LOC5575001 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

The following LOC5575001 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC5575001 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAe10062 XM_001655425.1
Latest version!
Aedes aegypti AAEL002537-RA partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OAe131543 XM_021841867.1
Latest version!
Aedes aegypti uncharacterized LOC5575001 (LOC5575001), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OAe10062
    Clone ID Related Accession (Same CDS sequence) XM_001655425.1
    Accession Version XM_001655425.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1425398400000
    Organism Aedes aegypti(Yellow fever mosquito)
    Product AAEL002537-PA
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001811340). COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCGCATAA CGGCAGGCGA CGATCAGGTG GAGGTGGTCA AAGCCATCAG TCCCGATGAG 
    GTTGAAACGC GCGAGAACGA TCCGGACCAG GTGGCGCTGG AATCGGTAGA CCAGGACCAT
    CCGGGGGCGT CCTCTTCGGT GCACTCGTTC CCGCAGCACA TTCCGCTGTC GATCGGGCTG
    GGGCCGGGGC CGCAGTCGCA TGGACCGGGG CCGGCAGGAC CACATGGGCC GATCCTTGGC
    CATGGACCGA GCAGGGGTAT CCCTCACGGT CACGGTCAAC TGTATGGTCC TCCGCCGCCG
    CCGCCACCCC AACAACAGCA GCTCCCATCG GCTCACGCCT ATCATGGCCC ACCCCCACCG
    CTGCCACCGC CGAAACTTCA ACCGACCAAC GAGCTGGCCC CGGAGAAAAT CTACGGCGCT
    CCCCTCGCTG GGAACGACCT GTGGTCTTCT CCGCTCCAGG ACATGCCCAA GATCGTTTCG
    CTGGATGTGA AATGTGAAAA ACAAGGCATG AAGGTGTTCG TCCAGTTCGA CAAACCATTC
    TTCGGTATCA TCTTCTCCAA GGGCCACTAC AGTAACATCA ATTGTGTCCA TCTACCTGCT
    GGATTGGGTA AGAGTTCGGC TACCTTCGAC ATCGGAATTC ATGAGTGTGG AACCGCTGGA
    AATACCGATA ATGCACTGTA CAACGGCTAT TCAGGAACGG AGTCGGGTGC GGGATCTTAC
    TTCGAGAACA TCATTGTGCT GCAGTACGAT CCACAAGTGC AGGAAGTTTG GGATCAGGCC
    AGGAAACTGC GATGCACATG GCACGATCAG TACGAGAAGA GCGTAACGTT CCGGCCGTTC
    CCAGTAGATA TGTTGGATGT GATCAGAGCA GACTTTGCCG GAGATAACGT AGGCTGCTGG
    ATGCAGATTC AGGTTGGAAA GGGACCATGG GCTTCAGAGG TGTCTGGATT AGTGAAGATC
    GGCCAAACGA TGACGATGGT GTTGGCCATT AAGGATGACG ACAGCAAGTT TGATATGCTG
    GTGAGAAATT GCATGGCTCA TGACGGTAAA CGAGCTCCAA TTCAGTTGGT GGATCAACGT
    GGATGCGTGA CTCGACCGAA GCTTATGTCT CGATTTACGA AGATCAAGAA CTTTGGAGCA
    AGTGCTTCGG TACTGTCTTA CGCCCACTTC CAGGCTTTCA AGTTCCCTGA TTCGATGGAG
    GTTCACTTCC AATGTACGAT CCAAATCTGT CGTTACCAAT GCCCTGAGCA ATGTACCGAT
    CCAGGAATCT TGGATGTTCA TCACTTGTCC GGAGGACCTG AACATCAGCA GTACGGACCT
    CCACCACCGC CTCCTCAATC GCTCGACGCT CATGTATTTG CCAACCGTAA ACGAGACGAT
    CGCCGCATCC GTCGTACTCG TGCCGCCGGA GAACCGGAAG AGAAGGAAGA TGTCGGAATG
    AAACGTATTA TCCGGGTTGT GTCACCGGGA GATCTGACCT TCCCAATTGG CAGCCAGAAC
    AGCACCAACG ATTCGACCAT GATCTTCCCA GCTAGAGAAG AGGGACTAAT CTGTATGACG
