGAATCGGTC CGAGGCGACC CACGGAGGTT CGAAGTGTGG 
CTCCAAGGCC GACAGGAAGT GCACACGATA CAGGCCAACA CGGTGGAAAT CAAGAACAAG 
TGGGTGGCCG AGATCAAACG GGTGCTGTTG AACCAACTGG AAGAACTCAA GGGGGAGAAA 
ATCAAACAGT ACGGACTGAA TCACAAACCC CTTCGTCACA CGGCCTCGTG GGACCTGCCA 
CCGTCGATAC ATGGGACGCC CAACCGGACG CTGAGCTGTG ACCAGCAGCT ACCCCCACCG 
ACCAATGGCG GCATCGGAAA CATTCAGCAC CACCCAGCGA TGGGCCACCA GCAGGATGTC 
ATCACCCGCA TTTCGCACCT GACCGAGGAC AGCATGATGA GTGCTACGGG CATTTCCTCT 
TCCTCGTCCG AACACGACAA TCAGGAAACG AATGCCTGGA GCTCGGACTA CTCGAATAGT 
GAGGACGAAT TTACGCACGG GGAGGATGGC GTACTGCCGG GTCATAAGTT TGTATCGCTA 
GCGGACTATT GTGCGATGGG AAACTCGGAG GTTTCCATGA AGGAAGGAGA CATTGTGGAG 
CTGCTCAAAA TCGGATGTGC AGGGTGGTGG TTTGTGAAGG TCATCGGCAA TAGTCTCGAA 
GGATGGGCCC CGGCAGCATA CCTAGAGCCT ATCAACCGGA AGTCGTCCAG ACTACGTGGT 
GCTCGCAGCC AAGACAAACT GAACGAACAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021695671.1
CDS	1262..5014
Translation	MGQAESNESPPPSANGGGGSTASSYHSAHAHHHQQQHQQHLFQHQSSVTSVSSVQTVLSQNLQNATNVNKLIENVCQNGNHLNMNTFGSCSSLITTGHVVPASVNGGLTGQGMVQQQQLGPQRNGAICGEQGMNSSVSSAEEPLSVADVADLLHPQYAIITGGRSKDGCPLITFPDYNNFHNLSDLDYQKLVLYLTSVPSLSEADLGFNLIIDRRKDRWAAVKAVLLKISIYFPAMVHFVYVLRPSGFLQKAISEVSNKFFKDEFRFRVVVCATLEDLHEYISRTQLTGELGGELVYSHHDWIQQRISLEKFSGLTNMISCNLDAFMKMIHEMEFPNSVDATEKLIEDQGNQYEKLKEDIMAAGRHGEVLLEEMRTKKETDKETVERFGNISSIESAGNYSNNTMHHHLDDSRDCHRLLVQLEETERHFEDFWTVHLSRLKKCLELRRFEQDFRELQANFDRHLKEVSDMTEIGETVDRMDQLIKETKDFQVSCTVDVERADQVIATGQRLIGTKGCISSCPREVVQPKCDELSRVCELINERISKRIETLIKARELMERVEKANKWCARGIELLATQRIEKCSVSAEIAEQSLQEIQEYLATAAEFKLSSPKEFKNVIQESTTLETKALVSQVLQRIDDVQLMCDKRVTVLKKLTLKPPRPVQTVTPEPAVPLQPPGGAPHPIFRTRRSYNKIESFSSRPRSIEGPPDSGISISSDTIIASSDSDLNLQSQTQDSDERKLKRGHVLAELLETERIYVAEMGSILKGYRDEMLSEEMSSLVPPGLQGKADILFGNLHELYTFHNDIFLKDLENCISTTELVALCFVQRRDTFFRLYSYYCQNIPRSERLRETLVDTHLFLQECQKKLGHKLPLAAYLLKPVQRITKYQLLLKDLLKFSDTGTCSRELQKALDCMLVVLKCVNDSMHQIAITGFPADLSQQGELLLQDSFQVWTESKKDLRLRLKTQHRHIFLYQKAMLFCKQGSKTGHNKSTYQFKHWLQMSQIGLTESVRGDPRRFEVWLQGRQEVHTIQANTVEIKNKWVAEIKRVLLNQLEELKGEKIKQYGLNHKPLRHTASWDLPPSIHGTPNRTLSCDQQLPPPTNGGIGNIQHHPAMGHQQDVITRISHLTEDSMMSATGISSSSSEHDNQETNAWSSDYSNSEDEFTHGEDGVLPGHKFVSLADYCAMGNSEVSMKEGDIVELLKIGCAGWWFVKVIGNSLEGWAPAAYLEPINRKSSRLRGARSQDKLNEH

Target ORF information:

RefSeq Version	XM_021839979.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti guanine nucleotide exchange factor DBS (LOC5576746), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021839979.1

ORF Insert Sequence:

ATGGGACAAG CGGAGAGTAA TGAATCTCCC CCGCCATCTG CCAACGGTGG AGGCGGCAGC 
ACGGCATCAT CTTACCACTC GGCGCACGCC CATCATCATC AGCAGCAGCA CCAGCAACAT 
CTGTTCCAGC ATCAATCTTC GGTTACTTCG GTCAGTTCGG TGCAGACAGT GCTCAGTCAG 
AACCTTCAGA ATGCCACCAA CGTGAACAAG CTGATCGAGA ACGTGTGCCA GAATGGGAAC 
CACCTGAACA TGAACACGTT TGGCAGTTGC AGCTCGCTGA TAACCACAGG GCACGTGGTG 
CCTGCGAGTG TGAATGGTGG CCTCACGGGA CAGGGGATGG TTCAGCAGCA ACAACTCGGA 
CCACAGAGGA ATGGTGCTAT ATGCGGAGAG CAGGGCATGA ATTCCAGTGT CAGTTCGGCG 
GAAGAGCCGT TGAGCGTGGC CGATGTGGCT GATTTGCTGC ATCCACAGTA TGCGATCATA 
ACAGGTGGTC GTTCCAAAGA CGGTTGTCCG CTGATAACCT TCCCGGACTA CAATAACTTT 
CACAATCTGT CCGATCTAGA TTACCAAAAA TTAGTGCTAT ATCTCACCTC GGTCCCCTCG 
CTTTCGGAAG CAGATCTCGG CTTCAACCTC ATAATAGACC GACGGAAAGA CCGCTGGGCA 
GCGGTCAAAG CCGTTCTCCT GAAAATCTCC ATCTATTTCC CGGCCATGGT GCACTTCGTG 
TACGTGCTGC GACCGTCCGG GTTCCTACAG AAAGCCATCT CCGAAGTTAG TAACAAATTT 
TTCAAAGACG AATTTCGATT TCGGGTTGTC GTTTGCGCCA CGCTGGAAGA CCTGCACGAG 
TACATCTCGC GAACGCAGCT GACCGGAGAG CTTGGTGGCG AATTGGTTTA CTCGCACCAC 
GACTGGATCC AGCAGCGAAT ATCGCTGGAA AAGTTTTCCG GTTTGACCAA CATGATCTCG 
TGCAACTTGG ACGCGTTCAT GAAGATGATT CACGAGATGG AGTTCCCCAA CTCGGTGGAT 
GCGACCGAGA AGCTGATCGA GGACCAGGGC AATCAGTATG AGAAGTTGAA GGAGGACATT 
ATGGCCGCAG GTCGGCACGG CGAGGTCCTG TTGGAAGAGA TGCGTACCAA AAAAGAAACC 
GACAAGGAAA CTGTCGAACG GTTCGGTAAC ATCAGCTCCA TTGAAAGTGC AGGGAATTAT 
TCAAACAATA CTATGCATCA CCATCTTGAT GACAGTCGCG ATTGTCACAG ACTCTTGGTG 
CAGCTGGAGG AGACCGAGCG GCACTTCGAG GACTTCTGGA CGGTGCACCT GTCGCGGCTG 
AAGAAGTGCC TGGAGCTGAG GCGCTTCGAG CAGGACTTCC GGGAGCTGCA GGCCAACTTC 
GATCGGCACC TGAAGGAGGT GTCGGACATG ACCGAGATCG GCGAAACTGT GGACCGGATG 
GACCAGCTGA TCAAAGAAAC CAAAGACTTC CAAGTGAGCT GCACGGTAGA TGTGGAACGG 
GCCGATCAGG TGATCGCGAC CGGGCAGAGG TTGATTGGTA CCAAAGGTTG TATCAGTTCG 
TGTCCACGGG AAGTGGTGCA ACCGAAATGC GACGAGCTGT CCAGGGTTTG CGAACTGATC 
AACGAGCGCA TTTCCAAGCG AATAGAAACG TTGATCAAGG CCCGGGAGCT GATGGAGCGT 
GTTGAGAAGG CAAACAAGTG GTGCGCTCGG GGCATCGAAC TGTTGGCCAC CCAGCGCATC 
GAAAAGTGTT CCGTGTCGGC GGAAATTGCG GAGCAGAGTC TGCAGGAGAT TCAGGAGTAC 
CTGGCCACGG CGGCCGAGTT CAAGCTATCT AGTCCGAAAG AGTTCAAGAA CGTGATCCAG 
GAGAGTACAA CCCTGGAAAC GAAGGCGCTG GTGTCGCAGG TTCTCCAGCG AATCGACGAC 
GTCCAGCTGA TGTGCGACAA ACGGGTGACC GTGCTCAAGA AACTCACCCT GAAACCTCCC 
CGTCCAGTGC AAACCGTAAC ACCGGAGCCG GCAGTCCCCT TGCAACCCCC CGGAGGAGCA 
CCTCATCCCA TCTTTCGGAC CCGCCGCTCG TACAACAAGA TTGAAAGCTT CTCGTCGAGG 
CCCCGCTCAA TCGAGGGACC CCCGGACTCT GGGATATCCA TCAGCAGCGA TACCATCATT 
GCCAGCTCGG ACAGTGATCT CAATCTGCAG AGTCAAACGC AGGATTCGGA CGAGCGGAAA 
CTGAAACGAG GTCACGTGCT TGCCGAGCTA CTCGAGACGG AACGGATCTA CGTGGCGGAA 
ATGGGATCAA TTTTGAAGGG CTACCGAGAC GAGATGCTGT CGGAGGAGAT GAGTTCACTG 
GTGCCACCAG GACTGCAGGG CAAGGCAGAT ATCTTGTTCG GAAATCTGCA CGAGCTGTAC 
ACCTTCCACA ATGACATTTT CCTGAAGGAT CTGGAGAACT GCATATCCAC CACCGAACTA 
GTCGCATTGT GCTTCGTGCA GAGGCGGGAC ACATTCTTCC GGTTGTATTC GTACTACTGT 
CAGAACATTC CGCGCTCCGA ACGGCTTCGA GAGACCCTTG TGGACACGCA TCTGTTCCTG 
CAGGAGTGCC AGAAGAAGCT CGGCCACAAA CTGCCCCTTG CTGCATATTT GCTCAAACCA 
GTACAAAGGA TCACCAAGTA TCAACTTCTC TTAAAGGATC TGTTAAAATT CAGTGACACC 
GGAACGTGTT CCCGGGAGCT GCAGAAGGCG TTGGACTGTA TGCTGGTGGT GCTCAAGTGC 
GTTAACGATT CGATGCATCA GATTGCAATA ACTGGTTTTC CCGCCGACCT CTCGCAGCAA 
GGGGAACTTC TGCTGCAGGA TTCGTTCCAA GTGTGGACTG AAAGTAAAAA AGATCTCCGG 
TTGCGACTGA AAACCCAACA TCGGCACATT TTCCTCTACC AGAAGGCAAT GCTTTTCTGC 
AAACAGGGTT CGAAAACCGG CCACAACAAG AGTACCTACC AGTTCAAACA CTGGCTACAG 
ATGTCCCAAA TCGGTCTAAC GGAATCGGTC CGAGGCGACC CACGGAGGTT CGAAGTGTGG 
CTCCAAGGCC GACAGGAAGT GCACACGATA CAGGCCAACA CGGTGGAAAT CAAGAACAAG 
TGGGTGGCCG AGATCAAACG GGTGCTGTTG AACCAACTGG AAGAACTCAA GGGGGAGAAA 
ATCAAACAGT ACGGACTGAA TCACAAACCC CTTCGTCACA CGGCCTCGTG GGACCTGCCA 
CCGTCGATAC ATGGGACGCC CAACCGGACG CTGAGCTGTG ACCAGCAGCT ACCCCCACCG 
ACCAATGGCG GCATCGGAAA CATTCAGCAC CACCCAGCGA TGGGCCACCA GCAGGATGTC 
ATCACCCGCA TTTCGCACCT GACCGAGGAC AGCATGATGA GTGCTACGGG CATTTCCTCT 
TCCTCGTCCG AACACGACAA TCAGGAAACG AATGCCTGGA GCTCGGACTA CTCGAATAGT 
GAGGACGAAT TTACGCACGG GGAGGATGGC GTACTGCCGG GTCATAAGTT TGTATCGCTA 
GCGGACTATT GTGCGATGGG AAACTCGGAG GTTTCCATGA AGGAAGGAGA CATTGTGGAG 
CTGCTCAAAA TCGGATGTGC AGGGTGGTGG TTTGTGAAGG TCATCGGCAA TAGTCTCGAA 
GGATGGGCCC CGGCAGCATA CCTAGAGCCT ATCAACCGGA AGTCGTCCAG ACTACGTGGT 
GCTCGCAGCC AAGACAAACT GAACGAACAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5576746 ( XM_021839980.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021695672.1 Aedes aegypti guanine nucleotide exchange factor DBS (LOC5576746), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OAe118516
Clone ID Related Accession (Same CDS sequence)	XM_021839980.1 , XM_021839981.1
Accession Version	XM_021839980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	uncharacterized protein LOC5576746 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTCG GTAACTACAA CCTGACGACG GAAACGGCAA ACAGAAGGCA TCTCTTTCTT 
TTTCGCATCA AAAAAGTGAA GAGCACTGCA AGCTACATCG TTGGCAAAGT GCACCGGAAA 
ATCGGCACAG TGAAACGCAA AATGCACCAA AGGCACCAGC ATCGACACCA ACCGTCTCCA 
GTTCCGGCCA TTGCCGCCAT CCCAGATGGT TCCACCTCAG TCACCAGCAC CACTGCCACC 
AGCATCAGCC GTCGAAGGCC TCCGGGGTTG AACCGTTCCG AGTCTCCGTT GTCCTACCGA 
GCGTCCCTGT TTGACGAAAG GAACATCTGC GGCAGCGAAT TGAGTCGTTC GAACGTCAGT 
CGAACTAAGT TTTCACCTCA GGACTTTCCC ACCACATGGA TGGAAGGAGG CAACTCATTC 
GATCCATGTT TTGGTCAGGG AAAGATTGCC GGACAAGATG AAACGTTTCA GGTTTTTAGC 
AACAAAGCAC ATTTATTTCA CAAAAATAAA ACTATATCAG GAAGAAGGCC TCTGTTCGTG 
AAAAACTTTC AGCCTTTCAA TTCACTGCAA CGGGAAATCT CTTTGGCTAA AAAACAGCAC 
AATTTTGGCA GTTGTCCGGA AATCAACTCT GGCACATTGA AATCACAGAT TTCCCAGAAA 
TCTAAGCACT CACAAAAATC ACTGAAAAGT TTCAAATCAA ACAAGTCGTC CAGATATTGC 
AAAAGCAAGA TTGTCCAACT TTTGGAACGG AACAATGACG AAAAAACGGA AATGGATATT 
CTTCAGGAAT ACTTTGATAC GATGTCTTAT ACGGAAATCG TCAAAGATAA AGATTTCAAG 
AACTATCTTA TCAAGAAACA ATACATGGAC ATAATGGATT ACATATATAA CAGTTCCGGG 
TCGGAGTTGG GGAACTCAGA AATACGATCC ATTCCAGCGT CAATACAAGT ATCCAATAAC 
GATTGCGATT CGGTCAAAAA GGAAAAACTC ATGGCAGAAC TACCGAAGAA ATACTATTCG 
TACACCAATC CGCAAGTTTT CTGCAAGCAA GACCAGAACT TTGAGCGATA CTATCAAAAA 
TATGGCACAG TCCCACCGAA AGAATCGCAA GTATTCGGTT TCTCTTCGCT TCCCTCGCAG 
TACTACACCT TGGATCGTTT AGTTCAGACG CCGAAAGAGG ATATTCCTTG TTACGTAACA 
CTACCGAAGA AACCATCACG ATGTAAACGA ACTTATCAAC AGGTCCGTGA ATTTTGCGAA 
AAATCTCTTC TGAAGTATAC AAGCACGAAA CCAACCAGTG ATACATTGAA AATTCAGAAA 
GCTTCCAAGG AGTATACTGA AAAAGCCTAT AACCGAATTA TCAATGATTT CGTTCGAAAA 
CGAGGCTTCA CCAGCGTAGA CGAGTACCTT CAGTATTACT ACGGTAACTT CCTGAATCAG 
AAACCATTCC GCGTGAAGAA ACCGAACCAA TTCAATTCGC TTCCACCCCC GCCATACTCC 
AGTGGAGACT TCCATGAAAA TCACCTCCGA TCGCTAGAAT GCAAACCAGT TTCTCCTTCC 
CGAACCAGTG CAACCATAGC TAACGATTCG GGCGTAGATT GTAGTTCCAA CTTCACCAGT 
CTCATTTACT CCGATTCGTT CAAGAACTTT GACTACGTCA ACCTCACCAC CATGAATCGC 
AAAACCCGCA AGCGTTCTTC TTCGGCATGC TGTCACATGA ATGAGAAACC GCGCTTCTTC 
CTTCCGGTGG GAAGTGACGA CGAGGACATC GACGAAGAAC GGTTCACCTC GCATGTGCTT 
CTCGACGGCC TGATTGCCAC CAGCGACCAA ACTAATCTGC CCTATATCTT CGACGATATC 
GGGATCTATG AGAGTATCGA GTCGAACGAG AAGGAACGCT TCCGTGCCGA AGTGCGCTCC 
CGTAAGGATA GCCTGGTGGA GCGGAGTGGG GCACTGCTTC CGGCGTCAAC GAAAGCAGAG 
CCCGCCATCA CAGGAGGAGT TCGGCGAGGT GCGGATGCAG TGCCACCTCC AAAACCGAGC 
AGGCTGTTGA ACAAGGCAGT GGCGCCCGGG AAGAAACTGC TCAATGAACG GGCTACCGCG 
GGATGTGTTG CGCCGGAAAC TGCTCGGGAT GATGGCTACC GAGACGAGAT GCTGTCGGAG 
GAGATGAGTT CACTGGTGCC ACCAGGACTG CAGGGCAAGG CAGATATCTT GTTCGGAAAT 
CTGCACGAGC TGTACACCTT CCACAATGAC ATTTTCCTGA AGGATCTGGA GAACTGCATA 
TCCACCACCG AACTAGTCGC ATTGTGCTTC GTGCAGAGGC GGGACACATT CTTCCGGTTG 
TATTCGTACT ACTGTCAGAA CATTCCGCGC TCCGAACGGC TTCGAGAGAC CCTTGTGGAC 
ACGCATCTGT TCCTGCAGGA GTGCCAGAAG AAGCTCGGCC ACAAACTGCC CCTTGCTGCA 
TATTTGCTCA AACCAGTACA AAGGATCACC AAGTATCAAC TTCTCTTAAA GGATCTGTTA 
AAATTCAGTG ACACCGGAAC GTGTTCCCGG GAGCTGCAGA AGGCGTTGGA CTGTATGCTG 
GTGGTGCTCA AGTGCGTTAA CGATTCGATG CATCAGATTG CAATAACTGG TTTTCCCGCC 
GACCTCTCGC AGCAAGGGGA ACTTCTGCTG CAGGATTCGT TCCAAGTGTG GACTGAAAGT 
AAAAAAGATC TCCGGTTGCG ACTGAAAACC CAACATCGGC ACATTTTCCT CTACCAGAAG 
GCAATGCTTT TCTGCAAACA GGGTTCGAAA ACCGGCCACA ACAAGAGTAC CTACCAGTTC 
AAACACTGGC TACAGATGTC CCAAATCGGT CTAACGGAAT CGGTCCGAGG CGACCCACGG 
AGGTTCGAAG TGTGGCTCCA AGGCCGACAG GAAGTGCACA CGATACAGGC CAACACGGTG 
GAAATCAAGA ACAAGTGGGT GGCCGAGATC AAACGGGTGC TGTTGAACCA ACTGGAAGAA 
CTCAAGGGGG AGAAAATCAA ACAGTACGGA CTGAATCACA AACCCCTTCG TCACACGGCC 
TCGTGGGACC TGCCACCGTC GATACATGGG ACGCCCAACC GGACGCTGAG CTGTGACCAG 
CAGCTACCCC CACCGACCAA TGGCGGCATC GGAAACATTC AGCACCACCC AGCGATGGGC 
CACCAGCAGG ATGTCATCAC CCGCATTTCG CACCTGACCG AGGACAGCAT GATGAGTGCT 
ACGGGCATTT CCTCTTCCTC GTCCGAACAC GACAATCAGG AAACGAATGC CTGGAGCTCG 
GACTACTCGA ATAGTGAGGA CGAATTTACG CACGGGGAGG ATGGCGTACT GCCGGGTCAT 
AAGTTTGTAT CGCTAGCGGA CTATTGTGCG ATGGGAAACT CGGAGGTTTC CATGAAGGAA 
GGAGACATTG TGGAGCTGCT CAAAATCGGA TGTGCAGGGT GGTGGTTTGT GAAGGTCATC 
GGCAATAGTC TCGAAGGATG GGCCCCGGCA GCATACCTAG AGCCTATCAA CCGGAAGTCG 
TCCAGACTAC GTGGTGCTCG CAGCCAAGAC AAACTGAACG AACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021695672.1
CDS	940..4527
Translation	MKFGNYNLTTETANRRHLFLFRIKKVKSTASYIVGKVHRKIGTVKRKMHQRHQHRHQPSPVPAIAAIPDGSTSVTSTTATSISRRRPPGLNRSESPLSYRASLFDERNICGSELSRSNVSRTKFSPQDFPTTWMEGGNSFDPCFGQGKIAGQDETFQVFSNKAHLFHKNKTISGRRPLFVKNFQPFNSLQREISLAKKQHNFGSCPEINSGTLKSQISQKSKHSQKSLKSFKSNKSSRYCKSKIVQLLERNNDEKTEMDILQEYFDTMSYTEIVKDKDFKNYLIKKQYMDIMDYIYNSSGSELGNSEIRSIPASIQVSNNDCDSVKKEKLMAELPKKYYSYTNPQVFCKQDQNFERYYQKYGTVPPKESQVFGFSSLPSQYYTLDRLVQTPKEDIPCYVTLPKKPSRCKRTYQQVREFCEKSLLKYTSTKPTSDTLKIQKASKEYTEKAYNRIINDFVRKRGFTSVDEYLQYYYGNFLNQKPFRVKKPNQFNSLPPPPYSSGDFHENHLRSLECKPVSPSRTSATIANDSGVDCSSNFTSLIYSDSFKNFDYVNLTTMNRKTRKRSSSACCHMNEKPRFFLPVGSDDEDIDEERFTSHVLLDGLIATSDQTNLPYIFDDIGIYESIESNEKERFRAEVRSRKDSLVERSGALLPASTKAEPAITGGVRRGADAVPPPKPSRLLNKAVAPGKKLLNERATAGCVAPETARDDGYRDEMLSEEMSSLVPPGLQGKADILFGNLHELYTFHNDIFLKDLENCISTTELVALCFVQRRDTFFRLYSYYCQNIPRSERLRETLVDTHLFLQECQKKLGHKLPLAAYLLKPVQRITKYQLLLKDLLKFSDTGTCSRELQKALDCMLVVLKCVNDSMHQIAITGFPADLSQQGELLLQDSFQVWTESKKDLRLRLKTQHRHIFLYQKAMLFCKQGSKTGHNKSTYQFKHWLQMSQIGLTESVRGDPRRFEVWLQGRQEVHTIQANTVEIKNKWVAEIKRVLLNQLEELKGEKIKQYGLNHKPLRHTASWDLPPSIHGTPNRTLSCDQQLPPPTNGGIGNIQHHPAMGHQQDVITRISHLTEDSMMSATGISSSSSEHDNQETNAWSSDYSNSEDEFTHGEDGVLPGHKFVSLADYCAMGNSEVSMKEGDIVELLKIGCAGWWFVKVIGNSLEGWAPAAYLEPINRKSSRLRGARSQDKLNEH

