AAEL_AAEL010598 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	AAEL_AAEL010598
Entrez Gene ID	5573561
Full Name	regulator of nonsense transcripts 2
Synonyms	AAEL010598
Gene Type	protein-coding
Organism	Aedes aegypti(Yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001660882.2	XP_001660932.2	regulator of nonsense transcripts 2
XM_001660882.1	XP_001660932.1	AAEL010598-PA

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AAEL_AAEL010598 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AAEL_AAEL010598 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAe08785	XM_001660882.1 Latest version!	Aedes aegypti AAEL010598-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAe132125	XM_001660882.2 Latest version!	Aedes aegypti regulator of nonsense transcripts 2 (LOC5573561), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AAEL_AAEL010598 ( XM_001660882.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_001660932.1 Aedes aegypti AAEL010598-RA partial mRNA.

Price & Availability↑

CloneID	OAe08785
Clone ID Related Accession (Same CDS sequence)	XM_001660882.1
Accession Version	XM_001660882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL010598-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001811228). COMPLETENESS: incomplete on the 5' end.

ATGGAATCCA CCATGAATTC CGATTGCGGA TCGGAACGGA AGAGTGAGGA AGTGGTGGAA 
AAGGAAGAAT CGCCTACCAA AGACTCGGAA GCTGCCAAGA AGGAGTTGGA CGATTATGTG 
GCGGAACTCA GCGGAAAATA CGAGCAGAAA TCTCAGCTGC GATCGCAGAA TGTAACTCCG 
GAACGTCCAC CGGAATCGTT CTTCACCAAA TGTGATTCAA GCTTGAAGAA AAATACGGCG 
TTTGTGAAGA AGTTGAAGCA ATTTACTGCC TCGCAGTTGG ATGGTTTGCT GAAGGATATG 
GCCGGGTTGA ATTTGACGAA GTACATTTCG GAAGTTAGTG CCGCGATTGT GGAAGCCAAA 
CTGAAGATGA GCGATGTTGT TCCGGCCCTG ATGTTGTGCA GCAAACTTCA TCAGACATAT 
GCGGAGTTCA GTCCGAGCTT CCTGGAGAAC TGGCAGAAGA TTTTGCACGT CAAAGCTGGT 
GAGAAAATTG GGAATCCGAG CAAGATGAGG GTGGACTTGA GATTCTACGC TGAACTGATC 
AGTGTTGGAA TCTTTAGCAA TAAAGTTGGA TTGCCTTTGC TGGGAGCTTG CTTAACAGCT 
TTGATATCCC AGGATAAGGA TGAGCATAGT AACTTGTCTA TAATCCTATC ATTCTGTCGA 
CATTGCGGCG ATGAGTATGC CGGATTAGTG CCCAAGAAGA TTATGGAACT AGCCAAGCAA 
TTCGAAGTGG CAATTCCTTC GTCAAATCTA CTCACCTCGG ATAAACAGCA AAACCTACGG 
AACCTACTGA AAGACTACTA TCAGACAGTC TGTGAGCATT TAACGAATGA GCACAAACAA 
CTACAATATG CGGAGAAATC TAGAAGGAGG ATGCTGGAAT CGAAAGGAGA GGTTCCAAAC 
GAAAAGAAAG AGCAAATCGA AGTGCTTCAG AACAGTTACG AAAAACTGTT AACGTCTGCC 
CAAACCATGG CCGATCTGCT GAATGAAACA CTACCGGAGC TCACCGTTGA AGCAGAAGCG 
TGTCCATCGG AAGGAAACGT AATTGATAGC ATTGGAGATG AGCAATTTGC CAATCTGGAT 
CCTTGGGGCG ATGAAGATAC TCGCAATTTC TACGTCAACC TACCGGATCT GCGACAGTTC 
CTGCCAAACT ACTGCGGAAA GAAAGAGGAA ATTATCCCGG ATGAACCTGC CATGACGGAG 
