LOC5571253 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC5571253
Entrez Gene ID	5571253
Full Name	tolloid-like protein 1
Synonyms	AAEL001566
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001653501.1	XP_001653551.1	AAEL001566-PA
XM_001653501.2	XP_001653551.1	tolloid-like protein 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC5571253 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC5571253 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe06709	XM_001653501.2 Latest version!	Aedes aegypti tolloid-like protein 1 (LOC5571253), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OAe06709	XM_001653501.1	Aedes aegypti AAEL001566-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC5571253 ( XM_001653501.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001653551.1 Aedes aegypti AAEL001566-RA partial mRNA.

Price & Availability↑

CloneID	OAe06709
Clone ID Related Accession (Same CDS sequence)	XM_001653501.1 , XM_001653501.2
Accession Version	XM_001653501.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL001566-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001811085). COMPLETENESS: incomplete on both ends.

ATGTTGTCCT TGATGGTTCG TTGTTCCTGG TGGAATCTGA GATGGAGCTT GGTGTTGATG 
GTGATGGTGG CGATGATCAA GGTGGAATGC GTGCAGCGAT CTTACACAGT TGATGAGCTA 
CTTCAAAGTA CCTTCCCGAT TGGGATATCT AACGATATTG ATATGGACCC GTGCAAGTCA 
AGGGGGTTCA TGGGTGATAT TGCACTGCCG AATGTGAACT ATGAGAGTGA ATTCCATAAA 
CAAAGACTGA GACAAAAAGA GGAGGAAGAG ATGCTAAAAA GAGAGATCGA GGAAGGTCTA 
CAGGTAGAAG CAGAAGGGCT AACTACGGGG GCAGCACCTG TTTCAACGGG TAGACATCCT 
AGCGATAAGA GAAGTGGTAG TGAGCTCGTG AATCGATCAC CGAAGATGAA TGTAAACAAA 
AGTGTAAAAT ACAGGAAACG ACACAATAAA CGGCCAATAA AGGACAGCGA TCGTAGTGAC 
GGTGGAGCAA GATCATCGGA TTCTCGGAAA GTGGGGCGGC GAAGTATGGG TCATAAACCG 
ATTACGCCAC GTAAAATGCG ACGATCGAAG GGTAAACAAG CTAATCGAGT GCTACACAGA 
TCAACTCGAG CAGCGACGGC AAAAAAGGAA CGAATCTGGG ACTTTGGTGT GATCCCATAC 
GAGATTGATG GTAACTTCAG TGGTATGCAT AAGGCTCTCT TCAAACAGGC CATGCGCCAC 
TGGGAGAACC ATACGTGCAT CAAATTTGTT GAGCGCGATC CAGAAGTTCA TCCTAACTTC 
ATTGTGTTCA CGGAACGACC ATGTGGCTGT TGCTCGTTCG TTGGCAAACG AGGAAACGGA 
CCTCAGGCGA TCTCGATCGG TAAGAACTGC GACAAGTTTG GCATTGTGGT ACATGAACTC 
GGTCACGTGG TAGGGTTTTG GCACGAACAC ACCCGCCCAG ATCGTGAAGA TCACGTTGTC 
ATAGAAAAGC ATAACATCAT GGTCGGTCAG GAATACAACT TCAACAAACT CACCGAAGAT 
GAAGTCAACT CATTGGGCCA GCCTTACGAT TATTTCTCGA TCATGCACTA CGCTAGGAAT 
ACGTTCTCCA AGGGCACTTA CTTGGACACT ATCCTGCCTG TTGATATGCC AGGGCAAAAA 
CGTCCAGAAA TCGGTCAACG CCTGAGATTA AGCGAAGGCG ACATCATCCA AGCAAACCTA 
TTGTACAAAT GCGCTAAGTG CGGCCGCACA TTCCAAGATC ACTCGGGGTC CTTCACGTCC 
CCTAGCTACT ACTCGAAAAC GCCTCCCATC GAACCGGAGC GCTGCGAGTG GCGAATAACA 