Target ORF information:

RefSeq Version	XM_021839980.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti guanine nucleotide exchange factor DBS (LOC5576746), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021839980.1

ORF Insert Sequence:

ATGAAGTTCG GTAACTACAA CCTGACGACG GAAACGGCAA ACAGAAGGCA TCTCTTTCTT 
TTTCGCATCA AAAAAGTGAA GAGCACTGCA AGCTACATCG TTGGCAAAGT GCACCGGAAA 
ATCGGCACAG TGAAACGCAA AATGCACCAA AGGCACCAGC ATCGACACCA ACCGTCTCCA 
GTTCCGGCCA TTGCCGCCAT CCCAGATGGT TCCACCTCAG TCACCAGCAC CACTGCCACC 
AGCATCAGCC GTCGAAGGCC TCCGGGGTTG AACCGTTCCG AGTCTCCGTT GTCCTACCGA 
GCGTCCCTGT TTGACGAAAG GAACATCTGC GGCAGCGAAT TGAGTCGTTC GAACGTCAGT 
CGAACTAAGT TTTCACCTCA GGACTTTCCC ACCACATGGA TGGAAGGAGG CAACTCATTC 
GATCCATGTT TTGGTCAGGG AAAGATTGCC GGACAAGATG AAACGTTTCA GGTTTTTAGC 
AACAAAGCAC ATTTATTTCA CAAAAATAAA ACTATATCAG GAAGAAGGCC TCTGTTCGTG 
AAAAACTTTC AGCCTTTCAA TTCACTGCAA CGGGAAATCT CTTTGGCTAA AAAACAGCAC 
AATTTTGGCA GTTGTCCGGA AATCAACTCT GGCACATTGA AATCACAGAT TTCCCAGAAA 
TCTAAGCACT CACAAAAATC ACTGAAAAGT TTCAAATCAA ACAAGTCGTC CAGATATTGC 
AAAAGCAAGA TTGTCCAACT TTTGGAACGG AACAATGACG AAAAAACGGA AATGGATATT 
CTTCAGGAAT ACTTTGATAC GATGTCTTAT ACGGAAATCG TCAAAGATAA AGATTTCAAG 
AACTATCTTA TCAAGAAACA ATACATGGAC ATAATGGATT ACATATATAA CAGTTCCGGG 
TCGGAGTTGG GGAACTCAGA AATACGATCC ATTCCAGCGT CAATACAAGT ATCCAATAAC 
GATTGCGATT CGGTCAAAAA GGAAAAACTC ATGGCAGAAC TACCGAAGAA ATACTATTCG 
TACACCAATC CGCAAGTTTT CTGCAAGCAA GACCAGAACT TTGAGCGATA CTATCAAAAA 
TATGGCACAG TCCCACCGAA AGAATCGCAA GTATTCGGTT TCTCTTCGCT TCCCTCGCAG 
TACTACACCT TGGATCGTTT AGTTCAGACG CCGAAAGAGG ATATTCCTTG TTACGTAACA 
CTACCGAAGA AACCATCACG ATGTAAACGA ACTTATCAAC AGGTCCGTGA ATTTTGCGAA 
AAATCTCTTC TGAAGTATAC AAGCACGAAA CCAACCAGTG ATACATTGAA AATTCAGAAA 
GCTTCCAAGG AGTATACTGA AAAAGCCTAT AACCGAATTA TCAATGATTT CGTTCGAAAA 
CGAGGCTTCA CCAGCGTAGA CGAGTACCTT CAGTATTACT ACGGTAACTT CCTGAATCAG 
AAACCATTCC GCGTGAAGAA ACCGAACCAA TTCAATTCGC TTCCACCCCC GCCATACTCC 
AGTGGAGACT TCCATGAAAA TCACCTCCGA TCGCTAGAAT GCAAACCAGT TTCTCCTTCC 
CGAACCAGTG CAACCATAGC TAACGATTCG GGCGTAGATT GTAGTTCCAA CTTCACCAGT 
CTCATTTACT CCGATTCGTT CAAGAACTTT GACTACGTCA ACCTCACCAC CATGAATCGC 
AAAACCCGCA AGCGTTCTTC TTCGGCATGC TGTCACATGA ATGAGAAACC GCGCTTCTTC 
CTTCCGGTGG GAAGTGACGA CGAGGACATC GACGAAGAAC GGTTCACCTC GCATGTGCTT 
CTCGACGGCC TGATTGCCAC CAGCGACCAA ACTAATCTGC CCTATATCTT CGACGATATC 
GGGATCTATG AGAGTATCGA GTCGAACGAG AAGGAACGCT TCCGTGCCGA AGTGCGCTCC 
CGTAAGGATA GCCTGGTGGA GCGGAGTGGG GCACTGCTTC CGGCGTCAAC GAAAGCAGAG 
CCCGCCATCA CAGGAGGAGT TCGGCGAGGT GCGGATGCAG TGCCACCTCC AAAACCGAGC 
AGGCTGTTGA ACAAGGCAGT GGCGCCCGGG AAGAAACTGC TCAATGAACG GGCTACCGCG 
GGATGTGTTG CGCCGGAAAC TGCTCGGGAT GATGGCTACC GAGACGAGAT GCTGTCGGAG 
GAGATGAGTT CACTGGTGCC ACCAGGACTG CAGGGCAAGG CAGATATCTT GTTCGGAAAT 
CTGCACGAGC TGTACACCTT CCACAATGAC ATTTTCCTGA AGGATCTGGA GAACTGCATA 
TCCACCACCG AACTAGTCGC ATTGTGCTTC GTGCAGAGGC GGGACACATT CTTCCGGTTG 
TATTCGTACT ACTGTCAGAA CATTCCGCGC TCCGAACGGC TTCGAGAGAC CCTTGTGGAC 
ACGCATCTGT TCCTGCAGGA GTGCCAGAAG AAGCTCGGCC ACAAACTGCC CCTTGCTGCA 
TATTTGCTCA AACCAGTACA AAGGATCACC AAGTATCAAC TTCTCTTAAA GGATCTGTTA 
AAATTCAGTG ACACCGGAAC GTGTTCCCGG GAGCTGCAGA AGGCGTTGGA CTGTATGCTG 
GTGGTGCTCA AGTGCGTTAA CGATTCGATG CATCAGATTG CAATAACTGG TTTTCCCGCC 
GACCTCTCGC AGCAAGGGGA ACTTCTGCTG CAGGATTCGT TCCAAGTGTG GACTGAAAGT 
AAAAAAGATC TCCGGTTGCG ACTGAAAACC CAACATCGGC ACATTTTCCT CTACCAGAAG 
GCAATGCTTT TCTGCAAACA GGGTTCGAAA ACCGGCCACA ACAAGAGTAC CTACCAGTTC 
AAACACTGGC TACAGATGTC CCAAATCGGT CTAACGGAAT CGGTCCGAGG CGACCCACGG 
AGGTTCGAAG TGTGGCTCCA AGGCCGACAG GAAGTGCACA CGATACAGGC CAACACGGTG 
GAAATCAAGA ACAAGTGGGT GGCCGAGATC AAACGGGTGC TGTTGAACCA ACTGGAAGAA 
CTCAAGGGGG AGAAAATCAA ACAGTACGGA CTGAATCACA AACCCCTTCG TCACACGGCC 
TCGTGGGACC TGCCACCGTC GATACATGGG ACGCCCAACC GGACGCTGAG CTGTGACCAG 
CAGCTACCCC CACCGACCAA TGGCGGCATC GGAAACATTC AGCACCACCC AGCGATGGGC 
CACCAGCAGG ATGTCATCAC CCGCATTTCG CACCTGACCG AGGACAGCAT GATGAGTGCT 
ACGGGCATTT CCTCTTCCTC GTCCGAACAC GACAATCAGG AAACGAATGC CTGGAGCTCG 
GACTACTCGA ATAGTGAGGA CGAATTTACG CACGGGGAGG ATGGCGTACT GCCGGGTCAT 
AAGTTTGTAT CGCTAGCGGA CTATTGTGCG ATGGGAAACT CGGAGGTTTC CATGAAGGAA 
GGAGACATTG TGGAGCTGCT CAAAATCGGA TGTGCAGGGT GGTGGTTTGT GAAGGTCATC 
GGCAATAGTC TCGAAGGATG GGCCCCGGCA GCATACCTAG AGCCTATCAA CCGGAAGTCG 
TCCAGACTAC GTGGTGCTCG CAGCCAAGAC AAACTGAACG AACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5576746 ( XM_021839981.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021695673.1 Aedes aegypti guanine nucleotide exchange factor DBS (LOC5576746), transcript variant X6, mRNA.