GAAGTGCTCG ATATGGAAGT TGAGCAAGAG CAAGACACCG AGGAAGTGGG CGATCCTCCC 
TCGGAGATAT CCGAAACTGC AGAACCAGAA GAAGAACCCA CGACCGCTCC CAGCACCCCA 
CATCACCATG CTAACCGGGA ACACTTCGAA GCATTTATTG CGAATCTGCA AAATTGTGTT 
AATACCGAAT TGATCGATTC GGCCGCGATC GAATTCCTGC TAAACCTAAA CACAAAAAAT 
AACCGGAAAA GGCTTGTAAA AGTTCTTTTC GGAGTGCAAA GAACACGGTT GGATCTCCTC 
CCCATGTACG CAAGATTAGT TGCTATCATC AACCTAGTTT CTGCGGATGT GGCGATAGAT 
CTGTGCCAGA TGTTGAAGGT GGACTTCAAG TATCAGATCA AGAAGAAGGA CCAAATCAAT 
ATCGAGACCA AGATCAAGGT GGTGCGGTAT ATCGGAGAGC TGGTGAAGTT TGGGCTGTAT 
AACAAAATTG AAGCGCTGTT TTGCCTGAAG TGCTTGCTGC ACAATTTCCA ACATCACCAT 
ATAGAGATGA CGTGCGCCTT CTTGGAAGTT TGCGGAGTTT ATCTGTATAA TTCGAAAGAC 
AGTCGCCTCC GAACAAATGT ATATCTCGAG CAAATGATGC GTCTCAAAAC CAACACTACG 
ATGGACAGTC GACATGCCCA GCAGGTGGAA ACAGCTTACT ATCTCGTTAA ACAACCGGAG 
GGAATGCGCA TCCAGCGCAA AGTTCGTCCA CTTTTCCACA CTTACATCCG GCATCTGATT 
TTTGAAGAGC TCAACAAGTC CAACGTTGAC AAAATGATAA AGCTGTTACG TCGATTGGAC 
TGGGAAGACC GTGACATTCA CGATTATGCA GTCAAGTGTC TTTCGAAGGC TTACAGCATC 
CGATATCACT TAATACGAAG TTTGGCCGAT TTGGTATCAG GATTGAGCTC ATATCAGGAG 
AAGGCCGTTG TAAAAGTGAT CGATGCAGTA TTTGAAGATA TCCGAGCAGG ACTGGAAATC 
CACGATACCA AACTCGCCCA GCGGAGGATT GCAATGGCCA AGTATCTTGG CGAGCTGTAT 
AACTATCGAT TGGTGGAATC GAAAAATGTT CTGAACACAC TTTACTCGAT AATATCACTC 
GGGGTCAGTG CCAGCCACGA TACACCATCG GAAGTGGACC CTCCAGGAAG TTTATTCAGG 
CTCAAGTTGG TTTGCGTTCT CCTGGATACC TGTGGACAGT ACTTCACCAG CGCGTCGAGC 
CGCAAGGGCT TGGACTACTT CCTGATATTC TTCCAAAATT ACTACTGGTT CAAAAAATCC 
GATCCTATTT TTACCAGCGA GACCAACAAG GACCTCTTCC CTATACTGGT TGATCATATG 
TATAAGGAAT GTTTCAAAAA TCTAAGACCA AAATTAAAGC TTTTAACCAG CTACGAGGCA 
TCCAAGGAAG CAATAGAGAA TATAGCGAAG AAAGTCTATC CCCAGTTGTT CAGCCCCCGT 
GACGATGACG AACGTAGTCT CAAAACCATC CAGGAGAACG ATACCGATAA GGATGACACC 
GTGACGGAAG ATTGCACATC GGAAGCGTTT GGGGATTCGG AAGACGACGA TGAAAATCCT 
CGAATTCAGA GAGGCGAGGA CGATGACGGC ATGGATGACG ACGTGGTTGA CGATGACGAC 
GAGGGTGATC TGAATCAAGA CCTCGATCCA CTGATAAAGC CTGCGCCAGT TGAAAAAACG 
ATCGAGGATT TGGAGTTCGA GAAAGAATTT GAACGGATGG CAACGGATAG CTTCTTGGAG 
CGCTTGAAGG AACCGGTGAA ACCCAATGTC AAGGACATTC CCGTTCCGAT TACAATGCGA 
AGCAGCGGGG CAAAGAAAAC ATACGAGCAA CTGCAGGAAA ATAAGGAAAA AACTGACACT 
GTTCCGTTCG TACTCATGGT CAGAGGTAAG GGCAAGCAAC AGCAGTACAA GTCATTTGAA 
GCACCATCGG ACAGTCAGCT GGCAATATAC GTCAAAGATC AGGAGCAGAA GATAAAGGAA 
GAAAACGATA GCGTTAAACG ACTAACGCTA AATATCACCG AACGACTGGA AGAGGAAGAT 
TACCAGGAGT CACTGCTCCA GAGTCAACGA TCGCCAGCAA GTACCAAAAT ACGGATACAA 
AAGCCTCCCA AATTCAAGCA TCCCAAGGGT GTCCCAGATG TTGATGCAAT TTTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001660932.1
CDS	1..3420
