LINJ_02_0680 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_02_0680
Entrez Gene ID	5066203
Full Name	ATP-dependent Clp protease subunit, heat shock protein 78 (HSP78)
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001462721.1	XP_001462758.1	ATP-dependent Clp protease subunit, heat shock protein 78 (HSP78)

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_02_0680 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_02_0680 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc00227	XM_001462721.1 Latest version!	Leishmania infantum JPCM5 ATP-dependent Clp protease subunit, heat shock protein 78 (HSP78) partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_02_0680 ( XM_001462721.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001462758.1 Leishmania infantum JPCM5 ATP-dependent Clp protease subunit, heat shock protein 78 (HSP78) partial mRNA.

Price & Availability↑

CloneID	OLc00227
Clone ID Related Accession (Same CDS sequence)	XM_001462721.1
Accession Version	XM_001462721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	ATP-dependent Clp protease subunit, heat shock protein 78 (HSP78)
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009387). COMPLETENESS: incomplete on both ends.

ATGCTGCGCA GGCGTCTCGT TCAGGCCTCC ACGGCGCTTC GGTGTGGCAG CACCGCTGCC 
CTGGCGGCTC CCTTCGGCCC TTTCGGGTGC GGCGGCCCCC GCAAAACTGC CGTGGCTGCT 
GTGGCGTGCT CGCCGTTGGC ATCGAGCACG GCGGCACACC CTACGTTACG GACGAGCTGC 
CGATGGCAGT CGACAGGCGT CACGCCGTGG AATAGCAGCG GCAACAGCGG TGGAGGCGGC 
AACGGCGGCC AGCAGCAGGG ACGCACGTGG GTGAACCCGA ACGCTGTGCC GCCGGGCGAG 
TTCCTAAAGA AGTACGCGCG CGACTTGACC GAGGAGGCGC GCATGGGTCG CCTGGACCCC 
ATCGTGGGGC GCGAGGAGGT CATTCGACGC ACCATCCAGG TCGTCTCGCG GCGTACCAAG 
AACAACCCGG TGCTGATTGG TGAGCCCGGC GTCGGTAAGA CGGCCATCGT CGAAGGTCTG 
GCGCAGCGGA TCGTGAAGGG CGAGGTGCCA GAGAGCATCA AGGACCGCCA GGTGTACGCG 
CTAGACATGG GCACCCTCGT CGCCGGCGCC AAGTTCCGCG GCGAGTTCGA GGAGCGCCTC 
AAGGGGGTGC TCAAGGACAC CATACAGTCG CACGGCAAGG TTATCTTGTT TATCGACGAG 
CTGCATACGT TGGTCGGCGC CGGCTCCTCC GGCGATGGGT CGATGGACGC GGCGAACCTG 
CTGAAGCCGT CACTGGCGCG CGGCGAGCTG CACTGCATCG GCGCCACCAC CTTGGACGAG 
TACCGCCAGC ACATCGAGAA GGACGCCGCG CTGGCGCGCC GCTTCCAGTC GGTGCTGGTT 
ACGGAGCCCA CTGTGGAAGA GACCATCTCC ATTCTGCGCG GCATCAAGGA GAAGTACGAG 
GCCCACCACG GCTGCCTCAT CAAGGACGAG GCCCTCGTCT ACGCCGCCGT CAACTCCCAC 
CGCTACCTCT CGGAGCGTCG CCTGCCAGAC AAGGCCATCG ACCTTATCGA CGAGGCCGCT 
AGCCGCCTGC GCCTGCAGCA GGAGTCGAAG CCAGAGGCGC TCGACCTCAT CGAGCGCGAG 
