LOC101073726 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101073726
Entrez Gene ID	101073726
Full Name	centromere protein T
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011602693.2	XP_011600995.2	centromere protein T isoform X1
XM_011602693.1	XP_011600995.1	centromere protein T isoform X1
XM_011602699.1	XP_011601001.1	centromere protein T isoform X2
XM_011602699.2	XP_011601001.2	centromere protein T isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC101073726 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101073726 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo27777	XM_011602693.1 Latest version!	Takifugu rubripes centromere protein T (cenpt), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OTo27778	XM_011602699.1 Latest version!	Takifugu rubripes centromere protein T (cenpt), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OTo41626	XM_011602693.2 Latest version!	Takifugu rubripes centromere protein T (LOC101073726), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OTo41627	XM_011602699.2 Latest version!	Takifugu rubripes centromere protein T (LOC101073726), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101073726 ( XM_011602693.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011600995.1 Takifugu rubripes centromere protein T (cenpt), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo27777
Clone ID Related Accession (Same CDS sequence)	XM_011602693.1
Accession Version	XM_011602693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	centromere protein T isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070901.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTA CGGAGGACTT ATCTGCTCGG GTCCTGCTAA GACATATTCT TAGCACCGAG 
CCGCCTAGAA CTCCAATCAC CCGCAGTGTG ACCAAAGCAC AGAGTCTCAG CGTGACCAGG 
CGCAGCAGCC GACTGAGTAA TAAGGACAAT GTTGGTCAAA CACCACAGGA CTTACTGAGG 
CGAAACCTGC GACAGAAAAT GCGCGAGAGT ATAACAACAA CGTCATTGCC CCCCACCAAG 
AGAAGGACCG CCGTCAAGAG AGAGTCCAAC ACAGCAGCCC TAATGTCTTT TGATGATGGA 
GGCACACCGA GACACATCCT GAAGAACATC CTGATGACAG AGCCTGTAAG GTCCCCGGTG 
GTTCATAGGA AGACAGAGGC AGAAGAGCCA CAGCCATTTT CAGCAAATTC CAGCCTCACC 
GGCAAGAACA CGAGCATTGA ATTATCAGGA CTGGACTTGC CTGATCTTAC CATGGGCATT 
GCTCCAACGG TTACTAAGGG ACTGAGCAGA AAGAGACAAC GGCAGAGTCT AAACGTCACC 
GCTTTTGAGA AGCGTCTTAA AGGTGGAGAT GATGTTGGAG AGGAAAGTGA GCTATGGGCA 
AATGAACATT CATCACTCTC TTTATCAGGA TCTACATCTT TGAGTCTGAA GACTCCTTTT 
GCAGATGTCC AGACTGAAAA AAGAGGTCTG CAGAGAAGAG TCTCTAACCG CAGGAAAATC 
ACAGAAGAAG CCTTTGGTGC TGCTGTAAAT AGAATAGAGA TGGGAGGGAT GAGCAGCTTT 
GTTGGACTGG GTCCCAGTGA CAACACCTGC ACTGAAGGCT TAACTTTGGG CTTAAGCACA 
TTCAGCGAAC CTGATATCAC AACAGATATT GTTCAGTGTG ACACTGATCG CGATACCCGA 
TTGAAAGAGG AATCTACAAC CAGGTCTCAC TATAAAGAGG AGGAGAGTGC ACGAGACTCT 
CAAACTGAAG AAAGTGATGG TGATCCTCAG CCTGAAGTAG ATGTTTCCAC TGGGACACAA 
TCTCAAGAAG AGGAGGAGGA GGAGGAGGGT GCCCAAAAGT CTCACAGTGA GGAAGAATGT 
GGTGATCCTC AGCCTGAAGT AGATGTTTCC ACCGGGACAC AATCTCAAGA AGACGAGGAG 
GAGGAGGAGG GTGCCCAAAA GTCTCACAGT GAGGAAGAAT GTGGTGATCC TGAGCCTGAA 
GTCGAAGTTT CTACTGGGTC ACAGTCTCAA GAAGAGGAGG AGGAAGAGGA GGGTGCCCAA 
GAGTCTCACA AGGAGGAAGA ATGTGGTGAT CCTGAGCCTG AAGTCGAAGT TTCTACCGCG 