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CloneID	OAe118516
Clone ID Related Accession (Same CDS sequence)	XM_021839980.1 , XM_021839981.1
Accession Version	XM_021839981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	uncharacterized protein LOC5576746 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTCG GTAACTACAA CCTGACGACG GAAACGGCAA ACAGAAGGCA TCTCTTTCTT 
TTTCGCATCA AAAAAGTGAA GAGCACTGCA AGCTACATCG TTGGCAAAGT GCACCGGAAA 
ATCGGCACAG TGAAACGCAA AATGCACCAA AGGCACCAGC ATCGACACCA ACCGTCTCCA 
GTTCCGGCCA TTGCCGCCAT CCCAGATGGT TCCACCTCAG TCACCAGCAC CACTGCCACC 
AGCATCAGCC GTCGAAGGCC TCCGGGGTTG AACCGTTCCG AGTCTCCGTT GTCCTACCGA 
GCGTCCCTGT TTGACGAAAG GAACATCTGC GGCAGCGAAT TGAGTCGTTC GAACGTCAGT 
CGAACTAAGT TTTCACCTCA GGACTTTCCC ACCACATGGA TGGAAGGAGG CAACTCATTC 
GATCCATGTT TTGGTCAGGG AAAGATTGCC GGACAAGATG AAACGTTTCA GGTTTTTAGC 
AACAAAGCAC ATTTATTTCA CAAAAATAAA ACTATATCAG GAAGAAGGCC TCTGTTCGTG 
AAAAACTTTC AGCCTTTCAA TTCACTGCAA CGGGAAATCT CTTTGGCTAA AAAACAGCAC 
AATTTTGGCA GTTGTCCGGA AATCAACTCT GGCACATTGA AATCACAGAT TTCCCAGAAA 
TCTAAGCACT CACAAAAATC ACTGAAAAGT TTCAAATCAA ACAAGTCGTC CAGATATTGC 
AAAAGCAAGA TTGTCCAACT TTTGGAACGG AACAATGACG AAAAAACGGA AATGGATATT 
CTTCAGGAAT ACTTTGATAC GATGTCTTAT ACGGAAATCG TCAAAGATAA AGATTTCAAG 
AACTATCTTA TCAAGAAACA ATACATGGAC ATAATGGATT ACATATATAA CAGTTCCGGG 
TCGGAGTTGG GGAACTCAGA AATACGATCC ATTCCAGCGT CAATACAAGT ATCCAATAAC 
GATTGCGATT CGGTCAAAAA GGAAAAACTC ATGGCAGAAC TACCGAAGAA ATACTATTCG 
TACACCAATC CGCAAGTTTT CTGCAAGCAA GACCAGAACT TTGAGCGATA CTATCAAAAA 
TATGGCACAG TCCCACCGAA AGAATCGCAA GTATTCGGTT TCTCTTCGCT TCCCTCGCAG 
TACTACACCT TGGATCGTTT AGTTCAGACG CCGAAAGAGG ATATTCCTTG TTACGTAACA 
CTACCGAAGA AACCATCACG ATGTAAACGA ACTTATCAAC AGGTCCGTGA ATTTTGCGAA 
AAATCTCTTC TGAAGTATAC AAGCACGAAA CCAACCAGTG ATACATTGAA AATTCAGAAA 
GCTTCCAAGG AGTATACTGA AAAAGCCTAT AACCGAATTA TCAATGATTT CGTTCGAAAA 
CGAGGCTTCA CCAGCGTAGA CGAGTACCTT CAGTATTACT ACGGTAACTT CCTGAATCAG 
AAACCATTCC GCGTGAAGAA ACCGAACCAA TTCAATTCGC TTCCACCCCC GCCATACTCC 
AGTGGAGACT TCCATGAAAA TCACCTCCGA TCGCTAGAAT GCAAACCAGT TTCTCCTTCC 
CGAACCAGTG CAACCATAGC TAACGATTCG GGCGTAGATT GTAGTTCCAA CTTCACCAGT 
CTCATTTACT CCGATTCGTT CAAGAACTTT GACTACGTCA ACCTCACCAC CATGAATCGC 
AAAACCCGCA AGCGTTCTTC TTCGGCATGC TGTCACATGA ATGAGAAACC GCGCTTCTTC 
CTTCCGGTGG GAAGTGACGA CGAGGACATC GACGAAGAAC GGTTCACCTC GCATGTGCTT 
CTCGACGGCC TGATTGCCAC CAGCGACCAA ACTAATCTGC CCTATATCTT CGACGATATC 
GGGATCTATG AGAGTATCGA GTCGAACGAG AAGGAACGCT TCCGTGCCGA AGTGCGCTCC 
CGTAAGGATA GCCTGGTGGA GCGGAGTGGG GCACTGCTTC CGGCGTCAAC GAAAGCAGAG 
CCCGCCATCA CAGGAGGAGT TCGGCGAGGT GCGGATGCAG TGCCACCTCC AAAACCGAGC 
AGGCTGTTGA ACAAGGCAGT GGCGCCCGGG AAGAAACTGC TCAATGAACG GGCTACCGCG 
GGATGTGTTG CGCCGGAAAC TGCTCGGGAT GATGGCTACC GAGACGAGAT GCTGTCGGAG 
GAGATGAGTT CACTGGTGCC ACCAGGACTG CAGGGCAAGG CAGATATCTT GTTCGGAAAT 
CTGCACGAGC TGTACACCTT CCACAATGAC ATTTTCCTGA AGGATCTGGA GAACTGCATA 
TCCACCACCG AACTAGTCGC ATTGTGCTTC GTGCAGAGGC GGGACACATT CTTCCGGTTG 
TATTCGTACT ACTGTCAGAA CATTCCGCGC TCCGAACGGC TTCGAGAGAC CCTTGTGGAC 
ACGCATCTGT TCCTGCAGGA GTGCCAGAAG AAGCTCGGCC ACAAACTGCC CCTTGCTGCA 
TATTTGCTCA AACCAGTACA AAGGATCACC AAGTATCAAC TTCTCTTAAA GGATCTGTTA 
AAATTCAGTG ACACCGGAAC GTGTTCCCGG GAGCTGCAGA AGGCGTTGGA CTGTATGCTG 
GTGGTGCTCA AGTGCGTTAA CGATTCGATG CATCAGATTG CAATAACTGG TTTTCCCGCC 
GACCTCTCGC AGCAAGGGGA ACTTCTGCTG CAGGATTCGT TCCAAGTGTG GACTGAAAGT 
AAAAAAGATC TCCGGTTGCG ACTGAAAACC CAACATCGGC ACATTTTCCT CTACCAGAAG 
GCAATGCTTT TCTGCAAACA GGGTTCGAAA ACCGGCCACA ACAAGAGTAC CTACCAGTTC 
AAACACTGGC TACAGATGTC CCAAATCGGT CTAACGGAAT CGGTCCGAGG CGACCCACGG 
AGGTTCGAAG TGTGGCTCCA AGGCCGACAG GAAGTGCACA CGATACAGGC CAACACGGTG 
GAAATCAAGA ACAAGTGGGT GGCCGAGATC AAACGGGTGC TGTTGAACCA ACTGGAAGAA 
CTCAAGGGGG AGAAAATCAA ACAGTACGGA CTGAATCACA AACCCCTTCG TCACACGGCC 
TCGTGGGACC TGCCACCGTC GATACATGGG ACGCCCAACC GGACGCTGAG CTGTGACCAG 
CAGCTACCCC CACCGACCAA TGGCGGCATC GGAAACATTC AGCACCACCC AGCGATGGGC 
CACCAGCAGG ATGTCATCAC CCGCATTTCG CACCTGACCG AGGACAGCAT GATGAGTGCT 
ACGGGCATTT CCTCTTCCTC GTCCGAACAC GACAATCAGG AAACGAATGC CTGGAGCTCG 
GACTACTCGA ATAGTGAGGA CGAATTTACG CACGGGGAGG ATGGCGTACT GCCGGGTCAT 
AAGTTTGTAT CGCTAGCGGA CTATTGTGCG ATGGGAAACT CGGAGGTTTC CATGAAGGAA 
GGAGACATTG TGGAGCTGCT CAAAATCGGA TGTGCAGGGT GGTGGTTTGT GAAGGTCATC 
GGCAATAGTC TCGAAGGATG GGCCCCGGCA GCATACCTAG AGCCTATCAA CCGGAAGTCG 
TCCAGACTAC GTGGTGCTCG CAGCCAAGAC AAACTGAACG AACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021695673.1
CDS	558..4145
Translation	MKFGNYNLTTETANRRHLFLFRIKKVKSTASYIVGKVHRKIGTVKRKMHQRHQHRHQPSPVPAIAAIPDGSTSVTSTTATSISRRRPPGLNRSESPLSYRASLFDERNICGSELSRSNVSRTKFSPQDFPTTWMEGGNSFDPCFGQGKIAGQDETFQVFSNKAHLFHKNKTISGRRPLFVKNFQPFNSLQREISLAKKQHNFGSCPEINSGTLKSQISQKSKHSQKSLKSFKSNKSSRYCKSKIVQLLERNNDEKTEMDILQEYFDTMSYTEIVKDKDFKNYLIKKQYMDIMDYIYNSSGSELGNSEIRSIPASIQVSNNDCDSVKKEKLMAELPKKYYSYTNPQVFCKQDQNFERYYQKYGTVPPKESQVFGFSSLPSQYYTLDRLVQTPKEDIPCYVTLPKKPSRCKRTYQQVREFCEKSLLKYTSTKPTSDTLKIQKASKEYTEKAYNRIINDFVRKRGFTSVDEYLQYYYGNFLNQKPFRVKKPNQFNSLPPPPYSSGDFHENHLRSLECKPVSPSRTSATIANDSGVDCSSNFTSLIYSDSFKNFDYVNLTTMNRKTRKRSSSACCHMNEKPRFFLPVGSDDEDIDEERFTSHVLLDGLIATSDQTNLPYIFDDIGIYESIESNEKERFRAEVRSRKDSLVERSGALLPASTKAEPAITGGVRRGADAVPPPKPSRLLNKAVAPGKKLLNERATAGCVAPETARDDGYRDEMLSEEMSSLVPPGLQGKADILFGNLHELYTFHNDIFLKDLENCISTTELVALCFVQRRDTFFRLYSYYCQNIPRSERLRETLVDTHLFLQECQKKLGHKLPLAAYLLKPVQRITKYQLLLKDLLKFSDTGTCSRELQKALDCMLVVLKCVNDSMHQIAITGFPADLSQQGELLLQDSFQVWTESKKDLRLRLKTQHRHIFLYQKAMLFCKQGSKTGHNKSTYQFKHWLQMSQIGLTESVRGDPRRFEVWLQGRQEVHTIQANTVEIKNKWVAEIKRVLLNQLEELKGEKIKQYGLNHKPLRHTASWDLPPSIHGTPNRTLSCDQQLPPPTNGGIGNIQHHPAMGHQQDVITRISHLTEDSMMSATGISSSSSEHDNQETNAWSSDYSNSEDEFTHGEDGVLPGHKFVSLADYCAMGNSEVSMKEGDIVELLKIGCAGWWFVKVIGNSLEGWAPAAYLEPINRKSSRLRGARSQDKLNEH

Target ORF information:

RefSeq Version	XM_021839981.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti guanine nucleotide exchange factor DBS (LOC5576746), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021839981.1

ORF Insert Sequence:

ATGAAGTTCG GTAACTACAA CCTGACGACG GAAACGGCAA ACAGAAGGCA TCTCTTTCTT 
TTTCGCATCA AAAAAGTGAA GAGCACTGCA AGCTACATCG TTGGCAAAGT GCACCGGAAA 
ATCGGCACAG TGAAACGCAA AATGCACCAA AGGCACCAGC ATCGACACCA ACCGTCTCCA 
GTTCCGGCCA TTGCCGCCAT CCCAGATGGT TCCACCTCAG TCACCAGCAC CACTGCCACC 
AGCATCAGCC GTCGAAGGCC TCCGGGGTTG AACCGTTCCG AGTCTCCGTT GTCCTACCGA 
GCGTCCCTGT TTGACGAAAG GAACATCTGC GGCAGCGAAT TGAGTCGTTC GAACGTCAGT 
CGAACTAAGT TTTCACCTCA GGACTTTCCC ACCACATGGA TGGAAGGAGG CAACTCATTC 
GATCCATGTT TTGGTCAGGG AAAGATTGCC GGACAAGATG AAACGTTTCA GGTTTTTAGC 
AACAAAGCAC ATTTATTTCA CAAAAATAAA ACTATATCAG GAAGAAGGCC TCTGTTCGTG 
AAAAACTTTC AGCCTTTCAA TTCACTGCAA CGGGAAATCT CTTTGGCTAA AAAACAGCAC 
AATTTTGGCA GTTGTCCGGA AATCAACTCT GGCACATTGA AATCACAGAT TTCCCAGAAA 
TCTAAGCACT CACAAAAATC ACTGAAAAGT TTCAAATCAA ACAAGTCGTC CAGATATTGC 
AAAAGCAAGA TTGTCCAACT TTTGGAACGG AACAATGACG AAAAAACGGA AATGGATATT 
CTTCAGGAAT ACTTTGATAC GATGTCTTAT ACGGAAATCG TCAAAGATAA AGATTTCAAG 
AACTATCTTA TCAAGAAACA ATACATGGAC ATAATGGATT ACATATATAA CAGTTCCGGG 
TCGGAGTTGG GGAACTCAGA AATACGATCC ATTCCAGCGT CAATACAAGT ATCCAATAAC 
GATTGCGATT CGGTCAAAAA GGAAAAACTC ATGGCAGAAC TACCGAAGAA ATACTATTCG 
TACACCAATC CGCAAGTTTT CTGCAAGCAA GACCAGAACT TTGAGCGATA CTATCAAAAA 
TATGGCACAG TCCCACCGAA AGAATCGCAA GTATTCGGTT TCTCTTCGCT TCCCTCGCAG 
TACTACACCT TGGATCGTTT AGTTCAGACG CCGAAAGAGG ATATTCCTTG TTACGTAACA 
CTACCGAAGA AACCATCACG ATGTAAACGA ACTTATCAAC AGGTCCGTGA ATTTTGCGAA 
AAATCTCTTC TGAAGTATAC AAGCACGAAA CCAACCAGTG ATACATTGAA AATTCAGAAA 
GCTTCCAAGG AGTATACTGA AAAAGCCTAT AACCGAATTA TCAATGATTT CGTTCGAAAA 
CGAGGCTTCA CCAGCGTAGA CGAGTACCTT CAGTATTACT ACGGTAACTT CCTGAATCAG 
AAACCATTCC GCGTGAAGAA ACCGAACCAA TTCAATTCGC TTCCACCCCC GCCATACTCC 
AGTGGAGACT TCCATGAAAA TCACCTCCGA TCGCTAGAAT GCAAACCAGT TTCTCCTTCC 
CGAACCAGTG CAACCATAGC TAACGATTCG GGCGTAGATT GTAGTTCCAA CTTCACCAGT 
CTCATTTACT CCGATTCGTT CAAGAACTTT GACTACGTCA ACCTCACCAC CATGAATCGC 
AAAACCCGCA AGCGTTCTTC TTCGGCATGC TGTCACATGA ATGAGAAACC GCGCTTCTTC 
CTTCCGGTGG GAAGTGACGA CGAGGACATC GACGAAGAAC GGTTCACCTC GCATGTGCTT 
CTCGACGGCC TGATTGCCAC CAGCGACCAA ACTAATCTGC CCTATATCTT CGACGATATC 
GGGATCTATG AGAGTATCGA GTCGAACGAG AAGGAACGCT TCCGTGCCGA AGTGCGCTCC 
CGTAAGGATA GCCTGGTGGA GCGGAGTGGG GCACTGCTTC CGGCGTCAAC GAAAGCAGAG 
CCCGCCATCA CAGGAGGAGT TCGGCGAGGT GCGGATGCAG TGCCACCTCC AAAACCGAGC 
AGGCTGTTGA ACAAGGCAGT GGCGCCCGGG AAGAAACTGC TCAATGAACG GGCTACCGCG 
GGATGTGTTG CGCCGGAAAC TGCTCGGGAT GATGGCTACC GAGACGAGAT GCTGTCGGAG 
GAGATGAGTT CACTGGTGCC ACCAGGACTG CAGGGCAAGG CAGATATCTT GTTCGGAAAT 
CTGCACGAGC TGTACACCTT CCACAATGAC ATTTTCCTGA AGGATCTGGA GAACTGCATA 
TCCACCACCG AACTAGTCGC ATTGTGCTTC GTGCAGAGGC GGGACACATT CTTCCGGTTG 
TATTCGTACT ACTGTCAGAA CATTCCGCGC TCCGAACGGC TTCGAGAGAC CCTTGTGGAC 
ACGCATCTGT TCCTGCAGGA GTGCCAGAAG AAGCTCGGCC ACAAACTGCC CCTTGCTGCA 
TATTTGCTCA AACCAGTACA AAGGATCACC AAGTATCAAC TTCTCTTAAA GGATCTGTTA 
AAATTCAGTG ACACCGGAAC GTGTTCCCGG GAGCTGCAGA AGGCGTTGGA CTGTATGCTG 
GTGGTGCTCA AGTGCGTTAA CGATTCGATG CATCAGATTG CAATAACTGG TTTTCCCGCC 
GACCTCTCGC AGCAAGGGGA ACTTCTGCTG CAGGATTCGT TCCAAGTGTG GACTGAAAGT 
AAAAAAGATC TCCGGTTGCG ACTGAAAACC CAACATCGGC ACATTTTCCT CTACCAGAAG 
GCAATGCTTT TCTGCAAACA GGGTTCGAAA ACCGGCCACA ACAAGAGTAC CTACCAGTTC 
AAACACTGGC TACAGATGTC CCAAATCGGT CTAACGGAAT CGGTCCGAGG CGACCCACGG 
AGGTTCGAAG TGTGGCTCCA AGGCCGACAG GAAGTGCACA CGATACAGGC CAACACGGTG 
GAAATCAAGA ACAAGTGGGT GGCCGAGATC AAACGGGTGC TGTTGAACCA ACTGGAAGAA 
CTCAAGGGGG AGAAAATCAA ACAGTACGGA CTGAATCACA AACCCCTTCG TCACACGGCC 
TCGTGGGACC TGCCACCGTC GATACATGGG ACGCCCAACC GGACGCTGAG CTGTGACCAG 
CAGCTACCCC CACCGACCAA TGGCGGCATC GGAAACATTC AGCACCACCC AGCGATGGGC 
CACCAGCAGG ATGTCATCAC CCGCATTTCG CACCTGACCG AGGACAGCAT GATGAGTGCT 
ACGGGCATTT CCTCTTCCTC GTCCGAACAC GACAATCAGG AAACGAATGC CTGGAGCTCG 
GACTACTCGA ATAGTGAGGA CGAATTTACG CACGGGGAGG ATGGCGTACT GCCGGGTCAT 
AAGTTTGTAT CGCTAGCGGA CTATTGTGCG ATGGGAAACT CGGAGGTTTC CATGAAGGAA 
GGAGACATTG TGGAGCTGCT CAAAATCGGA TGTGCAGGGT GGTGGTTTGT GAAGGTCATC 
GGCAATAGTC TCGAAGGATG GGCCCCGGCA GCATACCTAG AGCCTATCAA CCGGAAGTCG 
TCCAGACTAC GTGGTGCTCG CAGCCAAGAC AAACTGAACG AACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5576746 ( XM_001662793.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001662843.1 Aedes aegypti AAEL012730-RA partial mRNA.

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CloneID	OAe11576
Clone ID Related Accession (Same CDS sequence)	XM_001662793.1
Accession Version	XM_001662793.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL012730-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001811499). COMPLETENESS: incomplete on the 5' end.