CTGATCCGCC TGAAGATCGA GGCGGAGGCG GTGAAGAAGG ATAAGGACGA GGTGGGCAAG 
GAGAAGCTGG AGAACTTGTA CGAGCAAATT TCGCAGAAGC AGAAGGAGTA CGACGCACTC 
GAGGAGCGGT GGAAGAAGGA GAAGAACCTG TTCCAGACGA TCAAGCAGCG CACCGAGGAA 
CTCGACGTGC TGCGGCACCA CCTCGAGCAG GCGATGAACA GCGGCGACTT TGCCAAGGCG 
GGCGAGATAC AGCACAGCCA AATCCCCAAC CTGCTGAAGC AGATCGAGAA GGACAAGCAC 
CTCGCCAGCT CCAAGAACTT CATGGTGCAC GACAGCGTCA CCTCAAAGGA CATTGCTGAG 
GTGATTGCCC GCGCGACCGG CATCCCCGTG GCGCAGCTCA TGACGAGTGA GCGGGAGAAG 
CTCATTCACA TGGATGCGGA GCTAAAGAAG ACGATCATGG GCCAGGATGC GGCTATCGAG 
TCCATTACGA ATGTGGTGCG CATCTCTCGC GCCGGCCTGC ACTCGCACAA GCGCCCGCTA 
GGCTCCTTTC TGTTCCTCGG CCCCACCGGC GTCGGTAAGA CGGAGGTGTG CAAGAGCCTC 
GCCAAGTTCC TCTTCGACGA CGAGTCGTTC ATCTGCCGCA TCGACATGAG CGAGTACATG 
GAACGGCACT CGGTGCACCG GCTGATCGGC GCCCCACCAG GGTACGTCGG CTATGAGGAA 
GGCGGCGAGC TGACGGAATC GGTGCGGCGT CGCCCTTACC AAATCGTCCT CTTCGACGAG 
TTCGAGAAGG CCCACCCGAG CGTGTCGAAC ATTCTTCTCC AAGTGCTGGA CGAGGGGCAC 
CTGACCGACA GCCACGGCCG CCGCGTCGAC TTCAAGAACA CCATCATCAT CCTGACCTCC 
AACATCGGCG CTGACGTCAT CGCCCGCCTG CCGGAGGGGG AGCCGAGCAT CAGTGCGATG 
CCGGCTGTCA TGGAGCAGGT GCGGCAGCGC CTGGCGCCCG AGTTCATTAA CCGTCTCGAC 
GACATCATCA TGTTTAACCG CCTCAGCCGT GAGGACATCC GCAGCATCGT GGAGATTTTG 
TTCGCACAGG TGCAGAAAAT GCTGACGGAG CATAACATCA CATTGGAAGA GTCCACGGAG 
GTGTACGACT GGCTCAGCGT CAACGGCTAC AGCGCTGTGT ATGGTGCGCG GCCCCTGAAG 
CGTCTCGTGC AGAGCGAACT GCTCAACCAA CTGGCGCTGA TGTTGCTTGA TGGCCGCATC 
CGTGAGGGTG AGCTTGTGAG GCTGTCTGTG CAGGACGGTC ATGTCGTGGT GGTTGCCAAT 
CACGCGCTCT CTCCTGAGAA GGGGCCGACT GACGCGCGAC TGCTGGATCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001462758.1
CDS	1..2454
Translation	MLRRRLVQASTALRCGSTAALAAPFGPFGCGGPRKTAVAAVACSPLASSTAAHPTLRTSCRWQSTGVTPWNSSGNSGGGGNGGQQQGRTWVNPNAVPPGEFLKKYARDLTEEARMGRLDPIVGREEVIRRTIQVVSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVKGEVPESIKDRQVYALDMGTLVAGAKFRGEFEERLKGVLKDTIQSHGKVILFIDELHTLVGAGSSGDGSMDAANLLKPSLARGELHCIGATTLDEYRQHIEKDAALARRFQSVLVTEPTVEETISILRGIKEKYEAHHGCLIKDEALVYAAVNSHRYLSERRLPDKAIDLIDEAASRLRLQQESKPEALDLIERELIRLKIEAEAVKKDKDEVGKEKLENLYEQISQKQKEYDALEERWKKEKNLFQTIKQRTEELDVLRHHLEQAMNSGDFAKAGEIQHSQIPNLLKQIEKDKHLASSKNFMVHDSVTSKDIAEVIARATGIPVAQLMTSEREKLIHMDAELKKTIMGQDAAIESITNVVRISRAGLHSHKRPLGSFLFLGPTGVGKTEVCKSLAKFLFDDESFICRIDMSEYMERHSVHRLIGAPPGYVGYEEGGELTESVRRRPYQIVLFDEFEKAHPSVSNILLQVLDEGHLTDSHGRRVDFKNTIIILTSNIGADVIARLPEGEPSISAMPAVMEQVRQRLAPEFINRLDDIIMFNRLSREDIRSIVEILFAQVQKMLTEHNITLEESTEVYDWLSVNGYSAVYGARPLKRLVQSELLNQLALMLLDGRIREGELVRLSVQDGHVVVVANHALSPEKGPTDARLLDQ