Translation	MESTMNSDCGSERKSEEVVEKEESPTKDSEAAKKELDDYVAELSGKYEQKSQLRSQNVTPERPPESFFTKCDSSLKKNTAFVKKLKQFTASQLDGLLKDMAGLNLTKYISEVSAAIVEAKLKMSDVVPALMLCSKLHQTYAEFSPSFLENWQKILHVKAGEKIGNPSKMRVDLRFYAELISVGIFSNKVGLPLLGACLTALISQDKDEHSNLSIILSFCRHCGDEYAGLVPKKIMELAKQFEVAIPSSNLLTSDKQQNLRNLLKDYYQTVCEHLTNEHKQLQYAEKSRRRMLESKGEVPNEKKEQIEVLQNSYEKLLTSAQTMADLLNETLPELTVEAEACPSEGNVIDSIGDEQFANLDPWGDEDTRNFYVNLPDLRQFLPNYCGKKEEIIPDEPAMTEEVLDMEVEQEQDTEEVGDPPSEISETAEPEEEPTTAPSTPHHHANREHFEAFIANLQNCVNTELIDSAAIEFLLNLNTKNNRKRLVKVLFGVQRTRLDLLPMYARLVAIINLVSADVAIDLCQMLKVDFKYQIKKKDQINIETKIKVVRYIGELVKFGLYNKIEALFCLKCLLHNFQHHHIEMTCAFLEVCGVYLYNSKDSRLRTNVYLEQMMRLKTNTTMDSRHAQQVETAYYLVKQPEGMRIQRKVRPLFHTYIRHLIFEELNKSNVDKMIKLLRRLDWEDRDIHDYAVKCLSKAYSIRYHLIRSLADLVSGLSSYQEKAVVKVIDAVFEDIRAGLEIHDTKLAQRRIAMAKYLGELYNYRLVESKNVLNTLYSIISLGVSASHDTPSEVDPPGSLFRLKLVCVLLDTCGQYFTSASSRKGLDYFLIFFQNYYWFKKSDPIFTSETNKDLFPILVDHMYKECFKNLRPKLKLLTSYEASKEAIENIAKKVYPQLFSPRDDDERSLKTIQENDTDKDDTVTEDCTSEAFGDSEDDDENPRIQRGEDDDGMDDDVVDDDDEGDLNQDLDPLIKPAPVEKTIEDLEFEKEFERMATDSFLERLKEPVKPNVKDIPVPITMRSSGAKKTYEQLQENKEKTDTVPFVLMVRGKGKQQQYKSFEAPSDSQLAIYVKDQEQKIKEENDSVKRLTLNITERLEEEDYQESLLQSQRSPASTKIRIQKPPKFKHPKGVPDVDAIFH

Target ORF information:

RefSeq Version	XM_001660882.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL010598-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001660882.1

ORF Insert Sequence:

ATGGAATCCA CCATGAATTC CGATTGCGGA TCGGAACGGA AGAGTGAGGA AGTGGTGGAA 
AAGGAAGAAT CGCCTACCAA AGACTCGGAA GCTGCCAAGA AGGAGTTGGA CGATTATGTG 
GCGGAACTCA GCGGAAAATA CGAGCAGAAA TCTCAGCTGC GATCGCAGAA TGTAACTCCG 
GAACGTCCAC CGGAATCGTT CTTCACCAAA TGTGATTCAA GCTTGAAGAA AAATACGGCG 
TTTGTGAAGA AGTTGAAGCA ATTTACTGCC TCGCAGTTGG ATGGTTTGCT GAAGGATATG 
GCCGGGTTGA ATTTGACGAA GTACATTTCG GAAGTTAGTG CCGCGATTGT GGAAGCCAAA 
CTGAAGATGA GCGATGTTGT TCCGGCCCTG ATGTTGTGCA GCAAACTTCA TCAGACATAT 
GCGGAGTTCA GTCCGAGCTT CCTGGAGAAC TGGCAGAAGA TTTTGCACGT CAAAGCTGGT 
GAGAAAATTG GGAATCCGAG CAAGATGAGG GTGGACTTGA GATTCTACGC TGAACTGATC 
AGTGTTGGAA TCTTTAGCAA TAAAGTTGGA TTGCCTTTGC TGGGAGCTTG CTTAACAGCT 
TTGATATCCC AGGATAAGGA TGAGCATAGT AACTTGTCTA TAATCCTATC ATTCTGTCGA 
CATTGCGGCG ATGAGTATGC CGGATTAGTG CCCAAGAAGA TTATGGAACT AGCCAAGCAA 
TTCGAAGTGG CAATTCCTTC GTCAAATCTA CTCACCTCGG ATAAACAGCA AAACCTACGG 
AACCTACTGA AAGACTACTA TCAGACAGTC TGTGAGCATT TAACGAATGA GCACAAACAA 
CTACAATATG CGGAGAAATC TAGAAGGAGG ATGCTGGAAT CGAAAGGAGA GGTTCCAAAC 
GAAAAGAAAG AGCAAATCGA AGTGCTTCAG AACAGTTACG AAAAACTGTT AACGTCTGCC 
CAAACCATGG CCGATCTGCT GAATGAAACA CTACCGGAGC TCACCGTTGA AGCAGAAGCG 
TGTCCATCGG AAGGAAACGT AATTGATAGC ATTGGAGATG AGCAATTTGC CAATCTGGAT 
CCTTGGGGCG ATGAAGATAC TCGCAATTTC TACGTCAACC TACCGGATCT GCGACAGTTC 
CTGCCAAACT ACTGCGGAAA GAAAGAGGAA ATTATCCCGG ATGAACCTGC CATGACGGAG 
GAAGTGCTCG ATATGGAAGT TGAGCAAGAG CAAGACACCG AGGAAGTGGG CGATCCTCCC 
