LOC101074686 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101074686
Entrez Gene ID	101074686
Full Name	toll like receptor 7
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011609151.1	XP_011607453.1	toll-like receptor 7
XM_011609151.2	XP_011607453.2	toll-like receptor 7

Pathways from BioSystems
Gene Ontology

The following LOC101074686 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101074686 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo28468	XM_011609151.1 Latest version!	Takifugu rubripes toll-like receptor 7 (tlr7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo42697	XM_011609151.2 Latest version!	Takifugu rubripes toll-like receptor 7 (LOC101074686), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101074686 ( XM_011609151.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011607453.1 Takifugu rubripes toll-like receptor 7 (tlr7), mRNA.

Price & Availability↑

CloneID	OTo28468
Clone ID Related Accession (Same CDS sequence)	XM_011609151.1
Accession Version	XM_011609151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	toll-like receptor 7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070905.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTCC TCTTGATTTT GGGCCTTTGG TGCCCTCTCT GCATACTGAC AGCTAATGTT 
TCATACCCCA AAACACTGCC ATGTGACGTG ACCAACAACG GGAGCGTGGT GAATGTGGAC 
TGCACCGAGC GAAGTCTCAA AGGTGTCCCC GTTGGCATCC CCAGAGACGT CACCAATCTG 
ACACTCACCA TCAACCATAT TCCTAATTTC AACTCCAGCT CCTTCCAAGG CCTGGACAAC 
CTGAGGGAGA TTGACATGAG GTGCAACTGC GTGCCCGTTA AAATCGGCCC CAAGGACCGT 
ATTTGCACAG AGAGCGTGAC GATAGAGGAG AATACCTTCA CCAGCCTGAG GAATCTGCAG 
TCGCTTTATT TAGACGGCAA TCAGCTGTAC ATTATACCTG AAGGCCTGCC CTCCAGTCTC 
ATCCTTCTGA GTTTAGAAGT GAATCACATT TATTATATTT CAAAAGCAAA CCTCTCTGAG 
ATCAGAAACA TTGAGAGTCT TTACCTGGGT CAAAATTGTT ACTATCGTAA CCCCTGTAAT 
TTTTCCTATG GCATAGAGGA GGGTGCTTTT CTGGAGCTCC ACAATTTAAA ACTGCTCAGT 
CTTAAATCAA ACAACCTCTC CTTTATTCCA CATCACCTCC CCTCCAGTCT TAAGGAGCTG 
TATCTCTACA ACAACAACTT TCAACAAGTC ACCGATGAGG ATTTTAAAAA CCTAACAAAC 
CTTGAGATCC TGGACATTAG TGGAAACTGT CCCCGCTGTT ACAATGTCCC CTTCCCATGC 
AACCCATGTC CCAATAACTC GCCGCTTCAA ATCAGCAAGT CAGCCTTTAA AACACTGACG 
AAACTGAAGA CATTGCGCAT GCACAGTAAC TCTCTCAGGT ATGTCGTACC CGAGTGGTTT 
GCCAGCACAA AGGAGCTCAG AGTGCTCGAT CTGTCCTCAA ATCATTTAGC CAGAGACATA 
GGAGTCACTC TGTTCCCACA TCATCTGGGC AAACTGCAAG AACTGGATCT TTCCTTTAAC 
TATGAGCTGC AGAGGTACCC TCGGACGCTG AGACTGAGCT GCAGTTTTAA ATCTCTGAAT 
TCTCTAAGAA TTCTCAGGCT CCAAGGGTTT GTCTTTCAGC AGTTAAAGCC AGAGAGCATC 
