LOC5570534 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC5570534
Entrez Gene ID	5570534
Full Name	vigilin
Synonyms	AAEL001421
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001659135.2	XP_001659185.1	vigilin
XM_021840756.1	XP_021696448.1	vigilin
XM_001659135.1	XP_001659185.1	AAEL001421-PA
XM_021840757.1	XP_021696449.1	vigilin

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC5570534 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC5570534 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe06046	XM_001659135.1 Latest version!	Aedes aegypti AAEL001421-RA mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAe119094	XM_021840756.1 Latest version!	Aedes aegypti vigilin (LOC5570534), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAe119094	XM_021840757.1 Latest version!	Aedes aegypti vigilin (LOC5570534), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAe119094	XM_001659135.2 Latest version!	Aedes aegypti vigilin (LOC5570534), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC5570534 ( XM_001659135.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001659185.1 Aedes aegypti AAEL001421-RA mRNA.

Price & Availability↑

CloneID	OAe06046
Clone ID Related Accession (Same CDS sequence)	XM_001659135.1
Accession Version	XM_001659135.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL001421-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001811041).

ATGGAGGATT CAACAGTAGT TGACCAGCAG CCCAGCAACT CCGCGTACGA GAACAACGTT 
GCCTCGACGC CGGCTCCGAC CCCGTGCTAC GATGATCTGT TCCCGGCCCT GCCGGAGAGC 
GCCCCACCGC GCTTCAACAA TCCGACCCTG TCGGCGGCCA CGCAGAGTAT GCGCGTCGGC 
AGCTCGGTCG TGACGCAGGT CTTCATCGTG CCGTCCGGCG AGCGCAAGTA CGACTCGGAC 
AAGTTTGGCG AGGGCGAATC GTTGCGCACC TGCCAGAACA TCATGAAGGA AACCAACGCC 
CATATCGAGA TCTCGAGTGG CAAGGACCAA TCGTTGACCT TCCTGGTGAC TGGCAAGCCG 
AACGAGGTGA TCGAGGCCCG CCGTAAGATT TTGGTGCACT TCCAAACTCA GGCGAGCAAG 
TCGATCAGCA TTCCGCGGGA GCACCACCGC TGGATCCTCG GCAAGAAGGG CGACCGGCTG 
CGCGATCTGG AGAAGAACAC CGCCACCAAG ATCGTTGTGC CGCGCATCAA CGACGAGTCG 
GACATCATCA CCATCTCCGG TACCAAGGAA GGCATCGAGA AGGCCGAACA TGAAATCCGT 
ACCACGTCTG ATGAGCAGTC GCGTAAGGCC TTCGAGCGGG TTAACATCCC CAAGATTTAC 
CATCCGTTCA TCATGGGACC CCACAACGAC AACGTCAGCA AGATGATGGA GGAAACCGGA 
GCGAAGATCA ACATTCCGCC GCCGTCCGTC CAAAAGGATG AGATCATCAT CACCGGCGAG 
AAGGAAGGTG TACTCCAGGC AAAGGCACGC ATCGAAGCCA TCAGTAAGGA GATGGAAAAG 
AAGTGTACCA GCGTCGGCGT TGAGGTTCCC CGTGCTCAGC ATAAGTACAT CGTTGGTCCC 
AAGGGTTCCA CCATCCAGGA AATCTTGGCG ATGACCGGTG TATCGGTTGA GATGCCGGCT 
AGTGACTCTC CCAACGATAC CATCACTCTG CGCGGCCCCC AGGACAAGCT CGGAAATGCT 
TTGAGCGTTG TGTACCAGAA GGCGAACTCT GTTCGCACGA CTACGCTCGA GTGTCCCCAG 
TGGATTCATA AGTATATCAT TGGACGCGAG GGTGGCCATA TCAAGGAATT CACCGCTCAG 
TATCCCAACG TACACGTGGA GTTCAACGAA GACAAGATCA AGATCGAAGG CCTACCGGAA 
CAGGTGGATG CTTCTTCGGA AGCGCTGGAG AAAATGGTCA AGGATTACAC TGGACGTCTA 
ACCTTCACCG AAATGGTGGT TGATCCGATC TACTGCAAGC ACATCATCGG TAAGAACGGA 
GCCAACATCA ACCGTATGAA GGAGGAATAC GAGGTCTTGA TCAACATCGA CGAGAAGGAC 
TTGAAACCGA TCCGCATCGA AGGACCGGCC GACGGTGTTG AGAAGGCCAA GCAGGAGCTG 
CTTGAAAAGA TAGCGAAACT GGAAAACGAA AAGGAAGAGT CGATCGTGAT CGATCATCGT 
CTGTTCAAGA CGATCATCGG AGCGAAAGGC GAAACGATTC GGGAAATCCG TGACAAGCAT 
AACCAGGTGC AGATTATCTT CCCCGGAGCT ACCGACAAGT CGGACATTGT CAAGATCCGT 
GGTCCGCGTG ACGATGTGGA TAAGTGTCAC AAGTATCTGA CGAAGTACGT TAAGGAGCTG 
CAGAAGAACT CCTTCGTAAT GGAAGTCCCA ATCTTCAAGC AATTCCACAA GTACATTATC 
GGCAAGGGTG GTGCCAACAT CAAGAAGATT CGTGATGAGA CTCAAACGAA AATCGATCTT 
CCAGCAGAGG GTGACAAAAA CGAGATGATT GTCATCACTG GCAAGAAGGA CAACGTTAAG 
GAGGCTCGCA ATCGCATTCT GAAGATCCAG GAGGAACTTT CCAACATCAT CACCGAGGAT 
ATTCAGATCC CACCCAAGTA CTACAATTCG TTGATCGGAT CTGGTGGCAA GCTGATTTCC 
TCGATTATGG AAGAGTGCGG TGGGGTTTCG ATCAAGTTCC CAAGTCCGGA GTCCAACAGC 
GACAAGGTGG CCATCCGTGG CCCGAAGGAA GATGTCGAAC GAGCTAAGCA ACAGCTGCTC 
GAGCTGACCA ATGAGAAGGA GCTTTCTTCC TTCTCTGCCG AGGTTCGTGC CAAACCGCAG 
CACCATAAGT TCCTCATCGG TAAGAACGGC GCTTCCATCA AGAAGATCCG GGACCAAACC 
GGTGCTCGTA TTGTCTTCCC GGGTTACAAC GACGAGGACA AGGAGGCCAT CATCATCATC 
GGCAAGAAGG AAGGTGTCGA AGAGGCCAAG GCCCAGTTGG AGTCGATTAT CAAGAACATC 
GATAACATTG TTGAAGATGA GATTAGCGTG GAGCCGAGGC ACCATAAGCA TTTCGTGGCT 
CGCCGTGGCG AGGTGCTGCA CCGTATTTCG GAGGAATGCG GTGGCGTGAT GATTTCCTTC 
CCCCGCTCGG GAGTGGACAG CGATCGGGTT ACGCTGAAGG GTGCCAAGGA GTGCATTGAA 
GCGGCCAAGG CTCGCATCCT GGAGATCGTC GACGATTTGG AGCAGATGGT TACGATCGAG 
TGTATCATTC CGGCTCATAA CCATCGCATT GTTATGGGTA AAGGTGGTTC CAAGGTGCAA 
GGAATTACAT CGGAGTTTGA TGTGAACATT AAGTTCCCGG AACGAGACGC GCCGGAAGCG 
TTCGTTGAAG GGCAACAAAA TGGCGATGCA AATGGAGCGG AGCCCATTAA GACGACCGAT 
ATTATCAAGA TTAGTGGTCG CAAAGAAAAG TGCGAAAAGG CAAAGGAAGC TTTGTTGGCT 
TTGGTCCCGG TCACTGAGGA GATTAACGTT GCATTTGATC TGCACAGATC GTTGATTGGC 
CAGAAGGGTC GTGATGTCAA GGAGTTGATG AATACGTACG ATGTTCACAT TGAGCTGTCT 
CCACAGGATC AGAAGCTGGA CATCATCAAG GTCACTGGTG CCAGAGCGAA TATCGAAGAA 
GCCAAGATTG CAATTGCCAA GCGAATCGAA GAGCTCGAGG CTGATCGCAA GGATCGTGAA 
CTGCGTTCAT TTGAAGTCAA GGTCGAAATC GATCCGGAAT ACCATCCCAA GATCATCGGT 
CGTCGTGGTT TCGTCGTTAA TAAGATTCGT GCCACCCATG GAGTGCAGAT TTCGTTCCCG 
AAGCAAGATG ACCCTCAGAA CAACATCATC ACCATCCAAG GATACGAAAG TAACGCCCAT 
GCTGCCAAGG ATGAAATCTT AGGAATTGTC AACGAGCTTA ACTCGATGCA CCGCGAGGAA 
GTATCCATTG ATGAACGCGT CCACCGTCGT TTCATCGGCT TCAAGGGACG TCGTGTCAAG 
GAGATCAAGG AAATGTTCAA GGTGGAAATC AGCTTCCCAC GTCCGGAAGA TAAGGATCAG 
TCGCTTATCG TTATTGCTGG TAACAATCAG GACGACGTTG AAGCTTGTCG TGATCATCTG 
CTCAACTTGG AGGAAGAATT CCTTCAGGAC GTTGTTACCG CTGCACCACC GCAGCAAACC 
ACTTTCACCC AGTTCCTGGA GGACTCTATG AGCAATCGCG AGGCCACCAA GAAGGGCTTT 
GTCGTTCAAG GTGCCCCATG GGAACGCAAG ACTCCCAACA CTCAATCGCA GGAGGACTTC 
CCAGATTTCG GTCTTGGATC GGCTCCAGCT GAACAAGCTC CCCAGGCTCC AATCGCTTCG 
GCCTGGAATG CGCGCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001659185.1
CDS	219..3959
Translation	MEDSTVVDQQPSNSAYENNVASTPAPTPCYDDLFPALPESAPPRFNNPTLSAATQSMRVGSSVVTQVFIVPSGERKYDSDKFGEGESLRTCQNIMKETNAHIEISSGKDQSLTFLVTGKPNEVIEARRKILVHFQTQASKSISIPREHHRWILGKKGDRLRDLEKNTATKIVVPRINDESDIITISGTKEGIEKAEHEIRTTSDEQSRKAFERVNIPKIYHPFIMGPHNDNVSKMMEETGAKINIPPPSVQKDEIIITGEKEGVLQAKARIEAISKEMEKKCTSVGVEVPRAQHKYIVGPKGSTIQEILAMTGVSVEMPASDSPNDTITLRGPQDKLGNALSVVYQKANSVRTTTLECPQWIHKYIIGREGGHIKEFTAQYPNVHVEFNEDKIKIEGLPEQVDASSEALEKMVKDYTGRLTFTEMVVDPIYCKHIIGKNGANINRMKEEYEVLINIDEKDLKPIRIEGPADGVEKAKQELLEKIAKLENEKEESIVIDHRLFKTIIGAKGETIREIRDKHNQVQIIFPGATDKSDIVKIRGPRDDVDKCHKYLTKYVKELQKNSFVMEVPIFKQFHKYIIGKGGANIKKIRDETQTKIDLPAEGDKNEMIVITGKKDNVKEARNRILKIQEELSNIITEDIQIPPKYYNSLIGSGGKLISSIMEECGGVSIKFPSPESNSDKVAIRGPKEDVERAKQQLLELTNEKELSSFSAEVRAKPQHHKFLIGKNGASIKKIRDQTGARIVFPGYNDEDKEAIIIIGKKEGVEEAKAQLESIIKNIDNIVEDEISVEPRHHKHFVARRGEVLHRISEECGGVMISFPRSGVDSDRVTLKGAKECIEAAKARILEIVDDLEQMVTIECIIPAHNHRIVMGKGGSKVQGITSEFDVNIKFPERDAPEAFVEGQQNGDANGAEPIKTTDIIKISGRKEKCEKAKEALLALVPVTEEINVAFDLHRSLIGQKGRDVKELMNTYDVHIELSPQDQKLDIIKVTGARANIEEAKIAIAKRIEELEADRKDRELRSFEVKVEIDPEYHPKIIGRRGFVVNKIRATHGVQISFPKQDDPQNNIITIQGYESNAHAAKDEILGIVNELNSMHREEVSIDERVHRRFIGFKGRRVKEIKEMFKVEISFPRPEDKDQSLIVIAGNNQDDVEACRDHLLNLEEEFLQDVVTAAPPQQTTFTQFLEDSMSNREATKKGFVVQGAPWERKTPNTQSQEDFPDFGLGSAPAEQAPQAPIASAWNARH

