LOC5566099 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC5566099
Entrez Gene ID	5566099
Full Name	spectrin alpha chain
Synonyms	AAEL015065
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021843517.1	XP_021699209.1	spectrin alpha chain isoform X1
XM_021843519.1	XP_021699211.1	spectrin alpha chain isoform X3
XM_001650415.1	XP_001650465.1	AAEL015065-PA
XM_001650415.2	XP_001650465.1	spectrin alpha chain isoform X4
XM_021843518.1	XP_021699210.1	spectrin alpha chain isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC5566099 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC5566099 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe02231	XM_001650415.1 Latest version!	Aedes aegypti AAEL015065-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAe121121	XM_021843517.1 Latest version!	Aedes aegypti spectrin alpha chain (LOC5566099), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAe121122	XM_021843518.1 Latest version!	Aedes aegypti spectrin alpha chain (LOC5566099), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAe121123	XM_021843519.1 Latest version!	Aedes aegypti spectrin alpha chain (LOC5566099), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAe121124	XM_001650415.2 Latest version!	Aedes aegypti spectrin alpha chain (LOC5566099), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC5566099 ( XM_001650415.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001650465.1 Aedes aegypti AAEL015065-RA partial mRNA.

Price & Availability↑

CloneID	OAe02231
Clone ID Related Accession (Same CDS sequence)	XM_001650415.1
Accession Version	XM_001650415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL015065-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001810287). COMPLETENESS: incomplete on the 5' end.

ATGGAACAGT TTACCCCCAA GGAGGTAAAA ATCCTCGAAA GCGCTGAGGA TATTCAAGAG 
CGCCGTGAAC AGGTGCTCAA CCGATACAAT GAGTTCAAGG TCGAAACGCG CCACAAGCGC 
GAGAAGCTGG AAGATTCGCG TCGTTTCCAG TACTTTAAGC GTGACTCGGA CGAGTTGGAA 
AGCTGGATCC ACGAGAAATT GCAGGCCGCA TCGGAGGAGA GCTATCGCGA TCCTACCAAT 
TTGCAGGCCA AGATTCAGAA GCATCAGGCG TTCGAGGCTG AAGTTTCGGC CCATAGCAAT 
GCGATCGTAG TATTGGACAA CACCGGTCAG GAGATGATCA ATCAGCACCA CTTTGCCTCG 
GACACTATCC AACGTCGCTT GGATGAGTTG CACCGTTTGT GGGAATTGTT GCTGTCCCGT 
TTGGCCGAAA AGGGAATGAA ATTGCAACAG GCTCTGGTCT TGGTGCAATT CTTGCGCCAC 
TGCGAGGAGG TCATGTTTTG GATCAAAGAC AAGGAAGCTT TTGTGACGGC CGATGAGTTC 
GGTCAAGATT TGGAACATGT CGAAGTCTTG CAACGTAAGT TTGACGAGTT CCAGAAGGAC 
ATGGCTTCGC AAGAGTATCG AGTTACTGAA GTCAACGAGT TGGCTGACAA GTTGCTATCA 
AACGGACACC CTGAGCGCGA AACTATCACT CGCAAGAAGG AAGAATTGAA CGAGGCTTGG 
CAACGCCTGA AGCAGCTGGC TATTCTGCGT CAGGAAAAGC TTTTCGGTGC CCATGAAATT 
CAACGTTTCA ATCGCGATGC TGATGAAACC GTCGCTTGGA TTGCTGAAAA AGACGTTGTT 
CTGTCGTCGG ATGATTATGG ACGTGACTTG GCTAGTGTTC AAGCTTTGCA ACGCAAGCAC 
GAAGGGGTAG AGCGCGACTT GGCTGCTCTG GAAGATAAGG TGGCCACTTT GGGAGCTGAA 
GCCGGTCGCT TATGTAGCAT CCACGCCGAT CATAGTGATC AGATTCGTGA GAAGCAAGCT 
GAAATTGCTG CCTACTGGCA ATCATTGACT GCCAAGGCCA AGGAACGTAA GCAAAAACTG 
GACGAATCAT ATTTCTTGCA CCGTTTCTTG GCTGATTTCC GCGATTTGGT TTCGTGGATC 
AACGGAATGA AGGCCATTAT CTCGGCTGAT GAACTTGCAA AGGATGTAGC TGGCGCTGAG 
GCTCTACTTG AGCGTCACCA GGAGCACAAA GGAGAAATCG ATGCCCGTGG AGATAGCTTC 
AAGGTTACAA CCGAGGCTGG TCGTCAGCTT TTGGAACGGG AGCATTATGC CGCTGCTGAA 
GTGCAGGAAA AATTGGCTGC TCTAGAGAGC GATAAGAGTT CGCTTCTCGC GCTTTGGGAA 
GAACGTCGCA TTTTGTATGA ACAGTGCATG GATCTACAAC TGTTCTATCG TGACACTGAG 
CAGGCAGATA CTTGGATGGC CAAACAGGAA GCTTTCCTAG CCAATGAAGA TCTAGGAGAT 
TCGCTTGATT CCGTCGAAGC GCTGATTAAG AAGCACGAAG ATTTCGAGAA AAGTTTGGCG 
GCCCAAGAGG AGAAGATCAA GGCTTTGGAT GTTTTCGCTA CAAAGCTCAT CGATGGTCAG 
CATTACGCTG CTGACGATGT TGCCCAACGT CGTTCAATGT TGTTAGCTCG TCGATCAGCC 
TTGCTCGAGA AGTCTTCTGT ACGCCGTCAA CTGCTGGAGG ATTCCAGTGC TTTGCAGCAA 
TTTGAACGTG ACTGCGATGA GACTAAAGGA TGGATCAGCG AAAAGCTTAA ATTTGCCACA 
GATGACAGCT ATTTGGACCC AACCAATCTG AATGGAAAAG TTCAGAAACA TACCAACTTT 
GAGCATGAAT TAACGGCCAA CAAGAGCCGC ATTGAAGACA TCACCACCAA TGGTCAGAAT 
TTAGTCGAGA AGGGACACTA CGGTGCCGAT CAAATCAACT CGCGCATGCA GGAGATTGTC 
ACTTTGTGGG AATCTTTGGT TCAATCATCG GACAAAAAGG GCTGCAAATT GCAGGAAGCA 
TCCCAACAGC AACAATTCAA TCGCACCGTT GAAGATATTG AACTGTGGTT GAGCGAAGTG 
GAAGGCCAAC TAATGTCCGA GGATTATGGC AAAGACCTGA CCAGTGTCCA GAATTTGCAG 
AAGAAACATG GTTTGTTGGA GGCTGATGTC ATGGCCCATC AAGATCGCAT TGAAGGAATT 
AAAGTTGCAG CGAACAAGTT CGTCGAGAGT GGTCATTTCG ATGCCGACAA CATCCGTAAT 
AAGGAATCTG CGCTGTCCAA GCGTTATGGA GCTCTGGCTA CTCCGATGGC TGAACGAAAG 
CAGCGTTTGT TGGATTCCCT ACAGGTGCAG CAACTGTTCC GTGATCTGGA AGACGAAGCT 
GCATGGATCC GTGAGAAGGA ACCTGTCGCT GCATCCACCA ACCGTGGCCG CGATTTAATC 
GGTGTTCAGA ATTTGATTAA GAAGCATCAG GCCGTGTTGG CAGAAATCAA CAACCATGAA 
AATCGCGTCG CCGGAGTCAT CGCGAATGGC GAGCAAATGT TGACTGAGCA GCCTTTTGCC 
ACCGAAGATA TCAAATTGCG TTTGGATGCT GTTAAAGATC AATGGAATTC ATTGAAGGAG 
AAGGCTAATC AACGGAAGCA AGATCTGGAA GATTCGTTGC AGGCCCACCA GTACTTTGCT 
GATGCTAACG AGGCGGAATC TTGGATGCGT GAGAAGGAAC CTATCGTATC GAATCAAGAT 
TACGGCAAGG ACGAAGATTC GTCTGAAGCT TTGTTGAAGA AGCATGAAGC ATTGGTGTCC 
GATTTGGAAG CTTTTGGTAA CACCATTCAA GCTTTGCAAG AACAGGCCAA GAATTGTCGT 
CAGCAGGAAA CACCAGTGGT GGACATTACC GGAAAGGAAT GCGTCATGGC TCTGTATGAT 
TATACCGAGA AATCTCCTCG TGAAGTTTCG ATGAAGAAGG GGGACGTATT AACTCTTTTG 
AACTCGAACA ACAAGGATTG GTGGAAGGTA GAGGTCAACG ATCGTCAGGG ATTCGTTCCG 
GCTGCATACA TTAAGAAGAT CGATCCTGGC CTAAGCGCCA GTCAACAGAA TTTGGTTGAT 
GGCCATTCAA TTGCTAAGCG CCAGGCTCAA ATCAACAGCC AGTACGATAA CTTGCTTGCT 
TTGGCACGTG AACGACAGAA CAAATTGAAT GAAACCGTAA AGGCTTATGT TCTGGTACGT 
GAAGCTGCTG ATTTGTCCGC TTGGATTCGC GACAAGGAAA GCCACGCTCA GATTAAGGAT 
GTTGGCGAAG ATCTGGAAGA AGTTGAAGTT ATGCAGAAGA AGTTTGATGA CTTCAATGAT 
GATCTGAAGG CTAACGAAGT TCGTCTAGCT AAGTTGAACG AGATTGCCGT ACAGCTGACT 
TCGCTAGGTC AAACCGAAGC AGCATTGAAG ATCAAAACTC AAATTCAGAC CTTGAACGAA 
GAGTGGACAG TTTTGCAGAA TATTACCCAG GAAAGGGCTA GTCAACTTGG ATCGGCTCAT 
GAGGTTCAAC GCTTCCATCG TGATGTAGAT GAAACCAAGG ATTGGATTGG CGAGAAGGAC 
ACAGCTTTGA CGAATGACGA TCTTGGTAAG GATCTGCGTG GCGTTCAGAC CCTGCAACGC 
AAGCATGAAG GTTTGGAGCG TGATTTGGCA GCTCTACGAG ACAAAATTCG TCAATTGGAC 
GAAACTGCCA ATCGCCTCAT GCAATCCCAC CCGGAAACCG CTGAGCAGAC TTATGCCAAG 
CAGAAGGAAA TCAACGAGGA ATGGCAGCAG GTTGTTGCCA AAACTCAGCA ACGCAAGGAA 
AAGCTGTTAG ATTCTTACGA TCTACAGCGG TTCCTGAGCG ATTATCGCGA TCTGATGGCT 
TGGATCAGCT CAATGATGGG CTTGGTCACG TCCGAAGAGT TGGCCAACGA TGTCACCGGG 
GCTGAAGCTT TAATCGAAAG ACATCAGGAA CACCGTACCG AGGTAGATGC CCGCGCTGGA 
ACCTTCGCTG CGTTCGATCA GTTTGGAGCT GAGCTGTTGC AGGCAAATCA TTATGCGTCA 
CCCGAAATTC AAGAGAAAAT TGAAAACTTG GCCAAGGCAC GTGAGGATTT GGAGCGTGCC 
TGGACTGCTC GTCGTCTGCA GTTGGACCAG AATCTGGATC TTCAGCTGTA TCTGCGTGAT 
TGCGAACAGG CTGAGAACTG GATGAGCGCC CGTGAAGCAT TCCTGAATGC CGAAGAAGTC 
GACTCCAAGG GCGACAACGT GGAAGCATTG ATTAAGAAGC ACGAAGACTT TGACAAAGCT 
ATCAACGGTC ATGAGGAGAA AATTGCCGCC CTGCAGGTGC TGGCTGATCA ATTGATTGCC 
CAGGAGCACT ACGCAGGAAA ATTAATCGAT GACAAGCGTG CAGATGTGTT GGATCGTTGG 
CGTCACTTGA AGGAAGATTT GATTGAAAAA CGATCTCGCT TGGGAGATGA GCAAACTCTA 
CAGCAATTCT CACGTGATGC TGATGAAATC GAAAACTGGA TTGCCGAGAA GCTGCAACTA 
GCCACGGAGG AAAGTTACAA GGACCCGGCC AATATTCAAT CCAAGCATCA GAAGCACCAG 
GCTTTCGAAG CCGAATTGGC TGCTAATGCC GATCGTATTC AGAGCGTCTT GGCGATGGGA 
AGCAATTTGA TCGATAAGAA TCAATGCAGT GGGTCCGAAG ATGCAGTTCA GAAGCGTTTG 
ACACAGATCG CTGATCAGTG GGAATATCTG ACCCAGAAAA CAACGGAGAA ATCCCTGAAG 
CTGAAGGAAG CTAACAAACA GCGCACTTAT ATTGCTGCTG TCAAGGATTT GGACTTCTGG 
TTGGGTGAAG TTGAGAGTCT TTTGACCTCA GAAGATGCTG GAAAGGATTT GGCTTCCGTC 
CAAAATCTTA TGAAGAAGCA CCAGCTGGTA GAGGCTGACA TCCATGCCCA TGAAGATCGC 
ATCAAGGATA TGAACGCCCA AGCCGACTCA TTGGTCGAAA GTGGCCAATT TGACAGTGCA 
GGAATTCAAG AAAAGCGTCA GTCCATCAAC GAGCGTTACG AACGTATTTG TAATTTGGCA 
GCACATCGCC AAGCTCGCTT GAATGAAGCC AACACTTTGC ATCAATTCTT CCGTGATATT 
GCCGACGAGG AATCCTGGAT TAAGGAAAAG AAATTGTTGG TTGGTTCGGA TGATTATGGT 
CGCGATTTAA CCGGTGTCCA AAATCTGAAA AAGAAGCACA AGCGTCTTGA GGCTGAGTTG 
GCTTCTCACG AGCCAGCAAT TCAGGCTGTT CAGGAAGCTG GTGAAAAGTT GATGGATGTT 
TCGAATCTTG GTGTTCCAGA AATTGAGCAA CGTCTGAAGG CTCTGAACCA AGCCTGGGCT 
GAATTGAAGA GCTTGGCTGC TACTCGTGGT CAGAAACTGG ATGAATCTCT CATTTATCAA 
CAATTCTTAG CTAAGGTTGA AGAAGAGGAA GCATGGATTA CCGAGAAGCA ACAACTGTTG 
TCGGTTGAAG ATTATGGAGA TTCGATGGCT GCTGTCCAGG GTCTTTTGAA GAAACATGAC 
GCCTTCGAGA CCGATTTCGC CGCTCATCGT GATCGTTGCG CTGACATTCA TGACAATGGT 
CAAACTTTGG TGACCAATAA TAATCATCAC GCCGAAAGCA TTTCGCAGCG CTGCACACAA 
CTGGATAAGA AGTTGGAGAA TCTGCAGGCG TTGGCTACTC GTCGCAAGAG CGCCCTTCTG 
GACAACTTTG CTTATCTTCA ATTCATGTGG AAGGCCGATG TGGTTGAAAG TTGGATTGCC 
GATAAAGAAA ATCACGTCAA ATCGGAAGAG TTCGGACGTG ATTTGTCTAC TGTTCAAACA 
TTGCTAACCA AACAGGAGAC ATTCGACGCT GGTCTCTCTG CATTCGAACA TGAAGGAATC 
CATAATATCA CTGCGTTGAA AGATCAACTA ATTAACGCCA ATCACGCCCA ATCGGCCGCC 
ATCCTCAAGC GCCACGAAGA TGTGTTGACC CGCTGGCAGA AATTGCGCGC TGATTCGGAG 
GCTCGCAAGT ATCGTCTATT GGCCATGCAG GAGCAGTTCC GTCAGATTGA AGATCTCTAT 
CTGACGTTTG CCAAGAAGGC TTCCGCTTTC AACTCGTGGT TCGAAAACGC TGAAGAAGAT 
CTCACCGATC CGGTGCGGTG CAATTCGATT GAGGAAATCA AGGCGCTGCG CGAAGCTCAC 
GCCCAGTTCC AGGCATCGTT GTCATCAGCC CAGTATGACT TCCAAGCATT GGCCGATCTG 
GACCGTAAAA TTAAGAGCTT CAACGTTGGT CCCAATCCGT ACACGTGGTT CACAATGGAA 
GCTCTAGAAG ACACCTGGCG TAATCTGCAA AAGATCATCG AAGAACGTGA TGCTGAATTG 
GCAAAGGAAG TTCACCGTCA AGAAGAGAAC GACAAGCTTC GCAAGGAATT CGCCAAACAT 
GCCAACTTGT TCCACCAGTG GCTTACCGAA ACAAGAACAT CTCTTATGGA CGGATCCGGT 
TCGTTGGAGG AACAATTTGA AGCGCTCTGT CACAAAGCAA ATGAAATTCG CGCTCGTCGT 
GGTGATCTGA AGAAGATTGA AGAGTTGGGT GCCACCTTGG AAGAGCACCT TATTTTGGAT 
AACCGCTATA CTGAACACTC AACTGTAGGC CTTGCACAAC AGTGGGATCA GCTTGACCAA 
CTTGCCATGC GCATGCAGCA CAACCTGAGA CAACAAATCC AAGCCCGCAA TCAATCTGGC 
GTCTCGGAGG ATTCACTCAA AGAATTCTCA ATGATGTTCA AGCACTTCGA CAAGGACAAG 
AGCGGCAAGC TCAACCACCA AGAATTCAAA TCCTGTTTAC GTGCCCTGGG TTACGATTTA 
CCAATGGTGG AAGAAGGCCA GCCCGACCCG GAGTTCGAGG AAATTTTGAA TGTGGTGGAC 
CCGAACCGCG ATGGACATGT TTCACTCCAG GAATACATAG CCTTTATGAT CTCCAAGGAA 
ACGGAGAACG TACAAAGCTA CGAGGAAATC GAAAATGCTT TCCGCGCAAT CACCGCCTCC 
GATCGTCCTT ACGTCACCAA GGATGAACTT TATTCCAACC TCACCAAGGA CATGGCAGAT 
TACTGCGTAC AGCGGATGAA ACCGTTCAAC GACCCGAAAA CGGGTCATCC CATTACCGGT 
GCCCTCGATT ATGTGGAGTT CACGCGAACT CTATTCCAAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001650465.1
CDS	1..7245
Translation	MEQFTPKEVKILESAEDIQERREQVLNRYNEFKVETRHKREKLEDSRRFQYFKRDSDELESWIHEKLQAASEESYRDPTNLQAKIQKHQAFEAEVSAHSNAIVVLDNTGQEMINQHHFASDTIQRRLDELHRLWELLLSRLAEKGMKLQQALVLVQFLRHCEEVMFWIKDKEAFVTADEFGQDLEHVEVLQRKFDEFQKDMASQEYRVTEVNELADKLLSNGHPERETITRKKEELNEAWQRLKQLAILRQEKLFGAHEIQRFNRDADETVAWIAEKDVVLSSDDYGRDLASVQALQRKHEGVERDLAALEDKVATLGAEAGRLCSIHADHSDQIREKQAEIAAYWQSLTAKAKERKQKLDESYFLHRFLADFRDLVSWINGMKAIISADELAKDVAGAEALLERHQEHKGEIDARGDSFKVTTEAGRQLLEREHYAAAEVQEKLAALESDKSSLLALWEERRILYEQCMDLQLFYRDTEQADTWMAKQEAFLANEDLGDSLDSVEALIKKHEDFEKSLAAQEEKIKALDVFATKLIDGQHYAADDVAQRRSMLLARRSALLEKSSVRRQLLEDSSALQQFERDCDETKGWISEKLKFATDDSYLDPTNLNGKVQKHTNFEHELTANKSRIEDITTNGQNLVEKGHYGADQINSRMQEIVTLWESLVQSSDKKGCKLQEASQQQQFNRTVEDIELWLSEVEGQLMSEDYGKDLTSVQNLQKKHGLLEADVMAHQDRIEGIKVAANKFVESGHFDADNIRNKESALSKRYGALATPMAERKQRLLDSLQVQQLFRDLEDEAAWIREKEPVAASTNRGRDLIGVQNLIKKHQAVLAEINNHENRVAGVIANGEQMLTEQPFATEDIKLRLDAVKDQWNSLKEKANQRKQDLEDSLQAHQYFADANEAESWMREKEPIVSNQDYGKDEDSSEALLKKHEALVSDLEAFGNTIQALQEQAKNCRQQETPVVDITGKECVMALYDYTEKSPREVSMKKGDVLTLLNSNNKDWWKVEVNDRQGFVPAAYIKKIDPGLSASQQNLVDGHSIAKRQAQINSQYDNLLALARERQNKLNETVKAYVLVREAADLSAWIRDKESHAQIKDVGEDLEEVEVMQKKFDDFNDDLKANEVRLAKLNEIAVQLTSLGQTEAALKIKTQIQTLNEEWTVLQNITQERASQLGSAHEVQRFHRDVDETKDWIGEKDTALTNDDLGKDLRGVQTLQRKHEGLERDLAALRDKIRQLDETANRLMQSHPETAEQTYAKQKEINEEWQQVVAKTQQRKEKLLDSYDLQRFLSDYRDLMAWISSMMGLVTSEELANDVTGAEALIERHQEHRTEVDARAGTFAAFDQFGAELLQANHYASPEIQEKIENLAKAREDLERAWTARRLQLDQNLDLQLYLRDCEQAENWMSAREAFLNAEEVDSKGDNVEALIKKHEDFDKAINGHEEKIAALQVLADQLIAQEHYAGKLIDDKRADVLDRWRHLKEDLIEKRSRLGDEQTLQQFSRDADEIENWIAEKLQLATEESYKDPANIQSKHQKHQAFEAELAANADRIQSVLAMGSNLIDKNQCSGSEDAVQKRLTQIADQWEYLTQKTTEKSLKLKEANKQRTYIAAVKDLDFWLGEVESLLTSEDAGKDLASVQNLMKKHQLVEADIHAHEDRIKDMNAQADSLVESGQFDSAGIQEKRQSINERYERICNLAAHRQARLNEANTLHQFFRDIADEESWIKEKKLLVGSDDYGRDLTGVQNLKKKHKRLEAELASHEPAIQAVQEAGEKLMDVSNLGVPEIEQRLKALNQAWAELKSLAATRGQKLDESLIYQQFLAKVEEEEAWITEKQQLLSVEDYGDSMAAVQGLLKKHDAFETDFAAHRDRCADIHDNGQTLVTNNNHHAESISQRCTQLDKKLENLQALATRRKSALLDNFAYLQFMWKADVVESWIADKENHVKSEEFGRDLSTVQTLLTKQETFDAGLSAFEHEGIHNITALKDQLINANHAQSAAILKRHEDVLTRWQKLRADSEARKYRLLAMQEQFRQIEDLYLTFAKKASAFNSWFENAEEDLTDPVRCNSIEEIKALREAHAQFQASLSSAQYDFQALADLDRKIKSFNVGPNPYTWFTMEALEDTWRNLQKIIEERDAELAKEVHRQEENDKLRKEFAKHANLFHQWLTETRTSLMDGSGSLEEQFEALCHKANEIRARRGDLKKIEELGATLEEHLILDNRYTEHSTVGLAQQWDQLDQLAMRMQHNLRQQIQARNQSGVSEDSLKEFSMMFKHFDKDKSGKLNHQEFKSCLRALGYDLPMVEEGQPDPEFEEILNVVDPNRDGHVSLQEYIAFMISKETENVQSYEEIENAFRAITASDRPYVTKDELYSNLTKDMADYCVQRMKPFNDPKTGHPITGALDYVEFTRTLFQN

Target ORF information:

RefSeq Version	XM_001650415.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL015065-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001650415.1

ORF Insert Sequence:

ATGGAACAGT TTACCCCCAA GGAGGTAAAA ATCCTCGAAA GCGCTGAGGA TATTCAAGAG 
CGCCGTGAAC AGGTGCTCAA CCGATACAAT GAGTTCAAGG TCGAAACGCG CCACAAGCGC 
GAGAAGCTGG AAGATTCGCG TCGTTTCCAG TACTTTAAGC GTGACTCGGA CGAGTTGGAA 
AGCTGGATCC ACGAGAAATT GCAGGCCGCA TCGGAGGAGA GCTATCGCGA TCCTACCAAT 
TTGCAGGCCA AGATTCAGAA GCATCAGGCG TTCGAGGCTG AAGTTTCGGC CCATAGCAAT 
GCGATCGTAG TATTGGACAA CACCGGTCAG GAGATGATCA ATCAGCACCA CTTTGCCTCG 
GACACTATCC AACGTCGCTT GGATGAGTTG CACCGTTTGT GGGAATTGTT GCTGTCCCGT 
TTGGCCGAAA AGGGAATGAA ATTGCAACAG GCTCTGGTCT TGGTGCAATT CTTGCGCCAC 
TGCGAGGAGG TCATGTTTTG GATCAAAGAC AAGGAAGCTT TTGTGACGGC CGATGAGTTC 
GGTCAAGATT TGGAACATGT CGAAGTCTTG CAACGTAAGT TTGACGAGTT CCAGAAGGAC 
ATGGCTTCGC AAGAGTATCG AGTTACTGAA GTCAACGAGT TGGCTGACAA GTTGCTATCA 
AACGGACACC CTGAGCGCGA AACTATCACT CGCAAGAAGG AAGAATTGAA CGAGGCTTGG 
CAACGCCTGA AGCAGCTGGC TATTCTGCGT CAGGAAAAGC TTTTCGGTGC CCATGAAATT 
CAACGTTTCA ATCGCGATGC TGATGAAACC GTCGCTTGGA TTGCTGAAAA AGACGTTGTT 
CTGTCGTCGG ATGATTATGG ACGTGACTTG GCTAGTGTTC AAGCTTTGCA ACGCAAGCAC 
GAAGGGGTAG AGCGCGACTT GGCTGCTCTG GAAGATAAGG TGGCCACTTT GGGAGCTGAA 
GCCGGTCGCT TATGTAGCAT CCACGCCGAT CATAGTGATC AGATTCGTGA GAAGCAAGCT 
GAAATTGCTG CCTACTGGCA ATCATTGACT GCCAAGGCCA AGGAACGTAA GCAAAAACTG 
GACGAATCAT ATTTCTTGCA CCGTTTCTTG GCTGATTTCC GCGATTTGGT TTCGTGGATC 
AACGGAATGA AGGCCATTAT CTCGGCTGAT GAACTTGCAA AGGATGTAGC TGGCGCTGAG 
GCTCTACTTG AGCGTCACCA GGAGCACAAA GGAGAAATCG ATGCCCGTGG AGATAGCTTC 
AAGGTTACAA CCGAGGCTGG TCGTCAGCTT TTGGAACGGG AGCATTATGC CGCTGCTGAA 
GTGCAGGAAA AATTGGCTGC TCTAGAGAGC GATAAGAGTT CGCTTCTCGC GCTTTGGGAA 
GAACGTCGCA TTTTGTATGA ACAGTGCATG GATCTACAAC TGTTCTATCG TGACACTGAG 
CAGGCAGATA CTTGGATGGC CAAACAGGAA GCTTTCCTAG CCAATGAAGA TCTAGGAGAT 
TCGCTTGATT CCGTCGAAGC GCTGATTAAG AAGCACGAAG ATTTCGAGAA AAGTTTGGCG 
GCCCAAGAGG AGAAGATCAA GGCTTTGGAT GTTTTCGCTA CAAAGCTCAT CGATGGTCAG 
CATTACGCTG CTGACGATGT TGCCCAACGT CGTTCAATGT TGTTAGCTCG TCGATCAGCC 
TTGCTCGAGA AGTCTTCTGT ACGCCGTCAA CTGCTGGAGG ATTCCAGTGC TTTGCAGCAA 
TTTGAACGTG ACTGCGATGA GACTAAAGGA TGGATCAGCG AAAAGCTTAA ATTTGCCACA 
GATGACAGCT ATTTGGACCC AACCAATCTG AATGGAAAAG TTCAGAAACA TACCAACTTT 
GAGCATGAAT TAACGGCCAA CAAGAGCCGC ATTGAAGACA TCACCACCAA TGGTCAGAAT 
TTAGTCGAGA AGGGACACTA CGGTGCCGAT CAAATCAACT CGCGCATGCA GGAGATTGTC 
ACTTTGTGGG AATCTTTGGT TCAATCATCG GACAAAAAGG GCTGCAAATT GCAGGAAGCA 
TCCCAACAGC AACAATTCAA TCGCACCGTT GAAGATATTG AACTGTGGTT GAGCGAAGTG 
GAAGGCCAAC TAATGTCCGA GGATTATGGC AAAGACCTGA CCAGTGTCCA GAATTTGCAG 
AAGAAACATG GTTTGTTGGA GGCTGATGTC ATGGCCCATC AAGATCGCAT TGAAGGAATT 
AAAGTTGCAG CGAACAAGTT CGTCGAGAGT GGTCATTTCG ATGCCGACAA CATCCGTAAT 
AAGGAATCTG CGCTGTCCAA GCGTTATGGA GCTCTGGCTA CTCCGATGGC TGAACGAAAG 
CAGCGTTTGT TGGATTCCCT ACAGGTGCAG CAACTGTTCC GTGATCTGGA AGACGAAGCT 
GCATGGATCC GTGAGAAGGA ACCTGTCGCT GCATCCACCA ACCGTGGCCG CGATTTAATC 
GGTGTTCAGA ATTTGATTAA GAAGCATCAG GCCGTGTTGG CAGAAATCAA CAACCATGAA 
AATCGCGTCG CCGGAGTCAT CGCGAATGGC GAGCAAATGT TGACTGAGCA GCCTTTTGCC 
ACCGAAGATA TCAAATTGCG TTTGGATGCT GTTAAAGATC AATGGAATTC ATTGAAGGAG 
AAGGCTAATC AACGGAAGCA AGATCTGGAA GATTCGTTGC AGGCCCACCA GTACTTTGCT 
GATGCTAACG AGGCGGAATC TTGGATGCGT GAGAAGGAAC CTATCGTATC GAATCAAGAT 
TACGGCAAGG ACGAAGATTC GTCTGAAGCT TTGTTGAAGA AGCATGAAGC ATTGGTGTCC 
GATTTGGAAG CTTTTGGTAA CACCATTCAA GCTTTGCAAG AACAGGCCAA GAATTGTCGT 
CAGCAGGAAA CACCAGTGGT GGACATTACC GGAAAGGAAT GCGTCATGGC TCTGTATGAT 
TATACCGAGA AATCTCCTCG TGAAGTTTCG ATGAAGAAGG GGGACGTATT AACTCTTTTG 
AACTCGAACA ACAAGGATTG GTGGAAGGTA GAGGTCAACG ATCGTCAGGG ATTCGTTCCG 
GCTGCATACA TTAAGAAGAT CGATCCTGGC CTAAGCGCCA GTCAACAGAA TTTGGTTGAT 
GGCCATTCAA TTGCTAAGCG CCAGGCTCAA ATCAACAGCC AGTACGATAA CTTGCTTGCT 
TTGGCACGTG AACGACAGAA CAAATTGAAT GAAACCGTAA AGGCTTATGT TCTGGTACGT 
GAAGCTGCTG ATTTGTCCGC TTGGATTCGC GACAAGGAAA GCCACGCTCA GATTAAGGAT 
GTTGGCGAAG ATCTGGAAGA AGTTGAAGTT ATGCAGAAGA AGTTTGATGA CTTCAATGAT 
GATCTGAAGG CTAACGAAGT TCGTCTAGCT AAGTTGAACG AGATTGCCGT ACAGCTGACT 
TCGCTAGGTC AAACCGAAGC AGCATTGAAG ATCAAAACTC AAATTCAGAC CTTGAACGAA 
GAGTGGACAG TTTTGCAGAA TATTACCCAG GAAAGGGCTA GTCAACTTGG ATCGGCTCAT 
GAGGTTCAAC GCTTCCATCG TGATGTAGAT GAAACCAAGG ATTGGATTGG CGAGAAGGAC 
ACAGCTTTGA CGAATGACGA TCTTGGTAAG GATCTGCGTG GCGTTCAGAC CCTGCAACGC 
AAGCATGAAG GTTTGGAGCG TGATTTGGCA GCTCTACGAG ACAAAATTCG TCAATTGGAC 
GAAACTGCCA ATCGCCTCAT GCAATCCCAC CCGGAAACCG CTGAGCAGAC TTATGCCAAG 
CAGAAGGAAA TCAACGAGGA ATGGCAGCAG GTTGTTGCCA AAACTCAGCA ACGCAAGGAA 
AAGCTGTTAG ATTCTTACGA TCTACAGCGG TTCCTGAGCG ATTATCGCGA TCTGATGGCT 
TGGATCAGCT CAATGATGGG CTTGGTCACG TCCGAAGAGT TGGCCAACGA TGTCACCGGG 
GCTGAAGCTT TAATCGAAAG ACATCAGGAA CACCGTACCG AGGTAGATGC CCGCGCTGGA 
ACCTTCGCTG CGTTCGATCA GTTTGGAGCT GAGCTGTTGC AGGCAAATCA TTATGCGTCA 
CCCGAAATTC AAGAGAAAAT TGAAAACTTG GCCAAGGCAC GTGAGGATTT GGAGCGTGCC 
TGGACTGCTC GTCGTCTGCA GTTGGACCAG AATCTGGATC TTCAGCTGTA TCTGCGTGAT 
TGCGAACAGG CTGAGAACTG GATGAGCGCC CGTGAAGCAT TCCTGAATGC CGAAGAAGTC 
GACTCCAAGG GCGACAACGT GGAAGCATTG ATTAAGAAGC ACGAAGACTT TGACAAAGCT 
ATCAACGGTC ATGAGGAGAA AATTGCCGCC CTGCAGGTGC TGGCTGATCA ATTGATTGCC 
CAGGAGCACT ACGCAGGAAA ATTAATCGAT GACAAGCGTG CAGATGTGTT GGATCGTTGG 
CGTCACTTGA AGGAAGATTT GATTGAAAAA CGATCTCGCT TGGGAGATGA GCAAACTCTA 
CAGCAATTCT CACGTGATGC TGATGAAATC GAAAACTGGA TTGCCGAGAA GCTGCAACTA 
GCCACGGAGG AAAGTTACAA GGACCCGGCC AATATTCAAT CCAAGCATCA GAAGCACCAG 
GCTTTCGAAG CCGAATTGGC TGCTAATGCC GATCGTATTC AGAGCGTCTT GGCGATGGGA 
AGCAATTTGA TCGATAAGAA TCAATGCAGT GGGTCCGAAG ATGCAGTTCA GAAGCGTTTG 
ACACAGATCG CTGATCAGTG GGAATATCTG ACCCAGAAAA CAACGGAGAA ATCCCTGAAG 
CTGAAGGAAG CTAACAAACA GCGCACTTAT ATTGCTGCTG TCAAGGATTT GGACTTCTGG 
TTGGGTGAAG TTGAGAGTCT TTTGACCTCA GAAGATGCTG GAAAGGATTT GGCTTCCGTC 
CAAAATCTTA TGAAGAAGCA CCAGCTGGTA GAGGCTGACA TCCATGCCCA TGAAGATCGC 
ATCAAGGATA TGAACGCCCA AGCCGACTCA TTGGTCGAAA GTGGCCAATT TGACAGTGCA 
GGAATTCAAG AAAAGCGTCA GTCCATCAAC GAGCGTTACG AACGTATTTG TAATTTGGCA 
GCACATCGCC AAGCTCGCTT GAATGAAGCC AACACTTTGC ATCAATTCTT CCGTGATATT 
GCCGACGAGG AATCCTGGAT TAAGGAAAAG AAATTGTTGG TTGGTTCGGA TGATTATGGT 
CGCGATTTAA CCGGTGTCCA AAATCTGAAA AAGAAGCACA AGCGTCTTGA GGCTGAGTTG 
GCTTCTCACG AGCCAGCAAT TCAGGCTGTT CAGGAAGCTG GTGAAAAGTT GATGGATGTT 
TCGAATCTTG GTGTTCCAGA AATTGAGCAA CGTCTGAAGG CTCTGAACCA AGCCTGGGCT 
GAATTGAAGA GCTTGGCTGC TACTCGTGGT CAGAAACTGG ATGAATCTCT CATTTATCAA 
CAATTCTTAG CTAAGGTTGA AGAAGAGGAA GCATGGATTA CCGAGAAGCA ACAACTGTTG 
TCGGTTGAAG ATTATGGAGA TTCGATGGCT GCTGTCCAGG GTCTTTTGAA GAAACATGAC 
GCCTTCGAGA CCGATTTCGC CGCTCATCGT GATCGTTGCG CTGACATTCA TGACAATGGT 
CAAACTTTGG TGACCAATAA TAATCATCAC GCCGAAAGCA TTTCGCAGCG CTGCACACAA 
CTGGATAAGA AGTTGGAGAA TCTGCAGGCG TTGGCTACTC GTCGCAAGAG CGCCCTTCTG 
GACAACTTTG CTTATCTTCA ATTCATGTGG AAGGCCGATG TGGTTGAAAG TTGGATTGCC 
GATAAAGAAA ATCACGTCAA ATCGGAAGAG TTCGGACGTG ATTTGTCTAC TGTTCAAACA 
TTGCTAACCA AACAGGAGAC ATTCGACGCT GGTCTCTCTG CATTCGAACA TGAAGGAATC 
CATAATATCA CTGCGTTGAA AGATCAACTA ATTAACGCCA ATCACGCCCA ATCGGCCGCC 
ATCCTCAAGC GCCACGAAGA TGTGTTGACC CGCTGGCAGA AATTGCGCGC TGATTCGGAG 
GCTCGCAAGT ATCGTCTATT GGCCATGCAG GAGCAGTTCC GTCAGATTGA AGATCTCTAT 
CTGACGTTTG CCAAGAAGGC TTCCGCTTTC AACTCGTGGT TCGAAAACGC TGAAGAAGAT 
CTCACCGATC CGGTGCGGTG CAATTCGATT GAGGAAATCA AGGCGCTGCG CGAAGCTCAC 
GCCCAGTTCC AGGCATCGTT GTCATCAGCC CAGTATGACT TCCAAGCATT GGCCGATCTG 
GACCGTAAAA TTAAGAGCTT CAACGTTGGT CCCAATCCGT ACACGTGGTT CACAATGGAA 
GCTCTAGAAG ACACCTGGCG TAATCTGCAA AAGATCATCG AAGAACGTGA TGCTGAATTG 
GCAAAGGAAG TTCACCGTCA AGAAGAGAAC GACAAGCTTC GCAAGGAATT CGCCAAACAT 
GCCAACTTGT TCCACCAGTG GCTTACCGAA ACAAGAACAT CTCTTATGGA CGGATCCGGT 
TCGTTGGAGG AACAATTTGA AGCGCTCTGT CACAAAGCAA ATGAAATTCG CGCTCGTCGT 
GGTGATCTGA AGAAGATTGA AGAGTTGGGT GCCACCTTGG AAGAGCACCT TATTTTGGAT 
AACCGCTATA CTGAACACTC AACTGTAGGC CTTGCACAAC AGTGGGATCA GCTTGACCAA 
CTTGCCATGC GCATGCAGCA CAACCTGAGA CAACAAATCC AAGCCCGCAA TCAATCTGGC 
GTCTCGGAGG ATTCACTCAA AGAATTCTCA ATGATGTTCA AGCACTTCGA CAAGGACAAG 
AGCGGCAAGC TCAACCACCA AGAATTCAAA TCCTGTTTAC GTGCCCTGGG TTACGATTTA 
CCAATGGTGG AAGAAGGCCA GCCCGACCCG GAGTTCGAGG AAATTTTGAA TGTGGTGGAC 
CCGAACCGCG ATGGACATGT TTCACTCCAG GAATACATAG CCTTTATGAT CTCCAAGGAA 
ACGGAGAACG TACAAAGCTA CGAGGAAATC GAAAATGCTT TCCGCGCAAT CACCGCCTCC 
GATCGTCCTT ACGTCACCAA GGATGAACTT TATTCCAACC TCACCAAGGA CATGGCAGAT 
TACTGCGTAC AGCGGATGAA ACCGTTCAAC GACCCGAAAA CGGGTCATCC CATTACCGGT 
GCCCTCGATT ATGTGGAGTT CACGCGAACT CTATTCCAAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5566099 ( XM_021843517.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021699209.1 Aedes aegypti spectrin alpha chain (LOC5566099), transcript variant X1, mRNA.

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CloneID	OAe121121
Clone ID Related Accession (Same CDS sequence)	XM_021843517.1
Accession Version	XM_021843517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	spectrin alpha chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAGT TTACCCCCAA GGAGGTAAAA ATCCTCGAAA GCGCTGAGGA TATTCAAGAG 
CGCCGTGAAC AGGTGCTCAA CCGATACAAT GAGTTCAAGG TCGAAACGCG CCACAAGCGC 
GAGAAGCTGG AAGATTCGCG TCGTTTCCAG TACTTTAAGC GTGACTCGGA CGAGTTGGAA 
AGCTGGATCC ACGAGAAATT GCAGGCCGCT TCGGAGGAGA GCTATCGCGA TCCTACCAAT 
TTGCAGGCCA AGATTCAGAA GCATCAGGCG TTCGAGGCTG AAGTTTCGGC CCATAGCAAT 
GCGATCGTAG TATTGGACAA CACCGGTCAG GAGATGATCA ATCAGCACCA CTTTGCCTCG 
GACACTATCC AACGTCGCTT GGATGAGTTG CACCGTTTGT GGGAATTGTT GCTGTCCCGT 
TTGGCCGAAA AGGGAATGAA ATTGCAACAG GCTCTGGTCT TGGTGCAATT CTTGCGCCAC 
TGCGAGGAGG TCATGTTCTG GATCAAGGAC AAGGAAGCTT TTGTGACGGC CGATGAGTTC 
GGTCAAGATT TGGAACATGT CGAAGTCTTG CAACGTAAGT TTGACGAGTT CCAGAAGGAC 
ATGGCTTCGC AAGAGTATCG AGTTACTGAA GTCAACGAGT TGGCTGACAA GTTGCTCTCA 
AACGGACACC CTGAGCGTGA AACTATCACT CGCAAGAAGG AAGAATTGAA CGAGGCTTGG 
CAACGCCTGA AGCAGCTGGC TATTCTGCGT CAGGAAAAGC TTTTCGGTGC CCATGAAATT 
CAACGTTTCA ATCGCGATGC TGATGAAACC GTCGCTTGGA TTGCTGAAAA AGACGTTGTT 
CTGTCGTCGG ATGATTATGG ACGTGACTTG GCTAGTGTTC AAGCTTTGCA ACGCAAGCAC 
GAAGGGGTAG AGCGCGACTT GGCTGCTCTG GAAGATAAGG TGGCCACTTT GGGAGCTGAA 
GCCGGTCGCT TATGTAGCAT CCACGCCGAT CATAGTGATC AAATTCGTGA GAAGCAAGCT 
GAAATTGCTG CCTACTGGCA ATCATTGACT GCCAAGGCCA AGGAACGTAA GCAAAAACTG 
GATGAATCAT ATTTCTTGCA CCGTTTCTTG GCTGATTTCC GCGATTTGGT TTCGTGGATC 
AACGGAATGA AGGCCATTAT CTCGGCTGAT GAACTTGCAA AGGATGTAGC TGGAGCTGAG 
GCTCTGCTTG AGCGTCACCA GGAGCACAAA GGAGAAATCG ATGCCCGTGG AGATAGCTTC 
AAGGTTACAA CCGAGGCTGG TCGTCAGCTT TTGGAACGGG AGCATTATGC CGCTGCTGAA 
GTGCAGGAAA AATTGGCTGC TCTAGAGAGC GATAAGAGTT CGCTTCTCGC GCTTTGGGAA 
GAACGTCGCA TTTTGTATGA ACAGTGCATG GATCTACAAC TGTTCTATCG TGACACTGAG 
CAGGCAGATA CTTGGATGGC CAAACAGGAA GCTTTCCTAG CCAATGAAGA TCTAGGAGAT 
TCGCTTGATT CCGTCGAAGC GCTGATTAAG AAGCACGAAG ATTTCGAGAA AAGTTTGGCG 
GCCCAAGAGG AGAAGATCAA GGCTTTGGAT GTTTTCGCTA CAAAGCTCAT CGATGGTCAG 
CATTACGCTG CTGACGATGT TGCCCAACGT CGTTCAATGT TGTTAGCTCG TCGATCAGCC 
TTGCTCGAGA AGTCTTCTGT ACGCCGTCAA CTGCTGGAGG ATTCCAGTGC TTTGCAGCAA 
TTTGAACGTG ACTGCGATGA GACTAAAGGA TGGATCAGCG AAAAGCTTAA ATTTGCCACA 
GATGACAGCT ATTTGGACCC AACCAATCTG AATGGAAAAG TTCAGAAACA TACCAACTTT 
GAGCATGAAT TAACGGCCAA CAAGAGCCGC ATTGAAGACA TCACCACCAA TGGTCAGAAT 
TTAGTCGAGA AGGGACACTA CGGTGCCGAT CAAATCAACT CGCGCATGCA GGAGATTGTC 
ACTTTGTGGG AATCTTTGGT TCAATCATCG GACAAAAAGG GCTGCAAATT GCAGGAAGCA 
TCCCAACAGC AACAATTCAA TCGCACCGTT GAAGATATTG AACTGTGGTT GAGCGAAGTG 
GAAGGCCAAC TAATGTCCGA GGATTATGGC AAAGACCTGA CCAGTGTCCA GAATTTGCAG 
AAGAAACATG GTTTGTTGGA GGCTGATGTC ATGGCCCATC AAGATCGCAT TGAAGGAATT 
AAAGTTGCAG CGAACAAGTT CGTCGAGAGT GGTCATTTCG ATGCCGACAA CATCCGTAAT 
AAGGAATCTG CGCTGTCCAA GCGTTATGGA GCTCTGGCTA CTCCGATGGC TGAACGAAAG 
CAGCGTTTGT TGGATTCCCT ACAGGTGCAG CAACTGTTCC GTGATCTGGA AGACGAAGCT 
GCATGGATCC GTGAGAAGGA ACCTGTCGCT GCATCCACCA ACCGTGGCCG CGATTTAATC 
GGTGTTCAGA ATTTGATTAA GAAGCATCAG GCCGTGTTGG CAGAAATCAA CAATCATGAA 
AATCGCGTCG CCGGAGTCAT CGCGAATGGC GAGCAAATGT TGACTGAGCA GCCTTTTGCC 
ACCGAAGATA TCAAATTGCG TTTGGATGCT GTTAAAGATC AATGGAATTC ATTGAAGGAG 
AAGGCTAATC AACGGAAGCA AGATCTGGAA GATTCGTTGC AGGCCCACCA GTACTTTGCT 
GATGCTAACG AGGCGGAATC TTGGATGCGT GAGAAGGAAC CTATCGTATC GAATCAAGAT 
TACGGCAAGG ACGAAGATTC GTCTGAAGCT TTGTTGAAGA AGCATGAAGC ATTGGTGTCC 
GATTTGGAAG CTTTTGGTAA CACCATTCAA GCTTTGCAAG AACAGGCCAA GAATTGTCGT 
CAGCAGGAAA CACCAGTGGT GGACATTACC GGAAAGGAAT GCGTCATGGC TCTGTATGAT 
TATACCGAGA AATCTCCTCG TGAAGTTTCG ATGAAGAAGG GGGACGTATT AACTCTTTTG 
AACTCGAACA ACAAGGATTG GTGGAAGGTA GAGGTCAACG ATCGTCAGGG ATTCGTTCCG 
GCTGCATACA TTAAGAAGAT CGATCCTGGC CTAAGCGCCA GTCAACAGAA TTTGGTTGAT 
GGCCATTCAA TTGCTAAGCG CCAGGCTCAA ATCAACAGCC AGTACGATAA CTTGCTTGCT 
TTGGCACGTG AACGACAGAA CAAATTGAAT GAAACCGTAA AGGCTTATGT TCTGGTACGT 
GAAGCTGCTG ATTTGTCCGC TTGGATTCGC GACAAGGAAA GCCACGCTCA GATTAAGGAT 
GTTGGCGAAG ATCTGGAAGA AGTTGAAGTT ATGCAGAAGA AGTTTGATGA CTTCAATGAT 
GATCTGAAGG CTAACGAAGT TCGTCTAGCT AAGTTGAACG AGATTGCCGT ACAGCTGACT 
TCGCTAGGTC AAACCGAAGC AGCATTGAAG ATCAAAACTC AAATTCAGAC CTTGAACGAA 
GAGTGGACAG TTTTGCAGAA TATTACCCAG GAAAGGGCTA GTCAACTTGG ATCGGCTCAT 
GAGGTTCAAC GCTTCCATCG TGATGTAGAT GAAACCAAGG ATTGGATTGG CGAGAAGGAC 
ACAGCTTTGA CGAATGACGA TCTTGGTAAG GATCTGCGTG GCGTTCAGAC CCTGCAACGC 
AAGCATGAAG GTTTGGAGCG TGATTTGGCA GCTCTACGAG ACAAAATTCG TCAATTGGAC 
GAAACTGCCA ATCGCCTCAT GCAATCCCAC CCGGAAACCG CCGAGCAGAC TTATGCCAAG 
CAGAAGGAAA TCAACGAGGA ATGGCAGCAG GTTGTTGCCA AAACTCAGCA ACGCAAGGAG 
AAGCTGTTAG ATTCTTATGA TCTACAGCGG TTCCTGAGCG ATTATCGCGA TCTGATGGCT 
TGGATCAGCT CAATGATGGG CTTGGTCACG TCCGAAGAGT TGGCCAACGA TGTCACCGGG 
GCTGAAGCTT TAATCGAAAG ACATCAGAAT CATCGTGCTG AAATTGATTT TCGTTACGGT 
ATCCCGCAGG AACACCGTAC CGAGGTAGAT GCCCGCGCTG GAACCTTCGC TGCGTTCGAT 
CAGTTTGGAG CTGAGCTGTT GCAGGCAAAT CATTATGCGT CACCCGAAAT TCAAGAGAAA 
ATTGAAAACT TGGCCAAGGC ACGTGAGGAT TTGGAGCGTG CCTGGACTGC TCGTCGTCTG 
CAGTTGGACC AGAATCTGGA TCTTCAGCTG TATCTGCGTG ATTGCGAACA GGCTGAGAAC 
TGGATGAGCG CCCGTGAAGC ATTCCTGAAT GCCGAAGAAG TCGACTCCAA GGGCGACAAC 
GTGGAAGCAT TGATTAAGAA GCACGAAGAC TTTGACAAAG CTATCAACGG TCATGAGGAG 
AAAATTGCCG CCCTGCAGGT GCTGGCTGAT CAATTGATTG CCCAGGAGCA CTACGCAGGA 
AAATTAATCG ACGACAAGCG TGCAGATGTG TTGGATCGTT GGCGTCACTT GAAGGAAGAT 
TTGATTGAAA AACGATCTCG CTTGGGAGAT GAGCAAACTC TACAGCAATT CTCACGTGAT 
GCTGATGAAA TCGAAAACTG GATTGCCGAG AAGCTGCAAC TAGCCACGGA GGAAAGTTAC 
AAGGACCCGG CCAATATTCA ATCCAAGCAT CAGAAGCACC AGGCTTTCGA AGCCGAATTG 
GCTGCTAATG CCGATCGTAT TCAGAGCGTG TTGGCGATGG GAAGCAATTT GATCGATAAG 
AATCAATGCA GTGGGTCCGA AGATGCAGTT CAGAAGCGTT TGACACAGAT CGCTGATCAG 
TGGGAATATC TGACCCAGAA AACAACGGAG AAATCCCTGA AGCTGAAGGA AGCTAACAAA 
CAGCGCACTT ATATTGCTGC TGTCAAGGAT TTGGACTTCT GGTTGGGTGA AGTTGAGAGT 
CTTTTGACCT CAGAAGATGC TGGAAAAGAT TTGGCTTCCG TCCAAAATCT TATGAAGAAG 
CACCAGCTGG TAGAGGCTGA CATCCATGCC CATGAAGATC GCATCAAGGA TATGAACGCC 
CAAGCCGACT CATTGGTCGA AAGTGGCCAA TTTGACAGTG CAGGAATTCA AGAAAAGCGT 
CAGTCCATCA ACGAGCGTTA CGAACGTATT TGTAATTTGG CAGCACATCG CCAAGCTCGC 
TTGAACGAAG CCAACACTTT GCATCAATTC TTCCGTGACA TTGCCGACGA GGAATCCTGG 
ATTAAGGAAA AGAAATTGTT GGTTGGTTCG GATGATTATG GTCGCGATTT AACCGGTGTC 
CAAAATCTGA AAAAGAAGCA CAAGCGTCTT GAGGCTGAGT TGGCTTCTCA CGAGCCAGCA 
ATTCAGGCTG TTCAGGAAGC TGGTGAAAAG TTGATGGATG TTTCGAATCT TGGTGTTCCA 
GAAATTGAGC AACGTCTGAA GGCTCTGAAC CAAGCCTGGG CTGAATTGAA GAGCTTGGCT 
GCTACTCGTG GTCAGAAACT GGATGAATCT CTCATTTATC AACAATTCTT AGCTAAGGTT 
GAAGAAGAGG AAGCATGGAT TACCGAGAAG CAACAACTGT TGTCGGTTGA AGATTATGGA 
GATTCGATGG CTGCTGTCCA GGGTCTTTTG AAGAAACATG ACGCCTTCGA GACCGATTTC 
GCCGCTCATC GTGATCGTTG CGCTGACATT CATGACAATG GTCAAACTTT GGTGACCAAT 
AATAATCATC ACGCCGAAAG CATTTCGCAG CGCTGCACAC AACTGGATAA GAAGTTGGAG 
AATCTGCAGG CGTTGGCTAC TCGTCGCAAG AGCGCCCTTC TGGACAACTT TGCTTATCTT 
CAATTCATGT GGAAGGCCGA TGTGGTTGAA AGTTGGATTG CCGATAAAGA AAATCACGTC 
AAATCGGAAG AGTTTGGACG TGATTTGTCT ACTGTTCAAA CATTGCTAAC CAAACAGGAG 
ACATTCGACG CTGGTCTCTC TGCATTCGAA CATGAAGGAA TCCATAATAT CACTGCGTTG 
AAAGATCAAC TAATTAACGC CAATCACGCC CAATCGGCCG CCATCCTCAA GCGCCACGAA 
GATGTGTTGA CCCGCTGGCA GAAATTGCGC GCTGATTCGG AGGCTCGCAA GTATCGTCTA 
TTGGCCATGC AGGAGCAGTT CCGTCAGATT GAAGATCTCT ATCTGACGTT TGCCAAGAAG 
GCTTCCGCTT TCAACTCGTG GTTCGAAAAC GCTGAAGAAG ATCTCACCGA TCCGGTGCGG 
TGCAATTCGA TTGAGGAAAT CAAGGCGCTG CGCGAAGCTC ACGCCCAGTT CCAGGCATCG 
TTGTCATCAG CCCAGTATGA CTTCCAAGCA TTGGCCGATC TAGACCGTAA AATCAAGAGC 
TTCAACGTTG GTCCCAATCC GTACACGTGG TTCACAATGG AAGCTCTAGA AGACACCTGG 
CGTAATCTGC AAAAGATCAT CGAAGAACGT GATGCTGAAT TGGCAAAGGA AGTTCACCGT 
CAAGAAGAGA ACGACAAGCT TCGCAAGGAA TTCGCCAAAC ATGCCAACTT GTTCCACCAG 
TGGCTTACCG AAACAAGATA CAGCATTCTG GGATGGGACA AAAATGGAAC ATCTCTTATG 
GACGGATCCG GTTCGTTGGA GGAACAATTT GAAGCGCTTT GTCACAAAGC AAATGAAATT 
CGCGCTCGTC GTGGAGATCT GAAGAAGATT GAAGAGTTGG GTGCCACCTT GGAAGAGCAC 
CTTATTTTGG ATAACCGCTA TACTGAACAC TCAACTGTAG GCCTTGCACA ACAGTGGGAT 
CAGCTTGACC AACTTGCCAT GCGCATGCAG CACAACCTGA GACAACAAAT CCAAGCCCGC 
AATCAATCTG GCGTCTCGGA GGATTCACTC AAAGAATTCT CAATGATGTT CAAGCACTTC 
GACAAGGACA AGAGCGGCAA GCTCAACCAC CAAGAATTCA AATCCTGTTT ACGTGCCCTG 
GGTTACGATT TACCAATGGT GGAGGAAGGC CAGCCCGACC CGGAGTTCGA GGAAATTTTG 
AATGTGGTGG ACCCGAACCG CGATGGACAT GTTTCACTCC AGGAATACAT AGCCTTTATG 
ATCTCCAAGG AAACGGAGAA CGTACAAAGC TACGAGGAAA TCGAAAATGC TTTCCGCGCA 
ATCACCGCCT CCGATCGTCC TTACGTCACC AAGGATGAAC TTTATTCCAA CCTCACCAAG 
GACATGGCAG ATTACTGCGT ACAGCGGATG AAACCGTTCA ACGACCCGAA AACGGGCCAT 
CCCATCACCG GTGCCCTCGA TTATGTGGAG TTCACGCGAA CTCTATTCCA AAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021699209.1
CDS	273..7589
Translation	MEQFTPKEVKILESAEDIQERREQVLNRYNEFKVETRHKREKLEDSRRFQYFKRDSDELESWIHEKLQAASEESYRDPTNLQAKIQKHQAFEAEVSAHSNAIVVLDNTGQEMINQHHFASDTIQRRLDELHRLWELLLSRLAEKGMKLQQALVLVQFLRHCEEVMFWIKDKEAFVTADEFGQDLEHVEVLQRKFDEFQKDMASQEYRVTEVNELADKLLSNGHPERETITRKKEELNEAWQRLKQLAILRQEKLFGAHEIQRFNRDADETVAWIAEKDVVLSSDDYGRDLASVQALQRKHEGVERDLAALEDKVATLGAEAGRLCSIHADHSDQIREKQAEIAAYWQSLTAKAKERKQKLDESYFLHRFLADFRDLVSWINGMKAIISADELAKDVAGAEALLERHQEHKGEIDARGDSFKVTTEAGRQLLEREHYAAAEVQEKLAALESDKSSLLALWEERRILYEQCMDLQLFYRDTEQADTWMAKQEAFLANEDLGDSLDSVEALIKKHEDFEKSLAAQEEKIKALDVFATKLIDGQHYAADDVAQRRSMLLARRSALLEKSSVRRQLLEDSSALQQFERDCDETKGWISEKLKFATDDSYLDPTNLNGKVQKHTNFEHELTANKSRIEDITTNGQNLVEKGHYGADQINSRMQEIVTLWESLVQSSDKKGCKLQEASQQQQFNRTVEDIELWLSEVEGQLMSEDYGKDLTSVQNLQKKHGLLEADVMAHQDRIEGIKVAANKFVESGHFDADNIRNKESALSKRYGALATPMAERKQRLLDSLQVQQLFRDLEDEAAWIREKEPVAASTNRGRDLIGVQNLIKKHQAVLAEINNHENRVAGVIANGEQMLTEQPFATEDIKLRLDAVKDQWNSLKEKANQRKQDLEDSLQAHQYFADANEAESWMREKEPIVSNQDYGKDEDSSEALLKKHEALVSDLEAFGNTIQALQEQAKNCRQQETPVVDITGKECVMALYDYTEKSPREVSMKKGDVLTLLNSNNKDWWKVEVNDRQGFVPAAYIKKIDPGLSASQQNLVDGHSIAKRQAQINSQYDNLLALARERQNKLNETVKAYVLVREAADLSAWIRDKESHAQIKDVGEDLEEVEVMQKKFDDFNDDLKANEVRLAKLNEIAVQLTSLGQTEAALKIKTQIQTLNEEWTVLQNITQERASQLGSAHEVQRFHRDVDETKDWIGEKDTALTNDDLGKDLRGVQTLQRKHEGLERDLAALRDKIRQLDETANRLMQSHPETAEQTYAKQKEINEEWQQVVAKTQQRKEKLLDSYDLQRFLSDYRDLMAWISSMMGLVTSEELANDVTGAEALIERHQNHRAEIDFRYGIPQEHRTEVDARAGTFAAFDQFGAELLQANHYASPEIQEKIENLAKAREDLERAWTARRLQLDQNLDLQLYLRDCEQAENWMSAREAFLNAEEVDSKGDNVEALIKKHEDFDKAINGHEEKIAALQVLADQLIAQEHYAGKLIDDKRADVLDRWRHLKEDLIEKRSRLGDEQTLQQFSRDADEIENWIAEKLQLATEESYKDPANIQSKHQKHQAFEAELAANADRIQSVLAMGSNLIDKNQCSGSEDAVQKRLTQIADQWEYLTQKTTEKSLKLKEANKQRTYIAAVKDLDFWLGEVESLLTSEDAGKDLASVQNLMKKHQLVEADIHAHEDRIKDMNAQADSLVESGQFDSAGIQEKRQSINERYERICNLAAHRQARLNEANTLHQFFRDIADEESWIKEKKLLVGSDDYGRDLTGVQNLKKKHKRLEAELASHEPAIQAVQEAGEKLMDVSNLGVPEIEQRLKALNQAWAELKSLAATRGQKLDESLIYQQFLAKVEEEEAWITEKQQLLSVEDYGDSMAAVQGLLKKHDAFETDFAAHRDRCADIHDNGQTLVTNNNHHAESISQRCTQLDKKLENLQALATRRKSALLDNFAYLQFMWKADVVESWIADKENHVKSEEFGRDLSTVQTLLTKQETFDAGLSAFEHEGIHNITALKDQLINANHAQSAAILKRHEDVLTRWQKLRADSEARKYRLLAMQEQFRQIEDLYLTFAKKASAFNSWFENAEEDLTDPVRCNSIEEIKALREAHAQFQASLSSAQYDFQALADLDRKIKSFNVGPNPYTWFTMEALEDTWRNLQKIIEERDAELAKEVHRQEENDKLRKEFAKHANLFHQWLTETRYSILGWDKNGTSLMDGSGSLEEQFEALCHKANEIRARRGDLKKIEELGATLEEHLILDNRYTEHSTVGLAQQWDQLDQLAMRMQHNLRQQIQARNQSGVSEDSLKEFSMMFKHFDKDKSGKLNHQEFKSCLRALGYDLPMVEEGQPDPEFEEILNVVDPNRDGHVSLQEYIAFMISKETENVQSYEEIENAFRAITASDRPYVTKDELYSNLTKDMADYCVQRMKPFNDPKTGHPITGALDYVEFTRTLFQN

Target ORF information:

RefSeq Version	XM_021843517.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti spectrin alpha chain (LOC5566099), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021843517.1

ORF Insert Sequence:

ATGGAACAGT TTACCCCCAA GGAGGTAAAA ATCCTCGAAA GCGCTGAGGA TATTCAAGAG 
CGCCGTGAAC AGGTGCTCAA CCGATACAAT GAGTTCAAGG TCGAAACGCG CCACAAGCGC 
GAGAAGCTGG AAGATTCGCG TCGTTTCCAG TACTTTAAGC GTGACTCGGA CGAGTTGGAA 
AGCTGGATCC ACGAGAAATT GCAGGCCGCT TCGGAGGAGA GCTATCGCGA TCCTACCAAT 
TTGCAGGCCA AGATTCAGAA GCATCAGGCG TTCGAGGCTG AAGTTTCGGC CCATAGCAAT 
GCGATCGTAG TATTGGACAA CACCGGTCAG GAGATGATCA ATCAGCACCA CTTTGCCTCG 
GACACTATCC AACGTCGCTT GGATGAGTTG CACCGTTTGT GGGAATTGTT GCTGTCCCGT 
TTGGCCGAAA AGGGAATGAA ATTGCAACAG GCTCTGGTCT TGGTGCAATT CTTGCGCCAC 
TGCGAGGAGG TCATGTTCTG GATCAAGGAC AAGGAAGCTT TTGTGACGGC CGATGAGTTC 
GGTCAAGATT TGGAACATGT CGAAGTCTTG CAACGTAAGT TTGACGAGTT CCAGAAGGAC 
ATGGCTTCGC AAGAGTATCG AGTTACTGAA GTCAACGAGT TGGCTGACAA GTTGCTCTCA 
AACGGACACC CTGAGCGTGA AACTATCACT CGCAAGAAGG AAGAATTGAA CGAGGCTTGG 
CAACGCCTGA AGCAGCTGGC TATTCTGCGT CAGGAAAAGC TTTTCGGTGC CCATGAAATT 
CAACGTTTCA ATCGCGATGC TGATGAAACC GTCGCTTGGA TTGCTGAAAA AGACGTTGTT 
CTGTCGTCGG ATGATTATGG ACGTGACTTG GCTAGTGTTC AAGCTTTGCA ACGCAAGCAC 
GAAGGGGTAG AGCGCGACTT GGCTGCTCTG GAAGATAAGG TGGCCACTTT GGGAGCTGAA 
GCCGGTCGCT TATGTAGCAT CCACGCCGAT CATAGTGATC AAATTCGTGA GAAGCAAGCT 
GAAATTGCTG CCTACTGGCA ATCATTGACT GCCAAGGCCA AGGAACGTAA GCAAAAACTG 
GATGAATCAT ATTTCTTGCA CCGTTTCTTG GCTGATTTCC GCGATTTGGT TTCGTGGATC 
AACGGAATGA AGGCCATTAT CTCGGCTGAT GAACTTGCAA AGGATGTAGC TGGAGCTGAG 
GCTCTGCTTG AGCGTCACCA GGAGCACAAA GGAGAAATCG ATGCCCGTGG AGATAGCTTC 
AAGGTTACAA CCGAGGCTGG TCGTCAGCTT TTGGAACGGG AGCATTATGC CGCTGCTGAA 
GTGCAGGAAA AATTGGCTGC TCTAGAGAGC GATAAGAGTT CGCTTCTCGC GCTTTGGGAA 
GAACGTCGCA TTTTGTATGA ACAGTGCATG GATCTACAAC TGTTCTATCG TGACACTGAG 
CAGGCAGATA CTTGGATGGC CAAACAGGAA GCTTTCCTAG CCAATGAAGA TCTAGGAGAT 
TCGCTTGATT CCGTCGAAGC GCTGATTAAG AAGCACGAAG ATTTCGAGAA AAGTTTGGCG 
GCCCAAGAGG AGAAGATCAA GGCTTTGGAT GTTTTCGCTA CAAAGCTCAT CGATGGTCAG 
CATTACGCTG CTGACGATGT TGCCCAACGT CGTTCAATGT TGTTAGCTCG TCGATCAGCC 
TTGCTCGAGA AGTCTTCTGT ACGCCGTCAA CTGCTGGAGG ATTCCAGTGC TTTGCAGCAA 
TTTGAACGTG ACTGCGATGA GACTAAAGGA TGGATCAGCG AAAAGCTTAA ATTTGCCACA 
GATGACAGCT ATTTGGACCC AACCAATCTG AATGGAAAAG TTCAGAAACA TACCAACTTT 
GAGCATGAAT TAACGGCCAA CAAGAGCCGC ATTGAAGACA TCACCACCAA TGGTCAGAAT 
TTAGTCGAGA AGGGACACTA CGGTGCCGAT CAAATCAACT CGCGCATGCA GGAGATTGTC 
ACTTTGTGGG AATCTTTGGT TCAATCATCG GACAAAAAGG GCTGCAAATT GCAGGAAGCA 
TCCCAACAGC AACAATTCAA TCGCACCGTT GAAGATATTG AACTGTGGTT GAGCGAAGTG 
GAAGGCCAAC TAATGTCCGA GGATTATGGC AAAGACCTGA CCAGTGTCCA GAATTTGCAG 
AAGAAACATG GTTTGTTGGA GGCTGATGTC ATGGCCCATC AAGATCGCAT TGAAGGAATT 
AAAGTTGCAG CGAACAAGTT CGTCGAGAGT GGTCATTTCG ATGCCGACAA CATCCGTAAT 
AAGGAATCTG CGCTGTCCAA GCGTTATGGA GCTCTGGCTA CTCCGATGGC TGAACGAAAG 
CAGCGTTTGT TGGATTCCCT ACAGGTGCAG CAACTGTTCC GTGATCTGGA AGACGAAGCT 
GCATGGATCC GTGAGAAGGA ACCTGTCGCT GCATCCACCA ACCGTGGCCG CGATTTAATC 
GGTGTTCAGA ATTTGATTAA GAAGCATCAG GCCGTGTTGG CAGAAATCAA CAATCATGAA 
AATCGCGTCG CCGGAGTCAT CGCGAATGGC GAGCAAATGT TGACTGAGCA GCCTTTTGCC 
ACCGAAGATA TCAAATTGCG TTTGGATGCT GTTAAAGATC AATGGAATTC ATTGAAGGAG 
AAGGCTAATC AACGGAAGCA AGATCTGGAA GATTCGTTGC AGGCCCACCA GTACTTTGCT 
GATGCTAACG AGGCGGAATC TTGGATGCGT GAGAAGGAAC CTATCGTATC GAATCAAGAT 
TACGGCAAGG ACGAAGATTC GTCTGAAGCT TTGTTGAAGA AGCATGAAGC ATTGGTGTCC 
GATTTGGAAG CTTTTGGTAA CACCATTCAA GCTTTGCAAG AACAGGCCAA GAATTGTCGT 
CAGCAGGAAA CACCAGTGGT GGACATTACC GGAAAGGAAT GCGTCATGGC TCTGTATGAT 
TATACCGAGA AATCTCCTCG TGAAGTTTCG ATGAAGAAGG GGGACGTATT AACTCTTTTG 
AACTCGAACA ACAAGGATTG GTGGAAGGTA GAGGTCAACG ATCGTCAGGG ATTCGTTCCG 
GCTGCATACA TTAAGAAGAT CGATCCTGGC CTAAGCGCCA GTCAACAGAA TTTGGTTGAT 
GGCCATTCAA TTGCTAAGCG CCAGGCTCAA ATCAACAGCC AGTACGATAA CTTGCTTGCT 
TTGGCACGTG AACGACAGAA CAAATTGAAT GAAACCGTAA AGGCTTATGT TCTGGTACGT 
GAAGCTGCTG ATTTGTCCGC TTGGATTCGC GACAAGGAAA GCCACGCTCA GATTAAGGAT 
GTTGGCGAAG ATCTGGAAGA AGTTGAAGTT ATGCAGAAGA AGTTTGATGA CTTCAATGAT 
GATCTGAAGG CTAACGAAGT TCGTCTAGCT AAGTTGAACG AGATTGCCGT ACAGCTGACT 
TCGCTAGGTC AAACCGAAGC AGCATTGAAG ATCAAAACTC AAATTCAGAC CTTGAACGAA 
GAGTGGACAG TTTTGCAGAA TATTACCCAG GAAAGGGCTA GTCAACTTGG ATCGGCTCAT 
GAGGTTCAAC GCTTCCATCG TGATGTAGAT GAAACCAAGG ATTGGATTGG CGAGAAGGAC 
ACAGCTTTGA CGAATGACGA TCTTGGTAAG GATCTGCGTG GCGTTCAGAC CCTGCAACGC 
AAGCATGAAG GTTTGGAGCG TGATTTGGCA GCTCTACGAG ACAAAATTCG TCAATTGGAC 
GAAACTGCCA ATCGCCTCAT GCAATCCCAC CCGGAAACCG CCGAGCAGAC TTATGCCAAG 
CAGAAGGAAA TCAACGAGGA ATGGCAGCAG GTTGTTGCCA AAACTCAGCA ACGCAAGGAG 
AAGCTGTTAG ATTCTTATGA TCTACAGCGG TTCCTGAGCG ATTATCGCGA TCTGATGGCT 
TGGATCAGCT CAATGATGGG CTTGGTCACG TCCGAAGAGT TGGCCAACGA TGTCACCGGG 
GCTGAAGCTT TAATCGAAAG ACATCAGAAT CATCGTGCTG AAATTGATTT TCGTTACGGT 
ATCCCGCAGG AACACCGTAC CGAGGTAGAT GCCCGCGCTG GAACCTTCGC TGCGTTCGAT 
CAGTTTGGAG CTGAGCTGTT GCAGGCAAAT CATTATGCGT CACCCGAAAT TCAAGAGAAA 
ATTGAAAACT TGGCCAAGGC ACGTGAGGAT TTGGAGCGTG CCTGGACTGC TCGTCGTCTG 
CAGTTGGACC AGAATCTGGA TCTTCAGCTG TATCTGCGTG ATTGCGAACA GGCTGAGAAC 
TGGATGAGCG CCCGTGAAGC ATTCCTGAAT GCCGAAGAAG TCGACTCCAA GGGCGACAAC 
GTGGAAGCAT TGATTAAGAA GCACGAAGAC TTTGACAAAG CTATCAACGG TCATGAGGAG 
AAAATTGCCG CCCTGCAGGT GCTGGCTGAT CAATTGATTG CCCAGGAGCA CTACGCAGGA 
AAATTAATCG ACGACAAGCG TGCAGATGTG TTGGATCGTT GGCGTCACTT GAAGGAAGAT 
TTGATTGAAA AACGATCTCG CTTGGGAGAT GAGCAAACTC TACAGCAATT CTCACGTGAT 
GCTGATGAAA TCGAAAACTG GATTGCCGAG AAGCTGCAAC TAGCCACGGA GGAAAGTTAC 
AAGGACCCGG CCAATATTCA ATCCAAGCAT CAGAAGCACC AGGCTTTCGA AGCCGAATTG 
GCTGCTAATG CCGATCGTAT TCAGAGCGTG TTGGCGATGG GAAGCAATTT GATCGATAAG 
AATCAATGCA GTGGGTCCGA AGATGCAGTT CAGAAGCGTT TGACACAGAT CGCTGATCAG 
TGGGAATATC TGACCCAGAA AACAACGGAG AAATCCCTGA AGCTGAAGGA AGCTAACAAA 
CAGCGCACTT ATATTGCTGC TGTCAAGGAT TTGGACTTCT GGTTGGGTGA AGTTGAGAGT 
CTTTTGACCT CAGAAGATGC TGGAAAAGAT TTGGCTTCCG TCCAAAATCT TATGAAGAAG 
CACCAGCTGG TAGAGGCTGA CATCCATGCC CATGAAGATC GCATCAAGGA TATGAACGCC 
CAAGCCGACT CATTGGTCGA AAGTGGCCAA TTTGACAGTG CAGGAATTCA AGAAAAGCGT 
CAGTCCATCA ACGAGCGTTA CGAACGTATT TGTAATTTGG CAGCACATCG CCAAGCTCGC 
TTGAACGAAG CCAACACTTT GCATCAATTC TTCCGTGACA TTGCCGACGA GGAATCCTGG 
ATTAAGGAAA AGAAATTGTT GGTTGGTTCG GATGATTATG GTCGCGATTT AACCGGTGTC 
CAAAATCTGA AAAAGAAGCA CAAGCGTCTT GAGGCTGAGT TGGCTTCTCA CGAGCCAGCA 
ATTCAGGCTG TTCAGGAAGC TGGTGAAAAG TTGATGGATG TTTCGAATCT TGGTGTTCCA 
GAAATTGAGC AACGTCTGAA GGCTCTGAAC CAAGCCTGGG CTGAATTGAA GAGCTTGGCT 
GCTACTCGTG GTCAGAAACT GGATGAATCT CTCATTTATC AACAATTCTT AGCTAAGGTT 
GAAGAAGAGG AAGCATGGAT TACCGAGAAG CAACAACTGT TGTCGGTTGA AGATTATGGA 
GATTCGATGG CTGCTGTCCA GGGTCTTTTG AAGAAACATG ACGCCTTCGA GACCGATTTC 
GCCGCTCATC GTGATCGTTG CGCTGACATT CATGACAATG GTCAAACTTT GGTGACCAAT 
AATAATCATC ACGCCGAAAG CATTTCGCAG CGCTGCACAC AACTGGATAA GAAGTTGGAG 
AATCTGCAGG CGTTGGCTAC TCGTCGCAAG AGCGCCCTTC TGGACAACTT TGCTTATCTT 
CAATTCATGT GGAAGGCCGA TGTGGTTGAA AGTTGGATTG CCGATAAAGA AAATCACGTC 
AAATCGGAAG AGTTTGGACG TGATTTGTCT ACTGTTCAAA CATTGCTAAC CAAACAGGAG 
ACATTCGACG CTGGTCTCTC TGCATTCGAA CATGAAGGAA TCCATAATAT CACTGCGTTG 
AAAGATCAAC TAATTAACGC CAATCACGCC CAATCGGCCG CCATCCTCAA GCGCCACGAA 
GATGTGTTGA CCCGCTGGCA GAAATTGCGC GCTGATTCGG AGGCTCGCAA GTATCGTCTA 
TTGGCCATGC AGGAGCAGTT CCGTCAGATT GAAGATCTCT ATCTGACGTT TGCCAAGAAG 
GCTTCCGCTT TCAACTCGTG GTTCGAAAAC GCTGAAGAAG ATCTCACCGA TCCGGTGCGG 
TGCAATTCGA TTGAGGAAAT CAAGGCGCTG CGCGAAGCTC ACGCCCAGTT CCAGGCATCG 
TTGTCATCAG CCCAGTATGA CTTCCAAGCA TTGGCCGATC TAGACCGTAA AATCAAGAGC 
TTCAACGTTG GTCCCAATCC GTACACGTGG TTCACAATGG AAGCTCTAGA AGACACCTGG 
CGTAATCTGC AAAAGATCAT CGAAGAACGT GATGCTGAAT TGGCAAAGGA AGTTCACCGT 
CAAGAAGAGA ACGACAAGCT TCGCAAGGAA TTCGCCAAAC ATGCCAACTT GTTCCACCAG 
TGGCTTACCG AAACAAGATA CAGCATTCTG GGATGGGACA AAAATGGAAC ATCTCTTATG 
GACGGATCCG GTTCGTTGGA GGAACAATTT GAAGCGCTTT GTCACAAAGC AAATGAAATT 
CGCGCTCGTC GTGGAGATCT GAAGAAGATT GAAGAGTTGG GTGCCACCTT GGAAGAGCAC 
CTTATTTTGG ATAACCGCTA TACTGAACAC TCAACTGTAG GCCTTGCACA ACAGTGGGAT 
CAGCTTGACC AACTTGCCAT GCGCATGCAG CACAACCTGA GACAACAAAT CCAAGCCCGC 
AATCAATCTG GCGTCTCGGA GGATTCACTC AAAGAATTCT CAATGATGTT CAAGCACTTC 
GACAAGGACA AGAGCGGCAA GCTCAACCAC CAAGAATTCA AATCCTGTTT ACGTGCCCTG 
GGTTACGATT TACCAATGGT GGAGGAAGGC CAGCCCGACC CGGAGTTCGA GGAAATTTTG 
AATGTGGTGG ACCCGAACCG CGATGGACAT GTTTCACTCC AGGAATACAT AGCCTTTATG 
ATCTCCAAGG AAACGGAGAA CGTACAAAGC TACGAGGAAA TCGAAAATGC TTTCCGCGCA 
ATCACCGCCT CCGATCGTCC TTACGTCACC AAGGATGAAC TTTATTCCAA CCTCACCAAG 
GACATGGCAG ATTACTGCGT ACAGCGGATG AAACCGTTCA ACGACCCGAA AACGGGCCAT 
CCCATCACCG GTGCCCTCGA TTATGTGGAG TTCACGCGAA CTCTATTCCA AAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5566099 ( XM_021843518.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021699210.1 Aedes aegypti spectrin alpha chain (LOC5566099), transcript variant X2, mRNA.

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CloneID	OAe121122
Clone ID Related Accession (Same CDS sequence)	XM_021843518.1
Accession Version	XM_021843518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	spectrin alpha chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAGT TTACCCCCAA GGAGGTAAAA ATCCTCGAAA GCGCTGAGGA TATTCAAGAG 
CGCCGTGAAC AGGTGCTCAA CCGATACAAT GAGTTCAAGG TCGAAACGCG CCACAAGCGC 
GAGAAGCTGG AAGATTCGCG TCGTTTCCAG TACTTTAAGC GTGACTCGGA CGAGTTGGAA 
AGCTGGATCC ACGAGAAATT GCAGGCCGCT TCGGAGGAGA GCTATCGCGA TCCTACCAAT 
TTGCAGGCCA AGATTCAGAA GCATCAGGCG TTCGAGGCTG AAGTTTCGGC CCATAGCAAT 
GCGATCGTAG TATTGGACAA CACCGGTCAG GAGATGATCA ATCAGCACCA CTTTGCCTCG 
GACACTATCC AACGTCGCTT GGATGAGTTG CACCGTTTGT GGGAATTGTT GCTGTCCCGT 
TTGGCCGAAA AGGGAATGAA ATTGCAACAG GCTCTGGTCT TGGTGCAATT CTTGCGCCAC 
TGCGAGGAGG TCATGTTCTG GATCAAGGAC AAGGAAGCTT TTGTGACGGC CGATGAGTTC 
GGTCAAGATT TGGAACATGT CGAAGTCTTG CAACGTAAGT TTGACGAGTT CCAGAAGGAC 
ATGGCTTCGC AAGAGTATCG AGTTACTGAA GTCAACGAGT TGGCTGACAA GTTGCTCTCA 
AACGGACACC CTGAGCGTGA AACTATCACT CGCAAGAAGG AAGAATTGAA CGAGGCTTGG 
CAACGCCTGA AGCAGCTGGC TATTCTGCGT CAGGAAAAGC TTTTCGGTGC CCATGAAATT 
CAACGTTTCA ATCGCGATGC TGATGAAACC GTCGCTTGGA TTGCTGAAAA AGACGTTGTT 
CTGTCGTCGG ATGATTATGG ACGTGACTTG GCTAGTGTTC AAGCTTTGCA ACGCAAGCAC 
GAAGGGGTAG AGCGCGACTT GGCTGCTCTG GAAGATAAGG TGGCCACTTT GGGAGCTGAA 
GCCGGTCGCT TATGTAGCAT CCACGCCGAT CATAGTGATC AAATTCGTGA GAAGCAAGCT 
GAAATTGCTG CCTACTGGCA ATCATTGACT GCCAAGGCCA AGGAACGTAA GCAAAAACTG 
GATGAATCAT ATTTCTTGCA CCGTTTCTTG GCTGATTTCC GCGATTTGGT TTCGTGGATC 
AACGGAATGA AGGCCATTAT CTCGGCTGAT GAACTTGCAA AGGATGTAGC TGGAGCTGAG 
GCTCTGCTTG AGCGTCACCA GGAGCACAAA GGAGAAATCG ATGCCCGTGG AGATAGCTTC 
AAGGTTACAA CCGAGGCTGG TCGTCAGCTT TTGGAACGGG AGCATTATGC CGCTGCTGAA 
GTGCAGGAAA AATTGGCTGC TCTAGAGAGC GATAAGAGTT CGCTTCTCGC GCTTTGGGAA 
GAACGTCGCA TTTTGTATGA ACAGTGCATG GATCTACAAC TGTTCTATCG TGACACTGAG 
CAGGCAGATA CTTGGATGGC CAAACAGGAA GCTTTCCTAG CCAATGAAGA TCTAGGAGAT 
TCGCTTGATT CCGTCGAAGC GCTGATTAAG AAGCACGAAG ATTTCGAGAA AAGTTTGGCG 
GCCCAAGAGG AGAAGATCAA GGCTTTGGAT GTTTTCGCTA CAAAGCTCAT CGATGGTCAG 
CATTACGCTG CTGACGATGT TGCCCAACGT CGTTCAATGT TGTTAGCTCG TCGATCAGCC 
TTGCTCGAGA AGTCTTCTGT ACGCCGTCAA CTGCTGGAGG ATTCCAGTGC TTTGCAGCAA 
TTTGAACGTG ACTGCGATGA GACTAAAGGA TGGATCAGCG AAAAGCTTAA ATTTGCCACA 
GATGACAGCT ATTTGGACCC AACCAATCTG AATGGAAAAG TTCAGAAACA TACCAACTTT 
GAGCATGAAT TAACGGCCAA CAAGAGCCGC ATTGAAGACA TCACCACCAA TGGTCAGAAT 
TTAGTCGAGA AGGGACACTA CGGTGCCGAT CAAATCAACT CGCGCATGCA GGAGATTGTC 
ACTTTGTGGG AATCTTTGGT TCAATCATCG GACAAAAAGG GCTGCAAATT GCAGGAAGCA 
TCCCAACAGC AACAATTCAA TCGCACCGTT GAAGATATTG AACTGTGGTT GAGCGAAGTG 
GAAGGCCAAC TAATGTCCGA GGATTATGGC AAAGACCTGA CCAGTGTCCA GAATTTGCAG 
AAGAAACATG GTTTGTTGGA GGCTGATGTC ATGGCCCATC AAGATCGCAT TGAAGGAATT 
AAAGTTGCAG CGAACAAGTT CGTCGAGAGT GGTCATTTCG ATGCCGACAA CATCCGTAAT 
AAGGAATCTG CGCTGTCCAA GCGTTATGGA GCTCTGGCTA CTCCGATGGC TGAACGAAAG 
CAGCGTTTGT TGGATTCCCT ACAGGTGCAG CAACTGTTCC GTGATCTGGA AGACGAAGCT 
GCATGGATCC GTGAGAAGGA ACCTGTCGCT GCATCCACCA ACCGTGGCCG CGATTTAATC 
GGTGTTCAGA ATTTGATTAA GAAGCATCAG GCCGTGTTGG CAGAAATCAA CAATCATGAA 
AATCGCGTCG CCGGAGTCAT CGCGAATGGC GAGCAAATGT TGACTGAGCA GCCTTTTGCC 
ACCGAAGATA TCAAATTGCG TTTGGATGCT GTTAAAGATC AATGGAATTC ATTGAAGGAG 
AAGGCTAATC AACGGAAGCA AGATCTGGAA GATTCGTTGC AGGCCCACCA GTACTTTGCT 
GATGCTAACG AGGCGGAATC TTGGATGCGT GAGAAGGAAC CTATCGTATC GAATCAAGAT 
TACGGCAAGG ACGAAGATTC GTCTGAAGCT TTGTTGAAGA AGCATGAAGC ATTGGTGTCC 
GATTTGGAAG CTTTTGGTAA CACCATTCAA GCTTTGCAAG AACAGGCCAA GAATTGTCGT 
CAGCAGGAAA CACCAGTGGT GGACATTACC GGAAAGGAAT GCGTCATGGC TCTGTATGAT 
TATACCGAGA AATCTCCTCG TGAAGTTTCG ATGAAGAAGG GGGACGTATT AACTCTTTTG 
AACTCGAACA ACAAGGATTG GTGGAAGGTA GAGGTCAACG ATCGTCAGGG ATTCGTTCCG 
GCTGCATACA TTAAGAAGAT CGATCCTGGC CTAAGCGCCA GTCAACAGAA TTTGGTTGAT 
GGCCATTCAA TTGCTAAGCG CCAGGCTCAA ATCAACAGCC AGTACGATAA CTTGCTTGCT 
TTGGCACGTG AACGACAGAA CAAATTGAAT GAAACCGTAA AGGCTTATGT TCTGGTACGT 
GAAGCTGCTG ATTTGTCCGC TTGGATTCGC GACAAGGAAA GCCACGCTCA GATTAAGGAT 
GTTGGCGAAG ATCTGGAAGA AGTTGAAGTT ATGCAGAAGA AGTTTGATGA CTTCAATGAT 
GATCTGAAGG CTAACGAAGT TCGTCTAGCT AAGTTGAACG AGATTGCCGT ACAGCTGACT 
TCGCTAGGTC AAACCGAAGC AGCATTGAAG ATCAAAACTC AAATTCAGAC CTTGAACGAA 
GAGTGGACAG TTTTGCAGAA TATTACCCAG GAAAGGGCTA GTCAACTTGG ATCGGCTCAT 
GAGGTTCAAC GCTTCCATCG TGATGTAGAT GAAACCAAGG ATTGGATTGG CGAGAAGGAC 
ACAGCTTTGA CGAATGACGA TCTTGGTAAG GATCTGCGTG GCGTTCAGAC CCTGCAACGC 
AAGCATGAAG GTTTGGAGCG TGATTTGGCA GCTCTACGAG ACAAAATTCG TCAATTGGAC 
GAAACTGCCA ATCGCCTCAT GCAATCCCAC CCGGAAACCG CCGAGCAGAC TTATGCCAAG 
CAGAAGGAAA TCAACGAGGA ATGGCAGCAG GTTGTTGCCA AAACTCAGCA ACGCAAGGAG 
AAGCTGTTAG ATTCTTATGA TCTACAGCGG TTCCTGAGCG ATTATCGCGA TCTGATGGCT 
TGGATCAGCT CAATGATGGG CTTGGTCACG TCCGAAGAGT TGGCCAACGA TGTCACCGGG 
GCTGAAGCTT TAATCGAAAG ACATCAGAAT CATCGTGCTG AAATTGATTT TCGTTACGGT 
ATCCCGCAGG AACACCGTAC CGAGGTAGAT GCCCGCGCTG GAACCTTCGC TGCGTTCGAT 
CAGTTTGGAG CTGAGCTGTT GCAGGCAAAT CATTATGCGT CACCCGAAAT TCAAGAGAAA 
ATTGAAAACT TGGCCAAGGC ACGTGAGGAT TTGGAGCGTG CCTGGACTGC TCGTCGTCTG 
CAGTTGGACC AGAATCTGGA TCTTCAGCTG TATCTGCGTG ATTGCGAACA GGCTGAGAAC 
TGGATGAGCG CCCGTGAAGC ATTCCTGAAT GCCGAAGAAG TCGACTCCAA GGGCGACAAC 
GTGGAAGCAT TGATTAAGAA GCACGAAGAC TTTGACAAAG CTATCAACGG TCATGAGGAG 
AAAATTGCCG CCCTGCAGGT GCTGGCTGAT CAATTGATTG CCCAGGAGCA CTACGCAGGA 
AAATTAATCG ACGACAAGCG TGCAGATGTG TTGGATCGTT GGCGTCACTT GAAGGAAGAT 
TTGATTGAAA AACGATCTCG CTTGGGAGAT GAGCAAACTC TACAGCAATT CTCACGTGAT 
GCTGATGAAA TCGAAAACTG GATTGCCGAG AAGCTGCAAC TAGCCACGGA GGAAAGTTAC 
AAGGACCCGG CCAATATTCA ATCCAAGCAT CAGAAGCACC AGGCTTTCGA AGCCGAATTG 
GCTGCTAATG CCGATCGTAT TCAGAGCGTG TTGGCGATGG GAAGCAATTT GATCGATAAG 
AATCAATGCA GTGGGTCCGA AGATGCAGTT CAGAAGCGTT TGACACAGAT CGCTGATCAG 
TGGGAATATC TGACCCAGAA AACAACGGAG AAATCCCTGA AGCTGAAGGA AGCTAACAAA 
CAGCGCACTT ATATTGCTGC TGTCAAGGAT TTGGACTTCT GGTTGGGTGA AGTTGAGAGT 
CTTTTGACCT CAGAAGATGC TGGAAAAGAT TTGGCTTCCG TCCAAAATCT TATGAAGAAG 
CACCAGCTGG TAGAGGCTGA CATCCATGCC CATGAAGATC GCATCAAGGA TATGAACGCC 
CAAGCCGACT CATTGGTCGA AAGTGGCCAA TTTGACAGTG CAGGAATTCA AGAAAAGCGT 
CAGTCCATCA ACGAGCGTTA CGAACGTATT TGTAATTTGG CAGCACATCG CCAAGCTCGC 
TTGAACGAAG CCAACACTTT GCATCAATTC TTCCGTGACA TTGCCGACGA GGAATCCTGG 
ATTAAGGAAA AGAAATTGTT GGTTGGTTCG GATGATTATG GTCGCGATTT AACCGGTGTC 
CAAAATCTGA AAAAGAAGCA CAAGCGTCTT GAGGCTGAGT TGGCTTCTCA CGAGCCAGCA 
ATTCAGGCTG TTCAGGAAGC TGGTGAAAAG TTGATGGATG TTTCGAATCT TGGTGTTCCA 
GAAATTGAGC AACGTCTGAA GGCTCTGAAC CAAGCCTGGG CTGAATTGAA GAGCTTGGCT 
GCTACTCGTG GTCAGAAACT GGATGAATCT CTCATTTATC AACAATTCTT AGCTAAGGTT 
GAAGAAGAGG AAGCATGGAT TACCGAGAAG CAACAACTGT TGTCGGTTGA AGATTATGGA 
GATTCGATGG CTGCTGTCCA GGGTCTTTTG AAGAAACATG ACGCCTTCGA GACCGATTTC 
GCCGCTCATC GTGATCGTTG CGCTGACATT CATGACAATG GTCAAACTTT GGTGACCAAT 
AATAATCATC ACGCCGAAAG CATTTCGCAG CGCTGCACAC AACTGGATAA GAAGTTGGAG 
AATCTGCAGG CGTTGGCTAC TCGTCGCAAG AGCGCCCTTC TGGACAACTT TGCTTATCTT 
CAATTCATGT GGAAGGCCGA TGTGGTTGAA AGTTGGATTG CCGATAAAGA AAATCACGTC 
AAATCGGAAG AGTTTGGACG TGATTTGTCT ACTGTTCAAA CATTGCTAAC CAAACAGGAG 
ACATTCGACG CTGGTCTCTC TGCATTCGAA CATGAAGGAA TCCATAATAT CACTGCGTTG 
AAAGATCAAC TAATTAACGC CAATCACGCC CAATCGGCCG CCATCCTCAA GCGCCACGAA 
GATGTGTTGA CCCGCTGGCA GAAATTGCGC GCTGATTCGG AGGCTCGCAA GTATCGTCTA 
TTGGCCATGC AGGAGCAGTT CCGTCAGATT GAAGATCTCT ATCTGACGTT TGCCAAGAAG 
GCTTCCGCTT TCAACTCGTG GTTCGAAAAC GCTGAAGAAG ATCTCACCGA TCCGGTGCGG 
TGCAATTCGA TTGAGGAAAT CAAGGCGCTG CGCGAAGCTC ACGCCCAGTT CCAGGCATCG 
TTGTCATCAG CCCAGTATGA CTTCCAAGCA TTGGCCGATC TAGACCGTAA AATCAAGAGC 
TTCAACGTTG GTCCCAATCC GTACACGTGG TTCACAATGG AAGCTCTAGA AGACACCTGG 
CGTAATCTGC AAAAGATCAT CGAAGAACGT GATGCTGAAT TGGCAAAGGA AGTTCACCGT 
CAAGAAGAGA ACGACAAGCT TCGCAAGGAA TTCGCCAAAC ATGCCAACTT GTTCCACCAG 
TGGCTTACCG AAACAAGAAC ATCTCTTATG GACGGATCCG GTTCGTTGGA GGAACAATTT 
GAAGCGCTTT GTCACAAAGC AAATGAAATT CGCGCTCGTC GTGGAGATCT GAAGAAGATT 
GAAGAGTTGG GTGCCACCTT GGAAGAGCAC CTTATTTTGG ATAACCGCTA TACTGAACAC 
TCAACTGTAG GCCTTGCACA ACAGTGGGAT CAGCTTGACC AACTTGCCAT GCGCATGCAG 
CACAACCTGA GACAACAAAT CCAAGCCCGC AATCAATCTG GCGTCTCGGA GGATTCACTC 
AAAGAATTCT CAATGATGTT CAAGCACTTC GACAAGGACA AGAGCGGCAA GCTCAACCAC 
CAAGAATTCA AATCCTGTTT ACGTGCCCTG GGTTACGATT TACCAATGGT GGAGGAAGGC 
CAGCCCGACC CGGAGTTCGA GGAAATTTTG AATGTGGTGG ACCCGAACCG CGATGGACAT 
GTTTCACTCC AGGAATACAT AGCCTTTATG ATCTCCAAGG AAACGGAGAA CGTACAAAGC 
TACGAGGAAA TCGAAAATGC TTTCCGCGCA ATCACCGCCT CCGATCGTCC TTACGTCACC 
AAGGATGAAC TTTATTCCAA CCTCACCAAG GACATGGCAG ATTACTGCGT ACAGCGGATG 
AAACCGTTCA ACGACCCGAA AACGGGCCAT CCCATCACCG GTGCCCTCGA TTATGTGGAG 
TTCACGCGAA CTCTATTCCA AAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021699210.1
CDS	273..7559
Translation	MEQFTPKEVKILESAEDIQERREQVLNRYNEFKVETRHKREKLEDSRRFQYFKRDSDELESWIHEKLQAASEESYRDPTNLQAKIQKHQAFEAEVSAHSNAIVVLDNTGQEMINQHHFASDTIQRRLDELHRLWELLLSRLAEKGMKLQQALVLVQFLRHCEEVMFWIKDKEAFVTADEFGQDLEHVEVLQRKFDEFQKDMASQEYRVTEVNELADKLLSNGHPERETITRKKEELNEAWQRLKQLAILRQEKLFGAHEIQRFNRDADETVAWIAEKDVVLSSDDYGRDLASVQALQRKHEGVERDLAALEDKVATLGAEAGRLCSIHADHSDQIREKQAEIAAYWQSLTAKAKERKQKLDESYFLHRFLADFRDLVSWINGMKAIISADELAKDVAGAEALLERHQEHKGEIDARGDSFKVTTEAGRQLLEREHYAAAEVQEKLAALESDKSSLLALWEERRILYEQCMDLQLFYRDTEQADTWMAKQEAFLANEDLGDSLDSVEALIKKHEDFEKSLAAQEEKIKALDVFATKLIDGQHYAADDVAQRRSMLLARRSALLEKSSVRRQLLEDSSALQQFERDCDETKGWISEKLKFATDDSYLDPTNLNGKVQKHTNFEHELTANKSRIEDITTNGQNLVEKGHYGADQINSRMQEIVTLWESLVQSSDKKGCKLQEASQQQQFNRTVEDIELWLSEVEGQLMSEDYGKDLTSVQNLQKKHGLLEADVMAHQDRIEGIKVAANKFVESGHFDADNIRNKESALSKRYGALATPMAERKQRLLDSLQVQQLFRDLEDEAAWIREKEPVAASTNRGRDLIGVQNLIKKHQAVLAEINNHENRVAGVIANGEQMLTEQPFATEDIKLRLDAVKDQWNSLKEKANQRKQDLEDSLQAHQYFADANEAESWMREKEPIVSNQDYGKDEDSSEALLKKHEALVSDLEAFGNTIQALQEQAKNCRQQETPVVDITGKECVMALYDYTEKSPREVSMKKGDVLTLLNSNNKDWWKVEVNDRQGFVPAAYIKKIDPGLSASQQNLVDGHSIAKRQAQINSQYDNLLALARERQNKLNETVKAYVLVREAADLSAWIRDKESHAQIKDVGEDLEEVEVMQKKFDDFNDDLKANEVRLAKLNEIAVQLTSLGQTEAALKIKTQIQTLNEEWTVLQNITQERASQLGSAHEVQRFHRDVDETKDWIGEKDTALTNDDLGKDLRGVQTLQRKHEGLERDLAALRDKIRQLDETANRLMQSHPETAEQTYAKQKEINEEWQQVVAKTQQRKEKLLDSYDLQRFLSDYRDLMAWISSMMGLVTSEELANDVTGAEALIERHQNHRAEIDFRYGIPQEHRTEVDARAGTFAAFDQFGAELLQANHYASPEIQEKIENLAKAREDLERAWTARRLQLDQNLDLQLYLRDCEQAENWMSAREAFLNAEEVDSKGDNVEALIKKHEDFDKAINGHEEKIAALQVLADQLIAQEHYAGKLIDDKRADVLDRWRHLKEDLIEKRSRLGDEQTLQQFSRDADEIENWIAEKLQLATEESYKDPANIQSKHQKHQAFEAELAANADRIQSVLAMGSNLIDKNQCSGSEDAVQKRLTQIADQWEYLTQKTTEKSLKLKEANKQRTYIAAVKDLDFWLGEVESLLTSEDAGKDLASVQNLMKKHQLVEADIHAHEDRIKDMNAQADSLVESGQFDSAGIQEKRQSINERYERICNLAAHRQARLNEANTLHQFFRDIADEESWIKEKKLLVGSDDYGRDLTGVQNLKKKHKRLEAELASHEPAIQAVQEAGEKLMDVSNLGVPEIEQRLKALNQAWAELKSLAATRGQKLDESLIYQQFLAKVEEEEAWITEKQQLLSVEDYGDSMAAVQGLLKKHDAFETDFAAHRDRCADIHDNGQTLVTNNNHHAESISQRCTQLDKKLENLQALATRRKSALLDNFAYLQFMWKADVVESWIADKENHVKSEEFGRDLSTVQTLLTKQETFDAGLSAFEHEGIHNITALKDQLINANHAQSAAILKRHEDVLTRWQKLRADSEARKYRLLAMQEQFRQIEDLYLTFAKKASAFNSWFENAEEDLTDPVRCNSIEEIKALREAHAQFQASLSSAQYDFQALADLDRKIKSFNVGPNPYTWFTMEALEDTWRNLQKIIEERDAELAKEVHRQEENDKLRKEFAKHANLFHQWLTETRTSLMDGSGSLEEQFEALCHKANEIRARRGDLKKIEELGATLEEHLILDNRYTEHSTVGLAQQWDQLDQLAMRMQHNLRQQIQARNQSGVSEDSLKEFSMMFKHFDKDKSGKLNHQEFKSCLRALGYDLPMVEEGQPDPEFEEILNVVDPNRDGHVSLQEYIAFMISKETENVQSYEEIENAFRAITASDRPYVTKDELYSNLTKDMADYCVQRMKPFNDPKTGHPITGALDYVEFTRTLFQN

Target ORF information:

RefSeq Version	XM_021843518.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti spectrin alpha chain (LOC5566099), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021843518.1

ORF Insert Sequence:

ATGGAACAGT TTACCCCCAA GGAGGTAAAA ATCCTCGAAA GCGCTGAGGA TATTCAAGAG 
CGCCGTGAAC AGGTGCTCAA CCGATACAAT GAGTTCAAGG TCGAAACGCG CCACAAGCGC 
GAGAAGCTGG AAGATTCGCG TCGTTTCCAG TACTTTAAGC GTGACTCGGA CGAGTTGGAA 
AGCTGGATCC ACGAGAAATT GCAGGCCGCT TCGGAGGAGA GCTATCGCGA TCCTACCAAT 
TTGCAGGCCA AGATTCAGAA GCATCAGGCG TTCGAGGCTG AAGTTTCGGC CCATAGCAAT 
GCGATCGTAG TATTGGACAA CACCGGTCAG GAGATGATCA ATCAGCACCA CTTTGCCTCG 
GACACTATCC AACGTCGCTT GGATGAGTTG CACCGTTTGT GGGAATTGTT GCTGTCCCGT 
TTGGCCGAAA AGGGAATGAA ATTGCAACAG GCTCTGGTCT TGGTGCAATT CTTGCGCCAC 
TGCGAGGAGG TCATGTTCTG GATCAAGGAC AAGGAAGCTT TTGTGACGGC CGATGAGTTC 
GGTCAAGATT TGGAACATGT CGAAGTCTTG CAACGTAAGT TTGACGAGTT CCAGAAGGAC 
ATGGCTTCGC AAGAGTATCG AGTTACTGAA GTCAACGAGT TGGCTGACAA GTTGCTCTCA 
AACGGACACC CTGAGCGTGA AACTATCACT CGCAAGAAGG AAGAATTGAA CGAGGCTTGG 
CAACGCCTGA AGCAGCTGGC TATTCTGCGT CAGGAAAAGC TTTTCGGTGC CCATGAAATT 
CAACGTTTCA ATCGCGATGC TGATGAAACC GTCGCTTGGA TTGCTGAAAA AGACGTTGTT 
CTGTCGTCGG ATGATTATGG ACGTGACTTG GCTAGTGTTC AAGCTTTGCA ACGCAAGCAC 
GAAGGGGTAG AGCGCGACTT GGCTGCTCTG GAAGATAAGG TGGCCACTTT GGGAGCTGAA 
GCCGGTCGCT TATGTAGCAT CCACGCCGAT CATAGTGATC AAATTCGTGA GAAGCAAGCT 
GAAATTGCTG CCTACTGGCA ATCATTGACT GCCAAGGCCA AGGAACGTAA GCAAAAACTG 
GATGAATCAT ATTTCTTGCA CCGTTTCTTG GCTGATTTCC GCGATTTGGT TTCGTGGATC 
AACGGAATGA AGGCCATTAT CTCGGCTGAT GAACTTGCAA AGGATGTAGC TGGAGCTGAG 
GCTCTGCTTG AGCGTCACCA GGAGCACAAA GGAGAAATCG ATGCCCGTGG AGATAGCTTC 
AAGGTTACAA CCGAGGCTGG TCGTCAGCTT TTGGAACGGG AGCATTATGC CGCTGCTGAA 
GTGCAGGAAA AATTGGCTGC TCTAGAGAGC GATAAGAGTT CGCTTCTCGC GCTTTGGGAA 
GAACGTCGCA TTTTGTATGA ACAGTGCATG GATCTACAAC TGTTCTATCG TGACACTGAG 
CAGGCAGATA CTTGGATGGC CAAACAGGAA GCTTTCCTAG CCAATGAAGA TCTAGGAGAT 
TCGCTTGATT CCGTCGAAGC GCTGATTAAG AAGCACGAAG ATTTCGAGAA AAGTTTGGCG 
GCCCAAGAGG AGAAGATCAA GGCTTTGGAT GTTTTCGCTA CAAAGCTCAT CGATGGTCAG 
CATTACGCTG CTGACGATGT TGCCCAACGT CGTTCAATGT TGTTAGCTCG TCGATCAGCC 
TTGCTCGAGA AGTCTTCTGT ACGCCGTCAA CTGCTGGAGG ATTCCAGTGC TTTGCAGCAA 
TTTGAACGTG ACTGCGATGA GACTAAAGGA TGGATCAGCG AAAAGCTTAA ATTTGCCACA 
GATGACAGCT ATTTGGACCC AACCAATCTG AATGGAAAAG TTCAGAAACA TACCAACTTT 
GAGCATGAAT TAACGGCCAA CAAGAGCCGC ATTGAAGACA TCACCACCAA TGGTCAGAAT 
TTAGTCGAGA AGGGACACTA CGGTGCCGAT CAAATCAACT CGCGCATGCA GGAGATTGTC 
ACTTTGTGGG AATCTTTGGT TCAATCATCG GACAAAAAGG GCTGCAAATT GCAGGAAGCA 
TCCCAACAGC AACAATTCAA TCGCACCGTT GAAGATATTG AACTGTGGTT GAGCGAAGTG 
GAAGGCCAAC TAATGTCCGA GGATTATGGC AAAGACCTGA CCAGTGTCCA GAATTTGCAG 
AAGAAACATG GTTTGTTGGA GGCTGATGTC ATGGCCCATC AAGATCGCAT TGAAGGAATT 
AAAGTTGCAG CGAACAAGTT CGTCGAGAGT GGTCATTTCG ATGCCGACAA CATCCGTAAT 
AAGGAATCTG CGCTGTCCAA GCGTTATGGA GCTCTGGCTA CTCCGATGGC TGAACGAAAG 
CAGCGTTTGT TGGATTCCCT ACAGGTGCAG CAACTGTTCC GTGATCTGGA AGACGAAGCT 
GCATGGATCC GTGAGAAGGA ACCTGTCGCT GCATCCACCA ACCGTGGCCG CGATTTAATC 
GGTGTTCAGA ATTTGATTAA GAAGCATCAG GCCGTGTTGG CAGAAATCAA CAATCATGAA 
AATCGCGTCG CCGGAGTCAT CGCGAATGGC GAGCAAATGT TGACTGAGCA GCCTTTTGCC 
ACCGAAGATA TCAAATTGCG TTTGGATGCT GTTAAAGATC AATGGAATTC ATTGAAGGAG 
AAGGCTAATC AACGGAAGCA AGATCTGGAA GATTCGTTGC AGGCCCACCA GTACTTTGCT 
GATGCTAACG AGGCGGAATC TTGGATGCGT GAGAAGGAAC CTATCGTATC GAATCAAGAT 
TACGGCAAGG ACGAAGATTC GTCTGAAGCT TTGTTGAAGA AGCATGAAGC ATTGGTGTCC 
GATTTGGAAG CTTTTGGTAA CACCATTCAA GCTTTGCAAG AACAGGCCAA GAATTGTCGT 
CAGCAGGAAA CACCAGTGGT GGACATTACC GGAAAGGAAT GCGTCATGGC TCTGTATGAT 
TATACCGAGA AATCTCCTCG TGAAGTTTCG ATGAAGAAGG GGGACGTATT AACTCTTTTG 
AACTCGAACA ACAAGGATTG GTGGAAGGTA GAGGTCAACG ATCGTCAGGG ATTCGTTCCG 
GCTGCATACA TTAAGAAGAT CGATCCTGGC CTAAGCGCCA GTCAACAGAA TTTGGTTGAT 
GGCCATTCAA TTGCTAAGCG CCAGGCTCAA ATCAACAGCC AGTACGATAA CTTGCTTGCT 
TTGGCACGTG AACGACAGAA CAAATTGAAT GAAACCGTAA AGGCTTATGT TCTGGTACGT 
GAAGCTGCTG ATTTGTCCGC TTGGATTCGC GACAAGGAAA GCCACGCTCA GATTAAGGAT 
GTTGGCGAAG ATCTGGAAGA AGTTGAAGTT ATGCAGAAGA AGTTTGATGA CTTCAATGAT 
GATCTGAAGG CTAACGAAGT TCGTCTAGCT AAGTTGAACG AGATTGCCGT ACAGCTGACT 
TCGCTAGGTC AAACCGAAGC AGCATTGAAG ATCAAAACTC AAATTCAGAC CTTGAACGAA 
GAGTGGACAG TTTTGCAGAA TATTACCCAG GAAAGGGCTA GTCAACTTGG ATCGGCTCAT 
GAGGTTCAAC GCTTCCATCG TGATGTAGAT GAAACCAAGG ATTGGATTGG CGAGAAGGAC 
ACAGCTTTGA CGAATGACGA TCTTGGTAAG GATCTGCGTG GCGTTCAGAC CCTGCAACGC 
AAGCATGAAG GTTTGGAGCG TGATTTGGCA GCTCTACGAG ACAAAATTCG TCAATTGGAC 
GAAACTGCCA ATCGCCTCAT GCAATCCCAC CCGGAAACCG CCGAGCAGAC TTATGCCAAG 
CAGAAGGAAA TCAACGAGGA ATGGCAGCAG GTTGTTGCCA AAACTCAGCA ACGCAAGGAG 
AAGCTGTTAG ATTCTTATGA TCTACAGCGG TTCCTGAGCG ATTATCGCGA TCTGATGGCT 
TGGATCAGCT CAATGATGGG CTTGGTCACG TCCGAAGAGT TGGCCAACGA TGTCACCGGG 
GCTGAAGCTT TAATCGAAAG ACATCAGAAT CATCGTGCTG AAATTGATTT TCGTTACGGT 
ATCCCGCAGG AACACCGTAC CGAGGTAGAT GCCCGCGCTG GAACCTTCGC TGCGTTCGAT 
CAGTTTGGAG CTGAGCTGTT GCAGGCAAAT CATTATGCGT CACCCGAAAT TCAAGAGAAA 
ATTGAAAACT TGGCCAAGGC ACGTGAGGAT TTGGAGCGTG CCTGGACTGC TCGTCGTCTG 
CAGTTGGACC AGAATCTGGA TCTTCAGCTG TATCTGCGTG ATTGCGAACA GGCTGAGAAC 
TGGATGAGCG CCCGTGAAGC ATTCCTGAAT GCCGAAGAAG TCGACTCCAA GGGCGACAAC 
GTGGAAGCAT TGATTAAGAA GCACGAAGAC TTTGACAAAG CTATCAACGG TCATGAGGAG 
AAAATTGCCG CCCTGCAGGT GCTGGCTGAT CAATTGATTG CCCAGGAGCA CTACGCAGGA 
AAATTAATCG ACGACAAGCG TGCAGATGTG TTGGATCGTT GGCGTCACTT GAAGGAAGAT 
TTGATTGAAA AACGATCTCG CTTGGGAGAT GAGCAAACTC TACAGCAATT CTCACGTGAT 
GCTGATGAAA TCGAAAACTG GATTGCCGAG AAGCTGCAAC TAGCCACGGA GGAAAGTTAC 
AAGGACCCGG CCAATATTCA ATCCAAGCAT CAGAAGCACC AGGCTTTCGA AGCCGAATTG 
GCTGCTAATG CCGATCGTAT TCAGAGCGTG TTGGCGATGG GAAGCAATTT GATCGATAAG 
AATCAATGCA GTGGGTCCGA AGATGCAGTT CAGAAGCGTT TGACACAGAT CGCTGATCAG 
TGGGAATATC TGACCCAGAA AACAACGGAG AAATCCCTGA AGCTGAAGGA AGCTAACAAA 
CAGCGCACTT ATATTGCTGC TGTCAAGGAT TTGGACTTCT GGTTGGGTGA AGTTGAGAGT 
CTTTTGACCT CAGAAGATGC TGGAAAAGAT TTGGCTTCCG TCCAAAATCT TATGAAGAAG 
CACCAGCTGG TAGAGGCTGA CATCCATGCC CATGAAGATC GCATCAAGGA TATGAACGCC 
CAAGCCGACT CATTGGTCGA AAGTGGCCAA TTTGACAGTG CAGGAATTCA AGAAAAGCGT 
CAGTCCATCA ACGAGCGTTA CGAACGTATT TGTAATTTGG CAGCACATCG CCAAGCTCGC 
TTGAACGAAG CCAACACTTT GCATCAATTC TTCCGTGACA TTGCCGACGA GGAATCCTGG 
ATTAAGGAAA AGAAATTGTT GGTTGGTTCG GATGATTATG GTCGCGATTT AACCGGTGTC 
CAAAATCTGA AAAAGAAGCA CAAGCGTCTT GAGGCTGAGT TGGCTTCTCA CGAGCCAGCA 
ATTCAGGCTG TTCAGGAAGC TGGTGAAAAG TTGATGGATG TTTCGAATCT TGGTGTTCCA 
GAAATTGAGC AACGTCTGAA GGCTCTGAAC CAAGCCTGGG CTGAATTGAA GAGCTTGGCT 
GCTACTCGTG GTCAGAAACT GGATGAATCT CTCATTTATC AACAATTCTT AGCTAAGGTT 
GAAGAAGAGG AAGCATGGAT TACCGAGAAG CAACAACTGT TGTCGGTTGA AGATTATGGA 
GATTCGATGG CTGCTGTCCA GGGTCTTTTG AAGAAACATG ACGCCTTCGA GACCGATTTC 
GCCGCTCATC GTGATCGTTG CGCTGACATT CATGACAATG GTCAAACTTT GGTGACCAAT 
AATAATCATC ACGCCGAAAG CATTTCGCAG CGCTGCACAC AACTGGATAA GAAGTTGGAG 
AATCTGCAGG CGTTGGCTAC TCGTCGCAAG AGCGCCCTTC TGGACAACTT TGCTTATCTT 
CAATTCATGT GGAAGGCCGA TGTGGTTGAA AGTTGGATTG CCGATAAAGA AAATCACGTC 
AAATCGGAAG AGTTTGGACG TGATTTGTCT ACTGTTCAAA CATTGCTAAC CAAACAGGAG 
ACATTCGACG CTGGTCTCTC TGCATTCGAA CATGAAGGAA TCCATAATAT CACTGCGTTG 
AAAGATCAAC TAATTAACGC CAATCACGCC CAATCGGCCG CCATCCTCAA GCGCCACGAA 
GATGTGTTGA CCCGCTGGCA GAAATTGCGC GCTGATTCGG AGGCTCGCAA GTATCGTCTA 
TTGGCCATGC AGGAGCAGTT CCGTCAGATT GAAGATCTCT ATCTGACGTT TGCCAAGAAG 
GCTTCCGCTT TCAACTCGTG GTTCGAAAAC GCTGAAGAAG ATCTCACCGA TCCGGTGCGG 
TGCAATTCGA TTGAGGAAAT CAAGGCGCTG CGCGAAGCTC ACGCCCAGTT CCAGGCATCG 
TTGTCATCAG CCCAGTATGA CTTCCAAGCA TTGGCCGATC TAGACCGTAA AATCAAGAGC 
TTCAACGTTG GTCCCAATCC GTACACGTGG TTCACAATGG AAGCTCTAGA AGACACCTGG 
CGTAATCTGC AAAAGATCAT CGAAGAACGT GATGCTGAAT TGGCAAAGGA AGTTCACCGT 
CAAGAAGAGA ACGACAAGCT TCGCAAGGAA TTCGCCAAAC ATGCCAACTT GTTCCACCAG 
TGGCTTACCG AAACAAGAAC ATCTCTTATG GACGGATCCG GTTCGTTGGA GGAACAATTT 
GAAGCGCTTT GTCACAAAGC AAATGAAATT CGCGCTCGTC GTGGAGATCT GAAGAAGATT 
GAAGAGTTGG GTGCCACCTT GGAAGAGCAC CTTATTTTGG ATAACCGCTA TACTGAACAC 
TCAACTGTAG GCCTTGCACA ACAGTGGGAT CAGCTTGACC AACTTGCCAT GCGCATGCAG 
CACAACCTGA GACAACAAAT CCAAGCCCGC AATCAATCTG GCGTCTCGGA GGATTCACTC 
AAAGAATTCT CAATGATGTT CAAGCACTTC GACAAGGACA AGAGCGGCAA GCTCAACCAC 
CAAGAATTCA AATCCTGTTT ACGTGCCCTG GGTTACGATT TACCAATGGT GGAGGAAGGC 
CAGCCCGACC CGGAGTTCGA GGAAATTTTG AATGTGGTGG ACCCGAACCG CGATGGACAT 
GTTTCACTCC AGGAATACAT AGCCTTTATG ATCTCCAAGG AAACGGAGAA CGTACAAAGC 
TACGAGGAAA TCGAAAATGC TTTCCGCGCA ATCACCGCCT CCGATCGTCC TTACGTCACC 
AAGGATGAAC TTTATTCCAA CCTCACCAAG GACATGGCAG ATTACTGCGT ACAGCGGATG 
AAACCGTTCA ACGACCCGAA AACGGGCCAT CCCATCACCG GTGCCCTCGA TTATGTGGAG 
TTCACGCGAA CTCTATTCCA AAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5566099 ( XM_021843519.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021699211.1 Aedes aegypti spectrin alpha chain (LOC5566099), transcript variant X3, mRNA.

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CloneID	OAe121123
Clone ID Related Accession (Same CDS sequence)	XM_021843519.1
Accession Version	XM_021843519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	spectrin alpha chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAGT TTACCCCCAA GGAGGTAAAA ATCCTCGAAA GCGCTGAGGA TATTCAAGAG 
CGCCGTGAAC AGGTGCTCAA CCGATACAAT GAGTTCAAGG TCGAAACGCG CCACAAGCGC 
GAGAAGCTGG AAGATTCGCG TCGTTTCCAG TACTTTAAGC GTGACTCGGA CGAGTTGGAA 
AGCTGGATCC ACGAGAAATT GCAGGCCGCT TCGGAGGAGA GCTATCGCGA TCCTACCAAT 
TTGCAGGCCA AGATTCAGAA GCATCAGGCG TTCGAGGCTG AAGTTTCGGC CCATAGCAAT 
GCGATCGTAG TATTGGACAA CACCGGTCAG GAGATGATCA ATCAGCACCA CTTTGCCTCG 
GACACTATCC AACGTCGCTT GGATGAGTTG CACCGTTTGT GGGAATTGTT GCTGTCCCGT 
TTGGCCGAAA AGGGAATGAA ATTGCAACAG GCTCTGGTCT TGGTGCAATT CTTGCGCCAC 
TGCGAGGAGG TCATGTTCTG GATCAAGGAC AAGGAAGCTT TTGTGACGGC CGATGAGTTC 
GGTCAAGATT TGGAACATGT CGAAGTCTTG CAACGTAAGT TTGACGAGTT CCAGAAGGAC 
ATGGCTTCGC AAGAGTATCG AGTTACTGAA GTCAACGAGT TGGCTGACAA GTTGCTCTCA 
AACGGACACC CTGAGCGTGA AACTATCACT CGCAAGAAGG AAGAATTGAA CGAGGCTTGG 
CAACGCCTGA AGCAGCTGGC TATTCTGCGT CAGGAAAAGC TTTTCGGTGC CCATGAAATT 
CAACGTTTCA ATCGCGATGC TGATGAAACC GTCGCTTGGA TTGCTGAAAA AGACGTTGTT 
CTGTCGTCGG ATGATTATGG ACGTGACTTG GCTAGTGTTC AAGCTTTGCA ACGCAAGCAC 
GAAGGGGTAG AGCGCGACTT GGCTGCTCTG GAAGATAAGG TGGCCACTTT GGGAGCTGAA 
GCCGGTCGCT TATGTAGCAT CCACGCCGAT CATAGTGATC AAATTCGTGA GAAGCAAGCT 
GAAATTGCTG CCTACTGGCA ATCATTGACT GCCAAGGCCA AGGAACGTAA GCAAAAACTG 
GATGAATCAT ATTTCTTGCA CCGTTTCTTG GCTGATTTCC GCGATTTGGT TTCGTGGATC 
AACGGAATGA AGGCCATTAT CTCGGCTGAT GAACTTGCAA AGGATGTAGC TGGAGCTGAG 
GCTCTGCTTG AGCGTCACCA GGAGCACAAA GGAGAAATCG ATGCCCGTGG AGATAGCTTC 
AAGGTTACAA CCGAGGCTGG TCGTCAGCTT TTGGAACGGG AGCATTATGC CGCTGCTGAA 
GTGCAGGAAA AATTGGCTGC TCTAGAGAGC GATAAGAGTT CGCTTCTCGC GCTTTGGGAA 
GAACGTCGCA TTTTGTATGA ACAGTGCATG GATCTACAAC TGTTCTATCG TGACACTGAG 
CAGGCAGATA CTTGGATGGC CAAACAGGAA GCTTTCCTAG CCAATGAAGA TCTAGGAGAT 
TCGCTTGATT CCGTCGAAGC GCTGATTAAG AAGCACGAAG ATTTCGAGAA AAGTTTGGCG 
GCCCAAGAGG AGAAGATCAA GGCTTTGGAT GTTTTCGCTA CAAAGCTCAT CGATGGTCAG 
CATTACGCTG CTGACGATGT TGCCCAACGT CGTTCAATGT TGTTAGCTCG TCGATCAGCC 
TTGCTCGAGA AGTCTTCTGT ACGCCGTCAA CTGCTGGAGG ATTCCAGTGC TTTGCAGCAA 
TTTGAACGTG ACTGCGATGA GACTAAAGGA TGGATCAGCG AAAAGCTTAA ATTTGCCACA 
GATGACAGCT ATTTGGACCC AACCAATCTG AATGGAAAAG TTCAGAAACA TACCAACTTT 
GAGCATGAAT TAACGGCCAA CAAGAGCCGC ATTGAAGACA TCACCACCAA TGGTCAGAAT 
TTAGTCGAGA AGGGACACTA CGGTGCCGAT CAAATCAACT CGCGCATGCA GGAGATTGTC 
ACTTTGTGGG AATCTTTGGT TCAATCATCG GACAAAAAGG GCTGCAAATT GCAGGAAGCA 
TCCCAACAGC AACAATTCAA TCGCACCGTT GAAGATATTG AACTGTGGTT GAGCGAAGTG 
GAAGGCCAAC TAATGTCCGA GGATTATGGC AAAGACCTGA CCAGTGTCCA GAATTTGCAG 
AAGAAACATG GTTTGTTGGA GGCTGATGTC ATGGCCCATC AAGATCGCAT TGAAGGAATT 
AAAGTTGCAG CGAACAAGTT CGTCGAGAGT GGTCATTTCG ATGCCGACAA CATCCGTAAT 
AAGGAATCTG CGCTGTCCAA GCGTTATGGA GCTCTGGCTA CTCCGATGGC TGAACGAAAG 
CAGCGTTTGT TGGATTCCCT ACAGGTGCAG CAACTGTTCC GTGATCTGGA AGACGAAGCT 
GCATGGATCC GTGAGAAGGA ACCTGTCGCT GCATCCACCA ACCGTGGCCG CGATTTAATC 
GGTGTTCAGA ATTTGATTAA GAAGCATCAG GCCGTGTTGG CAGAAATCAA CAATCATGAA 
AATCGCGTCG CCGGAGTCAT CGCGAATGGC GAGCAAATGT TGACTGAGCA GCCTTTTGCC 
ACCGAAGATA TCAAATTGCG TTTGGATGCT GTTAAAGATC AATGGAATTC ATTGAAGGAG 
AAGGCTAATC AACGGAAGCA AGATCTGGAA GATTCGTTGC AGGCCCACCA GTACTTTGCT 
GATGCTAACG AGGCGGAATC TTGGATGCGT GAGAAGGAAC CTATCGTATC GAATCAAGAT 
TACGGCAAGG ACGAAGATTC GTCTGAAGCT TTGTTGAAGA AGCATGAAGC ATTGGTGTCC 
GATTTGGAAG CTTTTGGTAA CACCATTCAA GCTTTGCAAG AACAGGCCAA GAATTGTCGT 
CAGCAGGAAA CACCAGTGGT GGACATTACC GGAAAGGAAT GCGTCATGGC TCTGTATGAT 
TATACCGAGA AATCTCCTCG TGAAGTTTCG ATGAAGAAGG GGGACGTATT AACTCTTTTG 
AACTCGAACA ACAAGGATTG GTGGAAGGTA GAGGTCAACG ATCGTCAGGG ATTCGTTCCG 
GCTGCATACA TTAAGAAGAT CGATCCTGGC CTAAGCGCCA GTCAACAGAA TTTGGTTGAT 
GGCCATTCAA TTGCTAAGCG CCAGGCTCAA ATCAACAGCC AGTACGATAA CTTGCTTGCT 
TTGGCACGTG AACGACAGAA CAAATTGAAT GAAACCGTAA AGGCTTATGT TCTGGTACGT 
GAAGCTGCTG ATTTGTCCGC TTGGATTCGC GACAAGGAAA GCCACGCTCA GATTAAGGAT 
GTTGGCGAAG ATCTGGAAGA AGTTGAAGTT ATGCAGAAGA AGTTTGATGA CTTCAATGAT 
GATCTGAAGG CTAACGAAGT TCGTCTAGCT AAGTTGAACG AGATTGCCGT ACAGCTGACT 
TCGCTAGGTC AAACCGAAGC AGCATTGAAG ATCAAAACTC AAATTCAGAC CTTGAACGAA 
GAGTGGACAG TTTTGCAGAA TATTACCCAG GAAAGGGCTA GTCAACTTGG ATCGGCTCAT 
GAGGTTCAAC GCTTCCATCG TGATGTAGAT GAAACCAAGG ATTGGATTGG CGAGAAGGAC 
ACAGCTTTGA CGAATGACGA TCTTGGTAAG GATCTGCGTG GCGTTCAGAC CCTGCAACGC 
AAGCATGAAG GTTTGGAGCG TGATTTGGCA GCTCTACGAG ACAAAATTCG TCAATTGGAC 
GAAACTGCCA ATCGCCTCAT GCAATCCCAC CCGGAAACCG CCGAGCAGAC TTATGCCAAG 
CAGAAGGAAA TCAACGAGGA ATGGCAGCAG GTTGTTGCCA AAACTCAGCA ACGCAAGGAG 
AAGCTGTTAG ATTCTTATGA TCTACAGCGG TTCCTGAGCG ATTATCGCGA TCTGATGGCT 
TGGATCAGCT CAATGATGGG CTTGGTCACG TCCGAAGAGT TGGCCAACGA TGTCACCGGG 
GCTGAAGCTT TAATCGAAAG ACATCAGGAA CACCGTACCG AGGTAGATGC CCGCGCTGGA 
ACCTTCGCTG CGTTCGATCA GTTTGGAGCT GAGCTGTTGC AGGCAAATCA TTATGCGTCA 
CCCGAAATTC AAGAGAAAAT TGAAAACTTG GCCAAGGCAC GTGAGGATTT GGAGCGTGCC 
TGGACTGCTC GTCGTCTGCA GTTGGACCAG AATCTGGATC TTCAGCTGTA TCTGCGTGAT 
TGCGAACAGG CTGAGAACTG GATGAGCGCC CGTGAAGCAT TCCTGAATGC CGAAGAAGTC 
GACTCCAAGG GCGACAACGT GGAAGCATTG ATTAAGAAGC ACGAAGACTT TGACAAAGCT 
ATCAACGGTC ATGAGGAGAA AATTGCCGCC CTGCAGGTGC TGGCTGATCA ATTGATTGCC 
CAGGAGCACT ACGCAGGAAA ATTAATCGAC GACAAGCGTG CAGATGTGTT GGATCGTTGG 
CGTCACTTGA AGGAAGATTT GATTGAAAAA CGATCTCGCT TGGGAGATGA GCAAACTCTA 
CAGCAATTCT CACGTGATGC TGATGAAATC GAAAACTGGA TTGCCGAGAA GCTGCAACTA 
GCCACGGAGG AAAGTTACAA GGACCCGGCC AATATTCAAT CCAAGCATCA GAAGCACCAG 
GCTTTCGAAG CCGAATTGGC TGCTAATGCC GATCGTATTC AGAGCGTGTT GGCGATGGGA 
AGCAATTTGA TCGATAAGAA TCAATGCAGT GGGTCCGAAG ATGCAGTTCA GAAGCGTTTG 
ACACAGATCG CTGATCAGTG GGAATATCTG ACCCAGAAAA CAACGGAGAA ATCCCTGAAG 
CTGAAGGAAG CTAACAAACA GCGCACTTAT ATTGCTGCTG TCAAGGATTT GGACTTCTGG 
TTGGGTGAAG TTGAGAGTCT TTTGACCTCA GAAGATGCTG GAAAAGATTT GGCTTCCGTC 
CAAAATCTTA TGAAGAAGCA CCAGCTGGTA GAGGCTGACA TCCATGCCCA TGAAGATCGC 
ATCAAGGATA TGAACGCCCA AGCCGACTCA TTGGTCGAAA GTGGCCAATT TGACAGTGCA 
GGAATTCAAG AAAAGCGTCA GTCCATCAAC GAGCGTTACG AACGTATTTG TAATTTGGCA 
GCACATCGCC AAGCTCGCTT GAACGAAGCC AACACTTTGC ATCAATTCTT CCGTGACATT 
GCCGACGAGG AATCCTGGAT TAAGGAAAAG AAATTGTTGG TTGGTTCGGA TGATTATGGT 
CGCGATTTAA CCGGTGTCCA AAATCTGAAA AAGAAGCACA AGCGTCTTGA GGCTGAGTTG 
GCTTCTCACG AGCCAGCAAT TCAGGCTGTT CAGGAAGCTG GTGAAAAGTT GATGGATGTT 
TCGAATCTTG GTGTTCCAGA AATTGAGCAA CGTCTGAAGG CTCTGAACCA AGCCTGGGCT 
GAATTGAAGA GCTTGGCTGC TACTCGTGGT CAGAAACTGG ATGAATCTCT CATTTATCAA 
CAATTCTTAG CTAAGGTTGA AGAAGAGGAA GCATGGATTA CCGAGAAGCA ACAACTGTTG 
TCGGTTGAAG ATTATGGAGA TTCGATGGCT GCTGTCCAGG GTCTTTTGAA GAAACATGAC 
GCCTTCGAGA CCGATTTCGC CGCTCATCGT GATCGTTGCG CTGACATTCA TGACAATGGT 
CAAACTTTGG TGACCAATAA TAATCATCAC GCCGAAAGCA TTTCGCAGCG CTGCACACAA 
CTGGATAAGA AGTTGGAGAA TCTGCAGGCG TTGGCTACTC GTCGCAAGAG CGCCCTTCTG 
GACAACTTTG CTTATCTTCA ATTCATGTGG AAGGCCGATG TGGTTGAAAG TTGGATTGCC 
GATAAAGAAA ATCACGTCAA ATCGGAAGAG TTTGGACGTG ATTTGTCTAC TGTTCAAACA 
TTGCTAACCA AACAGGAGAC ATTCGACGCT GGTCTCTCTG CATTCGAACA TGAAGGAATC 
CATAATATCA CTGCGTTGAA AGATCAACTA ATTAACGCCA ATCACGCCCA ATCGGCCGCC 
ATCCTCAAGC GCCACGAAGA TGTGTTGACC CGCTGGCAGA AATTGCGCGC TGATTCGGAG 
GCTCGCAAGT ATCGTCTATT GGCCATGCAG GAGCAGTTCC GTCAGATTGA AGATCTCTAT 
CTGACGTTTG CCAAGAAGGC TTCCGCTTTC AACTCGTGGT TCGAAAACGC TGAAGAAGAT 
CTCACCGATC CGGTGCGGTG CAATTCGATT GAGGAAATCA AGGCGCTGCG CGAAGCTCAC 
GCCCAGTTCC AGGCATCGTT GTCATCAGCC CAGTATGACT TCCAAGCATT GGCCGATCTA 
GACCGTAAAA TCAAGAGCTT CAACGTTGGT CCCAATCCGT ACACGTGGTT CACAATGGAA 
GCTCTAGAAG ACACCTGGCG TAATCTGCAA AAGATCATCG AAGAACGTGA TGCTGAATTG 
GCAAAGGAAG TTCACCGTCA AGAAGAGAAC GACAAGCTTC GCAAGGAATT CGCCAAACAT 
GCCAACTTGT TCCACCAGTG GCTTACCGAA ACAAGATACA GCATTCTGGG ATGGGACAAA 
AATGGAACAT CTCTTATGGA CGGATCCGGT TCGTTGGAGG AACAATTTGA AGCGCTTTGT 
CACAAAGCAA ATGAAATTCG CGCTCGTCGT GGAGATCTGA AGAAGATTGA AGAGTTGGGT 
GCCACCTTGG AAGAGCACCT TATTTTGGAT AACCGCTATA CTGAACACTC AACTGTAGGC 
CTTGCACAAC AGTGGGATCA GCTTGACCAA CTTGCCATGC GCATGCAGCA CAACCTGAGA 
CAACAAATCC AAGCCCGCAA TCAATCTGGC GTCTCGGAGG ATTCACTCAA AGAATTCTCA 
ATGATGTTCA AGCACTTCGA CAAGGACAAG AGCGGCAAGC TCAACCACCA AGAATTCAAA 
TCCTGTTTAC GTGCCCTGGG TTACGATTTA CCAATGGTGG AGGAAGGCCA GCCCGACCCG 
GAGTTCGAGG AAATTTTGAA TGTGGTGGAC CCGAACCGCG ATGGACATGT TTCACTCCAG 
GAATACATAG CCTTTATGAT CTCCAAGGAA ACGGAGAACG TACAAAGCTA CGAGGAAATC 
GAAAATGCTT TCCGCGCAAT CACCGCCTCC GATCGTCCTT ACGTCACCAA GGATGAACTT 
TATTCCAACC TCACCAAGGA CATGGCAGAT TACTGCGTAC AGCGGATGAA ACCGTTCAAC 
GACCCGAAAA CGGGCCATCC CATCACCGGT GCCCTCGATT ATGTGGAGTT CACGCGAACT 
CTATTCCAAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021699211.1
CDS	273..7547
Translation	MEQFTPKEVKILESAEDIQERREQVLNRYNEFKVETRHKREKLEDSRRFQYFKRDSDELESWIHEKLQAASEESYRDPTNLQAKIQKHQAFEAEVSAHSNAIVVLDNTGQEMINQHHFASDTIQRRLDELHRLWELLLSRLAEKGMKLQQALVLVQFLRHCEEVMFWIKDKEAFVTADEFGQDLEHVEVLQRKFDEFQKDMASQEYRVTEVNELADKLLSNGHPERETITRKKEELNEAWQRLKQLAILRQEKLFGAHEIQRFNRDADETVAWIAEKDVVLSSDDYGRDLASVQALQRKHEGVERDLAALEDKVATLGAEAGRLCSIHADHSDQIREKQAEIAAYWQSLTAKAKERKQKLDESYFLHRFLADFRDLVSWINGMKAIISADELAKDVAGAEALLERHQEHKGEIDARGDSFKVTTEAGRQLLEREHYAAAEVQEKLAALESDKSSLLALWEERRILYEQCMDLQLFYRDTEQADTWMAKQEAFLANEDLGDSLDSVEALIKKHEDFEKSLAAQEEKIKALDVFATKLIDGQHYAADDVAQRRSMLLARRSALLEKSSVRRQLLEDSSALQQFERDCDETKGWISEKLKFATDDSYLDPTNLNGKVQKHTNFEHELTANKSRIEDITTNGQNLVEKGHYGADQINSRMQEIVTLWESLVQSSDKKGCKLQEASQQQQFNRTVEDIELWLSEVEGQLMSEDYGKDLTSVQNLQKKHGLLEADVMAHQDRIEGIKVAANKFVESGHFDADNIRNKESALSKRYGALATPMAERKQRLLDSLQVQQLFRDLEDEAAWIREKEPVAASTNRGRDLIGVQNLIKKHQAVLAEINNHENRVAGVIANGEQMLTEQPFATEDIKLRLDAVKDQWNSLKEKANQRKQDLEDSLQAHQYFADANEAESWMREKEPIVSNQDYGKDEDSSEALLKKHEALVSDLEAFGNTIQALQEQAKNCRQQETPVVDITGKECVMALYDYTEKSPREVSMKKGDVLTLLNSNNKDWWKVEVNDRQGFVPAAYIKKIDPGLSASQQNLVDGHSIAKRQAQINSQYDNLLALARERQNKLNETVKAYVLVREAADLSAWIRDKESHAQIKDVGEDLEEVEVMQKKFDDFNDDLKANEVRLAKLNEIAVQLTSLGQTEAALKIKTQIQTLNEEWTVLQNITQERASQLGSAHEVQRFHRDVDETKDWIGEKDTALTNDDLGKDLRGVQTLQRKHEGLERDLAALRDKIRQLDETANRLMQSHPETAEQTYAKQKEINEEWQQVVAKTQQRKEKLLDSYDLQRFLSDYRDLMAWISSMMGLVTSEELANDVTGAEALIERHQEHRTEVDARAGTFAAFDQFGAELLQANHYASPEIQEKIENLAKAREDLERAWTARRLQLDQNLDLQLYLRDCEQAENWMSAREAFLNAEEVDSKGDNVEALIKKHEDFDKAINGHEEKIAALQVLADQLIAQEHYAGKLIDDKRADVLDRWRHLKEDLIEKRSRLGDEQTLQQFSRDADEIENWIAEKLQLATEESYKDPANIQSKHQKHQAFEAELAANADRIQSVLAMGSNLIDKNQCSGSEDAVQKRLTQIADQWEYLTQKTTEKSLKLKEANKQRTYIAAVKDLDFWLGEVESLLTSEDAGKDLASVQNLMKKHQLVEADIHAHEDRIKDMNAQADSLVESGQFDSAGIQEKRQSINERYERICNLAAHRQARLNEANTLHQFFRDIADEESWIKEKKLLVGSDDYGRDLTGVQNLKKKHKRLEAELASHEPAIQAVQEAGEKLMDVSNLGVPEIEQRLKALNQAWAELKSLAATRGQKLDESLIYQQFLAKVEEEEAWITEKQQLLSVEDYGDSMAAVQGLLKKHDAFETDFAAHRDRCADIHDNGQTLVTNNNHHAESISQRCTQLDKKLENLQALATRRKSALLDNFAYLQFMWKADVVESWIADKENHVKSEEFGRDLSTVQTLLTKQETFDAGLSAFEHEGIHNITALKDQLINANHAQSAAILKRHEDVLTRWQKLRADSEARKYRLLAMQEQFRQIEDLYLTFAKKASAFNSWFENAEEDLTDPVRCNSIEEIKALREAHAQFQASLSSAQYDFQALADLDRKIKSFNVGPNPYTWFTMEALEDTWRNLQKIIEERDAELAKEVHRQEENDKLRKEFAKHANLFHQWLTETRYSILGWDKNGTSLMDGSGSLEEQFEALCHKANEIRARRGDLKKIEELGATLEEHLILDNRYTEHSTVGLAQQWDQLDQLAMRMQHNLRQQIQARNQSGVSEDSLKEFSMMFKHFDKDKSGKLNHQEFKSCLRALGYDLPMVEEGQPDPEFEEILNVVDPNRDGHVSLQEYIAFMISKETENVQSYEEIENAFRAITASDRPYVTKDELYSNLTKDMADYCVQRMKPFNDPKTGHPITGALDYVEFTRTLFQN

Target ORF information:

RefSeq Version	XM_021843519.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti spectrin alpha chain (LOC5566099), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021843519.1

ORF Insert Sequence:

ATGGAACAGT TTACCCCCAA GGAGGTAAAA ATCCTCGAAA GCGCTGAGGA TATTCAAGAG 
CGCCGTGAAC AGGTGCTCAA CCGATACAAT GAGTTCAAGG TCGAAACGCG CCACAAGCGC 
GAGAAGCTGG AAGATTCGCG TCGTTTCCAG TACTTTAAGC GTGACTCGGA CGAGTTGGAA 
AGCTGGATCC ACGAGAAATT GCAGGCCGCT TCGGAGGAGA GCTATCGCGA TCCTACCAAT 
TTGCAGGCCA AGATTCAGAA GCATCAGGCG TTCGAGGCTG AAGTTTCGGC CCATAGCAAT 
GCGATCGTAG TATTGGACAA CACCGGTCAG GAGATGATCA ATCAGCACCA CTTTGCCTCG 
GACACTATCC AACGTCGCTT GGATGAGTTG CACCGTTTGT GGGAATTGTT GCTGTCCCGT 
TTGGCCGAAA AGGGAATGAA ATTGCAACAG GCTCTGGTCT TGGTGCAATT CTTGCGCCAC 
TGCGAGGAGG TCATGTTCTG GATCAAGGAC AAGGAAGCTT TTGTGACGGC CGATGAGTTC 
GGTCAAGATT TGGAACATGT CGAAGTCTTG CAACGTAAGT TTGACGAGTT CCAGAAGGAC 
ATGGCTTCGC AAGAGTATCG AGTTACTGAA GTCAACGAGT TGGCTGACAA GTTGCTCTCA 
AACGGACACC CTGAGCGTGA AACTATCACT CGCAAGAAGG AAGAATTGAA CGAGGCTTGG 
CAACGCCTGA AGCAGCTGGC TATTCTGCGT CAGGAAAAGC TTTTCGGTGC CCATGAAATT 
CAACGTTTCA ATCGCGATGC TGATGAAACC GTCGCTTGGA TTGCTGAAAA AGACGTTGTT 
CTGTCGTCGG ATGATTATGG ACGTGACTTG GCTAGTGTTC AAGCTTTGCA ACGCAAGCAC 
GAAGGGGTAG AGCGCGACTT GGCTGCTCTG GAAGATAAGG TGGCCACTTT GGGAGCTGAA 
GCCGGTCGCT TATGTAGCAT CCACGCCGAT CATAGTGATC AAATTCGTGA GAAGCAAGCT 
GAAATTGCTG CCTACTGGCA ATCATTGACT GCCAAGGCCA AGGAACGTAA GCAAAAACTG 
GATGAATCAT ATTTCTTGCA CCGTTTCTTG GCTGATTTCC GCGATTTGGT TTCGTGGATC 
AACGGAATGA AGGCCATTAT CTCGGCTGAT GAACTTGCAA AGGATGTAGC TGGAGCTGAG 
GCTCTGCTTG AGCGTCACCA GGAGCACAAA GGAGAAATCG ATGCCCGTGG AGATAGCTTC 
AAGGTTACAA CCGAGGCTGG TCGTCAGCTT TTGGAACGGG AGCATTATGC CGCTGCTGAA 
GTGCAGGAAA AATTGGCTGC TCTAGAGAGC GATAAGAGTT CGCTTCTCGC GCTTTGGGAA 
GAACGTCGCA TTTTGTATGA ACAGTGCATG GATCTACAAC TGTTCTATCG TGACACTGAG 
CAGGCAGATA CTTGGATGGC CAAACAGGAA GCTTTCCTAG CCAATGAAGA TCTAGGAGAT 
TCGCTTGATT CCGTCGAAGC GCTGATTAAG AAGCACGAAG ATTTCGAGAA AAGTTTGGCG 
GCCCAAGAGG AGAAGATCAA GGCTTTGGAT GTTTTCGCTA CAAAGCTCAT CGATGGTCAG 
CATTACGCTG CTGACGATGT TGCCCAACGT CGTTCAATGT TGTTAGCTCG TCGATCAGCC 
TTGCTCGAGA AGTCTTCTGT ACGCCGTCAA CTGCTGGAGG ATTCCAGTGC TTTGCAGCAA 
TTTGAACGTG ACTGCGATGA GACTAAAGGA TGGATCAGCG AAAAGCTTAA ATTTGCCACA 
GATGACAGCT ATTTGGACCC AACCAATCTG AATGGAAAAG TTCAGAAACA TACCAACTTT 
GAGCATGAAT TAACGGCCAA CAAGAGCCGC ATTGAAGACA TCACCACCAA TGGTCAGAAT 
TTAGTCGAGA AGGGACACTA CGGTGCCGAT CAAATCAACT CGCGCATGCA GGAGATTGTC 
ACTTTGTGGG AATCTTTGGT TCAATCATCG GACAAAAAGG GCTGCAAATT GCAGGAAGCA 
TCCCAACAGC AACAATTCAA TCGCACCGTT GAAGATATTG AACTGTGGTT GAGCGAAGTG 
GAAGGCCAAC TAATGTCCGA GGATTATGGC AAAGACCTGA CCAGTGTCCA GAATTTGCAG 
AAGAAACATG GTTTGTTGGA GGCTGATGTC ATGGCCCATC AAGATCGCAT TGAAGGAATT 
AAAGTTGCAG CGAACAAGTT CGTCGAGAGT GGTCATTTCG ATGCCGACAA CATCCGTAAT 
AAGGAATCTG CGCTGTCCAA GCGTTATGGA GCTCTGGCTA CTCCGATGGC TGAACGAAAG 
CAGCGTTTGT TGGATTCCCT ACAGGTGCAG CAACTGTTCC GTGATCTGGA AGACGAAGCT 
GCATGGATCC GTGAGAAGGA ACCTGTCGCT GCATCCACCA ACCGTGGCCG CGATTTAATC 
GGTGTTCAGA ATTTGATTAA GAAGCATCAG GCCGTGTTGG CAGAAATCAA CAATCATGAA 
AATCGCGTCG CCGGAGTCAT CGCGAATGGC GAGCAAATGT TGACTGAGCA GCCTTTTGCC 
ACCGAAGATA TCAAATTGCG TTTGGATGCT GTTAAAGATC AATGGAATTC ATTGAAGGAG 
AAGGCTAATC AACGGAAGCA AGATCTGGAA GATTCGTTGC AGGCCCACCA GTACTTTGCT 
GATGCTAACG AGGCGGAATC TTGGATGCGT GAGAAGGAAC CTATCGTATC GAATCAAGAT 
TACGGCAAGG ACGAAGATTC GTCTGAAGCT TTGTTGAAGA AGCATGAAGC ATTGGTGTCC 
GATTTGGAAG CTTTTGGTAA CACCATTCAA GCTTTGCAAG AACAGGCCAA GAATTGTCGT 
CAGCAGGAAA CACCAGTGGT GGACATTACC GGAAAGGAAT GCGTCATGGC TCTGTATGAT 
TATACCGAGA AATCTCCTCG TGAAGTTTCG ATGAAGAAGG GGGACGTATT AACTCTTTTG 
AACTCGAACA ACAAGGATTG GTGGAAGGTA GAGGTCAACG ATCGTCAGGG ATTCGTTCCG 
GCTGCATACA TTAAGAAGAT CGATCCTGGC CTAAGCGCCA GTCAACAGAA TTTGGTTGAT 
GGCCATTCAA TTGCTAAGCG CCAGGCTCAA ATCAACAGCC AGTACGATAA CTTGCTTGCT 
TTGGCACGTG AACGACAGAA CAAATTGAAT GAAACCGTAA AGGCTTATGT TCTGGTACGT 
GAAGCTGCTG ATTTGTCCGC TTGGATTCGC GACAAGGAAA GCCACGCTCA GATTAAGGAT 
GTTGGCGAAG ATCTGGAAGA AGTTGAAGTT ATGCAGAAGA AGTTTGATGA CTTCAATGAT 
GATCTGAAGG CTAACGAAGT TCGTCTAGCT AAGTTGAACG AGATTGCCGT ACAGCTGACT 
TCGCTAGGTC AAACCGAAGC AGCATTGAAG ATCAAAACTC AAATTCAGAC CTTGAACGAA 
GAGTGGACAG TTTTGCAGAA TATTACCCAG GAAAGGGCTA GTCAACTTGG ATCGGCTCAT 
GAGGTTCAAC GCTTCCATCG TGATGTAGAT GAAACCAAGG ATTGGATTGG CGAGAAGGAC 
ACAGCTTTGA CGAATGACGA TCTTGGTAAG GATCTGCGTG GCGTTCAGAC CCTGCAACGC 
AAGCATGAAG GTTTGGAGCG TGATTTGGCA GCTCTACGAG ACAAAATTCG TCAATTGGAC 
GAAACTGCCA ATCGCCTCAT GCAATCCCAC CCGGAAACCG CCGAGCAGAC TTATGCCAAG 
CAGAAGGAAA TCAACGAGGA ATGGCAGCAG GTTGTTGCCA AAACTCAGCA ACGCAAGGAG 
AAGCTGTTAG ATTCTTATGA TCTACAGCGG TTCCTGAGCG ATTATCGCGA TCTGATGGCT 
TGGATCAGCT CAATGATGGG CTTGGTCACG TCCGAAGAGT TGGCCAACGA TGTCACCGGG 
GCTGAAGCTT TAATCGAAAG ACATCAGGAA CACCGTACCG AGGTAGATGC CCGCGCTGGA 
ACCTTCGCTG CGTTCGATCA GTTTGGAGCT GAGCTGTTGC AGGCAAATCA TTATGCGTCA 
CCCGAAATTC AAGAGAAAAT TGAAAACTTG GCCAAGGCAC GTGAGGATTT GGAGCGTGCC 
TGGACTGCTC GTCGTCTGCA GTTGGACCAG AATCTGGATC TTCAGCTGTA TCTGCGTGAT 
TGCGAACAGG CTGAGAACTG GATGAGCGCC CGTGAAGCAT TCCTGAATGC CGAAGAAGTC 
GACTCCAAGG GCGACAACGT GGAAGCATTG ATTAAGAAGC ACGAAGACTT TGACAAAGCT 
ATCAACGGTC ATGAGGAGAA AATTGCCGCC CTGCAGGTGC TGGCTGATCA ATTGATTGCC 
CAGGAGCACT ACGCAGGAAA ATTAATCGAC GACAAGCGTG CAGATGTGTT GGATCGTTGG 
CGTCACTTGA AGGAAGATTT GATTGAAAAA CGATCTCGCT TGGGAGATGA GCAAACTCTA 
CAGCAATTCT CACGTGATGC TGATGAAATC GAAAACTGGA TTGCCGAGAA GCTGCAACTA 
GCCACGGAGG AAAGTTACAA GGACCCGGCC AATATTCAAT CCAAGCATCA GAAGCACCAG 
GCTTTCGAAG CCGAATTGGC TGCTAATGCC GATCGTATTC AGAGCGTGTT GGCGATGGGA 
AGCAATTTGA TCGATAAGAA TCAATGCAGT GGGTCCGAAG ATGCAGTTCA GAAGCGTTTG 
ACACAGATCG CTGATCAGTG GGAATATCTG ACCCAGAAAA CAACGGAGAA ATCCCTGAAG 
CTGAAGGAAG CTAACAAACA GCGCACTTAT ATTGCTGCTG TCAAGGATTT GGACTTCTGG 
TTGGGTGAAG TTGAGAGTCT TTTGACCTCA GAAGATGCTG GAAAAGATTT GGCTTCCGTC 
CAAAATCTTA TGAAGAAGCA CCAGCTGGTA GAGGCTGACA TCCATGCCCA TGAAGATCGC 
ATCAAGGATA TGAACGCCCA AGCCGACTCA TTGGTCGAAA GTGGCCAATT TGACAGTGCA 
GGAATTCAAG AAAAGCGTCA GTCCATCAAC GAGCGTTACG AACGTATTTG TAATTTGGCA 
GCACATCGCC AAGCTCGCTT GAACGAAGCC AACACTTTGC ATCAATTCTT CCGTGACATT 
GCCGACGAGG AATCCTGGAT TAAGGAAAAG AAATTGTTGG TTGGTTCGGA TGATTATGGT 
CGCGATTTAA CCGGTGTCCA AAATCTGAAA AAGAAGCACA AGCGTCTTGA GGCTGAGTTG 
GCTTCTCACG AGCCAGCAAT TCAGGCTGTT CAGGAAGCTG GTGAAAAGTT GATGGATGTT 
TCGAATCTTG GTGTTCCAGA AATTGAGCAA CGTCTGAAGG CTCTGAACCA AGCCTGGGCT 
GAATTGAAGA GCTTGGCTGC TACTCGTGGT CAGAAACTGG ATGAATCTCT CATTTATCAA 
CAATTCTTAG CTAAGGTTGA AGAAGAGGAA GCATGGATTA CCGAGAAGCA ACAACTGTTG 
TCGGTTGAAG ATTATGGAGA TTCGATGGCT GCTGTCCAGG GTCTTTTGAA GAAACATGAC 
GCCTTCGAGA CCGATTTCGC CGCTCATCGT GATCGTTGCG CTGACATTCA TGACAATGGT 
CAAACTTTGG TGACCAATAA TAATCATCAC GCCGAAAGCA TTTCGCAGCG CTGCACACAA 
CTGGATAAGA AGTTGGAGAA TCTGCAGGCG TTGGCTACTC GTCGCAAGAG CGCCCTTCTG 
GACAACTTTG CTTATCTTCA ATTCATGTGG AAGGCCGATG TGGTTGAAAG TTGGATTGCC 
GATAAAGAAA ATCACGTCAA ATCGGAAGAG TTTGGACGTG ATTTGTCTAC TGTTCAAACA 
TTGCTAACCA AACAGGAGAC ATTCGACGCT GGTCTCTCTG CATTCGAACA TGAAGGAATC 
CATAATATCA CTGCGTTGAA AGATCAACTA ATTAACGCCA ATCACGCCCA ATCGGCCGCC 
ATCCTCAAGC GCCACGAAGA TGTGTTGACC CGCTGGCAGA AATTGCGCGC TGATTCGGAG 
GCTCGCAAGT ATCGTCTATT GGCCATGCAG GAGCAGTTCC GTCAGATTGA AGATCTCTAT 
CTGACGTTTG CCAAGAAGGC TTCCGCTTTC AACTCGTGGT TCGAAAACGC TGAAGAAGAT 
CTCACCGATC CGGTGCGGTG CAATTCGATT GAGGAAATCA AGGCGCTGCG CGAAGCTCAC 
GCCCAGTTCC AGGCATCGTT GTCATCAGCC CAGTATGACT TCCAAGCATT GGCCGATCTA 
GACCGTAAAA TCAAGAGCTT CAACGTTGGT CCCAATCCGT ACACGTGGTT CACAATGGAA 
GCTCTAGAAG ACACCTGGCG TAATCTGCAA AAGATCATCG AAGAACGTGA TGCTGAATTG 
GCAAAGGAAG TTCACCGTCA AGAAGAGAAC GACAAGCTTC GCAAGGAATT CGCCAAACAT 
GCCAACTTGT TCCACCAGTG GCTTACCGAA ACAAGATACA GCATTCTGGG ATGGGACAAA 
AATGGAACAT CTCTTATGGA CGGATCCGGT TCGTTGGAGG AACAATTTGA AGCGCTTTGT 
CACAAAGCAA ATGAAATTCG CGCTCGTCGT GGAGATCTGA AGAAGATTGA AGAGTTGGGT 
GCCACCTTGG AAGAGCACCT TATTTTGGAT AACCGCTATA CTGAACACTC AACTGTAGGC 
CTTGCACAAC AGTGGGATCA GCTTGACCAA CTTGCCATGC GCATGCAGCA CAACCTGAGA 
CAACAAATCC AAGCCCGCAA TCAATCTGGC GTCTCGGAGG ATTCACTCAA AGAATTCTCA 
ATGATGTTCA AGCACTTCGA CAAGGACAAG AGCGGCAAGC TCAACCACCA AGAATTCAAA 
TCCTGTTTAC GTGCCCTGGG TTACGATTTA CCAATGGTGG AGGAAGGCCA GCCCGACCCG 
GAGTTCGAGG AAATTTTGAA TGTGGTGGAC CCGAACCGCG ATGGACATGT TTCACTCCAG 
GAATACATAG CCTTTATGAT CTCCAAGGAA ACGGAGAACG TACAAAGCTA CGAGGAAATC 
GAAAATGCTT TCCGCGCAAT CACCGCCTCC GATCGTCCTT ACGTCACCAA GGATGAACTT 
TATTCCAACC TCACCAAGGA CATGGCAGAT TACTGCGTAC AGCGGATGAA ACCGTTCAAC 
GACCCGAAAA CGGGCCATCC CATCACCGGT GCCCTCGATT ATGTGGAGTT CACGCGAACT 
CTATTCCAAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5566099 ( XM_001650415.2 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001650465.1 Aedes aegypti spectrin alpha chain (LOC5566099), transcript variant X4, mRNA.

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CloneID	OAe121124
Clone ID Related Accession (Same CDS sequence)	XM_001650415.2
Accession Version	XM_001650415.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	spectrin alpha chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 20, 2017 this sequence version replaced XM_001650415.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAGT TTACCCCCAA GGAGGTAAAA ATCCTCGAAA GCGCTGAGGA TATTCAAGAG 
CGCCGTGAAC AGGTGCTCAA CCGATACAAT GAGTTCAAGG TCGAAACGCG CCACAAGCGC 
GAGAAGCTGG AAGATTCGCG TCGTTTCCAG TACTTTAAGC GTGACTCGGA CGAGTTGGAA 
AGCTGGATCC ACGAGAAATT GCAGGCCGCT TCGGAGGAGA GCTATCGCGA TCCTACCAAT 
TTGCAGGCCA AGATTCAGAA GCATCAGGCG TTCGAGGCTG AAGTTTCGGC CCATAGCAAT 
GCGATCGTAG TATTGGACAA CACCGGTCAG GAGATGATCA ATCAGCACCA CTTTGCCTCG 
GACACTATCC AACGTCGCTT GGATGAGTTG CACCGTTTGT GGGAATTGTT GCTGTCCCGT 
TTGGCCGAAA AGGGAATGAA ATTGCAACAG GCTCTGGTCT TGGTGCAATT CTTGCGCCAC 
TGCGAGGAGG TCATGTTCTG GATCAAGGAC AAGGAAGCTT TTGTGACGGC CGATGAGTTC 
GGTCAAGATT TGGAACATGT CGAAGTCTTG CAACGTAAGT TTGACGAGTT CCAGAAGGAC 
ATGGCTTCGC AAGAGTATCG AGTTACTGAA GTCAACGAGT TGGCTGACAA GTTGCTCTCA 
AACGGACACC CTGAGCGTGA AACTATCACT CGCAAGAAGG AAGAATTGAA CGAGGCTTGG 
CAACGCCTGA AGCAGCTGGC TATTCTGCGT CAGGAAAAGC TTTTCGGTGC CCATGAAATT 
CAACGTTTCA ATCGCGATGC TGATGAAACC GTCGCTTGGA TTGCTGAAAA AGACGTTGTT 
CTGTCGTCGG ATGATTATGG ACGTGACTTG GCTAGTGTTC AAGCTTTGCA ACGCAAGCAC 
GAAGGGGTAG AGCGCGACTT GGCTGCTCTG GAAGATAAGG TGGCCACTTT GGGAGCTGAA 
GCCGGTCGCT TATGTAGCAT CCACGCCGAT CATAGTGATC AAATTCGTGA GAAGCAAGCT 
GAAATTGCTG CCTACTGGCA ATCATTGACT GCCAAGGCCA AGGAACGTAA GCAAAAACTG 
GATGAATCAT ATTTCTTGCA CCGTTTCTTG GCTGATTTCC GCGATTTGGT TTCGTGGATC 
AACGGAATGA AGGCCATTAT CTCGGCTGAT GAACTTGCAA AGGATGTAGC TGGAGCTGAG 
GCTCTGCTTG AGCGTCACCA GGAGCACAAA GGAGAAATCG ATGCCCGTGG AGATAGCTTC 
AAGGTTACAA CCGAGGCTGG TCGTCAGCTT TTGGAACGGG AGCATTATGC CGCTGCTGAA 
GTGCAGGAAA AATTGGCTGC TCTAGAGAGC GATAAGAGTT CGCTTCTCGC GCTTTGGGAA 
GAACGTCGCA TTTTGTATGA ACAGTGCATG GATCTACAAC TGTTCTATCG TGACACTGAG 
CAGGCAGATA CTTGGATGGC CAAACAGGAA GCTTTCCTAG CCAATGAAGA TCTAGGAGAT 
TCGCTTGATT CCGTCGAAGC GCTGATTAAG AAGCACGAAG ATTTCGAGAA AAGTTTGGCG 
GCCCAAGAGG AGAAGATCAA GGCTTTGGAT GTTTTCGCTA CAAAGCTCAT CGATGGTCAG 
CATTACGCTG CTGACGATGT TGCCCAACGT CGTTCAATGT TGTTAGCTCG TCGATCAGCC 
TTGCTCGAGA AGTCTTCTGT ACGCCGTCAA CTGCTGGAGG ATTCCAGTGC TTTGCAGCAA 
TTTGAACGTG ACTGCGATGA GACTAAAGGA TGGATCAGCG AAAAGCTTAA ATTTGCCACA 
GATGACAGCT ATTTGGACCC AACCAATCTG AATGGAAAAG TTCAGAAACA TACCAACTTT 
GAGCATGAAT TAACGGCCAA CAAGAGCCGC ATTGAAGACA TCACCACCAA TGGTCAGAAT 
TTAGTCGAGA AGGGACACTA CGGTGCCGAT CAAATCAACT CGCGCATGCA GGAGATTGTC 
ACTTTGTGGG AATCTTTGGT TCAATCATCG GACAAAAAGG GCTGCAAATT GCAGGAAGCA 
TCCCAACAGC AACAATTCAA TCGCACCGTT GAAGATATTG AACTGTGGTT GAGCGAAGTG 
GAAGGCCAAC TAATGTCCGA GGATTATGGC AAAGACCTGA CCAGTGTCCA GAATTTGCAG 
AAGAAACATG GTTTGTTGGA GGCTGATGTC ATGGCCCATC AAGATCGCAT TGAAGGAATT 
AAAGTTGCAG CGAACAAGTT CGTCGAGAGT GGTCATTTCG ATGCCGACAA CATCCGTAAT 
AAGGAATCTG CGCTGTCCAA GCGTTATGGA GCTCTGGCTA CTCCGATGGC TGAACGAAAG 
CAGCGTTTGT TGGATTCCCT ACAGGTGCAG CAACTGTTCC GTGATCTGGA AGACGAAGCT 
GCATGGATCC GTGAGAAGGA ACCTGTCGCT GCATCCACCA ACCGTGGCCG CGATTTAATC 
GGTGTTCAGA ATTTGATTAA GAAGCATCAG GCCGTGTTGG CAGAAATCAA CAATCATGAA 
AATCGCGTCG CCGGAGTCAT CGCGAATGGC GAGCAAATGT TGACTGAGCA GCCTTTTGCC 
ACCGAAGATA TCAAATTGCG TTTGGATGCT GTTAAAGATC AATGGAATTC ATTGAAGGAG 
AAGGCTAATC AACGGAAGCA AGATCTGGAA GATTCGTTGC AGGCCCACCA GTACTTTGCT 
GATGCTAACG AGGCGGAATC TTGGATGCGT GAGAAGGAAC CTATCGTATC GAATCAAGAT 
TACGGCAAGG ACGAAGATTC GTCTGAAGCT TTGTTGAAGA AGCATGAAGC ATTGGTGTCC 
GATTTGGAAG CTTTTGGTAA CACCATTCAA GCTTTGCAAG AACAGGCCAA GAATTGTCGT 
CAGCAGGAAA CACCAGTGGT GGACATTACC GGAAAGGAAT GCGTCATGGC TCTGTATGAT 
TATACCGAGA AATCTCCTCG TGAAGTTTCG ATGAAGAAGG GGGACGTATT AACTCTTTTG 
AACTCGAACA ACAAGGATTG GTGGAAGGTA GAGGTCAACG ATCGTCAGGG ATTCGTTCCG 
GCTGCATACA TTAAGAAGAT CGATCCTGGC CTAAGCGCCA GTCAACAGAA TTTGGTTGAT 
GGCCATTCAA TTGCTAAGCG CCAGGCTCAA ATCAACAGCC AGTACGATAA CTTGCTTGCT 
TTGGCACGTG AACGACAGAA CAAATTGAAT GAAACCGTAA AGGCTTATGT TCTGGTACGT 
GAAGCTGCTG ATTTGTCCGC TTGGATTCGC GACAAGGAAA GCCACGCTCA GATTAAGGAT 
GTTGGCGAAG ATCTGGAAGA AGTTGAAGTT ATGCAGAAGA AGTTTGATGA CTTCAATGAT 
GATCTGAAGG CTAACGAAGT TCGTCTAGCT AAGTTGAACG AGATTGCCGT ACAGCTGACT 
TCGCTAGGTC AAACCGAAGC AGCATTGAAG ATCAAAACTC AAATTCAGAC CTTGAACGAA 
GAGTGGACAG TTTTGCAGAA TATTACCCAG GAAAGGGCTA GTCAACTTGG ATCGGCTCAT 
GAGGTTCAAC GCTTCCATCG TGATGTAGAT GAAACCAAGG ATTGGATTGG CGAGAAGGAC 
ACAGCTTTGA CGAATGACGA TCTTGGTAAG GATCTGCGTG GCGTTCAGAC CCTGCAACGC 
AAGCATGAAG GTTTGGAGCG TGATTTGGCA GCTCTACGAG ACAAAATTCG TCAATTGGAC 
GAAACTGCCA ATCGCCTCAT GCAATCCCAC CCGGAAACCG CCGAGCAGAC TTATGCCAAG 
CAGAAGGAAA TCAACGAGGA ATGGCAGCAG GTTGTTGCCA AAACTCAGCA ACGCAAGGAG 
AAGCTGTTAG ATTCTTATGA TCTACAGCGG TTCCTGAGCG ATTATCGCGA TCTGATGGCT 
TGGATCAGCT CAATGATGGG CTTGGTCACG TCCGAAGAGT TGGCCAACGA TGTCACCGGG 
GCTGAAGCTT TAATCGAAAG ACATCAGGAA CACCGTACCG AGGTAGATGC CCGCGCTGGA 
ACCTTCGCTG CGTTCGATCA GTTTGGAGCT GAGCTGTTGC AGGCAAATCA TTATGCGTCA 
CCCGAAATTC AAGAGAAAAT TGAAAACTTG GCCAAGGCAC GTGAGGATTT GGAGCGTGCC 
TGGACTGCTC GTCGTCTGCA GTTGGACCAG AATCTGGATC TTCAGCTGTA TCTGCGTGAT 
TGCGAACAGG CTGAGAACTG GATGAGCGCC CGTGAAGCAT TCCTGAATGC CGAAGAAGTC 
GACTCCAAGG GCGACAACGT GGAAGCATTG ATTAAGAAGC ACGAAGACTT TGACAAAGCT 
ATCAACGGTC ATGAGGAGAA AATTGCCGCC CTGCAGGTGC TGGCTGATCA ATTGATTGCC 
CAGGAGCACT ACGCAGGAAA ATTAATCGAC GACAAGCGTG CAGATGTGTT GGATCGTTGG 
CGTCACTTGA AGGAAGATTT GATTGAAAAA CGATCTCGCT TGGGAGATGA GCAAACTCTA 
CAGCAATTCT CACGTGATGC TGATGAAATC GAAAACTGGA TTGCCGAGAA GCTGCAACTA 
GCCACGGAGG AAAGTTACAA GGACCCGGCC AATATTCAAT CCAAGCATCA GAAGCACCAG 
GCTTTCGAAG CCGAATTGGC TGCTAATGCC GATCGTATTC AGAGCGTGTT GGCGATGGGA 
AGCAATTTGA TCGATAAGAA TCAATGCAGT GGGTCCGAAG ATGCAGTTCA GAAGCGTTTG 
ACACAGATCG CTGATCAGTG GGAATATCTG ACCCAGAAAA CAACGGAGAA ATCCCTGAAG 
CTGAAGGAAG CTAACAAACA GCGCACTTAT ATTGCTGCTG TCAAGGATTT GGACTTCTGG 
TTGGGTGAAG TTGAGAGTCT TTTGACCTCA GAAGATGCTG GAAAAGATTT GGCTTCCGTC 
CAAAATCTTA TGAAGAAGCA CCAGCTGGTA GAGGCTGACA TCCATGCCCA TGAAGATCGC 
ATCAAGGATA TGAACGCCCA AGCCGACTCA TTGGTCGAAA GTGGCCAATT TGACAGTGCA 
GGAATTCAAG AAAAGCGTCA GTCCATCAAC GAGCGTTACG AACGTATTTG TAATTTGGCA 
GCACATCGCC AAGCTCGCTT GAACGAAGCC AACACTTTGC ATCAATTCTT CCGTGACATT 
GCCGACGAGG AATCCTGGAT TAAGGAAAAG AAATTGTTGG TTGGTTCGGA TGATTATGGT 
CGCGATTTAA CCGGTGTCCA AAATCTGAAA AAGAAGCACA AGCGTCTTGA GGCTGAGTTG 
GCTTCTCACG AGCCAGCAAT TCAGGCTGTT CAGGAAGCTG GTGAAAAGTT GATGGATGTT 
TCGAATCTTG GTGTTCCAGA AATTGAGCAA CGTCTGAAGG CTCTGAACCA AGCCTGGGCT 
GAATTGAAGA GCTTGGCTGC TACTCGTGGT CAGAAACTGG ATGAATCTCT CATTTATCAA 
CAATTCTTAG CTAAGGTTGA AGAAGAGGAA GCATGGATTA CCGAGAAGCA ACAACTGTTG 
TCGGTTGAAG ATTATGGAGA TTCGATGGCT GCTGTCCAGG GTCTTTTGAA GAAACATGAC 
GCCTTCGAGA CCGATTTCGC CGCTCATCGT GATCGTTGCG CTGACATTCA TGACAATGGT 
CAAACTTTGG TGACCAATAA TAATCATCAC GCCGAAAGCA TTTCGCAGCG CTGCACACAA 
CTGGATAAGA AGTTGGAGAA TCTGCAGGCG TTGGCTACTC GTCGCAAGAG CGCCCTTCTG 
GACAACTTTG CTTATCTTCA ATTCATGTGG AAGGCCGATG TGGTTGAAAG TTGGATTGCC 
GATAAAGAAA ATCACGTCAA ATCGGAAGAG TTTGGACGTG ATTTGTCTAC TGTTCAAACA 
TTGCTAACCA AACAGGAGAC ATTCGACGCT GGTCTCTCTG CATTCGAACA TGAAGGAATC 
CATAATATCA CTGCGTTGAA AGATCAACTA ATTAACGCCA ATCACGCCCA ATCGGCCGCC 
ATCCTCAAGC GCCACGAAGA TGTGTTGACC CGCTGGCAGA AATTGCGCGC TGATTCGGAG 
GCTCGCAAGT ATCGTCTATT GGCCATGCAG GAGCAGTTCC GTCAGATTGA AGATCTCTAT 
CTGACGTTTG CCAAGAAGGC TTCCGCTTTC AACTCGTGGT TCGAAAACGC TGAAGAAGAT 
CTCACCGATC CGGTGCGGTG CAATTCGATT GAGGAAATCA AGGCGCTGCG CGAAGCTCAC 
GCCCAGTTCC AGGCATCGTT GTCATCAGCC CAGTATGACT TCCAAGCATT GGCCGATCTA 
GACCGTAAAA TCAAGAGCTT CAACGTTGGT CCCAATCCGT ACACGTGGTT CACAATGGAA 
GCTCTAGAAG ACACCTGGCG TAATCTGCAA AAGATCATCG AAGAACGTGA TGCTGAATTG 
GCAAAGGAAG TTCACCGTCA AGAAGAGAAC GACAAGCTTC GCAAGGAATT CGCCAAACAT 
GCCAACTTGT TCCACCAGTG GCTTACCGAA ACAAGAACAT CTCTTATGGA CGGATCCGGT 
TCGTTGGAGG AACAATTTGA AGCGCTTTGT CACAAAGCAA ATGAAATTCG CGCTCGTCGT 
GGAGATCTGA AGAAGATTGA AGAGTTGGGT GCCACCTTGG AAGAGCACCT TATTTTGGAT 
AACCGCTATA CTGAACACTC AACTGTAGGC CTTGCACAAC AGTGGGATCA GCTTGACCAA 
CTTGCCATGC GCATGCAGCA CAACCTGAGA CAACAAATCC AAGCCCGCAA TCAATCTGGC 
GTCTCGGAGG ATTCACTCAA AGAATTCTCA ATGATGTTCA AGCACTTCGA CAAGGACAAG 
AGCGGCAAGC TCAACCACCA AGAATTCAAA TCCTGTTTAC GTGCCCTGGG TTACGATTTA 
CCAATGGTGG AGGAAGGCCA GCCCGACCCG GAGTTCGAGG AAATTTTGAA TGTGGTGGAC 
CCGAACCGCG ATGGACATGT TTCACTCCAG GAATACATAG CCTTTATGAT CTCCAAGGAA 
ACGGAGAACG TACAAAGCTA CGAGGAAATC GAAAATGCTT TCCGCGCAAT CACCGCCTCC 
GATCGTCCTT ACGTCACCAA GGATGAACTT TATTCCAACC TCACCAAGGA CATGGCAGAT 
TACTGCGTAC AGCGGATGAA ACCGTTCAAC GACCCGAAAA CGGGCCATCC CATCACCGGT 
GCCCTCGATT ATGTGGAGTT CACGCGAACT CTATTCCAAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001650465.1
CDS	273..7517
Translation	MEQFTPKEVKILESAEDIQERREQVLNRYNEFKVETRHKREKLEDSRRFQYFKRDSDELESWIHEKLQAASEESYRDPTNLQAKIQKHQAFEAEVSAHSNAIVVLDNTGQEMINQHHFASDTIQRRLDELHRLWELLLSRLAEKGMKLQQALVLVQFLRHCEEVMFWIKDKEAFVTADEFGQDLEHVEVLQRKFDEFQKDMASQEYRVTEVNELADKLLSNGHPERETITRKKEELNEAWQRLKQLAILRQEKLFGAHEIQRFNRDADETVAWIAEKDVVLSSDDYGRDLASVQALQRKHEGVERDLAALEDKVATLGAEAGRLCSIHADHSDQIREKQAEIAAYWQSLTAKAKERKQKLDESYFLHRFLADFRDLVSWINGMKAIISADELAKDVAGAEALLERHQEHKGEIDARGDSFKVTTEAGRQLLEREHYAAAEVQEKLAALESDKSSLLALWEERRILYEQCMDLQLFYRDTEQADTWMAKQEAFLANEDLGDSLDSVEALIKKHEDFEKSLAAQEEKIKALDVFATKLIDGQHYAADDVAQRRSMLLARRSALLEKSSVRRQLLEDSSALQQFERDCDETKGWISEKLKFATDDSYLDPTNLNGKVQKHTNFEHELTANKSRIEDITTNGQNLVEKGHYGADQINSRMQEIVTLWESLVQSSDKKGCKLQEASQQQQFNRTVEDIELWLSEVEGQLMSEDYGKDLTSVQNLQKKHGLLEADVMAHQDRIEGIKVAANKFVESGHFDADNIRNKESALSKRYGALATPMAERKQRLLDSLQVQQLFRDLEDEAAWIREKEPVAASTNRGRDLIGVQNLIKKHQAVLAEINNHENRVAGVIANGEQMLTEQPFATEDIKLRLDAVKDQWNSLKEKANQRKQDLEDSLQAHQYFADANEAESWMREKEPIVSNQDYGKDEDSSEALLKKHEALVSDLEAFGNTIQALQEQAKNCRQQETPVVDITGKECVMALYDYTEKSPREVSMKKGDVLTLLNSNNKDWWKVEVNDRQGFVPAAYIKKIDPGLSASQQNLVDGHSIAKRQAQINSQYDNLLALARERQNKLNETVKAYVLVREAADLSAWIRDKESHAQIKDVGEDLEEVEVMQKKFDDFNDDLKANEVRLAKLNEIAVQLTSLGQTEAALKIKTQIQTLNEEWTVLQNITQERASQLGSAHEVQRFHRDVDETKDWIGEKDTALTNDDLGKDLRGVQTLQRKHEGLERDLAALRDKIRQLDETANRLMQSHPETAEQTYAKQKEINEEWQQVVAKTQQRKEKLLDSYDLQRFLSDYRDLMAWISSMMGLVTSEELANDVTGAEALIERHQEHRTEVDARAGTFAAFDQFGAELLQANHYASPEIQEKIENLAKAREDLERAWTARRLQLDQNLDLQLYLRDCEQAENWMSAREAFLNAEEVDSKGDNVEALIKKHEDFDKAINGHEEKIAALQVLADQLIAQEHYAGKLIDDKRADVLDRWRHLKEDLIEKRSRLGDEQTLQQFSRDADEIENWIAEKLQLATEESYKDPANIQSKHQKHQAFEAELAANADRIQSVLAMGSNLIDKNQCSGSEDAVQKRLTQIADQWEYLTQKTTEKSLKLKEANKQRTYIAAVKDLDFWLGEVESLLTSEDAGKDLASVQNLMKKHQLVEADIHAHEDRIKDMNAQADSLVESGQFDSAGIQEKRQSINERYERICNLAAHRQARLNEANTLHQFFRDIADEESWIKEKKLLVGSDDYGRDLTGVQNLKKKHKRLEAELASHEPAIQAVQEAGEKLMDVSNLGVPEIEQRLKALNQAWAELKSLAATRGQKLDESLIYQQFLAKVEEEEAWITEKQQLLSVEDYGDSMAAVQGLLKKHDAFETDFAAHRDRCADIHDNGQTLVTNNNHHAESISQRCTQLDKKLENLQALATRRKSALLDNFAYLQFMWKADVVESWIADKENHVKSEEFGRDLSTVQTLLTKQETFDAGLSAFEHEGIHNITALKDQLINANHAQSAAILKRHEDVLTRWQKLRADSEARKYRLLAMQEQFRQIEDLYLTFAKKASAFNSWFENAEEDLTDPVRCNSIEEIKALREAHAQFQASLSSAQYDFQALADLDRKIKSFNVGPNPYTWFTMEALEDTWRNLQKIIEERDAELAKEVHRQEENDKLRKEFAKHANLFHQWLTETRTSLMDGSGSLEEQFEALCHKANEIRARRGDLKKIEELGATLEEHLILDNRYTEHSTVGLAQQWDQLDQLAMRMQHNLRQQIQARNQSGVSEDSLKEFSMMFKHFDKDKSGKLNHQEFKSCLRALGYDLPMVEEGQPDPEFEEILNVVDPNRDGHVSLQEYIAFMISKETENVQSYEEIENAFRAITASDRPYVTKDELYSNLTKDMADYCVQRMKPFNDPKTGHPITGALDYVEFTRTLFQN

Target ORF information:

RefSeq Version	XM_001650415.2
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti spectrin alpha chain (LOC5566099), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001650415.2

ORF Insert Sequence:

ATGGAACAGT TTACCCCCAA GGAGGTAAAA ATCCTCGAAA GCGCTGAGGA TATTCAAGAG 
CGCCGTGAAC AGGTGCTCAA CCGATACAAT GAGTTCAAGG TCGAAACGCG CCACAAGCGC 
GAGAAGCTGG AAGATTCGCG TCGTTTCCAG TACTTTAAGC GTGACTCGGA CGAGTTGGAA 
AGCTGGATCC ACGAGAAATT GCAGGCCGCT TCGGAGGAGA GCTATCGCGA TCCTACCAAT 
TTGCAGGCCA AGATTCAGAA GCATCAGGCG TTCGAGGCTG AAGTTTCGGC CCATAGCAAT 
GCGATCGTAG TATTGGACAA CACCGGTCAG GAGATGATCA ATCAGCACCA CTTTGCCTCG 
GACACTATCC AACGTCGCTT GGATGAGTTG CACCGTTTGT GGGAATTGTT GCTGTCCCGT 
TTGGCCGAAA AGGGAATGAA ATTGCAACAG GCTCTGGTCT TGGTGCAATT CTTGCGCCAC 
TGCGAGGAGG TCATGTTCTG GATCAAGGAC AAGGAAGCTT TTGTGACGGC CGATGAGTTC 
GGTCAAGATT TGGAACATGT CGAAGTCTTG CAACGTAAGT TTGACGAGTT CCAGAAGGAC 
ATGGCTTCGC AAGAGTATCG AGTTACTGAA GTCAACGAGT TGGCTGACAA GTTGCTCTCA 
AACGGACACC CTGAGCGTGA AACTATCACT CGCAAGAAGG AAGAATTGAA CGAGGCTTGG 
CAACGCCTGA AGCAGCTGGC TATTCTGCGT CAGGAAAAGC TTTTCGGTGC CCATGAAATT 
CAACGTTTCA ATCGCGATGC TGATGAAACC GTCGCTTGGA TTGCTGAAAA AGACGTTGTT 
CTGTCGTCGG ATGATTATGG ACGTGACTTG GCTAGTGTTC AAGCTTTGCA ACGCAAGCAC 
GAAGGGGTAG AGCGCGACTT GGCTGCTCTG GAAGATAAGG TGGCCACTTT GGGAGCTGAA 
GCCGGTCGCT TATGTAGCAT CCACGCCGAT CATAGTGATC AAATTCGTGA GAAGCAAGCT 
GAAATTGCTG CCTACTGGCA ATCATTGACT GCCAAGGCCA AGGAACGTAA GCAAAAACTG 
GATGAATCAT ATTTCTTGCA CCGTTTCTTG GCTGATTTCC GCGATTTGGT TTCGTGGATC 
AACGGAATGA AGGCCATTAT CTCGGCTGAT GAACTTGCAA AGGATGTAGC TGGAGCTGAG 
GCTCTGCTTG AGCGTCACCA GGAGCACAAA GGAGAAATCG ATGCCCGTGG AGATAGCTTC 
AAGGTTACAA CCGAGGCTGG TCGTCAGCTT TTGGAACGGG AGCATTATGC CGCTGCTGAA 
GTGCAGGAAA AATTGGCTGC TCTAGAGAGC GATAAGAGTT CGCTTCTCGC GCTTTGGGAA 
GAACGTCGCA TTTTGTATGA ACAGTGCATG GATCTACAAC TGTTCTATCG TGACACTGAG 
CAGGCAGATA CTTGGATGGC CAAACAGGAA GCTTTCCTAG CCAATGAAGA TCTAGGAGAT 
TCGCTTGATT CCGTCGAAGC GCTGATTAAG AAGCACGAAG ATTTCGAGAA AAGTTTGGCG 
GCCCAAGAGG AGAAGATCAA GGCTTTGGAT GTTTTCGCTA CAAAGCTCAT CGATGGTCAG 
CATTACGCTG CTGACGATGT TGCCCAACGT CGTTCAATGT TGTTAGCTCG TCGATCAGCC 
TTGCTCGAGA AGTCTTCTGT ACGCCGTCAA CTGCTGGAGG ATTCCAGTGC TTTGCAGCAA 
TTTGAACGTG ACTGCGATGA GACTAAAGGA TGGATCAGCG AAAAGCTTAA ATTTGCCACA 
GATGACAGCT ATTTGGACCC AACCAATCTG AATGGAAAAG TTCAGAAACA TACCAACTTT 
GAGCATGAAT TAACGGCCAA CAAGAGCCGC ATTGAAGACA TCACCACCAA TGGTCAGAAT 
TTAGTCGAGA AGGGACACTA CGGTGCCGAT CAAATCAACT CGCGCATGCA GGAGATTGTC 
ACTTTGTGGG AATCTTTGGT TCAATCATCG GACAAAAAGG GCTGCAAATT GCAGGAAGCA 
TCCCAACAGC AACAATTCAA TCGCACCGTT GAAGATATTG AACTGTGGTT GAGCGAAGTG 
GAAGGCCAAC TAATGTCCGA GGATTATGGC AAAGACCTGA CCAGTGTCCA GAATTTGCAG 
AAGAAACATG GTTTGTTGGA GGCTGATGTC ATGGCCCATC AAGATCGCAT TGAAGGAATT 
AAAGTTGCAG CGAACAAGTT CGTCGAGAGT GGTCATTTCG ATGCCGACAA CATCCGTAAT 
AAGGAATCTG CGCTGTCCAA GCGTTATGGA GCTCTGGCTA CTCCGATGGC TGAACGAAAG 
CAGCGTTTGT TGGATTCCCT ACAGGTGCAG CAACTGTTCC GTGATCTGGA AGACGAAGCT 
GCATGGATCC GTGAGAAGGA ACCTGTCGCT GCATCCACCA ACCGTGGCCG CGATTTAATC 
GGTGTTCAGA ATTTGATTAA GAAGCATCAG GCCGTGTTGG CAGAAATCAA CAATCATGAA 
AATCGCGTCG CCGGAGTCAT CGCGAATGGC GAGCAAATGT TGACTGAGCA GCCTTTTGCC 
ACCGAAGATA TCAAATTGCG TTTGGATGCT GTTAAAGATC AATGGAATTC ATTGAAGGAG 
AAGGCTAATC AACGGAAGCA AGATCTGGAA GATTCGTTGC AGGCCCACCA GTACTTTGCT 
GATGCTAACG AGGCGGAATC TTGGATGCGT GAGAAGGAAC CTATCGTATC GAATCAAGAT 
TACGGCAAGG ACGAAGATTC GTCTGAAGCT TTGTTGAAGA AGCATGAAGC ATTGGTGTCC 
GATTTGGAAG CTTTTGGTAA CACCATTCAA GCTTTGCAAG AACAGGCCAA GAATTGTCGT 
CAGCAGGAAA CACCAGTGGT GGACATTACC GGAAAGGAAT GCGTCATGGC TCTGTATGAT 
TATACCGAGA AATCTCCTCG TGAAGTTTCG ATGAAGAAGG GGGACGTATT AACTCTTTTG 
AACTCGAACA ACAAGGATTG GTGGAAGGTA GAGGTCAACG ATCGTCAGGG ATTCGTTCCG 
GCTGCATACA TTAAGAAGAT CGATCCTGGC CTAAGCGCCA GTCAACAGAA TTTGGTTGAT 
GGCCATTCAA TTGCTAAGCG CCAGGCTCAA ATCAACAGCC AGTACGATAA CTTGCTTGCT 
TTGGCACGTG AACGACAGAA CAAATTGAAT GAAACCGTAA AGGCTTATGT TCTGGTACGT 
GAAGCTGCTG ATTTGTCCGC TTGGATTCGC GACAAGGAAA GCCACGCTCA GATTAAGGAT 
GTTGGCGAAG ATCTGGAAGA AGTTGAAGTT ATGCAGAAGA AGTTTGATGA CTTCAATGAT 
GATCTGAAGG CTAACGAAGT TCGTCTAGCT AAGTTGAACG AGATTGCCGT ACAGCTGACT 
TCGCTAGGTC AAACCGAAGC AGCATTGAAG ATCAAAACTC AAATTCAGAC CTTGAACGAA 
GAGTGGACAG TTTTGCAGAA TATTACCCAG GAAAGGGCTA GTCAACTTGG ATCGGCTCAT 
GAGGTTCAAC GCTTCCATCG TGATGTAGAT GAAACCAAGG ATTGGATTGG CGAGAAGGAC 
ACAGCTTTGA CGAATGACGA TCTTGGTAAG GATCTGCGTG GCGTTCAGAC CCTGCAACGC 
AAGCATGAAG GTTTGGAGCG TGATTTGGCA GCTCTACGAG ACAAAATTCG TCAATTGGAC 
GAAACTGCCA ATCGCCTCAT GCAATCCCAC CCGGAAACCG CCGAGCAGAC TTATGCCAAG 
CAGAAGGAAA TCAACGAGGA ATGGCAGCAG GTTGTTGCCA AAACTCAGCA ACGCAAGGAG 
AAGCTGTTAG ATTCTTATGA TCTACAGCGG TTCCTGAGCG ATTATCGCGA TCTGATGGCT 
TGGATCAGCT CAATGATGGG CTTGGTCACG TCCGAAGAGT TGGCCAACGA TGTCACCGGG 
GCTGAAGCTT TAATCGAAAG ACATCAGGAA CACCGTACCG AGGTAGATGC CCGCGCTGGA 
ACCTTCGCTG CGTTCGATCA GTTTGGAGCT GAGCTGTTGC AGGCAAATCA TTATGCGTCA 
CCCGAAATTC AAGAGAAAAT TGAAAACTTG GCCAAGGCAC GTGAGGATTT GGAGCGTGCC 
TGGACTGCTC GTCGTCTGCA GTTGGACCAG AATCTGGATC TTCAGCTGTA TCTGCGTGAT 
TGCGAACAGG CTGAGAACTG GATGAGCGCC CGTGAAGCAT TCCTGAATGC CGAAGAAGTC 
GACTCCAAGG GCGACAACGT GGAAGCATTG ATTAAGAAGC ACGAAGACTT TGACAAAGCT 
ATCAACGGTC ATGAGGAGAA AATTGCCGCC CTGCAGGTGC TGGCTGATCA ATTGATTGCC 
CAGGAGCACT ACGCAGGAAA ATTAATCGAC GACAAGCGTG CAGATGTGTT GGATCGTTGG 
CGTCACTTGA AGGAAGATTT GATTGAAAAA CGATCTCGCT TGGGAGATGA GCAAACTCTA 
CAGCAATTCT CACGTGATGC TGATGAAATC GAAAACTGGA TTGCCGAGAA GCTGCAACTA 
GCCACGGAGG AAAGTTACAA GGACCCGGCC AATATTCAAT CCAAGCATCA GAAGCACCAG 
GCTTTCGAAG CCGAATTGGC TGCTAATGCC GATCGTATTC AGAGCGTGTT GGCGATGGGA 
AGCAATTTGA TCGATAAGAA TCAATGCAGT GGGTCCGAAG ATGCAGTTCA GAAGCGTTTG 
ACACAGATCG CTGATCAGTG GGAATATCTG ACCCAGAAAA CAACGGAGAA ATCCCTGAAG 
CTGAAGGAAG CTAACAAACA GCGCACTTAT ATTGCTGCTG TCAAGGATTT GGACTTCTGG 
TTGGGTGAAG TTGAGAGTCT TTTGACCTCA GAAGATGCTG GAAAAGATTT GGCTTCCGTC 
CAAAATCTTA TGAAGAAGCA CCAGCTGGTA GAGGCTGACA TCCATGCCCA TGAAGATCGC 
ATCAAGGATA TGAACGCCCA AGCCGACTCA TTGGTCGAAA GTGGCCAATT TGACAGTGCA 
GGAATTCAAG AAAAGCGTCA GTCCATCAAC GAGCGTTACG AACGTATTTG TAATTTGGCA 
GCACATCGCC AAGCTCGCTT GAACGAAGCC AACACTTTGC ATCAATTCTT CCGTGACATT 
GCCGACGAGG AATCCTGGAT TAAGGAAAAG AAATTGTTGG TTGGTTCGGA TGATTATGGT 
CGCGATTTAA CCGGTGTCCA AAATCTGAAA AAGAAGCACA AGCGTCTTGA GGCTGAGTTG 
GCTTCTCACG AGCCAGCAAT TCAGGCTGTT CAGGAAGCTG GTGAAAAGTT GATGGATGTT 
TCGAATCTTG GTGTTCCAGA AATTGAGCAA CGTCTGAAGG CTCTGAACCA AGCCTGGGCT 
GAATTGAAGA GCTTGGCTGC TACTCGTGGT CAGAAACTGG ATGAATCTCT CATTTATCAA 
CAATTCTTAG CTAAGGTTGA AGAAGAGGAA GCATGGATTA CCGAGAAGCA ACAACTGTTG 
TCGGTTGAAG ATTATGGAGA TTCGATGGCT GCTGTCCAGG GTCTTTTGAA GAAACATGAC 
GCCTTCGAGA CCGATTTCGC CGCTCATCGT GATCGTTGCG CTGACATTCA TGACAATGGT 
CAAACTTTGG TGACCAATAA TAATCATCAC GCCGAAAGCA TTTCGCAGCG CTGCACACAA 
CTGGATAAGA AGTTGGAGAA TCTGCAGGCG TTGGCTACTC GTCGCAAGAG CGCCCTTCTG 
GACAACTTTG CTTATCTTCA ATTCATGTGG AAGGCCGATG TGGTTGAAAG TTGGATTGCC 
GATAAAGAAA ATCACGTCAA ATCGGAAGAG TTTGGACGTG ATTTGTCTAC TGTTCAAACA 
TTGCTAACCA AACAGGAGAC ATTCGACGCT GGTCTCTCTG CATTCGAACA TGAAGGAATC 
CATAATATCA CTGCGTTGAA AGATCAACTA ATTAACGCCA ATCACGCCCA ATCGGCCGCC 
ATCCTCAAGC GCCACGAAGA TGTGTTGACC CGCTGGCAGA AATTGCGCGC TGATTCGGAG 
GCTCGCAAGT ATCGTCTATT GGCCATGCAG GAGCAGTTCC GTCAGATTGA AGATCTCTAT 
CTGACGTTTG CCAAGAAGGC TTCCGCTTTC AACTCGTGGT TCGAAAACGC TGAAGAAGAT 
CTCACCGATC CGGTGCGGTG CAATTCGATT GAGGAAATCA AGGCGCTGCG CGAAGCTCAC 
GCCCAGTTCC AGGCATCGTT GTCATCAGCC CAGTATGACT TCCAAGCATT GGCCGATCTA 
GACCGTAAAA TCAAGAGCTT CAACGTTGGT CCCAATCCGT ACACGTGGTT CACAATGGAA 
GCTCTAGAAG ACACCTGGCG TAATCTGCAA AAGATCATCG AAGAACGTGA TGCTGAATTG 
GCAAAGGAAG TTCACCGTCA AGAAGAGAAC GACAAGCTTC GCAAGGAATT CGCCAAACAT 
GCCAACTTGT TCCACCAGTG GCTTACCGAA ACAAGAACAT CTCTTATGGA CGGATCCGGT 
TCGTTGGAGG AACAATTTGA AGCGCTTTGT CACAAAGCAA ATGAAATTCG CGCTCGTCGT 
GGAGATCTGA AGAAGATTGA AGAGTTGGGT GCCACCTTGG AAGAGCACCT TATTTTGGAT 
AACCGCTATA CTGAACACTC AACTGTAGGC CTTGCACAAC AGTGGGATCA GCTTGACCAA 
CTTGCCATGC GCATGCAGCA CAACCTGAGA CAACAAATCC AAGCCCGCAA TCAATCTGGC 
GTCTCGGAGG ATTCACTCAA AGAATTCTCA ATGATGTTCA AGCACTTCGA CAAGGACAAG 
AGCGGCAAGC TCAACCACCA AGAATTCAAA TCCTGTTTAC GTGCCCTGGG TTACGATTTA 
CCAATGGTGG AGGAAGGCCA GCCCGACCCG GAGTTCGAGG AAATTTTGAA TGTGGTGGAC 
CCGAACCGCG ATGGACATGT TTCACTCCAG GAATACATAG CCTTTATGAT CTCCAAGGAA 
ACGGAGAACG TACAAAGCTA CGAGGAAATC GAAAATGCTT TCCGCGCAAT CACCGCCTCC 
GATCGTCCTT ACGTCACCAA GGATGAACTT TATTCCAACC TCACCAAGGA CATGGCAGAT 
TACTGCGTAC AGCGGATGAA ACCGTTCAAC GACCCGAAAA CGGGCCATCC CATCACCGGT 
GCCCTCGATT ATGTGGAGTT CACGCGAACT CTATTCCAAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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LOC5566099 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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