    ACTCCTGGTT TTACGGTAAC GATCGTTGTC CTGTTGTCGA TCTTGCTTGC TTCGTGCGTA
    ACGTCTGCCT TCCTGTGTAT AAGACTGAAG CCGTTCTCGT TCTCATCGCG CAAGGAGCGA
    GCGGTTGCCT TCACTAATCC GCACCTCAGT CCGTCTACGG CCTCGATCGT GTCCAGCGGT
    CCAAGCTCAA TGGTCAGTGG TACGCTAAAG TCCACCAAAT CAGTCAAGTC GAGGAATTTC
    TACTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001655475.1
    CDS1..1809
    Translation

    Target ORF information:

    RefSeq Version XM_001655425.1
    Organism Aedes aegypti(Yellow fever mosquito)
    Definition Aedes aegypti AAEL002537-RA partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001655425.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCGCATAA CGGCAGGCGA CGATCAGGTG GAGGTGGTCA AAGCCATCAG TCCCGATGAG 
    GTTGAAACGC GCGAGAACGA TCCGGACCAG GTGGCGCTGG AATCGGTAGA CCAGGACCAT
    CCGGGGGCGT CCTCTTCGGT GCACTCGTTC CCGCAGCACA TTCCGCTGTC GATCGGGCTG
    GGGCCGGGGC CGCAGTCGCA TGGACCGGGG CCGGCAGGAC CACATGGGCC GATCCTTGGC
    CATGGACCGA GCAGGGGTAT CCCTCACGGT CACGGTCAAC TGTATGGTCC TCCGCCGCCG
    CCGCCACCCC AACAACAGCA GCTCCCATCG GCTCACGCCT ATCATGGCCC ACCCCCACCG
    CTGCCACCGC CGAAACTTCA ACCGACCAAC GAGCTGGCCC CGGAGAAAAT CTACGGCGCT
    CCCCTCGCTG GGAACGACCT GTGGTCTTCT CCGCTCCAGG ACATGCCCAA GATCGTTTCG
    CTGGATGTGA AATGTGAAAA ACAAGGCATG AAGGTGTTCG TCCAGTTCGA CAAACCATTC
    TTCGGTATCA TCTTCTCCAA GGGCCACTAC AGTAACATCA ATTGTGTCCA TCTACCTGCT
    GGATTGGGTA AGAGTTCGGC TACCTTCGAC ATCGGAATTC ATGAGTGTGG AACCGCTGGA
    AATACCGATA ATGCACTGTA CAACGGCTAT TCAGGAACGG AGTCGGGTGC GGGATCTTAC
    TTCGAGAACA TCATTGTGCT GCAGTACGAT CCACAAGTGC AGGAAGTTTG GGATCAGGCC
    AGGAAACTGC GATGCACATG GCACGATCAG TACGAGAAGA GCGTAACGTT CCGGCCGTTC
    CCAGTAGATA TGTTGGATGT GATCAGAGCA GACTTTGCCG GAGATAACGT AGGCTGCTGG
    ATGCAGATTC AGGTTGGAAA GGGACCATGG GCTTCAGAGG TGTCTGGATT AGTGAAGATC
    GGCCAAACGA TGACGATGGT GTTGGCCATT AAGGATGACG ACAGCAAGTT TGATATGCTG
    GTGAGAAATT GCATGGCTCA TGACGGTAAA CGAGCTCCAA TTCAGTTGGT GGATCAACGT
    GGATGCGTGA CTCGACCGAA GCTTATGTCT CGATTTACGA AGATCAAGAA CTTTGGAGCA
    AGTGCTTCGG TACTGTCTTA CGCCCACTTC CAGGCTTTCA AGTTCCCTGA TTCGATGGAG
    GTTCACTTCC AATGTACGAT CCAAATCTGT CGTTACCAAT GCCCTGAGCA ATGTACCGAT
    CCAGGAATCT TGGATGTTCA TCACTTGTCC GGAGGACCTG AACATCAGCA GTACGGACCT
    CCACCACCGC CTCCTCAATC GCTCGACGCT CATGTATTTG CCAACCGTAA ACGAGACGAT
    CGCCGCATCC GTCGTACTCG TGCCGCCGGA GAACCGGAAG AGAAGGAAGA TGTCGGAATG
    AAACGTATTA TCCGGGTTGT GTCACCGGGA GATCTGACCT TCCCAATTGG CAGCCAGAAC
    AGCACCAACG ATTCGACCAT GATCTTCCCA GCTAGAGAAG AGGGACTAAT CTGTATGACG
    ACTCCTGGTT TTACGGTAAC GATCGTTGTC CTGTTGTCGA TCTTGCTTGC TTCGTGCGTA
    ACGTCTGCCT TCCTGTGTAT AAGACTGAAG CCGTTCTCGT TCTCATCGCG CAAGGAGCGA
    GCGGTTGCCT TCACTAATCC GCACCTCAGT CCGTCTACGG CCTCGATCGT GTCCAGCGGT
    CCAAGCTCAA TGGTCAGTGG TACGCTAAAG TCCACCAAAT CAGTCAAGTC GAGGAATTTC
    TACTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OAe131543
    Clone ID Related Accession (Same CDS sequence) XM_021841867.1
    Accession Version XM_021841867.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1854bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1500393600000
    Organism Aedes aegypti(yellow fever mosquito)
    Product uncharacterized protein LOC5575001
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCGGTTGA CGAGGTTATT CTACACGACG TTACTAATTG GCATATTACA GATAACGGCA 
    GGCGACGATC AGGTGGAGGT GGTCAAAGCC ATCAGTCCCG ATGAGGTTGA AACGCGCGAG
    AACGATCCGG ACCAGGTGGC GCTGGAATCG GTAGACCAGG ACCATCCGGG GGCGTCCTCT
    TCGGTGCACT CGTTCCCGCA GCACATTCCG CTGTCGATCG GGCTGGGACC GGGGCCACAG
    TCGCATGGAC CGGGGCCGGC AGGGCCACAT GGGCCGATCC TTGGCCATGG ACCGAGCAGG
    GGTATCCCTC ACGGTCACGG TCAACTGTAT GGTCCTCCGC CGCCGCCGCC ACCCCAACAA
    CAGCAGCTCC CATCGGCTCA CGCCTATCAT GGCCCACCCC CACCGCTGCC ACCGCCGAAA
    CTTCAACCGA CCAACGAGCT GGCCCCGGAG AAACTCTACG GCGCTCCCCT CGCTGGGAAC
    GACCTGTGGT CTTCTCCGCT CCAGGACATG CCCAAGATCG TTTCGCTGGA TGTGAAATGT
    GAAAAGCAAG GCATGAAAGT GTTCGTCCAG TTCGACAAAC CATTCTTCGG TATCATCTTC
    TCCAAGGGCC ACTACAGTAA CATCAACTGC GTCCATCTGC CCGCCGGATT GGGTAAGAGT
    TCGGCTACCT TTGACATCGG AATTCATGAG TGTGGAACCG CTGGAAATAC CGATAATGCA
    CTGTACAACG GCTATTCAGG AACGGAGTCG GGTGCGGGAT CTTACTTCGA GAACATCATT
    GTGCTGCAGT ACGATCCACA AGTGCAGGAA GTTTGGGATC AAGCCAGGAA ACTGCGATGC
    ACATGGCACG ATCAGTACGA GAAGAGCGTA ACGTTCCGGC CGTTCCCAGT AGATATGTTG
    GATGTGATCA GAGCAGACTT TGCCGGAGAT AACGTAGGCT GCTGGATGCA GATCCAGGTT
    GGAAAGGGAC CATGGGCTTC AGAGGTGTCT GGATTGGTGA AGATCGGCCA AACGATGACG
    ATGGTGTTGG CCATTAAGGA TGACGACAGC AAGTTTGATA TGCTGGTGAG AAATTGCATG
    GCTCATGACG GTAAACGAGC TCCAATTCAG TTGGTGGATC AACGTGGATG CGTGACTCGT
    CCGAAGCTTA TGTCTCGGTT TACGAAGATC AAGAACTTTG GAGCAAGTGC TTCGGTACTG
    TCTTACGCCC ACTTCCAGGC TTTCAAGTTC CCTGATTCGA TGGAGGTTCA CTTCCAATGT
    ACGATCCAAA TCTGTCGTTA CCAATGCCCT GAGCAATGTA CCGATCCAGG AATCTTGGAT
    GTTCATCACT TGTCCGGAGG ACCTGAACAT CAGCAGTACG GACCTCCACC ACCACCTCCA
    CAATCACTTG ATGCTCACGT TTTTGCCAAC CGTAAACGAG ACGATCGCCG CATCCGTCGT
    ACTCGTGCCG CCGGAGAACC GGAAGAGAAG GAAGATGTCG GAATGAAGCG TATTATCCGG
    GTTGTGTCAC CGGGAGATCT GACCTTCCCA ATCGGCAGCC AGAACAGCAC CAACGATTCG
    ACCATGATCT TCCCAGCCAG AGAAGAGGGA CTAATCTGCA TGACGACTCC AGGTTTTACG
    GTAACGATCG TTGTCCTGTT GTCGATCTTG CTTGCTTCAT GCGTAACGTC TGCCTTCCTG
    TGTATAAGAC TGAAGCCGTT CTCGTTCTCA TCGCGCAAGG AGCGAGCGGT TGCCTTCACC
    AATCCGCACC TCAGTCCGTC TACGGCCTCG ATCGTGTCCA GCGGTTCAAG CTCAATGGTC
    AGTGGTACGC TAAAGTCCAC AAAATCAGTC AAGTCGAGGA ATTTCTACTC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021697559.1
    CDS715..2568