Target ORF information:

RefSeq Version	XM_001659135.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL001421-RA mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001659135.1

ORF Insert Sequence:

ATGGAGGATT CAACAGTAGT TGACCAGCAG CCCAGCAACT CCGCGTACGA GAACAACGTT 
GCCTCGACGC CGGCTCCGAC CCCGTGCTAC GATGATCTGT TCCCGGCCCT GCCGGAGAGC 
GCCCCACCGC GCTTCAACAA TCCGACCCTG TCGGCGGCCA CGCAGAGTAT GCGCGTCGGC 
AGCTCGGTCG TGACGCAGGT CTTCATCGTG CCGTCCGGCG AGCGCAAGTA CGACTCGGAC 
AAGTTTGGCG AGGGCGAATC GTTGCGCACC TGCCAGAACA TCATGAAGGA AACCAACGCC 
CATATCGAGA TCTCGAGTGG CAAGGACCAA TCGTTGACCT TCCTGGTGAC TGGCAAGCCG 
AACGAGGTGA TCGAGGCCCG CCGTAAGATT TTGGTGCACT TCCAAACTCA GGCGAGCAAG 
TCGATCAGCA TTCCGCGGGA GCACCACCGC TGGATCCTCG GCAAGAAGGG CGACCGGCTG 
CGCGATCTGG AGAAGAACAC CGCCACCAAG ATCGTTGTGC CGCGCATCAA CGACGAGTCG 
GACATCATCA CCATCTCCGG TACCAAGGAA GGCATCGAGA AGGCCGAACA TGAAATCCGT 
ACCACGTCTG ATGAGCAGTC GCGTAAGGCC TTCGAGCGGG TTAACATCCC CAAGATTTAC 
CATCCGTTCA TCATGGGACC CCACAACGAC AACGTCAGCA AGATGATGGA GGAAACCGGA 
GCGAAGATCA ACATTCCGCC GCCGTCCGTC CAAAAGGATG AGATCATCAT CACCGGCGAG 
AAGGAAGGTG TACTCCAGGC AAAGGCACGC ATCGAAGCCA TCAGTAAGGA GATGGAAAAG 
AAGTGTACCA GCGTCGGCGT TGAGGTTCCC CGTGCTCAGC ATAAGTACAT CGTTGGTCCC 
AAGGGTTCCA CCATCCAGGA AATCTTGGCG ATGACCGGTG TATCGGTTGA GATGCCGGCT 
AGTGACTCTC CCAACGATAC CATCACTCTG CGCGGCCCCC AGGACAAGCT CGGAAATGCT 
TTGAGCGTTG TGTACCAGAA GGCGAACTCT GTTCGCACGA CTACGCTCGA GTGTCCCCAG 
TGGATTCATA AGTATATCAT TGGACGCGAG GGTGGCCATA TCAAGGAATT CACCGCTCAG 
TATCCCAACG TACACGTGGA GTTCAACGAA GACAAGATCA AGATCGAAGG CCTACCGGAA 
CAGGTGGATG CTTCTTCGGA AGCGCTGGAG AAAATGGTCA AGGATTACAC TGGACGTCTA 
ACCTTCACCG AAATGGTGGT TGATCCGATC TACTGCAAGC ACATCATCGG TAAGAACGGA 
GCCAACATCA ACCGTATGAA GGAGGAATAC GAGGTCTTGA TCAACATCGA CGAGAAGGAC 
TTGAAACCGA TCCGCATCGA AGGACCGGCC GACGGTGTTG AGAAGGCCAA GCAGGAGCTG 
CTTGAAAAGA TAGCGAAACT GGAAAACGAA AAGGAAGAGT CGATCGTGAT CGATCATCGT 
CTGTTCAAGA CGATCATCGG AGCGAAAGGC GAAACGATTC GGGAAATCCG TGACAAGCAT 
AACCAGGTGC AGATTATCTT CCCCGGAGCT ACCGACAAGT CGGACATTGT CAAGATCCGT 
GGTCCGCGTG ACGATGTGGA TAAGTGTCAC AAGTATCTGA CGAAGTACGT TAAGGAGCTG 
CAGAAGAACT CCTTCGTAAT GGAAGTCCCA ATCTTCAAGC AATTCCACAA GTACATTATC 
GGCAAGGGTG GTGCCAACAT CAAGAAGATT CGTGATGAGA CTCAAACGAA AATCGATCTT 
CCAGCAGAGG GTGACAAAAA CGAGATGATT GTCATCACTG GCAAGAAGGA CAACGTTAAG 
GAGGCTCGCA ATCGCATTCT GAAGATCCAG GAGGAACTTT CCAACATCAT CACCGAGGAT 
ATTCAGATCC CACCCAAGTA CTACAATTCG TTGATCGGAT CTGGTGGCAA GCTGATTTCC 
TCGATTATGG AAGAGTGCGG TGGGGTTTCG ATCAAGTTCC CAAGTCCGGA GTCCAACAGC 
GACAAGGTGG CCATCCGTGG CCCGAAGGAA GATGTCGAAC GAGCTAAGCA ACAGCTGCTC 
GAGCTGACCA ATGAGAAGGA GCTTTCTTCC TTCTCTGCCG AGGTTCGTGC CAAACCGCAG 
CACCATAAGT TCCTCATCGG TAAGAACGGC GCTTCCATCA AGAAGATCCG GGACCAAACC 
GGTGCTCGTA TTGTCTTCCC GGGTTACAAC GACGAGGACA AGGAGGCCAT CATCATCATC 
GGCAAGAAGG AAGGTGTCGA AGAGGCCAAG GCCCAGTTGG AGTCGATTAT CAAGAACATC 
GATAACATTG TTGAAGATGA GATTAGCGTG GAGCCGAGGC ACCATAAGCA TTTCGTGGCT 
CGCCGTGGCG AGGTGCTGCA CCGTATTTCG GAGGAATGCG GTGGCGTGAT GATTTCCTTC 
CCCCGCTCGG GAGTGGACAG CGATCGGGTT ACGCTGAAGG GTGCCAAGGA GTGCATTGAA 
GCGGCCAAGG CTCGCATCCT GGAGATCGTC GACGATTTGG AGCAGATGGT TACGATCGAG 
TGTATCATTC CGGCTCATAA CCATCGCATT GTTATGGGTA AAGGTGGTTC CAAGGTGCAA 
GGAATTACAT CGGAGTTTGA TGTGAACATT AAGTTCCCGG AACGAGACGC GCCGGAAGCG 
TTCGTTGAAG GGCAACAAAA TGGCGATGCA AATGGAGCGG AGCCCATTAA GACGACCGAT 
ATTATCAAGA TTAGTGGTCG CAAAGAAAAG TGCGAAAAGG CAAAGGAAGC TTTGTTGGCT 
TTGGTCCCGG TCACTGAGGA GATTAACGTT GCATTTGATC TGCACAGATC GTTGATTGGC 
CAGAAGGGTC GTGATGTCAA GGAGTTGATG AATACGTACG ATGTTCACAT TGAGCTGTCT 
CCACAGGATC AGAAGCTGGA CATCATCAAG GTCACTGGTG CCAGAGCGAA TATCGAAGAA 
GCCAAGATTG CAATTGCCAA GCGAATCGAA GAGCTCGAGG CTGATCGCAA GGATCGTGAA 
CTGCGTTCAT TTGAAGTCAA GGTCGAAATC GATCCGGAAT ACCATCCCAA GATCATCGGT 
CGTCGTGGTT TCGTCGTTAA TAAGATTCGT GCCACCCATG GAGTGCAGAT TTCGTTCCCG 
AAGCAAGATG ACCCTCAGAA CAACATCATC ACCATCCAAG GATACGAAAG TAACGCCCAT 
GCTGCCAAGG ATGAAATCTT AGGAATTGTC AACGAGCTTA ACTCGATGCA CCGCGAGGAA 
GTATCCATTG ATGAACGCGT CCACCGTCGT TTCATCGGCT TCAAGGGACG TCGTGTCAAG 
GAGATCAAGG AAATGTTCAA GGTGGAAATC AGCTTCCCAC GTCCGGAAGA TAAGGATCAG 
TCGCTTATCG TTATTGCTGG TAACAATCAG GACGACGTTG AAGCTTGTCG TGATCATCTG 
CTCAACTTGG AGGAAGAATT CCTTCAGGAC GTTGTTACCG CTGCACCACC GCAGCAAACC 
ACTTTCACCC AGTTCCTGGA GGACTCTATG AGCAATCGCG AGGCCACCAA GAAGGGCTTT 
GTCGTTCAAG GTGCCCCATG GGAACGCAAG ACTCCCAACA CTCAATCGCA GGAGGACTTC 
CCAGATTTCG GTCTTGGATC GGCTCCAGCT GAACAAGCTC CCCAGGCTCC AATCGCTTCG 
GCCTGGAATG CGCGCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5570534 ( XM_001659135.2 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001659185.1 Aedes aegypti vigilin (LOC5570534), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAe119094
Clone ID Related Accession (Same CDS sequence)	XM_001659135.2 , XM_021840756.1 , XM_021840757.1
Accession Version	XM_001659135.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	vigilin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 20, 2017 this sequence version replaced XM_001659135.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATT CAACAGTAGT TGACCAGCAG CCCAGCAACT CCGCGTACGA GAACAACGTT 
GCCTCGACGC CGGCTCCGAC CCCGTGCTAC GATGATCTGT TCCCGGCCCT GCCGGAGAGC 
GCCCCACCGC GCTTCAACAA TCCGACCCTG TCGGCGGCCA CGCAGAGTAT GCGCGTCGGC 
AGCTCGGTCG TGACGCAGGT CTTCATCGTG CCGTCCGGCG AGCGCAAGTA CGACTCGGAC 
AAGTTTGGCG AGGGCGAATC GTTGCGCACC TGCCAGAACA TCATGAAGGA AACCAACGCC 
CATATCGAGA TCTCGAGTGG CAAGGACCAA TCGTTGACCT TCCTGGTGAC TGGCAAGCCG 
AACGAGGTGA TCGAGGCCCG CCGTAAGATT TTGGTGCACT TCCAAACTCA GGCGAGCAAG 
TCGATCAGCA TTCCGCGGGA GCACCACCGC TGGATCCTCG GCAAGAAGGG CGACCGGCTG 
CGCGATCTGG AGAAGAACAC CGCCACCAAG ATCGTTGTGC CGCGCATCAA CGACGAGTCG 
GACATCATCA CCATCTCCGG TACCAAGGAA GGCATCGAGA AGGCCGAACA TGAAATCCGT 
ACCACGTCTG ATGAGCAGTC GCGTAAGGCC TTCGAGCGGG TTAACATCCC CAAGATTTAC 
CATCCGTTCA TCATGGGACC CCACAACGAC AACGTCAGCA AGATGATGGA GGAAACCGGA 
GCGAAGATCA ACATTCCGCC GCCGTCCGTC CAAAAGGATG AGATCATCAT CACCGGCGAG 
AAGGAAGGTG TACTCCAGGC AAAGGCACGC ATCGAAGCCA TCAGTAAGGA GATGGAAAAG 
AAGTGTACCA GCGTCGGCGT TGAGGTTCCC CGTGCTCAGC ATAAGTACAT CGTTGGTCCC 
AAGGGTTCCA CCATCCAGGA AATCTTGGCG ATGACCGGTG TATCGGTTGA GATGCCGGCT 
AGTGACTCTC CCAACGATAC CATCACTCTG CGCGGCCCCC AGGACAAGCT CGGAAATGCT 
TTGAGCGTTG TGTACCAGAA GGCGAACTCT GTTCGCACGA CTACGCTCGA GTGTCCCCAG 
TGGATTCATA AGTATATCAT TGGACGCGAG GGTGGCCATA TCAAGGAATT CACCGCTCAG 
TATCCCAACG TACACGTGGA GTTCAACGAA GACAAGATCA AGATCGAAGG CCTACCGGAA 
CAGGTGGATG CTTCTTCGGA AGCGCTGGAG AAAATGGTCA AGGATTACAC TGGACGTCTA 
ACCTTCACCG AAATGGTGGT TGATCCGATC TACTGCAAGC ACATCATCGG TAAGAACGGA 
GCCAACATCA ACCGTATGAA GGAGGAATAC GAGGTCTTGA TCAACATCGA CGAGAAGGAC 
TTGAAACCGA TCCGCATCGA AGGACCGGCC GACGGTGTTG AGAAGGCCAA GCAGGAGCTG 
CTTGAAAAGA TAGCGAAACT GGAAAACGAA AAGGAAGAGT CGATCGTGAT CGATCATCGT 
CTGTTCAAGA CGATCATCGG AGCGAAAGGC GAAACGATTC GGGAAATCCG TGACAAGCAT 
AACCAGGTGC AGATTATCTT CCCCGGAGCT ACCGACAAGT CGGACATTGT CAAGATCCGT 
GGTCCGCGTG ACGATGTGGA TAAGTGTCAC AAGTATCTGA CGAAGTACGT TAAGGAGCTG 
CAGAAGAACT CCTTCGTAAT GGAAGTCCCA ATCTTCAAGC AATTCCACAA GTACATTATC 
GGCAAGGGTG GTGCCAACAT CAAGAAGATT CGTGATGAGA CTCAAACGAA AATCGATCTT 
CCAGCAGAGG GTGACAAGAA CGAGATGATT GTCATCACTG GCAAGAAGGA CAACGTTAAG 
GAGGCTCGCA ATCGCATTCT GAAGATCCAG GAGGAACTTT CCAACATCAT CACCGAGGAT 
ATTCAGATCC CACCCAAGTA CTACAATTCG TTGATCGGAT CTGGTGGCAA GCTGATTTCC 
TCGATTATGG AAGAGTGCGG TGGGGTTTCG ATCAAGTTCC CAAGTCCGGA GTCCAACAGC 
GACAAGGTGG CCATCCGTGG CCCGAAGGAA GATGTCGAAC GAGCTAAGCA ACAGCTGCTC 
GAGCTGACCA ATGAGAAGGA GCTCTCTTCC TTCTCTGCCG AGGTTCGTGC CAAACCGCAG 
CACCATAAGT TCCTCATCGG TAAGAACGGC GCTTCCATCA AGAAGATCCG GGACCAAACC 
GGTGCTCGTA TTGTCTTCCC GGGTTACAAC GACGAGGACA AGGAGGCCAT CATCATCATC 
GGCAAGAAGG AAGGTGTCGA AGAGGCCAAG GCCCAGTTGG AGTCGATTAT CAAGAACATC 
GATAACATTG TTGAAGATGA GATTAGCGTG GAGCCGAGGC ACCATAAGCA TTTCGTGGCT 
CGCCGTGGCG AGGTGCTGCA CCGTATTTCG GAGGAGTGCG GTGGCGTGAT GATTTCCTTC 
CCCCGCTCGG GAGTGGACAG CGATCGGGTT ACGCTGAAGG GTGCCAAGGA GTGCATTGAG 
GCGGCCAAGG CTCGCATCCT GGAGATCGTC GATGATTTGG AGCAGATGGT TACGATCGAG 
TGTATCATTC CGGCTCATAA CCATCGCATT GTTATGGGTA AGGGTGGTTC CAAGGTGCAA 
GGAATTACAT CGGAGTTTGA TGTGAACATT AAGTTCCCGG AACGAGACGC GCCGGAAGCG 
TTCGTTGAAG GGCAACAAAA TGGCGATGCA AATGGAGCGG AGCCCATTAA GACGACCGAT 
ATTATCAAGA TTAGTGGTCG CAAAGAGAAG TGCGAAAAGG CAAAGGAAGC TTTGTTGGCT 
TTGGTCCCGG TCACTGAGGA GATTAACGTT GCATTTGATC TGCACAGATC GTTGATTGGC 
CAGAAGGGTC GTGATGTCAA GGAGTTGATG AATACGTACG ATGTCCACAT TGAGCTGTCT 
CCACAGGATC AGAAGCTGGA CATCATCAAG GTCACTGGTG CCAGAGCGAA TATCGAAGAA 
GCCAAGATTG CAATTGCCAA GCGAATCGAA GAGCTCGAGG CTGATCGCAA GGATCGTGAA 
CTGCGTTCAT TTGAAGTCAA GGTCGAAATC GATCCGGAAT ACCATCCCAA GATCATCGGT 
CGTCGTGGTT TCGTCGTTAA TAAGATTCGT GCCACCCATG GAGTGCAGAT TTCGTTCCCG 
AAGCAAGATG ACCCTCAGAA CAACATCATC ACCATCCAAG GATACGAAAG TAACGCCCAT 
GCTGCCAAGG ATGAAATCTT AGGAATTGTC AACGAGCTTA ACTCGATGCA CCGCGAGGAA 
GTATCCATTG ATGAACGCGT CCACCGTCGT TTCATCGGCT TCAAGGGACG TCGTGTCAAG 
GAGATCAAGG AAATGTTCAA GGTGGAAATC AGCTTCCCAC GTCCGGAAGA TAAGGATCAG 
TCGCTTATCG TTATTGCTGG TAACAATCAG GACGACGTTG AAGCTTGTCG TGATCATCTG 
CTCAACTTGG AGGAAGAATT CCTCCAGGAC GTTGTTACCG CTGCACCACC GCAGCAAACC 
ACTTTCACCC AGTTCCTGGA GGACTCAATG AGCAATCGCG AGGCCACCAA GAAGGGCTTT 
GTCGTTCAAG GTGCCCCATG GGAACGCAAG ACTCCCAACA CTCAATCGCA GGAGGACTTC 
CCAGATTTCG GTCTTGGATC GGCTCCAGCT GAACAAGCTC CCCAGGCTCC AATCGCTTCG 
GCCTGGAATG CGCGCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001659185.1
CDS	259..3999
Translation	MEDSTVVDQQPSNSAYENNVASTPAPTPCYDDLFPALPESAPPRFNNPTLSAATQSMRVGSSVVTQVFIVPSGERKYDSDKFGEGESLRTCQNIMKETNAHIEISSGKDQSLTFLVTGKPNEVIEARRKILVHFQTQASKSISIPREHHRWILGKKGDRLRDLEKNTATKIVVPRINDESDIITISGTKEGIEKAEHEIRTTSDEQSRKAFERVNIPKIYHPFIMGPHNDNVSKMMEETGAKINIPPPSVQKDEIIITGEKEGVLQAKARIEAISKEMEKKCTSVGVEVPRAQHKYIVGPKGSTIQEILAMTGVSVEMPASDSPNDTITLRGPQDKLGNALSVVYQKANSVRTTTLECPQWIHKYIIGREGGHIKEFTAQYPNVHVEFNEDKIKIEGLPEQVDASSEALEKMVKDYTGRLTFTEMVVDPIYCKHIIGKNGANINRMKEEYEVLINIDEKDLKPIRIEGPADGVEKAKQELLEKIAKLENEKEESIVIDHRLFKTIIGAKGETIREIRDKHNQVQIIFPGATDKSDIVKIRGPRDDVDKCHKYLTKYVKELQKNSFVMEVPIFKQFHKYIIGKGGANIKKIRDETQTKIDLPAEGDKNEMIVITGKKDNVKEARNRILKIQEELSNIITEDIQIPPKYYNSLIGSGGKLISSIMEECGGVSIKFPSPESNSDKVAIRGPKEDVERAKQQLLELTNEKELSSFSAEVRAKPQHHKFLIGKNGASIKKIRDQTGARIVFPGYNDEDKEAIIIIGKKEGVEEAKAQLESIIKNIDNIVEDEISVEPRHHKHFVARRGEVLHRISEECGGVMISFPRSGVDSDRVTLKGAKECIEAAKARILEIVDDLEQMVTIECIIPAHNHRIVMGKGGSKVQGITSEFDVNIKFPERDAPEAFVEGQQNGDANGAEPIKTTDIIKISGRKEKCEKAKEALLALVPVTEEINVAFDLHRSLIGQKGRDVKELMNTYDVHIELSPQDQKLDIIKVTGARANIEEAKIAIAKRIEELEADRKDRELRSFEVKVEIDPEYHPKIIGRRGFVVNKIRATHGVQISFPKQDDPQNNIITIQGYESNAHAAKDEILGIVNELNSMHREEVSIDERVHRRFIGFKGRRVKEIKEMFKVEISFPRPEDKDQSLIVIAGNNQDDVEACRDHLLNLEEEFLQDVVTAAPPQQTTFTQFLEDSMSNREATKKGFVVQGAPWERKTPNTQSQEDFPDFGLGSAPAEQAPQAPIASAWNARH

Target ORF information:

RefSeq Version	XM_001659135.2
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti vigilin (LOC5570534), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001659135.2

ORF Insert Sequence:

ATGGAGGATT CAACAGTAGT TGACCAGCAG CCCAGCAACT CCGCGTACGA GAACAACGTT 
GCCTCGACGC CGGCTCCGAC CCCGTGCTAC GATGATCTGT TCCCGGCCCT GCCGGAGAGC 
GCCCCACCGC GCTTCAACAA TCCGACCCTG TCGGCGGCCA CGCAGAGTAT GCGCGTCGGC 
AGCTCGGTCG TGACGCAGGT CTTCATCGTG CCGTCCGGCG AGCGCAAGTA CGACTCGGAC 
AAGTTTGGCG AGGGCGAATC GTTGCGCACC TGCCAGAACA TCATGAAGGA AACCAACGCC 
CATATCGAGA TCTCGAGTGG CAAGGACCAA TCGTTGACCT TCCTGGTGAC TGGCAAGCCG 
AACGAGGTGA TCGAGGCCCG CCGTAAGATT TTGGTGCACT TCCAAACTCA GGCGAGCAAG 
TCGATCAGCA TTCCGCGGGA GCACCACCGC TGGATCCTCG GCAAGAAGGG CGACCGGCTG 
CGCGATCTGG AGAAGAACAC CGCCACCAAG ATCGTTGTGC CGCGCATCAA CGACGAGTCG 
GACATCATCA CCATCTCCGG TACCAAGGAA GGCATCGAGA AGGCCGAACA TGAAATCCGT 
ACCACGTCTG ATGAGCAGTC GCGTAAGGCC TTCGAGCGGG TTAACATCCC CAAGATTTAC 
CATCCGTTCA TCATGGGACC CCACAACGAC AACGTCAGCA AGATGATGGA GGAAACCGGA 
GCGAAGATCA ACATTCCGCC GCCGTCCGTC CAAAAGGATG AGATCATCAT CACCGGCGAG 
AAGGAAGGTG TACTCCAGGC AAAGGCACGC ATCGAAGCCA TCAGTAAGGA GATGGAAAAG 
AAGTGTACCA GCGTCGGCGT TGAGGTTCCC CGTGCTCAGC ATAAGTACAT CGTTGGTCCC 
AAGGGTTCCA CCATCCAGGA AATCTTGGCG ATGACCGGTG TATCGGTTGA GATGCCGGCT 
AGTGACTCTC CCAACGATAC CATCACTCTG CGCGGCCCCC AGGACAAGCT CGGAAATGCT 
TTGAGCGTTG TGTACCAGAA GGCGAACTCT GTTCGCACGA CTACGCTCGA GTGTCCCCAG 
TGGATTCATA AGTATATCAT TGGACGCGAG GGTGGCCATA TCAAGGAATT CACCGCTCAG 
TATCCCAACG TACACGTGGA GTTCAACGAA GACAAGATCA AGATCGAAGG CCTACCGGAA 
CAGGTGGATG CTTCTTCGGA AGCGCTGGAG AAAATGGTCA AGGATTACAC TGGACGTCTA 
ACCTTCACCG AAATGGTGGT TGATCCGATC TACTGCAAGC ACATCATCGG TAAGAACGGA 
GCCAACATCA ACCGTATGAA GGAGGAATAC GAGGTCTTGA TCAACATCGA CGAGAAGGAC 
TTGAAACCGA TCCGCATCGA AGGACCGGCC GACGGTGTTG AGAAGGCCAA GCAGGAGCTG 
CTTGAAAAGA TAGCGAAACT GGAAAACGAA AAGGAAGAGT CGATCGTGAT CGATCATCGT 
CTGTTCAAGA CGATCATCGG AGCGAAAGGC GAAACGATTC GGGAAATCCG TGACAAGCAT 
AACCAGGTGC AGATTATCTT CCCCGGAGCT ACCGACAAGT CGGACATTGT CAAGATCCGT 
GGTCCGCGTG ACGATGTGGA TAAGTGTCAC AAGTATCTGA CGAAGTACGT TAAGGAGCTG 
CAGAAGAACT CCTTCGTAAT GGAAGTCCCA ATCTTCAAGC AATTCCACAA GTACATTATC 
GGCAAGGGTG GTGCCAACAT CAAGAAGATT CGTGATGAGA CTCAAACGAA AATCGATCTT 
CCAGCAGAGG GTGACAAGAA CGAGATGATT GTCATCACTG GCAAGAAGGA CAACGTTAAG 
GAGGCTCGCA ATCGCATTCT GAAGATCCAG GAGGAACTTT CCAACATCAT CACCGAGGAT 
ATTCAGATCC CACCCAAGTA CTACAATTCG TTGATCGGAT CTGGTGGCAA GCTGATTTCC 
TCGATTATGG AAGAGTGCGG TGGGGTTTCG ATCAAGTTCC CAAGTCCGGA GTCCAACAGC 
GACAAGGTGG CCATCCGTGG CCCGAAGGAA GATGTCGAAC GAGCTAAGCA ACAGCTGCTC 
GAGCTGACCA ATGAGAAGGA GCTCTCTTCC TTCTCTGCCG AGGTTCGTGC CAAACCGCAG 
CACCATAAGT TCCTCATCGG TAAGAACGGC GCTTCCATCA AGAAGATCCG GGACCAAACC 
GGTGCTCGTA TTGTCTTCCC GGGTTACAAC GACGAGGACA AGGAGGCCAT CATCATCATC 
GGCAAGAAGG AAGGTGTCGA AGAGGCCAAG GCCCAGTTGG AGTCGATTAT CAAGAACATC 
GATAACATTG TTGAAGATGA GATTAGCGTG GAGCCGAGGC ACCATAAGCA TTTCGTGGCT 
CGCCGTGGCG AGGTGCTGCA CCGTATTTCG GAGGAGTGCG GTGGCGTGAT GATTTCCTTC 
CCCCGCTCGG GAGTGGACAG CGATCGGGTT ACGCTGAAGG GTGCCAAGGA GTGCATTGAG 
GCGGCCAAGG CTCGCATCCT GGAGATCGTC GATGATTTGG AGCAGATGGT TACGATCGAG 
TGTATCATTC CGGCTCATAA CCATCGCATT GTTATGGGTA AGGGTGGTTC CAAGGTGCAA 
GGAATTACAT CGGAGTTTGA TGTGAACATT AAGTTCCCGG AACGAGACGC GCCGGAAGCG 
TTCGTTGAAG GGCAACAAAA TGGCGATGCA AATGGAGCGG AGCCCATTAA GACGACCGAT 
ATTATCAAGA TTAGTGGTCG CAAAGAGAAG TGCGAAAAGG CAAAGGAAGC TTTGTTGGCT 
TTGGTCCCGG TCACTGAGGA GATTAACGTT GCATTTGATC TGCACAGATC GTTGATTGGC 
CAGAAGGGTC GTGATGTCAA GGAGTTGATG AATACGTACG ATGTCCACAT TGAGCTGTCT 
CCACAGGATC AGAAGCTGGA CATCATCAAG GTCACTGGTG CCAGAGCGAA TATCGAAGAA 
GCCAAGATTG CAATTGCCAA GCGAATCGAA GAGCTCGAGG CTGATCGCAA GGATCGTGAA 
CTGCGTTCAT TTGAAGTCAA GGTCGAAATC GATCCGGAAT ACCATCCCAA GATCATCGGT 
CGTCGTGGTT TCGTCGTTAA TAAGATTCGT GCCACCCATG GAGTGCAGAT TTCGTTCCCG 
AAGCAAGATG ACCCTCAGAA CAACATCATC ACCATCCAAG GATACGAAAG TAACGCCCAT 
GCTGCCAAGG ATGAAATCTT AGGAATTGTC AACGAGCTTA ACTCGATGCA CCGCGAGGAA 
GTATCCATTG ATGAACGCGT CCACCGTCGT TTCATCGGCT TCAAGGGACG TCGTGTCAAG 
GAGATCAAGG AAATGTTCAA GGTGGAAATC AGCTTCCCAC GTCCGGAAGA TAAGGATCAG 
TCGCTTATCG TTATTGCTGG TAACAATCAG GACGACGTTG AAGCTTGTCG TGATCATCTG 
CTCAACTTGG AGGAAGAATT CCTCCAGGAC GTTGTTACCG CTGCACCACC GCAGCAAACC 
ACTTTCACCC AGTTCCTGGA GGACTCAATG AGCAATCGCG AGGCCACCAA GAAGGGCTTT 
GTCGTTCAAG GTGCCCCATG GGAACGCAAG ACTCCCAACA CTCAATCGCA GGAGGACTTC 
CCAGATTTCG GTCTTGGATC GGCTCCAGCT GAACAAGCTC CCCAGGCTCC AATCGCTTCG 
GCCTGGAATG CGCGCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5570534 ( XM_021840756.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021696448.1 Aedes aegypti vigilin (LOC5570534), transcript variant X2, mRNA.

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CloneID	OAe119094
Clone ID Related Accession (Same CDS sequence)	XM_001659135.2 , XM_021840756.1 , XM_021840757.1
Accession Version	XM_021840756.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	vigilin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATT CAACAGTAGT TGACCAGCAG CCCAGCAACT CCGCGTACGA GAACAACGTT 
GCCTCGACGC CGGCTCCGAC CCCGTGCTAC GATGATCTGT TCCCGGCCCT GCCGGAGAGC 
GCCCCACCGC GCTTCAACAA TCCGACCCTG TCGGCGGCCA CGCAGAGTAT GCGCGTCGGC 
AGCTCGGTCG TGACGCAGGT CTTCATCGTG CCGTCCGGCG AGCGCAAGTA CGACTCGGAC 
AAGTTTGGCG AGGGCGAATC GTTGCGCACC TGCCAGAACA TCATGAAGGA AACCAACGCC 
CATATCGAGA TCTCGAGTGG CAAGGACCAA TCGTTGACCT TCCTGGTGAC TGGCAAGCCG 
AACGAGGTGA TCGAGGCCCG CCGTAAGATT TTGGTGCACT TCCAAACTCA GGCGAGCAAG 
TCGATCAGCA TTCCGCGGGA GCACCACCGC TGGATCCTCG GCAAGAAGGG CGACCGGCTG 
CGCGATCTGG AGAAGAACAC CGCCACCAAG ATCGTTGTGC CGCGCATCAA CGACGAGTCG 
GACATCATCA CCATCTCCGG TACCAAGGAA GGCATCGAGA AGGCCGAACA TGAAATCCGT 
ACCACGTCTG ATGAGCAGTC GCGTAAGGCC TTCGAGCGGG TTAACATCCC CAAGATTTAC 
CATCCGTTCA TCATGGGACC CCACAACGAC AACGTCAGCA AGATGATGGA GGAAACCGGA 
GCGAAGATCA ACATTCCGCC GCCGTCCGTC CAAAAGGATG AGATCATCAT CACCGGCGAG 
AAGGAAGGTG TACTCCAGGC AAAGGCACGC ATCGAAGCCA TCAGTAAGGA GATGGAAAAG 
AAGTGTACCA GCGTCGGCGT TGAGGTTCCC CGTGCTCAGC ATAAGTACAT CGTTGGTCCC 
AAGGGTTCCA CCATCCAGGA AATCTTGGCG ATGACCGGTG TATCGGTTGA GATGCCGGCT 
AGTGACTCTC CCAACGATAC CATCACTCTG CGCGGCCCCC AGGACAAGCT CGGAAATGCT 
TTGAGCGTTG TGTACCAGAA GGCGAACTCT GTTCGCACGA CTACGCTCGA GTGTCCCCAG 
TGGATTCATA AGTATATCAT TGGACGCGAG GGTGGCCATA TCAAGGAATT CACCGCTCAG 
TATCCCAACG TACACGTGGA GTTCAACGAA GACAAGATCA AGATCGAAGG CCTACCGGAA 
CAGGTGGATG CTTCTTCGGA AGCGCTGGAG AAAATGGTCA AGGATTACAC TGGACGTCTA 
ACCTTCACCG AAATGGTGGT TGATCCGATC TACTGCAAGC ACATCATCGG TAAGAACGGA 
GCCAACATCA ACCGTATGAA GGAGGAATAC GAGGTCTTGA TCAACATCGA CGAGAAGGAC 
TTGAAACCGA TCCGCATCGA AGGACCGGCC GACGGTGTTG AGAAGGCCAA GCAGGAGCTG 
CTTGAAAAGA TAGCGAAACT GGAAAACGAA AAGGAAGAGT CGATCGTGAT CGATCATCGT 
CTGTTCAAGA CGATCATCGG AGCGAAAGGC GAAACGATTC GGGAAATCCG TGACAAGCAT 
AACCAGGTGC AGATTATCTT CCCCGGAGCT ACCGACAAGT CGGACATTGT CAAGATCCGT 
GGTCCGCGTG ACGATGTGGA TAAGTGTCAC AAGTATCTGA CGAAGTACGT TAAGGAGCTG 
CAGAAGAACT CCTTCGTAAT GGAAGTCCCA ATCTTCAAGC AATTCCACAA GTACATTATC 
GGCAAGGGTG GTGCCAACAT CAAGAAGATT CGTGATGAGA CTCAAACGAA AATCGATCTT 
CCAGCAGAGG GTGACAAGAA CGAGATGATT GTCATCACTG GCAAGAAGGA CAACGTTAAG 
GAGGCTCGCA ATCGCATTCT GAAGATCCAG GAGGAACTTT CCAACATCAT CACCGAGGAT 
ATTCAGATCC CACCCAAGTA CTACAATTCG TTGATCGGAT CTGGTGGCAA GCTGATTTCC 
TCGATTATGG AAGAGTGCGG TGGGGTTTCG ATCAAGTTCC CAAGTCCGGA GTCCAACAGC 
GACAAGGTGG CCATCCGTGG CCCGAAGGAA GATGTCGAAC GAGCTAAGCA ACAGCTGCTC 
GAGCTGACCA ATGAGAAGGA GCTCTCTTCC TTCTCTGCCG AGGTTCGTGC CAAACCGCAG 
CACCATAAGT TCCTCATCGG TAAGAACGGC GCTTCCATCA AGAAGATCCG GGACCAAACC 
GGTGCTCGTA TTGTCTTCCC GGGTTACAAC GACGAGGACA AGGAGGCCAT CATCATCATC 
GGCAAGAAGG AAGGTGTCGA AGAGGCCAAG GCCCAGTTGG AGTCGATTAT CAAGAACATC 
GATAACATTG TTGAAGATGA GATTAGCGTG GAGCCGAGGC ACCATAAGCA TTTCGTGGCT 
CGCCGTGGCG AGGTGCTGCA CCGTATTTCG GAGGAGTGCG GTGGCGTGAT GATTTCCTTC 
CCCCGCTCGG GAGTGGACAG CGATCGGGTT ACGCTGAAGG GTGCCAAGGA GTGCATTGAG 
GCGGCCAAGG CTCGCATCCT GGAGATCGTC GATGATTTGG AGCAGATGGT TACGATCGAG 
TGTATCATTC CGGCTCATAA CCATCGCATT GTTATGGGTA AGGGTGGTTC CAAGGTGCAA 
GGAATTACAT CGGAGTTTGA TGTGAACATT AAGTTCCCGG AACGAGACGC GCCGGAAGCG 
TTCGTTGAAG GGCAACAAAA TGGCGATGCA AATGGAGCGG AGCCCATTAA GACGACCGAT 
ATTATCAAGA TTAGTGGTCG CAAAGAGAAG TGCGAAAAGG CAAAGGAAGC TTTGTTGGCT 
TTGGTCCCGG TCACTGAGGA GATTAACGTT GCATTTGATC TGCACAGATC GTTGATTGGC 
CAGAAGGGTC GTGATGTCAA GGAGTTGATG AATACGTACG ATGTCCACAT TGAGCTGTCT 
CCACAGGATC AGAAGCTGGA CATCATCAAG GTCACTGGTG CCAGAGCGAA TATCGAAGAA 
GCCAAGATTG CAATTGCCAA GCGAATCGAA GAGCTCGAGG CTGATCGCAA GGATCGTGAA 
CTGCGTTCAT TTGAAGTCAA GGTCGAAATC GATCCGGAAT ACCATCCCAA GATCATCGGT 
CGTCGTGGTT TCGTCGTTAA TAAGATTCGT GCCACCCATG GAGTGCAGAT TTCGTTCCCG 
AAGCAAGATG ACCCTCAGAA CAACATCATC ACCATCCAAG GATACGAAAG TAACGCCCAT 
GCTGCCAAGG ATGAAATCTT AGGAATTGTC AACGAGCTTA ACTCGATGCA CCGCGAGGAA 
GTATCCATTG ATGAACGCGT CCACCGTCGT TTCATCGGCT TCAAGGGACG TCGTGTCAAG 
GAGATCAAGG AAATGTTCAA GGTGGAAATC AGCTTCCCAC GTCCGGAAGA TAAGGATCAG 
TCGCTTATCG TTATTGCTGG TAACAATCAG GACGACGTTG AAGCTTGTCG TGATCATCTG 
CTCAACTTGG AGGAAGAATT CCTCCAGGAC GTTGTTACCG CTGCACCACC GCAGCAAACC 
ACTTTCACCC AGTTCCTGGA GGACTCAATG AGCAATCGCG AGGCCACCAA GAAGGGCTTT 
GTCGTTCAAG GTGCCCCATG GGAACGCAAG ACTCCCAACA CTCAATCGCA GGAGGACTTC 
CCAGATTTCG GTCTTGGATC GGCTCCAGCT GAACAAGCTC CCCAGGCTCC AATCGCTTCG 
GCCTGGAATG CGCGCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021696448.1
CDS	240..3980
Translation	MEDSTVVDQQPSNSAYENNVASTPAPTPCYDDLFPALPESAPPRFNNPTLSAATQSMRVGSSVVTQVFIVPSGERKYDSDKFGEGESLRTCQNIMKETNAHIEISSGKDQSLTFLVTGKPNEVIEARRKILVHFQTQASKSISIPREHHRWILGKKGDRLRDLEKNTATKIVVPRINDESDIITISGTKEGIEKAEHEIRTTSDEQSRKAFERVNIPKIYHPFIMGPHNDNVSKMMEETGAKINIPPPSVQKDEIIITGEKEGVLQAKARIEAISKEMEKKCTSVGVEVPRAQHKYIVGPKGSTIQEILAMTGVSVEMPASDSPNDTITLRGPQDKLGNALSVVYQKANSVRTTTLECPQWIHKYIIGREGGHIKEFTAQYPNVHVEFNEDKIKIEGLPEQVDASSEALEKMVKDYTGRLTFTEMVVDPIYCKHIIGKNGANINRMKEEYEVLINIDEKDLKPIRIEGPADGVEKAKQELLEKIAKLENEKEESIVIDHRLFKTIIGAKGETIREIRDKHNQVQIIFPGATDKSDIVKIRGPRDDVDKCHKYLTKYVKELQKNSFVMEVPIFKQFHKYIIGKGGANIKKIRDETQTKIDLPAEGDKNEMIVITGKKDNVKEARNRILKIQEELSNIITEDIQIPPKYYNSLIGSGGKLISSIMEECGGVSIKFPSPESNSDKVAIRGPKEDVERAKQQLLELTNEKELSSFSAEVRAKPQHHKFLIGKNGASIKKIRDQTGARIVFPGYNDEDKEAIIIIGKKEGVEEAKAQLESIIKNIDNIVEDEISVEPRHHKHFVARRGEVLHRISEECGGVMISFPRSGVDSDRVTLKGAKECIEAAKARILEIVDDLEQMVTIECIIPAHNHRIVMGKGGSKVQGITSEFDVNIKFPERDAPEAFVEGQQNGDANGAEPIKTTDIIKISGRKEKCEKAKEALLALVPVTEEINVAFDLHRSLIGQKGRDVKELMNTYDVHIELSPQDQKLDIIKVTGARANIEEAKIAIAKRIEELEADRKDRELRSFEVKVEIDPEYHPKIIGRRGFVVNKIRATHGVQISFPKQDDPQNNIITIQGYESNAHAAKDEILGIVNELNSMHREEVSIDERVHRRFIGFKGRRVKEIKEMFKVEISFPRPEDKDQSLIVIAGNNQDDVEACRDHLLNLEEEFLQDVVTAAPPQQTTFTQFLEDSMSNREATKKGFVVQGAPWERKTPNTQSQEDFPDFGLGSAPAEQAPQAPIASAWNARH

Target ORF information:

RefSeq Version	XM_021840756.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti vigilin (LOC5570534), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021840756.1

ORF Insert Sequence:

ATGGAGGATT CAACAGTAGT TGACCAGCAG CCCAGCAACT CCGCGTACGA GAACAACGTT 
GCCTCGACGC CGGCTCCGAC CCCGTGCTAC GATGATCTGT TCCCGGCCCT GCCGGAGAGC 
GCCCCACCGC GCTTCAACAA TCCGACCCTG TCGGCGGCCA CGCAGAGTAT GCGCGTCGGC 
AGCTCGGTCG TGACGCAGGT CTTCATCGTG CCGTCCGGCG AGCGCAAGTA CGACTCGGAC 
AAGTTTGGCG AGGGCGAATC GTTGCGCACC TGCCAGAACA TCATGAAGGA AACCAACGCC 
CATATCGAGA TCTCGAGTGG CAAGGACCAA TCGTTGACCT TCCTGGTGAC TGGCAAGCCG 
AACGAGGTGA TCGAGGCCCG CCGTAAGATT TTGGTGCACT TCCAAACTCA GGCGAGCAAG 
TCGATCAGCA TTCCGCGGGA GCACCACCGC TGGATCCTCG GCAAGAAGGG CGACCGGCTG 
CGCGATCTGG AGAAGAACAC CGCCACCAAG ATCGTTGTGC CGCGCATCAA CGACGAGTCG 
GACATCATCA CCATCTCCGG TACCAAGGAA GGCATCGAGA AGGCCGAACA TGAAATCCGT 
ACCACGTCTG ATGAGCAGTC GCGTAAGGCC TTCGAGCGGG TTAACATCCC CAAGATTTAC 
CATCCGTTCA TCATGGGACC CCACAACGAC AACGTCAGCA AGATGATGGA GGAAACCGGA 
GCGAAGATCA ACATTCCGCC GCCGTCCGTC CAAAAGGATG AGATCATCAT CACCGGCGAG 
AAGGAAGGTG TACTCCAGGC AAAGGCACGC ATCGAAGCCA TCAGTAAGGA GATGGAAAAG 
AAGTGTACCA GCGTCGGCGT TGAGGTTCCC CGTGCTCAGC ATAAGTACAT CGTTGGTCCC 
AAGGGTTCCA CCATCCAGGA AATCTTGGCG ATGACCGGTG TATCGGTTGA GATGCCGGCT 
AGTGACTCTC CCAACGATAC CATCACTCTG CGCGGCCCCC AGGACAAGCT CGGAAATGCT 
TTGAGCGTTG TGTACCAGAA GGCGAACTCT GTTCGCACGA CTACGCTCGA GTGTCCCCAG 
TGGATTCATA AGTATATCAT TGGACGCGAG GGTGGCCATA TCAAGGAATT CACCGCTCAG 
TATCCCAACG TACACGTGGA GTTCAACGAA GACAAGATCA AGATCGAAGG CCTACCGGAA 
CAGGTGGATG CTTCTTCGGA AGCGCTGGAG AAAATGGTCA AGGATTACAC TGGACGTCTA 
ACCTTCACCG AAATGGTGGT TGATCCGATC TACTGCAAGC ACATCATCGG TAAGAACGGA 
GCCAACATCA ACCGTATGAA GGAGGAATAC GAGGTCTTGA TCAACATCGA CGAGAAGGAC 
TTGAAACCGA TCCGCATCGA AGGACCGGCC GACGGTGTTG AGAAGGCCAA GCAGGAGCTG 
CTTGAAAAGA TAGCGAAACT GGAAAACGAA AAGGAAGAGT CGATCGTGAT CGATCATCGT 
CTGTTCAAGA CGATCATCGG AGCGAAAGGC GAAACGATTC GGGAAATCCG TGACAAGCAT 
AACCAGGTGC AGATTATCTT CCCCGGAGCT ACCGACAAGT CGGACATTGT CAAGATCCGT 
GGTCCGCGTG ACGATGTGGA TAAGTGTCAC AAGTATCTGA CGAAGTACGT TAAGGAGCTG 
CAGAAGAACT CCTTCGTAAT GGAAGTCCCA ATCTTCAAGC AATTCCACAA GTACATTATC 
GGCAAGGGTG GTGCCAACAT CAAGAAGATT CGTGATGAGA CTCAAACGAA AATCGATCTT 
CCAGCAGAGG GTGACAAGAA CGAGATGATT GTCATCACTG GCAAGAAGGA CAACGTTAAG 
GAGGCTCGCA ATCGCATTCT GAAGATCCAG GAGGAACTTT CCAACATCAT CACCGAGGAT 
ATTCAGATCC CACCCAAGTA CTACAATTCG TTGATCGGAT CTGGTGGCAA GCTGATTTCC 
TCGATTATGG AAGAGTGCGG TGGGGTTTCG ATCAAGTTCC CAAGTCCGGA GTCCAACAGC 
GACAAGGTGG CCATCCGTGG CCCGAAGGAA GATGTCGAAC GAGCTAAGCA ACAGCTGCTC 
GAGCTGACCA ATGAGAAGGA GCTCTCTTCC TTCTCTGCCG AGGTTCGTGC CAAACCGCAG 
CACCATAAGT TCCTCATCGG TAAGAACGGC GCTTCCATCA AGAAGATCCG GGACCAAACC 
GGTGCTCGTA TTGTCTTCCC GGGTTACAAC GACGAGGACA AGGAGGCCAT CATCATCATC 
GGCAAGAAGG AAGGTGTCGA AGAGGCCAAG GCCCAGTTGG AGTCGATTAT CAAGAACATC 
GATAACATTG TTGAAGATGA GATTAGCGTG GAGCCGAGGC ACCATAAGCA TTTCGTGGCT 
CGCCGTGGCG AGGTGCTGCA CCGTATTTCG GAGGAGTGCG GTGGCGTGAT GATTTCCTTC 
CCCCGCTCGG GAGTGGACAG CGATCGGGTT ACGCTGAAGG GTGCCAAGGA GTGCATTGAG 
GCGGCCAAGG CTCGCATCCT GGAGATCGTC GATGATTTGG AGCAGATGGT TACGATCGAG 
TGTATCATTC CGGCTCATAA CCATCGCATT GTTATGGGTA AGGGTGGTTC CAAGGTGCAA 
GGAATTACAT CGGAGTTTGA TGTGAACATT AAGTTCCCGG AACGAGACGC GCCGGAAGCG 
TTCGTTGAAG GGCAACAAAA TGGCGATGCA AATGGAGCGG AGCCCATTAA GACGACCGAT 
ATTATCAAGA TTAGTGGTCG CAAAGAGAAG TGCGAAAAGG CAAAGGAAGC TTTGTTGGCT 
TTGGTCCCGG TCACTGAGGA GATTAACGTT GCATTTGATC TGCACAGATC GTTGATTGGC 
CAGAAGGGTC GTGATGTCAA GGAGTTGATG AATACGTACG ATGTCCACAT TGAGCTGTCT 
CCACAGGATC AGAAGCTGGA CATCATCAAG GTCACTGGTG CCAGAGCGAA TATCGAAGAA 
GCCAAGATTG CAATTGCCAA GCGAATCGAA GAGCTCGAGG CTGATCGCAA GGATCGTGAA 
CTGCGTTCAT TTGAAGTCAA GGTCGAAATC GATCCGGAAT ACCATCCCAA GATCATCGGT 
CGTCGTGGTT TCGTCGTTAA TAAGATTCGT GCCACCCATG GAGTGCAGAT TTCGTTCCCG 
AAGCAAGATG ACCCTCAGAA CAACATCATC ACCATCCAAG GATACGAAAG TAACGCCCAT 
GCTGCCAAGG ATGAAATCTT AGGAATTGTC AACGAGCTTA ACTCGATGCA CCGCGAGGAA 
GTATCCATTG ATGAACGCGT CCACCGTCGT TTCATCGGCT TCAAGGGACG TCGTGTCAAG 
GAGATCAAGG AAATGTTCAA GGTGGAAATC AGCTTCCCAC GTCCGGAAGA TAAGGATCAG 
TCGCTTATCG TTATTGCTGG TAACAATCAG GACGACGTTG AAGCTTGTCG TGATCATCTG 
CTCAACTTGG AGGAAGAATT CCTCCAGGAC GTTGTTACCG CTGCACCACC GCAGCAAACC 
ACTTTCACCC AGTTCCTGGA GGACTCAATG AGCAATCGCG AGGCCACCAA GAAGGGCTTT 
GTCGTTCAAG GTGCCCCATG GGAACGCAAG ACTCCCAACA CTCAATCGCA GGAGGACTTC 
CCAGATTTCG GTCTTGGATC GGCTCCAGCT GAACAAGCTC CCCAGGCTCC AATCGCTTCG 
GCCTGGAATG CGCGCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5570534 ( XM_021840757.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021696449.1 Aedes aegypti vigilin (LOC5570534), transcript variant X3, mRNA.

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CloneID	OAe119094
Clone ID Related Accession (Same CDS sequence)	XM_001659135.2 , XM_021840756.1 , XM_021840757.1
Accession Version	XM_021840757.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	vigilin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATT CAACAGTAGT TGACCAGCAG CCCAGCAACT CCGCGTACGA GAACAACGTT 
GCCTCGACGC CGGCTCCGAC CCCGTGCTAC GATGATCTGT TCCCGGCCCT GCCGGAGAGC 
GCCCCACCGC GCTTCAACAA TCCGACCCTG TCGGCGGCCA CGCAGAGTAT GCGCGTCGGC 
AGCTCGGTCG TGACGCAGGT CTTCATCGTG CCGTCCGGCG AGCGCAAGTA CGACTCGGAC 
AAGTTTGGCG AGGGCGAATC GTTGCGCACC TGCCAGAACA TCATGAAGGA AACCAACGCC 
CATATCGAGA TCTCGAGTGG CAAGGACCAA TCGTTGACCT TCCTGGTGAC TGGCAAGCCG 
AACGAGGTGA TCGAGGCCCG CCGTAAGATT TTGGTGCACT TCCAAACTCA GGCGAGCAAG 
TCGATCAGCA TTCCGCGGGA GCACCACCGC TGGATCCTCG GCAAGAAGGG CGACCGGCTG 
CGCGATCTGG AGAAGAACAC CGCCACCAAG ATCGTTGTGC CGCGCATCAA CGACGAGTCG 
GACATCATCA CCATCTCCGG TACCAAGGAA GGCATCGAGA AGGCCGAACA TGAAATCCGT 
ACCACGTCTG ATGAGCAGTC GCGTAAGGCC TTCGAGCGGG TTAACATCCC CAAGATTTAC 
CATCCGTTCA TCATGGGACC CCACAACGAC AACGTCAGCA AGATGATGGA GGAAACCGGA 
GCGAAGATCA ACATTCCGCC GCCGTCCGTC CAAAAGGATG AGATCATCAT CACCGGCGAG 
AAGGAAGGTG TACTCCAGGC AAAGGCACGC ATCGAAGCCA TCAGTAAGGA GATGGAAAAG 
AAGTGTACCA GCGTCGGCGT TGAGGTTCCC CGTGCTCAGC ATAAGTACAT CGTTGGTCCC 
AAGGGTTCCA CCATCCAGGA AATCTTGGCG ATGACCGGTG TATCGGTTGA GATGCCGGCT 
AGTGACTCTC CCAACGATAC CATCACTCTG CGCGGCCCCC AGGACAAGCT CGGAAATGCT 
TTGAGCGTTG TGTACCAGAA GGCGAACTCT GTTCGCACGA CTACGCTCGA GTGTCCCCAG 
TGGATTCATA AGTATATCAT TGGACGCGAG GGTGGCCATA TCAAGGAATT CACCGCTCAG 
TATCCCAACG TACACGTGGA GTTCAACGAA GACAAGATCA AGATCGAAGG CCTACCGGAA 
CAGGTGGATG CTTCTTCGGA AGCGCTGGAG AAAATGGTCA AGGATTACAC TGGACGTCTA 
ACCTTCACCG AAATGGTGGT TGATCCGATC TACTGCAAGC ACATCATCGG TAAGAACGGA 
GCCAACATCA ACCGTATGAA GGAGGAATAC GAGGTCTTGA TCAACATCGA CGAGAAGGAC 
TTGAAACCGA TCCGCATCGA AGGACCGGCC GACGGTGTTG AGAAGGCCAA GCAGGAGCTG 
CTTGAAAAGA TAGCGAAACT GGAAAACGAA AAGGAAGAGT CGATCGTGAT CGATCATCGT 
CTGTTCAAGA CGATCATCGG AGCGAAAGGC GAAACGATTC GGGAAATCCG TGACAAGCAT 
AACCAGGTGC AGATTATCTT CCCCGGAGCT ACCGACAAGT CGGACATTGT CAAGATCCGT 
GGTCCGCGTG ACGATGTGGA TAAGTGTCAC AAGTATCTGA CGAAGTACGT TAAGGAGCTG 
CAGAAGAACT CCTTCGTAAT GGAAGTCCCA ATCTTCAAGC AATTCCACAA GTACATTATC 
GGCAAGGGTG GTGCCAACAT CAAGAAGATT CGTGATGAGA CTCAAACGAA AATCGATCTT 
CCAGCAGAGG GTGACAAGAA CGAGATGATT GTCATCACTG GCAAGAAGGA CAACGTTAAG 
GAGGCTCGCA ATCGCATTCT GAAGATCCAG GAGGAACTTT CCAACATCAT CACCGAGGAT 
ATTCAGATCC CACCCAAGTA CTACAATTCG TTGATCGGAT CTGGTGGCAA GCTGATTTCC 
TCGATTATGG AAGAGTGCGG TGGGGTTTCG ATCAAGTTCC CAAGTCCGGA GTCCAACAGC 
GACAAGGTGG CCATCCGTGG CCCGAAGGAA GATGTCGAAC GAGCTAAGCA ACAGCTGCTC 
GAGCTGACCA ATGAGAAGGA GCTCTCTTCC TTCTCTGCCG AGGTTCGTGC CAAACCGCAG 
CACCATAAGT TCCTCATCGG TAAGAACGGC GCTTCCATCA AGAAGATCCG GGACCAAACC 
GGTGCTCGTA TTGTCTTCCC GGGTTACAAC GACGAGGACA AGGAGGCCAT CATCATCATC 
GGCAAGAAGG AAGGTGTCGA AGAGGCCAAG GCCCAGTTGG AGTCGATTAT CAAGAACATC 
GATAACATTG TTGAAGATGA GATTAGCGTG GAGCCGAGGC ACCATAAGCA TTTCGTGGCT 
CGCCGTGGCG AGGTGCTGCA CCGTATTTCG GAGGAGTGCG GTGGCGTGAT GATTTCCTTC 
CCCCGCTCGG GAGTGGACAG CGATCGGGTT ACGCTGAAGG GTGCCAAGGA GTGCATTGAG 
GCGGCCAAGG CTCGCATCCT GGAGATCGTC GATGATTTGG AGCAGATGGT TACGATCGAG 
TGTATCATTC CGGCTCATAA CCATCGCATT GTTATGGGTA AGGGTGGTTC CAAGGTGCAA 
GGAATTACAT CGGAGTTTGA TGTGAACATT AAGTTCCCGG AACGAGACGC GCCGGAAGCG 
TTCGTTGAAG GGCAACAAAA TGGCGATGCA AATGGAGCGG AGCCCATTAA GACGACCGAT 
ATTATCAAGA TTAGTGGTCG CAAAGAGAAG TGCGAAAAGG CAAAGGAAGC TTTGTTGGCT 
TTGGTCCCGG TCACTGAGGA GATTAACGTT GCATTTGATC TGCACAGATC GTTGATTGGC 
CAGAAGGGTC GTGATGTCAA GGAGTTGATG AATACGTACG ATGTCCACAT TGAGCTGTCT 
CCACAGGATC AGAAGCTGGA CATCATCAAG GTCACTGGTG CCAGAGCGAA TATCGAAGAA 
GCCAAGATTG CAATTGCCAA GCGAATCGAA GAGCTCGAGG CTGATCGCAA GGATCGTGAA 
CTGCGTTCAT TTGAAGTCAA GGTCGAAATC GATCCGGAAT ACCATCCCAA GATCATCGGT 
CGTCGTGGTT TCGTCGTTAA TAAGATTCGT GCCACCCATG GAGTGCAGAT TTCGTTCCCG 
AAGCAAGATG ACCCTCAGAA CAACATCATC ACCATCCAAG GATACGAAAG TAACGCCCAT 
GCTGCCAAGG ATGAAATCTT AGGAATTGTC AACGAGCTTA ACTCGATGCA CCGCGAGGAA 
GTATCCATTG ATGAACGCGT CCACCGTCGT TTCATCGGCT TCAAGGGACG TCGTGTCAAG 
GAGATCAAGG AAATGTTCAA GGTGGAAATC AGCTTCCCAC GTCCGGAAGA TAAGGATCAG 
TCGCTTATCG TTATTGCTGG TAACAATCAG GACGACGTTG AAGCTTGTCG TGATCATCTG 
CTCAACTTGG AGGAAGAATT CCTCCAGGAC GTTGTTACCG CTGCACCACC GCAGCAAACC 
ACTTTCACCC AGTTCCTGGA GGACTCAATG AGCAATCGCG AGGCCACCAA GAAGGGCTTT 
GTCGTTCAAG GTGCCCCATG GGAACGCAAG ACTCCCAACA CTCAATCGCA GGAGGACTTC 
CCAGATTTCG GTCTTGGATC GGCTCCAGCT GAACAAGCTC CCCAGGCTCC AATCGCTTCG 
GCCTGGAATG CGCGCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021696449.1
CDS	192..3932
Translation	MEDSTVVDQQPSNSAYENNVASTPAPTPCYDDLFPALPESAPPRFNNPTLSAATQSMRVGSSVVTQVFIVPSGERKYDSDKFGEGESLRTCQNIMKETNAHIEISSGKDQSLTFLVTGKPNEVIEARRKILVHFQTQASKSISIPREHHRWILGKKGDRLRDLEKNTATKIVVPRINDESDIITISGTKEGIEKAEHEIRTTSDEQSRKAFERVNIPKIYHPFIMGPHNDNVSKMMEETGAKINIPPPSVQKDEIIITGEKEGVLQAKARIEAISKEMEKKCTSVGVEVPRAQHKYIVGPKGSTIQEILAMTGVSVEMPASDSPNDTITLRGPQDKLGNALSVVYQKANSVRTTTLECPQWIHKYIIGREGGHIKEFTAQYPNVHVEFNEDKIKIEGLPEQVDASSEALEKMVKDYTGRLTFTEMVVDPIYCKHIIGKNGANINRMKEEYEVLINIDEKDLKPIRIEGPADGVEKAKQELLEKIAKLENEKEESIVIDHRLFKTIIGAKGETIREIRDKHNQVQIIFPGATDKSDIVKIRGPRDDVDKCHKYLTKYVKELQKNSFVMEVPIFKQFHKYIIGKGGANIKKIRDETQTKIDLPAEGDKNEMIVITGKKDNVKEARNRILKIQEELSNIITEDIQIPPKYYNSLIGSGGKLISSIMEECGGVSIKFPSPESNSDKVAIRGPKEDVERAKQQLLELTNEKELSSFSAEVRAKPQHHKFLIGKNGASIKKIRDQTGARIVFPGYNDEDKEAIIIIGKKEGVEEAKAQLESIIKNIDNIVEDEISVEPRHHKHFVARRGEVLHRISEECGGVMISFPRSGVDSDRVTLKGAKECIEAAKARILEIVDDLEQMVTIECIIPAHNHRIVMGKGGSKVQGITSEFDVNIKFPERDAPEAFVEGQQNGDANGAEPIKTTDIIKISGRKEKCEKAKEALLALVPVTEEINVAFDLHRSLIGQKGRDVKELMNTYDVHIELSPQDQKLDIIKVTGARANIEEAKIAIAKRIEELEADRKDRELRSFEVKVEIDPEYHPKIIGRRGFVVNKIRATHGVQISFPKQDDPQNNIITIQGYESNAHAAKDEILGIVNELNSMHREEVSIDERVHRRFIGFKGRRVKEIKEMFKVEISFPRPEDKDQSLIVIAGNNQDDVEACRDHLLNLEEEFLQDVVTAAPPQQTTFTQFLEDSMSNREATKKGFVVQGAPWERKTPNTQSQEDFPDFGLGSAPAEQAPQAPIASAWNARH

Target ORF information:

RefSeq Version	XM_021840757.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti vigilin (LOC5570534), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021840757.1

ORF Insert Sequence:

ATGGAGGATT CAACAGTAGT TGACCAGCAG CCCAGCAACT CCGCGTACGA GAACAACGTT 
GCCTCGACGC CGGCTCCGAC CCCGTGCTAC GATGATCTGT TCCCGGCCCT GCCGGAGAGC 
GCCCCACCGC GCTTCAACAA TCCGACCCTG TCGGCGGCCA CGCAGAGTAT GCGCGTCGGC 
AGCTCGGTCG TGACGCAGGT CTTCATCGTG CCGTCCGGCG AGCGCAAGTA CGACTCGGAC 
AAGTTTGGCG AGGGCGAATC GTTGCGCACC TGCCAGAACA TCATGAAGGA AACCAACGCC 
CATATCGAGA TCTCGAGTGG CAAGGACCAA TCGTTGACCT TCCTGGTGAC TGGCAAGCCG 
AACGAGGTGA TCGAGGCCCG CCGTAAGATT TTGGTGCACT TCCAAACTCA GGCGAGCAAG 
TCGATCAGCA TTCCGCGGGA GCACCACCGC TGGATCCTCG GCAAGAAGGG CGACCGGCTG 
CGCGATCTGG AGAAGAACAC CGCCACCAAG ATCGTTGTGC CGCGCATCAA CGACGAGTCG 
GACATCATCA CCATCTCCGG TACCAAGGAA GGCATCGAGA AGGCCGAACA TGAAATCCGT 
ACCACGTCTG ATGAGCAGTC GCGTAAGGCC TTCGAGCGGG TTAACATCCC CAAGATTTAC 
CATCCGTTCA TCATGGGACC CCACAACGAC AACGTCAGCA AGATGATGGA GGAAACCGGA 
GCGAAGATCA ACATTCCGCC GCCGTCCGTC CAAAAGGATG AGATCATCAT CACCGGCGAG 
AAGGAAGGTG TACTCCAGGC AAAGGCACGC ATCGAAGCCA TCAGTAAGGA GATGGAAAAG 
AAGTGTACCA GCGTCGGCGT TGAGGTTCCC CGTGCTCAGC ATAAGTACAT CGTTGGTCCC 
AAGGGTTCCA CCATCCAGGA AATCTTGGCG ATGACCGGTG TATCGGTTGA GATGCCGGCT 
AGTGACTCTC CCAACGATAC CATCACTCTG CGCGGCCCCC AGGACAAGCT CGGAAATGCT 
TTGAGCGTTG TGTACCAGAA GGCGAACTCT GTTCGCACGA CTACGCTCGA GTGTCCCCAG 
TGGATTCATA AGTATATCAT TGGACGCGAG GGTGGCCATA TCAAGGAATT CACCGCTCAG 
TATCCCAACG TACACGTGGA GTTCAACGAA GACAAGATCA AGATCGAAGG CCTACCGGAA 
CAGGTGGATG CTTCTTCGGA AGCGCTGGAG AAAATGGTCA AGGATTACAC TGGACGTCTA 
ACCTTCACCG AAATGGTGGT TGATCCGATC TACTGCAAGC ACATCATCGG TAAGAACGGA 
GCCAACATCA ACCGTATGAA GGAGGAATAC GAGGTCTTGA TCAACATCGA CGAGAAGGAC 
TTGAAACCGA TCCGCATCGA AGGACCGGCC GACGGTGTTG AGAAGGCCAA GCAGGAGCTG 
CTTGAAAAGA TAGCGAAACT GGAAAACGAA AAGGAAGAGT CGATCGTGAT CGATCATCGT 
CTGTTCAAGA CGATCATCGG AGCGAAAGGC GAAACGATTC GGGAAATCCG TGACAAGCAT 
AACCAGGTGC AGATTATCTT CCCCGGAGCT ACCGACAAGT CGGACATTGT CAAGATCCGT 
GGTCCGCGTG ACGATGTGGA TAAGTGTCAC AAGTATCTGA CGAAGTACGT TAAGGAGCTG 
CAGAAGAACT CCTTCGTAAT GGAAGTCCCA ATCTTCAAGC AATTCCACAA GTACATTATC 
GGCAAGGGTG GTGCCAACAT CAAGAAGATT CGTGATGAGA CTCAAACGAA AATCGATCTT 
CCAGCAGAGG GTGACAAGAA CGAGATGATT GTCATCACTG GCAAGAAGGA CAACGTTAAG 
GAGGCTCGCA ATCGCATTCT GAAGATCCAG GAGGAACTTT CCAACATCAT CACCGAGGAT 
ATTCAGATCC CACCCAAGTA CTACAATTCG TTGATCGGAT CTGGTGGCAA GCTGATTTCC 
TCGATTATGG AAGAGTGCGG TGGGGTTTCG ATCAAGTTCC CAAGTCCGGA GTCCAACAGC 
GACAAGGTGG CCATCCGTGG CCCGAAGGAA GATGTCGAAC GAGCTAAGCA ACAGCTGCTC 
GAGCTGACCA ATGAGAAGGA GCTCTCTTCC TTCTCTGCCG AGGTTCGTGC CAAACCGCAG 
CACCATAAGT TCCTCATCGG TAAGAACGGC GCTTCCATCA AGAAGATCCG GGACCAAACC 
GGTGCTCGTA TTGTCTTCCC GGGTTACAAC GACGAGGACA AGGAGGCCAT CATCATCATC 
GGCAAGAAGG AAGGTGTCGA AGAGGCCAAG GCCCAGTTGG AGTCGATTAT CAAGAACATC 
GATAACATTG TTGAAGATGA GATTAGCGTG GAGCCGAGGC ACCATAAGCA TTTCGTGGCT 
CGCCGTGGCG AGGTGCTGCA CCGTATTTCG GAGGAGTGCG GTGGCGTGAT GATTTCCTTC 
CCCCGCTCGG GAGTGGACAG CGATCGGGTT ACGCTGAAGG GTGCCAAGGA GTGCATTGAG 
GCGGCCAAGG CTCGCATCCT GGAGATCGTC GATGATTTGG AGCAGATGGT TACGATCGAG 
TGTATCATTC CGGCTCATAA CCATCGCATT GTTATGGGTA AGGGTGGTTC CAAGGTGCAA 
GGAATTACAT CGGAGTTTGA TGTGAACATT AAGTTCCCGG AACGAGACGC GCCGGAAGCG 
TTCGTTGAAG GGCAACAAAA TGGCGATGCA AATGGAGCGG AGCCCATTAA GACGACCGAT 
ATTATCAAGA TTAGTGGTCG CAAAGAGAAG TGCGAAAAGG CAAAGGAAGC TTTGTTGGCT 
TTGGTCCCGG TCACTGAGGA GATTAACGTT GCATTTGATC TGCACAGATC GTTGATTGGC 
CAGAAGGGTC GTGATGTCAA GGAGTTGATG AATACGTACG ATGTCCACAT TGAGCTGTCT 
CCACAGGATC AGAAGCTGGA CATCATCAAG GTCACTGGTG CCAGAGCGAA TATCGAAGAA 
GCCAAGATTG CAATTGCCAA GCGAATCGAA GAGCTCGAGG CTGATCGCAA GGATCGTGAA 
CTGCGTTCAT TTGAAGTCAA GGTCGAAATC GATCCGGAAT ACCATCCCAA GATCATCGGT 
CGTCGTGGTT TCGTCGTTAA TAAGATTCGT GCCACCCATG GAGTGCAGAT TTCGTTCCCG 
AAGCAAGATG ACCCTCAGAA CAACATCATC ACCATCCAAG GATACGAAAG TAACGCCCAT 
GCTGCCAAGG ATGAAATCTT AGGAATTGTC AACGAGCTTA ACTCGATGCA CCGCGAGGAA 
GTATCCATTG ATGAACGCGT CCACCGTCGT TTCATCGGCT TCAAGGGACG TCGTGTCAAG 
GAGATCAAGG AAATGTTCAA GGTGGAAATC AGCTTCCCAC GTCCGGAAGA TAAGGATCAG 
TCGCTTATCG TTATTGCTGG TAACAATCAG GACGACGTTG AAGCTTGTCG TGATCATCTG 
CTCAACTTGG AGGAAGAATT CCTCCAGGAC GTTGTTACCG CTGCACCACC GCAGCAAACC 
ACTTTCACCC AGTTCCTGGA GGACTCAATG AGCAATCGCG AGGCCACCAA GAAGGGCTTT 
GTCGTTCAAG GTGCCCCATG GGAACGCAAG ACTCCCAACA CTCAATCGCA GGAGGACTTC 
CCAGATTTCG GTCTTGGATC GGCTCCAGCT GAACAAGCTC CCCAGGCTCC AATCGCTTCG 
GCCTGGAATG CGCGCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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LOC5570534 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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