TCGGAGATAT CCGAAACTGC AGAACCAGAA GAAGAACCCA CGACCGCTCC CAGCACCCCA 
CATCACCATG CTAACCGGGA ACACTTCGAA GCATTTATTG CGAATCTGCA AAATTGTGTT 
AATACCGAAT TGATCGATTC GGCCGCGATC GAATTCCTGC TAAACCTAAA CACAAAAAAT 
AACCGGAAAA GGCTTGTAAA AGTTCTTTTC GGAGTGCAAA GAACACGGTT GGATCTCCTC 
CCCATGTACG CAAGATTAGT TGCTATCATC AACCTAGTTT CTGCGGATGT GGCGATAGAT 
CTGTGCCAGA TGTTGAAGGT GGACTTCAAG TATCAGATCA AGAAGAAGGA CCAAATCAAT 
ATCGAGACCA AGATCAAGGT GGTGCGGTAT ATCGGAGAGC TGGTGAAGTT TGGGCTGTAT 
AACAAAATTG AAGCGCTGTT TTGCCTGAAG TGCTTGCTGC ACAATTTCCA ACATCACCAT 
ATAGAGATGA CGTGCGCCTT CTTGGAAGTT TGCGGAGTTT ATCTGTATAA TTCGAAAGAC 
AGTCGCCTCC GAACAAATGT ATATCTCGAG CAAATGATGC GTCTCAAAAC CAACACTACG 
ATGGACAGTC GACATGCCCA GCAGGTGGAA ACAGCTTACT ATCTCGTTAA ACAACCGGAG 
GGAATGCGCA TCCAGCGCAA AGTTCGTCCA CTTTTCCACA CTTACATCCG GCATCTGATT 
TTTGAAGAGC TCAACAAGTC CAACGTTGAC AAAATGATAA AGCTGTTACG TCGATTGGAC 
TGGGAAGACC GTGACATTCA CGATTATGCA GTCAAGTGTC TTTCGAAGGC TTACAGCATC 
CGATATCACT TAATACGAAG TTTGGCCGAT TTGGTATCAG GATTGAGCTC ATATCAGGAG 
AAGGCCGTTG TAAAAGTGAT CGATGCAGTA TTTGAAGATA TCCGAGCAGG ACTGGAAATC 
CACGATACCA AACTCGCCCA GCGGAGGATT GCAATGGCCA AGTATCTTGG CGAGCTGTAT 
AACTATCGAT TGGTGGAATC GAAAAATGTT CTGAACACAC TTTACTCGAT AATATCACTC 
GGGGTCAGTG CCAGCCACGA TACACCATCG GAAGTGGACC CTCCAGGAAG TTTATTCAGG 
CTCAAGTTGG TTTGCGTTCT CCTGGATACC TGTGGACAGT ACTTCACCAG CGCGTCGAGC 
CGCAAGGGCT TGGACTACTT CCTGATATTC TTCCAAAATT ACTACTGGTT CAAAAAATCC 
GATCCTATTT TTACCAGCGA GACCAACAAG GACCTCTTCC CTATACTGGT TGATCATATG 
TATAAGGAAT GTTTCAAAAA TCTAAGACCA AAATTAAAGC TTTTAACCAG CTACGAGGCA 
TCCAAGGAAG CAATAGAGAA TATAGCGAAG AAAGTCTATC CCCAGTTGTT CAGCCCCCGT 
GACGATGACG AACGTAGTCT CAAAACCATC CAGGAGAACG ATACCGATAA GGATGACACC 
GTGACGGAAG ATTGCACATC GGAAGCGTTT GGGGATTCGG AAGACGACGA TGAAAATCCT 
CGAATTCAGA GAGGCGAGGA CGATGACGGC ATGGATGACG ACGTGGTTGA CGATGACGAC 
GAGGGTGATC TGAATCAAGA CCTCGATCCA CTGATAAAGC CTGCGCCAGT TGAAAAAACG 
ATCGAGGATT TGGAGTTCGA GAAAGAATTT GAACGGATGG CAACGGATAG CTTCTTGGAG 
CGCTTGAAGG AACCGGTGAA ACCCAATGTC AAGGACATTC CCGTTCCGAT TACAATGCGA 
AGCAGCGGGG CAAAGAAAAC ATACGAGCAA CTGCAGGAAA ATAAGGAAAA AACTGACACT 
GTTCCGTTCG TACTCATGGT CAGAGGTAAG GGCAAGCAAC AGCAGTACAA GTCATTTGAA 
GCACCATCGG ACAGTCAGCT GGCAATATAC GTCAAAGATC AGGAGCAGAA GATAAAGGAA 
GAAAACGATA GCGTTAAACG ACTAACGCTA AATATCACCG AACGACTGGA AGAGGAAGAT 
TACCAGGAGT CACTGCTCCA GAGTCAACGA TCGCCAGCAA GTACCAAAAT ACGGATACAA 
AAGCCTCCCA AATTCAAGCA TCCCAAGGGT GTCCCAGATG TTGATGCAAT TTTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL010598 ( XM_001660882.2 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_001660932.2 Aedes aegypti regulator of nonsense transcripts 2 (LOC5573561), mRNA.