Target ORF information:

RefSeq Version	XM_001462721.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 ATP-dependent Clp protease subunit, heat shock protein 78 (HSP78) partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001462721.1

ORF Insert Sequence:

ATGCTGCGCA GGCGTCTCGT TCAGGCCTCC ACGGCGCTTC GGTGTGGCAG CACCGCTGCC 
CTGGCGGCTC CCTTCGGCCC TTTCGGGTGC GGCGGCCCCC GCAAAACTGC CGTGGCTGCT 
GTGGCGTGCT CGCCGTTGGC ATCGAGCACG GCGGCACACC CTACGTTACG GACGAGCTGC 
CGATGGCAGT CGACAGGCGT CACGCCGTGG AATAGCAGCG GCAACAGCGG TGGAGGCGGC 
AACGGCGGCC AGCAGCAGGG ACGCACGTGG GTGAACCCGA ACGCTGTGCC GCCGGGCGAG 
TTCCTAAAGA AGTACGCGCG CGACTTGACC GAGGAGGCGC GCATGGGTCG CCTGGACCCC 
ATCGTGGGGC GCGAGGAGGT CATTCGACGC ACCATCCAGG TCGTCTCGCG GCGTACCAAG 
AACAACCCGG TGCTGATTGG TGAGCCCGGC GTCGGTAAGA CGGCCATCGT CGAAGGTCTG 
GCGCAGCGGA TCGTGAAGGG CGAGGTGCCA GAGAGCATCA AGGACCGCCA GGTGTACGCG 
CTAGACATGG GCACCCTCGT CGCCGGCGCC AAGTTCCGCG GCGAGTTCGA GGAGCGCCTC 
AAGGGGGTGC TCAAGGACAC CATACAGTCG CACGGCAAGG TTATCTTGTT TATCGACGAG 
CTGCATACGT TGGTCGGCGC CGGCTCCTCC GGCGATGGGT CGATGGACGC GGCGAACCTG 
CTGAAGCCGT CACTGGCGCG CGGCGAGCTG CACTGCATCG GCGCCACCAC CTTGGACGAG 
TACCGCCAGC ACATCGAGAA GGACGCCGCG CTGGCGCGCC GCTTCCAGTC GGTGCTGGTT 
ACGGAGCCCA CTGTGGAAGA GACCATCTCC ATTCTGCGCG GCATCAAGGA GAAGTACGAG 
GCCCACCACG GCTGCCTCAT CAAGGACGAG GCCCTCGTCT ACGCCGCCGT CAACTCCCAC 
CGCTACCTCT CGGAGCGTCG CCTGCCAGAC AAGGCCATCG ACCTTATCGA CGAGGCCGCT 
AGCCGCCTGC GCCTGCAGCA GGAGTCGAAG CCAGAGGCGC TCGACCTCAT CGAGCGCGAG 
CTGATCCGCC TGAAGATCGA GGCGGAGGCG GTGAAGAAGG ATAAGGACGA GGTGGGCAAG 
GAGAAGCTGG AGAACTTGTA CGAGCAAATT TCGCAGAAGC AGAAGGAGTA CGACGCACTC 
GAGGAGCGGT GGAAGAAGGA GAAGAACCTG TTCCAGACGA TCAAGCAGCG CACCGAGGAA 
CTCGACGTGC TGCGGCACCA CCTCGAGCAG GCGATGAACA GCGGCGACTT TGCCAAGGCG 
GGCGAGATAC AGCACAGCCA AATCCCCAAC CTGCTGAAGC AGATCGAGAA GGACAAGCAC 
CTCGCCAGCT CCAAGAACTT CATGGTGCAC GACAGCGTCA CCTCAAAGGA CATTGCTGAG 
GTGATTGCCC GCGCGACCGG CATCCCCGTG GCGCAGCTCA TGACGAGTGA GCGGGAGAAG 
CTCATTCACA TGGATGCGGA GCTAAAGAAG ACGATCATGG GCCAGGATGC GGCTATCGAG 
TCCATTACGA ATGTGGTGCG CATCTCTCGC GCCGGCCTGC ACTCGCACAA GCGCCCGCTA 
GGCTCCTTTC TGTTCCTCGG CCCCACCGGC GTCGGTAAGA CGGAGGTGTG CAAGAGCCTC 
GCCAAGTTCC TCTTCGACGA CGAGTCGTTC ATCTGCCGCA TCGACATGAG CGAGTACATG 
GAACGGCACT CGGTGCACCG GCTGATCGGC GCCCCACCAG GGTACGTCGG CTATGAGGAA 
GGCGGCGAGC TGACGGAATC GGTGCGGCGT CGCCCTTACC AAATCGTCCT CTTCGACGAG 
TTCGAGAAGG CCCACCCGAG CGTGTCGAAC ATTCTTCTCC AAGTGCTGGA CGAGGGGCAC 
CTGACCGACA GCCACGGCCG CCGCGTCGAC TTCAAGAACA CCATCATCAT CCTGACCTCC 
AACATCGGCG CTGACGTCAT CGCCCGCCTG CCGGAGGGGG AGCCGAGCAT CAGTGCGATG 
CCGGCTGTCA TGGAGCAGGT GCGGCAGCGC CTGGCGCCCG AGTTCATTAA CCGTCTCGAC 
GACATCATCA TGTTTAACCG CCTCAGCCGT GAGGACATCC GCAGCATCGT GGAGATTTTG 
TTCGCACAGG TGCAGAAAAT GCTGACGGAG CATAACATCA CATTGGAAGA GTCCACGGAG 
GTGTACGACT GGCTCAGCGT CAACGGCTAC AGCGCTGTGT ATGGTGCGCG GCCCCTGAAG 
CGTCTCGTGC AGAGCGAACT GCTCAACCAA CTGGCGCTGA TGTTGCTTGA TGGCCGCATC 
CGTGAGGGTG AGCTTGTGAG GCTGTCTGTG CAGGACGGTC ATGTCGTGGT GGTTGCCAAT 
CACGCGCTCT CTCCTGAGAA GGGGCCGACT GACGCGCGAC TGCTGGATCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_02_0680 cDNA ORF clone, Leishmania infantum JPCM5

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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