TCACAATCTC AAGAAGAGGA GGAGGAGGAT GAAGAGGAGG AGGAGGGTGC CCAAAAGTCT 
CACAGTGAGG AAGAATGTGG TGATCCTGAG CCTGAAGTCG AAGTTTCTAC TGGGTCACAA 
TCTCAAGAAG AGGAGGAGGA GGAGGAGGGT GCCCAAGAGT CTCACACTGC TAATTCTCAA 
ACTAAAGACT TAAATCCTGT TGATTGTCAG GCTGACGAAG AGGAGGAGGA TTTTGCAGAT 
GATGAGAGAG GGCACAAAAA TTCACCCGAG GAGGATTTGA CTCATTCTGA AGCTGAGGAG 
CTAGAGGAGC ATGGTGACGG GCGCGTATCA GAAGAGCACG AGGCTCATTG TTCTGGAGGT 
GTTGCCGTGC ACGCCACAGA AACAGACGGA GACGCCTCAG ACACAGGATG CTCTGAGGAC 
GAGTCCAAAG CCAAGGGGAC ATTTATTCTA CCCATTACTC TGGGAATGGG AGACAGTGAG 
GCTGACCAGC CTCTCAACGA TTTCGCTGAG TCGTCAACCA CTGGACGGCC AGATGTGGAG 
TATCCTGAGG ATCTGGTCGA TAAAGAGAAC TGTTCGGAAG ACGGCCGACG CCATGATTCC 
CCTGCAGAAG GTGCTGCAGA CGATAGTGAT GAGTCGGAAG ATGAGGAAGA GAGGGAAGAC 
GTCCCCAGCA AGACTCCAGC CTTTGTCAGA AAGAAAAGAG TGTTTTCCCA ACCTTTTGAT 
CACAATGTTC AGCCCAGCTT TGGTTTTTGT TCCAGTGGTC CCAGTAAAAG CTCCCCTGCT 
AAATCAAAGC AGGTCAGAAA GCCGGCACGG CCCAGGAAAA GGCAGGAAGG CCTTCCAAAG 
TCTTACCTCA TGAATGTTTT CAAGCATTTT GCCAAAACCA AAGTCTCTGC TGATGTGTAT 
CCTGTCCTAA ATGAAGTAAT GGATAAGTTC TTTGAGAGGC TTGCAGAGGA TTTAGAGACA 
TATGCTGCTC ATGCAAAGAG AAAAACCATA GACGTTGAAG ATGCTAAGCT TCTTTTGAAA 
AGGCAAGGTC ATGTGAGTGA CAAGGTGCCA GTGGAGGTGC TTATTGAAAA ATATCTTCGC 
TTGGAACAAC GCAAGCTCCT GATCCCAATT GCGACGAGTG GAAATGTTGT TATACCTAAA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011600995.1
CDS	216..2681
Translation	MDPTEDLSARVLLRHILSTEPPRTPITRSVTKAQSLSVTRRSSRLSNKDNVGQTPQDLLRRNLRQKMRESITTTSLPPTKRRTAVKRESNTAALMSFDDGGTPRHILKNILMTEPVRSPVVHRKTEAEEPQPFSANSSLTGKNTSIELSGLDLPDLTMGIAPTVTKGLSRKRQRQSLNVTAFEKRLKGGDDVGEESELWANEHSSLSLSGSTSLSLKTPFADVQTEKRGLQRRVSNRRKITEEAFGAAVNRIEMGGMSSFVGLGPSDNTCTEGLTLGLSTFSEPDITTDIVQCDTDRDTRLKEESTTRSHYKEEESARDSQTEESDGDPQPEVDVSTGTQSQEEEEEEEGAQKSHSEEECGDPQPEVDVSTGTQSQEDEEEEEGAQKSHSEEECGDPEPEVEVSTGSQSQEEEEEEEGAQESHKEEECGDPEPEVEVSTASQSQEEEEEDEEEEEGAQKSHSEEECGDPEPEVEVSTGSQSQEEEEEEEGAQESHTANSQTKDLNPVDCQADEEEEDFADDERGHKNSPEEDLTHSEAEELEEHGDGRVSEEHEAHCSGGVAVHATETDGDASDTGCSEDESKAKGTFILPITLGMGDSEADQPLNDFAESSTTGRPDVEYPEDLVDKENCSEDGRRHDSPAEGAADDSDESEDEEEREDVPSKTPAFVRKKRVFSQPFDHNVQPSFGFCSSGPSKSSPAKSKQVRKPARPRKRQEGLPKSYLMNVFKHFAKTKVSADVYPVLNEVMDKFFERLAEDLETYAAHAKRKTIDVEDAKLLLKRQGHVSDKVPVEVLIEKYLRLEQRKLLIPIATSGNVVIPKK

Target ORF information:

RefSeq Version	XM_011602693.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes centromere protein T (cenpt), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011602693.1

ORF Insert Sequence:

ATGGATCCTA CGGAGGACTT ATCTGCTCGG GTCCTGCTAA GACATATTCT TAGCACCGAG 
CCGCCTAGAA CTCCAATCAC CCGCAGTGTG ACCAAAGCAC AGAGTCTCAG CGTGACCAGG 
CGCAGCAGCC GACTGAGTAA TAAGGACAAT GTTGGTCAAA CACCACAGGA CTTACTGAGG 
CGAAACCTGC GACAGAAAAT GCGCGAGAGT ATAACAACAA CGTCATTGCC CCCCACCAAG 
AGAAGGACCG CCGTCAAGAG AGAGTCCAAC ACAGCAGCCC TAATGTCTTT TGATGATGGA 
GGCACACCGA GACACATCCT GAAGAACATC CTGATGACAG AGCCTGTAAG GTCCCCGGTG 
GTTCATAGGA AGACAGAGGC AGAAGAGCCA CAGCCATTTT CAGCAAATTC CAGCCTCACC 
GGCAAGAACA CGAGCATTGA ATTATCAGGA CTGGACTTGC CTGATCTTAC CATGGGCATT 
GCTCCAACGG TTACTAAGGG ACTGAGCAGA AAGAGACAAC GGCAGAGTCT AAACGTCACC 
GCTTTTGAGA AGCGTCTTAA AGGTGGAGAT GATGTTGGAG AGGAAAGTGA GCTATGGGCA 
AATGAACATT CATCACTCTC TTTATCAGGA TCTACATCTT TGAGTCTGAA GACTCCTTTT 