Price & Availability↑

CloneID	OAe132125
Clone ID Related Accession (Same CDS sequence)	XM_001660882.2
Accession Version	XM_001660882.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	regulator of nonsense transcripts 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 20, 2017 this sequence version replaced XM_001660882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCCA CCATGAATTC CGATTGCGGA TCGGAACGGA AGAGCGAGGA AGTGGTGGAA 
AAGGAAGAAT CGCCTACCAA AGACTCGGAA GCTGCCAAGA AGGAGTTGGA CGATTATGTG 
GCGGAACTCA GCGGAAAATA CGAGCAGAAA TCTCAGCTGC GATCGCAGAA TATTACTCCG 
GAACGTCCAC CGGAATCGTT CTTCACCAAA TGTGATTCAA GCTTGAAGAA AAATACGGCG 
TTTGTGAAGA AGTTGAAGCA ATTTACTGCC TCGCAGTTGG ATGGTTTGCT GAAGGATATG 
GCCGGGTTGA ATTTGACAAA GTACATTTCG GAAGTCAGTG CCGCGATTGT GGAAGCCAAA 
CTGAAGATGA GTGATGTGGT TCCGGCTCTG ATGTTATGCA GCAAACTTCA CCAGACCTAT 
GCGGAGTTCA GTCCGAGCTT TTTGGAGAAC TGGCAGAAGA TTTTGCACGT CAAAGCTGGT 
GAGAAAATTG GGAATCCGAG CAAGATGAGG GTGGACTTGA GATTCTACGC TGAACTGATC 
AGTGTTGGAA TCTTCAGTAA TAAGGTTGGA TTGCCTTTGC TGGGAGCATG CTTAACAGCT 
TTGATATCCC AGGATAAGGA CGAGCATAGT AATTTGTCCA TAATCCTATC ATTCTGTCGA 
CATTGTGGCG ATGAGTATGC TGGATTAGTG CCCAAGAAGA TTATGGAACT AGCCAAGCAA 
TTCGAAGTGG TTATTCCTTC GTCAAATCTA CTCACCTCGG ATAAACAGCA AAATCTACGG 
AACCTACTGA AAGACTACTA TCAGACAGTC TGTGAGCATT TAACGAACGA GCACAAACAA 
CTACAATATG CGGAGAAATC TAGAAGAAGG ATGCTGGAAT CGAAAGGAGA GGTTCCAAAC 
GAAAAGAAAG AGCAAATCGA AGTGCTTCAG AACAGTTACG AAAAACTGTT ATCGTCTGCC 
CAAACCATGG CCGATCTATT AAATGAAACA CTACCGGAAC TTACCGTTGA AGCAGAAGCG 
TGTCCATCGG AAGGAAACGT TATTGATAGC ATTGGAGATG AGCAATTTGC CAATCTGGAT 
CCTTGGGGCG ATGAAGATAC TCGCAATTTC TACGTCAATC TGCCGGATCT GCGACAGTTC 
CTGCCAAACT ACTGCGGAAA GAAAGAGGAA ATTATCCCGG ATGAACCTGC CATGACGGAG 
GAAGTGCTCG ATATGGAAGT TGAACAAGAG CAAGACACCG AGGAAGTGGG CGATCCTCCC 
TCGGAGATAT CCGAAACTGC GGAACCGGAA GAAGAACCCA CGACAGCTCC CAGCACCCCA 
CATCACCATG CTAACCGGGA ACACTTCGAA GCATTTATTG CGAATCTGCA AAATTGTGTT 
AATACCGAAT TGATCGATTC GGCCGCGATC GAATTCCTGC TAAACCTAAA CACAAAAAAT 
AACCGGAAAA GACTTGTGAA AGTTCTTTTT GGAGTGCAGA GAACGCGGTT GGATCTCCTC 
CCCATGTACG CAAGATTAGT TGCTATCATC AATCTGGTTT CGGCGGATGT GGCGATAGAT 
CTGTGCCAGA TGTTGAAGGT GGACTTCAAA TATCAGATCA AGAAGAAGGA TCAAATCAAT 
ATCGAGACCA AGATCAAAGT GGTGCGGTAC ATCGGAGAGC TCGTGAAGTT CGGACTGTAT 
AACAAAATTG AAGCGCTGTT CTGTTTGAAG TGCCTGCTAC ACAATTTCCA ACATCACCAC 
ATTGAAATGA CGTGCGCTTT TTTGGAAGTT TGCGGAGTTT ATCTGTACAA TTCGAAAGAC 
AGTCGGCTTC GAACCAATGT ATATCTCGAG CAAATGATGC GTCTCAAAAC CAACACTACG 