ACTCCCTTAA AAAGCCTGAC AAATTTGGAG GTCCTGGATC TTGGCACGAA TTTCATCAAA 
ATGACAAACC TCAGTATCCT GATGGAGTTG AAGAGCTTCA AAATAATCAA TCTCTCCAAC 
AATAGAATAT CGTCCCCTTC TGATGGACAA GCGGCCGTTG GTTTCTCAGG GGGACAGGCC 
TGGGAATGGT CTCCCATGTT GCCTGCTGAT CAGTACCAAA ATGAGGAAGT GAGAGAGATC 
CATTACTTCA GATATGATGA ATACGCACGC AGCTGCAAAT ATAAAGACAA AGAACTCGGA 
GTCATTGCAT CCTTTGTGAA GAGGGAATGC AGCCAGTTTG GAAAAACCCT GGACGTGAGC 
AGAAATAACA TATTCTTCTT ACATTCCAGG TTTTTAAATC TCAGAGAGCT GAAGTGTCTT 
AACCTGTCTG GAAATGCAAT GAGCCAAAGC CTGAATGGCT CTGAATTCAC CTATCTGACA 
GGTTTGCAGT ATCTGGATTT CTCTTCAAAT CGCTTGGACC TGCTCTACTC CACCGCCTTC 
CAAGAGCTGA AAAATCTGGT CATCTTAGAT ATTAGTAACA ACAACCATTA TTTTGAATCT 
GAGGGCTTGA CTCACATGCT TAATTTCACC CAAAACCTGA AAAATCTCAA AATACTGATG 
ATGAACCACA ACAAAATCTC CACTTCCACT AACACGGAGC TGGAGAGCCA GTCTCTGGAG 
AGGTTGGAGT TCAGAGGCAA TCGCTTAGAT ATGTTGTGGA GAGATGGCAA CACAAGATAT 
CTTAATTATT TTAAGAAATT ATCCAATCTG GCTGTCCTTG ATATCTCACA TAACAACCTG 
AATTCCATTC AACAGCAAGT GTTTAGCGGC TTGCCAGACA CACTCTCTGA GCTCTATGTG 
AATAACAACA AACTTAAATC CTTTGCTTGG GGAGAACTTT CGCTCTTGCC TTCTCTGCTC 
GTCTTAGATC TCAGTGGAAA CTACTTAACT ACAGTTCCAC ACATGCTGTC AAACTGCTCC 
AAATCTCTCA AAAAGCTCAT TTTACATAAG AACGAAATCC TAAAGCTGAC TCCAGATTTC 
CTTAAGTATG CCTACAACTT GAAATATCTG GATCTGAGTT TTAATCGCAT AGGGCAAATT 
GAGAAATCAA ATTTCCCAGA TGACGTTGTA AATGGGATGG ACATGCTGCT TCTTCATAAA 
AACAGATTCC AGTGCAGCTG CAGTGCTATT TGGTTTGTCA TGTGGCTCAA CCAGACTACA 
GTGACCATCC CCAGACTCGC TACAGATGTG ACCTGTGCTT CTCCAGGGGC ACAGAGGGGT 
CATCCAGTGA TCTCAGTGGA TCTGTTGGCC TGTCAGCACA ACTTCCTGTC AATCATTCTC 
TACTTTCTCA TGACTTCCTT CACCCTGATT TTCTTCACAC TGGCCATCTC CAGTCACCTC 
TTCCTGTGGG ACGTCTGGTA CATCTACCAC TTCTGCAGGG CCAAGCTCAA GGGCTACAGT 
CAACTCTCCT CTCAAAGCTC TGTCTACGAT GCATTTGTGA TGTATGACAA AGAGGATTCT 
GCGGTGTCGG AGTGGGTGAT GAAGGAAATG TGCGTGCAGC TGGAGGGTCG AGAAGACCGG 
CGTCTGACGC TGTGTCTGGA GGAACGGGAC TGGATACCCG GCACTCCCGT CATCGATAAC 
CTTTCCCAGA GCATCCACAG AAGCAAGAGG ACTGTATTCA TTCTTACAAA CACATACATC 
AAAAGCGGCA GCTTCAAAAC CGCCTTCTAC ATGGCCCACC AAAGGCTAAT GGATGAAAAG 
AACGATGTTA TTGTTCTGAT CTTCCTGGAG AACATACCTT ATAATTCCAG GTATCTGAGA 
CTGAGGAGAA GACTGTATAG ACGGTCTGTG CTGGAGTGGC CGACGAACCC TCAGGCCCGT 
CCATATTTCT GGTTCAGCGT GAGAAGTGTG TTGGCAACAG AAAGCCACAA ACAATACAAC 
AACCTCTTTA AAGAGACACT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011607453.1
CDS	924..4067