GCAGATGTCC AGACTGAAAA AAGAGGTCTG CAGAGAAGAG TCTCTAACCG CAGGAAAATC 
ACAGAAGAAG CCTTTGGTGC TGCTGTAAAT AGAATAGAGA TGGGAGGGAT GAGCAGCTTT 
GTTGGACTGG GTCCCAGTGA CAACACCTGC ACTGAAGGCT TAACTTTGGG CTTAAGCACA 
TTCAGCGAAC CTGATATCAC AACAGATATT GTTCAGTGTG ACACTGATCG CGATACCCGA 
TTGAAAGAGG AATCTACAAC CAGGTCTCAC TATAAAGAGG AGGAGAGTGC ACGAGACTCT 
CAAACTGAAG AAAGTGATGG TGATCCTCAG CCTGAAGTAG ATGTTTCCAC TGGGACACAA 
TCTCAAGAAG AGGAGGAGGA GGAGGAGGGT GCCCAAAAGT CTCACAGTGA GGAAGAATGT 
GGTGATCCTC AGCCTGAAGT AGATGTTTCC ACCGGGACAC AATCTCAAGA AGACGAGGAG 
GAGGAGGAGG GTGCCCAAAA GTCTCACAGT GAGGAAGAAT GTGGTGATCC TGAGCCTGAA 
GTCGAAGTTT CTACTGGGTC ACAGTCTCAA GAAGAGGAGG AGGAAGAGGA GGGTGCCCAA 
GAGTCTCACA AGGAGGAAGA ATGTGGTGAT CCTGAGCCTG AAGTCGAAGT TTCTACCGCG 
TCACAATCTC AAGAAGAGGA GGAGGAGGAT GAAGAGGAGG AGGAGGGTGC CCAAAAGTCT 
CACAGTGAGG AAGAATGTGG TGATCCTGAG CCTGAAGTCG AAGTTTCTAC TGGGTCACAA 
TCTCAAGAAG AGGAGGAGGA GGAGGAGGGT GCCCAAGAGT CTCACACTGC TAATTCTCAA 
ACTAAAGACT TAAATCCTGT TGATTGTCAG GCTGACGAAG AGGAGGAGGA TTTTGCAGAT 
GATGAGAGAG GGCACAAAAA TTCACCCGAG GAGGATTTGA CTCATTCTGA AGCTGAGGAG 
CTAGAGGAGC ATGGTGACGG GCGCGTATCA GAAGAGCACG AGGCTCATTG TTCTGGAGGT 
GTTGCCGTGC ACGCCACAGA AACAGACGGA GACGCCTCAG ACACAGGATG CTCTGAGGAC 
GAGTCCAAAG CCAAGGGGAC ATTTATTCTA CCCATTACTC TGGGAATGGG AGACAGTGAG 
GCTGACCAGC CTCTCAACGA TTTCGCTGAG TCGTCAACCA CTGGACGGCC AGATGTGGAG 
TATCCTGAGG ATCTGGTCGA TAAAGAGAAC TGTTCGGAAG ACGGCCGACG CCATGATTCC 
CCTGCAGAAG GTGCTGCAGA CGATAGTGAT GAGTCGGAAG ATGAGGAAGA GAGGGAAGAC 
GTCCCCAGCA AGACTCCAGC CTTTGTCAGA AAGAAAAGAG TGTTTTCCCA ACCTTTTGAT 
CACAATGTTC AGCCCAGCTT TGGTTTTTGT TCCAGTGGTC CCAGTAAAAG CTCCCCTGCT 
AAATCAAAGC AGGTCAGAAA GCCGGCACGG CCCAGGAAAA GGCAGGAAGG CCTTCCAAAG 
TCTTACCTCA TGAATGTTTT CAAGCATTTT GCCAAAACCA AAGTCTCTGC TGATGTGTAT 
CCTGTCCTAA ATGAAGTAAT GGATAAGTTC TTTGAGAGGC TTGCAGAGGA TTTAGAGACA 
TATGCTGCTC ATGCAAAGAG AAAAACCATA GACGTTGAAG ATGCTAAGCT TCTTTTGAAA 
AGGCAAGGTC ATGTGAGTGA CAAGGTGCCA GTGGAGGTGC TTATTGAAAA ATATCTTCGC 
TTGGAACAAC GCAAGCTCCT GATCCCAATT GCGACGAGTG GAAATGTTGT TATACCTAAA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101073726 ( XM_011602699.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601001.1 Takifugu rubripes centromere protein T (cenpt), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo27778
Clone ID Related Accession (Same CDS sequence)	XM_011602699.1
Accession Version	XM_011602699.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	centromere protein T isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070901.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTA CGGAGGACTT ATCTGCTCGG GTCCTGCTAA GACATATTCT TAGCACCGAG 
CCGCCTAGAA CTCCAATCAC CCGCAGTGTG ACCAAAGCAC AGAGTCTCAG CGTGACCAGG 
CGCAGCAGCC GACTGAGTAA TAAGGACAAT GTTGGTCAAA CACCACAGGA CTTACTGAGG 
CGAAACCTGC GACAGAAAAT GCGCGAGAGT ATAACAACAA CGTCATTGCC CCCCACCAAG 
AGAAGGACCG CCGTCAAGAG AGAGTCCAAC ACAGCAGCCC TAATGTCTTT TGATGATGGA 
GGCACACCGA GACACATCCT GAAGAACATC CTGATGACAG AGCCTGTAAG GTCCCCGGTG 
GTTCATAGGA AGACAGAGGC AGAAGAGCCA CAGCCATTTT CAGCAAATTC CAGCCTCACC 
GGCAAGAACA CGAGCATTGA ATTATCAGGA CTGGACTTGC CTGATCTTAC CATGGGCATT 
GCTCCAACGG TTACTAAGGG ACTGAGCAGA AAGAGACAAC GGCAGAGTCT AAACGTCACC 
GCTTTTGAGA AGCGTCTTAA AGGTGGAGAT GATGTTGGAG AGGAAAGTGA GCTATGGGCA 
AATGAACATT CATCACTCTC TTTATCAGGA TCTACATCTT TGAGTCTGAA GACTCCTTTT 
GCAGATGTCC AGACTGAAAA AAGAGGTCTG CAGAGAAGAG TCTCTAACCG CAGGAAAATC 
ACAGAAGAAG CCTTTGGTGC TGCTGTAAAT AGAATAGAGA TGGGAGGGAT GAGCAGCTTT 
GTTGGACTGG GTCCCAGTGA CAACACCTGC ACTGAAGGCT TAACTTTGGG CTTAAGCACA 
TTCAGCGAAC CTGATATCAC AACAGATATT GTTCAGTGTG ACACTGATCG CGATACCCGA 
TTGAAAGAGG AATCTACAAC CAGGTCTCAC TATAAAGAGG AGGAGAGTGC ACGAGACTCT 
CAAACTGAAG AAAGTGATGG TGATCCTCAG CCTGAAGTAG ATGTTTCCAC TGGGACACAA 
TCTCAAGAAG AGGAGGAGGA GGAGGAGGGT GCCCAAAAGT CTCACAGTGA GGAAGAATGT 
GGTGATCCTC AGCCTGAAGT AGATGTTTCC ACCGGGACAC AATCTCAAGA AGACGAGGAG 
GAGGAGGAGG GTGCCCAAAA GTCTCACAGT GAGGAAGAAT GTGGTGATCC TGAGCCTGAA 
GTCGAAGTTT CTACTGGGTC ACAGTCTCAA GAAGAGGAGG AGGAAGAGGA GGGTGCCCAA 
GAGTCTCACA AGGAGGAAGA ATGTGGTGAT CCTGAGCCTG AAGTCGAAGT TTCTACCGCG 
TCACAATCTC AAGAAGAGGA GGAGGAGGAT GAAGAGGAGG AGGAGGGTGC CCAAAAGTCT 
CACAGTGAGG AAGAATGTGG TGATCCTGAG CCTGAAGTCG AAGTTTCTAC TGGGTCACAA 
TCTCAAGAAG AGGAGGAGGA GGAGGAGGGT GCCCAAGAGT CTCACACTGC TAATTCTCAA 
ACTAAAGACT TAAATCCTGT TGATTGTCAG GCTGACGAAG AGGAGGAGGA TTTTGCAGAT 
GATGAGAGAG GGCACAAAAA TTCACCCGAG GAGGATTTGA CTCATTCTGA AGCTGAGGAG 
CTAGAGGAGC ATGGTGACGG GCGCGTATCA GAAGAGCACG AGGCTCATTG TTCTGGAGGT 
GTTGCCGTGC ACGCCACAGA AACAGACGGA GACGCCTCAG ACACAGGATG CTCTGAGGAC 
GAGTCCAAAG CCAAGGGGAC ATTTATTCTA CCCATTACTC TGGGAATGGG AGACAGTGAG 
GCTGACCAGC CTCTCAACGA TTTCGCTGAG TCGTCAACCA CTGGACGGCC AGATGTGGAG 
TATCCTGAGG ATCTGGTCGA TAAAGAGAAC TGTTCGGAAG ACGGCCGACG CCATGATTCC 
CCTGCAGAAG GTGCTGCAGA CGATAGTGAT GAGTCGGAAG ATGAGGAAGA GAGGGAAGAC 
GTCCCCAGCA AGACTCCAGC CTTTGTCAGA AAGAAAAGAG TGTTTTCCCA ACCTTTTGAT 
CACAATGTTC AGCCCAGTGG TCCCAGTAAA AGCTCCCCTG CTAAATCAAA GCAGGTCAGA 
AAGCCGGCAC GGCCCAGGAA AAGGCAGGAA GGCCTTCCAA AGTCTTACCT CATGAATGTT 
TTCAAGCATT TTGCCAAAAC CAAAGTCTCT GCTGATGTGT ATCCTGTCCT AAATGAAGTA 
ATGGATAAGT TCTTTGAGAG GCTTGCAGAG GATTTAGAGA CATATGCTGC TCATGCAAAG 
AGAAAAACCA TAGACGTTGA AGATGCTAAG CTTCTTTTGA AAAGGCAAGG TCATGTGAGT 
GACAAGGTGC CAGTGGAGGT GCTTATTGAA AAATATCTTC GCTTGGAACA ACGCAAGCTC 
CTGATCCCAA TTGCGACGAG TGGAAATGTT GTTATACCTA AAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601001.1
CDS	216..2663
Translation	MDPTEDLSARVLLRHILSTEPPRTPITRSVTKAQSLSVTRRSSRLSNKDNVGQTPQDLLRRNLRQKMRESITTTSLPPTKRRTAVKRESNTAALMSFDDGGTPRHILKNILMTEPVRSPVVHRKTEAEEPQPFSANSSLTGKNTSIELSGLDLPDLTMGIAPTVTKGLSRKRQRQSLNVTAFEKRLKGGDDVGEESELWANEHSSLSLSGSTSLSLKTPFADVQTEKRGLQRRVSNRRKITEEAFGAAVNRIEMGGMSSFVGLGPSDNTCTEGLTLGLSTFSEPDITTDIVQCDTDRDTRLKEESTTRSHYKEEESARDSQTEESDGDPQPEVDVSTGTQSQEEEEEEEGAQKSHSEEECGDPQPEVDVSTGTQSQEDEEEEEGAQKSHSEEECGDPEPEVEVSTGSQSQEEEEEEEGAQESHKEEECGDPEPEVEVSTASQSQEEEEEDEEEEEGAQKSHSEEECGDPEPEVEVSTGSQSQEEEEEEEGAQESHTANSQTKDLNPVDCQADEEEEDFADDERGHKNSPEEDLTHSEAEELEEHGDGRVSEEHEAHCSGGVAVHATETDGDASDTGCSEDESKAKGTFILPITLGMGDSEADQPLNDFAESSTTGRPDVEYPEDLVDKENCSEDGRRHDSPAEGAADDSDESEDEEEREDVPSKTPAFVRKKRVFSQPFDHNVQPSGPSKSSPAKSKQVRKPARPRKRQEGLPKSYLMNVFKHFAKTKVSADVYPVLNEVMDKFFERLAEDLETYAAHAKRKTIDVEDAKLLLKRQGHVSDKVPVEVLIEKYLRLEQRKLLIPIATSGNVVIPKK

Target ORF information:

RefSeq Version	XM_011602699.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes centromere protein T (cenpt), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011602699.1

ORF Insert Sequence:

ATGGATCCTA CGGAGGACTT ATCTGCTCGG GTCCTGCTAA GACATATTCT TAGCACCGAG 
CCGCCTAGAA CTCCAATCAC CCGCAGTGTG ACCAAAGCAC AGAGTCTCAG CGTGACCAGG 
CGCAGCAGCC GACTGAGTAA TAAGGACAAT GTTGGTCAAA CACCACAGGA CTTACTGAGG 
CGAAACCTGC GACAGAAAAT GCGCGAGAGT ATAACAACAA CGTCATTGCC CCCCACCAAG 
AGAAGGACCG CCGTCAAGAG AGAGTCCAAC ACAGCAGCCC TAATGTCTTT TGATGATGGA 
GGCACACCGA GACACATCCT GAAGAACATC CTGATGACAG AGCCTGTAAG GTCCCCGGTG 
GTTCATAGGA AGACAGAGGC AGAAGAGCCA CAGCCATTTT CAGCAAATTC CAGCCTCACC 
GGCAAGAACA CGAGCATTGA ATTATCAGGA CTGGACTTGC CTGATCTTAC CATGGGCATT 
GCTCCAACGG TTACTAAGGG ACTGAGCAGA AAGAGACAAC GGCAGAGTCT AAACGTCACC 
GCTTTTGAGA AGCGTCTTAA AGGTGGAGAT GATGTTGGAG AGGAAAGTGA GCTATGGGCA 
AATGAACATT CATCACTCTC TTTATCAGGA TCTACATCTT TGAGTCTGAA GACTCCTTTT 
GCAGATGTCC AGACTGAAAA AAGAGGTCTG CAGAGAAGAG TCTCTAACCG CAGGAAAATC 
ACAGAAGAAG CCTTTGGTGC TGCTGTAAAT AGAATAGAGA TGGGAGGGAT GAGCAGCTTT 
GTTGGACTGG GTCCCAGTGA CAACACCTGC ACTGAAGGCT TAACTTTGGG CTTAAGCACA 
TTCAGCGAAC CTGATATCAC AACAGATATT GTTCAGTGTG ACACTGATCG CGATACCCGA 
TTGAAAGAGG AATCTACAAC CAGGTCTCAC TATAAAGAGG AGGAGAGTGC ACGAGACTCT 
CAAACTGAAG AAAGTGATGG TGATCCTCAG CCTGAAGTAG ATGTTTCCAC TGGGACACAA 
TCTCAAGAAG AGGAGGAGGA GGAGGAGGGT GCCCAAAAGT CTCACAGTGA GGAAGAATGT 
GGTGATCCTC AGCCTGAAGT AGATGTTTCC ACCGGGACAC AATCTCAAGA AGACGAGGAG 
GAGGAGGAGG GTGCCCAAAA GTCTCACAGT GAGGAAGAAT GTGGTGATCC TGAGCCTGAA 
GTCGAAGTTT CTACTGGGTC ACAGTCTCAA GAAGAGGAGG AGGAAGAGGA GGGTGCCCAA 
GAGTCTCACA AGGAGGAAGA ATGTGGTGAT CCTGAGCCTG AAGTCGAAGT TTCTACCGCG 
TCACAATCTC AAGAAGAGGA GGAGGAGGAT GAAGAGGAGG AGGAGGGTGC CCAAAAGTCT 
CACAGTGAGG AAGAATGTGG TGATCCTGAG CCTGAAGTCG AAGTTTCTAC TGGGTCACAA 
TCTCAAGAAG AGGAGGAGGA GGAGGAGGGT GCCCAAGAGT CTCACACTGC TAATTCTCAA 
ACTAAAGACT TAAATCCTGT TGATTGTCAG GCTGACGAAG AGGAGGAGGA TTTTGCAGAT 
GATGAGAGAG GGCACAAAAA TTCACCCGAG GAGGATTTGA CTCATTCTGA AGCTGAGGAG 
CTAGAGGAGC ATGGTGACGG GCGCGTATCA GAAGAGCACG AGGCTCATTG TTCTGGAGGT 
GTTGCCGTGC ACGCCACAGA AACAGACGGA GACGCCTCAG ACACAGGATG CTCTGAGGAC 
GAGTCCAAAG CCAAGGGGAC ATTTATTCTA CCCATTACTC TGGGAATGGG AGACAGTGAG 
GCTGACCAGC CTCTCAACGA TTTCGCTGAG TCGTCAACCA CTGGACGGCC AGATGTGGAG 
TATCCTGAGG ATCTGGTCGA TAAAGAGAAC TGTTCGGAAG ACGGCCGACG CCATGATTCC 
CCTGCAGAAG GTGCTGCAGA CGATAGTGAT GAGTCGGAAG ATGAGGAAGA GAGGGAAGAC 
GTCCCCAGCA AGACTCCAGC CTTTGTCAGA AAGAAAAGAG TGTTTTCCCA ACCTTTTGAT 
CACAATGTTC AGCCCAGTGG TCCCAGTAAA AGCTCCCCTG CTAAATCAAA GCAGGTCAGA 
AAGCCGGCAC GGCCCAGGAA AAGGCAGGAA GGCCTTCCAA AGTCTTACCT CATGAATGTT 
TTCAAGCATT TTGCCAAAAC CAAAGTCTCT GCTGATGTGT ATCCTGTCCT AAATGAAGTA 
ATGGATAAGT TCTTTGAGAG GCTTGCAGAG GATTTAGAGA CATATGCTGC TCATGCAAAG 
AGAAAAACCA TAGACGTTGA AGATGCTAAG CTTCTTTTGA AAAGGCAAGG TCATGTGAGT 
GACAAGGTGC CAGTGGAGGT GCTTATTGAA AAATATCTTC GCTTGGAACA ACGCAAGCTC 
CTGATCCCAA TTGCGACGAG TGGAAATGTT GTTATACCTA AAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101073726 ( XM_011602693.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011600995.2 Takifugu rubripes centromere protein T (LOC101073726), transcript variant X1, mRNA.

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CloneID	OTo41626
Clone ID Related Accession (Same CDS sequence)	XM_011602693.2
Accession Version	XM_011602693.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	centromere protein T isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011602693.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTA CGGAGGACTT ATCTGCTCGG GTCCTGCTAA GACATATTCT TAGCACCGAG 
CCGCCTAGAA CTCCAATCAC CCGCAGTGTG ACCAAAGCAC AGAGTCTCAG CGTGACCAGG 
CGCAGCAGCC GACTGAGTAA TAAGGACAAT GTTGGTCAAA CACCACAGGA CTTACTGAGG 
CGAAACCTGC GACAGAAAAT GCGCGAGAGT ATAACAACAA CGTCATTGCC CCCCACCAAG 
AGAAGGACCG CCGTCAAGAG AGAGTCCAAC ACTGCAGCCC TAATGTCTTT TGATGATGGA 
GGCACACCGA GACACATCCT GAAGAACATC CTGATGACAG AGCCTGTAAG GTCCCCGGTG 
GTTCATAGGA AGACAGAGGC AGAAGAGCCA CAGCCATTTT CAGCAAATTC CAGCCTCACC 
GGCAAGAACA CGAGCATTGA ATTATCAGGA CTGGACTTGC CTGATCTTAC CATGGGCATT 
GCTCCAACGG TTACTAAGGG ACTGAGCAGA AAGAGACAAC GGCAGAGTCT AAACGTCACC 
GCTTTTGAGA AGCGTCTTAA AGGTGGAGAT GATGTTGGAG AGGAAAGTGA GCTATGGGCA 
AATGAACATT CATCACTCTC TTTATCAGGA TCTACATCTT TGAGTCTGAA GACTCCTTTT 
GCAGATGTCC AGACTGAAAA AAGAGGTCTG CAGAGAAGAG TCTCTAACCG CAGGAAAATC 
ACAGAAGAAG CCTTTGGTGC TGCTGTAAAT AGAATAGAGA TGGGAGGGAT GAGCAGCTTT 
GTTGGACTGG GTCCCAGTGA CAACACCTGC ACTGAAGGCT TAACTTTGGG CTTAAGCACA 
CTCAGCGAAC CTGATATCAC AACAGATATT GTTCAGTGTG ACACTGATCG CGATACCCGA 
TTGAAAGAGG AATCTACAAC CAGGTCTCAC TATAAAGAGG AGGAGAGTGC ACGAGACTCT 
CAAACTGAAG AAAGTGATGG TGATCCTCAG CCTGAAGTAG ATGTTTCCAC CGGGACACAA 
TCTCAAGAAG AGGAGGAGGA GGAGGAGGGT GCCCAAAAGT CTCACAGTGA GGAAGAATGT 
GGTGATCCTC AGCCTGAAGT CGAAGTTTTT ACCGGGTCAC AATCTCAAGA GGAGGAGGAG 
GAGGGTGCCC AAACGTCTCA CAGTGAGGAA GAATGTGGTG ATCCTGAGCC TGAAGTCGAA 
GTTTTTACCG GGTCACAATC TCAAGAAGAG GAGGAGGAGG ATGAAGAGGA GGAGGAGGGT 
GCCCAAAAGT CTCACAGTGA GGAAGAATGT GGTGATCCTG AGCCTGAAGT CGAAGTTTCT 
ACTGGGTCAC AATCTCAAGA AGAGGAGGAG GAAGAGGAGG GTGCCCAAGA GTCTCACAAG 
GAGGAAGAAT GTGGTGATCC TGAGCCTGAA GTCGAAGTTT TTACCGGGTC ACAATCTCAA 
GAAGAGGAGG AGGAGGATGA AGAGGAGGAG GAGGGTGCCC AAAAGTCTCA CAGTGAGGAA 
GAATGTGGTG ATCCTGAGCC TGAAGTCGAA GTTTCTACTG GGTCACAGTC TCAAGAAGAG 
GAGGAGGAGG AGGAGGGTGC CCAAGAGTCT CACACTGCTA ATTCTCAAAC TAAAGACTTA 
AATCCTGTTG ATTGTCAGGC TGACGAAGAG GAGGAGGATT TTGCAGATGA TGAGAGAGGG 
CACAAAAATT CACCCGAGGA GGATTTGACT CATTCTGAAG CTGAGGAGCT AGAGGAGCAT 
GGTGACGGGC GCGTATCAGA AGAGCACGAG GCTCATTGTT CTGGAGGTGT TGCCGTGCAC 
GCCACAGAAA CAGACGGAGA CGCCTCAGAC ACAGGATGCT CTGAGGACGA GTCCAAAGCC 
AAGGGGACAT TTATTCTACC CATTACTCTG GGAATGGGAG ACAGTGAGGC TGACCAGCCT 
CTCAACGATT TCGCTGAGTC GTCAACCACT GGACGGCCAG ATGTGGAGTA TCCTGAGGAT 
CTGGTCGATA AAGAGAACTG TTCGGAAGAC GGCCGACGCC ATGATTCCCC TGCAGAAGGT 
GCTGCAGACG ATAGTGATGA GTCGGAAGAT GAGGAAGAGA GGGAAGACGT CCCCAGCAAG 
ACTCCAGCCT TTGTCAGAAA GAAAAGAGTG TTTTCCCAAC CTTTTGATCA CAATGTTCAG 
CCCAGCTTTG GTTTTTGTTC CAGTGGTCCC AGTAAAAGCT CCCCTGCTAA ATCAAAGCAG 
GTCAGAAAGC CGTCACGGCC CAGGAAAAGG CAGGAAGGCC TTCCAAAGTC TTACCTCATG 
AATGTTTTCA AGCATTTTGC CAAAACCAAA GTCTCTGCTG ATGTGTATCC TGTCCTAAAT 
GAAGTAATGG ATAAGTTCTT TGAGAGGCTT GCAGAGGATT TAGAGACATA TGCTGCTCAT 
GCAAAGAGAA AAACCATAGA TGTTGAAGAT GCTAAGCTTC TTTTGAAAAG GCAAGGTCAT 
GTGAGTGACA AGGTGCCAGT GGAGGTGCTT ATTGAAAAAT ATCTTCGCTT GGAACAACGC 
AAGCTCCTGA TCCCAATTGC GACGAGTGGA AATGTTGTTA TAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011600995.2
CDS	226..2793
Translation	MDPTEDLSARVLLRHILSTEPPRTPITRSVTKAQSLSVTRRSSRLSNKDNVGQTPQDLLRRNLRQKMRESITTTSLPPTKRRTAVKRESNTAALMSFDDGGTPRHILKNILMTEPVRSPVVHRKTEAEEPQPFSANSSLTGKNTSIELSGLDLPDLTMGIAPTVTKGLSRKRQRQSLNVTAFEKRLKGGDDVGEESELWANEHSSLSLSGSTSLSLKTPFADVQTEKRGLQRRVSNRRKITEEAFGAAVNRIEMGGMSSFVGLGPSDNTCTEGLTLGLSTLSEPDITTDIVQCDTDRDTRLKEESTTRSHYKEEESARDSQTEESDGDPQPEVDVSTGTQSQEEEEEEEGAQKSHSEEECGDPQPEVEVFTGSQSQEEEEEGAQTSHSEEECGDPEPEVEVFTGSQSQEEEEEDEEEEEGAQKSHSEEECGDPEPEVEVSTGSQSQEEEEEEEGAQESHKEEECGDPEPEVEVFTGSQSQEEEEEDEEEEEGAQKSHSEEECGDPEPEVEVSTGSQSQEEEEEEEGAQESHTANSQTKDLNPVDCQADEEEEDFADDERGHKNSPEEDLTHSEAEELEEHGDGRVSEEHEAHCSGGVAVHATETDGDASDTGCSEDESKAKGTFILPITLGMGDSEADQPLNDFAESSTTGRPDVEYPEDLVDKENCSEDGRRHDSPAEGAADDSDESEDEEEREDVPSKTPAFVRKKRVFSQPFDHNVQPSFGFCSSGPSKSSPAKSKQVRKPSRPRKRQEGLPKSYLMNVFKHFAKTKVSADVYPVLNEVMDKFFERLAEDLETYAAHAKRKTIDVEDAKLLLKRQGHVSDKVPVEVLIEKYLRLEQRKLLIPIATSGNVVIT

Target ORF information:

RefSeq Version	XM_011602693.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes centromere protein T (LOC101073726), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011602693.2

ORF Insert Sequence:

ATGGATCCTA CGGAGGACTT ATCTGCTCGG GTCCTGCTAA GACATATTCT TAGCACCGAG 
CCGCCTAGAA CTCCAATCAC CCGCAGTGTG ACCAAAGCAC AGAGTCTCAG CGTGACCAGG 
CGCAGCAGCC GACTGAGTAA TAAGGACAAT GTTGGTCAAA CACCACAGGA CTTACTGAGG 
CGAAACCTGC GACAGAAAAT GCGCGAGAGT ATAACAACAA CGTCATTGCC CCCCACCAAG 
AGAAGGACCG CCGTCAAGAG AGAGTCCAAC ACTGCAGCCC TAATGTCTTT TGATGATGGA 
GGCACACCGA GACACATCCT GAAGAACATC CTGATGACAG AGCCTGTAAG GTCCCCGGTG 
GTTCATAGGA AGACAGAGGC AGAAGAGCCA CAGCCATTTT CAGCAAATTC CAGCCTCACC 
GGCAAGAACA CGAGCATTGA ATTATCAGGA CTGGACTTGC CTGATCTTAC CATGGGCATT 
GCTCCAACGG TTACTAAGGG ACTGAGCAGA AAGAGACAAC GGCAGAGTCT AAACGTCACC 
GCTTTTGAGA AGCGTCTTAA AGGTGGAGAT GATGTTGGAG AGGAAAGTGA GCTATGGGCA 
AATGAACATT CATCACTCTC TTTATCAGGA TCTACATCTT TGAGTCTGAA GACTCCTTTT 
GCAGATGTCC AGACTGAAAA AAGAGGTCTG CAGAGAAGAG TCTCTAACCG CAGGAAAATC 
ACAGAAGAAG CCTTTGGTGC TGCTGTAAAT AGAATAGAGA TGGGAGGGAT GAGCAGCTTT 
GTTGGACTGG GTCCCAGTGA CAACACCTGC ACTGAAGGCT TAACTTTGGG CTTAAGCACA 
CTCAGCGAAC CTGATATCAC AACAGATATT GTTCAGTGTG ACACTGATCG CGATACCCGA 
TTGAAAGAGG AATCTACAAC CAGGTCTCAC TATAAAGAGG AGGAGAGTGC ACGAGACTCT 
CAAACTGAAG AAAGTGATGG TGATCCTCAG CCTGAAGTAG ATGTTTCCAC CGGGACACAA 
TCTCAAGAAG AGGAGGAGGA GGAGGAGGGT GCCCAAAAGT CTCACAGTGA GGAAGAATGT 
GGTGATCCTC AGCCTGAAGT CGAAGTTTTT ACCGGGTCAC AATCTCAAGA GGAGGAGGAG 
GAGGGTGCCC AAACGTCTCA CAGTGAGGAA GAATGTGGTG ATCCTGAGCC TGAAGTCGAA 
GTTTTTACCG GGTCACAATC TCAAGAAGAG GAGGAGGAGG ATGAAGAGGA GGAGGAGGGT 
GCCCAAAAGT CTCACAGTGA GGAAGAATGT GGTGATCCTG AGCCTGAAGT CGAAGTTTCT 
ACTGGGTCAC AATCTCAAGA AGAGGAGGAG GAAGAGGAGG GTGCCCAAGA GTCTCACAAG 
GAGGAAGAAT GTGGTGATCC TGAGCCTGAA GTCGAAGTTT TTACCGGGTC ACAATCTCAA 
GAAGAGGAGG AGGAGGATGA AGAGGAGGAG GAGGGTGCCC AAAAGTCTCA CAGTGAGGAA 
GAATGTGGTG ATCCTGAGCC TGAAGTCGAA GTTTCTACTG GGTCACAGTC TCAAGAAGAG 
GAGGAGGAGG AGGAGGGTGC CCAAGAGTCT CACACTGCTA ATTCTCAAAC TAAAGACTTA 
AATCCTGTTG ATTGTCAGGC TGACGAAGAG GAGGAGGATT TTGCAGATGA TGAGAGAGGG 
CACAAAAATT CACCCGAGGA GGATTTGACT CATTCTGAAG CTGAGGAGCT AGAGGAGCAT 
GGTGACGGGC GCGTATCAGA AGAGCACGAG GCTCATTGTT CTGGAGGTGT TGCCGTGCAC 
GCCACAGAAA CAGACGGAGA CGCCTCAGAC ACAGGATGCT CTGAGGACGA GTCCAAAGCC 
AAGGGGACAT TTATTCTACC CATTACTCTG GGAATGGGAG ACAGTGAGGC TGACCAGCCT 
CTCAACGATT TCGCTGAGTC GTCAACCACT GGACGGCCAG ATGTGGAGTA TCCTGAGGAT 
CTGGTCGATA AAGAGAACTG TTCGGAAGAC GGCCGACGCC ATGATTCCCC TGCAGAAGGT 
GCTGCAGACG ATAGTGATGA GTCGGAAGAT GAGGAAGAGA GGGAAGACGT CCCCAGCAAG 
ACTCCAGCCT TTGTCAGAAA GAAAAGAGTG TTTTCCCAAC CTTTTGATCA CAATGTTCAG 
CCCAGCTTTG GTTTTTGTTC CAGTGGTCCC AGTAAAAGCT CCCCTGCTAA ATCAAAGCAG 
GTCAGAAAGC CGTCACGGCC CAGGAAAAGG CAGGAAGGCC TTCCAAAGTC TTACCTCATG 
AATGTTTTCA AGCATTTTGC CAAAACCAAA GTCTCTGCTG ATGTGTATCC TGTCCTAAAT 
GAAGTAATGG ATAAGTTCTT TGAGAGGCTT GCAGAGGATT TAGAGACATA TGCTGCTCAT 
GCAAAGAGAA AAACCATAGA TGTTGAAGAT GCTAAGCTTC TTTTGAAAAG GCAAGGTCAT 
GTGAGTGACA AGGTGCCAGT GGAGGTGCTT ATTGAAAAAT ATCTTCGCTT GGAACAACGC 
AAGCTCCTGA TCCCAATTGC GACGAGTGGA AATGTTGTTA TAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101073726 ( XM_011602699.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601001.2 Takifugu rubripes centromere protein T (LOC101073726), transcript variant X2, mRNA.

