LOC5564138 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC5564138
Entrez Gene ID	5564138
Full Name	trichohyalin
Synonyms	AAEL004085,AAEL004099
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021855367.1	XP_021711059.1	uncharacterized protein LOC5564138 isoform X1
XM_021855369.1	XP_021711061.1	uncharacterized protein LOC5564138 isoform X3
XM_001648416.1	XP_001648466.1	AAEL004099-PA
XM_021855368.1	XP_021711060.1	interaptin isoform X2
XM_021855370.1	XP_021711062.1	trichohyalin isoform X4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC5564138 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC5564138 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe00511	XM_001648416.1 Latest version!	Aedes aegypti AAEL004099-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAe129904	XM_021855370.1 Latest version!	Aedes aegypti trichohyalin (LOC5564138), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAe129903	XM_021855369.1 Latest version!	Aedes aegypti trichohyalin (LOC5564138), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAe129901	XM_021855367.1 Latest version!	Aedes aegypti trichohyalin (LOC5564138), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAe129902	XM_021855368.1 Latest version!	Aedes aegypti trichohyalin (LOC5564138), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC5564138 ( XM_001648416.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001648466.1 Aedes aegypti AAEL004099-RA partial mRNA.

Price & Availability↑

CloneID	OAe00511
Clone ID Related Accession (Same CDS sequence)	XM_001648416.1
Accession Version	XM_001648416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL004099-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001809879). COMPLETENESS: incomplete on both ends.

ATGAACCCGT CATATTCTAT TCTATTGAAA CGGAAACAGC ACTCAATCAC ACTCCACTTG 
TGCAACGAAC AGCGCGAGGG TGTGCTCCAG TTGTTGCATG ACAGCTTTTA TATTATACCA 
GTTGTCCCGG GTCCAGTATT GAAGGAGCTA GTGAATCAGG AATCCCGCTA CAGCGTCAAG 
CTTGAGGATG CTGAGAAGCG GGCCAGCGAG GCCCAGACTA AGGTGTACGA GCTGAAGCAA 
CGGTTGGATG AGGTGGAAAG AGAAGTTTCC TTGAAAGAAT GCAACGTAGA CCGCCTCAAA 
GCGGAACTAG ATGCAGCGTG CAAGGAATGT GAGACTATCC GTGCGCGGAT GCACTCGCAG 
AGCTCCGAAC TGGACGCACT AAGACTGAAA TTCTCCGAAC GGGAGGATGA GCTAAATCTT 
AAGTACCAAA ATTTAGAAAT AGAATACATA GAGTTAACCG AGAAGCTGAA AGATGTGCGT 
CAGTTGGCCC ACGATTTGAA CATGCAGCTC ATCAACGCCC AATCGGAAGC GGAGCGGGTC 
CAGAAGGAGA AGAATCAGCT CCTGGAGGAA CGTGCCGAGG AGCAGAAACT GGTCAAGGAA 
GCGCTCGAGT CGGCCTTGAA AGAGCGAGCG CAGATCGAGG CCAAGTGGAA GAATGATTTC 
GAGCAGCTGC GGACGGTGCA CTCCGACCGG GAGGAGCAAT TCATGGAGGA CTGCGAGTGG 
AAGATACGGT CGATGCAGAA GAACTGCAAG GAGAAGATGG AGAAGATCGA AAGCGAACAC 
AAGATGGCCA TGGACAAGAT GGCGCAGTTG GAGCAAGAGT CGAGCAAACA TATAGAGGAG 
GCCAAACACC TAAGATCTTA CGAAGCGGAG GTGGCCCAGT TACGAGGGTT GACTTACGAC 
CAAAAGGAGG CACTGAAGAC AATGACGCGC CAGATTGACC AACTCAAGGC CGACTTGCTG 
ATGGCCAATA ACAAACTGGA AGCGGAAATT GTTAAGGTGC AACAGATCAA GAACCGCTGT 
GAATACCAAC TATGTGAAAA GGAGCGTGAG GCTCTCAACC GAATAGAAAT CGCTCGAGGA 
GAGATTGCAA TGCAGTGGGA GGATCGGTTG CTTCACGAGA TGAATCGCCT AAAGATGGAA 
CTGGAGCAGA TGTACATCGA GGAGCGAGTT GCCGCCATCA ACAAGTTCAA ACGGGAAGCC 
CTGGAGGAAA CGGAAGCGCT CAAGCACAAG TTCAACATGC GCGAGAGGCA ACTCAAAGAA 
GAGATTGAAT CACTGAAAAG CACAATCCAG TTGCAAAAAC AAGCGATGGA GGCTGCCCAA 
ACGGAAGCGG ATCACAGACT AGTGCAGTCT CGAATGTTTG TAGAACGAGC AGAACGAGAG 
CATGAGACTA TTTTGGCCAA AGAAGTTTCC AAGAGGGATC AAATTATTGA TAATCTCAAA 
GAACAATACG AGAAGGAAAA GTTCGAGATG GAGCAACACT TTAGTCTGAG AATACAGCAA 
GTACAGGAAG AGTTCCAACG GGAATTGTCC GATACAACCG AATTGCTGAA AGCTACACAC 
AAGAAAGAAT TGGAACAACA ATGGAAGAAT TTGGTGAACG AGAAGGAAGA AGCACTCCAA 
CTGATGGACT CCCGACAAAG AAACCGTCTT CAGGAAGCTG AAAATGAAAT CAGAGAGCTA 
AAGATCGGCC ATCAGCGGGA AATGAAGGAC CTTCACGAGG AACAACTGTT CACGATTACC 
AGTCTGGAGG CACGCGATAC CAAAAATGCC CAGGAAATTC AAACCTTGCA CAAAAAGTGT 
CGATGTTTAA CGAATCTTTT CGAGGAAATG CGAATGCGAT ACGAACGTCG AGATCCTCGG 
CAGGAAGATT TGCAGCAGAT CGAGGAATTG AAGAGTGTGA TCGAGTCCCA GGACCGTGAT 
CTAAGACTCT TAACAGAACA ATTTCGAGAA ATGCAACTGC ACGAGAGGGA ACTGCAGCAA 
AGGTTATATC AACAGCAAAT GCAGCAGCAA CAACCTCCAC AACCTCCGAG GAGAGCGAAG 
AACCGTGGCA AACAGCAACA AAATCAACAG AATGATCAAA TAGTGATAGA AGAAGACGAA 
CAACAACAGC AGCAGCAACA ACAACAGCAT CAACCTCAGA TACAGATCCA ACCTATGACA 
GCATCTACAC CTATAGTTTG TGATGTGATC TACGAAGAGA ATGAAGCTGA TCTAATCCAA 
GAGGAAGAAC AGCAACCAAC ACCTTCTCAT ATTGTACATC AAGATCAAAC AAACGCAAAG 
CAAACACAAA TTGTCGAGCA GCAGGATCCA CAGAGCCAGG AAGAGGTGAT AGTAGACGTT 
CCAGAATCTG CCATCGAAAC TTTGGTGGAC GTTGTGCAGT CGACTGAACC TGTTTTCATC 
GAAAATATCC CAAGTTTAGA TTCAGAACCG AATCAATGTA ATGAACCTGC TGAGGAATCA 
TCCAGTCCTG TTGAACCATC AGAAGTCCCT GTCATACAAA TTAGTTGTCT CCCAGAATCA 
AAGCCTGCAG AAACTGATGT GTCTGAGTCT ATACCAACGC CTGCAATTGT TATCACTACA 
GAAAACGAAG AATCGATAGA AGCAGAAAAT GTTGAAGTAT CTATATCGAC GGTAGTAGAA 
CTTCCTGCGC CTCAACAGTT TATCAAAGTT GTAGAAGATC CATCTCCAGC AAAGCCGGCT 
CTAGTGGAAC CTCAAACCGA AGTGCAACAT AAAGTACAAA GCATGCCGGA AATGATTGTC 
ATAAGAGAAC CGGATGTGAT TGCAGAGGCC AAGGCTGCTG TTGCTGCTAC TGCTGAGGTT 
TCGACAGCTG TTGATGTGGT TGTATGTGCG CCCGGTACGC GATCTTCATC GACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001648466.1
CDS	1..2877
Translation	MNPSYSILLKRKQHSITLHLCNEQREGVLQLLHDSFYIIPVVPGPVLKELVNQESRYSVKLEDAEKRASEAQTKVYELKQRLDEVEREVSLKECNVDRLKAELDAACKECETIRARMHSQSSELDALRLKFSEREDELNLKYQNLEIEYIELTEKLKDVRQLAHDLNMQLINAQSEAERVQKEKNQLLEERAEEQKLVKEALESALKERAQIEAKWKNDFEQLRTVHSDREEQFMEDCEWKIRSMQKNCKEKMEKIESEHKMAMDKMAQLEQESSKHIEEAKHLRSYEAEVAQLRGLTYDQKEALKTMTRQIDQLKADLLMANNKLEAEIVKVQQIKNRCEYQLCEKEREALNRIEIARGEIAMQWEDRLLHEMNRLKMELEQMYIEERVAAINKFKREALEETEALKHKFNMRERQLKEEIESLKSTIQLQKQAMEAAQTEADHRLVQSRMFVERAEREHETILAKEVSKRDQIIDNLKEQYEKEKFEMEQHFSLRIQQVQEEFQRELSDTTELLKATHKKELEQQWKNLVNEKEEALQLMDSRQRNRLQEAENEIRELKIGHQREMKDLHEEQLFTITSLEARDTKNAQEIQTLHKKCRCLTNLFEEMRMRYERRDPRQEDLQQIEELKSVIESQDRDLRLLTEQFREMQLHERELQQRLYQQQMQQQQPPQPPRRAKNRGKQQQNQQNDQIVIEEDEQQQQQQQQQHQPQIQIQPMTASTPIVCDVIYEENEADLIQEEEQQPTPSHIVHQDQTNAKQTQIVEQQDPQSQEEVIVDVPESAIETLVDVVQSTEPVFIENIPSLDSEPNQCNEPAEESSSPVEPSEVPVIQISCLPESKPAETDVSESIPTPAIVITTENEESIEAENVEVSISTVVELPAPQQFIKVVEDPSPAKPALVEPQTEVQHKVQSMPEMIVIREPDVIAEAKAAVAATAEVSTAVDVVVCAPGTRSSST

Target ORF information:

RefSeq Version	XM_001648416.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL004099-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001648416.1

ORF Insert Sequence:

ATGAACCCGT CATATTCTAT TCTATTGAAA CGGAAACAGC ACTCAATCAC ACTCCACTTG 
TGCAACGAAC AGCGCGAGGG TGTGCTCCAG TTGTTGCATG ACAGCTTTTA TATTATACCA 
GTTGTCCCGG GTCCAGTATT GAAGGAGCTA GTGAATCAGG AATCCCGCTA CAGCGTCAAG 
CTTGAGGATG CTGAGAAGCG GGCCAGCGAG GCCCAGACTA AGGTGTACGA GCTGAAGCAA 
CGGTTGGATG AGGTGGAAAG AGAAGTTTCC TTGAAAGAAT GCAACGTAGA CCGCCTCAAA 
GCGGAACTAG ATGCAGCGTG CAAGGAATGT GAGACTATCC GTGCGCGGAT GCACTCGCAG 
AGCTCCGAAC TGGACGCACT AAGACTGAAA TTCTCCGAAC GGGAGGATGA GCTAAATCTT 
AAGTACCAAA ATTTAGAAAT AGAATACATA GAGTTAACCG AGAAGCTGAA AGATGTGCGT 
CAGTTGGCCC ACGATTTGAA CATGCAGCTC ATCAACGCCC AATCGGAAGC GGAGCGGGTC 
CAGAAGGAGA AGAATCAGCT CCTGGAGGAA CGTGCCGAGG AGCAGAAACT GGTCAAGGAA 
GCGCTCGAGT CGGCCTTGAA AGAGCGAGCG CAGATCGAGG CCAAGTGGAA GAATGATTTC 
GAGCAGCTGC GGACGGTGCA CTCCGACCGG GAGGAGCAAT TCATGGAGGA CTGCGAGTGG 
AAGATACGGT CGATGCAGAA GAACTGCAAG GAGAAGATGG AGAAGATCGA AAGCGAACAC 
AAGATGGCCA TGGACAAGAT GGCGCAGTTG GAGCAAGAGT CGAGCAAACA TATAGAGGAG 
GCCAAACACC TAAGATCTTA CGAAGCGGAG GTGGCCCAGT TACGAGGGTT GACTTACGAC 
CAAAAGGAGG CACTGAAGAC AATGACGCGC CAGATTGACC AACTCAAGGC CGACTTGCTG 
ATGGCCAATA ACAAACTGGA AGCGGAAATT GTTAAGGTGC AACAGATCAA GAACCGCTGT 
GAATACCAAC TATGTGAAAA GGAGCGTGAG GCTCTCAACC GAATAGAAAT CGCTCGAGGA 
GAGATTGCAA TGCAGTGGGA GGATCGGTTG CTTCACGAGA TGAATCGCCT AAAGATGGAA 
CTGGAGCAGA TGTACATCGA GGAGCGAGTT GCCGCCATCA ACAAGTTCAA ACGGGAAGCC 
CTGGAGGAAA CGGAAGCGCT CAAGCACAAG TTCAACATGC GCGAGAGGCA ACTCAAAGAA 
GAGATTGAAT CACTGAAAAG CACAATCCAG TTGCAAAAAC AAGCGATGGA GGCTGCCCAA 
ACGGAAGCGG ATCACAGACT AGTGCAGTCT CGAATGTTTG TAGAACGAGC AGAACGAGAG 
CATGAGACTA TTTTGGCCAA AGAAGTTTCC AAGAGGGATC AAATTATTGA TAATCTCAAA 
GAACAATACG AGAAGGAAAA GTTCGAGATG GAGCAACACT TTAGTCTGAG AATACAGCAA 
GTACAGGAAG AGTTCCAACG GGAATTGTCC GATACAACCG AATTGCTGAA AGCTACACAC 
AAGAAAGAAT TGGAACAACA ATGGAAGAAT TTGGTGAACG AGAAGGAAGA AGCACTCCAA 
CTGATGGACT CCCGACAAAG AAACCGTCTT CAGGAAGCTG AAAATGAAAT CAGAGAGCTA 
AAGATCGGCC ATCAGCGGGA AATGAAGGAC CTTCACGAGG AACAACTGTT CACGATTACC 
AGTCTGGAGG CACGCGATAC CAAAAATGCC CAGGAAATTC AAACCTTGCA CAAAAAGTGT 
CGATGTTTAA CGAATCTTTT CGAGGAAATG CGAATGCGAT ACGAACGTCG AGATCCTCGG 
CAGGAAGATT TGCAGCAGAT CGAGGAATTG AAGAGTGTGA TCGAGTCCCA GGACCGTGAT 
CTAAGACTCT TAACAGAACA ATTTCGAGAA ATGCAACTGC ACGAGAGGGA ACTGCAGCAA 
AGGTTATATC AACAGCAAAT GCAGCAGCAA CAACCTCCAC AACCTCCGAG GAGAGCGAAG 
AACCGTGGCA AACAGCAACA AAATCAACAG AATGATCAAA TAGTGATAGA AGAAGACGAA 
CAACAACAGC AGCAGCAACA ACAACAGCAT CAACCTCAGA TACAGATCCA ACCTATGACA 
GCATCTACAC CTATAGTTTG TGATGTGATC TACGAAGAGA ATGAAGCTGA TCTAATCCAA 
GAGGAAGAAC AGCAACCAAC ACCTTCTCAT ATTGTACATC AAGATCAAAC AAACGCAAAG 
CAAACACAAA TTGTCGAGCA GCAGGATCCA CAGAGCCAGG AAGAGGTGAT AGTAGACGTT 
CCAGAATCTG CCATCGAAAC TTTGGTGGAC GTTGTGCAGT CGACTGAACC TGTTTTCATC 
GAAAATATCC CAAGTTTAGA TTCAGAACCG AATCAATGTA ATGAACCTGC TGAGGAATCA 
TCCAGTCCTG TTGAACCATC AGAAGTCCCT GTCATACAAA TTAGTTGTCT CCCAGAATCA 
AAGCCTGCAG AAACTGATGT GTCTGAGTCT ATACCAACGC CTGCAATTGT TATCACTACA 
GAAAACGAAG AATCGATAGA AGCAGAAAAT GTTGAAGTAT CTATATCGAC GGTAGTAGAA 
CTTCCTGCGC CTCAACAGTT TATCAAAGTT GTAGAAGATC CATCTCCAGC AAAGCCGGCT 
CTAGTGGAAC CTCAAACCGA AGTGCAACAT AAAGTACAAA GCATGCCGGA AATGATTGTC 
ATAAGAGAAC CGGATGTGAT TGCAGAGGCC AAGGCTGCTG TTGCTGCTAC TGCTGAGGTT 
TCGACAGCTG TTGATGTGGT TGTATGTGCG CCCGGTACGC GATCTTCATC GACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5564138 ( XM_021855370.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711062.1 Aedes aegypti trichohyalin (LOC5564138), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OAe129904
Clone ID Related Accession (Same CDS sequence)	XM_021855370.1
Accession Version	XM_021855370.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	trichohyalin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTTT TCAACCTCCA AATTCCGAGT TTCTTGGCAC ATCTGCCCGA AGATACTGAC 
GATACGAGCG ACACCGAGGA CGACAGCGGT ATCGCAGATG AAACCGAAAC TGAAACCGAA 
TCGGACCCAT CGTCGCTGCC AATGGCGGTA GACTCCCTCG GGCCGCAGAT TGCACAGTTT 
CCGGATCTAA AAATTACCGA CCCGATCAGC GTGCAGAATC ATGCGTTGAA CAGCTTTTTT 
CACTACTGGA AGGAGCTAGT GAATCAGGAA TCCCGCTACA GCGTCAAGCT TGAGGATGCT 
GAGAAGCGGG CCAGCGAGGC CCAGACTAAG GTGTACGAGC TGAAGCAACG GTTGGATGAG 
GTGGAAAGAG AAGTTTCCTT GAAAGAATGC AACGTAGACC GCCTCAAAGC GGAACTAGAT 
GCAGCGTGCA AGGAATGTGA GACTATCCGT GCGCGGATGC ACTCGCAGAG CTCCGAACTG 
GACGCACTAA GACTGAAATT CTCCGAACGG GAGGATGAGC TAAATCTTAA GTACCAAAAT 
TTAGAAATAG AATACATAGA GTTAACCGAG AAGCTGAAAG ATGTGCGTCA GTTGGCCCAC 
GATTTGAACA TGCAGCTCAT CAACGCCCAA TCGGAAGCGG AGCGGGTCCA GAAGGAGAAG 
AATCAGCTCC TGGAGGAACG TGCCGAGGAG CAGAAACTGG TCAAGGAAGC GCTCGAGTCG 
GCCTTGAAAG AGCGAGCGCA GATCGAGGCC AAGTGGAAGA ATGATTTCGA GCAGCTGCGG 
ACGGTGCACT CCGACCGGGA GGAGCAATTC ATGGAGGACT GCGAGTGGAA GATACGGTCG 
ATGCAGAAGA ACTGCAAGGA GAAGATGGAG AAGATCGAAA GCGAACACAA GATGGCCATG 
GACAAGATGG CGCAGTTGGA GCAAGAGTCG AGCAAACATA TAGAGGAGGC CAAACACCTA 
AGATCTTACG AAGCGGAGGT GGCCCAGTTA CGAGGGTTGA CTTACGACCA AAAGGAGGCA 
CTGAAGACAA TGACGCGCCA GATTGACCAA CTCAAGGCCG ACTTGCTGAT GGCCAATAAC 
AAACTGGAAG CGGAAATTGT TAAGGTGCAA CAGATCAAGA ACCGCTGTGA ATACCAACTA 
TGTGAAAAGG AGCGTGAGGC TCTCAACCGA ATAGAAATCG CTCGAGGAGA GATTGCAATG 
CAGTGGGAGG ATCGGTTGCT TCACGAGATG AATCGCCTAA AGATGGAACT GGAGCAGATG 
TACATCGAGG AGCGAGTTGC CGCCATCAAC AAGTTCAAAC GGGAAGCCCT GGAGGAAACG 
GAAGCGCTCA AGCACAAGTT CAACATGCGC GAGAGGCAAC TCAAAGAAGA GATTGAATCA 
CTGAAAAGCA CAATCCAGTT GCAAAAACAA GCGATGGAGG CTGCCCAAAC GGAAGCGGAT 
CACAGACTAG TGCAGTCTCG AATGTTTGTA GAACGAGCAG AACGAGAGCA TGAGACTATT 
TTGGCCAAAG AAGTTTCCAA GAGGGATCAA ATTATTGATA ATCTCAAAGA ACAATACGAG 
AAGGAAAAGT TCGAGATGGA GCAACACTTT AGTCTGAGAA TACAGCAAGT ACAGGAAGAG 
TTCCAACGGG AATTGTCCGA TACAACCGAA TTGCTGAAAG CTACACACAA GAAAGAATTG 
GAACAACAAT GGAAGAATTT GGTGAACGAG AAGGAAGAAG CACTCCAACT GATGGACTCC 
CGACAAAGAA ACCGTCTTCA GGAAGCTGAA AATGAAATCA GAGAGCTAAA GATCGGCCAT 
CAGCGGGAAA TGAAGGACCT TCACGAGGAA CAACTGTTCA CGATTACCAG TCTGGAGGCA 
CGCGATACCA AAAATGCCCA GGAAATTCAA ACCTTGCACA AAAAGTGTCG ATGTTTAACG 
AATCTTTTCG AGGAAATGCG AATGCGATAC GAACGTCGAG ATCCTCGGCA GGAAGATTTG 
CAGCAGATCG AGGAATTGAA GAGTGTGATC GAGTCCCAGG ACCGTGATCT AAGACTCTTA 
ACAGAACAAT TTCGAGAAAT GCAACTGCAC GAGAGGGAAC TGCAGCAAAG GTTATATCAA 
CAGCAAATGC AGCAGCAACA ACCTCCACAA CCTCCGAGGA GAGCGAAGAA CCGTGGCAAA 
CAGCAACAAA ATCAACAGAA TGATCAAATA GTGATAGAAG AAGACGAACA ACAACAGCAG 
CAGCAACAAC AACAGCATCA ACCTCAGATA CAGATCCAAC CTATGACAGC ATCTACACCT 
ATAGTTTGTG ATGTGATCTA CGAAGAGAAT GAAGCTGATC TAATCCAAGA GGAAGAACAG 
CAACCAACAC CTTCTCATAT TGTACATCAA GATCAAACAA ACGCAAAGCA AACACAAATT 
GTCGAGCAGC AGGATCCACA GAGCCAGGAA GAGGTGATAG TAGACGTTCC AGAATCTGCC 
ATCGAAACTT TGGTGGACGT TGTGCAGTCG ACTGAACCTG TTTTCATCGA AAATATCCCA 
AGTTTAGATT CAGAACCGAA TCAATGTAAT GAACCTGCTG AGGAATCATC CAGTCCTGTT 
GAACCATCAG AAGTCCCTGT CATACAAATT AGTTGTCTCC CAGAATCAAA GCCTGCAGAA 
ACTGATGTGT CTGAGTCTAT ACCAACGCCT GCAATTGTTA TCACTACAGA AAACGAAGAA 
TCGATAGAAG CAGAAAATGT TGAAGTATCT ATATCGACGG TAGTAGAACT TCCTGCGCCT 
CAACAGTTTA TCAAAGTTGT AGAAGATCCA TCTCCAGCAA AGCCGGCTCT AGTGGAACCT 
CAAACCGAAG TGCAACATAA AGTACAAAGC ATGCCGGAAA TGATTGTCAT AAGAGAACCG 
GATGTGATTG CAGAGGCCAA GGCTGCTGTT GCTGCTACTG CTGAGGTTTC GACAGCTGTT 
GATGTGGTTG TATGTGCGCC CGGTACGCGA TCTTCATCGA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711062.1
CDS	387..3371
Translation	MSFFNLQIPSFLAHLPEDTDDTSDTEDDSGIADETETETESDPSSLPMAVDSLGPQIAQFPDLKITDPISVQNHALNSFFHYWKELVNQESRYSVKLEDAEKRASEAQTKVYELKQRLDEVEREVSLKECNVDRLKAELDAACKECETIRARMHSQSSELDALRLKFSEREDELNLKYQNLEIEYIELTEKLKDVRQLAHDLNMQLINAQSEAERVQKEKNQLLEERAEEQKLVKEALESALKERAQIEAKWKNDFEQLRTVHSDREEQFMEDCEWKIRSMQKNCKEKMEKIESEHKMAMDKMAQLEQESSKHIEEAKHLRSYEAEVAQLRGLTYDQKEALKTMTRQIDQLKADLLMANNKLEAEIVKVQQIKNRCEYQLCEKEREALNRIEIARGEIAMQWEDRLLHEMNRLKMELEQMYIEERVAAINKFKREALEETEALKHKFNMRERQLKEEIESLKSTIQLQKQAMEAAQTEADHRLVQSRMFVERAEREHETILAKEVSKRDQIIDNLKEQYEKEKFEMEQHFSLRIQQVQEEFQRELSDTTELLKATHKKELEQQWKNLVNEKEEALQLMDSRQRNRLQEAENEIRELKIGHQREMKDLHEEQLFTITSLEARDTKNAQEIQTLHKKCRCLTNLFEEMRMRYERRDPRQEDLQQIEELKSVIESQDRDLRLLTEQFREMQLHERELQQRLYQQQMQQQQPPQPPRRAKNRGKQQQNQQNDQIVIEEDEQQQQQQQQQHQPQIQIQPMTASTPIVCDVIYEENEADLIQEEEQQPTPSHIVHQDQTNAKQTQIVEQQDPQSQEEVIVDVPESAIETLVDVVQSTEPVFIENIPSLDSEPNQCNEPAEESSSPVEPSEVPVIQISCLPESKPAETDVSESIPTPAIVITTENEESIEAENVEVSISTVVELPAPQQFIKVVEDPSPAKPALVEPQTEVQHKVQSMPEMIVIREPDVIAEAKAAVAATAEVSTAVDVVVCAPGTRSSST

Target ORF information:

RefSeq Version	XM_021855370.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti trichohyalin (LOC5564138), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855370.1

ORF Insert Sequence:

ATGAGTTTTT TCAACCTCCA AATTCCGAGT TTCTTGGCAC ATCTGCCCGA AGATACTGAC 
GATACGAGCG ACACCGAGGA CGACAGCGGT ATCGCAGATG AAACCGAAAC TGAAACCGAA 
TCGGACCCAT CGTCGCTGCC AATGGCGGTA GACTCCCTCG GGCCGCAGAT TGCACAGTTT 
CCGGATCTAA AAATTACCGA CCCGATCAGC GTGCAGAATC ATGCGTTGAA CAGCTTTTTT 
CACTACTGGA AGGAGCTAGT GAATCAGGAA TCCCGCTACA GCGTCAAGCT TGAGGATGCT 
GAGAAGCGGG CCAGCGAGGC CCAGACTAAG GTGTACGAGC TGAAGCAACG GTTGGATGAG 
GTGGAAAGAG AAGTTTCCTT GAAAGAATGC AACGTAGACC GCCTCAAAGC GGAACTAGAT 
GCAGCGTGCA AGGAATGTGA GACTATCCGT GCGCGGATGC ACTCGCAGAG CTCCGAACTG 
GACGCACTAA GACTGAAATT CTCCGAACGG GAGGATGAGC TAAATCTTAA GTACCAAAAT 
TTAGAAATAG AATACATAGA GTTAACCGAG AAGCTGAAAG ATGTGCGTCA GTTGGCCCAC 
GATTTGAACA TGCAGCTCAT CAACGCCCAA TCGGAAGCGG AGCGGGTCCA GAAGGAGAAG 
AATCAGCTCC TGGAGGAACG TGCCGAGGAG CAGAAACTGG TCAAGGAAGC GCTCGAGTCG 
GCCTTGAAAG AGCGAGCGCA GATCGAGGCC AAGTGGAAGA ATGATTTCGA GCAGCTGCGG 
ACGGTGCACT CCGACCGGGA GGAGCAATTC ATGGAGGACT GCGAGTGGAA GATACGGTCG 
ATGCAGAAGA ACTGCAAGGA GAAGATGGAG AAGATCGAAA GCGAACACAA GATGGCCATG 
GACAAGATGG CGCAGTTGGA GCAAGAGTCG AGCAAACATA TAGAGGAGGC CAAACACCTA 
AGATCTTACG AAGCGGAGGT GGCCCAGTTA CGAGGGTTGA CTTACGACCA AAAGGAGGCA 
CTGAAGACAA TGACGCGCCA GATTGACCAA CTCAAGGCCG ACTTGCTGAT GGCCAATAAC 
AAACTGGAAG CGGAAATTGT TAAGGTGCAA CAGATCAAGA ACCGCTGTGA ATACCAACTA 
TGTGAAAAGG AGCGTGAGGC TCTCAACCGA ATAGAAATCG CTCGAGGAGA GATTGCAATG 
CAGTGGGAGG ATCGGTTGCT TCACGAGATG AATCGCCTAA AGATGGAACT GGAGCAGATG 
TACATCGAGG AGCGAGTTGC CGCCATCAAC AAGTTCAAAC GGGAAGCCCT GGAGGAAACG 
GAAGCGCTCA AGCACAAGTT CAACATGCGC GAGAGGCAAC TCAAAGAAGA GATTGAATCA 
CTGAAAAGCA CAATCCAGTT GCAAAAACAA GCGATGGAGG CTGCCCAAAC GGAAGCGGAT 
CACAGACTAG TGCAGTCTCG AATGTTTGTA GAACGAGCAG AACGAGAGCA TGAGACTATT 
TTGGCCAAAG AAGTTTCCAA GAGGGATCAA ATTATTGATA ATCTCAAAGA ACAATACGAG 
AAGGAAAAGT TCGAGATGGA GCAACACTTT AGTCTGAGAA TACAGCAAGT ACAGGAAGAG 
TTCCAACGGG AATTGTCCGA TACAACCGAA TTGCTGAAAG CTACACACAA GAAAGAATTG 
GAACAACAAT GGAAGAATTT GGTGAACGAG AAGGAAGAAG CACTCCAACT GATGGACTCC 
CGACAAAGAA ACCGTCTTCA GGAAGCTGAA AATGAAATCA GAGAGCTAAA GATCGGCCAT 
CAGCGGGAAA TGAAGGACCT TCACGAGGAA CAACTGTTCA CGATTACCAG TCTGGAGGCA 
CGCGATACCA AAAATGCCCA GGAAATTCAA ACCTTGCACA AAAAGTGTCG ATGTTTAACG 
AATCTTTTCG AGGAAATGCG AATGCGATAC GAACGTCGAG ATCCTCGGCA GGAAGATTTG 
CAGCAGATCG AGGAATTGAA GAGTGTGATC GAGTCCCAGG ACCGTGATCT AAGACTCTTA 
ACAGAACAAT TTCGAGAAAT GCAACTGCAC GAGAGGGAAC TGCAGCAAAG GTTATATCAA 
CAGCAAATGC AGCAGCAACA ACCTCCACAA CCTCCGAGGA GAGCGAAGAA CCGTGGCAAA 
CAGCAACAAA ATCAACAGAA TGATCAAATA GTGATAGAAG AAGACGAACA ACAACAGCAG 
CAGCAACAAC AACAGCATCA ACCTCAGATA CAGATCCAAC CTATGACAGC ATCTACACCT 
ATAGTTTGTG ATGTGATCTA CGAAGAGAAT GAAGCTGATC TAATCCAAGA GGAAGAACAG 
CAACCAACAC CTTCTCATAT TGTACATCAA GATCAAACAA ACGCAAAGCA AACACAAATT 
GTCGAGCAGC AGGATCCACA GAGCCAGGAA GAGGTGATAG TAGACGTTCC AGAATCTGCC 
ATCGAAACTT TGGTGGACGT TGTGCAGTCG ACTGAACCTG TTTTCATCGA AAATATCCCA 
AGTTTAGATT CAGAACCGAA TCAATGTAAT GAACCTGCTG AGGAATCATC CAGTCCTGTT 
GAACCATCAG AAGTCCCTGT CATACAAATT AGTTGTCTCC CAGAATCAAA GCCTGCAGAA 
ACTGATGTGT CTGAGTCTAT ACCAACGCCT GCAATTGTTA TCACTACAGA AAACGAAGAA 
TCGATAGAAG CAGAAAATGT TGAAGTATCT ATATCGACGG TAGTAGAACT TCCTGCGCCT 
CAACAGTTTA TCAAAGTTGT AGAAGATCCA TCTCCAGCAA AGCCGGCTCT AGTGGAACCT 
CAAACCGAAG TGCAACATAA AGTACAAAGC ATGCCGGAAA TGATTGTCAT AAGAGAACCG 
GATGTGATTG CAGAGGCCAA GGCTGCTGTT GCTGCTACTG CTGAGGTTTC GACAGCTGTT 
GATGTGGTTG TATGTGCGCC CGGTACGCGA TCTTCATCGA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5564138 ( XM_021855369.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711061.1 Aedes aegypti trichohyalin (LOC5564138), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OAe129903
Clone ID Related Accession (Same CDS sequence)	XM_021855369.1
Accession Version	XM_021855369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	uncharacterized protein LOC5564138 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGCT TTTTCAAACG CTCAAAGAGT CAAAAATCAA AACAAAAACA CGATGCAAGT 
GCAGCGAAGG TTGCAGTGCC GAACGCGTGT TTCTCTAATA CTAACAACGC AGCAACACCG 
ATGCGACTAC TCGATCAAAA CGATGGTGTT CGAGATCGTG TTGATGCTAA GGTAAGACCC 
ACTACCGGCA ATGGCGGAAG TGGTGGAGAG GGCGAAAGTG TTAAAAATAA GCAGCATCCA 
CCGCCAACGG CTACTCGCTT CAGTCCGAAT GATATAATCC GCGAGAACGG TCGTTCGTTA 
GATTTTGAGG ACGGCACGGG CAGCGTGATC GAGTTCGCAG ACGCCACCGA ATCTGACGAC 
GGGAACGCGA TCAATGAGTG CGAGGATCGA GAGGATCCCA AATTGCTGCC ACTTCAAGTA 
CGGGGGGAGA CTAACGATTC GAAATCAGCT AGTTGTAATC AAAACGCGAA CCTGCTCGAA 
AGCATCATGG CGAAAGGTAA AAACAAACGA CGCTACCAGC AGCAGCAAAA TGGCAAGAAT 
TTGCAGCAAC AACAAGGTCA CAAACAGACG GGTCAGCATC AACAATCCAA CAAAGATAAC 
CACCTGCTGC ACAAACAAGC AATCAGCGAA AAATCACACA AAGTAAGCAC AGAGAAAGTG 
AGCGACACGA TTGACGTGTC TAAGGTGACG ACGCAGCAGG CTGAAATTGT GTCTCAAGTA 
GAGCAAATTA ATGGCGAAGT TAATCATAGT GAGCATAATT CCAACCAGGA GCAAAAAGTG 
AAAATCGTTA GCAAAGATAC TGAGACTGAG CAACGACCGT GTCTCACGGC CGGTGACGTC 
AATGGAAACA ACGTCGAAGT TCACGAGGAT AAAAATAGCA TCGTGGTGCG CTCGGATACC 
GAAAGCCCAT CGGACGGTGA TCATCCCGAT GAAGATGTGA GTGTAACAAC CGAGGACCTT 
ACTGTCACTA CATCCGTGAC ATCACCATTT CCGTTAACAC AGACAACGAA CAGCGATCTC 
ACTGAGATCG CTAGCAAAGC CGGCCTAAAT GAATTATTAA AGAGCATCAA TATCGACTCA 
GTATCTTCTC GGTGTCCTGC GGCGGAACTT CATCAATCCA CCACTATTCA CGAGATTAAA 
GATGGTTTGC GCGACACGAT TGGCGGTAGT GAAATTGTAA CTAGCAAAGA CTTAACATCA 
GTCAAGGAAA TGGGCCAAAC GCCAAGCAAA ACCAGTGAAT CCGCAGGCAC TAACAGACGA 
TCGCTCACGC CCAATCGTCA ATCGCCCTGT CCAGCATCAG AAACTCGGCA ACAACCGTTG 
CTATCAGTAC AAGTGAAACA AGATGCTTCT CGGGACGATA TTAGCGACGA TCGAGATGTC 
TTCTACGAAG CGACGGAATC CTTATCCCCC GTACCTAACT CCACCCCCGT GGATAACAGT 
GTCCCGGCCG TACCGCCGTT CTCCGCGATC AAACCAGCAG GCCAGGAAAA GTCTGCACTT 
CCCAAGGCTA TCATTTCCGC TCATCAAAGT GAGCCGCGAA TCAGCCCGAA GAAACGAGTA 
GTGTTCAGTG ATCAGCTCAT AATAGATGGG AACCCCTCCC AGGAATCCGA TTTACGCAAC 
GACAGTGTTA GCAGTGCAAG TACCGAGTCA ATCCCGAATG CGTTAAATTT AGAACCACCA 
CGTTATGAGC ATGTCGTGAA AAATAATGAT GAACAAATGC ATGCCGAATC CTTACCGCCC 
GGTTCATTTG TGTTTAACCA AAGTGCAAAC GAAGTAGTCA GTGCCGATAA TAACGTCGAG 
CAAAGTGCAT TTAATAGTGA TAAGAGTGGC ATTTCTATCA GTGTTCCAAT GATCATTGTC 
AAACCGAAAC TAATCGAATT CCGTTACGAT CGCAGTGATC CAAGTGCTCC GCAAAATGGT 
CATAGCGAAG AAGAAAAAGA AAAATCACAG CCTACTGTGA TCAATGGTGA TAAGGAGTTG 
ATTGAAAATG GCACCACCTT GATCGTGGAT GAGATCATCA GCCTTCCGGA TGTGGTGCAG 
CCAACGAACA TCGACAAGGC AACCAAACAG CCCTTCAGCA CAGTTGATAA AATCAACAGT 
GAAATGAAGG AGCTAGTGAA TCAGGAATCC CGCTACAGCG TCAAGCTTGA GGATGCTGAG 
AAGCGGGCCA GCGAGGCCCA GACTAAGGTG TACGAGCTGA AGCAACGGTT GGATGAGGTG 
GAAAGAGAAG TTTCCTTGAA AGAATGCAAC GTAGACCGCC TCAAAGCGGA ACTAGATGCA 
GCGTGCAAGG AATGTGAGAC TATCCGTGCG CGGATGCACT CGCAGAGCTC CGAACTGGAC 
GCACTAAGAC TGAAATTCTC CGAACGGGAG GATGAGCTAA ATCTTAAGTA CCAAAATTTA 
GAAATAGAAT ACATAGAGTT AACCGAGAAG CTGAAAGATG TGCGTCAGTT GGCCCACGAT 
TTGAACATGC AGCTCATCAA CGCCCAATCG GAAGCGGAGC GGGTCCAGAA GGAGAAGAAT 
CAGCTCCTGG AGGAACGTGC CGAGGAGCAG AAACTGGTCA AGGAAGCGCT CGAGTCGGCC 
TTGAAAGAGC GAGCGCAGAT CGAGGCCAAG TGGAAGAATG ATTTCGAGCA GCTGCGGACG 
GTGCACTCCG ACCGGGAGGA GCAATTCATG GAGGACTGCG AGTGGAAGAT ACGGTCGATG 
CAGAAGAACT GCAAGGAGAA GATGGAGAAG ATCGAAAGCG AACACAAGAT GGCCATGGAC 
AAGATGGCGC AGTTGGAGCA AGAGTCGAGC AAACATATAG AGGAGGCCAA ACACCTAAGA 
TCTTACGAAG CGGAGGTGGC CCAGTTACGA GGGTTGACTT ACGACCAAAA GGAGGCACTG 
AAGACAATGA CGCGCCAGAT TGACCAACTC AAGGCCGACT TGCTGATGGC CAATAACAAA 
CTGGAAGCGG AAATTGTTAA GGTGCAACAG ATCAAGAACC GCTGTGAATA CCAACTATGT 
GAAAAGGAGC GTGAGGCTCT CAACCGAATA GAAATCGCTC GAGGAGAGAT TGCAATGCAG 
TGGGAGGATC GGTTGCTTCA CGAGATGAAT CGCCTAAAGA TGGAACTGGA GCAGATGTAC 
ATCGAGGAGC GAGTTGCCGC CATCAACAAG TTCAAACGGG AAGCCCTGGA GGAAACGGAA 
GCGCTCAAGC ACAAGTTCAA CATGCGCGAG AGGCAACTCA AAGAAGAGAT TGAATCACTG 
AAAAGCACAA TCCAGTTGCA AAAACAAGCG ATGGAGGCTG CCCAAACGGA AGCGGATCAC 
AGACTAGTGC AGTCTCGAAT GTTTGTAGAA CGAGCAGAAC GAGAGCATGA GACTATTTTG 
GCCAAAGAAG TTTCCAAGAG GGATCAAATT ATTGATAATC TCAAAGAACA ATACGAGAAG 
GAAAAGTTCG AGATGGAGCA ACACTTTAGT CTGAGAATAC AGCAAGTACA GGAAGAGTTC 
CAACGGGAAT TGTCCGATAC AACCGAATTG CTGAAAGCTA CACACAAGAA AGAATTGGAA 
CAACAATGGA AGAATTTGGT GAACGAGAAG GAAGAAGCAC TCCAACTGAT GGACTCCCGA 
CAAAGAAACC GTCTTCAGGA AGCTGAAAAT GAAATCAGCA TACAAAACAA AGTGGAATCA 
CCATTTTCAT CGATGCGCGA TATTAACAAC AACTGCAACT CAACCAGAAG ATTCACAGAT 
TGCGATCATT GCACACCGTT AGGGCCACTA GCGAATCTCG CTCATCTGTT GGTGGAATTT 
GTCAAGGGAG CGTATCCGAT ATTTCCGGCA CTTGTGATCT ACTACCAGTC ACATTCCCTC 
TCGTTGGCTG TGCTCACGTT CATAATGACT CTGGGGCTTA TTGTGTACCT CCAGCTGCGA 
ATGATCAATC GGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711061.1
CDS	264..4181
Translation	MFSFFKRSKSQKSKQKHDASAAKVAVPNACFSNTNNAATPMRLLDQNDGVRDRVDAKVRPTTGNGGSGGEGESVKNKQHPPPTATRFSPNDIIRENGRSLDFEDGTGSVIEFADATESDDGNAINECEDREDPKLLPLQVRGETNDSKSASCNQNANLLESIMAKGKNKRRYQQQQNGKNLQQQQGHKQTGQHQQSNKDNHLLHKQAISEKSHKVSTEKVSDTIDVSKVTTQQAEIVSQVEQINGEVNHSEHNSNQEQKVKIVSKDTETEQRPCLTAGDVNGNNVEVHEDKNSIVVRSDTESPSDGDHPDEDVSVTTEDLTVTTSVTSPFPLTQTTNSDLTEIASKAGLNELLKSINIDSVSSRCPAAELHQSTTIHEIKDGLRDTIGGSEIVTSKDLTSVKEMGQTPSKTSESAGTNRRSLTPNRQSPCPASETRQQPLLSVQVKQDASRDDISDDRDVFYEATESLSPVPNSTPVDNSVPAVPPFSAIKPAGQEKSALPKAIISAHQSEPRISPKKRVVFSDQLIIDGNPSQESDLRNDSVSSASTESIPNALNLEPPRYEHVVKNNDEQMHAESLPPGSFVFNQSANEVVSADNNVEQSAFNSDKSGISISVPMIIVKPKLIEFRYDRSDPSAPQNGHSEEEKEKSQPTVINGDKELIENGTTLIVDEIISLPDVVQPTNIDKATKQPFSTVDKINSEMKELVNQESRYSVKLEDAEKRASEAQTKVYELKQRLDEVEREVSLKECNVDRLKAELDAACKECETIRARMHSQSSELDALRLKFSEREDELNLKYQNLEIEYIELTEKLKDVRQLAHDLNMQLINAQSEAERVQKEKNQLLEERAEEQKLVKEALESALKERAQIEAKWKNDFEQLRTVHSDREEQFMEDCEWKIRSMQKNCKEKMEKIESEHKMAMDKMAQLEQESSKHIEEAKHLRSYEAEVAQLRGLTYDQKEALKTMTRQIDQLKADLLMANNKLEAEIVKVQQIKNRCEYQLCEKEREALNRIEIARGEIAMQWEDRLLHEMNRLKMELEQMYIEERVAAINKFKREALEETEALKHKFNMRERQLKEEIESLKSTIQLQKQAMEAAQTEADHRLVQSRMFVERAEREHETILAKEVSKRDQIIDNLKEQYEKEKFEMEQHFSLRIQQVQEEFQRELSDTTELLKATHKKELEQQWKNLVNEKEEALQLMDSRQRNRLQEAENEISIQNKVESPFSSMRDINNNCNSTRRFTDCDHCTPLGPLANLAHLLVEFVKGAYPIFPALVIYYQSHSLSLAVLTFIMTLGLIVYLQLRMINRD

Target ORF information:

RefSeq Version	XM_021855369.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti trichohyalin (LOC5564138), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855369.1

ORF Insert Sequence:

ATGTTTAGCT TTTTCAAACG CTCAAAGAGT CAAAAATCAA AACAAAAACA CGATGCAAGT 
GCAGCGAAGG TTGCAGTGCC GAACGCGTGT TTCTCTAATA CTAACAACGC AGCAACACCG 
ATGCGACTAC TCGATCAAAA CGATGGTGTT CGAGATCGTG TTGATGCTAA GGTAAGACCC 
ACTACCGGCA ATGGCGGAAG TGGTGGAGAG GGCGAAAGTG TTAAAAATAA GCAGCATCCA 
CCGCCAACGG CTACTCGCTT CAGTCCGAAT GATATAATCC GCGAGAACGG TCGTTCGTTA 
GATTTTGAGG ACGGCACGGG CAGCGTGATC GAGTTCGCAG ACGCCACCGA ATCTGACGAC 
GGGAACGCGA TCAATGAGTG CGAGGATCGA GAGGATCCCA AATTGCTGCC ACTTCAAGTA 
CGGGGGGAGA CTAACGATTC GAAATCAGCT AGTTGTAATC AAAACGCGAA CCTGCTCGAA 
AGCATCATGG CGAAAGGTAA AAACAAACGA CGCTACCAGC AGCAGCAAAA TGGCAAGAAT 
TTGCAGCAAC AACAAGGTCA CAAACAGACG GGTCAGCATC AACAATCCAA CAAAGATAAC 
CACCTGCTGC ACAAACAAGC AATCAGCGAA AAATCACACA AAGTAAGCAC AGAGAAAGTG 
AGCGACACGA TTGACGTGTC TAAGGTGACG ACGCAGCAGG CTGAAATTGT GTCTCAAGTA 
GAGCAAATTA ATGGCGAAGT TAATCATAGT GAGCATAATT CCAACCAGGA GCAAAAAGTG 
AAAATCGTTA GCAAAGATAC TGAGACTGAG CAACGACCGT GTCTCACGGC CGGTGACGTC 
AATGGAAACA ACGTCGAAGT TCACGAGGAT AAAAATAGCA TCGTGGTGCG CTCGGATACC 
GAAAGCCCAT CGGACGGTGA TCATCCCGAT GAAGATGTGA GTGTAACAAC CGAGGACCTT 
ACTGTCACTA CATCCGTGAC ATCACCATTT CCGTTAACAC AGACAACGAA CAGCGATCTC 
ACTGAGATCG CTAGCAAAGC CGGCCTAAAT GAATTATTAA AGAGCATCAA TATCGACTCA 
GTATCTTCTC GGTGTCCTGC GGCGGAACTT CATCAATCCA CCACTATTCA CGAGATTAAA 
GATGGTTTGC GCGACACGAT TGGCGGTAGT GAAATTGTAA CTAGCAAAGA CTTAACATCA 
GTCAAGGAAA TGGGCCAAAC GCCAAGCAAA ACCAGTGAAT CCGCAGGCAC TAACAGACGA 
TCGCTCACGC CCAATCGTCA ATCGCCCTGT CCAGCATCAG AAACTCGGCA ACAACCGTTG 
CTATCAGTAC AAGTGAAACA AGATGCTTCT CGGGACGATA TTAGCGACGA TCGAGATGTC 
TTCTACGAAG CGACGGAATC CTTATCCCCC GTACCTAACT CCACCCCCGT GGATAACAGT 
GTCCCGGCCG TACCGCCGTT CTCCGCGATC AAACCAGCAG GCCAGGAAAA GTCTGCACTT 
CCCAAGGCTA TCATTTCCGC TCATCAAAGT GAGCCGCGAA TCAGCCCGAA GAAACGAGTA 
GTGTTCAGTG ATCAGCTCAT AATAGATGGG AACCCCTCCC AGGAATCCGA TTTACGCAAC 
GACAGTGTTA GCAGTGCAAG TACCGAGTCA ATCCCGAATG CGTTAAATTT AGAACCACCA 
CGTTATGAGC ATGTCGTGAA AAATAATGAT GAACAAATGC ATGCCGAATC CTTACCGCCC 
GGTTCATTTG TGTTTAACCA AAGTGCAAAC GAAGTAGTCA GTGCCGATAA TAACGTCGAG 
CAAAGTGCAT TTAATAGTGA TAAGAGTGGC ATTTCTATCA GTGTTCCAAT GATCATTGTC 
AAACCGAAAC TAATCGAATT CCGTTACGAT CGCAGTGATC CAAGTGCTCC GCAAAATGGT 
CATAGCGAAG AAGAAAAAGA AAAATCACAG CCTACTGTGA TCAATGGTGA TAAGGAGTTG 
ATTGAAAATG GCACCACCTT GATCGTGGAT GAGATCATCA GCCTTCCGGA TGTGGTGCAG 
CCAACGAACA TCGACAAGGC AACCAAACAG CCCTTCAGCA CAGTTGATAA AATCAACAGT 
GAAATGAAGG AGCTAGTGAA TCAGGAATCC CGCTACAGCG TCAAGCTTGA GGATGCTGAG 
AAGCGGGCCA GCGAGGCCCA GACTAAGGTG TACGAGCTGA AGCAACGGTT GGATGAGGTG 
GAAAGAGAAG TTTCCTTGAA AGAATGCAAC GTAGACCGCC TCAAAGCGGA ACTAGATGCA 
GCGTGCAAGG AATGTGAGAC TATCCGTGCG CGGATGCACT CGCAGAGCTC CGAACTGGAC 
GCACTAAGAC TGAAATTCTC CGAACGGGAG GATGAGCTAA ATCTTAAGTA CCAAAATTTA 
GAAATAGAAT ACATAGAGTT AACCGAGAAG CTGAAAGATG TGCGTCAGTT GGCCCACGAT 
TTGAACATGC AGCTCATCAA CGCCCAATCG GAAGCGGAGC GGGTCCAGAA GGAGAAGAAT 
CAGCTCCTGG AGGAACGTGC CGAGGAGCAG AAACTGGTCA AGGAAGCGCT CGAGTCGGCC 
TTGAAAGAGC GAGCGCAGAT CGAGGCCAAG TGGAAGAATG ATTTCGAGCA GCTGCGGACG 
GTGCACTCCG ACCGGGAGGA GCAATTCATG GAGGACTGCG AGTGGAAGAT ACGGTCGATG 
CAGAAGAACT GCAAGGAGAA GATGGAGAAG ATCGAAAGCG AACACAAGAT GGCCATGGAC 
AAGATGGCGC AGTTGGAGCA AGAGTCGAGC AAACATATAG AGGAGGCCAA ACACCTAAGA 
TCTTACGAAG CGGAGGTGGC CCAGTTACGA GGGTTGACTT ACGACCAAAA GGAGGCACTG 
AAGACAATGA CGCGCCAGAT TGACCAACTC AAGGCCGACT TGCTGATGGC CAATAACAAA 
CTGGAAGCGG AAATTGTTAA GGTGCAACAG ATCAAGAACC GCTGTGAATA CCAACTATGT 
GAAAAGGAGC GTGAGGCTCT CAACCGAATA GAAATCGCTC GAGGAGAGAT TGCAATGCAG 
TGGGAGGATC GGTTGCTTCA CGAGATGAAT CGCCTAAAGA TGGAACTGGA GCAGATGTAC 
ATCGAGGAGC GAGTTGCCGC CATCAACAAG TTCAAACGGG AAGCCCTGGA GGAAACGGAA 
GCGCTCAAGC ACAAGTTCAA CATGCGCGAG AGGCAACTCA AAGAAGAGAT TGAATCACTG 
AAAAGCACAA TCCAGTTGCA AAAACAAGCG ATGGAGGCTG CCCAAACGGA AGCGGATCAC 
AGACTAGTGC AGTCTCGAAT GTTTGTAGAA CGAGCAGAAC GAGAGCATGA GACTATTTTG 
GCCAAAGAAG TTTCCAAGAG GGATCAAATT ATTGATAATC TCAAAGAACA ATACGAGAAG 
GAAAAGTTCG AGATGGAGCA ACACTTTAGT CTGAGAATAC AGCAAGTACA GGAAGAGTTC 
CAACGGGAAT TGTCCGATAC AACCGAATTG CTGAAAGCTA CACACAAGAA AGAATTGGAA 
CAACAATGGA AGAATTTGGT GAACGAGAAG GAAGAAGCAC TCCAACTGAT GGACTCCCGA 
CAAAGAAACC GTCTTCAGGA AGCTGAAAAT GAAATCAGCA TACAAAACAA AGTGGAATCA 
CCATTTTCAT CGATGCGCGA TATTAACAAC AACTGCAACT CAACCAGAAG ATTCACAGAT 
TGCGATCATT GCACACCGTT AGGGCCACTA GCGAATCTCG CTCATCTGTT GGTGGAATTT 
GTCAAGGGAG CGTATCCGAT ATTTCCGGCA CTTGTGATCT ACTACCAGTC ACATTCCCTC 
TCGTTGGCTG TGCTCACGTT CATAATGACT CTGGGGCTTA TTGTGTACCT CCAGCTGCGA 
ATGATCAATC GGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5564138 ( XM_021855367.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711059.1 Aedes aegypti trichohyalin (LOC5564138), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAe129901
Clone ID Related Accession (Same CDS sequence)	XM_021855367.1
Accession Version	XM_021855367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	uncharacterized protein LOC5564138 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGCT TTTTCAAACG CTCAAAGAGT CAAAAATCAA AACAAAAACA CGATGCAAGT 
GCAGCGAAGG TTGCAGTGCC GAACGCGTGT TTCTCTAATA CTAACAACGC AGCAACACCG 
ATGCGACTAC TCGATCAAAA CGATGGTGTT CGAGATCGTG TTGATGCTAA GGTAAGACCC 
ACTACCGGCA ATGGCGGAAG TGGTGGAGAG GGCGAAAGTG TTAAAAATAA GCAGCATCCA 
CCGCCAACGG CTACTCGCTT CAGTCCGAAT GATATAATCC GCGAGAACGG TCGTTCGTTA 
GATTTTGAGG ACGGCACGGG CAGCGTGATC GAGTTCGCAG ACGCCACCGA ATCTGACGAC 
GGGAACGCGA TCAATGAGTG CGAGGATCGA GAGGATCCCA AATTGCTGCC ACTTCAAGTA 
CGGGGGGAGA CTAACGATTC GAAATCAGCT AGTTGTAATC AAAACGCGAA CCTGCTCGAA 
AGCATCATGG CGAAAGGTAA AAACAAACGA CGCTACCAGC AGCAGCAAAA TGGCAAGAAT 
TTGCAGCAAC AACAAGGTCA CAAACAGACG GGTCAGCATC AACAATCCAA CAAAGATAAC 
CACCTGCTGC ACAAACAAGC AATCAGCGAA AAATCACACA AAGTAAGCAC AGAGAAAGTG 
AGCGACACGA TTGACGTGTC TAAGGTGACG ACGCAGCAGG CTGAAATTGT GTCTCAAGTA 
GAGCAAATTA ATGGCGAAGT TAATCATAGT GAGCATAATT CCAACCAGGA GCAAAAAGTG 
AAAATCGTTA GCAAAGATAC TGAGACTGAG CAACGACCGT GTCTCACGGC CGGTGACGTC 
AATGGAAACA ACGTCGAAGT TCACGAGGAT AAAAATAGCA TCGTGGTGCG CTCGGATACC 
GAAAGCCCAT CGGACGGTGA TCATCCCGAT GAAGATGTGA GTGTAACAAC CGAGGACCTT 
ACTGTCACTA CATCCGTGAC ATCACCATTT CCGTTAACAC AGACAACGAA CAGCGATCTC 
ACTGAGATCG CTAGCAAAGC CGGCCTAAAT GAATTATTAA AGAGCATCAA TATCGACTCA 
GTATCTTCTC GGTGTCCTGC GGCGGAACTT CATCAATCCA CCACTATTCA CGAGATTAAA 
GATGGTTTGC GCGACACGAT TGGCGGTAGT GAAATTGTAA CTAGCAAAGA CTTAACATCA 
GTCAAGGAAA TGGGCCAAAC GCCAAGCAAA ACCAGTGAAT CCGCAGGCAC TAACAGACGA 
TCGCTCACGC CCAATCGTCA ATCGCCCTGT CCAGCATCAG AAACTCGGCA ACAACCGTTG 
CTATCAGTAC AAGTGAAACA AGATGCTTCT CGGGACGATA TTAGCGACGA TCGAGATGTC 
TTCTACGAAG CGACGGAATC CTTATCCCCC GTACCTAACT CCACCCCCGT GGATAACAGT 
GTCCCGGCCG TACCGCCGTT CTCCGCGATC AAACCAGCAG GCCAGGAAAA GTCTGCACTT 
CCCAAGGCTA TCATTTCCGC TCATCAAAGT GAGCCGCGAA TCAGCCCGAA GAAACGAGTA 
GTGTTCAGTG ATCAGCTCAT AATAGATGGG AACCCCTCCC AGGAATCCGA TTTACGCAAC 
GACAGTGTTA GCAGTGCAAG TACCGAGTCA ATCCCGAATG CGTTAAATTT AGAACCACCA 
CGTTATGAGC ATGTCGTGAA AAATAATGAT GAACAAATGC ATGCCGAATC CTTACCGCCC 
GGTTCATTTG TGTTTAACCA AAGTGCAAAC GAAGTAGTCA GTGCCGATAA TAACGTCGAG 
CAAAGTGCAT TTAATAGTGA TAAGAGTGGC ATTTCTATCA GTGTTCCAAT GATCATTGTC 
AAACCGAAAC TAATCGAATT CCGTTACGAT CGCAGTGATC CAAGTGCTCC GCAAAATGGT 
CATAGCGAAG AAGAAAAAGA AAAATCACAG CCTACTGTGA TCAATGGTGA TAAGGAGTTG 
ATTGAAAATG GCACCACCTT GATCGTGGAT GAGATCATCA GCCTTCCGGA TGTGGTGCAG 
CCAACGAACA TCGACAAGGC AACCAAACAG CCCTTCAGCA CAGTTGATAA AATCAACAGT 
GAAATGAAGG AGCTAGTGAA TCAGGAATCC CGCTACAGCG TCAAGCTTGA GGATGCTGAG 
AAGCGGGCCA GCGAGGCCCA GACTAAGGTG TACGAGCTGA AGCAACGGTT GGATGAGGTG 
GAAAGAGAAG TTTCCTTGAA AGAATGCAAC GTAGACCGCC TCAAAGCGGA ACTAGATGCA 
GCGTGCAAGG AATGTGAGAC TATCCGTGCG CGGATGCACT CGCAGAGCTC CGAACTGGAC 
GCACTAAGAC TGAAATTCTC CGAACGGGAG GATGAGCTAA ATCTTAAGTA CCAAAATTTA 
GAAATAGAAT ACATAGAGTT AACCGAGAAG CTGAAAGATG TGCGTCAGTT GGCCCACGAT 
TTGAACATGC AGCTCATCAA CGCCCAATCG GAAGCGGAGC GGGTCCAGAA GGAGAAGAAT 
CAGCTCCTGG AGGAACGTGC CGAGGAGCAG AAACTGGTCA AGGAAGCGCT CGAGTCGGCC 
TTGAAAGAGC GAGCGCAGAT CGAGGCCAAG TGGAAGAATG ATTTCGAGCA GCTGCGGACG 
GTGCACTCCG ACCGGGAGGA GCAATTCATG GAGGACTGCG AGTGGAAGAT ACGGTCGATG 
CAGAAGAACT GCAAGGAGAA GATGGAGAAG ATCGAAAGCG AACACAAGAT GGCCATGGAC 
AAGATGGCGC AGTTGGAGCA AGAGTCGAGC AAACATATAG AGGAGGCCAA ACACCTAAGA 
TCTTACGAAG CGGAGGTGGC CCAGTTACGA GGGTTGACTT ACGACCAAAA GGAGGCACTG 
AAGACAATGA CGCGCCAGAT TGACCAACTC AAGGCCGACT TGCTGATGGC CAATAACAAA 
CTGGAAGCGG AAATTGTTAA GGTGCAACAG ATCAAGAACC GCTGTGAATA CCAACTATGT 
GAAAAGGAGC GTGAGGCTCT CAACCGAATA GAAATCGCTC GAGGAGAGAT TGCAATGCAG 
TGGGAGGATC GGTTGCTTCA CGAGATGAAT CGCCTAAAGA TGGAACTGGA GCAGATGTAC 
ATCGAGGAGC GAGTTGCCGC CATCAACAAG TTCAAACGGG AAGCCCTGGA GGAAACGGAA 
GCGCTCAAGC ACAAGTTCAA CATGCGCGAG AGGCAACTCA AAGAAGAGAT TGAATCACTG 
AAAAGCACAA TCCAGTTGCA AAAACAAGCG ATGGAGGCTG CCCAAACGGA AGCGGATCAC 
AGACTAGTGC AGTCTCGAAT GTTTGTAGAA CGAGCAGAAC GAGAGCATGA GACTATTTTG 
GCCAAAGAAG TTTCCAAGAG GGATCAAATT ATTGATAATC TCAAAGAACA ATACGAGAAG 
GAAAAGTTCG AGATGGAGCA ACACTTTAGT CTGAGAATAC AGCAAGTACA GGAAGAGTTC 
CAACGGGAAT TGTCCGATAC AACCGAATTG CTGAAAGCTA CACACAAGAA AGAATTGGAA 
CAACAATGGA AGAATTTGGT GAACGAGAAG GAAGAAGCAC TCCAACTGAT GGACTCCCGA 
CAAAGAAACC GTCTTCAGGA AGCTGAAAAT GAAATCAGAG AGCTAAAGAT CGGCCATCAG 
CGGGAAATGA AGGACCTTCA CGAGGAACAA CTGTTCACGA TTACCAGTCT GGAGGCACGC 
GATACCAAAA ATGCCCAGGA AATTCAAACC TTGCACAAAA AGTGTCGATG TTTAACGAAT 
CTTTTCGAGG AAATGCGAAT GCGATACGAA CGTCGAGATC CTCGGCAGGA AGATTTGCAG 
CAGATCGAGG AATTGAAGAG TGTGATCGAG TCCCAGGACC GTGATCTAAG ACTCTTAACA 
GAACAATTTC GAGAAATGCA ACTGCACGAG AGGGAACTGC AGCAAAGGTT ATATCAACAG 
CAAATGCAGC AGCAACAACC TCCACAACCT CCGAGGAGAG CGAAGAACCG TGGCAAACAG 
CAACAAAATC AACAGAATGA TCAAATAGTG ATAGAAGAAG ACGAACAACA ACAGCAGCAG 
CAACAACAAC AGCATCAACC TCAGATACAG ATCCAACCTA TGACAGCATC TACACCTATA 
GTTTGTGATG TGATCTACGA AGAGAATGAA GCTGATCTAA TCCAAGAGGA AGAACAGCAA 
CCAACACCTT CTCATATTGT ACATCAAGAT CAAACAAACG CAAAGCAAAC ACAAATTGTC 
GAGCAGCAGG ATCCACAGAG CCAGGAAGAG GTGATAGTAG ACGTTCCAGA ATCTGCCATC 
GAAACTTTGG TGGACGTTGT GCAGTCGACT GAACCTGTTT TCATCGAAAA TATCCCAAGT 
TTAGATTCAG AACCGAATCA ATGTAATGAA CCTGCTGAGG AATCATCCAG TCCTGTTGAA 
CCATCAGAAG TCCCTGTCAT ACAAATTAGT TGTCTCCCAG AATCAAAGCC TGCAGAAACT 
GATGTGTCTG AGTCTATACC AACGCCTGCA ATTGTTATCA CTACAGAAAA CGAAGAATCG 
ATAGAAGCAG AAAATGTTGA AGTATCTATA TCGACGGTAG TAGAACTTCC TGCGCCTCAA 
CAGTTTATCA AAGTTGTAGA AGATCCATCT CCAGCAAAGC CGGCTCTAGT GGAACCTCAA 
ACCGAAGTGC AACATAAAGT ACAAAGCATG CCGGAAATGA TTGTCATAAG AGAACCGGAT 
GTGATTGCAG AGGCCAAGGC TGCTGTTGCT GCTACTGCTG AGGTTTCGAC AGCTGTTGAT 
GTGGTTGTAT GTGCGCCCGG TACGCGATCT TCATCGACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711059.1
CDS	337..5178
Translation	MFSFFKRSKSQKSKQKHDASAAKVAVPNACFSNTNNAATPMRLLDQNDGVRDRVDAKVRPTTGNGGSGGEGESVKNKQHPPPTATRFSPNDIIRENGRSLDFEDGTGSVIEFADATESDDGNAINECEDREDPKLLPLQVRGETNDSKSASCNQNANLLESIMAKGKNKRRYQQQQNGKNLQQQQGHKQTGQHQQSNKDNHLLHKQAISEKSHKVSTEKVSDTIDVSKVTTQQAEIVSQVEQINGEVNHSEHNSNQEQKVKIVSKDTETEQRPCLTAGDVNGNNVEVHEDKNSIVVRSDTESPSDGDHPDEDVSVTTEDLTVTTSVTSPFPLTQTTNSDLTEIASKAGLNELLKSINIDSVSSRCPAAELHQSTTIHEIKDGLRDTIGGSEIVTSKDLTSVKEMGQTPSKTSESAGTNRRSLTPNRQSPCPASETRQQPLLSVQVKQDASRDDISDDRDVFYEATESLSPVPNSTPVDNSVPAVPPFSAIKPAGQEKSALPKAIISAHQSEPRISPKKRVVFSDQLIIDGNPSQESDLRNDSVSSASTESIPNALNLEPPRYEHVVKNNDEQMHAESLPPGSFVFNQSANEVVSADNNVEQSAFNSDKSGISISVPMIIVKPKLIEFRYDRSDPSAPQNGHSEEEKEKSQPTVINGDKELIENGTTLIVDEIISLPDVVQPTNIDKATKQPFSTVDKINSEMKELVNQESRYSVKLEDAEKRASEAQTKVYELKQRLDEVEREVSLKECNVDRLKAELDAACKECETIRARMHSQSSELDALRLKFSEREDELNLKYQNLEIEYIELTEKLKDVRQLAHDLNMQLINAQSEAERVQKEKNQLLEERAEEQKLVKEALESALKERAQIEAKWKNDFEQLRTVHSDREEQFMEDCEWKIRSMQKNCKEKMEKIESEHKMAMDKMAQLEQESSKHIEEAKHLRSYEAEVAQLRGLTYDQKEALKTMTRQIDQLKADLLMANNKLEAEIVKVQQIKNRCEYQLCEKEREALNRIEIARGEIAMQWEDRLLHEMNRLKMELEQMYIEERVAAINKFKREALEETEALKHKFNMRERQLKEEIESLKSTIQLQKQAMEAAQTEADHRLVQSRMFVERAEREHETILAKEVSKRDQIIDNLKEQYEKEKFEMEQHFSLRIQQVQEEFQRELSDTTELLKATHKKELEQQWKNLVNEKEEALQLMDSRQRNRLQEAENEIRELKIGHQREMKDLHEEQLFTITSLEARDTKNAQEIQTLHKKCRCLTNLFEEMRMRYERRDPRQEDLQQIEELKSVIESQDRDLRLLTEQFREMQLHERELQQRLYQQQMQQQQPPQPPRRAKNRGKQQQNQQNDQIVIEEDEQQQQQQQQQHQPQIQIQPMTASTPIVCDVIYEENEADLIQEEEQQPTPSHIVHQDQTNAKQTQIVEQQDPQSQEEVIVDVPESAIETLVDVVQSTEPVFIENIPSLDSEPNQCNEPAEESSSPVEPSEVPVIQISCLPESKPAETDVSESIPTPAIVITTENEESIEAENVEVSISTVVELPAPQQFIKVVEDPSPAKPALVEPQTEVQHKVQSMPEMIVIREPDVIAEAKAAVAATAEVSTAVDVVVCAPGTRSSST

Target ORF information:

RefSeq Version	XM_021855367.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti trichohyalin (LOC5564138), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855367.1

ORF Insert Sequence:

ATGTTTAGCT TTTTCAAACG CTCAAAGAGT CAAAAATCAA AACAAAAACA CGATGCAAGT 
GCAGCGAAGG TTGCAGTGCC GAACGCGTGT TTCTCTAATA CTAACAACGC AGCAACACCG 
ATGCGACTAC TCGATCAAAA CGATGGTGTT CGAGATCGTG TTGATGCTAA GGTAAGACCC 
ACTACCGGCA ATGGCGGAAG TGGTGGAGAG GGCGAAAGTG TTAAAAATAA GCAGCATCCA 
CCGCCAACGG CTACTCGCTT CAGTCCGAAT GATATAATCC GCGAGAACGG TCGTTCGTTA 
GATTTTGAGG ACGGCACGGG CAGCGTGATC GAGTTCGCAG ACGCCACCGA ATCTGACGAC 
GGGAACGCGA TCAATGAGTG CGAGGATCGA GAGGATCCCA AATTGCTGCC ACTTCAAGTA 
CGGGGGGAGA CTAACGATTC GAAATCAGCT AGTTGTAATC AAAACGCGAA CCTGCTCGAA 
AGCATCATGG CGAAAGGTAA AAACAAACGA CGCTACCAGC AGCAGCAAAA TGGCAAGAAT 
TTGCAGCAAC AACAAGGTCA CAAACAGACG GGTCAGCATC AACAATCCAA CAAAGATAAC 
CACCTGCTGC ACAAACAAGC AATCAGCGAA AAATCACACA AAGTAAGCAC AGAGAAAGTG 
AGCGACACGA TTGACGTGTC TAAGGTGACG ACGCAGCAGG CTGAAATTGT GTCTCAAGTA 
GAGCAAATTA ATGGCGAAGT TAATCATAGT GAGCATAATT CCAACCAGGA GCAAAAAGTG 
AAAATCGTTA GCAAAGATAC TGAGACTGAG CAACGACCGT GTCTCACGGC CGGTGACGTC 
AATGGAAACA ACGTCGAAGT TCACGAGGAT AAAAATAGCA TCGTGGTGCG CTCGGATACC 
GAAAGCCCAT CGGACGGTGA TCATCCCGAT GAAGATGTGA GTGTAACAAC CGAGGACCTT 
ACTGTCACTA CATCCGTGAC ATCACCATTT CCGTTAACAC AGACAACGAA CAGCGATCTC 
ACTGAGATCG CTAGCAAAGC CGGCCTAAAT GAATTATTAA AGAGCATCAA TATCGACTCA 
GTATCTTCTC GGTGTCCTGC GGCGGAACTT CATCAATCCA CCACTATTCA CGAGATTAAA 
GATGGTTTGC GCGACACGAT TGGCGGTAGT GAAATTGTAA CTAGCAAAGA CTTAACATCA 
GTCAAGGAAA TGGGCCAAAC GCCAAGCAAA ACCAGTGAAT CCGCAGGCAC TAACAGACGA 
TCGCTCACGC CCAATCGTCA ATCGCCCTGT CCAGCATCAG AAACTCGGCA ACAACCGTTG 
CTATCAGTAC AAGTGAAACA AGATGCTTCT CGGGACGATA TTAGCGACGA TCGAGATGTC 
TTCTACGAAG CGACGGAATC CTTATCCCCC GTACCTAACT CCACCCCCGT GGATAACAGT 
GTCCCGGCCG TACCGCCGTT CTCCGCGATC AAACCAGCAG GCCAGGAAAA GTCTGCACTT 
CCCAAGGCTA TCATTTCCGC TCATCAAAGT GAGCCGCGAA TCAGCCCGAA GAAACGAGTA 
GTGTTCAGTG ATCAGCTCAT AATAGATGGG AACCCCTCCC AGGAATCCGA TTTACGCAAC 
GACAGTGTTA GCAGTGCAAG TACCGAGTCA ATCCCGAATG CGTTAAATTT AGAACCACCA 
CGTTATGAGC ATGTCGTGAA AAATAATGAT GAACAAATGC ATGCCGAATC CTTACCGCCC 
GGTTCATTTG TGTTTAACCA AAGTGCAAAC GAAGTAGTCA GTGCCGATAA TAACGTCGAG 
CAAAGTGCAT TTAATAGTGA TAAGAGTGGC ATTTCTATCA GTGTTCCAAT GATCATTGTC 
AAACCGAAAC TAATCGAATT CCGTTACGAT CGCAGTGATC CAAGTGCTCC GCAAAATGGT 
CATAGCGAAG AAGAAAAAGA AAAATCACAG CCTACTGTGA TCAATGGTGA TAAGGAGTTG 
ATTGAAAATG GCACCACCTT GATCGTGGAT GAGATCATCA GCCTTCCGGA TGTGGTGCAG 
CCAACGAACA TCGACAAGGC AACCAAACAG CCCTTCAGCA CAGTTGATAA AATCAACAGT 
GAAATGAAGG AGCTAGTGAA TCAGGAATCC CGCTACAGCG TCAAGCTTGA GGATGCTGAG 
AAGCGGGCCA GCGAGGCCCA GACTAAGGTG TACGAGCTGA AGCAACGGTT GGATGAGGTG 
GAAAGAGAAG TTTCCTTGAA AGAATGCAAC GTAGACCGCC TCAAAGCGGA ACTAGATGCA 
GCGTGCAAGG AATGTGAGAC TATCCGTGCG CGGATGCACT CGCAGAGCTC CGAACTGGAC 
GCACTAAGAC TGAAATTCTC CGAACGGGAG GATGAGCTAA ATCTTAAGTA CCAAAATTTA 
GAAATAGAAT ACATAGAGTT AACCGAGAAG CTGAAAGATG TGCGTCAGTT GGCCCACGAT 
TTGAACATGC AGCTCATCAA CGCCCAATCG GAAGCGGAGC GGGTCCAGAA GGAGAAGAAT 
CAGCTCCTGG AGGAACGTGC CGAGGAGCAG AAACTGGTCA AGGAAGCGCT CGAGTCGGCC 
TTGAAAGAGC GAGCGCAGAT CGAGGCCAAG TGGAAGAATG ATTTCGAGCA GCTGCGGACG 
GTGCACTCCG ACCGGGAGGA GCAATTCATG GAGGACTGCG AGTGGAAGAT ACGGTCGATG 
CAGAAGAACT GCAAGGAGAA GATGGAGAAG ATCGAAAGCG AACACAAGAT GGCCATGGAC 
AAGATGGCGC AGTTGGAGCA AGAGTCGAGC AAACATATAG AGGAGGCCAA ACACCTAAGA 
TCTTACGAAG CGGAGGTGGC CCAGTTACGA GGGTTGACTT ACGACCAAAA GGAGGCACTG 
AAGACAATGA CGCGCCAGAT TGACCAACTC AAGGCCGACT TGCTGATGGC CAATAACAAA 
CTGGAAGCGG AAATTGTTAA GGTGCAACAG ATCAAGAACC GCTGTGAATA CCAACTATGT 
GAAAAGGAGC GTGAGGCTCT CAACCGAATA GAAATCGCTC GAGGAGAGAT TGCAATGCAG 
TGGGAGGATC GGTTGCTTCA CGAGATGAAT CGCCTAAAGA TGGAACTGGA GCAGATGTAC 
ATCGAGGAGC GAGTTGCCGC CATCAACAAG TTCAAACGGG AAGCCCTGGA GGAAACGGAA 
GCGCTCAAGC ACAAGTTCAA CATGCGCGAG AGGCAACTCA AAGAAGAGAT TGAATCACTG 
AAAAGCACAA TCCAGTTGCA AAAACAAGCG ATGGAGGCTG CCCAAACGGA AGCGGATCAC 
AGACTAGTGC AGTCTCGAAT GTTTGTAGAA CGAGCAGAAC GAGAGCATGA GACTATTTTG 
GCCAAAGAAG TTTCCAAGAG GGATCAAATT ATTGATAATC TCAAAGAACA ATACGAGAAG 
GAAAAGTTCG AGATGGAGCA ACACTTTAGT CTGAGAATAC AGCAAGTACA GGAAGAGTTC 
CAACGGGAAT TGTCCGATAC AACCGAATTG CTGAAAGCTA CACACAAGAA AGAATTGGAA 
CAACAATGGA AGAATTTGGT GAACGAGAAG GAAGAAGCAC TCCAACTGAT GGACTCCCGA 
CAAAGAAACC GTCTTCAGGA AGCTGAAAAT GAAATCAGAG AGCTAAAGAT CGGCCATCAG 
CGGGAAATGA AGGACCTTCA CGAGGAACAA CTGTTCACGA TTACCAGTCT GGAGGCACGC 
GATACCAAAA ATGCCCAGGA AATTCAAACC TTGCACAAAA AGTGTCGATG TTTAACGAAT 
CTTTTCGAGG AAATGCGAAT GCGATACGAA CGTCGAGATC CTCGGCAGGA AGATTTGCAG 
CAGATCGAGG AATTGAAGAG TGTGATCGAG TCCCAGGACC GTGATCTAAG ACTCTTAACA 
GAACAATTTC GAGAAATGCA ACTGCACGAG AGGGAACTGC AGCAAAGGTT ATATCAACAG 
CAAATGCAGC AGCAACAACC TCCACAACCT CCGAGGAGAG CGAAGAACCG TGGCAAACAG 
CAACAAAATC AACAGAATGA TCAAATAGTG ATAGAAGAAG ACGAACAACA ACAGCAGCAG 
CAACAACAAC AGCATCAACC TCAGATACAG ATCCAACCTA TGACAGCATC TACACCTATA 
GTTTGTGATG TGATCTACGA AGAGAATGAA GCTGATCTAA TCCAAGAGGA AGAACAGCAA 
CCAACACCTT CTCATATTGT ACATCAAGAT CAAACAAACG CAAAGCAAAC ACAAATTGTC 
GAGCAGCAGG ATCCACAGAG CCAGGAAGAG GTGATAGTAG ACGTTCCAGA ATCTGCCATC 
GAAACTTTGG TGGACGTTGT GCAGTCGACT GAACCTGTTT TCATCGAAAA TATCCCAAGT 
TTAGATTCAG AACCGAATCA ATGTAATGAA CCTGCTGAGG AATCATCCAG TCCTGTTGAA 
CCATCAGAAG TCCCTGTCAT ACAAATTAGT TGTCTCCCAG AATCAAAGCC TGCAGAAACT 
GATGTGTCTG AGTCTATACC AACGCCTGCA ATTGTTATCA CTACAGAAAA CGAAGAATCG 
ATAGAAGCAG AAAATGTTGA AGTATCTATA TCGACGGTAG TAGAACTTCC TGCGCCTCAA 
CAGTTTATCA AAGTTGTAGA AGATCCATCT CCAGCAAAGC CGGCTCTAGT GGAACCTCAA 
ACCGAAGTGC AACATAAAGT ACAAAGCATG CCGGAAATGA TTGTCATAAG AGAACCGGAT 
GTGATTGCAG AGGCCAAGGC TGCTGTTGCT GCTACTGCTG AGGTTTCGAC AGCTGTTGAT 
GTGGTTGTAT GTGCGCCCGG TACGCGATCT TCATCGACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5564138 ( XM_021855368.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021711060.1 Aedes aegypti trichohyalin (LOC5564138), transcript variant X3, mRNA.