GCAACTCACG GAGAGCAGAT CGTGCTCAAC ATAACCGATC TGGATATTTT CAAGTCCGAT 
AACTGCCGTA CTGATTACCT GGAGATTCGA GACGGCTACT GGCACAAGTC TCCTTTGTTG 
GGGAAGTTTT GCGGGTCAGG TCGAAACAGC AATCTCATAG TATCCACAGG GAGCCGAATG 
CTGCTCAATT TTGTAACGAG TTTCCGTCAG GCTGGTAAGC GTGGATTTGC CGCTAACTAT 
GAAGCTGTTT GTGGCGGCGA AATTCATCTG GACAGCAGTG GTGGTCGTCT GGAGTCACCG 
AACTACCCCG CAGATTATCT CCCTAACAAG GAATGCGTTT GGAAGATTAC CGTACCGGAG 
GACTACCAAG TGGCGATCAA GTTTCAATCT TTCGAAGTAG AAAATCACGA TAGCTGCGCG 
TACGACTACG TTGAAATTCG TGACGGGGAT GAGGAAGATT CTAGAGAGAT TGGAATCTTT 
TGTGGTTATA AGCTGCCACC GGATATGAGA TCATCTGGGA ACACCATGCT AGTGAAGTTC 
TTTTCGGACG GTTCGGTGCA GAAAGCTGGA TTCTTGGCAA CGTTGGTTCG GGAGGTGGAC 
GAATGCGAAC GAATGGAGCA TAGCTGCGAA CATGAATGTA TTAATACGCT CGGTGGATAC 
GAGTGCGGTT GTCAAATCGG ATATGAACTG CATAGCGATC GCAAGTCCTG TGAAAAGGCA 
TGTGGAGGAA TTCTTTCGCA AAGAAGTGGT ACCGTCGTTT CACCATCTTT CCCCAGAGAA 
TACCCGGCGA TGAAGGATTG CGCTTGGGAG ATTGTTGCAT CTCGGACGCA TAAAATTACA 
CTTAATTTTA CTCATTTTGA CTTGGAAGGG AACGTATTCT ATCAACCATC GGAGTGCGAT 
TACGATGCTG TTACAATATA TTCCAGGATC GGCGAAGAAG CATTGAAAAA GCATGGGACG 
TATTGTGGCT CGAAACTTCC GCCGGTGATC ACTTCAGAGA GTCACATTCT TCGGTTGGAG 
TTCCGTTCGG ATAAGACAGT GCAGAAGACT GGGTTCGCAG CAGTTTTTTT CACGGACATC 
GATGAGTGCG CCGTCAACAA CGGAGGTTGT CAGCATGAAT GTAAGAATAC GGTGGGATCG 
TATGTTTGTT TATGTCGAAA TGGCTACATA TTGCACGAAA ACGGCCATGA CTGTAAGGAA 
GGCGGCTGTA AGTTTGAGAT AACAGCCCCA ACTGGACAAA TCTTCAGTCC AAACTATCCG 
GATGACTATC CAGCGAAAAA GGATTGCATC TGGCATTTCA CGACCGCTCC TGGTCACCGG 
ATCAAACTTG TGTTCAGCAT CTTCGACGTC GAGCTTCATC AGGAGTGCGC GTATGACAAC 
GTTGCAATTT ATGACGGTGA TTCCCAGGAT AGCTACATAC TTGGTCGATT CTGTGGAGCA 
AAGCTTCCTC ATCCGATATC GTCTACATCG AACGAGATGT ACATGGTGTT CAACACGGAC 
GCTAGTGTCC AACGTAAGGG TTTCTTTGCT ACTCACACCA CGGCTTGCGG AGGGTATCTG 
CAAGCCACCT ACGAAATCAA CCACTTTTAC TCTCACGTAA GTTACGGCGA TGGAGTTTAT 
GATAATGGCG TCGACTGCGA GTGGACTATT GAAGCTGATC CTGGCCGCAA AGTTCAATTG 
ACTTTCCTTA CATTTGATCT GGAAGAGGAG AAAACGTGCT CGTACGATTA CGTTGAACTA 
GCGAGTGGAT TCGACGATAC CGCCAGCGGA CAACTGCAGG GCAAGTACTG CGGTAATCGT 
CTGCCAGCGG AGGTAATTTC GATGAGCGAA GTCATTGTCG TAAGGTTCCG CTCCGACGAT 
ACGGTTGGCT TCAAGGGCTT CTCTGCGGCG TACGTTGCCA TCAATCCATA TGACGATGAT 
GAACTTTCTA CGGCCGAAGA TACGTACGAA AGCAATGAGC AGGCCGCACC GTTTCCGGGT 
TCGTTAAGAA GTATGTTCAT CAGTCAGGAT GAATACGATG CAGATGATGA TGATGCGGCC 
GGGGAAGACG ATGCGGACTA TGCTGCGTAT CACCATCGGA GGCATCATCA TAAAAGTAAA 
GTGCAATTGG GGGTTCACAA CGGGTTGGGA GCGGCTCAAA AGACGACAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001653551.1
CDS	1..3474
Translation	MLSLMVRCSWWNLRWSLVLMVMVAMIKVECVQRSYTVDELLQSTFPIGISNDIDMDPCKSRGFMGDIALPNVNYESEFHKQRLRQKEEEEMLKREIEEGLQVEAEGLTTGAAPVSTGRHPSDKRSGSELVNRSPKMNVNKSVKYRKRHNKRPIKDSDRSDGGARSSDSRKVGRRSMGHKPITPRKMRRSKGKQANRVLHRSTRAATAKKERIWDFGVIPYEIDGNFSGMHKALFKQAMRHWENHTCIKFVERDPEVHPNFIVFTERPCGCCSFVGKRGNGPQAISIGKNCDKFGIVVHELGHVVGFWHEHTRPDREDHVVIEKHNIMVGQEYNFNKLTEDEVNSLGQPYDYFSIMHYARNTFSKGTYLDTILPVDMPGQKRPEIGQRLRLSEGDIIQANLLYKCAKCGRTFQDHSGSFTSPSYYSKTPPIEPERCEWRITATHGEQIVLNITDLDIFKSDNCRTDYLEIRDGYWHKSPLLGKFCGSGRNSNLIVSTGSRMLLNFVTSFRQAGKRGFAANYEAVCGGEIHLDSSGGRLESPNYPADYLPNKECVWKITVPEDYQVAIKFQSFEVENHDSCAYDYVEIRDGDEEDSREIGIFCGYKLPPDMRSSGNTMLVKFFSDGSVQKAGFLATLVREVDECERMEHSCEHECINTLGGYECGCQIGYELHSDRKSCEKACGGILSQRSGTVVSPSFPREYPAMKDCAWEIVASRTHKITLNFTHFDLEGNVFYQPSECDYDAVTIYSRIGEEALKKHGTYCGSKLPPVITSESHILRLEFRSDKTVQKTGFAAVFFTDIDECAVNNGGCQHECKNTVGSYVCLCRNGYILHENGHDCKEGGCKFEITAPTGQIFSPNYPDDYPAKKDCIWHFTTAPGHRIKLVFSIFDVELHQECAYDNVAIYDGDSQDSYILGRFCGAKLPHPISSTSNEMYMVFNTDASVQRKGFFATHTTACGGYLQATYEINHFYSHVSYGDGVYDNGVDCEWTIEADPGRKVQLTFLTFDLEEEKTCSYDYVELASGFDDTASGQLQGKYCGNRLPAEVISMSEVIVVRFRSDDTVGFKGFSAAYVAINPYDDDELSTAEDTYESNEQAAPFPGSLRSMFISQDEYDADDDDAAGEDDADYAAYHHRRHHHKSKVQLGVHNGLGAAQKTTD

Target ORF information:

RefSeq Version	XM_001653501.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL001566-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001653501.1

ORF Insert Sequence:

ATGTTGTCCT TGATGGTTCG TTGTTCCTGG TGGAATCTGA GATGGAGCTT GGTGTTGATG 
GTGATGGTGG CGATGATCAA GGTGGAATGC GTGCAGCGAT CTTACACAGT TGATGAGCTA 
CTTCAAAGTA CCTTCCCGAT TGGGATATCT AACGATATTG ATATGGACCC GTGCAAGTCA 
AGGGGGTTCA TGGGTGATAT TGCACTGCCG AATGTGAACT ATGAGAGTGA ATTCCATAAA 
CAAAGACTGA GACAAAAAGA GGAGGAAGAG ATGCTAAAAA GAGAGATCGA GGAAGGTCTA 
CAGGTAGAAG CAGAAGGGCT AACTACGGGG GCAGCACCTG TTTCAACGGG TAGACATCCT 
AGCGATAAGA GAAGTGGTAG TGAGCTCGTG AATCGATCAC CGAAGATGAA TGTAAACAAA 
AGTGTAAAAT ACAGGAAACG ACACAATAAA CGGCCAATAA AGGACAGCGA TCGTAGTGAC 
GGTGGAGCAA GATCATCGGA TTCTCGGAAA GTGGGGCGGC GAAGTATGGG TCATAAACCG 
ATTACGCCAC GTAAAATGCG ACGATCGAAG GGTAAACAAG CTAATCGAGT GCTACACAGA 
TCAACTCGAG CAGCGACGGC AAAAAAGGAA CGAATCTGGG ACTTTGGTGT GATCCCATAC 
GAGATTGATG GTAACTTCAG TGGTATGCAT AAGGCTCTCT TCAAACAGGC CATGCGCCAC 
TGGGAGAACC ATACGTGCAT CAAATTTGTT GAGCGCGATC CAGAAGTTCA TCCTAACTTC 
ATTGTGTTCA CGGAACGACC ATGTGGCTGT TGCTCGTTCG TTGGCAAACG AGGAAACGGA 
CCTCAGGCGA TCTCGATCGG TAAGAACTGC GACAAGTTTG GCATTGTGGT ACATGAACTC 
GGTCACGTGG TAGGGTTTTG GCACGAACAC ACCCGCCCAG ATCGTGAAGA TCACGTTGTC 
ATAGAAAAGC ATAACATCAT GGTCGGTCAG GAATACAACT TCAACAAACT CACCGAAGAT 
GAAGTCAACT CATTGGGCCA GCCTTACGAT TATTTCTCGA TCATGCACTA CGCTAGGAAT 
ACGTTCTCCA AGGGCACTTA CTTGGACACT ATCCTGCCTG TTGATATGCC AGGGCAAAAA 
CGTCCAGAAA TCGGTCAACG CCTGAGATTA AGCGAAGGCG ACATCATCCA AGCAAACCTA 
TTGTACAAAT GCGCTAAGTG CGGCCGCACA TTCCAAGATC ACTCGGGGTC CTTCACGTCC 
CCTAGCTACT ACTCGAAAAC GCCTCCCATC GAACCGGAGC GCTGCGAGTG GCGAATAACA 
GCAACTCACG GAGAGCAGAT CGTGCTCAAC ATAACCGATC TGGATATTTT CAAGTCCGAT 
AACTGCCGTA CTGATTACCT GGAGATTCGA GACGGCTACT GGCACAAGTC TCCTTTGTTG 
GGGAAGTTTT GCGGGTCAGG TCGAAACAGC AATCTCATAG TATCCACAGG GAGCCGAATG 
CTGCTCAATT TTGTAACGAG TTTCCGTCAG GCTGGTAAGC GTGGATTTGC CGCTAACTAT 
GAAGCTGTTT GTGGCGGCGA AATTCATCTG GACAGCAGTG GTGGTCGTCT GGAGTCACCG 
AACTACCCCG CAGATTATCT CCCTAACAAG GAATGCGTTT GGAAGATTAC CGTACCGGAG 
GACTACCAAG TGGCGATCAA GTTTCAATCT TTCGAAGTAG AAAATCACGA TAGCTGCGCG 
TACGACTACG TTGAAATTCG TGACGGGGAT GAGGAAGATT CTAGAGAGAT TGGAATCTTT 
TGTGGTTATA AGCTGCCACC GGATATGAGA TCATCTGGGA ACACCATGCT AGTGAAGTTC 
TTTTCGGACG GTTCGGTGCA GAAAGCTGGA TTCTTGGCAA CGTTGGTTCG GGAGGTGGAC 
GAATGCGAAC GAATGGAGCA TAGCTGCGAA CATGAATGTA TTAATACGCT CGGTGGATAC 
GAGTGCGGTT GTCAAATCGG ATATGAACTG CATAGCGATC GCAAGTCCTG TGAAAAGGCA 
TGTGGAGGAA TTCTTTCGCA AAGAAGTGGT ACCGTCGTTT CACCATCTTT CCCCAGAGAA 
TACCCGGCGA TGAAGGATTG CGCTTGGGAG ATTGTTGCAT CTCGGACGCA TAAAATTACA 
CTTAATTTTA CTCATTTTGA CTTGGAAGGG AACGTATTCT ATCAACCATC GGAGTGCGAT 
TACGATGCTG TTACAATATA TTCCAGGATC GGCGAAGAAG CATTGAAAAA GCATGGGACG 
TATTGTGGCT CGAAACTTCC GCCGGTGATC ACTTCAGAGA GTCACATTCT TCGGTTGGAG 
TTCCGTTCGG ATAAGACAGT GCAGAAGACT GGGTTCGCAG CAGTTTTTTT CACGGACATC 
GATGAGTGCG CCGTCAACAA CGGAGGTTGT CAGCATGAAT GTAAGAATAC GGTGGGATCG 
TATGTTTGTT TATGTCGAAA TGGCTACATA TTGCACGAAA ACGGCCATGA CTGTAAGGAA 
GGCGGCTGTA AGTTTGAGAT AACAGCCCCA ACTGGACAAA TCTTCAGTCC AAACTATCCG 
GATGACTATC CAGCGAAAAA GGATTGCATC TGGCATTTCA CGACCGCTCC TGGTCACCGG 
ATCAAACTTG TGTTCAGCAT CTTCGACGTC GAGCTTCATC AGGAGTGCGC GTATGACAAC 
GTTGCAATTT ATGACGGTGA TTCCCAGGAT AGCTACATAC TTGGTCGATT CTGTGGAGCA 
AAGCTTCCTC ATCCGATATC GTCTACATCG AACGAGATGT ACATGGTGTT CAACACGGAC 
GCTAGTGTCC AACGTAAGGG TTTCTTTGCT ACTCACACCA CGGCTTGCGG AGGGTATCTG 
CAAGCCACCT ACGAAATCAA CCACTTTTAC TCTCACGTAA GTTACGGCGA TGGAGTTTAT 
GATAATGGCG TCGACTGCGA GTGGACTATT GAAGCTGATC CTGGCCGCAA AGTTCAATTG 
ACTTTCCTTA CATTTGATCT GGAAGAGGAG AAAACGTGCT CGTACGATTA CGTTGAACTA 
GCGAGTGGAT TCGACGATAC CGCCAGCGGA CAACTGCAGG GCAAGTACTG CGGTAATCGT 
CTGCCAGCGG AGGTAATTTC GATGAGCGAA GTCATTGTCG TAAGGTTCCG CTCCGACGAT 
ACGGTTGGCT TCAAGGGCTT CTCTGCGGCG TACGTTGCCA TCAATCCATA TGACGATGAT 
GAACTTTCTA CGGCCGAAGA TACGTACGAA AGCAATGAGC AGGCCGCACC GTTTCCGGGT 
TCGTTAAGAA GTATGTTCAT CAGTCAGGAT GAATACGATG CAGATGATGA TGATGCGGCC 
GGGGAAGACG ATGCGGACTA TGCTGCGTAT CACCATCGGA GGCATCATCA TAAAAGTAAA 
GTGCAATTGG GGGTTCACAA CGGGTTGGGA GCGGCTCAAA AGACGACAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5571253 ( XM_001653501.2 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001653551.1 Aedes aegypti tolloid-like protein 1 (LOC5571253), mRNA.

Price & Availability↑

CloneID	OAe06709
Clone ID Related Accession (Same CDS sequence)	XM_001653501.1 , XM_001653501.2
Accession Version	XM_001653501.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	tolloid-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 20, 2017 this sequence version replaced XM_001653501.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCCT TGATGGTTCG TTGTTCCTGG TGGAATCTGA GATGGAGCTT GGTGTTGATG 
GTGATGGTGG CGATGATCAA GGTGGAATGC GTGCAGCGAT CTTACACAGT TGATGAGCTA 
CTTCAAAGTA CCTTCCCGAT TGGGATATCT AACGATATTG ATATGGACCC GTGCAAGTCA 
AGGGGGTTCA TGGGTGATAT TGCACTGCCG AATGTGAACT ATGAGAGTGA ATTCCATAAA 
CAAAGACTGA GACAAAAAGA GGAGGAAGAG ATGCTAAAAA GAGAGATCGA GGAAGGTCTA 
CAGGTAGAAG CAGAAGGGCT AACTACGGGG GCAGCACCTG TTTCAACGGG TAGACATCCT 
AGCGATAAGA GAAGTGGTAG TGAGCTCGTG AATCGATCAC CGAAGATGAA TGTAAACAAA 
AGTGTAAAAT ACAGGAAACG ACACAATAAA CGGCCAATAA AGGACAGCGA TCGTAGTGAC 
GGTGGAGCAA GATCATCGGA TTCTCGGAAA GTGGGGCGGC GAAGTATGGG TCATAAACCG 
ATTACGCCAC GTAAAATGCG ACGATCGAAG GGTAAACAAG CTAATCGAGT GCTACACAGA 
TCAACTCGAG CAGCGACGGC AAAAAAGGAA CGAATCTGGG ACTTTGGTGT GATCCCATAC 
GAGATTGATG GTAACTTCAG TGGTATGCAT AAGGCTCTCT TCAAACAGGC CATGCGCCAC 
TGGGAGAACC ATACGTGCAT CAAATTTGTT GAGCGCGATC CAGAAGTTCA TCCTAACTTC 
ATTGTGTTCA CGGAACGACC ATGTGGCTGT TGCTCGTTCG TTGGCAAACG AGGAAACGGA 
CCTCAGGCGA TCTCGATCGG TAAGAACTGC GACAAGTTTG GCATTGTGGT ACATGAACTC 
GGTCACGTGG TAGGGTTTTG GCACGAACAC ACCCGCCCAG ATCGTGAAGA TCACGTTGTC 
ATAGAAAAGC ATAACATCAT GGTCGGTCAG GAATACAACT TCAACAAACT CACCGAAGAT 
GAAGTCAACT CATTGGGCCA GCCTTACGAT TATTTCTCGA TCATGCACTA CGCTAGGAAT 
ACGTTCTCCA AGGGCACTTA CTTGGACACT ATCCTGCCTG TTGATATGCC AGGGCAAAAA 
CGTCCAGAAA TCGGTCAACG CCTGAGATTA AGCGAAGGCG ACATCATCCA AGCAAACCTA 
TTGTACAAAT GCGCTAAGTG CGGCCGCACA TTCCAAGATC ACTCGGGGTC CTTCACGTCC 
CCTAGCTACT ACTCGAAAAC GCCTCCCATC GAACCGGAGC GCTGCGAGTG GCGAATAACA 
GCAACTCACG GAGAGCAGAT CGTGCTCAAC ATAACCGATC TGGATATTTT CAAGTCCGAT 
AACTGCCGTA CTGATTACCT GGAGATTCGA GACGGCTACT GGCACAAGTC TCCTTTGTTG 
GGGAAGTTTT GCGGGTCAGG TCGAAACAGC AATCTCATAG TATCCACAGG GAGCCGAATG 
CTGCTCAATT TTGTAACGAG TTTCCGTCAG GCTGGTAAGC GTGGATTTGC CGCTAACTAT 
GAAGCTGTTT GTGGCGGCGA AATTCATCTG GACAGCAGTG GTGGTCGTCT GGAGTCACCG 
AACTACCCCG CAGATTATCT CCCTAACAAG GAATGCGTTT GGAAGATTAC CGTACCGGAG 
GACTACCAAG TGGCGATCAA GTTTCAATCT TTCGAAGTAG AAAATCACGA TAGCTGCGCG 
TACGACTACG TTGAAATTCG TGACGGGGAT GAGGAAGATT CTAGAGAGAT TGGAATCTTT 
TGTGGTTATA AGCTGCCACC GGATATGAGA TCATCTGGGA ACACCATGCT AGTGAAGTTC 
TTTTCGGACG GTTCGGTGCA GAAAGCTGGA TTCTTGGCAA CGTTGGTTCG GGAGGTGGAC 
GAATGCGAAC GAATGGAGCA TAGCTGCGAA CATGAATGTA TTAATACGCT CGGTGGATAC 
GAGTGCGGTT GTCAAATCGG ATATGAACTG CATAGCGATC GCAAGTCCTG TGAAAAGGCA 
TGTGGAGGAA TTCTTTCGCA AAGAAGTGGT ACCGTCGTTT CACCATCTTT CCCCAGAGAA 
TACCCGGCGA TGAAGGATTG CGCTTGGGAG ATTGTTGCAT CTCGGACGCA TAAAATTACA 
CTTAATTTTA CTCATTTTGA CTTGGAAGGG AACGTATTCT ATCAACCATC GGAGTGCGAT 
TACGATGCTG TTACAATATA TTCCAGGATC GGCGAAGAAG CATTGAAAAA GCATGGGACG 
TATTGTGGCT CGAAACTTCC GCCGGTGATC ACTTCAGAGA GTCACATTCT TCGGTTGGAG 
TTCCGTTCGG ATAAGACAGT GCAGAAGACT GGGTTCGCAG CAGTTTTTTT CACGGACATC 
GATGAGTGCG CCGTCAACAA CGGAGGTTGT CAGCATGAAT GTAAGAATAC GGTGGGATCG 
TATGTTTGTT TATGTCGAAA TGGCTACATA TTGCACGAAA ACGGCCATGA CTGTAAGGAA 
GGCGGCTGTA AGTTTGAGAT AACAGCCCCA ACTGGACAAA TCTTCAGTCC AAACTATCCG 
GATGACTATC CAGCGAAAAA GGATTGCATC TGGCATTTCA CGACCGCTCC TGGTCACCGG 
ATCAAACTTG TGTTCAGCAT CTTCGACGTC GAGCTTCATC AGGAGTGCGC GTATGACAAC 
GTTGCAATTT ATGACGGTGA TTCCCAGGAT AGCTACATAC TTGGTCGATT CTGTGGAGCA 
AAGCTTCCTC ATCCGATATC GTCTACATCG AACGAGATGT ACATGGTGTT CAACACGGAC 
GCTAGTGTCC AACGTAAGGG TTTCTTTGCT ACTCACACCA CGGCTTGCGG AGGGTATCTG 
CAAGCCACCT ACGAAATCAA CCACTTTTAC TCTCACGTAA GTTACGGCGA TGGAGTTTAT 
GATAATGGCG TCGACTGCGA GTGGACTATT GAAGCTGATC CTGGCCGCAA AGTTCAATTG 
ACTTTCCTTA CATTTGATCT GGAAGAGGAG AAAACGTGCT CGTACGATTA CGTTGAACTA 
GCGAGTGGAT TCGACGATAC CGCCAGCGGA CAACTGCAGG GCAAGTACTG CGGTAATCGT 
CTGCCAGCGG AGGTAATTTC GATGAGCGAA GTCATTGTCG TAAGGTTCCG CTCCGACGAT 
ACGGTTGGCT TCAAGGGCTT CTCTGCGGCG TACGTTGCCA TCAATCCATA TGACGATGAT 
GAACTTTCTA CGGCCGAAGA TACGTACGAA AGCAATGAGC AGGCCGCACC GTTTCCGGGT 
TCGTTAAGAA GTATGTTCAT CAGTCAGGAT GAATACGATG CAGATGATGA TGATGCGGCC 
GGGGAAGACG ATGCGGACTA TGCTGCGTAT CACCATCGGA GGCATCATCA TAAAAGTAAA 
GTGCAATTGG GGGTTCACAA CGGGTTGGGA GCGGCTCAAA AGACGACAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001653551.1
CDS	354..3827
Translation	MLSLMVRCSWWNLRWSLVLMVMVAMIKVECVQRSYTVDELLQSTFPIGISNDIDMDPCKSRGFMGDIALPNVNYESEFHKQRLRQKEEEEMLKREIEEGLQVEAEGLTTGAAPVSTGRHPSDKRSGSELVNRSPKMNVNKSVKYRKRHNKRPIKDSDRSDGGARSSDSRKVGRRSMGHKPITPRKMRRSKGKQANRVLHRSTRAATAKKERIWDFGVIPYEIDGNFSGMHKALFKQAMRHWENHTCIKFVERDPEVHPNFIVFTERPCGCCSFVGKRGNGPQAISIGKNCDKFGIVVHELGHVVGFWHEHTRPDREDHVVIEKHNIMVGQEYNFNKLTEDEVNSLGQPYDYFSIMHYARNTFSKGTYLDTILPVDMPGQKRPEIGQRLRLSEGDIIQANLLYKCAKCGRTFQDHSGSFTSPSYYSKTPPIEPERCEWRITATHGEQIVLNITDLDIFKSDNCRTDYLEIRDGYWHKSPLLGKFCGSGRNSNLIVSTGSRMLLNFVTSFRQAGKRGFAANYEAVCGGEIHLDSSGGRLESPNYPADYLPNKECVWKITVPEDYQVAIKFQSFEVENHDSCAYDYVEIRDGDEEDSREIGIFCGYKLPPDMRSSGNTMLVKFFSDGSVQKAGFLATLVREVDECERMEHSCEHECINTLGGYECGCQIGYELHSDRKSCEKACGGILSQRSGTVVSPSFPREYPAMKDCAWEIVASRTHKITLNFTHFDLEGNVFYQPSECDYDAVTIYSRIGEEALKKHGTYCGSKLPPVITSESHILRLEFRSDKTVQKTGFAAVFFTDIDECAVNNGGCQHECKNTVGSYVCLCRNGYILHENGHDCKEGGCKFEITAPTGQIFSPNYPDDYPAKKDCIWHFTTAPGHRIKLVFSIFDVELHQECAYDNVAIYDGDSQDSYILGRFCGAKLPHPISSTSNEMYMVFNTDASVQRKGFFATHTTACGGYLQATYEINHFYSHVSYGDGVYDNGVDCEWTIEADPGRKVQLTFLTFDLEEEKTCSYDYVELASGFDDTASGQLQGKYCGNRLPAEVISMSEVIVVRFRSDDTVGFKGFSAAYVAINPYDDDELSTAEDTYESNEQAAPFPGSLRSMFISQDEYDADDDDAAGEDDADYAAYHHRRHHHKSKVQLGVHNGLGAAQKTTD

Target ORF information:

RefSeq Version	XM_001653501.2
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti tolloid-like protein 1 (LOC5571253), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001653501.2

ORF Insert Sequence:

ATGTTGTCCT TGATGGTTCG TTGTTCCTGG TGGAATCTGA GATGGAGCTT GGTGTTGATG 
GTGATGGTGG CGATGATCAA GGTGGAATGC GTGCAGCGAT CTTACACAGT TGATGAGCTA 
CTTCAAAGTA CCTTCCCGAT TGGGATATCT AACGATATTG ATATGGACCC GTGCAAGTCA 
AGGGGGTTCA TGGGTGATAT TGCACTGCCG AATGTGAACT ATGAGAGTGA ATTCCATAAA 
CAAAGACTGA GACAAAAAGA GGAGGAAGAG ATGCTAAAAA GAGAGATCGA GGAAGGTCTA 
CAGGTAGAAG CAGAAGGGCT AACTACGGGG GCAGCACCTG TTTCAACGGG TAGACATCCT 
AGCGATAAGA GAAGTGGTAG TGAGCTCGTG AATCGATCAC CGAAGATGAA TGTAAACAAA 
AGTGTAAAAT ACAGGAAACG ACACAATAAA CGGCCAATAA AGGACAGCGA TCGTAGTGAC 
GGTGGAGCAA GATCATCGGA TTCTCGGAAA GTGGGGCGGC GAAGTATGGG TCATAAACCG 
ATTACGCCAC GTAAAATGCG ACGATCGAAG GGTAAACAAG CTAATCGAGT GCTACACAGA 
TCAACTCGAG CAGCGACGGC AAAAAAGGAA CGAATCTGGG ACTTTGGTGT GATCCCATAC 
GAGATTGATG GTAACTTCAG TGGTATGCAT AAGGCTCTCT TCAAACAGGC CATGCGCCAC 
TGGGAGAACC ATACGTGCAT CAAATTTGTT GAGCGCGATC CAGAAGTTCA TCCTAACTTC 
ATTGTGTTCA CGGAACGACC ATGTGGCTGT TGCTCGTTCG TTGGCAAACG AGGAAACGGA 
CCTCAGGCGA TCTCGATCGG TAAGAACTGC GACAAGTTTG GCATTGTGGT ACATGAACTC 
GGTCACGTGG TAGGGTTTTG GCACGAACAC ACCCGCCCAG ATCGTGAAGA TCACGTTGTC 
ATAGAAAAGC ATAACATCAT GGTCGGTCAG GAATACAACT TCAACAAACT CACCGAAGAT 
GAAGTCAACT CATTGGGCCA GCCTTACGAT TATTTCTCGA TCATGCACTA CGCTAGGAAT 
ACGTTCTCCA AGGGCACTTA CTTGGACACT ATCCTGCCTG TTGATATGCC AGGGCAAAAA 
CGTCCAGAAA TCGGTCAACG CCTGAGATTA AGCGAAGGCG ACATCATCCA AGCAAACCTA 
TTGTACAAAT GCGCTAAGTG CGGCCGCACA TTCCAAGATC ACTCGGGGTC CTTCACGTCC 
CCTAGCTACT ACTCGAAAAC GCCTCCCATC GAACCGGAGC GCTGCGAGTG GCGAATAACA 
GCAACTCACG GAGAGCAGAT CGTGCTCAAC ATAACCGATC TGGATATTTT CAAGTCCGAT 
AACTGCCGTA CTGATTACCT GGAGATTCGA GACGGCTACT GGCACAAGTC TCCTTTGTTG 
GGGAAGTTTT GCGGGTCAGG TCGAAACAGC AATCTCATAG TATCCACAGG GAGCCGAATG 
CTGCTCAATT TTGTAACGAG TTTCCGTCAG GCTGGTAAGC GTGGATTTGC CGCTAACTAT 
GAAGCTGTTT GTGGCGGCGA AATTCATCTG GACAGCAGTG GTGGTCGTCT GGAGTCACCG 
AACTACCCCG CAGATTATCT CCCTAACAAG GAATGCGTTT GGAAGATTAC CGTACCGGAG 
GACTACCAAG TGGCGATCAA GTTTCAATCT TTCGAAGTAG AAAATCACGA TAGCTGCGCG 
TACGACTACG TTGAAATTCG TGACGGGGAT GAGGAAGATT CTAGAGAGAT TGGAATCTTT 
TGTGGTTATA AGCTGCCACC GGATATGAGA TCATCTGGGA ACACCATGCT AGTGAAGTTC 
TTTTCGGACG GTTCGGTGCA GAAAGCTGGA TTCTTGGCAA CGTTGGTTCG GGAGGTGGAC 
GAATGCGAAC GAATGGAGCA TAGCTGCGAA CATGAATGTA TTAATACGCT CGGTGGATAC 
GAGTGCGGTT GTCAAATCGG ATATGAACTG CATAGCGATC GCAAGTCCTG TGAAAAGGCA 
TGTGGAGGAA TTCTTTCGCA AAGAAGTGGT ACCGTCGTTT CACCATCTTT CCCCAGAGAA 
TACCCGGCGA TGAAGGATTG CGCTTGGGAG ATTGTTGCAT CTCGGACGCA TAAAATTACA 
CTTAATTTTA CTCATTTTGA CTTGGAAGGG AACGTATTCT ATCAACCATC GGAGTGCGAT 
TACGATGCTG TTACAATATA TTCCAGGATC GGCGAAGAAG CATTGAAAAA GCATGGGACG 
TATTGTGGCT CGAAACTTCC GCCGGTGATC ACTTCAGAGA GTCACATTCT TCGGTTGGAG 
TTCCGTTCGG ATAAGACAGT GCAGAAGACT GGGTTCGCAG CAGTTTTTTT CACGGACATC 
GATGAGTGCG CCGTCAACAA CGGAGGTTGT CAGCATGAAT GTAAGAATAC GGTGGGATCG 
TATGTTTGTT TATGTCGAAA TGGCTACATA TTGCACGAAA ACGGCCATGA CTGTAAGGAA 
GGCGGCTGTA AGTTTGAGAT AACAGCCCCA ACTGGACAAA TCTTCAGTCC AAACTATCCG 
GATGACTATC CAGCGAAAAA GGATTGCATC TGGCATTTCA CGACCGCTCC TGGTCACCGG 
ATCAAACTTG TGTTCAGCAT CTTCGACGTC GAGCTTCATC AGGAGTGCGC GTATGACAAC 
GTTGCAATTT ATGACGGTGA TTCCCAGGAT AGCTACATAC TTGGTCGATT CTGTGGAGCA 
AAGCTTCCTC ATCCGATATC GTCTACATCG AACGAGATGT ACATGGTGTT CAACACGGAC 
GCTAGTGTCC AACGTAAGGG TTTCTTTGCT ACTCACACCA CGGCTTGCGG AGGGTATCTG 
CAAGCCACCT ACGAAATCAA CCACTTTTAC TCTCACGTAA GTTACGGCGA TGGAGTTTAT 
GATAATGGCG TCGACTGCGA GTGGACTATT GAAGCTGATC CTGGCCGCAA AGTTCAATTG 
ACTTTCCTTA CATTTGATCT GGAAGAGGAG AAAACGTGCT CGTACGATTA CGTTGAACTA 
GCGAGTGGAT TCGACGATAC CGCCAGCGGA CAACTGCAGG GCAAGTACTG CGGTAATCGT 
CTGCCAGCGG AGGTAATTTC GATGAGCGAA GTCATTGTCG TAAGGTTCCG CTCCGACGAT 
ACGGTTGGCT TCAAGGGCTT CTCTGCGGCG TACGTTGCCA TCAATCCATA TGACGATGAT 
GAACTTTCTA CGGCCGAAGA TACGTACGAA AGCAATGAGC AGGCCGCACC GTTTCCGGGT 
TCGTTAAGAA GTATGTTCAT CAGTCAGGAT GAATACGATG CAGATGATGA TGATGCGGCC 
GGGGAAGACG ATGCGGACTA TGCTGCGTAT CACCATCGGA GGCATCATCA TAAAAGTAAA 
GTGCAATTGG GGGTTCACAA CGGGTTGGGA GCGGCTCAAA AGACGACAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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LOC5571253 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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