ATGGACAGTC GACACGCCCA GCAGGTGGAA ACAGCTTACT ATCTCGTCAA ACAACCGGAG 
GGAATGCGCA TCCAGCGCAA AGTTCGTCCA CTTTTCCACA CCTACATCCG ACATCTGATT 
TTTGAAGAGC TCAACAAGTC CAACGTAGAC AAAATGATTA AACTGTTGCG TCGCTTGGAC 
TGGGAAGATC GTGACATTCA CGACTATGCA GTCAAGTGTC TTTCGAAGGC TTACAGCATT 
CGGTATCACT TAATACGAAG TTTGGCCGAT TTGGTATCAG GATTGAGCTC ATATCAGGAA 
AAGGCCGTTG TAAAAGTGAT CGATGCAGTA TTTGAAGATA TCCGTGCGGG ACTAGAAATC 
CACGACACCA AACTCGCCCA GCGAAGGATT GCAATGGCTA AGTATCTTGG CGAGCTGTAT 
AACTATCGAT TGGTGGAATC GAAAAATGTT CTGAACACAC TATACTCGAT AATATCACTC 
GGGGTCAGTG CCAGCCACGA TACACCATCG GAAGTGGATC CTCCAGGAAG TTTATTCAGG 
CTCAAGCTGG TTTGCGTTCT CCTGGATACA TGTGGACAGT ACTTTACCAG CGCGTCGAGC 
CGCAAGGGCT TGGACTACTT CCTGATATTC TTCCAAAATT ACTACTGGTT CAAAAAATCC 
GATCCTATTT TTACCAGCGA GACGAACAAG GACCTCTTCC CTATACTGGT TGATCATATG 
TATAAGGAAT GTTTTAAAAA TCTACGACCA AAACTAAAGC TATTAACCAG CTACGAGGCA 
TCCAAGGAAG CAATAGAGAA TATAGCGAAG AAAGTCTATC CCCAGTTGTT CAGCCCCCGT 
GACGATGACG AACGCAGTCT CAAAACCATC CAGGAGAACG ATACCGATAA GGATGACACC 
GTGACGGAAG ATTGCACATC GGAAGCGTTT GGGGATTCGG AAGACGACGA TGAAAATCCT 
CGAATTCAGA GAGGCGAGGA CGATGACGGC ATGGATGACG ACGTGGTTGA CGATGACGAC 
GAGGGTGATC TGAATCAAGA CCTCGATCCA CTGATAAAGC CTGCGCCAGT TGAAAAAACG 
ATCGAGGATT TGGAGTTCGA GAAAGAATTC GAACGGATGG CAACGGATAG CTTCTTGGAG 
CGCTTGAAGG AACCGGTGAA ACCCAATGTC AAGGACATTC CCGTTCCGAT TACAATGCGA 
AGCAGCGGAG CAAAGAAAAC ATATGAGCAA CTGCAGGAAA ATAAGGAAAA AACTGACACT 
GTTCCGTTCG TACTCATGGT CAGAGGTAAG GGCAAGCAAC AGCAGTACAA GTCATTTGAA 
GCACCATCGG ACAGTCAGCT GGCAATATAC GTCAAAGATC AGGAGCAGAA GATGAAGGAA 
GAAAACGATA GTGTTAAACG ACTAACGCTG AATATCACCG AACGACTGGA AGAGGAAGAT 
TACCAGGAGT CACTGCTCCA GAGTCAACGA TCGCCAGCAA GTACCAAAAT ACGGATACAA 
AAGCCTCCCA AATTCAAGCA TCCCAAGGGT GTCCCAGATG TTGATGCAAT TTTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001660932.2
CDS	134..3553
Translation	MESTMNSDCGSERKSEEVVEKEESPTKDSEAAKKELDDYVAELSGKYEQKSQLRSQNITPERPPESFFTKCDSSLKKNTAFVKKLKQFTASQLDGLLKDMAGLNLTKYISEVSAAIVEAKLKMSDVVPALMLCSKLHQTYAEFSPSFLENWQKILHVKAGEKIGNPSKMRVDLRFYAELISVGIFSNKVGLPLLGACLTALISQDKDEHSNLSIILSFCRHCGDEYAGLVPKKIMELAKQFEVVIPSSNLLTSDKQQNLRNLLKDYYQTVCEHLTNEHKQLQYAEKSRRRMLESKGEVPNEKKEQIEVLQNSYEKLLSSAQTMADLLNETLPELTVEAEACPSEGNVIDSIGDEQFANLDPWGDEDTRNFYVNLPDLRQFLPNYCGKKEEIIPDEPAMTEEVLDMEVEQEQDTEEVGDPPSEISETAEPEEEPTTAPSTPHHHANREHFEAFIANLQNCVNTELIDSAAIEFLLNLNTKNNRKRLVKVLFGVQRTRLDLLPMYARLVAIINLVSADVAIDLCQMLKVDFKYQIKKKDQINIETKIKVVRYIGELVKFGLYNKIEALFCLKCLLHNFQHHHIEMTCAFLEVCGVYLYNSKDSRLRTNVYLEQMMRLKTNTTMDSRHAQQVETAYYLVKQPEGMRIQRKVRPLFHTYIRHLIFEELNKSNVDKMIKLLRRLDWEDRDIHDYAVKCLSKAYSIRYHLIRSLADLVSGLSSYQEKAVVKVIDAVFEDIRAGLEIHDTKLAQRRIAMAKYLGELYNYRLVESKNVLNTLYSIISLGVSASHDTPSEVDPPGSLFRLKLVCVLLDTCGQYFTSASSRKGLDYFLIFFQNYYWFKKSDPIFTSETNKDLFPILVDHMYKECFKNLRPKLKLLTSYEASKEAIENIAKKVYPQLFSPRDDDERSLKTIQENDTDKDDTVTEDCTSEAFGDSEDDDENPRIQRGEDDDGMDDDVVDDDDEGDLNQDLDPLIKPAPVEKTIEDLEFEKEFERMATDSFLERLKEPVKPNVKDIPVPITMRSSGAKKTYEQLQENKEKTDTVPFVLMVRGKGKQQQYKSFEAPSDSQLAIYVKDQEQKMKEENDSVKRLTLNITERLEEEDYQESLLQSQRSPASTKIRIQKPPKFKHPKGVPDVDAIFH

Target ORF information:

RefSeq Version	XM_001660882.2
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti regulator of nonsense transcripts 2 (LOC5573561), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001660882.2

ORF Insert Sequence:

ATGGAATCCA CCATGAATTC CGATTGCGGA TCGGAACGGA AGAGCGAGGA AGTGGTGGAA 
AAGGAAGAAT CGCCTACCAA AGACTCGGAA GCTGCCAAGA AGGAGTTGGA CGATTATGTG 
GCGGAACTCA GCGGAAAATA CGAGCAGAAA TCTCAGCTGC GATCGCAGAA TATTACTCCG 
GAACGTCCAC CGGAATCGTT CTTCACCAAA TGTGATTCAA GCTTGAAGAA AAATACGGCG 
TTTGTGAAGA AGTTGAAGCA ATTTACTGCC TCGCAGTTGG ATGGTTTGCT GAAGGATATG 
GCCGGGTTGA ATTTGACAAA GTACATTTCG GAAGTCAGTG CCGCGATTGT GGAAGCCAAA 
CTGAAGATGA GTGATGTGGT TCCGGCTCTG ATGTTATGCA GCAAACTTCA CCAGACCTAT 
GCGGAGTTCA GTCCGAGCTT TTTGGAGAAC TGGCAGAAGA TTTTGCACGT CAAAGCTGGT 
GAGAAAATTG GGAATCCGAG CAAGATGAGG GTGGACTTGA GATTCTACGC TGAACTGATC 
AGTGTTGGAA TCTTCAGTAA TAAGGTTGGA TTGCCTTTGC TGGGAGCATG CTTAACAGCT 
TTGATATCCC AGGATAAGGA CGAGCATAGT AATTTGTCCA TAATCCTATC ATTCTGTCGA 
CATTGTGGCG ATGAGTATGC TGGATTAGTG CCCAAGAAGA TTATGGAACT AGCCAAGCAA 
TTCGAAGTGG TTATTCCTTC GTCAAATCTA CTCACCTCGG ATAAACAGCA AAATCTACGG 
AACCTACTGA AAGACTACTA TCAGACAGTC TGTGAGCATT TAACGAACGA GCACAAACAA 
CTACAATATG CGGAGAAATC TAGAAGAAGG ATGCTGGAAT CGAAAGGAGA GGTTCCAAAC 
GAAAAGAAAG AGCAAATCGA AGTGCTTCAG AACAGTTACG AAAAACTGTT ATCGTCTGCC 
CAAACCATGG CCGATCTATT AAATGAAACA CTACCGGAAC TTACCGTTGA AGCAGAAGCG 
TGTCCATCGG AAGGAAACGT TATTGATAGC ATTGGAGATG AGCAATTTGC CAATCTGGAT 
CCTTGGGGCG ATGAAGATAC TCGCAATTTC TACGTCAATC TGCCGGATCT GCGACAGTTC 
CTGCCAAACT ACTGCGGAAA GAAAGAGGAA ATTATCCCGG ATGAACCTGC CATGACGGAG 
GAAGTGCTCG ATATGGAAGT TGAACAAGAG CAAGACACCG AGGAAGTGGG CGATCCTCCC 
TCGGAGATAT CCGAAACTGC GGAACCGGAA GAAGAACCCA CGACAGCTCC CAGCACCCCA 
CATCACCATG CTAACCGGGA ACACTTCGAA GCATTTATTG CGAATCTGCA AAATTGTGTT 
AATACCGAAT TGATCGATTC GGCCGCGATC GAATTCCTGC TAAACCTAAA CACAAAAAAT 