Translation	MFLLLILGLWCPLCILTANVSYPKTLPCDVTNNGSVVNVDCTERSLKGVPVGIPRDVTNLTLTINHIPNFNSSSFQGLDNLREIDMRCNCVPVKIGPKDRICTESVTIEENTFTSLRNLQSLYLDGNQLYIIPEGLPSSLILLSLEVNHIYYISKANLSEIRNIESLYLGQNCYYRNPCNFSYGIEEGAFLELHNLKLLSLKSNNLSFIPHHLPSSLKELYLYNNNFQQVTDEDFKNLTNLEILDISGNCPRCYNVPFPCNPCPNNSPLQISKSAFKTLTKLKTLRMHSNSLRYVVPEWFASTKELRVLDLSSNHLARDIGVTLFPHHLGKLQELDLSFNYELQRYPRTLRLSCSFKSLNSLRILRLQGFVFQQLKPESITPLKSLTNLEVLDLGTNFIKMTNLSILMELKSFKIINLSNNRISSPSDGQAAVGFSGGQAWEWSPMLPADQYQNEEVREIHYFRYDEYARSCKYKDKELGVIASFVKRECSQFGKTLDVSRNNIFFLHSRFLNLRELKCLNLSGNAMSQSLNGSEFTYLTGLQYLDFSSNRLDLLYSTAFQELKNLVILDISNNNHYFESEGLTHMLNFTQNLKNLKILMMNHNKISTSTNTELESQSLERLEFRGNRLDMLWRDGNTRYLNYFKKLSNLAVLDISHNNLNSIQQQVFSGLPDTLSELYVNNNKLKSFAWGELSLLPSLLVLDLSGNYLTTVPHMLSNCSKSLKKLILHKNEILKLTPDFLKYAYNLKYLDLSFNRIGQIEKSNFPDDVVNGMDMLLLHKNRFQCSCSAIWFVMWLNQTTVTIPRLATDVTCASPGAQRGHPVISVDLLACQHNFLSIILYFLMTSFTLIFFTLAISSHLFLWDVWYIYHFCRAKLKGYSQLSSQSSVYDAFVMYDKEDSAVSEWVMKEMCVQLEGREDRRLTLCLEERDWIPGTPVIDNLSQSIHRSKRTVFILTNTYIKSGSFKTAFYMAHQRLMDEKNDVIVLIFLENIPYNSRYLRLRRRLYRRSVLEWPTNPQARPYFWFSVRSVLATESHKQYNNLFKETL

Target ORF information:

RefSeq Version	XM_011609151.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes toll-like receptor 7 (tlr7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609151.1

ORF Insert Sequence:

ATGTTTCTCC TCTTGATTTT GGGCCTTTGG TGCCCTCTCT GCATACTGAC AGCTAATGTT 
TCATACCCCA AAACACTGCC ATGTGACGTG ACCAACAACG GGAGCGTGGT GAATGTGGAC 
TGCACCGAGC GAAGTCTCAA AGGTGTCCCC GTTGGCATCC CCAGAGACGT CACCAATCTG 
ACACTCACCA TCAACCATAT TCCTAATTTC AACTCCAGCT CCTTCCAAGG CCTGGACAAC 
CTGAGGGAGA TTGACATGAG GTGCAACTGC GTGCCCGTTA AAATCGGCCC CAAGGACCGT 
ATTTGCACAG AGAGCGTGAC GATAGAGGAG AATACCTTCA CCAGCCTGAG GAATCTGCAG 
TCGCTTTATT TAGACGGCAA TCAGCTGTAC ATTATACCTG AAGGCCTGCC CTCCAGTCTC 
ATCCTTCTGA GTTTAGAAGT GAATCACATT TATTATATTT CAAAAGCAAA CCTCTCTGAG 
ATCAGAAACA TTGAGAGTCT TTACCTGGGT CAAAATTGTT ACTATCGTAA CCCCTGTAAT 
TTTTCCTATG GCATAGAGGA GGGTGCTTTT CTGGAGCTCC ACAATTTAAA ACTGCTCAGT 
CTTAAATCAA ACAACCTCTC CTTTATTCCA CATCACCTCC CCTCCAGTCT TAAGGAGCTG 
TATCTCTACA ACAACAACTT TCAACAAGTC ACCGATGAGG ATTTTAAAAA CCTAACAAAC 
CTTGAGATCC TGGACATTAG TGGAAACTGT CCCCGCTGTT ACAATGTCCC CTTCCCATGC 
AACCCATGTC CCAATAACTC GCCGCTTCAA ATCAGCAAGT CAGCCTTTAA AACACTGACG 
AAACTGAAGA CATTGCGCAT GCACAGTAAC TCTCTCAGGT ATGTCGTACC CGAGTGGTTT 
GCCAGCACAA AGGAGCTCAG AGTGCTCGAT CTGTCCTCAA ATCATTTAGC CAGAGACATA 
GGAGTCACTC TGTTCCCACA TCATCTGGGC AAACTGCAAG AACTGGATCT TTCCTTTAAC 
TATGAGCTGC AGAGGTACCC TCGGACGCTG AGACTGAGCT GCAGTTTTAA ATCTCTGAAT 
TCTCTAAGAA TTCTCAGGCT CCAAGGGTTT GTCTTTCAGC AGTTAAAGCC AGAGAGCATC 
