AAEL_AAEL005234 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	AAEL_AAEL005234
Entrez Gene ID	5566154
Full Name	titin
Synonyms	AAEL005234
Gene Type	protein-coding
Organism	Aedes aegypti(Yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021849577.1	XP_021705269.1	titin
XM_001650487.1	XP_001650537.1	AAEL005234-PA

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AAEL_AAEL005234 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AAEL_AAEL005234 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAe02282	XM_001650487.1 Latest version!	Aedes aegypti AAEL005234-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OAe125614	XM_021849577.1 Latest version!	Aedes aegypti titin (LOC5566154), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AAEL_AAEL005234 ( XM_001650487.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_001650537.1 Aedes aegypti AAEL005234-RA partial mRNA.

Price & Availability↑

CloneID	OAe02282
Clone ID Related Accession (Same CDS sequence)	XM_001650487.1
Accession Version	XM_001650487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL005234-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001810307). COMPLETENESS: incomplete on the 5' end.

ATGGAGCAAA GTTTGGCTGA CGAAATAGGG GTTCAAGGAG CGAAAAGGTC ACTATGCTTG 
CGATGGGTTT CACCACGGCT GCATAATGCG GAAACGAATA CTCAGCCGGT CCCTACCATC 
GTTACTCAAG AACCAAAGGA TATCCCCCTA GCAAAGCGAA CCAAGCCACC GGTGGCCATC 
ATTCGATACA GTCCCCAATT AGAAGAACCC ATCGCAGCAC ATGAGCTGTC CACCATTCCC 
GAAGTCGAAA CTCCGCTTGC CTCCAAGATC AACATCTCGG TAGTACCTAG CGAAAAGCTC 
GATCCTGCCG AACAACTGCT CATTGAAGTG CGCGACAAAC TTCTGGACCA CGCCCGTGCC 
CTTGGCTACA AAGATTTTCC CAACTACCAG CAATACGTCC GAGAGCAGAA TCTGGAAGGC 
ACCCGATACG ATCCGGAGAA GACCAGCTAC TCGCGCCTTC ACGAACTGCT GGAAGTCACC 
GACAAGAGTG ACCACCTTCG CTATCAAAAC TTTCCCGTAC CGGCCCCGGA GATGAACGTT 
ACTGAAGAGA GTGCGAATGC GAACAACAAA AAGCCCGAAT TGGAAAGCAA CTCCAGTTCC 
AGCACCACAC TCCCGGACGT GGTGGCCGAG CTCAAGCTCA GAAAGATTAT CGATAAATCT 
TTCGACAACT CTCTTGACGA TTCCAACCTT TCGACACCGG ATTCTGGTTC GTTACTAGCT 
CTTCATCCTC TTCCAGAAAA ATCCCCGATA AAATCGCACC AGAATCAAAA CGATCCCCGC 
AAAACCCCCG ACCAATCCGT CTCGGAGACA ACGGCATCCC TCGAACGGGA TCTCAATCAG 
CTTGGCCTGC GCTGGGCCTC AAGTATGCTG AAGAAAAACC AGCAAGCCCT CCTACAAGAG 
CACTCCAGCA GCTCGTCCCT CTCACCGGCC GAAGACATCC GCCGCCTGTC GGCCAGAAAA 
TCCAAACCAC TGCCATCTCT TCGGGACGAC CCAAACGATT CGCAGAACAC AGTCTCCGGT 
AAACCCTTGA ACCTTAAAGA GTTCGTTGCC CGTGAACTGA TGATACGGAC CCATTCCGAT 
CCGAACTCGC TCTCGGATTC GTCCAGTCCG TGCAGTATGC TGTTACGATC ATTGCTGGAC 
ATCAGCCACA TTAACACCTC CACTCCGGAA TTGCTCACGC AAACCACGGA TAAGAACATT 
CAGCGAACGT CCACTCCGGT GGCCACGAAA TCGTCCTCCG GCGGGACCCC ACGGGAAATC 
GCTGTCGCCA ATGATGGCAG CCTGAATGTT ACGAATGGGC TATTCTCCGG CGAATCGCGA 
ATTTCGTCCG TTCACATGAG TTCTTCTTCG GGCGGGGAGA ACAAACTGAC CGTTCCGAAC 
GTGCGCCTCG ATGTGCACCG GTATGCAAAG GGACGGGACA CAGCCCAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001650537.1
CDS	1..1434
Translation	MEQSLADEIGVQGAKRSLCLRWVSPRLHNAETNTQPVPTIVTQEPKDIPLAKRTKPPVAIIRYSPQLEEPIAAHELSTIPEVETPLASKINISVVPSEKLDPAEQLLIEVRDKLLDHARALGYKDFPNYQQYVREQNLEGTRYDPEKTSYSRLHELLEVTDKSDHLRYQNFPVPAPEMNVTEESANANNKKPELESNSSSSTTLPDVVAELKLRKIIDKSFDNSLDDSNLSTPDSGSLLALHPLPEKSPIKSHQNQNDPRKTPDQSVSETTASLERDLNQLGLRWASSMLKKNQQALLQEHSSSSSLSPAEDIRRLSARKSKPLPSLRDDPNDSQNTVSGKPLNLKEFVARELMIRTHSDPNSLSDSSSPCSMLLRSLLDISHINTSTPELLTQTTDKNIQRTSTPVATKSSSGGTPREIAVANDGSLNVTNGLFSGESRISSVHMSSSSGGENKLTVPNVRLDVHRYAKGRDTAQK

Target ORF information:

RefSeq Version	XM_001650487.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL005234-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001650487.1

ORF Insert Sequence:

ATGGAGCAAA GTTTGGCTGA CGAAATAGGG GTTCAAGGAG CGAAAAGGTC ACTATGCTTG 
CGATGGGTTT CACCACGGCT GCATAATGCG GAAACGAATA CTCAGCCGGT CCCTACCATC 
GTTACTCAAG AACCAAAGGA TATCCCCCTA GCAAAGCGAA CCAAGCCACC GGTGGCCATC 
ATTCGATACA GTCCCCAATT AGAAGAACCC ATCGCAGCAC ATGAGCTGTC CACCATTCCC 
GAAGTCGAAA CTCCGCTTGC CTCCAAGATC AACATCTCGG TAGTACCTAG CGAAAAGCTC 
GATCCTGCCG AACAACTGCT CATTGAAGTG CGCGACAAAC TTCTGGACCA CGCCCGTGCC 
CTTGGCTACA AAGATTTTCC CAACTACCAG CAATACGTCC GAGAGCAGAA TCTGGAAGGC 
ACCCGATACG ATCCGGAGAA GACCAGCTAC TCGCGCCTTC ACGAACTGCT GGAAGTCACC 
GACAAGAGTG ACCACCTTCG CTATCAAAAC TTTCCCGTAC CGGCCCCGGA GATGAACGTT 
ACTGAAGAGA GTGCGAATGC GAACAACAAA AAGCCCGAAT TGGAAAGCAA CTCCAGTTCC 
AGCACCACAC TCCCGGACGT GGTGGCCGAG CTCAAGCTCA GAAAGATTAT CGATAAATCT 
TTCGACAACT CTCTTGACGA TTCCAACCTT TCGACACCGG ATTCTGGTTC GTTACTAGCT 
CTTCATCCTC TTCCAGAAAA ATCCCCGATA AAATCGCACC AGAATCAAAA CGATCCCCGC 
AAAACCCCCG ACCAATCCGT CTCGGAGACA ACGGCATCCC TCGAACGGGA TCTCAATCAG 
CTTGGCCTGC GCTGGGCCTC AAGTATGCTG AAGAAAAACC AGCAAGCCCT CCTACAAGAG 
CACTCCAGCA GCTCGTCCCT CTCACCGGCC GAAGACATCC GCCGCCTGTC GGCCAGAAAA 
TCCAAACCAC TGCCATCTCT TCGGGACGAC CCAAACGATT CGCAGAACAC AGTCTCCGGT 
AAACCCTTGA ACCTTAAAGA GTTCGTTGCC CGTGAACTGA TGATACGGAC CCATTCCGAT 
CCGAACTCGC TCTCGGATTC GTCCAGTCCG TGCAGTATGC TGTTACGATC ATTGCTGGAC 
ATCAGCCACA TTAACACCTC CACTCCGGAA TTGCTCACGC AAACCACGGA TAAGAACATT 
CAGCGAACGT CCACTCCGGT GGCCACGAAA TCGTCCTCCG GCGGGACCCC ACGGGAAATC 
GCTGTCGCCA ATGATGGCAG CCTGAATGTT ACGAATGGGC TATTCTCCGG CGAATCGCGA 
ATTTCGTCCG TTCACATGAG TTCTTCTTCG GGCGGGGAGA ACAAACTGAC CGTTCCGAAC 
GTGCGCCTCG ATGTGCACCG GTATGCAAAG GGACGGGACA CAGCCCAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL005234 ( XM_021849577.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_021705269.1 Aedes aegypti titin (LOC5566154), mRNA.

Price & Availability↑

CloneID	OAe125614
Clone ID Related Accession (Same CDS sequence)	XM_021849577.1
Accession Version	XM_021849577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	titin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATCA ACATTACGGT GCACAGTTCG CAGGCTCCGT CTTCTTCCGG TGGGAATCCG 
TTTGCCAACT TGGAAAGCGT TCGTAAGGCC GAAAATGACA AACTGCGGAA AGCCCGATTG 
GAACAGGTCC GACAGCAGTC GAAGCAACTG GCGGCGAAAG TTCGTCGAAA CTTCCAAACT 
GCCAAGGAGA AAGAGTTGGC CAATGTGGAG AAACTGAGGG AAGCGGAATT GAAGAACTGG 
AAGCAGAGGC ACATAGCGAA GCTGCAGGCC GATTATCAAC ACTGTTTGGA GGATGTCGGT 
GAGGCGCATA AAGCTGCGGA AGCGGCTGAG CAGTGTGAAG TTTGGTTCCA GGAGAAAAAG 
GCCACCCAAC AGGCGGCAGC TCTTCAGAGG GGACGGCAAG CGGAGCAAAA ACTAGCGAAG 
GACATTCAGG ACCGAGAAGC TAGGAAGTCG AAGAAAAAGA AAGAGTATGT TCCGACCAAA 
TCGGTTGCGG TGCAAGTTTC GGCTCCCGCG GAGCAGAAAA AGGATGCCCC CGTGCAGGTG 
GATGATAGTA GTACCTACGC CAATACTGTG AGTGCATTTG AGCAGTTCAA GAAAGGTTCG 
AAGGCAAACT CGGTGCCGGA TCTACGCATG CATGACCGAC CGTTGTCACC GGATTTCCTG 
TCGTCCGATG AAGAGCTACT CCATGGTATT GATAAGAACA AAGAGAACAT TCCAACTATC 
AATTCGACAA CCAGCAAACG AATAGACACA CTTTACTGCA ATCCAACATC ATTTACCTCA 
CCAGAAACAA TTCAACAGCA AGCGTCCGCA ATCCGACAGC AACCGATAAA ACCTTTCACG 
CAGGTATCGG AGTTCGTCAA ACGACGCAGG GAAGAGAAGT TACACCAACA CGCACACCCA 
CACGAACCCA TCACGAAGGA CATCCCCACA TACTGCTCCC AAAAAAATGT GCAATTCGAT 
GACCTCAGTG AGAACACTTT ATCCTTTCCC ACGAGCACAG CCCTGGATCG ATCCATCTTG 
GAATCGCCAC GCAAACCGGT CCGAAAGCTG GTCGAACAGC CCAAGGTCAT TCCTCTCAAG 
GGCAAACCAA GTCCGTCGAA GCGATCGACG ACGGATTCCA ATTCGGCCGG AGCCACTTCC 
TCCAAGGTGC AGTACTACGA CTACAACACC AAGTACCGGA AGGAATACGA CCAACCGAGT 
GCGTTTGTGC AGCGCAATGA CAAACCAAGG GAAGGGGAGC TGAATGCCAT GCAGGAGGCC 
AGCCGACTGG AGCGGCTGCA GGCGGAGATG CTGAAAGCGA GAAGACAAAT TCCAGCGGGG 
CCGGATCGCT CGCAGACAGC GCTGGAGAAG CTACAGATGC GAAAGGACTA CGAGAACCTT 
CAGTTAGAAC TGGACAAATT GATCAAAGCT GAGTCTCAAG CCAAGGGCCA GGATGTGACA 
AGAAAACCAC TAATGACCGA GACCCGCCAG CGGCAGCAAA CGGAAGCCCG CCAGAAGCGA 
ATGAATCAAG CCGTGGAAGA TTTACTGAAG AAGCGAGTGC TGATCACTTG TCCCATAGTT 
CGGGATCCAC CCCGAGGAGT TCAGTTGTCA AATAGACCTT CACAGGTAAA CGTTGCGGCG 
GGGATTGGGA ATGCTGTCCA TGTGCCCGGA GATGATTCCA TCCTGCGGGA AGCAACAAAC 
AGCTCAGACA GTTGCAGCAC TATCATGCTG GGTTACGATC AAAGTAACGT GCGCGCGGGA 
ATTCCCCTAC CGGAAGCTGA TAAAGTCGCT AAGCTGAAGG AACTGCTGCA GAAGGTGAAC 
GAACAGAAAC GACTACTAAC GGAGGAGCTT AAGAAAGAGG AGGAAATCGA ACATGAACTG 
AAGGTGCCTA TTGTGAATAT CAAATCGACG AAAACCCAAA CGGATAACGG AATCAAGAAG 
TTGCAACAAC GACAGCAGGA ACTTGAAGCC CAGCAAAAGC AACTGCACGA GAAGGAAAAG 
GAAATTCGTG AGTTGGAGAA GCAGTTGAAG GAGAAGTTGA ATCGGTTGGA GAAAGAAAAG 
TCCAAGTCAA AGGTTCCGAT TCACATAGAA ACGAACGGTA CCGGAAGTGC AGATGTCCAA 
ATGGCTCATG ATAACAGCTC AACTGGATCG AATGATAGCA TTCAATCGGG TTCAGAAATA 
CCGGTAAAAA TTGTTATAAC TGTGAATGAT AAATCTAAAA AGAAAGTCAA GAAGACACCG 
AAGAAGATAC TCCCGGAAGC TAGGAAAAGG AAGAAAGCTA AGGATGTCGT TGATCGACCT 
ATAGAATCAC AGAAAACTAG AGGCAAGTCT CCAAAGAAGG TGGAAGCAAT CCCCGAAAAG 
CCCATTGAAG TTCCAAAAGT TCACTCAGAA CCACCGAGAA CAGTTCCAGA ACCGATGGAA 
GCGTCACCAA TCAGCTCAAC GACCTCAACC GTTTATCGAC AACTGCCATC AAAAATCGAC 
AATCGCGCCG GCCAGCTCTT GAAGCAATTG GCCCAACAAC AAGCACCGCC ACCGAAGCCT 
GTAAAATCCC AACGCCGGGA ATCTGCGCCC AAGCATCAAC TAAAAAAGAC CACCCCTCCT 
CCTGCTCGAC CAGCACCAAT TGCTCAAGCC CAGCAGCGAG GGCAAAATTT CAATCCCAAC 
CTCATGCAGT ACATCGTTCG ATTGTTGGGT ATGTCCCGGC ATTCAATCGA GCAACTCGGG 
GTCAGTTCAT CAACGACGGT TTCCACGCCG CACGATTCCG TTACCAACGT CAGCGCCAAT 
CAATCCAGCA GCGTCGAAGA GCAAACCAGT CGCGATCAGG TGCGAATGGA CAAGCTGAGA 
CGATTCATCG ACGAAAACTA TAACTTCCTC CAGGAAATCG ACGAAACCCT CAAAGAGCAG 
GAGCTGAGCG GAACGGCCGA TGATAACATC AGTCGAGTGG AGGACGTTTG GATGAAAACG 
CTCAGTCGGA AGGAGAAGGA AGTTCGACGA GAAAAACCGC AAATTGCGGC ACCTCCCCTC 
GGCCAATCAC CCCCAGTCAG GGACAGTTCT CTCAAGTCGA TTCTTAAGTC ACCTAAAAAG 
ACACCCGCCA AGGTGGCGAC AATCATGACC CCGCAAGGAA ACGTGGAAGT AATCGACCTA 
ACAGACCGTG CTGAACAAGA AGTGCTGGAA AAGTACTCGC ATCTGGCGGA AAACTGTAGC 
AAGCGAATCT CCGAATTGTC GGACATGATC CAGAAGGTCC GGGAGGAGAA GAAAAAGCTC 
ATCGAAAACA GTTTGTCATC CAGCGAGCAG CCGGAATCGT CCACCAAATA TCTGGATTTG 
CCACGATCGC AACAGAAAGC GCCCGCTGCC GAAACGAGTC CGCAGTCGGC CAAGGAGGAT 
CCCGTCAGCG AAGAGATCAA CAACATTTTC ACCAAGTCGA GACAGATTGG ACTCAGCAAG 
GATAGTGGCA TAGCAATGTC ACGACCGGTG ACATCATCAG ACTATCGTGA CAGCCCGGAC 
GCAAGACCGC CCAACGCCGG GGCACCACAG TTCGAGTCGT CCGAGGAGCT GAGCTCACGC 
GAGATGGCAC CCACTGGTTC GGTGGCGTTT GAACCCTTGC TCAAGGATAT TCCGAAGGTT 
TCACCACGGC TGCATAATGC GGAAACGAAT ACTCAGCCGG TCCCTACCAT CGTTACTCAA 
GAACCAAAGG ATATCCCCCT AGCAAAGCGA ACCAAGCCAC CGGTGGCCGT CATTCGATAC 
AGTCCCCAAT TAGAAGAACC CATCGCAGCA CATGAGCTGT CCACCATTCC CGAAGTCGAA 
ACTCCGCTTG CCTCCAAGAT CAACATCTCG GTAGTACCTA GCGAAAAGCT CGATCCTGCC 
GAACAACTGC TCATTGAAGT GCGCGACAAA CTTCTGGACC ACGCCCGTGC CCTTGGCTAC 
GAAGATTTTC CCAACTACCA GCAATACGTC CGAGAGCAGA ATCTGGAAGG CACCCGATAC 
GATCCGGAGA AGACCAGCTA CTCGCGCCTT CACGAACTGC TGGAAGTCAC CGACAAGAGT 
GACCACCTTC GCTATCAAAA CTTTCCCGTA CCGGCCCCGG AGATGAACGT TACTGAAGAG 
AGTGCGAATG CGAACAACAA AAAGCCCGAA TTGGAAAGCA ACTCCAGTTC CAGCACCACA 
CTCCCGGACG TGGTGGCCGA GCTCAAGCTC AGAAAGATTA TCGATAAATC TTTCGACAAC 
TCTCTTGACG ATTCCAACCT TTCGACACCG GATTCTGGTT CGTTACTAGC TCTTCATCCT 
CTTCCAGAAA AATCCCCGAT AAAATCGCAC CAGAATCAAA ACGATCCCCG CAAAACCCCC 
GACCAATCCG TCTCGGAGAC AACGGCATCC CTCGAACGGG ATCTCAATCA GCTTGGCCTG 
CGCTGGGCCT CAAGTATGCT GAAGAAAAAC CAGCAAGCCC TCCTACAAGA GCACTCCAGC 
AGCTCGTCCC TCTCACCGGC CGAAGACATC CGCCGCCTGT CGGCCAGAAA ATCCAAACCA 
CTGCCATCTC TTCGGGACGA CCCAAACGAT TCGCAGAACA CAGTCTCCGG TAAACCCTTG 
AACCTTAAAG AGTTCGTTGC CCGTGAACTG ATGATACGGA CCCATTCCGA TCCGAACTCG 
CTCTCGGATT CGTCCAGTCC GTGCAGTATG CTGTTACGAT CATTGCTGGA CATCAGCCAC 
ATTAACACCT CCACTCCGGA ATTGCTCACG CAAACCACGG ATAAGAACAT TCAGCGAACG 
TCCACTCCGG TGGCCACGAA ATCGTCCTCC GGCGGGACCC CACGGGAAAT CGCTGTCGCC 
AATGATGGCA GCCTGAATGT TACGAATGGG CTATTCTCCG GCGAATCGCG AATTTCGTCC 
GTTCACATGA GTTCTTCTTC GGGCGGGGAG AACAAACTGA CCGTTCCGAA CGTGCGCCTC 
GATGTGCACC GGTATGCAAA GGGACGGGAC ACAGCCCAGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021705269.1
CDS	558..5522
Translation	MSINITVHSSQAPSSSGGNPFANLESVRKAENDKLRKARLEQVRQQSKQLAAKVRRNFQTAKEKELANVEKLREAELKNWKQRHIAKLQADYQHCLEDVGEAHKAAEAAEQCEVWFQEKKATQQAAALQRGRQAEQKLAKDIQDREARKSKKKKEYVPTKSVAVQVSAPAEQKKDAPVQVDDSSTYANTVSAFEQFKKGSKANSVPDLRMHDRPLSPDFLSSDEELLHGIDKNKENIPTINSTTSKRIDTLYCNPTSFTSPETIQQQASAIRQQPIKPFTQVSEFVKRRREEKLHQHAHPHEPITKDIPTYCSQKNVQFDDLSENTLSFPTSTALDRSILESPRKPVRKLVEQPKVIPLKGKPSPSKRSTTDSNSAGATSSKVQYYDYNTKYRKEYDQPSAFVQRNDKPREGELNAMQEASRLERLQAEMLKARRQIPAGPDRSQTALEKLQMRKDYENLQLELDKLIKAESQAKGQDVTRKPLMTETRQRQQTEARQKRMNQAVEDLLKKRVLITCPIVRDPPRGVQLSNRPSQVNVAAGIGNAVHVPGDDSILREATNSSDSCSTIMLGYDQSNVRAGIPLPEADKVAKLKELLQKVNEQKRLLTEELKKEEEIEHELKVPIVNIKSTKTQTDNGIKKLQQRQQELEAQQKQLHEKEKEIRELEKQLKEKLNRLEKEKSKSKVPIHIETNGTGSADVQMAHDNSSTGSNDSIQSGSEIPVKIVITVNDKSKKKVKKTPKKILPEARKRKKAKDVVDRPIESQKTRGKSPKKVEAIPEKPIEVPKVHSEPPRTVPEPMEASPISSTTSTVYRQLPSKIDNRAGQLLKQLAQQQAPPPKPVKSQRRESAPKHQLKKTTPPPARPAPIAQAQQRGQNFNPNLMQYIVRLLGMSRHSIEQLGVSSSTTVSTPHDSVTNVSANQSSSVEEQTSRDQVRMDKLRRFIDENYNFLQEIDETLKEQELSGTADDNISRVEDVWMKTLSRKEKEVRREKPQIAAPPLGQSPPVRDSSLKSILKSPKKTPAKVATIMTPQGNVEVIDLTDRAEQEVLEKYSHLAENCSKRISELSDMIQKVREEKKKLIENSLSSSEQPESSTKYLDLPRSQQKAPAAETSPQSAKEDPVSEEINNIFTKSRQIGLSKDSGIAMSRPVTSSDYRDSPDARPPNAGAPQFESSEELSSREMAPTGSVAFEPLLKDIPKVSPRLHNAETNTQPVPTIVTQEPKDIPLAKRTKPPVAVIRYSPQLEEPIAAHELSTIPEVETPLASKINISVVPSEKLDPAEQLLIEVRDKLLDHARALGYEDFPNYQQYVREQNLEGTRYDPEKTSYSRLHELLEVTDKSDHLRYQNFPVPAPEMNVTEESANANNKKPELESNSSSSTTLPDVVAELKLRKIIDKSFDNSLDDSNLSTPDSGSLLALHPLPEKSPIKSHQNQNDPRKTPDQSVSETTASLERDLNQLGLRWASSMLKKNQQALLQEHSSSSSLSPAEDIRRLSARKSKPLPSLRDDPNDSQNTVSGKPLNLKEFVARELMIRTHSDPNSLSDSSSPCSMLLRSLLDISHINTSTPELLTQTTDKNIQRTSTPVATKSSSGGTPREIAVANDGSLNVTNGLFSGESRISSVHMSSSSGGENKLTVPNVRLDVHRYAKGRDTAQK

Target ORF information:

RefSeq Version	XM_021849577.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti titin (LOC5566154), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021849577.1

ORF Insert Sequence:

ATGTCCATCA ACATTACGGT GCACAGTTCG CAGGCTCCGT CTTCTTCCGG TGGGAATCCG 
TTTGCCAACT TGGAAAGCGT TCGTAAGGCC GAAAATGACA AACTGCGGAA AGCCCGATTG 
GAACAGGTCC GACAGCAGTC GAAGCAACTG GCGGCGAAAG TTCGTCGAAA CTTCCAAACT 
GCCAAGGAGA AAGAGTTGGC CAATGTGGAG AAACTGAGGG AAGCGGAATT GAAGAACTGG 
AAGCAGAGGC ACATAGCGAA GCTGCAGGCC GATTATCAAC ACTGTTTGGA GGATGTCGGT 
GAGGCGCATA AAGCTGCGGA AGCGGCTGAG CAGTGTGAAG TTTGGTTCCA GGAGAAAAAG 
GCCACCCAAC AGGCGGCAGC TCTTCAGAGG GGACGGCAAG CGGAGCAAAA ACTAGCGAAG 
GACATTCAGG ACCGAGAAGC TAGGAAGTCG AAGAAAAAGA AAGAGTATGT TCCGACCAAA 
TCGGTTGCGG TGCAAGTTTC GGCTCCCGCG GAGCAGAAAA AGGATGCCCC CGTGCAGGTG 
GATGATAGTA GTACCTACGC CAATACTGTG AGTGCATTTG AGCAGTTCAA GAAAGGTTCG 
AAGGCAAACT CGGTGCCGGA TCTACGCATG CATGACCGAC CGTTGTCACC GGATTTCCTG 
TCGTCCGATG AAGAGCTACT CCATGGTATT GATAAGAACA AAGAGAACAT TCCAACTATC 
AATTCGACAA CCAGCAAACG AATAGACACA CTTTACTGCA ATCCAACATC ATTTACCTCA 
CCAGAAACAA TTCAACAGCA AGCGTCCGCA ATCCGACAGC AACCGATAAA ACCTTTCACG 
CAGGTATCGG AGTTCGTCAA ACGACGCAGG GAAGAGAAGT TACACCAACA CGCACACCCA 
CACGAACCCA TCACGAAGGA CATCCCCACA TACTGCTCCC AAAAAAATGT GCAATTCGAT 
GACCTCAGTG AGAACACTTT ATCCTTTCCC ACGAGCACAG CCCTGGATCG ATCCATCTTG 
GAATCGCCAC GCAAACCGGT CCGAAAGCTG GTCGAACAGC CCAAGGTCAT TCCTCTCAAG 
GGCAAACCAA GTCCGTCGAA GCGATCGACG ACGGATTCCA ATTCGGCCGG AGCCACTTCC 
TCCAAGGTGC AGTACTACGA CTACAACACC AAGTACCGGA AGGAATACGA CCAACCGAGT 
GCGTTTGTGC AGCGCAATGA CAAACCAAGG GAAGGGGAGC TGAATGCCAT GCAGGAGGCC 
AGCCGACTGG AGCGGCTGCA GGCGGAGATG CTGAAAGCGA GAAGACAAAT TCCAGCGGGG 
CCGGATCGCT CGCAGACAGC GCTGGAGAAG CTACAGATGC GAAAGGACTA CGAGAACCTT 
CAGTTAGAAC TGGACAAATT GATCAAAGCT GAGTCTCAAG CCAAGGGCCA GGATGTGACA 
AGAAAACCAC TAATGACCGA GACCCGCCAG CGGCAGCAAA CGGAAGCCCG CCAGAAGCGA 
ATGAATCAAG CCGTGGAAGA TTTACTGAAG AAGCGAGTGC TGATCACTTG TCCCATAGTT 
CGGGATCCAC CCCGAGGAGT TCAGTTGTCA AATAGACCTT CACAGGTAAA CGTTGCGGCG 
GGGATTGGGA ATGCTGTCCA TGTGCCCGGA GATGATTCCA TCCTGCGGGA AGCAACAAAC 
AGCTCAGACA GTTGCAGCAC TATCATGCTG GGTTACGATC AAAGTAACGT GCGCGCGGGA 
ATTCCCCTAC CGGAAGCTGA TAAAGTCGCT AAGCTGAAGG AACTGCTGCA GAAGGTGAAC 
GAACAGAAAC GACTACTAAC GGAGGAGCTT AAGAAAGAGG AGGAAATCGA ACATGAACTG 
AAGGTGCCTA TTGTGAATAT CAAATCGACG AAAACCCAAA CGGATAACGG AATCAAGAAG 
TTGCAACAAC GACAGCAGGA ACTTGAAGCC CAGCAAAAGC AACTGCACGA GAAGGAAAAG 
GAAATTCGTG AGTTGGAGAA GCAGTTGAAG GAGAAGTTGA ATCGGTTGGA GAAAGAAAAG 
TCCAAGTCAA AGGTTCCGAT TCACATAGAA ACGAACGGTA CCGGAAGTGC AGATGTCCAA 
ATGGCTCATG ATAACAGCTC AACTGGATCG AATGATAGCA TTCAATCGGG TTCAGAAATA 
CCGGTAAAAA TTGTTATAAC TGTGAATGAT AAATCTAAAA AGAAAGTCAA GAAGACACCG 
AAGAAGATAC TCCCGGAAGC TAGGAAAAGG AAGAAAGCTA AGGATGTCGT TGATCGACCT 
ATAGAATCAC AGAAAACTAG AGGCAAGTCT CCAAAGAAGG TGGAAGCAAT CCCCGAAAAG 
CCCATTGAAG TTCCAAAAGT TCACTCAGAA CCACCGAGAA CAGTTCCAGA ACCGATGGAA 
GCGTCACCAA TCAGCTCAAC GACCTCAACC GTTTATCGAC AACTGCCATC AAAAATCGAC 
AATCGCGCCG GCCAGCTCTT GAAGCAATTG GCCCAACAAC AAGCACCGCC ACCGAAGCCT 
GTAAAATCCC AACGCCGGGA ATCTGCGCCC AAGCATCAAC TAAAAAAGAC CACCCCTCCT 
CCTGCTCGAC CAGCACCAAT TGCTCAAGCC CAGCAGCGAG GGCAAAATTT CAATCCCAAC 
CTCATGCAGT ACATCGTTCG ATTGTTGGGT ATGTCCCGGC ATTCAATCGA GCAACTCGGG 
GTCAGTTCAT CAACGACGGT TTCCACGCCG CACGATTCCG TTACCAACGT CAGCGCCAAT 
CAATCCAGCA GCGTCGAAGA GCAAACCAGT CGCGATCAGG TGCGAATGGA CAAGCTGAGA 
CGATTCATCG ACGAAAACTA TAACTTCCTC CAGGAAATCG ACGAAACCCT CAAAGAGCAG 
GAGCTGAGCG GAACGGCCGA TGATAACATC AGTCGAGTGG AGGACGTTTG GATGAAAACG 
CTCAGTCGGA AGGAGAAGGA AGTTCGACGA GAAAAACCGC AAATTGCGGC ACCTCCCCTC 
GGCCAATCAC CCCCAGTCAG GGACAGTTCT CTCAAGTCGA TTCTTAAGTC ACCTAAAAAG 
ACACCCGCCA AGGTGGCGAC AATCATGACC CCGCAAGGAA ACGTGGAAGT AATCGACCTA 
ACAGACCGTG CTGAACAAGA AGTGCTGGAA AAGTACTCGC ATCTGGCGGA AAACTGTAGC 
AAGCGAATCT CCGAATTGTC GGACATGATC CAGAAGGTCC GGGAGGAGAA GAAAAAGCTC 
ATCGAAAACA GTTTGTCATC CAGCGAGCAG CCGGAATCGT CCACCAAATA TCTGGATTTG 
CCACGATCGC AACAGAAAGC GCCCGCTGCC GAAACGAGTC CGCAGTCGGC CAAGGAGGAT 
CCCGTCAGCG AAGAGATCAA CAACATTTTC ACCAAGTCGA GACAGATTGG ACTCAGCAAG 
GATAGTGGCA TAGCAATGTC ACGACCGGTG ACATCATCAG ACTATCGTGA CAGCCCGGAC 
GCAAGACCGC CCAACGCCGG GGCACCACAG TTCGAGTCGT CCGAGGAGCT GAGCTCACGC 
GAGATGGCAC CCACTGGTTC GGTGGCGTTT GAACCCTTGC TCAAGGATAT TCCGAAGGTT 
TCACCACGGC TGCATAATGC GGAAACGAAT ACTCAGCCGG TCCCTACCAT CGTTACTCAA 
GAACCAAAGG ATATCCCCCT AGCAAAGCGA ACCAAGCCAC CGGTGGCCGT CATTCGATAC 
AGTCCCCAAT TAGAAGAACC CATCGCAGCA CATGAGCTGT CCACCATTCC CGAAGTCGAA 
ACTCCGCTTG CCTCCAAGAT CAACATCTCG GTAGTACCTA GCGAAAAGCT CGATCCTGCC 
GAACAACTGC TCATTGAAGT GCGCGACAAA CTTCTGGACC ACGCCCGTGC CCTTGGCTAC 
GAAGATTTTC CCAACTACCA GCAATACGTC CGAGAGCAGA ATCTGGAAGG CACCCGATAC 
GATCCGGAGA AGACCAGCTA CTCGCGCCTT CACGAACTGC TGGAAGTCAC CGACAAGAGT 
GACCACCTTC GCTATCAAAA CTTTCCCGTA CCGGCCCCGG AGATGAACGT TACTGAAGAG 
AGTGCGAATG CGAACAACAA AAAGCCCGAA TTGGAAAGCA ACTCCAGTTC CAGCACCACA 
CTCCCGGACG TGGTGGCCGA GCTCAAGCTC AGAAAGATTA TCGATAAATC TTTCGACAAC 
TCTCTTGACG ATTCCAACCT TTCGACACCG GATTCTGGTT CGTTACTAGC TCTTCATCCT 
CTTCCAGAAA AATCCCCGAT AAAATCGCAC CAGAATCAAA ACGATCCCCG CAAAACCCCC 
GACCAATCCG TCTCGGAGAC AACGGCATCC CTCGAACGGG ATCTCAATCA GCTTGGCCTG 
CGCTGGGCCT CAAGTATGCT GAAGAAAAAC CAGCAAGCCC TCCTACAAGA GCACTCCAGC 
AGCTCGTCCC TCTCACCGGC CGAAGACATC CGCCGCCTGT CGGCCAGAAA ATCCAAACCA 
CTGCCATCTC TTCGGGACGA CCCAAACGAT TCGCAGAACA CAGTCTCCGG TAAACCCTTG 
AACCTTAAAG AGTTCGTTGC CCGTGAACTG ATGATACGGA CCCATTCCGA TCCGAACTCG 
CTCTCGGATT CGTCCAGTCC GTGCAGTATG CTGTTACGAT CATTGCTGGA CATCAGCCAC 
ATTAACACCT CCACTCCGGA ATTGCTCACG CAAACCACGG ATAAGAACAT TCAGCGAACG 
TCCACTCCGG TGGCCACGAA ATCGTCCTCC GGCGGGACCC CACGGGAAAT CGCTGTCGCC 
AATGATGGCA GCCTGAATGT TACGAATGGG CTATTCTCCG GCGAATCGCG AATTTCGTCC 
GTTCACATGA GTTCTTCTTC GGGCGGGGAG AACAAACTGA CCGTTCCGAA CGTGCGCCTC 
GATGTGCACC GGTATGCAAA GGGACGGGAC ACAGCCCAGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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AAEL_AAEL005234 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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