ATGATGCACC AAACCCGACG ACAACAGCAA CCTGCAGCCG GTCAGCAGCC TCCACCACCA 
CCAACGCCTG CTGCGACCGG CCCTGGACCT GGAGGAGGGG GCCCCGGGGG TGGCGGGACG 
GAGAATATTG CCAGGAGAGG GATTCCTATT TTTCGGAACA ATCGCAATGC TAACAGTTCC 
AGGACAAGTG CAATATCGGT AGGACCTAAG ATTGACACGG GACGGAAAGT TTCGGAATCG 
GTGCCTGGAA GAGGAAGTCA AGGTGAACTG GACCATCTCG ATGAGGACAT GTTCTCAAAG 
GTTCCCAAAA TGGAGTTGCT GAAGGTTAAT TATGTGAATT TGAAAAACGA TGGCGTTGCC 
GGAAGTGAGA AGAAGAAAGG GGCTGATGAT AAAAAGGTTG CCAAGGGAAC AGTGGCGGCA 
TCTGCGCCCA CTAAAGGTAA TGTGGCAAGT GCAATAAAAA AGCAGTTAAC CACCAGTAGC 
GTAAATTTGA CGAGCAGTGC GGGTAACGGG TCGACACCTA ACCTCAAAGA AGTCAAATCA 
AAGTATTTGG TTTACAAGAA GCATAACGGG TTTGGATCGA ACAATTCGAT TGCCGAAATA 
GGGGCAAAGC TGCAATACAT AAATGACACC GACGTGAAAT CCAAGAACGG GAAAAAGAAG 
TATGCTATAT TTGATAGCAA AAAGAAAAAC AAGTTGGAAA CAGTTCAGTA TTATTTCGAT 
AGTAGAAGTT ACGAGCGTTA TGTTGATAAC AAACTTTACG GAGTGGTTAA CAAAGATGGA 
CAGAAGCAAC CGCCACCTGG TGCACGGGAA ATGAGTCCAG CTCCTCACAA ACCAGATCCC 
AAACAGGAAT CAGTAGATCC CAATAAACGG CTGAGTTGGG ACTTGCGATC CAACCGACCA 
AGTATGCTCC GAGTGATAAA TCAATCAGGA AAGAAACCAT CGGAGACAAA TCTTCAGCCG 
TCACCATCAG TGAAAAGCCC CACCTTCAAG CAACAACCGC AACAGCTTCA TGATGCCCAT 
AACCGTTTCA AACTACAAAA GAGCCATACC AGTACCAATC TGATTACACG CCATAGATCA 
ATGAACGACA TCCATCGGAT AAATCAATTG TTCCTGGAAT CGAAAGGAAT CGGCGAATGT 
GCTTCCATCG GCGTTCATCC ACCACCTTCA CTTCCTTCAA TTCCTCCACT TCTATCGCGA 
CAACACAGTA GCAGTGTGAT CGCATTAAAT CGGAAGGTTG TGACCAAAAA TACAAACCCT 
GTAGTGATCG GAGCTCCAAC TGGTGTCGTG CGGGAAAACG TTCACCGGTT CGAGAAGAAT 
AAGGCCAAGA ATGACGAAAT GTCCGCAAAG CAGCAGAAAT ATTTGGTGCA CAAACGCCTG 
AGTGCTCGAT ACAAGGCCGA AGAAAGCGCA CTGTCGCTGG CAGCTGCAGC ATCAACGCCA 
TCCACACCTT CCCCCGCACC AGCTATAGGA TCCGACGATA CGATCAGTAA ACGGATCCGC 
AGGATCATCG ACGGTCATAA CGGTACCGGA TCGACGGAAA CGATCAATTT CTGCCCAGGA 
TCAACGACTA CGACGACGCA TCACCACAAA CAGCAAGAAC CAAAGCGGGA ACTGTGTAGA 
GTGCGGTCGA GGTCTTTGGA ACGGACACTC AGCGATGACG ACATGAGTAC ACTGAGTGCC 
AAGAGCAGTA AGACTACATG TGGATACAAA AACTGCAAGT TTTCCAACTG CCCTATGTCT 
TCGTCGTCTT CGTCCTCGTC TGCTTCAACG GTATCTAGTT CCTCCTGTGG ATCTAAAAAG 
TCTGCATCTG CCGAAAGCCA CAAAAGTTCT GGAAGCAACG GTAGCAAGTG CAAAGAGAAG 
GAACCGGAAA TCCTGTGCGG TAAGCGGACG TCGATCGTCA TCAACGACAG TGACAGCGAG 
ATCCTGTTCA ACAACGCTCT GAACGACAAG CTGAACAACA GCTGCGAGAA CGTGCGCGTG 
ATTTCATCCA AGATTAAAGA AATCGATTTG GAGACCAACC GGATGATTAT CGAGAAGTGC 
ATCGAGCCGA TAGCCGATAT TACCGAAACT GTATTAGCCA GGCAAAAGTT TTGGAATCAA 
CAGAATCAGA GGAATATCAA ACTAAACAAC AACCCGCAAA ACAAATCCTA TGACGACAAG 
GTCAACATAA ATAACAAAAT CAAGAACGAG GAGAACAACA GCATTAAAAT ATTTATTTCG 
AGTCAAGGAT CGGGCAAAAG TACAACCAGC ATAAGTGAGC CGGTATCATT GATTTCAACA 
TCAGCCAGCG GAAGTAGCGG AGGTGGGAGT TCGGCCGCTT CCACGGTGAG TTCCAATGGA 
GGAGATTCCG ATAAGGACGA TGGCTACTAC GATCAGAGTG AGAGGTCACT GAGTCCCGCT 
GACAACAACA AGCAGCCGTT ATCGATTGTT TCTACCGTAA GCACAGGAAG TACGGTTTGT 
AGTGAGGGAA CAAACATGTC AAGCGTAACG TGTAGCAACG GAAACTCTAA CGTGCATCGA 
TACACGTCCA AAACTAGCAT AAGGCTCGGG TGTGACGGAG CCTTGTTCTG GAACAACAAC 
TATTTCGAGG ACCTGGACAA CTGTCAATCG ATGGGTTCTG GGGATGGTGA TGGCGGAGGA 
GGAGGAGGAA ACGGTGGAGG ATGTTGCAAT GGAGCAGGGA GTTGTTGCTG CTGTTGTCGA 
CGGCGAAGAA GTATTGCACT GGGCAGCAAC AGTTGCATCA TTAGTTCGAT CGACATCGGT 
GGCTGCAATG GTTCTGTTGT CAGTGGCAAC GGATGCGCTA GTCATGGAAA TGGAAACGGA 
GTTAACGTCG GGGGCTGTAG TGGTGGGAGT GGGGCCTGCA ATGGAGGAGT AAGTGGCGGC 
GGCGGAGTGA TGCTGAGAAA AGCACAACTT TGCCAATGCG GAATGGCGAA CCACAACAGT 
AAGATTGAAA GCTTCTCGTC GAGGCCCCGC TCCATCGAGG GACCCCCGGA CTCTGGGATA 
TCCATCAGCA GCGATACCAT CATTGCCAGC TCGGACAGTG ATCTCAATCT GCAGAGTCAA 
ACGCAGGATT CGGACGAGCG GAAACTGAAA CGAGGTCACG TGCTTGCCGA GCTACTCGAG 
ACGGAACGGA TCTACGTGGC GGAAATGGGA TCAATTTTGA AGGGCTACCG AGACGAGATG 
CTGTCGGAGG AGATGAGTTC ACTGGTGCCA CCAGGACTGC AGGGCAAGGC AGATATCTTG 
TTCGGAAATC TGCACGAGCT GTACACCTTC CACAATGACA TTTTCCTGAA GGATCTGGAG 
AACTGCATAT CCACCACCGA ACTAGTCGCA TTGTGCTTCG TGCAGAGGCG GGACACATTC 
TTCCGGTTGT ATTCGTACTA CTGTCAGAAC ATTCCGCGCT CCGAACGGCT TCGAGAGACC 
CTTGTGGACA CGCATCTGTT CCTGCAGGAG TGCCAGAAGA AGCTCGGCCA CAAACTGCCC 
CTTGCTGCAT ATTTGCTCAA ACCAGTACAA AGGATCACCA AGTATCAACT TCTCTTAAAG 
GATCTGTTAA AATTCAGTGA CACCGGAACG TGTTCCCGGG AGCTGCAGAA GGCGTTGGAC 
TGTATGCTGG TGGTGCTCAA GTGCGTTAAC GATTCGATGC ATCAGATTGC AATAACTGGT 
TTTCCCGCCG ACCTCTCGCA GCAAGGGGAA CTTCTGCTGC AGGATTCGTT CCAAGTGTGG 
ACTGAAAGTA AAAAAGATCT CCGGTTGCGA CTGAAAACCC AACATCGGCA CATTTTCCTC 
TACCAGAAGG CAATGCTTTT CTGCAAACAG GGTTCGAAAA CCGGCCACAA CAAGAGTACC 
TACCAGTTCA AACACTGGCT ACAGATGTCC CAAATCGGTC TAACGGAATC GGTCCGAGGC 
GACCCACGGA GGTTCGAAGT GTGGCTCCAA GGCCGACAGG AAGTGCACAC GATACAGGCC 
AACACGGTGG AAATCAAGAA CAAGTGGGTG GCCGAGATCA AACGGGTGCT GTTGAACCAA 
CTGGAAGAAC TCAAGGGGGA GAAAATCAAA CAGTACGGAC TGAATCACAA ACCCCTTCGT 
CACACGGCCT CGTGGGACCT GCCACCGTCG ATACATGGGA CGCCCAACCG GACGCTGAGC 
TGTGACCAGC AGCTACCCCC ACCGACCAAT GGCGGCATCG GAAACATTCA GCACCACCCA 
GCGATGGGCC ACCAGCAGGA TGTCATCACC CGCATTTCGC ACCTGACCGA GGACAGCATG 
ATGAGTGCTA CGGGCATTTC CTCTTCCTCG TCCGAACACG ACAATCAGGA AACGAATGCC 
TGGAGCTCGG ACTACTCGAA TAGTGAGGAC GAATTTACGC ACGGGGAGGA TGGCGTACTG 
CCGGGTCATA AGTTTGTGTC GCTAGCGGAC TATTGTGCGA TGGGAAACTC GGAGGTTTCC 
ATGAAGGAAG GAGACATTGT GGAGCTGCTC AAAATCGGAT GTGCAGGGTG GTGGTTTGTG 
AAGGTCATCG GCAATAGTCT CGAAGGATGG GCCCCGGCAG CATACCTAGA GCCTATCAAC 
CGGAAGTCGT CCAGACTACG TGGTGCTCGC AGCCAAGACA AACTGAACGA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001662843.1
CDS	1..4617
Translation	MMHQTRRQQQPAAGQQPPPPPTPAATGPGPGGGGPGGGGTENIARRGIPIFRNNRNANSSRTSAISVGPKIDTGRKVSESVPGRGSQGELDHLDEDMFSKVPKMELLKVNYVNLKNDGVAGSEKKKGADDKKVAKGTVAASAPTKGNVASAIKKQLTTSSVNLTSSAGNGSTPNLKEVKSKYLVYKKHNGFGSNNSIAEIGAKLQYINDTDVKSKNGKKKYAIFDSKKKNKLETVQYYFDSRSYERYVDNKLYGVVNKDGQKQPPPGAREMSPAPHKPDPKQESVDPNKRLSWDLRSNRPSMLRVINQSGKKPSETNLQPSPSVKSPTFKQQPQQLHDAHNRFKLQKSHTSTNLITRHRSMNDIHRINQLFLESKGIGECASIGVHPPPSLPSIPPLLSRQHSSSVIALNRKVVTKNTNPVVIGAPTGVVRENVHRFEKNKAKNDEMSAKQQKYLVHKRLSARYKAEESALSLAAAASTPSTPSPAPAIGSDDTISKRIRRIIDGHNGTGSTETINFCPGSTTTTTHHHKQQEPKRELCRVRSRSLERTLSDDDMSTLSAKSSKTTCGYKNCKFSNCPMSSSSSSSSASTVSSSSCGSKKSASAESHKSSGSNGSKCKEKEPEILCGKRTSIVINDSDSEILFNNALNDKLNNSCENVRVISSKIKEIDLETNRMIIEKCIEPIADITETVLARQKFWNQQNQRNIKLNNNPQNKSYDDKVNINNKIKNEENNSIKIFISSQGSGKSTTSISEPVSLISTSASGSSGGGSSAASTVSSNGGDSDKDDGYYDQSERSLSPADNNKQPLSIVSTVSTGSTVCSEGTNMSSVTCSNGNSNVHRYTSKTSIRLGCDGALFWNNNYFEDLDNCQSMGSGDGDGGGGGGNGGGCCNGAGSCCCCCRRRRSIALGSNSCIISSIDIGGCNGSVVSGNGCASHGNGNGVNVGGCSGGSGACNGGVSGGGGVMLRKAQLCQCGMANHNSKIESFSSRPRSIEGPPDSGISISSDTIIASSDSDLNLQSQTQDSDERKLKRGHVLAELLETERIYVAEMGSILKGYRDEMLSEEMSSLVPPGLQGKADILFGNLHELYTFHNDIFLKDLENCISTTELVALCFVQRRDTFFRLYSYYCQNIPRSERLRETLVDTHLFLQECQKKLGHKLPLAAYLLKPVQRITKYQLLLKDLLKFSDTGTCSRELQKALDCMLVVLKCVNDSMHQIAITGFPADLSQQGELLLQDSFQVWTESKKDLRLRLKTQHRHIFLYQKAMLFCKQGSKTGHNKSTYQFKHWLQMSQIGLTESVRGDPRRFEVWLQGRQEVHTIQANTVEIKNKWVAEIKRVLLNQLEELKGEKIKQYGLNHKPLRHTASWDLPPSIHGTPNRTLSCDQQLPPPTNGGIGNIQHHPAMGHQQDVITRISHLTEDSMMSATGISSSSSEHDNQETNAWSSDYSNSEDEFTHGEDGVLPGHKFVSLADYCAMGNSEVSMKEGDIVELLKIGCAGWWFVKVIGNSLEGWAPAAYLEPINRKSSRLRGARSQDKLNEH

Target ORF information:

RefSeq Version	XM_001662793.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL012730-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001662793.1

ORF Insert Sequence:

ATGATGCACC AAACCCGACG ACAACAGCAA CCTGCAGCCG GTCAGCAGCC TCCACCACCA 
CCAACGCCTG CTGCGACCGG CCCTGGACCT GGAGGAGGGG GCCCCGGGGG TGGCGGGACG 
GAGAATATTG CCAGGAGAGG GATTCCTATT TTTCGGAACA ATCGCAATGC TAACAGTTCC 
AGGACAAGTG CAATATCGGT AGGACCTAAG ATTGACACGG GACGGAAAGT TTCGGAATCG 
GTGCCTGGAA GAGGAAGTCA AGGTGAACTG GACCATCTCG ATGAGGACAT GTTCTCAAAG 
GTTCCCAAAA TGGAGTTGCT GAAGGTTAAT TATGTGAATT TGAAAAACGA TGGCGTTGCC 
GGAAGTGAGA AGAAGAAAGG GGCTGATGAT AAAAAGGTTG CCAAGGGAAC AGTGGCGGCA 
TCTGCGCCCA CTAAAGGTAA TGTGGCAAGT GCAATAAAAA AGCAGTTAAC CACCAGTAGC 
GTAAATTTGA CGAGCAGTGC GGGTAACGGG TCGACACCTA ACCTCAAAGA AGTCAAATCA 
AAGTATTTGG TTTACAAGAA GCATAACGGG TTTGGATCGA ACAATTCGAT TGCCGAAATA 
GGGGCAAAGC TGCAATACAT AAATGACACC GACGTGAAAT CCAAGAACGG GAAAAAGAAG 
TATGCTATAT TTGATAGCAA AAAGAAAAAC AAGTTGGAAA CAGTTCAGTA TTATTTCGAT 
AGTAGAAGTT ACGAGCGTTA TGTTGATAAC AAACTTTACG GAGTGGTTAA CAAAGATGGA 
CAGAAGCAAC CGCCACCTGG TGCACGGGAA ATGAGTCCAG CTCCTCACAA ACCAGATCCC 
AAACAGGAAT CAGTAGATCC CAATAAACGG CTGAGTTGGG ACTTGCGATC CAACCGACCA 
AGTATGCTCC GAGTGATAAA TCAATCAGGA AAGAAACCAT CGGAGACAAA TCTTCAGCCG 
TCACCATCAG TGAAAAGCCC CACCTTCAAG CAACAACCGC AACAGCTTCA TGATGCCCAT 
AACCGTTTCA AACTACAAAA GAGCCATACC AGTACCAATC TGATTACACG CCATAGATCA 
ATGAACGACA TCCATCGGAT AAATCAATTG TTCCTGGAAT CGAAAGGAAT CGGCGAATGT 
GCTTCCATCG GCGTTCATCC ACCACCTTCA CTTCCTTCAA TTCCTCCACT TCTATCGCGA 
CAACACAGTA GCAGTGTGAT CGCATTAAAT CGGAAGGTTG TGACCAAAAA TACAAACCCT 
GTAGTGATCG GAGCTCCAAC TGGTGTCGTG CGGGAAAACG TTCACCGGTT CGAGAAGAAT 
AAGGCCAAGA ATGACGAAAT GTCCGCAAAG CAGCAGAAAT ATTTGGTGCA CAAACGCCTG 
AGTGCTCGAT ACAAGGCCGA AGAAAGCGCA CTGTCGCTGG CAGCTGCAGC ATCAACGCCA 
TCCACACCTT CCCCCGCACC AGCTATAGGA TCCGACGATA CGATCAGTAA ACGGATCCGC 
AGGATCATCG ACGGTCATAA CGGTACCGGA TCGACGGAAA CGATCAATTT CTGCCCAGGA 
TCAACGACTA CGACGACGCA TCACCACAAA CAGCAAGAAC CAAAGCGGGA ACTGTGTAGA 
GTGCGGTCGA GGTCTTTGGA ACGGACACTC AGCGATGACG ACATGAGTAC ACTGAGTGCC 
AAGAGCAGTA AGACTACATG TGGATACAAA AACTGCAAGT TTTCCAACTG CCCTATGTCT 
TCGTCGTCTT CGTCCTCGTC TGCTTCAACG GTATCTAGTT CCTCCTGTGG ATCTAAAAAG 
TCTGCATCTG CCGAAAGCCA CAAAAGTTCT GGAAGCAACG GTAGCAAGTG CAAAGAGAAG 
GAACCGGAAA TCCTGTGCGG TAAGCGGACG TCGATCGTCA TCAACGACAG TGACAGCGAG 
ATCCTGTTCA ACAACGCTCT GAACGACAAG CTGAACAACA GCTGCGAGAA CGTGCGCGTG 
ATTTCATCCA AGATTAAAGA AATCGATTTG GAGACCAACC GGATGATTAT CGAGAAGTGC 
ATCGAGCCGA TAGCCGATAT TACCGAAACT GTATTAGCCA GGCAAAAGTT TTGGAATCAA 
CAGAATCAGA GGAATATCAA ACTAAACAAC AACCCGCAAA ACAAATCCTA TGACGACAAG 
GTCAACATAA ATAACAAAAT CAAGAACGAG GAGAACAACA GCATTAAAAT ATTTATTTCG 
AGTCAAGGAT CGGGCAAAAG TACAACCAGC ATAAGTGAGC CGGTATCATT GATTTCAACA 
TCAGCCAGCG GAAGTAGCGG AGGTGGGAGT TCGGCCGCTT CCACGGTGAG TTCCAATGGA 
GGAGATTCCG ATAAGGACGA TGGCTACTAC GATCAGAGTG AGAGGTCACT GAGTCCCGCT 
GACAACAACA AGCAGCCGTT ATCGATTGTT TCTACCGTAA GCACAGGAAG TACGGTTTGT 
AGTGAGGGAA CAAACATGTC AAGCGTAACG TGTAGCAACG GAAACTCTAA CGTGCATCGA 
TACACGTCCA AAACTAGCAT AAGGCTCGGG TGTGACGGAG CCTTGTTCTG GAACAACAAC 
TATTTCGAGG ACCTGGACAA CTGTCAATCG ATGGGTTCTG GGGATGGTGA TGGCGGAGGA 
GGAGGAGGAA ACGGTGGAGG ATGTTGCAAT GGAGCAGGGA GTTGTTGCTG CTGTTGTCGA 
CGGCGAAGAA GTATTGCACT GGGCAGCAAC AGTTGCATCA TTAGTTCGAT CGACATCGGT 
GGCTGCAATG GTTCTGTTGT CAGTGGCAAC GGATGCGCTA GTCATGGAAA TGGAAACGGA 
GTTAACGTCG GGGGCTGTAG TGGTGGGAGT GGGGCCTGCA ATGGAGGAGT AAGTGGCGGC 
GGCGGAGTGA TGCTGAGAAA AGCACAACTT TGCCAATGCG GAATGGCGAA CCACAACAGT 
AAGATTGAAA GCTTCTCGTC GAGGCCCCGC TCCATCGAGG GACCCCCGGA CTCTGGGATA 
TCCATCAGCA GCGATACCAT CATTGCCAGC TCGGACAGTG ATCTCAATCT GCAGAGTCAA 
ACGCAGGATT CGGACGAGCG GAAACTGAAA CGAGGTCACG TGCTTGCCGA GCTACTCGAG 
ACGGAACGGA TCTACGTGGC GGAAATGGGA TCAATTTTGA AGGGCTACCG AGACGAGATG 
CTGTCGGAGG AGATGAGTTC ACTGGTGCCA CCAGGACTGC AGGGCAAGGC AGATATCTTG 
TTCGGAAATC TGCACGAGCT GTACACCTTC CACAATGACA TTTTCCTGAA GGATCTGGAG 
AACTGCATAT CCACCACCGA ACTAGTCGCA TTGTGCTTCG TGCAGAGGCG GGACACATTC 
TTCCGGTTGT ATTCGTACTA CTGTCAGAAC ATTCCGCGCT CCGAACGGCT TCGAGAGACC 
CTTGTGGACA CGCATCTGTT CCTGCAGGAG TGCCAGAAGA AGCTCGGCCA CAAACTGCCC 
CTTGCTGCAT ATTTGCTCAA ACCAGTACAA AGGATCACCA AGTATCAACT TCTCTTAAAG 
GATCTGTTAA AATTCAGTGA CACCGGAACG TGTTCCCGGG AGCTGCAGAA GGCGTTGGAC 
TGTATGCTGG TGGTGCTCAA GTGCGTTAAC GATTCGATGC ATCAGATTGC AATAACTGGT 
TTTCCCGCCG ACCTCTCGCA GCAAGGGGAA CTTCTGCTGC AGGATTCGTT CCAAGTGTGG 
ACTGAAAGTA AAAAAGATCT CCGGTTGCGA CTGAAAACCC AACATCGGCA CATTTTCCTC 
TACCAGAAGG CAATGCTTTT CTGCAAACAG GGTTCGAAAA CCGGCCACAA CAAGAGTACC 
TACCAGTTCA AACACTGGCT ACAGATGTCC CAAATCGGTC TAACGGAATC GGTCCGAGGC 
GACCCACGGA GGTTCGAAGT GTGGCTCCAA GGCCGACAGG AAGTGCACAC GATACAGGCC 
AACACGGTGG AAATCAAGAA CAAGTGGGTG GCCGAGATCA AACGGGTGCT GTTGAACCAA 
CTGGAAGAAC TCAAGGGGGA GAAAATCAAA CAGTACGGAC TGAATCACAA ACCCCTTCGT 
CACACGGCCT CGTGGGACCT GCCACCGTCG ATACATGGGA CGCCCAACCG GACGCTGAGC 
TGTGACCAGC AGCTACCCCC ACCGACCAAT GGCGGCATCG GAAACATTCA GCACCACCCA 
GCGATGGGCC ACCAGCAGGA TGTCATCACC CGCATTTCGC ACCTGACCGA GGACAGCATG 
ATGAGTGCTA CGGGCATTTC CTCTTCCTCG TCCGAACACG ACAATCAGGA AACGAATGCC 
TGGAGCTCGG ACTACTCGAA TAGTGAGGAC GAATTTACGC ACGGGGAGGA TGGCGTACTG 
CCGGGTCATA AGTTTGTGTC GCTAGCGGAC TATTGTGCGA TGGGAAACTC GGAGGTTTCC 
ATGAAGGAAG GAGACATTGT GGAGCTGCTC AAAATCGGAT GTGCAGGGTG GTGGTTTGTG 
AAGGTCATCG GCAATAGTCT CGAAGGATGG GCCCCGGCAG CATACCTAGA GCCTATCAAC 
CGGAAGTCGT CCAGACTACG TGGTGCTCGC AGCCAAGACA AACTGAACGA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5576746 ( XM_021839976.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021695668.1 Aedes aegypti guanine nucleotide exchange factor DBS (LOC5576746), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAe118513
Clone ID Related Accession (Same CDS sequence)	XM_021839976.1 , XM_021839977.1
Accession Version	XM_021839976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	uncharacterized protein LOC5576746 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCACC AAACCCGACG ACAACAGCAA CCTGCAGCCG GTCAGCAGCC TCCACCACCA 
CCAACGCCTG CTGCGACCGG CCCTGGACCT GGAGGAGGGG GCCCCGGGGG TGGCGGGACG 
GAGAATATTA CCAGGAGAGG AATTCCTATT TTTCGGAACA ATCGCAATGC TAACAGTTCC 
AGAACAAGTG CAATATCGGT AGGACCTAAG ATAGACACGG GACGGAAAGT TGCGGAATCG 
GTGCCCGGAA GAGGAAGTCA AGGTGAACTG GACCATCTCG ATGAGGACAT GTTCTCAAAG 
GTTCCCAAAA TGGAGTTACT GAAGGTTAAC TATGTGAATT TGAAAAACGA TGGCGTTGCC 
GGAAGTGAGA AGAAGAAGGG GGCTGATGAT AAAAAGGTTG CCAAGGGAAC GGTGGCAGCG 
TCTGCGCCCA CTAAAGGTAA TGTGGCAAGT GCAATTAAAA AGCAGTTAAC CACCAGTAGC 
GTGAATTTGA CGAGCAGTGC GGGTAACGGG TCGACACCTA ACCTCAAAGA AGTCAAATCA 
AAGTATTTGG TGTACAAGAA GCATAACGGG TTTGGATCGA ACAATTCGAT TGCCGAAATA 
GGGGCAAAGC TGCAATACAT AAATGATACC GACGTGAAAT CCAAGAACGG GAAGAAGAAG 
TACGCTATAT TCGATAGCAA AAAGAAGAAC AAGTTGGAAA CAGTTCAGTA TTATTTCGAT 
AGTAGAAGTT ACGAGCGTTA TGTTGATAAC AAACTTTACG GAGTGGTTAA CAAAGATGGA 
CAGAAGCAAC CGCCGCCTGG TGCACGAGAG ATGAGTCCAG CTCCTCACAA ACCAGATCCC 
AAACAGGAAT CAGTAGATCC CAATAAACGG CTGAGTTGGG ACTTGCGATC CAACCGACCA 
AGTATGCTCC GAGTGATAAA TCAATCAGGA AAGAAACCAT CGGAGACAAA TCTTCAGCCG 
TCACCATCAG TGAAAAGCCC CACCTTCAAG CAACAACCGC AACAGCTTCA TGATGCCCAT 
AACCGTTTCA AACTACAAAA GAGCCATACC AGTACCAATC TGATTACACG CCATAGATCA 
ATGAACGACA TCCATCGGAT AAATCAATTG TTCCTGGAAT CGAAAGGAAT CGGCGAAGGT 
GCTTCCATCG GCGTTCATCC ACCACCTTCA CTTCCTTCTA TTCCTCCACT TCTATCACGA 
CAGCACAGTA GCAGTGTGAT CGCATTAAAT CGCAGGGTTG TGACCAAAAA CACAAACCCT 
GTAGTGATCG GAGCTCCAAC TGGTGTCGTG CGGGAAAACG TTCACCGGTT CGAGAAGAAT 
AAGGCCAAGA ATGACGAAAT GTCCGCAAAG CAGCAGAAAT ATTTGGTGCA CAAACGCCTG 
AGTGCTCGAT ACAAGGCCGA AGAAAGCGCA CTGTCGCTGG CAGCTGCAGC ATCAACGCCA 
TCCACACCGT CCCCCGCACC AGCTATAGGA TCCGACGATA CGATCAGTAA ACGGATCCGC 
AGGATCATCG ACGGTCATAA CGGTACCGGA TCGACGGAAA CGATCAATTT CTGCCCAGGA 
TCAACGACTA CGACGACGCA TCACCACAAA CAGCAAGAAC CAAAGCGGGA ACTGTGTAGA 
GTGCGGTCGA AGTCTTTGGA ACGGACACTC AGCGATGACG ACATGAGTAC ACTGAGTGCC 
AAGAGCAGTA AGACTACATG TGGATACAAA AACTGCAAGT TTTCCAACTG CCCTATGTCT 
TCGTCGTCTT CCTCCTCGTC TGCTTCAACG GTATCTAGTT CCTCCTGTGG ATCTAAAAAG 
TCTGCATCTG CCGAAAGCCA CAAGAGTTCT GGAAGCAACG GCAGTAAGTG CAAAGAGAAG 
GAACCGGAAA TCCTGTGCGG TAAGCGGACG TCGATCGTCA TCAACGACAG TGACAGCGAG 
ATCCTGCTCA ACAACGCTCT GAACGACAAG CTGAACAACA GCGCCGAGAA CGTGCGCGTG 
ATTTCATCGA AGATTAAAGA GATAGATTTG GAAACCAATC GGATGATTAT CGAGAAGTGC 
ATCGAGCCGA TAGCCGATAT TACCGAAACT GTATTGGCCA GGCAAAAGTT TTGGAATCAA 
CAGAATCAGA GGAATATCAA ACTAAACAAC AACCCGCAAA ACAAATCCTA TGACGATAAG 
GTCAACATAA ACAACAAAAT CAAAAACGAG GAGAACAACA GCATCAAGAT TTTCATTTCG 
AGTCAAGGAT CGGGCAAAAG TACAACCAGC ATAAGTGAGC CGGTATCATT GATTTCAACA 
TCAGCCAGCG GAAGTAGCGG AGGCGGGAGT TCGGCCGCTT CCACGGTGAG TTCCAATGGA 
GGAGATTCCG ATAAGGACGA TGGCTACTAC GATCAGAGTG AGAGGTCACT GAGTCCCGCT 
GACAACAACA AGCAGCCGTT GTCGATTGTT TCCACCGTCA GCACAGGAAG TACGGTTTGC 
AGTGAGGGAA CAAACATGTC AAGTGTGACG TGTAGTAACG GAAACTCCAA TGTGCATCGA 
TACACGTCCA AAACTAGCAT TAGGCTCGGG TGTGACGGAG CCTTGTTTTG GAACAACAAC 
TATTTCGAGG ACCTGGACAA CTGTCAATCG ATGGGTTCTG GAGATGGTGA TGGCGGAGGA 
GGAGGAAACG GAGGAGGATG TTGCAATGGA GCAGGAAGTT GTTGCTGCTG TTGTCGACGG 
CGAAGAAGTA TTGCACTGGG CAGCAACAGT TGCATCATTA GTTCGATCGA CATCGGTGGC 
TGCAATGGTT CTGTTGTCAG TGGCAACGGA TGCGCTAGTC ATGGAAATGG AAACGGAGTT 
AACGTCGGGG GCTGTAGTGG TGGGAGTGGG GCCTGCAATG GAGGAGTAAG TGGCGGCGGC 
GGAGTGATGC TGAGAAAAGC ACAACTTTGC CAATGCGGAA TGGCGAACCA CAACAGTAAG 
ATTGAAAGCT TCTCGTCGAG GCCCCGCTCA ATCGAGGGAC CCCCGGACTC TGGGATATCC 
ATCAGCAGCG ATACCATCAT TGCCAGCTCG GACAGTGATC TCAATCTGCA GAGTCAAACG 
CAGGATTCGG ACGAGCGGAA ACTGAAACGA GGTCACGTGC TTGCCGAGCT ACTCGAGACG 
GAACGGATCT ACGTGGCGGA AATGGGATCA ATTTTGAAGG GCTACCGAGA CGAGATGCTG 
TCGGAGGAGA TGAGTTCACT GGTGCCACCA GGACTGCAGG GCAAGGCAGA TATCTTGTTC 
GGAAATCTGC ACGAGCTGTA CACCTTCCAC AATGACATTT TCCTGAAGGA TCTGGAGAAC 
TGCATATCCA CCACCGAACT AGTCGCATTG TGCTTCGTGC AGAGGCGGGA CACATTCTTC 
CGGTTGTATT CGTACTACTG TCAGAACATT CCGCGCTCCG AACGGCTTCG AGAGACCCTT 
GTGGACACGC ATCTGTTCCT GCAGGAGTGC CAGAAGAAGC TCGGCCACAA ACTGCCCCTT 
GCTGCATATT TGCTCAAACC AGTACAAAGG ATCACCAAGT ATCAACTTCT CTTAAAGGAT 
CTGTTAAAAT TCAGTGACAC CGGAACGTGT TCCCGGGAGC TGCAGAAGGC GTTGGACTGT 
ATGCTGGTGG TGCTCAAGTG CGTTAACGAT TCGATGCATC AGATTGCAAT AACTGGTTTT 
CCCGCCGACC TCTCGCAGCA AGGGGAACTT CTGCTGCAGG ATTCGTTCCA AGTGTGGACT 
GAAAGTAAAA AAGATCTCCG GTTGCGACTG AAAACCCAAC ATCGGCACAT TTTCCTCTAC 
CAGAAGGCAA TGCTTTTCTG CAAACAGGGT TCGAAAACCG GCCACAACAA GAGTACCTAC 
CAGTTCAAAC ACTGGCTACA GATGTCCCAA ATCGGTCTAA CGGAATCGGT CCGAGGCGAC 
CCACGGAGGT TCGAAGTGTG GCTCCAAGGC CGACAGGAAG TGCACACGAT ACAGGCCAAC 
ACGGTGGAAA TCAAGAACAA GTGGGTGGCC GAGATCAAAC GGGTGCTGTT GAACCAACTG 
GAAGAACTCA AGGGGGAGAA AATCAAACAG TACGGACTGA ATCACAAACC CCTTCGTCAC 
ACGGCCTCGT GGGACCTGCC ACCGTCGATA CATGGGACGC CCAACCGGAC GCTGAGCTGT 
GACCAGCAGC TACCCCCACC GACCAATGGC GGCATCGGAA ACATTCAGCA CCACCCAGCG 
ATGGGCCACC AGCAGGATGT CATCACCCGC ATTTCGCACC TGACCGAGGA CAGCATGATG 
AGTGCTACGG GCATTTCCTC TTCCTCGTCC GAACACGACA ATCAGGAAAC GAATGCCTGG 
AGCTCGGACT ACTCGAATAG TGAGGACGAA TTTACGCACG GGGAGGATGG CGTACTGCCG 
GGTCATAAGT TTGTATCGCT AGCGGACTAT TGTGCGATGG GAAACTCGGA GGTTTCCATG 
AAGGAAGGAG ACATTGTGGA GCTGCTCAAA ATCGGATGTG CAGGGTGGTG GTTTGTGAAG 
GTCATCGGCA ATAGTCTCGA AGGATGGGCC CCGGCAGCAT ACCTAGAGCC TATCAACCGG 
AAGTCGTCCA GACTACGTGG TGCTCGCAGC CAAGACAAAC TGAACGAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021695668.1
CDS	968..5581
Translation	MMHQTRRQQQPAAGQQPPPPPTPAATGPGPGGGGPGGGGTENITRRGIPIFRNNRNANSSRTSAISVGPKIDTGRKVAESVPGRGSQGELDHLDEDMFSKVPKMELLKVNYVNLKNDGVAGSEKKKGADDKKVAKGTVAASAPTKGNVASAIKKQLTTSSVNLTSSAGNGSTPNLKEVKSKYLVYKKHNGFGSNNSIAEIGAKLQYINDTDVKSKNGKKKYAIFDSKKKNKLETVQYYFDSRSYERYVDNKLYGVVNKDGQKQPPPGAREMSPAPHKPDPKQESVDPNKRLSWDLRSNRPSMLRVINQSGKKPSETNLQPSPSVKSPTFKQQPQQLHDAHNRFKLQKSHTSTNLITRHRSMNDIHRINQLFLESKGIGEGASIGVHPPPSLPSIPPLLSRQHSSSVIALNRRVVTKNTNPVVIGAPTGVVRENVHRFEKNKAKNDEMSAKQQKYLVHKRLSARYKAEESALSLAAAASTPSTPSPAPAIGSDDTISKRIRRIIDGHNGTGSTETINFCPGSTTTTTHHHKQQEPKRELCRVRSKSLERTLSDDDMSTLSAKSSKTTCGYKNCKFSNCPMSSSSSSSSASTVSSSSCGSKKSASAESHKSSGSNGSKCKEKEPEILCGKRTSIVINDSDSEILLNNALNDKLNNSAENVRVISSKIKEIDLETNRMIIEKCIEPIADITETVLARQKFWNQQNQRNIKLNNNPQNKSYDDKVNINNKIKNEENNSIKIFISSQGSGKSTTSISEPVSLISTSASGSSGGGSSAASTVSSNGGDSDKDDGYYDQSERSLSPADNNKQPLSIVSTVSTGSTVCSEGTNMSSVTCSNGNSNVHRYTSKTSIRLGCDGALFWNNNYFEDLDNCQSMGSGDGDGGGGGNGGGCCNGAGSCCCCCRRRRSIALGSNSCIISSIDIGGCNGSVVSGNGCASHGNGNGVNVGGCSGGSGACNGGVSGGGGVMLRKAQLCQCGMANHNSKIESFSSRPRSIEGPPDSGISISSDTIIASSDSDLNLQSQTQDSDERKLKRGHVLAELLETERIYVAEMGSILKGYRDEMLSEEMSSLVPPGLQGKADILFGNLHELYTFHNDIFLKDLENCISTTELVALCFVQRRDTFFRLYSYYCQNIPRSERLRETLVDTHLFLQECQKKLGHKLPLAAYLLKPVQRITKYQLLLKDLLKFSDTGTCSRELQKALDCMLVVLKCVNDSMHQIAITGFPADLSQQGELLLQDSFQVWTESKKDLRLRLKTQHRHIFLYQKAMLFCKQGSKTGHNKSTYQFKHWLQMSQIGLTESVRGDPRRFEVWLQGRQEVHTIQANTVEIKNKWVAEIKRVLLNQLEELKGEKIKQYGLNHKPLRHTASWDLPPSIHGTPNRTLSCDQQLPPPTNGGIGNIQHHPAMGHQQDVITRISHLTEDSMMSATGISSSSSEHDNQETNAWSSDYSNSEDEFTHGEDGVLPGHKFVSLADYCAMGNSEVSMKEGDIVELLKIGCAGWWFVKVIGNSLEGWAPAAYLEPINRKSSRLRGARSQDKLNEH

Target ORF information:

RefSeq Version	XM_021839976.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti guanine nucleotide exchange factor DBS (LOC5576746), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021839976.1

ORF Insert Sequence:

ATGATGCACC AAACCCGACG ACAACAGCAA CCTGCAGCCG GTCAGCAGCC TCCACCACCA 
CCAACGCCTG CTGCGACCGG CCCTGGACCT GGAGGAGGGG GCCCCGGGGG TGGCGGGACG 
GAGAATATTA CCAGGAGAGG AATTCCTATT TTTCGGAACA ATCGCAATGC TAACAGTTCC 
AGAACAAGTG CAATATCGGT AGGACCTAAG ATAGACACGG GACGGAAAGT TGCGGAATCG 
GTGCCCGGAA GAGGAAGTCA AGGTGAACTG GACCATCTCG ATGAGGACAT GTTCTCAAAG 
GTTCCCAAAA TGGAGTTACT GAAGGTTAAC TATGTGAATT TGAAAAACGA TGGCGTTGCC 
GGAAGTGAGA AGAAGAAGGG GGCTGATGAT AAAAAGGTTG CCAAGGGAAC GGTGGCAGCG 
TCTGCGCCCA CTAAAGGTAA TGTGGCAAGT GCAATTAAAA AGCAGTTAAC CACCAGTAGC 
GTGAATTTGA CGAGCAGTGC GGGTAACGGG TCGACACCTA ACCTCAAAGA AGTCAAATCA 
AAGTATTTGG TGTACAAGAA GCATAACGGG TTTGGATCGA ACAATTCGAT TGCCGAAATA 
GGGGCAAAGC TGCAATACAT AAATGATACC GACGTGAAAT CCAAGAACGG GAAGAAGAAG 
TACGCTATAT TCGATAGCAA AAAGAAGAAC AAGTTGGAAA CAGTTCAGTA TTATTTCGAT 
AGTAGAAGTT ACGAGCGTTA TGTTGATAAC AAACTTTACG GAGTGGTTAA CAAAGATGGA 
CAGAAGCAAC CGCCGCCTGG TGCACGAGAG ATGAGTCCAG CTCCTCACAA ACCAGATCCC 
AAACAGGAAT CAGTAGATCC CAATAAACGG CTGAGTTGGG ACTTGCGATC CAACCGACCA 
AGTATGCTCC GAGTGATAAA TCAATCAGGA AAGAAACCAT CGGAGACAAA TCTTCAGCCG 
TCACCATCAG TGAAAAGCCC CACCTTCAAG CAACAACCGC AACAGCTTCA TGATGCCCAT 
AACCGTTTCA AACTACAAAA GAGCCATACC AGTACCAATC TGATTACACG CCATAGATCA 
ATGAACGACA TCCATCGGAT AAATCAATTG TTCCTGGAAT CGAAAGGAAT CGGCGAAGGT 
GCTTCCATCG GCGTTCATCC ACCACCTTCA CTTCCTTCTA TTCCTCCACT TCTATCACGA 
CAGCACAGTA GCAGTGTGAT CGCATTAAAT CGCAGGGTTG TGACCAAAAA CACAAACCCT 
GTAGTGATCG GAGCTCCAAC TGGTGTCGTG CGGGAAAACG TTCACCGGTT CGAGAAGAAT 
AAGGCCAAGA ATGACGAAAT GTCCGCAAAG CAGCAGAAAT ATTTGGTGCA CAAACGCCTG 
AGTGCTCGAT ACAAGGCCGA AGAAAGCGCA CTGTCGCTGG CAGCTGCAGC ATCAACGCCA 
TCCACACCGT CCCCCGCACC AGCTATAGGA TCCGACGATA CGATCAGTAA ACGGATCCGC 
AGGATCATCG ACGGTCATAA CGGTACCGGA TCGACGGAAA CGATCAATTT CTGCCCAGGA 
TCAACGACTA CGACGACGCA TCACCACAAA CAGCAAGAAC CAAAGCGGGA ACTGTGTAGA 
GTGCGGTCGA AGTCTTTGGA ACGGACACTC AGCGATGACG ACATGAGTAC ACTGAGTGCC 
AAGAGCAGTA AGACTACATG TGGATACAAA AACTGCAAGT TTTCCAACTG CCCTATGTCT 
TCGTCGTCTT CCTCCTCGTC TGCTTCAACG GTATCTAGTT CCTCCTGTGG ATCTAAAAAG 
TCTGCATCTG CCGAAAGCCA CAAGAGTTCT GGAAGCAACG GCAGTAAGTG CAAAGAGAAG 
GAACCGGAAA TCCTGTGCGG TAAGCGGACG TCGATCGTCA TCAACGACAG TGACAGCGAG 
ATCCTGCTCA ACAACGCTCT GAACGACAAG CTGAACAACA GCGCCGAGAA CGTGCGCGTG 
ATTTCATCGA AGATTAAAGA GATAGATTTG GAAACCAATC GGATGATTAT CGAGAAGTGC 
ATCGAGCCGA TAGCCGATAT TACCGAAACT GTATTGGCCA GGCAAAAGTT TTGGAATCAA 
CAGAATCAGA GGAATATCAA ACTAAACAAC AACCCGCAAA ACAAATCCTA TGACGATAAG 
GTCAACATAA ACAACAAAAT CAAAAACGAG GAGAACAACA GCATCAAGAT TTTCATTTCG 
AGTCAAGGAT CGGGCAAAAG TACAACCAGC ATAAGTGAGC CGGTATCATT GATTTCAACA 
TCAGCCAGCG GAAGTAGCGG AGGCGGGAGT TCGGCCGCTT CCACGGTGAG TTCCAATGGA 
GGAGATTCCG ATAAGGACGA TGGCTACTAC GATCAGAGTG AGAGGTCACT GAGTCCCGCT 
GACAACAACA AGCAGCCGTT GTCGATTGTT TCCACCGTCA GCACAGGAAG TACGGTTTGC 
AGTGAGGGAA CAAACATGTC AAGTGTGACG TGTAGTAACG GAAACTCCAA TGTGCATCGA 
TACACGTCCA AAACTAGCAT TAGGCTCGGG TGTGACGGAG CCTTGTTTTG GAACAACAAC 
TATTTCGAGG ACCTGGACAA CTGTCAATCG ATGGGTTCTG GAGATGGTGA TGGCGGAGGA 
GGAGGAAACG GAGGAGGATG TTGCAATGGA GCAGGAAGTT GTTGCTGCTG TTGTCGACGG 
CGAAGAAGTA TTGCACTGGG CAGCAACAGT TGCATCATTA GTTCGATCGA CATCGGTGGC 
TGCAATGGTT CTGTTGTCAG TGGCAACGGA TGCGCTAGTC ATGGAAATGG AAACGGAGTT 
AACGTCGGGG GCTGTAGTGG TGGGAGTGGG GCCTGCAATG GAGGAGTAAG TGGCGGCGGC 
GGAGTGATGC TGAGAAAAGC ACAACTTTGC CAATGCGGAA TGGCGAACCA CAACAGTAAG 
ATTGAAAGCT TCTCGTCGAG GCCCCGCTCA ATCGAGGGAC CCCCGGACTC TGGGATATCC 
ATCAGCAGCG ATACCATCAT TGCCAGCTCG GACAGTGATC TCAATCTGCA GAGTCAAACG 
CAGGATTCGG ACGAGCGGAA ACTGAAACGA GGTCACGTGC TTGCCGAGCT ACTCGAGACG 
GAACGGATCT ACGTGGCGGA AATGGGATCA ATTTTGAAGG GCTACCGAGA CGAGATGCTG 
TCGGAGGAGA TGAGTTCACT GGTGCCACCA GGACTGCAGG GCAAGGCAGA TATCTTGTTC 
GGAAATCTGC ACGAGCTGTA CACCTTCCAC AATGACATTT TCCTGAAGGA TCTGGAGAAC 
TGCATATCCA CCACCGAACT AGTCGCATTG TGCTTCGTGC AGAGGCGGGA CACATTCTTC 
CGGTTGTATT CGTACTACTG TCAGAACATT CCGCGCTCCG AACGGCTTCG AGAGACCCTT 
GTGGACACGC ATCTGTTCCT GCAGGAGTGC CAGAAGAAGC TCGGCCACAA ACTGCCCCTT 
GCTGCATATT TGCTCAAACC AGTACAAAGG ATCACCAAGT ATCAACTTCT CTTAAAGGAT 
CTGTTAAAAT TCAGTGACAC CGGAACGTGT TCCCGGGAGC TGCAGAAGGC GTTGGACTGT 
ATGCTGGTGG TGCTCAAGTG CGTTAACGAT TCGATGCATC AGATTGCAAT AACTGGTTTT 
CCCGCCGACC TCTCGCAGCA AGGGGAACTT CTGCTGCAGG ATTCGTTCCA AGTGTGGACT 
GAAAGTAAAA AAGATCTCCG GTTGCGACTG AAAACCCAAC ATCGGCACAT TTTCCTCTAC 
CAGAAGGCAA TGCTTTTCTG CAAACAGGGT TCGAAAACCG GCCACAACAA GAGTACCTAC 
CAGTTCAAAC ACTGGCTACA GATGTCCCAA ATCGGTCTAA CGGAATCGGT CCGAGGCGAC 
CCACGGAGGT TCGAAGTGTG GCTCCAAGGC CGACAGGAAG TGCACACGAT ACAGGCCAAC 
ACGGTGGAAA TCAAGAACAA GTGGGTGGCC GAGATCAAAC GGGTGCTGTT GAACCAACTG 
GAAGAACTCA AGGGGGAGAA AATCAAACAG TACGGACTGA ATCACAAACC CCTTCGTCAC 
ACGGCCTCGT GGGACCTGCC ACCGTCGATA CATGGGACGC CCAACCGGAC GCTGAGCTGT 
GACCAGCAGC TACCCCCACC GACCAATGGC GGCATCGGAA ACATTCAGCA CCACCCAGCG 
ATGGGCCACC AGCAGGATGT CATCACCCGC ATTTCGCACC TGACCGAGGA CAGCATGATG 
AGTGCTACGG GCATTTCCTC TTCCTCGTCC GAACACGACA ATCAGGAAAC GAATGCCTGG 
AGCTCGGACT ACTCGAATAG TGAGGACGAA TTTACGCACG GGGAGGATGG CGTACTGCCG 
GGTCATAAGT TTGTATCGCT AGCGGACTAT TGTGCGATGG GAAACTCGGA GGTTTCCATG 
AAGGAAGGAG ACATTGTGGA GCTGCTCAAA ATCGGATGTG CAGGGTGGTG GTTTGTGAAG 
GTCATCGGCA ATAGTCTCGA AGGATGGGCC CCGGCAGCAT ACCTAGAGCC TATCAACCGG 
AAGTCGTCCA GACTACGTGG TGCTCGCAGC CAAGACAAAC TGAACGAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5576746 ( XM_021839977.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021695669.1 Aedes aegypti guanine nucleotide exchange factor DBS (LOC5576746), transcript variant X2, mRNA.

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CloneID	OAe118513
Clone ID Related Accession (Same CDS sequence)	XM_021839976.1 , XM_021839977.1
Accession Version	XM_021839977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	uncharacterized protein LOC5576746 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCACC AAACCCGACG ACAACAGCAA CCTGCAGCCG GTCAGCAGCC TCCACCACCA 
CCAACGCCTG CTGCGACCGG CCCTGGACCT GGAGGAGGGG GCCCCGGGGG TGGCGGGACG 
GAGAATATTA CCAGGAGAGG AATTCCTATT TTTCGGAACA ATCGCAATGC TAACAGTTCC 
AGAACAAGTG CAATATCGGT AGGACCTAAG ATAGACACGG GACGGAAAGT TGCGGAATCG 
GTGCCCGGAA GAGGAAGTCA AGGTGAACTG GACCATCTCG ATGAGGACAT GTTCTCAAAG 
GTTCCCAAAA TGGAGTTACT GAAGGTTAAC TATGTGAATT TGAAAAACGA TGGCGTTGCC 
GGAAGTGAGA AGAAGAAGGG GGCTGATGAT AAAAAGGTTG CCAAGGGAAC GGTGGCAGCG 
TCTGCGCCCA CTAAAGGTAA TGTGGCAAGT GCAATTAAAA AGCAGTTAAC CACCAGTAGC 
GTGAATTTGA CGAGCAGTGC GGGTAACGGG TCGACACCTA ACCTCAAAGA AGTCAAATCA 
AAGTATTTGG TGTACAAGAA GCATAACGGG TTTGGATCGA ACAATTCGAT TGCCGAAATA 
GGGGCAAAGC TGCAATACAT AAATGATACC GACGTGAAAT CCAAGAACGG GAAGAAGAAG 
TACGCTATAT TCGATAGCAA AAAGAAGAAC AAGTTGGAAA CAGTTCAGTA TTATTTCGAT 
AGTAGAAGTT ACGAGCGTTA TGTTGATAAC AAACTTTACG GAGTGGTTAA CAAAGATGGA 
CAGAAGCAAC CGCCGCCTGG TGCACGAGAG ATGAGTCCAG CTCCTCACAA ACCAGATCCC 
AAACAGGAAT CAGTAGATCC CAATAAACGG CTGAGTTGGG ACTTGCGATC CAACCGACCA 
AGTATGCTCC GAGTGATAAA TCAATCAGGA AAGAAACCAT CGGAGACAAA TCTTCAGCCG 
TCACCATCAG TGAAAAGCCC CACCTTCAAG CAACAACCGC AACAGCTTCA TGATGCCCAT 
AACCGTTTCA AACTACAAAA GAGCCATACC AGTACCAATC TGATTACACG CCATAGATCA 
ATGAACGACA TCCATCGGAT AAATCAATTG TTCCTGGAAT CGAAAGGAAT CGGCGAAGGT 
GCTTCCATCG GCGTTCATCC ACCACCTTCA CTTCCTTCTA TTCCTCCACT TCTATCACGA 
CAGCACAGTA GCAGTGTGAT CGCATTAAAT CGCAGGGTTG TGACCAAAAA CACAAACCCT 
GTAGTGATCG GAGCTCCAAC TGGTGTCGTG CGGGAAAACG TTCACCGGTT CGAGAAGAAT 
AAGGCCAAGA ATGACGAAAT GTCCGCAAAG CAGCAGAAAT ATTTGGTGCA CAAACGCCTG 
AGTGCTCGAT ACAAGGCCGA AGAAAGCGCA CTGTCGCTGG CAGCTGCAGC ATCAACGCCA 
TCCACACCGT CCCCCGCACC AGCTATAGGA TCCGACGATA CGATCAGTAA ACGGATCCGC 
AGGATCATCG ACGGTCATAA CGGTACCGGA TCGACGGAAA CGATCAATTT CTGCCCAGGA 
TCAACGACTA CGACGACGCA TCACCACAAA CAGCAAGAAC CAAAGCGGGA ACTGTGTAGA 
GTGCGGTCGA AGTCTTTGGA ACGGACACTC AGCGATGACG ACATGAGTAC ACTGAGTGCC 
AAGAGCAGTA AGACTACATG TGGATACAAA AACTGCAAGT TTTCCAACTG CCCTATGTCT 
TCGTCGTCTT CCTCCTCGTC TGCTTCAACG GTATCTAGTT CCTCCTGTGG ATCTAAAAAG 
TCTGCATCTG CCGAAAGCCA CAAGAGTTCT GGAAGCAACG GCAGTAAGTG CAAAGAGAAG 
GAACCGGAAA TCCTGTGCGG TAAGCGGACG TCGATCGTCA TCAACGACAG TGACAGCGAG 
ATCCTGCTCA ACAACGCTCT GAACGACAAG CTGAACAACA GCGCCGAGAA CGTGCGCGTG 
ATTTCATCGA AGATTAAAGA GATAGATTTG GAAACCAATC GGATGATTAT CGAGAAGTGC 
ATCGAGCCGA TAGCCGATAT TACCGAAACT GTATTGGCCA GGCAAAAGTT TTGGAATCAA 
CAGAATCAGA GGAATATCAA ACTAAACAAC AACCCGCAAA ACAAATCCTA TGACGATAAG 
GTCAACATAA ACAACAAAAT CAAAAACGAG GAGAACAACA GCATCAAGAT TTTCATTTCG 
AGTCAAGGAT CGGGCAAAAG TACAACCAGC ATAAGTGAGC CGGTATCATT GATTTCAACA 
TCAGCCAGCG GAAGTAGCGG AGGCGGGAGT TCGGCCGCTT CCACGGTGAG TTCCAATGGA 
GGAGATTCCG ATAAGGACGA TGGCTACTAC GATCAGAGTG AGAGGTCACT GAGTCCCGCT 
GACAACAACA AGCAGCCGTT GTCGATTGTT TCCACCGTCA GCACAGGAAG TACGGTTTGC 
AGTGAGGGAA CAAACATGTC AAGTGTGACG TGTAGTAACG GAAACTCCAA TGTGCATCGA 
TACACGTCCA AAACTAGCAT TAGGCTCGGG TGTGACGGAG CCTTGTTTTG GAACAACAAC 
TATTTCGAGG ACCTGGACAA CTGTCAATCG ATGGGTTCTG GAGATGGTGA TGGCGGAGGA 
GGAGGAAACG GAGGAGGATG TTGCAATGGA GCAGGAAGTT GTTGCTGCTG TTGTCGACGG 
CGAAGAAGTA TTGCACTGGG CAGCAACAGT TGCATCATTA GTTCGATCGA CATCGGTGGC 
TGCAATGGTT CTGTTGTCAG TGGCAACGGA TGCGCTAGTC ATGGAAATGG AAACGGAGTT 
AACGTCGGGG GCTGTAGTGG TGGGAGTGGG GCCTGCAATG GAGGAGTAAG TGGCGGCGGC 
GGAGTGATGC TGAGAAAAGC ACAACTTTGC CAATGCGGAA TGGCGAACCA CAACAGTAAG 
ATTGAAAGCT TCTCGTCGAG GCCCCGCTCA ATCGAGGGAC CCCCGGACTC TGGGATATCC 
ATCAGCAGCG ATACCATCAT TGCCAGCTCG GACAGTGATC TCAATCTGCA GAGTCAAACG 
CAGGATTCGG ACGAGCGGAA ACTGAAACGA GGTCACGTGC TTGCCGAGCT ACTCGAGACG 
GAACGGATCT ACGTGGCGGA AATGGGATCA ATTTTGAAGG GCTACCGAGA CGAGATGCTG 
TCGGAGGAGA TGAGTTCACT GGTGCCACCA GGACTGCAGG GCAAGGCAGA TATCTTGTTC 
GGAAATCTGC ACGAGCTGTA CACCTTCCAC AATGACATTT TCCTGAAGGA TCTGGAGAAC 
TGCATATCCA CCACCGAACT AGTCGCATTG TGCTTCGTGC AGAGGCGGGA CACATTCTTC 
CGGTTGTATT CGTACTACTG TCAGAACATT CCGCGCTCCG AACGGCTTCG AGAGACCCTT 
GTGGACACGC ATCTGTTCCT GCAGGAGTGC CAGAAGAAGC TCGGCCACAA ACTGCCCCTT 
GCTGCATATT TGCTCAAACC AGTACAAAGG ATCACCAAGT ATCAACTTCT CTTAAAGGAT 
CTGTTAAAAT TCAGTGACAC CGGAACGTGT TCCCGGGAGC TGCAGAAGGC GTTGGACTGT 
ATGCTGGTGG TGCTCAAGTG CGTTAACGAT TCGATGCATC AGATTGCAAT AACTGGTTTT 
CCCGCCGACC TCTCGCAGCA AGGGGAACTT CTGCTGCAGG ATTCGTTCCA AGTGTGGACT 
GAAAGTAAAA AAGATCTCCG GTTGCGACTG AAAACCCAAC ATCGGCACAT TTTCCTCTAC 
CAGAAGGCAA TGCTTTTCTG CAAACAGGGT TCGAAAACCG GCCACAACAA GAGTACCTAC 
CAGTTCAAAC ACTGGCTACA GATGTCCCAA ATCGGTCTAA CGGAATCGGT CCGAGGCGAC 
CCACGGAGGT TCGAAGTGTG GCTCCAAGGC CGACAGGAAG TGCACACGAT ACAGGCCAAC 
ACGGTGGAAA TCAAGAACAA GTGGGTGGCC GAGATCAAAC GGGTGCTGTT GAACCAACTG 
GAAGAACTCA AGGGGGAGAA AATCAAACAG TACGGACTGA ATCACAAACC CCTTCGTCAC 
ACGGCCTCGT GGGACCTGCC ACCGTCGATA CATGGGACGC CCAACCGGAC GCTGAGCTGT 
GACCAGCAGC TACCCCCACC GACCAATGGC GGCATCGGAA ACATTCAGCA CCACCCAGCG 
ATGGGCCACC AGCAGGATGT CATCACCCGC ATTTCGCACC TGACCGAGGA CAGCATGATG 
AGTGCTACGG GCATTTCCTC TTCCTCGTCC GAACACGACA ATCAGGAAAC GAATGCCTGG 
AGCTCGGACT ACTCGAATAG TGAGGACGAA TTTACGCACG GGGAGGATGG CGTACTGCCG 
GGTCATAAGT TTGTATCGCT AGCGGACTAT TGTGCGATGG GAAACTCGGA GGTTTCCATG 
AAGGAAGGAG ACATTGTGGA GCTGCTCAAA ATCGGATGTG CAGGGTGGTG GTTTGTGAAG 
GTCATCGGCA ATAGTCTCGA AGGATGGGCC CCGGCAGCAT ACCTAGAGCC TATCAACCGG 
AAGTCGTCCA GACTACGTGG TGCTCGCAGC CAAGACAAAC TGAACGAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021695669.1
CDS	810..5423
Translation	MMHQTRRQQQPAAGQQPPPPPTPAATGPGPGGGGPGGGGTENITRRGIPIFRNNRNANSSRTSAISVGPKIDTGRKVAESVPGRGSQGELDHLDEDMFSKVPKMELLKVNYVNLKNDGVAGSEKKKGADDKKVAKGTVAASAPTKGNVASAIKKQLTTSSVNLTSSAGNGSTPNLKEVKSKYLVYKKHNGFGSNNSIAEIGAKLQYINDTDVKSKNGKKKYAIFDSKKKNKLETVQYYFDSRSYERYVDNKLYGVVNKDGQKQPPPGAREMSPAPHKPDPKQESVDPNKRLSWDLRSNRPSMLRVINQSGKKPSETNLQPSPSVKSPTFKQQPQQLHDAHNRFKLQKSHTSTNLITRHRSMNDIHRINQLFLESKGIGEGASIGVHPPPSLPSIPPLLSRQHSSSVIALNRRVVTKNTNPVVIGAPTGVVRENVHRFEKNKAKNDEMSAKQQKYLVHKRLSARYKAEESALSLAAAASTPSTPSPAPAIGSDDTISKRIRRIIDGHNGTGSTETINFCPGSTTTTTHHHKQQEPKRELCRVRSKSLERTLSDDDMSTLSAKSSKTTCGYKNCKFSNCPMSSSSSSSSASTVSSSSCGSKKSASAESHKSSGSNGSKCKEKEPEILCGKRTSIVINDSDSEILLNNALNDKLNNSAENVRVISSKIKEIDLETNRMIIEKCIEPIADITETVLARQKFWNQQNQRNIKLNNNPQNKSYDDKVNINNKIKNEENNSIKIFISSQGSGKSTTSISEPVSLISTSASGSSGGGSSAASTVSSNGGDSDKDDGYYDQSERSLSPADNNKQPLSIVSTVSTGSTVCSEGTNMSSVTCSNGNSNVHRYTSKTSIRLGCDGALFWNNNYFEDLDNCQSMGSGDGDGGGGGNGGGCCNGAGSCCCCCRRRRSIALGSNSCIISSIDIGGCNGSVVSGNGCASHGNGNGVNVGGCSGGSGACNGGVSGGGGVMLRKAQLCQCGMANHNSKIESFSSRPRSIEGPPDSGISISSDTIIASSDSDLNLQSQTQDSDERKLKRGHVLAELLETERIYVAEMGSILKGYRDEMLSEEMSSLVPPGLQGKADILFGNLHELYTFHNDIFLKDLENCISTTELVALCFVQRRDTFFRLYSYYCQNIPRSERLRETLVDTHLFLQECQKKLGHKLPLAAYLLKPVQRITKYQLLLKDLLKFSDTGTCSRELQKALDCMLVVLKCVNDSMHQIAITGFPADLSQQGELLLQDSFQVWTESKKDLRLRLKTQHRHIFLYQKAMLFCKQGSKTGHNKSTYQFKHWLQMSQIGLTESVRGDPRRFEVWLQGRQEVHTIQANTVEIKNKWVAEIKRVLLNQLEELKGEKIKQYGLNHKPLRHTASWDLPPSIHGTPNRTLSCDQQLPPPTNGGIGNIQHHPAMGHQQDVITRISHLTEDSMMSATGISSSSSEHDNQETNAWSSDYSNSEDEFTHGEDGVLPGHKFVSLADYCAMGNSEVSMKEGDIVELLKIGCAGWWFVKVIGNSLEGWAPAAYLEPINRKSSRLRGARSQDKLNEH

Target ORF information:

RefSeq Version	XM_021839977.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti guanine nucleotide exchange factor DBS (LOC5576746), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021839977.1

ORF Insert Sequence:

ATGATGCACC AAACCCGACG ACAACAGCAA CCTGCAGCCG GTCAGCAGCC TCCACCACCA 
CCAACGCCTG CTGCGACCGG CCCTGGACCT GGAGGAGGGG GCCCCGGGGG TGGCGGGACG 
GAGAATATTA CCAGGAGAGG AATTCCTATT TTTCGGAACA ATCGCAATGC TAACAGTTCC 
AGAACAAGTG CAATATCGGT AGGACCTAAG ATAGACACGG GACGGAAAGT TGCGGAATCG 
GTGCCCGGAA GAGGAAGTCA AGGTGAACTG GACCATCTCG ATGAGGACAT GTTCTCAAAG 
GTTCCCAAAA TGGAGTTACT GAAGGTTAAC TATGTGAATT TGAAAAACGA TGGCGTTGCC 
GGAAGTGAGA AGAAGAAGGG GGCTGATGAT AAAAAGGTTG CCAAGGGAAC GGTGGCAGCG 
TCTGCGCCCA CTAAAGGTAA TGTGGCAAGT GCAATTAAAA AGCAGTTAAC CACCAGTAGC 
GTGAATTTGA CGAGCAGTGC GGGTAACGGG TCGACACCTA ACCTCAAAGA AGTCAAATCA 
AAGTATTTGG TGTACAAGAA GCATAACGGG TTTGGATCGA ACAATTCGAT TGCCGAAATA 
GGGGCAAAGC TGCAATACAT AAATGATACC GACGTGAAAT CCAAGAACGG GAAGAAGAAG 
TACGCTATAT TCGATAGCAA AAAGAAGAAC AAGTTGGAAA CAGTTCAGTA TTATTTCGAT 
AGTAGAAGTT ACGAGCGTTA TGTTGATAAC AAACTTTACG GAGTGGTTAA CAAAGATGGA 
CAGAAGCAAC CGCCGCCTGG TGCACGAGAG ATGAGTCCAG CTCCTCACAA ACCAGATCCC 
AAACAGGAAT CAGTAGATCC CAATAAACGG CTGAGTTGGG ACTTGCGATC CAACCGACCA 
AGTATGCTCC GAGTGATAAA TCAATCAGGA AAGAAACCAT CGGAGACAAA TCTTCAGCCG 
TCACCATCAG TGAAAAGCCC CACCTTCAAG CAACAACCGC AACAGCTTCA TGATGCCCAT 
AACCGTTTCA AACTACAAAA GAGCCATACC AGTACCAATC TGATTACACG CCATAGATCA 
ATGAACGACA TCCATCGGAT AAATCAATTG TTCCTGGAAT CGAAAGGAAT CGGCGAAGGT 
GCTTCCATCG GCGTTCATCC ACCACCTTCA CTTCCTTCTA TTCCTCCACT TCTATCACGA 
CAGCACAGTA GCAGTGTGAT CGCATTAAAT CGCAGGGTTG TGACCAAAAA CACAAACCCT 
GTAGTGATCG GAGCTCCAAC TGGTGTCGTG CGGGAAAACG TTCACCGGTT CGAGAAGAAT 
AAGGCCAAGA ATGACGAAAT GTCCGCAAAG CAGCAGAAAT ATTTGGTGCA CAAACGCCTG 
AGTGCTCGAT ACAAGGCCGA AGAAAGCGCA CTGTCGCTGG CAGCTGCAGC ATCAACGCCA 
TCCACACCGT CCCCCGCACC AGCTATAGGA TCCGACGATA CGATCAGTAA ACGGATCCGC 
AGGATCATCG ACGGTCATAA CGGTACCGGA TCGACGGAAA CGATCAATTT CTGCCCAGGA 
TCAACGACTA CGACGACGCA TCACCACAAA CAGCAAGAAC CAAAGCGGGA ACTGTGTAGA 
GTGCGGTCGA AGTCTTTGGA ACGGACACTC AGCGATGACG ACATGAGTAC ACTGAGTGCC 
AAGAGCAGTA AGACTACATG TGGATACAAA AACTGCAAGT TTTCCAACTG CCCTATGTCT 
TCGTCGTCTT CCTCCTCGTC TGCTTCAACG GTATCTAGTT CCTCCTGTGG ATCTAAAAAG 
TCTGCATCTG CCGAAAGCCA CAAGAGTTCT GGAAGCAACG GCAGTAAGTG CAAAGAGAAG 
GAACCGGAAA TCCTGTGCGG TAAGCGGACG TCGATCGTCA TCAACGACAG TGACAGCGAG 
ATCCTGCTCA ACAACGCTCT GAACGACAAG CTGAACAACA GCGCCGAGAA CGTGCGCGTG 
ATTTCATCGA AGATTAAAGA GATAGATTTG GAAACCAATC GGATGATTAT CGAGAAGTGC 
ATCGAGCCGA TAGCCGATAT TACCGAAACT GTATTGGCCA GGCAAAAGTT TTGGAATCAA 
CAGAATCAGA GGAATATCAA ACTAAACAAC AACCCGCAAA ACAAATCCTA TGACGATAAG 
GTCAACATAA ACAACAAAAT CAAAAACGAG GAGAACAACA GCATCAAGAT TTTCATTTCG 
AGTCAAGGAT CGGGCAAAAG TACAACCAGC ATAAGTGAGC CGGTATCATT GATTTCAACA 
TCAGCCAGCG GAAGTAGCGG AGGCGGGAGT TCGGCCGCTT CCACGGTGAG TTCCAATGGA 
GGAGATTCCG ATAAGGACGA TGGCTACTAC GATCAGAGTG AGAGGTCACT GAGTCCCGCT 
GACAACAACA AGCAGCCGTT GTCGATTGTT TCCACCGTCA GCACAGGAAG TACGGTTTGC 
AGTGAGGGAA CAAACATGTC AAGTGTGACG TGTAGTAACG GAAACTCCAA TGTGCATCGA 
TACACGTCCA AAACTAGCAT TAGGCTCGGG TGTGACGGAG CCTTGTTTTG GAACAACAAC 
TATTTCGAGG ACCTGGACAA CTGTCAATCG ATGGGTTCTG GAGATGGTGA TGGCGGAGGA 
GGAGGAAACG GAGGAGGATG TTGCAATGGA GCAGGAAGTT GTTGCTGCTG TTGTCGACGG 
CGAAGAAGTA TTGCACTGGG CAGCAACAGT TGCATCATTA GTTCGATCGA CATCGGTGGC 
TGCAATGGTT CTGTTGTCAG TGGCAACGGA TGCGCTAGTC ATGGAAATGG AAACGGAGTT 
AACGTCGGGG GCTGTAGTGG TGGGAGTGGG GCCTGCAATG GAGGAGTAAG TGGCGGCGGC 
GGAGTGATGC TGAGAAAAGC ACAACTTTGC CAATGCGGAA TGGCGAACCA CAACAGTAAG 
ATTGAAAGCT TCTCGTCGAG GCCCCGCTCA ATCGAGGGAC CCCCGGACTC TGGGATATCC 
ATCAGCAGCG ATACCATCAT TGCCAGCTCG GACAGTGATC TCAATCTGCA GAGTCAAACG 
CAGGATTCGG ACGAGCGGAA ACTGAAACGA GGTCACGTGC TTGCCGAGCT ACTCGAGACG 
GAACGGATCT ACGTGGCGGA AATGGGATCA ATTTTGAAGG GCTACCGAGA CGAGATGCTG 
TCGGAGGAGA TGAGTTCACT GGTGCCACCA GGACTGCAGG GCAAGGCAGA TATCTTGTTC 
GGAAATCTGC ACGAGCTGTA CACCTTCCAC AATGACATTT TCCTGAAGGA TCTGGAGAAC 
TGCATATCCA CCACCGAACT AGTCGCATTG TGCTTCGTGC AGAGGCGGGA CACATTCTTC 
CGGTTGTATT CGTACTACTG TCAGAACATT CCGCGCTCCG AACGGCTTCG AGAGACCCTT 
GTGGACACGC ATCTGTTCCT GCAGGAGTGC CAGAAGAAGC TCGGCCACAA ACTGCCCCTT 
GCTGCATATT TGCTCAAACC AGTACAAAGG ATCACCAAGT ATCAACTTCT CTTAAAGGAT 
CTGTTAAAAT TCAGTGACAC CGGAACGTGT TCCCGGGAGC TGCAGAAGGC GTTGGACTGT 
ATGCTGGTGG TGCTCAAGTG CGTTAACGAT TCGATGCATC AGATTGCAAT AACTGGTTTT 
CCCGCCGACC TCTCGCAGCA AGGGGAACTT CTGCTGCAGG ATTCGTTCCA AGTGTGGACT 
GAAAGTAAAA AAGATCTCCG GTTGCGACTG AAAACCCAAC ATCGGCACAT TTTCCTCTAC 
CAGAAGGCAA TGCTTTTCTG CAAACAGGGT TCGAAAACCG GCCACAACAA GAGTACCTAC 
CAGTTCAAAC ACTGGCTACA GATGTCCCAA ATCGGTCTAA CGGAATCGGT CCGAGGCGAC 
CCACGGAGGT TCGAAGTGTG GCTCCAAGGC CGACAGGAAG TGCACACGAT ACAGGCCAAC 
ACGGTGGAAA TCAAGAACAA GTGGGTGGCC GAGATCAAAC GGGTGCTGTT GAACCAACTG 
GAAGAACTCA AGGGGGAGAA AATCAAACAG TACGGACTGA ATCACAAACC CCTTCGTCAC 
ACGGCCTCGT GGGACCTGCC ACCGTCGATA CATGGGACGC CCAACCGGAC GCTGAGCTGT 
GACCAGCAGC TACCCCCACC GACCAATGGC GGCATCGGAA ACATTCAGCA CCACCCAGCG 
ATGGGCCACC AGCAGGATGT CATCACCCGC ATTTCGCACC TGACCGAGGA CAGCATGATG 
AGTGCTACGG GCATTTCCTC TTCCTCGTCC GAACACGACA ATCAGGAAAC GAATGCCTGG 
AGCTCGGACT ACTCGAATAG TGAGGACGAA TTTACGCACG GGGAGGATGG CGTACTGCCG 
GGTCATAAGT TTGTATCGCT AGCGGACTAT TGTGCGATGG GAAACTCGGA GGTTTCCATG 
AAGGAAGGAG ACATTGTGGA GCTGCTCAAA ATCGGATGTG CAGGGTGGTG GTTTGTGAAG 
GTCATCGGCA ATAGTCTCGA AGGATGGGCC CCGGCAGCAT ACCTAGAGCC TATCAACCGG 
AAGTCGTCCA GACTACGTGG TGCTCGCAGC CAAGACAAAC TGAACGAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5576746 ( XM_021839978.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021695670.1 Aedes aegypti guanine nucleotide exchange factor DBS (LOC5576746), transcript variant X3, mRNA.