AACCGGAAAA GACTTGTGAA AGTTCTTTTT GGAGTGCAGA GAACGCGGTT GGATCTCCTC 
CCCATGTACG CAAGATTAGT TGCTATCATC AATCTGGTTT CGGCGGATGT GGCGATAGAT 
CTGTGCCAGA TGTTGAAGGT GGACTTCAAA TATCAGATCA AGAAGAAGGA TCAAATCAAT 
ATCGAGACCA AGATCAAAGT GGTGCGGTAC ATCGGAGAGC TCGTGAAGTT CGGACTGTAT 
AACAAAATTG AAGCGCTGTT CTGTTTGAAG TGCCTGCTAC ACAATTTCCA ACATCACCAC 
ATTGAAATGA CGTGCGCTTT TTTGGAAGTT TGCGGAGTTT ATCTGTACAA TTCGAAAGAC 
AGTCGGCTTC GAACCAATGT ATATCTCGAG CAAATGATGC GTCTCAAAAC CAACACTACG 
ATGGACAGTC GACACGCCCA GCAGGTGGAA ACAGCTTACT ATCTCGTCAA ACAACCGGAG 
GGAATGCGCA TCCAGCGCAA AGTTCGTCCA CTTTTCCACA CCTACATCCG ACATCTGATT 
TTTGAAGAGC TCAACAAGTC CAACGTAGAC AAAATGATTA AACTGTTGCG TCGCTTGGAC 
TGGGAAGATC GTGACATTCA CGACTATGCA GTCAAGTGTC TTTCGAAGGC TTACAGCATT 
CGGTATCACT TAATACGAAG TTTGGCCGAT TTGGTATCAG GATTGAGCTC ATATCAGGAA 
AAGGCCGTTG TAAAAGTGAT CGATGCAGTA TTTGAAGATA TCCGTGCGGG ACTAGAAATC 
CACGACACCA AACTCGCCCA GCGAAGGATT GCAATGGCTA AGTATCTTGG CGAGCTGTAT 
AACTATCGAT TGGTGGAATC GAAAAATGTT CTGAACACAC TATACTCGAT AATATCACTC 
GGGGTCAGTG CCAGCCACGA TACACCATCG GAAGTGGATC CTCCAGGAAG TTTATTCAGG 
CTCAAGCTGG TTTGCGTTCT CCTGGATACA TGTGGACAGT ACTTTACCAG CGCGTCGAGC 
CGCAAGGGCT TGGACTACTT CCTGATATTC TTCCAAAATT ACTACTGGTT CAAAAAATCC 
GATCCTATTT TTACCAGCGA GACGAACAAG GACCTCTTCC CTATACTGGT TGATCATATG 
TATAAGGAAT GTTTTAAAAA TCTACGACCA AAACTAAAGC TATTAACCAG CTACGAGGCA 
TCCAAGGAAG CAATAGAGAA TATAGCGAAG AAAGTCTATC CCCAGTTGTT CAGCCCCCGT 
GACGATGACG AACGCAGTCT CAAAACCATC CAGGAGAACG ATACCGATAA GGATGACACC 
GTGACGGAAG ATTGCACATC GGAAGCGTTT GGGGATTCGG AAGACGACGA TGAAAATCCT 
CGAATTCAGA GAGGCGAGGA CGATGACGGC ATGGATGACG ACGTGGTTGA CGATGACGAC 
GAGGGTGATC TGAATCAAGA CCTCGATCCA CTGATAAAGC CTGCGCCAGT TGAAAAAACG 
ATCGAGGATT TGGAGTTCGA GAAAGAATTC GAACGGATGG CAACGGATAG CTTCTTGGAG 
CGCTTGAAGG AACCGGTGAA ACCCAATGTC AAGGACATTC CCGTTCCGAT TACAATGCGA 
AGCAGCGGAG CAAAGAAAAC ATATGAGCAA CTGCAGGAAA ATAAGGAAAA AACTGACACT 
GTTCCGTTCG TACTCATGGT CAGAGGTAAG GGCAAGCAAC AGCAGTACAA GTCATTTGAA 
GCACCATCGG ACAGTCAGCT GGCAATATAC GTCAAAGATC AGGAGCAGAA GATGAAGGAA 
GAAAACGATA GTGTTAAACG ACTAACGCTG AATATCACCG AACGACTGGA AGAGGAAGAT 
TACCAGGAGT CACTGCTCCA GAGTCAACGA TCGCCAGCAA GTACCAAAAT ACGGATACAA 
AAGCCTCCCA AATTCAAGCA TCCCAAGGGT GTCCCAGATG TTGATGCAAT TTTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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AAEL_AAEL010598 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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