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CloneID	OAe129902
Clone ID Related Accession (Same CDS sequence)	XM_021855368.1
Accession Version	XM_021855368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	interaptin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGCT TTTTCAAACG CTCAAAGAGT CAAAAATCAA AACAAAAACA CGATGCAAGT 
GCAGCGAAGG TTGCAGTGCC GAACGCGTGT TTCTCTAATA CTAACAACGC AGCAACACCG 
ATGCGACTAC TCGATCAAAA CGATGGTGTT CGAGATCGTG TTGATGCTAA GGTAAGACCC 
ACTACCGGCA ATGGCGGAAG TGGTGGAGAG GGCGAAAGTG TTAAAAATAA GCAGCATCCA 
CCGCCAACGG CTACTCGCTT CAGTCCGAAT GATATAATCC GCGAGAACGG TCGTTCGTTA 
GATTTTGAGG ACGGCACGGG CAGCGTGATC GAGTTCGCAG ACGCCACCGA ATCTGACGAC 
GGGAACGCGA TCAATGAGTG CGAGGATCGA GAGGATCCCA AATTGCTGCC ACTTCAAGTA 
CGGGGGGAGA CTAACGATTC GAAATCAGCT AGTTGTAATC AAAACGCGAA CCTGCTCGAA 
AGCATCATGG CGAAAGGTAA AAACAAACGA CGCTACCAGC AGCAGCAAAA TGGCAAGAAT 
TTGCAGCAAC AACAAGGTCA CAAACAGACG GGTCAGCATC AACAATCCAA CAAAGATAAC 
CACCTGCTGC ACAAACAAGC AATCAGCGAA AAATCACACA AAGTAAGCAC AGAGAAAGTG 
AGCGACACGA TTGACGTGTC TAAGGTGACG ACGCAGCAGG CTGAAATTGT GTCTCAAGTA 
GAGCAAATTA ATGGCGAAGT TAATCATAGT GAGCATAATT CCAACCAGGA GCAAAAAGTG 
AAAATCGTTA GCAAAGATAC TGAGACTGAG CAACGACCGT GTCTCACGGC CGGTGACGTC 
AATGGAAACA ACGTCGAAGT TCACGAGGAT AAAAATAGCA TCGTGGTGCG CTCGGATACC 
GAAAGCCCAT CGGACGGTGA TCATCCCGAT GAAGATGTGA GTGTAACAAC CGAGGACCTT 
ACTGTCACTA CATCCGTGAC ATCACCATTT CCGTTAACAC AGACAACGAA CAGCGATCTC 
ACTGAGATCG CTAGCAAAGC CGGCCTAAAT GAATTATTAA AGAGCATCAA TATCGACTCA 
GTATCTTCTC GGTGTCCTGC GGCGGAACTT CATCAATCCA CCACTATTCA CGAGATTAAA 
GATGGTTTGC GCGACACGAT TGGCGGTAGT GAAATTGTAA CTAGCAAAGA CTTAACATCA 
GTCAAGGAAA TGGGCCAAAC GCCAAGCAAA ACCAGTGAAT CCGCAGGCAC TAACAGACGA 
TCGCTCACGC CCAATCGTCA ATCGCCCTGT CCAGCATCAG AAACTCGGCA ACAACCGTTG 
CTATCAGTAC AAGTGAAACA AGATGCTTCT CGGGACGATA TTAGCGACGA TCGAGATGTC 
TTCTACGAAG CGACGGAATC CTTATCCCCC GTACCTAACT CCACCCCCGT GGATAACAGT 
GTCCCGGCCG TACCGCCGTT CTCCGCGATC AAACCAGCAG GCCAGGAAAA GTCTGCACTT 
CCCAAGGCTA TCATTTCCGC TCATCAAAGT GAGCCGCGAA TCAGCCCGAA GAAACGAGTA 
GTGTTCAGTG ATCAGCTCAT AATAGATGGG AACCCCTCCC AGGAATCCGA TTTACGCAAC 
GACAGTGTTA GCAGTGCAAG TACCGAGTCA ATCCCGAATG CGTTAAATTT AGAACCACCA 
CGTTATGAGC ATGTCGTGAA AAATAATGAT GAACAAATGC ATGCCGAATC CTTACCGCCC 
GGTTCATTTG TGTTTAACCA AAGTGCAAAC GAAGTAGTCA GTGCCGATAA TAACGTCGAG 
CAAAGTGCAT TTAATAGTGA TAAGAGTGGC ATTTCTATCA GTGTTCCAAT GATCATTGTC 
AAACCGAAAC TAATCGAATT CCGTTACGAT CGCAGTGATC CAAGTGCTCC GCAAAATGGT 
CATAGCGAAG AAGAAAAAGA AAAATCACAG CCTACTGTGA TCAATGGTGA TAAGGAGTTG 
ATTGAAAATG GCACCACCTT GATCGTGGAT GAGATCATCA GCCTTCCGGA TGTGGTGCAG 
CCAACGAACA TCGACAAGGC AACCAAACAG CCCTTCAGCA CAGTTGATAA AATCAACAGT 
GAAATGAAGG AGCTAGTGAA TCAGGAATCC CGCTACAGCG TCAAGCTTGA GGATGCTGAG 
AAGCGGGCCA GCGAGGCCCA GACTAAGGTG TACGAGCTGA AGCAACGGTT GGATGAGGTG 
GAAAGAGAAG TTTCCTTGAA AGAATGCAAC GTAGACCGCC TCAAAGCGGA ACTAGATGCA 
GCGTGCAAGG AATGTGAGAC TATCCGTGCG CGGATGCACT CGCAGAGCTC CGAACTGGAC 
GCACTAAGAC TGAAATTCTC CGAACGGGAG GATGAGCTAA ATCTTAAGTA CCAAAATTTA 
GAAATAGAAT ACATAGAGTT AACCGAGAAG CTGAAAGATG TGCGTCAGTT GGCCCACGAT 
TTGAACATGC AGCTCATCAA CGCCCAATCG GAAGCGGAGC GGGTCCAGAA GGAGAAGAAT 
CAGCTCCTGG AGGAACGTGC CGAGGAGCAG AAACTGGTCA AGGAAGCGCT CGAGTCGGCC 
TTGAAAGAGC GAGCGCAGAT CGAGGCCAAG TGGAAGAATG ATTTCGAGCA GCTGCGGACG 
GTGCACTCCG ACCGGGAGGA GCAATTCATG GAGGACTGCG AGTGGAAGAT ACGGTCGATG 
CAGAAGAACT GCAAGGAGAA GATGGAGAAG ATCGAAAGCG AACACAAGAT GGCCATGGAC 
AAGATGGCGC AGTTGGAGCA AGAGTCGAGC AAACATATAG AGGAGGCCAA ACACCTAAGA 
TCTTACGAAG CGGAGGTGGC CCAGTTACGA GGGTTGACTT ACGACCAAAA GGAGGCACTG 
AAGACAATGA CGCGCCAGAT TGACCAACTC AAGGCCGACT TGCTGATGGC CAATAACAAA 
CTGGAAGCGG AAATTGTTAA GGTGCAACAG ATCAAGAACC GCTGTGAATA CCAACTATGT 
GAAAAGGAGC GTGAGGCTCT CAACCGAATA GAAATCGCTC GAGGAGAGAT TGCAATGCAG 
TGGGAGGATC GGTTGCTTCA CGAGATGAAT CGCCTAAAGA TGGAACTGGA GCAGATGTAC 
ATCGAGGAGC GAGTTGCCGC CATCAACAAG TTCAAACGGG AAGCCCTGGA GGAAACGGAA 
GCGCTCAAGC ACAAGTTCAA CATGCGCGAG AGGCAACTCA AAGAAGAGAT TGAATCACTG 
AAAAGCACAA TCCAGTTGCA AAAACAAGCG ATGGAGGCTG CCCAAACGGA AGCGGATCAC 
AGACTAGTGC AGTCTCGAAT GTTTGTAGAA CGAGCAGAAC GAGAGCATGA GACTATTTTG 
GCCAAAGAAG TTTCCAAGAG GGATCAAATT ATTGATAATC TCAAAGAACA ATACGAGAAG 
GAAAAGTTCG AGATGGAGCA ACACTTTAGT CTGAGAATAC AGCAAGTACA GGAAGAGTTC 
CAACGGGAAT TGTCCGATAC AACCGAATTG CTGAAAGCTA CACACAAGAA AGAATTGGAA 
CAACAATGGA AGAATTTGGT GAACGAGAAG GAAGAAGCAC TCCAACTGAT GGACTCCCGA 
CAAAGAAACC GTCTTCAGGA AGCTGAAAAT GAAATCAGTT TCGAGGAAAT GCGAATGCGA 
TACGAACGTC GAGATCCTCG GCAGGAAGAT TTGCAGCAGA TCGAGGAATT GAAGAGTGTG 
ATCGAGTCCC AGGACCGTGA TCTAAGACTC TTAACAGAAC AATTTCGAGA AATGCAACTG 
CACGAGAGGG AACTGCAGCA AAGGTTATAT CAACAGCAAA TGCAGCAGCA ACAACCTCCA 
CAACCTCCGA GGAGAGCGAA GAACCGTGGC AAACAGCAAC AAAATCAACA GAATGATCAA 
ATAGTGATAG AAGAAGACGA ACAACAACAG CAGCAGCAAC AACAACAGCA TCAACCTCAG 
ATACAGATCC AACCTATGAC AGCATCTACA CCTATAGTTT GTGATGTGAT CTACGAAGAG 
AATGAAGCTG ATCTAATCCA AGAGGAAGAA CAGCAACCAA CACCTTCTCA TATTGTACAT 
CAAGATCAAA CAAACGCAAA GCAAACACAA ATTGTCGAGC AGCAGGATCC ACAGAGCCAG 
GAAGAGGTGA TAGTAGACGT TCCAGAATCT GCCATCGAAA CTTTGGTGGA CGTTGTGCAG 
TCGACTGAAC CTGTTTTCAT CGAAAATATC CCAAGTTTAG ATTCAGAACC GAATCAATGT 
AATGAACCTG CTGAGGAATC ATCCAGTCCT GTTGAACCAT CAGAAGTCCC TGTCATACAA 
ATTAGTTGTC TCCCAGAATC AAAGCCTGCA GAAACTGATG TGTCTGAGTC TATACCAACG 
CCTGCAATTG TTATCACTAC AGAAAACGAA GAATCGATAG AAGCAGAAAA TGTTGAAGTA 
TCTATATCGA CGGTAGTAGA ACTTCCTGCG CCTCAACAGT TTATCAAAGT TGTAGAAGAT 
CCATCTCCAG CAAAGCCGGC TCTAGTGGAA CCTCAAACCG AAGTGCAACA TAAAGTACAA 
AGCATGCCGG AAATGATTGT CATAAGAGAA CCGGATGTGA TTGCAGAGGC CAAGGCTGCT 
GTTGCTGCTA CTGCTGAGGT TTCGACAGCT GTTGATGTGG TTGTATGTGC GCCCGGTACG 
CGATCTTCAT CGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021711060.1
CDS	245..4942
Translation	MFSFFKRSKSQKSKQKHDASAAKVAVPNACFSNTNNAATPMRLLDQNDGVRDRVDAKVRPTTGNGGSGGEGESVKNKQHPPPTATRFSPNDIIRENGRSLDFEDGTGSVIEFADATESDDGNAINECEDREDPKLLPLQVRGETNDSKSASCNQNANLLESIMAKGKNKRRYQQQQNGKNLQQQQGHKQTGQHQQSNKDNHLLHKQAISEKSHKVSTEKVSDTIDVSKVTTQQAEIVSQVEQINGEVNHSEHNSNQEQKVKIVSKDTETEQRPCLTAGDVNGNNVEVHEDKNSIVVRSDTESPSDGDHPDEDVSVTTEDLTVTTSVTSPFPLTQTTNSDLTEIASKAGLNELLKSINIDSVSSRCPAAELHQSTTIHEIKDGLRDTIGGSEIVTSKDLTSVKEMGQTPSKTSESAGTNRRSLTPNRQSPCPASETRQQPLLSVQVKQDASRDDISDDRDVFYEATESLSPVPNSTPVDNSVPAVPPFSAIKPAGQEKSALPKAIISAHQSEPRISPKKRVVFSDQLIIDGNPSQESDLRNDSVSSASTESIPNALNLEPPRYEHVVKNNDEQMHAESLPPGSFVFNQSANEVVSADNNVEQSAFNSDKSGISISVPMIIVKPKLIEFRYDRSDPSAPQNGHSEEEKEKSQPTVINGDKELIENGTTLIVDEIISLPDVVQPTNIDKATKQPFSTVDKINSEMKELVNQESRYSVKLEDAEKRASEAQTKVYELKQRLDEVEREVSLKECNVDRLKAELDAACKECETIRARMHSQSSELDALRLKFSEREDELNLKYQNLEIEYIELTEKLKDVRQLAHDLNMQLINAQSEAERVQKEKNQLLEERAEEQKLVKEALESALKERAQIEAKWKNDFEQLRTVHSDREEQFMEDCEWKIRSMQKNCKEKMEKIESEHKMAMDKMAQLEQESSKHIEEAKHLRSYEAEVAQLRGLTYDQKEALKTMTRQIDQLKADLLMANNKLEAEIVKVQQIKNRCEYQLCEKEREALNRIEIARGEIAMQWEDRLLHEMNRLKMELEQMYIEERVAAINKFKREALEETEALKHKFNMRERQLKEEIESLKSTIQLQKQAMEAAQTEADHRLVQSRMFVERAEREHETILAKEVSKRDQIIDNLKEQYEKEKFEMEQHFSLRIQQVQEEFQRELSDTTELLKATHKKELEQQWKNLVNEKEEALQLMDSRQRNRLQEAENEISFEEMRMRYERRDPRQEDLQQIEELKSVIESQDRDLRLLTEQFREMQLHERELQQRLYQQQMQQQQPPQPPRRAKNRGKQQQNQQNDQIVIEEDEQQQQQQQQQHQPQIQIQPMTASTPIVCDVIYEENEADLIQEEEQQPTPSHIVHQDQTNAKQTQIVEQQDPQSQEEVIVDVPESAIETLVDVVQSTEPVFIENIPSLDSEPNQCNEPAEESSSPVEPSEVPVIQISCLPESKPAETDVSESIPTPAIVITTENEESIEAENVEVSISTVVELPAPQQFIKVVEDPSPAKPALVEPQTEVQHKVQSMPEMIVIREPDVIAEAKAAVAATAEVSTAVDVVVCAPGTRSSST

Target ORF information:

RefSeq Version	XM_021855368.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti trichohyalin (LOC5564138), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021855368.1

ORF Insert Sequence:

ATGTTTAGCT TTTTCAAACG CTCAAAGAGT CAAAAATCAA AACAAAAACA CGATGCAAGT 
GCAGCGAAGG TTGCAGTGCC GAACGCGTGT TTCTCTAATA CTAACAACGC AGCAACACCG 
ATGCGACTAC TCGATCAAAA CGATGGTGTT CGAGATCGTG TTGATGCTAA GGTAAGACCC 
ACTACCGGCA ATGGCGGAAG TGGTGGAGAG GGCGAAAGTG TTAAAAATAA GCAGCATCCA 
CCGCCAACGG CTACTCGCTT CAGTCCGAAT GATATAATCC GCGAGAACGG TCGTTCGTTA 
GATTTTGAGG ACGGCACGGG CAGCGTGATC GAGTTCGCAG ACGCCACCGA ATCTGACGAC 
GGGAACGCGA TCAATGAGTG CGAGGATCGA GAGGATCCCA AATTGCTGCC ACTTCAAGTA 
CGGGGGGAGA CTAACGATTC GAAATCAGCT AGTTGTAATC AAAACGCGAA CCTGCTCGAA 
AGCATCATGG CGAAAGGTAA AAACAAACGA CGCTACCAGC AGCAGCAAAA TGGCAAGAAT 
TTGCAGCAAC AACAAGGTCA CAAACAGACG GGTCAGCATC AACAATCCAA CAAAGATAAC 
CACCTGCTGC ACAAACAAGC AATCAGCGAA AAATCACACA AAGTAAGCAC AGAGAAAGTG 
AGCGACACGA TTGACGTGTC TAAGGTGACG ACGCAGCAGG CTGAAATTGT GTCTCAAGTA 
GAGCAAATTA ATGGCGAAGT TAATCATAGT GAGCATAATT CCAACCAGGA GCAAAAAGTG 
AAAATCGTTA GCAAAGATAC TGAGACTGAG CAACGACCGT GTCTCACGGC CGGTGACGTC 
AATGGAAACA ACGTCGAAGT TCACGAGGAT AAAAATAGCA TCGTGGTGCG CTCGGATACC 
GAAAGCCCAT CGGACGGTGA TCATCCCGAT GAAGATGTGA GTGTAACAAC CGAGGACCTT 
ACTGTCACTA CATCCGTGAC ATCACCATTT CCGTTAACAC AGACAACGAA CAGCGATCTC 
ACTGAGATCG CTAGCAAAGC CGGCCTAAAT GAATTATTAA AGAGCATCAA TATCGACTCA 
GTATCTTCTC GGTGTCCTGC GGCGGAACTT CATCAATCCA CCACTATTCA CGAGATTAAA 
GATGGTTTGC GCGACACGAT TGGCGGTAGT GAAATTGTAA CTAGCAAAGA CTTAACATCA 
GTCAAGGAAA TGGGCCAAAC GCCAAGCAAA ACCAGTGAAT CCGCAGGCAC TAACAGACGA 
TCGCTCACGC CCAATCGTCA ATCGCCCTGT CCAGCATCAG AAACTCGGCA ACAACCGTTG 
CTATCAGTAC AAGTGAAACA AGATGCTTCT CGGGACGATA TTAGCGACGA TCGAGATGTC 
TTCTACGAAG CGACGGAATC CTTATCCCCC GTACCTAACT CCACCCCCGT GGATAACAGT 
GTCCCGGCCG TACCGCCGTT CTCCGCGATC AAACCAGCAG GCCAGGAAAA GTCTGCACTT 
CCCAAGGCTA TCATTTCCGC TCATCAAAGT GAGCCGCGAA TCAGCCCGAA GAAACGAGTA 
GTGTTCAGTG ATCAGCTCAT AATAGATGGG AACCCCTCCC AGGAATCCGA TTTACGCAAC 
GACAGTGTTA GCAGTGCAAG TACCGAGTCA ATCCCGAATG CGTTAAATTT AGAACCACCA 
CGTTATGAGC ATGTCGTGAA AAATAATGAT GAACAAATGC ATGCCGAATC CTTACCGCCC 
GGTTCATTTG TGTTTAACCA AAGTGCAAAC GAAGTAGTCA GTGCCGATAA TAACGTCGAG 
CAAAGTGCAT TTAATAGTGA TAAGAGTGGC ATTTCTATCA GTGTTCCAAT GATCATTGTC 
AAACCGAAAC TAATCGAATT CCGTTACGAT CGCAGTGATC CAAGTGCTCC GCAAAATGGT 
CATAGCGAAG AAGAAAAAGA AAAATCACAG CCTACTGTGA TCAATGGTGA TAAGGAGTTG 
ATTGAAAATG GCACCACCTT GATCGTGGAT GAGATCATCA GCCTTCCGGA TGTGGTGCAG 
CCAACGAACA TCGACAAGGC AACCAAACAG CCCTTCAGCA CAGTTGATAA AATCAACAGT 
GAAATGAAGG AGCTAGTGAA TCAGGAATCC CGCTACAGCG TCAAGCTTGA GGATGCTGAG 
AAGCGGGCCA GCGAGGCCCA GACTAAGGTG TACGAGCTGA AGCAACGGTT GGATGAGGTG 
GAAAGAGAAG TTTCCTTGAA AGAATGCAAC GTAGACCGCC TCAAAGCGGA ACTAGATGCA 
GCGTGCAAGG AATGTGAGAC TATCCGTGCG CGGATGCACT CGCAGAGCTC CGAACTGGAC 
GCACTAAGAC TGAAATTCTC CGAACGGGAG GATGAGCTAA ATCTTAAGTA CCAAAATTTA 
GAAATAGAAT ACATAGAGTT AACCGAGAAG CTGAAAGATG TGCGTCAGTT GGCCCACGAT 
TTGAACATGC AGCTCATCAA CGCCCAATCG GAAGCGGAGC GGGTCCAGAA GGAGAAGAAT 
CAGCTCCTGG AGGAACGTGC CGAGGAGCAG AAACTGGTCA AGGAAGCGCT CGAGTCGGCC 
TTGAAAGAGC GAGCGCAGAT CGAGGCCAAG TGGAAGAATG ATTTCGAGCA GCTGCGGACG 
GTGCACTCCG ACCGGGAGGA GCAATTCATG GAGGACTGCG AGTGGAAGAT ACGGTCGATG 
CAGAAGAACT GCAAGGAGAA GATGGAGAAG ATCGAAAGCG AACACAAGAT GGCCATGGAC 
AAGATGGCGC AGTTGGAGCA AGAGTCGAGC AAACATATAG AGGAGGCCAA ACACCTAAGA 
TCTTACGAAG CGGAGGTGGC CCAGTTACGA GGGTTGACTT ACGACCAAAA GGAGGCACTG 
AAGACAATGA CGCGCCAGAT TGACCAACTC AAGGCCGACT TGCTGATGGC CAATAACAAA 
CTGGAAGCGG AAATTGTTAA GGTGCAACAG ATCAAGAACC GCTGTGAATA CCAACTATGT 
GAAAAGGAGC GTGAGGCTCT CAACCGAATA GAAATCGCTC GAGGAGAGAT TGCAATGCAG 
TGGGAGGATC GGTTGCTTCA CGAGATGAAT CGCCTAAAGA TGGAACTGGA GCAGATGTAC 
ATCGAGGAGC GAGTTGCCGC CATCAACAAG TTCAAACGGG AAGCCCTGGA GGAAACGGAA 
GCGCTCAAGC ACAAGTTCAA CATGCGCGAG AGGCAACTCA AAGAAGAGAT TGAATCACTG 
AAAAGCACAA TCCAGTTGCA AAAACAAGCG ATGGAGGCTG CCCAAACGGA AGCGGATCAC 
AGACTAGTGC AGTCTCGAAT GTTTGTAGAA CGAGCAGAAC GAGAGCATGA GACTATTTTG 
GCCAAAGAAG TTTCCAAGAG GGATCAAATT ATTGATAATC TCAAAGAACA ATACGAGAAG 
GAAAAGTTCG AGATGGAGCA ACACTTTAGT CTGAGAATAC AGCAAGTACA GGAAGAGTTC 
CAACGGGAAT TGTCCGATAC AACCGAATTG CTGAAAGCTA CACACAAGAA AGAATTGGAA 
CAACAATGGA AGAATTTGGT GAACGAGAAG GAAGAAGCAC TCCAACTGAT GGACTCCCGA 
CAAAGAAACC GTCTTCAGGA AGCTGAAAAT GAAATCAGTT TCGAGGAAAT GCGAATGCGA 
TACGAACGTC GAGATCCTCG GCAGGAAGAT TTGCAGCAGA TCGAGGAATT GAAGAGTGTG 
ATCGAGTCCC AGGACCGTGA TCTAAGACTC TTAACAGAAC AATTTCGAGA AATGCAACTG 
CACGAGAGGG AACTGCAGCA AAGGTTATAT CAACAGCAAA TGCAGCAGCA ACAACCTCCA 
CAACCTCCGA GGAGAGCGAA GAACCGTGGC AAACAGCAAC AAAATCAACA GAATGATCAA 
ATAGTGATAG AAGAAGACGA ACAACAACAG CAGCAGCAAC AACAACAGCA TCAACCTCAG 
ATACAGATCC AACCTATGAC AGCATCTACA CCTATAGTTT GTGATGTGAT CTACGAAGAG 
AATGAAGCTG ATCTAATCCA AGAGGAAGAA CAGCAACCAA CACCTTCTCA TATTGTACAT 
CAAGATCAAA CAAACGCAAA GCAAACACAA ATTGTCGAGC AGCAGGATCC ACAGAGCCAG 
GAAGAGGTGA TAGTAGACGT TCCAGAATCT GCCATCGAAA CTTTGGTGGA CGTTGTGCAG 
TCGACTGAAC CTGTTTTCAT CGAAAATATC CCAAGTTTAG ATTCAGAACC GAATCAATGT 
AATGAACCTG CTGAGGAATC ATCCAGTCCT GTTGAACCAT CAGAAGTCCC TGTCATACAA 
ATTAGTTGTC TCCCAGAATC AAAGCCTGCA GAAACTGATG TGTCTGAGTC TATACCAACG 
CCTGCAATTG TTATCACTAC AGAAAACGAA GAATCGATAG AAGCAGAAAA TGTTGAAGTA 
TCTATATCGA CGGTAGTAGA ACTTCCTGCG CCTCAACAGT TTATCAAAGT TGTAGAAGAT 
CCATCTCCAG CAAAGCCGGC TCTAGTGGAA CCTCAAACCG AAGTGCAACA TAAAGTACAA 
AGCATGCCGG AAATGATTGT CATAAGAGAA CCGGATGTGA TTGCAGAGGC CAAGGCTGCT 
GTTGCTGCTA CTGCTGAGGT TTCGACAGCT GTTGATGTGG TTGTATGTGC GCCCGGTACG 
CGATCTTCAT CGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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LOC5564138 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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