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CloneID	OAe118514
Clone ID Related Accession (Same CDS sequence)	XM_021839978.1
Accession Version	XM_021839978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	uncharacterized protein LOC5576746 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCACC AAACCCGACG ACAACAGCAA CCTGCAGCCG GTCAGCAGCC TCCACCACCA 
CCAACGCCTG CTGCGACCGG CCCTGGACCT GGAGGAGGGG GCCCCGGGGG TGGCGGGACG 
GAGAATATTA CCAGGAGAGG AATTCCTATT TTTCGGAACA ATCGCAATGC TAACAGTTCC 
AGAACAAGTG CAATATCGGT AGGACCTAAG ATAGACACGG GACGGAAAGT TGCGGAATCG 
GTGCCCGGAA GAGGAAGTCA AGGTGAACTG GACCATCTCG ATGAGGACAT GTTCTCAAAG 
GTTCCCAAAA TGGAGTTACT GAAGGTTAAC TATGTGAATT TGAAAAACGA TGGCGTTGCC 
GGAAGTGAGA AGAAGAAGGG GGCTGATGAT AAAAAGGTTG CCAAGGGAAC GGTGGCAGCG 
TCTGCGCCCA CTAAAGGTAA TGTGGCAAGT GCAATTAAAA AGCAGTTAAC CACCAGTAGC 
GTGAATTTGA CGAGCAGTGC GGGTAACGGG TCGACACCTA ACCTCAAAGA AGTCAAATCA 
AAGTATTTGG TGTACAAGAA GCATAACGGG TTTGGATCGA ACAATTCGAT TGCCGAAATA 
GGGGCAAAGC TGCAATACAT AAATGATACC GACGTGAAAT CCAAGAACGG GAAGAAGAAG 
TACGCTATAT TCGATAGCAA AAAGAAGAAC AAGTTGGAAA CAGTTCAGTA TTATTTCGAT 
AGTAGAAGTT ACGAGCGTTA TGTTGATAAC AAACTTTACG GAGTGGTTAA CAAAGATGGA 
CAGAAGCAAC CGCCGCCTGG TGCACGAGAG ATGAGTCCAG CTCCTCACAA ACCAGATCCC 
AAACAGGAAT CAGTAGATCC CAATAAACGG CTGAGTTGGG ACTTGCGATC CAACCGACCA 
AGTATGCTCC GAGTGATAAA TCAATCAGGA AAGAAACCAT CGGAGACAAA TCTTCAGCCG 
TCACCATCAG TGAAAAGCCC CACCTTCAAG CAACAACCGC AACAGCTTCA TGATGCCCAT 
AACCGTTTCA AACTACAAAA GAGCCATACC AGTACCAATC TGATTACACG CCATAGATCA 
ATGAACGACA TCCATCGGAT AAATCAATTG TTCCTGGAAT CGAAAGGAAT CGGCGAAGGT 
GCTTCCATCG GCGTTCATCC ACCACCTTCA CTTCCTTCTA TTCCTCCACT TCTATCACGA 
CAGCACAGTA GCAGTGTGAT CGCATTAAAT CGCAGGGTTG TGACCAAAAA CACAAACCCT 
GTAGTGATCG GAGCTCCAAC TGGTGTCGTG CGGGAAAACG TTCACCGGTT CGAGAAGAAT 
AAGGCCAAGA ATGACGAAAT GTCCGCAAAG CAGCAGAAAT ATTTGGTGCA CAAACGCCTG 
AGTGCTCGAT ACAAGGCCGA AGAAAGCGCA CTGTCGCTGG CAGCTGCAGC ATCAACGCCA 
TCCACACCGT CCCCCGCACC AGCTATAGGA TCCGACGATA CGATCAGTAA ACGGATCCGC 
AGGATCATCG ACGGTCATAA CGGTACCGGA TCGACGGAAA CGATCAATTT CTGCCCAGGA 
TCAACGACTA CGACGACGCA TCACCACAAA CAGCAAGAAC CAAAGCGGGA ACTGTGTAGA 
GTGCGGTCGA AGTCTTTGGA ACGGACACTC AGCGATGACG ACATGAGTAC ACTGAGTGCC 
AAGAGCAGTA AGACTACATG TGGATACAAA AACTGCAAGT TTTCCAACTG CCCTATGTCT 
TCGTCGTCTT CCTCCTCGTC TGCTTCAACG GTATCTAGTT CCTCCTGTGG ATCTAAAAAG 
TCTGCATCTG CCGAAAGCCA CAAGAGTTCT GGAAGCAACG GCAGTAAGTG CAAAGAGAAG 
GAACCGGAAA TCCTGTGCGG TAAGCGGACG TCGATCGTCA TCAACGACAG TGACAGCGAG 
ATCCTGCTCA ACAACGCTCT GAACGACAAG CTGAACAACA GCGCCGAGAA CGTGCGCGTG 
ATTTCATCGA AGATTAAAGA GATAGATTTG GAAACCAATC GGATGATTAT CGAGAAGTGC 
ATCGAGCCGA TAGCCGATAT TACCGAAACT GTATTGGCCA GGCAAAAGTT TTGGAATCAA 
CAGAATCAGA GGAATATCAA ACTAAACAAC AACCCGCAAA ACAAATCCTA TGACGATAAG 
GTCAACATAA ACAACAAAAT CAAAAACGAG GAGAACAACA GCATCAAGAT TTTCATTTCG 
AGTCAAGGAT CGGGCAAAAG TACAACCAGC ATAAGTGAGC CGGTATCATT GATTTCAACA 
TCAGCCAGCG GAAGTAGCGG AGGCGGGAGT TCGGCCGCTT CCACGGTGAG TTCCAATGGA 
GGAGATTCCG ATAAGGACGA TGGCTACTAC GATCAGAGTG AGAGGTCACT GAGTCCCGCT 
GACAACAACA AGCAGCCGTT GTCGATTGTT TCCACCGTCA GCACAGGAAG TACGGTTTGC 
AGTGAGGGAA CAAACATGTC AAGTGTGACG TGTAGTAACG GAAACTCCAA TGTGCATCGA 
TACACGTCCA AAACTAGCAT TAGGCTCGGG TGTGACGGAG CCTTGTTTTG GAACAACAAC 
TATTTCGAGG ACCTGGACAA CTGTCAATCG ATGGGTTCTG GAGATGGTGA TGGCGGAGGA 
GGAGGAAACG GAGGAGGATG TTGCAATGGA GCAGGAAGTT GTTGCTGCTG TTGTCGACGG 
CGAAGAAGTA TTGCACTGGG CAGCAACAGT TGCATCATTA GTTCGATCGA CATCGGTGGC 
TGCAATGGTT CTGTTGTCAG TGGCAACGGA TGCGCTAGTC ATGGAAATGG AAACGGAGTT 
AACGTCGGGG GCTGTAGTGG TGGGAGTGGG GCCTGCAATG GAGGAGTAAG TGGCGGCGGC 
GGAGTGATGC TGAGAAAAGC ACAACTTTGC CAATGCGGAA TGGCGAACCA CAACAGTAAG 
ATTGAAAGCT TCTCGTCGAG GCCCCGCTCA ATCGAGGGAC CCCCGGACTC TGGGATATCC 
ATCAGCAGCG ATACCATCAT TGCCAGCTCG GACAGTGATC TCAATCTGCA GAGTCAAACG 
GATTCGGACG AGCGGAAACT GAAACGAGGT CACGTGCTTG CCGAGCTACT CGAGACGGAA 
CGGATCTACG TGGCGGAAAT GGGATCAATT TTGAAGGGCT ACCGAGACGA GATGCTGTCG 
GAGGAGATGA GTTCACTGGT GCCACCAGGA CTGCAGGGCA AGGCAGATAT CTTGTTCGGA 
AATCTGCACG AGCTGTACAC CTTCCACAAT GACATTTTCC TGAAGGATCT GGAGAACTGC 
ATATCCACCA CCGAACTAGT CGCATTGTGC TTCGTGCAGA GGCGGGACAC ATTCTTCCGG 
TTGTATTCGT ACTACTGTCA GAACATTCCG CGCTCCGAAC GGCTTCGAGA GACCCTTGTG 
GACACGCATC TGTTCCTGCA GGAGTGCCAG AAGAAGCTCG GCCACAAACT GCCCCTTGCT 
GCATATTTGC TCAAACCAGT ACAAAGGATC ACCAAGTATC AACTTCTCTT AAAGGATCTG 
TTAAAATTCA GTGACACCGG AACGTGTTCC CGGGAGCTGC AGAAGGCGTT GGACTGTATG 
CTGGTGGTGC TCAAGTGCGT TAACGATTCG ATGCATCAGA TTGCAATAAC TGGTTTTCCC 
GCCGACCTCT CGCAGCAAGG GGAACTTCTG CTGCAGGATT CGTTCCAAGT GTGGACTGAA 
AGTAAAAAAG ATCTCCGGTT GCGACTGAAA ACCCAACATC GGCACATTTT CCTCTACCAG 
AAGGCAATGC TTTTCTGCAA ACAGGGTTCG AAAACCGGCC ACAACAAGAG TACCTACCAG 
TTCAAACACT GGCTACAGAT GTCCCAAATC GGTCTAACGG AATCGGTCCG AGGCGACCCA 
CGGAGGTTCG AAGTGTGGCT CCAAGGCCGA CAGGAAGTGC ACACGATACA GGCCAACACG 
GTGGAAATCA AGAACAAGTG GGTGGCCGAG ATCAAACGGG TGCTGTTGAA CCAACTGGAA 
GAACTCAAGG GGGAGAAAAT CAAACAGTAC GGACTGAATC ACAAACCCCT TCGTCACACG 
GCCTCGTGGG ACCTGCCACC GTCGATACAT GGGACGCCCA ACCGGACGCT GAGCTGTGAC 
CAGCAGCTAC CCCCACCGAC CAATGGCGGC ATCGGAAACA TTCAGCACCA CCCAGCGATG 
GGCCACCAGC AGGATGTCAT CACCCGCATT TCGCACCTGA CCGAGGACAG CATGATGAGT 
GCTACGGGCA TTTCCTCTTC CTCGTCCGAA CACGACAATC AGGAAACGAA TGCCTGGAGC 
TCGGACTACT CGAATAGTGA GGACGAATTT ACGCACGGGG AGGATGGCGT ACTGCCGGGT 
CATAAGTTTG TATCGCTAGC GGACTATTGT GCGATGGGAA ACTCGGAGGT TTCCATGAAG 
GAAGGAGACA TTGTGGAGCT GCTCAAAATC GGATGTGCAG GGTGGTGGTT TGTGAAGGTC 
ATCGGCAATA GTCTCGAAGG ATGGGCCCCG GCAGCATACC TAGAGCCTAT CAACCGGAAG 
TCGTCCAGAC TACGTGGTGC TCGCAGCCAA GACAAACTGA ACGAACATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021695670.1
CDS	968..5578
Translation	MMHQTRRQQQPAAGQQPPPPPTPAATGPGPGGGGPGGGGTENITRRGIPIFRNNRNANSSRTSAISVGPKIDTGRKVAESVPGRGSQGELDHLDEDMFSKVPKMELLKVNYVNLKNDGVAGSEKKKGADDKKVAKGTVAASAPTKGNVASAIKKQLTTSSVNLTSSAGNGSTPNLKEVKSKYLVYKKHNGFGSNNSIAEIGAKLQYINDTDVKSKNGKKKYAIFDSKKKNKLETVQYYFDSRSYERYVDNKLYGVVNKDGQKQPPPGAREMSPAPHKPDPKQESVDPNKRLSWDLRSNRPSMLRVINQSGKKPSETNLQPSPSVKSPTFKQQPQQLHDAHNRFKLQKSHTSTNLITRHRSMNDIHRINQLFLESKGIGEGASIGVHPPPSLPSIPPLLSRQHSSSVIALNRRVVTKNTNPVVIGAPTGVVRENVHRFEKNKAKNDEMSAKQQKYLVHKRLSARYKAEESALSLAAAASTPSTPSPAPAIGSDDTISKRIRRIIDGHNGTGSTETINFCPGSTTTTTHHHKQQEPKRELCRVRSKSLERTLSDDDMSTLSAKSSKTTCGYKNCKFSNCPMSSSSSSSSASTVSSSSCGSKKSASAESHKSSGSNGSKCKEKEPEILCGKRTSIVINDSDSEILLNNALNDKLNNSAENVRVISSKIKEIDLETNRMIIEKCIEPIADITETVLARQKFWNQQNQRNIKLNNNPQNKSYDDKVNINNKIKNEENNSIKIFISSQGSGKSTTSISEPVSLISTSASGSSGGGSSAASTVSSNGGDSDKDDGYYDQSERSLSPADNNKQPLSIVSTVSTGSTVCSEGTNMSSVTCSNGNSNVHRYTSKTSIRLGCDGALFWNNNYFEDLDNCQSMGSGDGDGGGGGNGGGCCNGAGSCCCCCRRRRSIALGSNSCIISSIDIGGCNGSVVSGNGCASHGNGNGVNVGGCSGGSGACNGGVSGGGGVMLRKAQLCQCGMANHNSKIESFSSRPRSIEGPPDSGISISSDTIIASSDSDLNLQSQTDSDERKLKRGHVLAELLETERIYVAEMGSILKGYRDEMLSEEMSSLVPPGLQGKADILFGNLHELYTFHNDIFLKDLENCISTTELVALCFVQRRDTFFRLYSYYCQNIPRSERLRETLVDTHLFLQECQKKLGHKLPLAAYLLKPVQRITKYQLLLKDLLKFSDTGTCSRELQKALDCMLVVLKCVNDSMHQIAITGFPADLSQQGELLLQDSFQVWTESKKDLRLRLKTQHRHIFLYQKAMLFCKQGSKTGHNKSTYQFKHWLQMSQIGLTESVRGDPRRFEVWLQGRQEVHTIQANTVEIKNKWVAEIKRVLLNQLEELKGEKIKQYGLNHKPLRHTASWDLPPSIHGTPNRTLSCDQQLPPPTNGGIGNIQHHPAMGHQQDVITRISHLTEDSMMSATGISSSSSEHDNQETNAWSSDYSNSEDEFTHGEDGVLPGHKFVSLADYCAMGNSEVSMKEGDIVELLKIGCAGWWFVKVIGNSLEGWAPAAYLEPINRKSSRLRGARSQDKLNEH

Target ORF information:

RefSeq Version	XM_021839978.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti guanine nucleotide exchange factor DBS (LOC5576746), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021839978.1

ORF Insert Sequence:

ATGATGCACC AAACCCGACG ACAACAGCAA CCTGCAGCCG GTCAGCAGCC TCCACCACCA 
CCAACGCCTG CTGCGACCGG CCCTGGACCT GGAGGAGGGG GCCCCGGGGG TGGCGGGACG 
GAGAATATTA CCAGGAGAGG AATTCCTATT TTTCGGAACA ATCGCAATGC TAACAGTTCC 
AGAACAAGTG CAATATCGGT AGGACCTAAG ATAGACACGG GACGGAAAGT TGCGGAATCG 
GTGCCCGGAA GAGGAAGTCA AGGTGAACTG GACCATCTCG ATGAGGACAT GTTCTCAAAG 
GTTCCCAAAA TGGAGTTACT GAAGGTTAAC TATGTGAATT TGAAAAACGA TGGCGTTGCC 
GGAAGTGAGA AGAAGAAGGG GGCTGATGAT AAAAAGGTTG CCAAGGGAAC GGTGGCAGCG 
TCTGCGCCCA CTAAAGGTAA TGTGGCAAGT GCAATTAAAA AGCAGTTAAC CACCAGTAGC 
GTGAATTTGA CGAGCAGTGC GGGTAACGGG TCGACACCTA ACCTCAAAGA AGTCAAATCA 
AAGTATTTGG TGTACAAGAA GCATAACGGG TTTGGATCGA ACAATTCGAT TGCCGAAATA 
GGGGCAAAGC TGCAATACAT AAATGATACC GACGTGAAAT CCAAGAACGG GAAGAAGAAG 
TACGCTATAT TCGATAGCAA AAAGAAGAAC AAGTTGGAAA CAGTTCAGTA TTATTTCGAT 
AGTAGAAGTT ACGAGCGTTA TGTTGATAAC AAACTTTACG GAGTGGTTAA CAAAGATGGA 
CAGAAGCAAC CGCCGCCTGG TGCACGAGAG ATGAGTCCAG CTCCTCACAA ACCAGATCCC 
AAACAGGAAT CAGTAGATCC CAATAAACGG CTGAGTTGGG ACTTGCGATC CAACCGACCA 
AGTATGCTCC GAGTGATAAA TCAATCAGGA AAGAAACCAT CGGAGACAAA TCTTCAGCCG 
TCACCATCAG TGAAAAGCCC CACCTTCAAG CAACAACCGC AACAGCTTCA TGATGCCCAT 
AACCGTTTCA AACTACAAAA GAGCCATACC AGTACCAATC TGATTACACG CCATAGATCA 
ATGAACGACA TCCATCGGAT AAATCAATTG TTCCTGGAAT CGAAAGGAAT CGGCGAAGGT 
GCTTCCATCG GCGTTCATCC ACCACCTTCA CTTCCTTCTA TTCCTCCACT TCTATCACGA 
CAGCACAGTA GCAGTGTGAT CGCATTAAAT CGCAGGGTTG TGACCAAAAA CACAAACCCT 
GTAGTGATCG GAGCTCCAAC TGGTGTCGTG CGGGAAAACG TTCACCGGTT CGAGAAGAAT 
AAGGCCAAGA ATGACGAAAT GTCCGCAAAG CAGCAGAAAT ATTTGGTGCA CAAACGCCTG 
AGTGCTCGAT ACAAGGCCGA AGAAAGCGCA CTGTCGCTGG CAGCTGCAGC ATCAACGCCA 
TCCACACCGT CCCCCGCACC AGCTATAGGA TCCGACGATA CGATCAGTAA ACGGATCCGC 
AGGATCATCG ACGGTCATAA CGGTACCGGA TCGACGGAAA CGATCAATTT CTGCCCAGGA 
TCAACGACTA CGACGACGCA TCACCACAAA CAGCAAGAAC CAAAGCGGGA ACTGTGTAGA 
GTGCGGTCGA AGTCTTTGGA ACGGACACTC AGCGATGACG ACATGAGTAC ACTGAGTGCC 
AAGAGCAGTA AGACTACATG TGGATACAAA AACTGCAAGT TTTCCAACTG CCCTATGTCT 
TCGTCGTCTT CCTCCTCGTC TGCTTCAACG GTATCTAGTT CCTCCTGTGG ATCTAAAAAG 
TCTGCATCTG CCGAAAGCCA CAAGAGTTCT GGAAGCAACG GCAGTAAGTG CAAAGAGAAG 
GAACCGGAAA TCCTGTGCGG TAAGCGGACG TCGATCGTCA TCAACGACAG TGACAGCGAG 
ATCCTGCTCA ACAACGCTCT GAACGACAAG CTGAACAACA GCGCCGAGAA CGTGCGCGTG 
ATTTCATCGA AGATTAAAGA GATAGATTTG GAAACCAATC GGATGATTAT CGAGAAGTGC 
ATCGAGCCGA TAGCCGATAT TACCGAAACT GTATTGGCCA GGCAAAAGTT TTGGAATCAA 
CAGAATCAGA GGAATATCAA ACTAAACAAC AACCCGCAAA ACAAATCCTA TGACGATAAG 
GTCAACATAA ACAACAAAAT CAAAAACGAG GAGAACAACA GCATCAAGAT TTTCATTTCG 
AGTCAAGGAT CGGGCAAAAG TACAACCAGC ATAAGTGAGC CGGTATCATT GATTTCAACA 
TCAGCCAGCG GAAGTAGCGG AGGCGGGAGT TCGGCCGCTT CCACGGTGAG TTCCAATGGA 
GGAGATTCCG ATAAGGACGA TGGCTACTAC GATCAGAGTG AGAGGTCACT GAGTCCCGCT 
GACAACAACA AGCAGCCGTT GTCGATTGTT TCCACCGTCA GCACAGGAAG TACGGTTTGC 
AGTGAGGGAA CAAACATGTC AAGTGTGACG TGTAGTAACG GAAACTCCAA TGTGCATCGA 
TACACGTCCA AAACTAGCAT TAGGCTCGGG TGTGACGGAG CCTTGTTTTG GAACAACAAC 
TATTTCGAGG ACCTGGACAA CTGTCAATCG ATGGGTTCTG GAGATGGTGA TGGCGGAGGA 
GGAGGAAACG GAGGAGGATG TTGCAATGGA GCAGGAAGTT GTTGCTGCTG TTGTCGACGG 
CGAAGAAGTA TTGCACTGGG CAGCAACAGT TGCATCATTA GTTCGATCGA CATCGGTGGC 
TGCAATGGTT CTGTTGTCAG TGGCAACGGA TGCGCTAGTC ATGGAAATGG AAACGGAGTT 
AACGTCGGGG GCTGTAGTGG TGGGAGTGGG GCCTGCAATG GAGGAGTAAG TGGCGGCGGC 
GGAGTGATGC TGAGAAAAGC ACAACTTTGC CAATGCGGAA TGGCGAACCA CAACAGTAAG 
ATTGAAAGCT TCTCGTCGAG GCCCCGCTCA ATCGAGGGAC CCCCGGACTC TGGGATATCC 
ATCAGCAGCG ATACCATCAT TGCCAGCTCG GACAGTGATC TCAATCTGCA GAGTCAAACG 
GATTCGGACG AGCGGAAACT GAAACGAGGT CACGTGCTTG CCGAGCTACT CGAGACGGAA 
CGGATCTACG TGGCGGAAAT GGGATCAATT TTGAAGGGCT ACCGAGACGA GATGCTGTCG 
GAGGAGATGA GTTCACTGGT GCCACCAGGA CTGCAGGGCA AGGCAGATAT CTTGTTCGGA 
AATCTGCACG AGCTGTACAC CTTCCACAAT GACATTTTCC TGAAGGATCT GGAGAACTGC 
ATATCCACCA CCGAACTAGT CGCATTGTGC TTCGTGCAGA GGCGGGACAC ATTCTTCCGG 
TTGTATTCGT ACTACTGTCA GAACATTCCG CGCTCCGAAC GGCTTCGAGA GACCCTTGTG 
GACACGCATC TGTTCCTGCA GGAGTGCCAG AAGAAGCTCG GCCACAAACT GCCCCTTGCT 
GCATATTTGC TCAAACCAGT ACAAAGGATC ACCAAGTATC AACTTCTCTT AAAGGATCTG 
TTAAAATTCA GTGACACCGG AACGTGTTCC CGGGAGCTGC AGAAGGCGTT GGACTGTATG 
CTGGTGGTGC TCAAGTGCGT TAACGATTCG ATGCATCAGA TTGCAATAAC TGGTTTTCCC 
GCCGACCTCT CGCAGCAAGG GGAACTTCTG CTGCAGGATT CGTTCCAAGT GTGGACTGAA 
AGTAAAAAAG ATCTCCGGTT GCGACTGAAA ACCCAACATC GGCACATTTT CCTCTACCAG 
AAGGCAATGC TTTTCTGCAA ACAGGGTTCG AAAACCGGCC ACAACAAGAG TACCTACCAG 
TTCAAACACT GGCTACAGAT GTCCCAAATC GGTCTAACGG AATCGGTCCG AGGCGACCCA 
CGGAGGTTCG AAGTGTGGCT CCAAGGCCGA CAGGAAGTGC ACACGATACA GGCCAACACG 
GTGGAAATCA AGAACAAGTG GGTGGCCGAG ATCAAACGGG TGCTGTTGAA CCAACTGGAA 
GAACTCAAGG GGGAGAAAAT CAAACAGTAC GGACTGAATC ACAAACCCCT TCGTCACACG 
GCCTCGTGGG ACCTGCCACC GTCGATACAT GGGACGCCCA ACCGGACGCT GAGCTGTGAC 
CAGCAGCTAC CCCCACCGAC CAATGGCGGC ATCGGAAACA TTCAGCACCA CCCAGCGATG 
GGCCACCAGC AGGATGTCAT CACCCGCATT TCGCACCTGA CCGAGGACAG CATGATGAGT 
GCTACGGGCA TTTCCTCTTC CTCGTCCGAA CACGACAATC AGGAAACGAA TGCCTGGAGC 
TCGGACTACT CGAATAGTGA GGACGAATTT ACGCACGGGG AGGATGGCGT ACTGCCGGGT 
CATAAGTTTG TATCGCTAGC GGACTATTGT GCGATGGGAA ACTCGGAGGT TTCCATGAAG 
GAAGGAGACA TTGTGGAGCT GCTCAAAATC GGATGTGCAG GGTGGTGGTT TGTGAAGGTC 
ATCGGCAATA GTCTCGAAGG ATGGGCCCCG GCAGCATACC TAGAGCCTAT CAACCGGAAG 
TCGTCCAGAC TACGTGGTGC TCGCAGCCAA GACAAACTGA ACGAACATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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LOC5576746 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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