ACTCCCTTAA AAAGCCTGAC AAATTTGGAG GTCCTGGATC TTGGCACGAA TTTCATCAAA 
ATGACAAACC TCAGTATCCT GATGGAGTTG AAGAGCTTCA AAATAATCAA TCTCTCCAAC 
AATAGAATAT CGTCCCCTTC TGATGGACAA GCGGCCGTTG GTTTCTCAGG GGGACAGGCC 
TGGGAATGGT CTCCCATGTT GCCTGCTGAT CAGTACCAAA ATGAGGAAGT GAGAGAGATC 
CATTACTTCA GATATGATGA ATACGCACGC AGCTGCAAAT ATAAAGACAA AGAACTCGGA 
GTCATTGCAT CCTTTGTGAA GAGGGAATGC AGCCAGTTTG GAAAAACCCT GGACGTGAGC 
AGAAATAACA TATTCTTCTT ACATTCCAGG TTTTTAAATC TCAGAGAGCT GAAGTGTCTT 
AACCTGTCTG GAAATGCAAT GAGCCAAAGC CTGAATGGCT CTGAATTCAC CTATCTGACA 
GGTTTGCAGT ATCTGGATTT CTCTTCAAAT CGCTTGGACC TGCTCTACTC CACCGCCTTC 
CAAGAGCTGA AAAATCTGGT CATCTTAGAT ATTAGTAACA ACAACCATTA TTTTGAATCT 
GAGGGCTTGA CTCACATGCT TAATTTCACC CAAAACCTGA AAAATCTCAA AATACTGATG 
ATGAACCACA ACAAAATCTC CACTTCCACT AACACGGAGC TGGAGAGCCA GTCTCTGGAG 
AGGTTGGAGT TCAGAGGCAA TCGCTTAGAT ATGTTGTGGA GAGATGGCAA CACAAGATAT 
CTTAATTATT TTAAGAAATT ATCCAATCTG GCTGTCCTTG ATATCTCACA TAACAACCTG 
AATTCCATTC AACAGCAAGT GTTTAGCGGC TTGCCAGACA CACTCTCTGA GCTCTATGTG 
AATAACAACA AACTTAAATC CTTTGCTTGG GGAGAACTTT CGCTCTTGCC TTCTCTGCTC 
GTCTTAGATC TCAGTGGAAA CTACTTAACT ACAGTTCCAC ACATGCTGTC AAACTGCTCC 
AAATCTCTCA AAAAGCTCAT TTTACATAAG AACGAAATCC TAAAGCTGAC TCCAGATTTC 
CTTAAGTATG CCTACAACTT GAAATATCTG GATCTGAGTT TTAATCGCAT AGGGCAAATT 
GAGAAATCAA ATTTCCCAGA TGACGTTGTA AATGGGATGG ACATGCTGCT TCTTCATAAA 
AACAGATTCC AGTGCAGCTG CAGTGCTATT TGGTTTGTCA TGTGGCTCAA CCAGACTACA 
GTGACCATCC CCAGACTCGC TACAGATGTG ACCTGTGCTT CTCCAGGGGC ACAGAGGGGT 
CATCCAGTGA TCTCAGTGGA TCTGTTGGCC TGTCAGCACA ACTTCCTGTC AATCATTCTC 
TACTTTCTCA TGACTTCCTT CACCCTGATT TTCTTCACAC TGGCCATCTC CAGTCACCTC 
TTCCTGTGGG ACGTCTGGTA CATCTACCAC TTCTGCAGGG CCAAGCTCAA GGGCTACAGT 
CAACTCTCCT CTCAAAGCTC TGTCTACGAT GCATTTGTGA TGTATGACAA AGAGGATTCT 
GCGGTGTCGG AGTGGGTGAT GAAGGAAATG TGCGTGCAGC TGGAGGGTCG AGAAGACCGG 
CGTCTGACGC TGTGTCTGGA GGAACGGGAC TGGATACCCG GCACTCCCGT CATCGATAAC 
CTTTCCCAGA GCATCCACAG AAGCAAGAGG ACTGTATTCA TTCTTACAAA CACATACATC 
AAAAGCGGCA GCTTCAAAAC CGCCTTCTAC ATGGCCCACC AAAGGCTAAT GGATGAAAAG 
AACGATGTTA TTGTTCTGAT CTTCCTGGAG AACATACCTT ATAATTCCAG GTATCTGAGA 
CTGAGGAGAA GACTGTATAG ACGGTCTGTG CTGGAGTGGC CGACGAACCC TCAGGCCCGT 
CCATATTTCT GGTTCAGCGT GAGAAGTGTG TTGGCAACAG AAAGCCACAA ACAATACAAC 
AACCTCTTTA AAGAGACACT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101074686 ( XM_011609151.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011607453.2 Takifugu rubripes toll-like receptor 7 (LOC101074686), mRNA.

Price & Availability↑

CloneID	OTo42697
Clone ID Related Accession (Same CDS sequence)	XM_011609151.2
Accession Version	XM_011609151.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	toll-like receptor 7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011609151.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTCC TCTTGATTTT GGGCCTTTGG TGCCCTCTCT GCATACTGAC AGCTAATGTT 
TCATACCCCA AAACACTGCC ATGTGACGTG ACCAACAACG GGAGCGTGGT GAATGTGGAC 
TGCACCGAGC GAAGTCTCAA AGGTGTCCCC GTTGGCATCC CCAGAGACGT CACCAATCTG 
ACACTCACCA TCAACCATAT TCCTAATTTC AACTCCAGCT CCTTCCAAGG CCTGGACAAC 
CTGAGGGAGA TTGACATGAG GTGCAACTGC GTGCCCGTTA AAATCGGCCC CAAGGACCGT 
ATTTGCACAG AGAGCGTGAC GATAGAGGAG AATACCTTCA CCAGCCTGAG GAATCTGCAG 
TCGCTTTATT TAGACGGCAA TCAGCTGTAC ATTATACCTG AAGGCCTGCC CTCCAGTCTC 
ATCCTTCTGA GTTTAGAAGT GAATCACATT TATTACATTT CAAAAGCAAA CCTCTCTGAG 
ATCAGAAACA TTGAGAGTCT TTACCTGGGT CAAAATTGTT ACTATCGTAA CCCCTGTAAT 
TTTTCCTATG GCATAGAGGA GGGTGCTTTT CTGGAGCTCC ACAATTTAAA ACTGCTCAGT 
CTTAAATCAA ACAACCTCTC CTTTATTCCA CATCACCTCC CCTCCAGTCT TAAGGAGCTG 
TATCTCTACA ACAACAACTT TCAACAAGTC ACCGATGAGG ATTTTAAAAA CCTAACAAAC 
CTTGAGATCC TGGACATTAG TGGAAACTGT CCCCGCTGTT ACAATGTCCC CTTCCCATGC 
AACCCATGTC CCAATAACTC GCCGCTTCAA ATCAGCAAGT CAGCCTTTAA AACACTGACG 
AAACTGAAGA CATTGCGCAT GCACAGTAAC TCTCTCAGGT ATGTCGTACC CGAGTGGTTT 
GCCAGCACAA AGGAGCTCAG AGTGCTCGAT CTGTCCTCAA ATCATTTAGC CAGAGACATA 
GGAGTCACTC TGTTCCCACA TCATCTGGGC AAACTGCAAG AACTGGATCT TTCCTTTAAC 
TATGAGCTGC AGAGGTACCC TCGGACGCTG AGACTGAGCT GCAGTTTTAA ATCTCTGAAT 
TCTCTAAGAA TTCTCAGGCT CCAAGGGTTT GTCTTTCAGC AGTTAAAGCC AGAGAGCATC 
ACTCCCTTAA AAAGCCTGAC AAATTTGGAG GTCCTGGATC TTGGCACGAA TTTCATCAAA 
ATGACAAACC TCAGTATCCT GATGGAGTTG AAGAGCTTCA AAATAATCAA TCTCTCCAAC 
AATAGAATAT CGTCCCCTTC TGATGGACAA GCGGCCGTTG GTTTCTCAGG GGGACAGGCC 
TGGGAATGGT CTCCCATGTT GCCTGCTGAT CAGTACCAAA ATGAGGAAGT GAGAGAGATC 
CATTACTTCA GATATGATGA ATACGCACGC AGCTGCAAAT ATAAAGACAA AGAACTCGGA 
GTCATTGCAT CCTTTGTGAA GAGGGAATGC AGCCAGTTTG GAAAAACCCT GGACGTGAGC 
AGAAATAACA TATTCTTCTT ACATTCCAGG TTTTTAAATC TCAGAGAGCT GAAGTGTCTT 
AACCTGTCTG GAAATGCAAT GAGCCAAAGC CTGAATGGCT CTGAATTCAC CTATCTGACA 
GGTTTGCAGT ATCTGGATTT CTCTTCAAAT CGCTTGGACC TGCTCTACTC CACCGCCTTC 
CAAGAGCTGA AAAATCTGGT CATCTTAGAT ATTAGTAACA ACAACCATTA TTTTGAATCT 
GAGGGCTTGA CTCACATGCT TAATTTCACC CAAAACCTGA AAAATCTCAA AATACTGATG 
ATGAACCACA ACAAAATCTC CACTTCCACT AACACGGAGC TGGAGAGCCA GTCTCTGGAG 
AGGTTGGAGT TCAGAGGCAA TCGCTTAGAT ATGTTGTGGA GAGATGGCAA CACAAGATAT 
CTTAATTATT TTAAGAAATT ATCCAATCTG GCTGTCCTTG ATATCTCACA TAACAACCTG 
AATTCCATTC AACAGCAAGT GTTTAGCGGC TTGCCAGACA CACTCTCTGA GCTCTATGTG 
AATAACAACA AACTTAAATC CTTTGCTTGG GGAGAACTTT CGCTCTTGCC TTCTCTGCTC 
GTCTTAGATC TCAGTGGAAA CTACTTAACT ACAGTTCCAC ACATGCTGTC AAACTGCTCC 
AAATCTCTCA AAAAGCTCAT TTTACATAAG AACGAAATCC TAAAGCTGAC TCCAGATTTC 
CTTAAGTATG CCTACAACTT GAAATATCTG GATCTGAGTT TTAATCGCAT AGGGCAAATT 
GAGAAATCAA ATTTCCCAGA TGACGTTGTA AATGGGATGG ACATGCTGCT TCTTCATAAA 
AACAGATTCC AGTGCAGCTG CAGTGCTATT TGGTTTGTCA TGTGGCTCAA CCAGACTACA 
GTGACCATCC CCAGACTCGC TACAGATGTG ACCTGTGCTT CTCCAGGGGC ACAGAGGGGT 
CATCCCGTGA TCTCAGTGGA TCTGTTGGCC TGTCAGCACA ACTTCCTGTC AATCATTCTC 
TACTTTCTCA TGACTTCCTT CACCCTGATT TTCTTCACAC TGGCCATCTC CAGTCACCTC 
TTCCTGTGGG ACGTCTGGTA CATCTACCAC TTCTGCAGGG CCAAGCTCAA GGGCTACAGT 
CAACTCTCCT CTCAAAGCTC TGTCTACGAT GCATTTGTGA TGTATGACAA AGAGGATTCT 
GCGGTGTCGG AGTGGGTGAT GAAGGAAATG TGCATGCAGC TGGAGGGTCG AGAAGACCGA 
CGTCTGACGC TGTGTCTGGA GGAACGGGAC TGGATACCCG GCACTCCCGT CATCGATAAC 
CTTTCCCAGA GCATCCACAG AAGCAAGAGG ACTGTATTCA TTCTTACAAA CACATACATC 
AAAAGCGGCA GCTTCAAAAC CGCCTTCTAC ATGGCCCACC AAAGGCTAAT GGATGAAAAG 
AACGATGTTA TTGTTCTGAT CTTCCTGGAG AACATACCTT ATAATTCCAG GTATCTGAGA 
CTGAGGAGAA GACTGTATAG ACGGTCTGTG CTGGAGTGGC CGACGAACCC TCAGGCCCGT 
CCATATTTCT GGTTCAGCGT GAGAAGTGTG TTGGCAACAG AAAGCCACAA ACAATACAAC 
AACCTCTTTA AAGAGACACT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011607453.2
CDS	105..3248
Translation	MFLLLILGLWCPLCILTANVSYPKTLPCDVTNNGSVVNVDCTERSLKGVPVGIPRDVTNLTLTINHIPNFNSSSFQGLDNLREIDMRCNCVPVKIGPKDRICTESVTIEENTFTSLRNLQSLYLDGNQLYIIPEGLPSSLILLSLEVNHIYYISKANLSEIRNIESLYLGQNCYYRNPCNFSYGIEEGAFLELHNLKLLSLKSNNLSFIPHHLPSSLKELYLYNNNFQQVTDEDFKNLTNLEILDISGNCPRCYNVPFPCNPCPNNSPLQISKSAFKTLTKLKTLRMHSNSLRYVVPEWFASTKELRVLDLSSNHLARDIGVTLFPHHLGKLQELDLSFNYELQRYPRTLRLSCSFKSLNSLRILRLQGFVFQQLKPESITPLKSLTNLEVLDLGTNFIKMTNLSILMELKSFKIINLSNNRISSPSDGQAAVGFSGGQAWEWSPMLPADQYQNEEVREIHYFRYDEYARSCKYKDKELGVIASFVKRECSQFGKTLDVSRNNIFFLHSRFLNLRELKCLNLSGNAMSQSLNGSEFTYLTGLQYLDFSSNRLDLLYSTAFQELKNLVILDISNNNHYFESEGLTHMLNFTQNLKNLKILMMNHNKISTSTNTELESQSLERLEFRGNRLDMLWRDGNTRYLNYFKKLSNLAVLDISHNNLNSIQQQVFSGLPDTLSELYVNNNKLKSFAWGELSLLPSLLVLDLSGNYLTTVPHMLSNCSKSLKKLILHKNEILKLTPDFLKYAYNLKYLDLSFNRIGQIEKSNFPDDVVNGMDMLLLHKNRFQCSCSAIWFVMWLNQTTVTIPRLATDVTCASPGAQRGHPVISVDLLACQHNFLSIILYFLMTSFTLIFFTLAISSHLFLWDVWYIYHFCRAKLKGYSQLSSQSSVYDAFVMYDKEDSAVSEWVMKEMCMQLEGREDRRLTLCLEERDWIPGTPVIDNLSQSIHRSKRTVFILTNTYIKSGSFKTAFYMAHQRLMDEKNDVIVLIFLENIPYNSRYLRLRRRLYRRSVLEWPTNPQARPYFWFSVRSVLATESHKQYNNLFKETL

Target ORF information:

RefSeq Version	XM_011609151.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes toll-like receptor 7 (LOC101074686), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609151.2

ORF Insert Sequence:

ATGTTTCTCC TCTTGATTTT GGGCCTTTGG TGCCCTCTCT GCATACTGAC AGCTAATGTT 
TCATACCCCA AAACACTGCC ATGTGACGTG ACCAACAACG GGAGCGTGGT GAATGTGGAC 
TGCACCGAGC GAAGTCTCAA AGGTGTCCCC GTTGGCATCC CCAGAGACGT CACCAATCTG 
ACACTCACCA TCAACCATAT TCCTAATTTC AACTCCAGCT CCTTCCAAGG CCTGGACAAC 
CTGAGGGAGA TTGACATGAG GTGCAACTGC GTGCCCGTTA AAATCGGCCC CAAGGACCGT 
ATTTGCACAG AGAGCGTGAC GATAGAGGAG AATACCTTCA CCAGCCTGAG GAATCTGCAG 
TCGCTTTATT TAGACGGCAA TCAGCTGTAC ATTATACCTG AAGGCCTGCC CTCCAGTCTC 
ATCCTTCTGA GTTTAGAAGT GAATCACATT TATTACATTT CAAAAGCAAA CCTCTCTGAG 
ATCAGAAACA TTGAGAGTCT TTACCTGGGT CAAAATTGTT ACTATCGTAA CCCCTGTAAT 
TTTTCCTATG GCATAGAGGA GGGTGCTTTT CTGGAGCTCC ACAATTTAAA ACTGCTCAGT 
CTTAAATCAA ACAACCTCTC CTTTATTCCA CATCACCTCC CCTCCAGTCT TAAGGAGCTG 
TATCTCTACA ACAACAACTT TCAACAAGTC ACCGATGAGG ATTTTAAAAA CCTAACAAAC 
CTTGAGATCC TGGACATTAG TGGAAACTGT CCCCGCTGTT ACAATGTCCC CTTCCCATGC 
AACCCATGTC CCAATAACTC GCCGCTTCAA ATCAGCAAGT CAGCCTTTAA AACACTGACG 
AAACTGAAGA CATTGCGCAT GCACAGTAAC TCTCTCAGGT ATGTCGTACC CGAGTGGTTT 
GCCAGCACAA AGGAGCTCAG AGTGCTCGAT CTGTCCTCAA ATCATTTAGC CAGAGACATA 
GGAGTCACTC TGTTCCCACA TCATCTGGGC AAACTGCAAG AACTGGATCT TTCCTTTAAC 
TATGAGCTGC AGAGGTACCC TCGGACGCTG AGACTGAGCT GCAGTTTTAA ATCTCTGAAT 
TCTCTAAGAA TTCTCAGGCT CCAAGGGTTT GTCTTTCAGC AGTTAAAGCC AGAGAGCATC 
ACTCCCTTAA AAAGCCTGAC AAATTTGGAG GTCCTGGATC TTGGCACGAA TTTCATCAAA 
ATGACAAACC TCAGTATCCT GATGGAGTTG AAGAGCTTCA AAATAATCAA TCTCTCCAAC 
AATAGAATAT CGTCCCCTTC TGATGGACAA GCGGCCGTTG GTTTCTCAGG GGGACAGGCC 