    Translation

    Target ORF information:

    RefSeq Version XM_021841867.1
    Organism Aedes aegypti(yellow fever mosquito)
    Definition Aedes aegypti uncharacterized LOC5575001 (LOC5575001), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021841867.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCGGTTGA CGAGGTTATT CTACACGACG TTACTAATTG GCATATTACA GATAACGGCA 
    GGCGACGATC AGGTGGAGGT GGTCAAAGCC ATCAGTCCCG ATGAGGTTGA AACGCGCGAG
    AACGATCCGG ACCAGGTGGC GCTGGAATCG GTAGACCAGG ACCATCCGGG GGCGTCCTCT
    TCGGTGCACT CGTTCCCGCA GCACATTCCG CTGTCGATCG GGCTGGGACC GGGGCCACAG
    TCGCATGGAC CGGGGCCGGC AGGGCCACAT GGGCCGATCC TTGGCCATGG ACCGAGCAGG
    GGTATCCCTC ACGGTCACGG TCAACTGTAT GGTCCTCCGC CGCCGCCGCC ACCCCAACAA
    CAGCAGCTCC CATCGGCTCA CGCCTATCAT GGCCCACCCC CACCGCTGCC ACCGCCGAAA
    CTTCAACCGA CCAACGAGCT GGCCCCGGAG AAACTCTACG GCGCTCCCCT CGCTGGGAAC
    GACCTGTGGT CTTCTCCGCT CCAGGACATG CCCAAGATCG TTTCGCTGGA TGTGAAATGT
    GAAAAGCAAG GCATGAAAGT GTTCGTCCAG TTCGACAAAC CATTCTTCGG TATCATCTTC
    TCCAAGGGCC ACTACAGTAA CATCAACTGC GTCCATCTGC CCGCCGGATT GGGTAAGAGT
    TCGGCTACCT TTGACATCGG AATTCATGAG TGTGGAACCG CTGGAAATAC CGATAATGCA
    CTGTACAACG GCTATTCAGG AACGGAGTCG GGTGCGGGAT CTTACTTCGA GAACATCATT
    GTGCTGCAGT ACGATCCACA AGTGCAGGAA GTTTGGGATC AAGCCAGGAA ACTGCGATGC
    ACATGGCACG ATCAGTACGA GAAGAGCGTA ACGTTCCGGC CGTTCCCAGT AGATATGTTG
    GATGTGATCA GAGCAGACTT TGCCGGAGAT AACGTAGGCT GCTGGATGCA GATCCAGGTT
    GGAAAGGGAC CATGGGCTTC AGAGGTGTCT GGATTGGTGA AGATCGGCCA AACGATGACG
    ATGGTGTTGG CCATTAAGGA TGACGACAGC AAGTTTGATA TGCTGGTGAG AAATTGCATG
    GCTCATGACG GTAAACGAGC TCCAATTCAG TTGGTGGATC AACGTGGATG CGTGACTCGT
    CCGAAGCTTA TGTCTCGGTT TACGAAGATC AAGAACTTTG GAGCAAGTGC TTCGGTACTG
    TCTTACGCCC ACTTCCAGGC TTTCAAGTTC CCTGATTCGA TGGAGGTTCA CTTCCAATGT
    ACGATCCAAA TCTGTCGTTA CCAATGCCCT GAGCAATGTA CCGATCCAGG AATCTTGGAT
    GTTCATCACT TGTCCGGAGG ACCTGAACAT CAGCAGTACG GACCTCCACC ACCACCTCCA
    CAATCACTTG ATGCTCACGT TTTTGCCAAC CGTAAACGAG ACGATCGCCG CATCCGTCGT
    ACTCGTGCCG CCGGAGAACC GGAAGAGAAG GAAGATGTCG GAATGAAGCG TATTATCCGG
    GTTGTGTCAC CGGGAGATCT GACCTTCCCA ATCGGCAGCC AGAACAGCAC CAACGATTCG
    ACCATGATCT TCCCAGCCAG AGAAGAGGGA CTAATCTGCA TGACGACTCC AGGTTTTACG
    GTAACGATCG TTGTCCTGTT GTCGATCTTG CTTGCTTCAT GCGTAACGTC TGCCTTCCTG
    TGTATAAGAC TGAAGCCGTT CTCGTTCTCA TCGCGCAAGG AGCGAGCGGT TGCCTTCACC
    AATCCGCACC TCAGTCCGTC TACGGCCTCG ATCGTGTCCA GCGGTTCAAG CTCAATGGTC
    AGTGGTACGC TAAAGTCCAC AAAATCAGTC AAGTCGAGGA ATTTCTACTC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Genome sequence of Aedes aegypti, a major arbovirus vector.
    Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
    Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW