LINJ_29_2770 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_29_2770
Entrez Gene ID	5070834
Full Name	putative helicase
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001466756.1	XP_001466793.1	putative helicase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_29_2770 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_29_2770 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc04358	XM_001466756.1 Latest version!	Leishmania infantum JPCM5 putative helicase partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_29_2770 ( XM_001466756.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001466793.1 Leishmania infantum JPCM5 putative helicase partial mRNA.

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CloneID	OLc04358
Clone ID Related Accession (Same CDS sequence)	XM_001466756.1
Accession Version	XM_001466756.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009413). COMPLETENESS: incomplete on both ends.

ATGGTGGCCG AGGTGGACGT GAGCGGTATT ACCGTGTCTT TCCCCTTCGA ACCGTATCCA 
GCACAGGTGG AGTTCATGCG CAGCGTCGTG AAGTGCCTCC AGCACGGCTT CAACGGGTTG 
TTGGAGTCCC CCACCGGTAC TGGCAAGACG CTCTGCTTGC TGTGTAGCAC GCTTGGCTGG 
CTTGCGGCAA CCTCGCAAGG TGCAGTCCTT CGGCACACTA GCGACCAAGA TCAGAAAGGT 
CGCGGTAAGT ACAACCACAA GGTGGTGTAC TGCAGCCGAA CGCACGCTCA GCTGACGCAG 
GTGGTGCGTG AGCTGAAGCG CACGTCGTAT GCACAGTGCT TCACCATGGC CGTCCTGGGC 
TCTCGTGAGC ACATGTGCTT GAACAAAGAG GTGACGCGGC TCCCGTCGTC GCAGGCGCAG 
CACGCCATGT GCAGTGCGCT TCGGTCGGAG CGCAACTGCC GCTTCTTCCG CGGTTTGCTG 
TCTGCGGCCG CTGGCGCCAG CCTTTTGTCC CCTGAATGCG CGGTGCACGA CATGGAGGAC 
TTGATGCGAG AGGGAAGCCG AAGCGGGTTC TGCCCATACT TCCACGAGCG TGACGCGGCG 
AAGGACGCGG ACGTTGTACT CATGCCGTAC AACTACGTCC TTGACCCGTC CCTGCACAAG 
CAGCTGCCCT TTGAGCTGGC GGACTGTATT CTCATTGTGG ACGAGGCGCA CAACCTGCCC 
TCCGTGCTCA GCAGCTCGGG TTGTCAGACG TTGTCGCCGC TAGACGTGAC CACCGCCATC 
CACGACTGCT CACGGGCCAT CGCAATGCAT CGCCTCACCA CAAAGGGGGC AGAGCTGGAC 
GAGGACGTGG CGGCGGAAGA CGAGCAGGAG CTGGCGTCGC TGAAGATTCT GCTGAGCAGG 
CTAGAGATGT GCGTGTACGC GGAGCCTATG ACAGAGGCGA CGGCGGCGCC CACGCCGACG 
CAGTGCGATG TGGTGCGGGA TGGTTCGTAC ATGTTCGTTT TCTTGGAGAA GGCCTTTATC 
ACTCGCGAGG TCTTTGGGGC GCAGTCGGAC GGCGTCACCT CGGCGTCGGG GCTAGCAGGG 
ACGATGGGGA AGTGCGTGAC GCTTCTGGCG GATAGCGAGC GACCCGCAAC GAGCATGGCG 
CGAGTGCAGG AGTTTCTCAC GCGTGTCTTC GCCTTTGATA TGGCCCATCT CGACTCCACC 
AGGTTTGTGC TGCAGCAGCA TCTCGTCGCT GCCAAGGCCG CCCGCACACT CGGCTTCTGG 
GAGCTGGATA ACACGCGCTT GATGCGGCAG GTCGTGTTGC CGCTGCATTC GGTTCTGCTC 
ACGAGCGGCA CGCTGTCTCC GCTTGACCAG TTCGCGGCGG AGCTGGGCAT GGAGTTTCAG 
GTGCGCCTGA AGGGAAATCA CGTGATCCAG CCAGATCAAG TGCTCGGCGG GGTCCTCTGC 
CGCGGGCCGA GCGGCGAGAA GCTGAATGGC GGCTTTTCGT TCCGCAGCAG CGTGGACTAC 
CGCGTAGGTC TCGGCATGAG TCTCGCCAAC ATCGCTCGCA ACACACCTGG CGGCACTCTT 
GTCTTCTTCC CCAGTTATGC CTCTATGAAC TCTGTGGTCG AGCTGTGGCA GGCGGGGAGC 
GGGCGTGCAG GGGACACCAA AACAGTGTGG GGCATGCTGT CGGAGCTAAA GCCGATCTTC 
GTGGAGCCGA GCAACTCCAA CGACTTGCCA ACCATCGTAC AGAGCTTCCA AAAAGAAGTG 
GACACGTCAC CCCTCCGCGG CGCGATTCTG CTGGCGGTGT GCCGTGGCAA GATCAGCGAG 
GGCATCGACT TTGCCGACAA CCACGGCCGC TGCGTGCTCG TCGCGGGCAT CCCGTACGCC 
AACCACACCG ACCTCTTCGT TCGCCTTAAG CGCGACTACA TCACATCCGT GGCGCCGCAG 
CGGCCCCTGG TGCATGGAAA ACTATTCACG GGCGACGACT GGTACCGGAA TGAGGCGATG 
CGGGCCGTCA ACCAGTGCGT CGGCCGCGTG ATTCGGCACA AGGACGACTA CGGCGTTGTC 
CTGCTCGCTG ATGAGCGCTT CGAGGGACTG CTGGGTAGCG TGTCGGAGTG GGTGCAGCGG 
CGCACGCGTG TCTTTTCCGA CTTCCGCGGC GCCTACGCTG CCGTTGCGCA GTTTTTTGGC 
GGCCGGCGCC ATCGCGCGAC TGAGACGGCT GCCATTCCAT ATGTGTTGAT CGAGAACGGT 
GCAAACGCCG CAGCCGAGCT GCCGTCCACT GCGACGCTCG CGAGGCTGTA CGCAGATGCG 
CAGGCGCGTC AGCGCGAAGA GCATGTGCAA GAGACACGCC GACGTCGCTT CGAGGAAGTG 
CGCGAGGCCA AGGCCAGCGC GTGCAACAAT GCCGTCGCGT CCGCGACGTC GCCGTTTGCA 
CCTGCGTCGT TTGCCGGGTG TTCAACGACG GGCCGCGCTG GTGGGACCCC TGCGATCCCG 
CAGCGAACCT CTGCGGAGAC ACCGGAGGCG ACAGTGGTGT CATCGGAGCG AGAGACGGCG 
GGCATGCGCG ATACCGGGCA GATGGGGCAG CCGGACGGGC CTCCAAAGCT CGGGCCTACG 
TCAAAGGAGT TCTGCGAGTT TCTCAAGGCA CGCGTGCCAG TAGAGGCGTA TCATCGCTTC 
AAGATGGTGT TGGCCCAGCT GGCGACCCTC CGCCCGCTTC TGCGAACATC GCCGACGGCA 
GCCGCGGATG GACTCGCAAG GCTTCTCGTG CCGTTGCGGT CCGTCTTTGG CGCCACAGAC 
GTTGAGTACC ATCACAGTCT CTTTGCTGAG TTCGGCCGCC ACATACCTGA GGAATTTCGG 
CCACTCTACG CGGACCTCCT CAAATCCAAC GGGCTGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001466793.1
CDS	1..2862
Translation	MVAEVDVSGITVSFPFEPYPAQVEFMRSVVKCLQHGFNGLLESPTGTGKTLCLLCSTLGWLAATSQGAVLRHTSDQDQKGRGKYNHKVVYCSRTHAQLTQVVRELKRTSYAQCFTMAVLGSREHMCLNKEVTRLPSSQAQHAMCSALRSERNCRFFRGLLSAAAGASLLSPECAVHDMEDLMREGSRSGFCPYFHERDAAKDADVVLMPYNYVLDPSLHKQLPFELADCILIVDEAHNLPSVLSSSGCQTLSPLDVTTAIHDCSRAIAMHRLTTKGAELDEDVAAEDEQELASLKILLSRLEMCVYAEPMTEATAAPTPTQCDVVRDGSYMFVFLEKAFITREVFGAQSDGVTSASGLAGTMGKCVTLLADSERPATSMARVQEFLTRVFAFDMAHLDSTRFVLQQHLVAAKAARTLGFWELDNTRLMRQVVLPLHSVLLTSGTLSPLDQFAAELGMEFQVRLKGNHVIQPDQVLGGVLCRGPSGEKLNGGFSFRSSVDYRVGLGMSLANIARNTPGGTLVFFPSYASMNSVVELWQAGSGRAGDTKTVWGMLSELKPIFVEPSNSNDLPTIVQSFQKEVDTSPLRGAILLAVCRGKISEGIDFADNHGRCVLVAGIPYANHTDLFVRLKRDYITSVAPQRPLVHGKLFTGDDWYRNEAMRAVNQCVGRVIRHKDDYGVVLLADERFEGLLGSVSEWVQRRTRVFSDFRGAYAAVAQFFGGRRHRATETAAIPYVLIENGANAAAELPSTATLARLYADAQARQREEHVQETRRRRFEEVREAKASACNNAVASATSPFAPASFAGCSTTGRAGGTPAIPQRTSAETPEATVVSSERETAGMRDTGQMGQPDGPPKLGPTSKEFCEFLKARVPVEAYHRFKMVLAQLATLRPLLRTSPTAAADGLARLLVPLRSVFGATDVEYHHSLFAEFGRHIPEEFRPLYADLLKSNGLM

Target ORF information:

RefSeq Version	XM_001466756.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative helicase partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001466756.1

ORF Insert Sequence:

ATGGTGGCCG AGGTGGACGT GAGCGGTATT ACCGTGTCTT TCCCCTTCGA ACCGTATCCA 
GCACAGGTGG AGTTCATGCG CAGCGTCGTG AAGTGCCTCC AGCACGGCTT CAACGGGTTG 
TTGGAGTCCC CCACCGGTAC TGGCAAGACG CTCTGCTTGC TGTGTAGCAC GCTTGGCTGG 
CTTGCGGCAA CCTCGCAAGG TGCAGTCCTT CGGCACACTA GCGACCAAGA TCAGAAAGGT 
CGCGGTAAGT ACAACCACAA GGTGGTGTAC TGCAGCCGAA CGCACGCTCA GCTGACGCAG 
GTGGTGCGTG AGCTGAAGCG CACGTCGTAT GCACAGTGCT TCACCATGGC CGTCCTGGGC 
TCTCGTGAGC ACATGTGCTT GAACAAAGAG GTGACGCGGC TCCCGTCGTC GCAGGCGCAG 
CACGCCATGT GCAGTGCGCT TCGGTCGGAG CGCAACTGCC GCTTCTTCCG CGGTTTGCTG 
TCTGCGGCCG CTGGCGCCAG CCTTTTGTCC CCTGAATGCG CGGTGCACGA CATGGAGGAC 
TTGATGCGAG AGGGAAGCCG AAGCGGGTTC TGCCCATACT TCCACGAGCG TGACGCGGCG 
AAGGACGCGG ACGTTGTACT CATGCCGTAC AACTACGTCC TTGACCCGTC CCTGCACAAG 
CAGCTGCCCT TTGAGCTGGC GGACTGTATT CTCATTGTGG ACGAGGCGCA CAACCTGCCC 
TCCGTGCTCA GCAGCTCGGG TTGTCAGACG TTGTCGCCGC TAGACGTGAC CACCGCCATC 
CACGACTGCT CACGGGCCAT CGCAATGCAT CGCCTCACCA CAAAGGGGGC AGAGCTGGAC 
GAGGACGTGG CGGCGGAAGA CGAGCAGGAG CTGGCGTCGC TGAAGATTCT GCTGAGCAGG 
CTAGAGATGT GCGTGTACGC GGAGCCTATG ACAGAGGCGA CGGCGGCGCC CACGCCGACG 
CAGTGCGATG TGGTGCGGGA TGGTTCGTAC ATGTTCGTTT TCTTGGAGAA GGCCTTTATC 
ACTCGCGAGG TCTTTGGGGC GCAGTCGGAC GGCGTCACCT CGGCGTCGGG GCTAGCAGGG 
ACGATGGGGA AGTGCGTGAC GCTTCTGGCG GATAGCGAGC GACCCGCAAC GAGCATGGCG 
CGAGTGCAGG AGTTTCTCAC GCGTGTCTTC GCCTTTGATA TGGCCCATCT CGACTCCACC 
AGGTTTGTGC TGCAGCAGCA TCTCGTCGCT GCCAAGGCCG CCCGCACACT CGGCTTCTGG 
GAGCTGGATA ACACGCGCTT GATGCGGCAG GTCGTGTTGC CGCTGCATTC GGTTCTGCTC 
ACGAGCGGCA CGCTGTCTCC GCTTGACCAG TTCGCGGCGG AGCTGGGCAT GGAGTTTCAG 
GTGCGCCTGA AGGGAAATCA CGTGATCCAG CCAGATCAAG TGCTCGGCGG GGTCCTCTGC 
CGCGGGCCGA GCGGCGAGAA GCTGAATGGC GGCTTTTCGT TCCGCAGCAG CGTGGACTAC 
CGCGTAGGTC TCGGCATGAG TCTCGCCAAC ATCGCTCGCA ACACACCTGG CGGCACTCTT 
GTCTTCTTCC CCAGTTATGC CTCTATGAAC TCTGTGGTCG AGCTGTGGCA GGCGGGGAGC 
GGGCGTGCAG GGGACACCAA AACAGTGTGG GGCATGCTGT CGGAGCTAAA GCCGATCTTC 
GTGGAGCCGA GCAACTCCAA CGACTTGCCA ACCATCGTAC AGAGCTTCCA AAAAGAAGTG 
GACACGTCAC CCCTCCGCGG CGCGATTCTG CTGGCGGTGT GCCGTGGCAA GATCAGCGAG 
GGCATCGACT TTGCCGACAA CCACGGCCGC TGCGTGCTCG TCGCGGGCAT CCCGTACGCC 
AACCACACCG ACCTCTTCGT TCGCCTTAAG CGCGACTACA TCACATCCGT GGCGCCGCAG 
CGGCCCCTGG TGCATGGAAA ACTATTCACG GGCGACGACT GGTACCGGAA TGAGGCGATG 
CGGGCCGTCA ACCAGTGCGT CGGCCGCGTG ATTCGGCACA AGGACGACTA CGGCGTTGTC 
CTGCTCGCTG ATGAGCGCTT CGAGGGACTG CTGGGTAGCG TGTCGGAGTG GGTGCAGCGG 
CGCACGCGTG TCTTTTCCGA CTTCCGCGGC GCCTACGCTG CCGTTGCGCA GTTTTTTGGC 
GGCCGGCGCC ATCGCGCGAC TGAGACGGCT GCCATTCCAT ATGTGTTGAT CGAGAACGGT 
GCAAACGCCG CAGCCGAGCT GCCGTCCACT GCGACGCTCG CGAGGCTGTA CGCAGATGCG 
CAGGCGCGTC AGCGCGAAGA GCATGTGCAA GAGACACGCC GACGTCGCTT CGAGGAAGTG 
CGCGAGGCCA AGGCCAGCGC GTGCAACAAT GCCGTCGCGT CCGCGACGTC GCCGTTTGCA 
CCTGCGTCGT TTGCCGGGTG TTCAACGACG GGCCGCGCTG GTGGGACCCC TGCGATCCCG 
CAGCGAACCT CTGCGGAGAC ACCGGAGGCG ACAGTGGTGT CATCGGAGCG AGAGACGGCG 
GGCATGCGCG ATACCGGGCA GATGGGGCAG CCGGACGGGC CTCCAAAGCT CGGGCCTACG 
TCAAAGGAGT TCTGCGAGTT TCTCAAGGCA CGCGTGCCAG TAGAGGCGTA TCATCGCTTC 
AAGATGGTGT TGGCCCAGCT GGCGACCCTC CGCCCGCTTC TGCGAACATC GCCGACGGCA 
GCCGCGGATG GACTCGCAAG GCTTCTCGTG CCGTTGCGGT CCGTCTTTGG CGCCACAGAC 
GTTGAGTACC ATCACAGTCT CTTTGCTGAG TTCGGCCGCC ACATACCTGA GGAATTTCGG 
CCACTCTACG CGGACCTCCT CAAATCCAAC GGGCTGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_29_2770 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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