TGGGAATGGT CTCCCATGTT GCCTGCTGAT CAGTACCAAA ATGAGGAAGT GAGAGAGATC 
CATTACTTCA GATATGATGA ATACGCACGC AGCTGCAAAT ATAAAGACAA AGAACTCGGA 
GTCATTGCAT CCTTTGTGAA GAGGGAATGC AGCCAGTTTG GAAAAACCCT GGACGTGAGC 
AGAAATAACA TATTCTTCTT ACATTCCAGG TTTTTAAATC TCAGAGAGCT GAAGTGTCTT 
AACCTGTCTG GAAATGCAAT GAGCCAAAGC CTGAATGGCT CTGAATTCAC CTATCTGACA 
GGTTTGCAGT ATCTGGATTT CTCTTCAAAT CGCTTGGACC TGCTCTACTC CACCGCCTTC 
CAAGAGCTGA AAAATCTGGT CATCTTAGAT ATTAGTAACA ACAACCATTA TTTTGAATCT 
GAGGGCTTGA CTCACATGCT TAATTTCACC CAAAACCTGA AAAATCTCAA AATACTGATG 
ATGAACCACA ACAAAATCTC CACTTCCACT AACACGGAGC TGGAGAGCCA GTCTCTGGAG 
AGGTTGGAGT TCAGAGGCAA TCGCTTAGAT ATGTTGTGGA GAGATGGCAA CACAAGATAT 
CTTAATTATT TTAAGAAATT ATCCAATCTG GCTGTCCTTG ATATCTCACA TAACAACCTG 
AATTCCATTC AACAGCAAGT GTTTAGCGGC TTGCCAGACA CACTCTCTGA GCTCTATGTG 
AATAACAACA AACTTAAATC CTTTGCTTGG GGAGAACTTT CGCTCTTGCC TTCTCTGCTC 
GTCTTAGATC TCAGTGGAAA CTACTTAACT ACAGTTCCAC ACATGCTGTC AAACTGCTCC 
AAATCTCTCA AAAAGCTCAT TTTACATAAG AACGAAATCC TAAAGCTGAC TCCAGATTTC 
CTTAAGTATG CCTACAACTT GAAATATCTG GATCTGAGTT TTAATCGCAT AGGGCAAATT 
GAGAAATCAA ATTTCCCAGA TGACGTTGTA AATGGGATGG ACATGCTGCT TCTTCATAAA 
AACAGATTCC AGTGCAGCTG CAGTGCTATT TGGTTTGTCA TGTGGCTCAA CCAGACTACA 
GTGACCATCC CCAGACTCGC TACAGATGTG ACCTGTGCTT CTCCAGGGGC ACAGAGGGGT 
CATCCCGTGA TCTCAGTGGA TCTGTTGGCC TGTCAGCACA ACTTCCTGTC AATCATTCTC 
TACTTTCTCA TGACTTCCTT CACCCTGATT TTCTTCACAC TGGCCATCTC CAGTCACCTC 
TTCCTGTGGG ACGTCTGGTA CATCTACCAC TTCTGCAGGG CCAAGCTCAA GGGCTACAGT 
CAACTCTCCT CTCAAAGCTC TGTCTACGAT GCATTTGTGA TGTATGACAA AGAGGATTCT 
GCGGTGTCGG AGTGGGTGAT GAAGGAAATG TGCATGCAGC TGGAGGGTCG AGAAGACCGA 
CGTCTGACGC TGTGTCTGGA GGAACGGGAC TGGATACCCG GCACTCCCGT CATCGATAAC 
CTTTCCCAGA GCATCCACAG AAGCAAGAGG ACTGTATTCA TTCTTACAAA CACATACATC 
AAAAGCGGCA GCTTCAAAAC CGCCTTCTAC ATGGCCCACC AAAGGCTAAT GGATGAAAAG 
AACGATGTTA TTGTTCTGAT CTTCCTGGAG AACATACCTT ATAATTCCAG GTATCTGAGA 
CTGAGGAGAA GACTGTATAG ACGGTCTGTG CTGGAGTGGC CGACGAACCC TCAGGCCCGT 
CCATATTTCT GGTTCAGCGT GAGAAGTGTG TTGGCAACAG AAAGCCACAA ACAATACAAC 
AACCTCTTTA AAGAGACACT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101074686 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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