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CloneID	OTo41627
Clone ID Related Accession (Same CDS sequence)	XM_011602699.2
Accession Version	XM_011602699.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	centromere protein T isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011602699.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTA CGGAGGACTT ATCTGCTCGG GTCCTGCTAA GACATATTCT TAGCACCGAG 
CCGCCTAGAA CTCCAATCAC CCGCAGTGTG ACCAAAGCAC AGAGTCTCAG CGTGACCAGG 
CGCAGCAGCC GACTGAGTAA TAAGGACAAT GTTGGTCAAA CACCACAGGA CTTACTGAGG 
CGAAACCTGC GACAGAAAAT GCGCGAGAGT ATAACAACAA CGTCATTGCC CCCCACCAAG 
AGAAGGACCG CCGTCAAGAG AGAGTCCAAC ACTGCAGCCC TAATGTCTTT TGATGATGGA 
GGCACACCGA GACACATCCT GAAGAACATC CTGATGACAG AGCCTGTAAG GTCCCCGGTG 
GTTCATAGGA AGACAGAGGC AGAAGAGCCA CAGCCATTTT CAGCAAATTC CAGCCTCACC 
GGCAAGAACA CGAGCATTGA ATTATCAGGA CTGGACTTGC CTGATCTTAC CATGGGCATT 
GCTCCAACGG TTACTAAGGG ACTGAGCAGA AAGAGACAAC GGCAGAGTCT AAACGTCACC 
GCTTTTGAGA AGCGTCTTAA AGGTGGAGAT GATGTTGGAG AGGAAAGTGA GCTATGGGCA 
AATGAACATT CATCACTCTC TTTATCAGGA TCTACATCTT TGAGTCTGAA GACTCCTTTT 
GCAGATGTCC AGACTGAAAA AAGAGGTCTG CAGAGAAGAG TCTCTAACCG CAGGAAAATC 
ACAGAAGAAG CCTTTGGTGC TGCTGTAAAT AGAATAGAGA TGGGAGGGAT GAGCAGCTTT 
GTTGGACTGG GTCCCAGTGA CAACACCTGC ACTGAAGGCT TAACTTTGGG CTTAAGCACA 
CTCAGCGAAC CTGATATCAC AACAGATATT GTTCAGTGTG ACACTGATCG CGATACCCGA 
TTGAAAGAGG AATCTACAAC CAGGTCTCAC TATAAAGAGG AGGAGAGTGC ACGAGACTCT 
CAAACTGAAG AAAGTGATGG TGATCCTCAG CCTGAAGTAG ATGTTTCCAC CGGGACACAA 
TCTCAAGAAG AGGAGGAGGA GGAGGAGGGT GCCCAAAAGT CTCACAGTGA GGAAGAATGT 
GGTGATCCTC AGCCTGAAGT CGAAGTTTTT ACCGGGTCAC AATCTCAAGA GGAGGAGGAG 
GAGGGTGCCC AAACGTCTCA CAGTGAGGAA GAATGTGGTG ATCCTGAGCC TGAAGTCGAA 
GTTTTTACCG GGTCACAATC TCAAGAAGAG GAGGAGGAGG ATGAAGAGGA GGAGGAGGGT 
GCCCAAAAGT CTCACAGTGA GGAAGAATGT GGTGATCCTG AGCCTGAAGT CGAAGTTTCT 
ACTGGGTCAC AATCTCAAGA AGAGGAGGAG GAAGAGGAGG GTGCCCAAGA GTCTCACAAG 
GAGGAAGAAT GTGGTGATCC TGAGCCTGAA GTCGAAGTTT TTACCGGGTC ACAATCTCAA 
GAAGAGGAGG AGGAGGATGA AGAGGAGGAG GAGGGTGCCC AAAAGTCTCA CAGTGAGGAA 
GAATGTGGTG ATCCTGAGCC TGAAGTCGAA GTTTCTACTG GGTCACAGTC TCAAGAAGAG 
GAGGAGGAGG AGGAGGGTGC CCAAGAGTCT CACACTGCTA ATTCTCAAAC TAAAGACTTA 
AATCCTGTTG ATTGTCAGGC TGACGAAGAG GAGGAGGATT TTGCAGATGA TGAGAGAGGG 
CACAAAAATT CACCCGAGGA GGATTTGACT CATTCTGAAG CTGAGGAGCT AGAGGAGCAT 
GGTGACGGGC GCGTATCAGA AGAGCACGAG GCTCATTGTT CTGGAGGTGT TGCCGTGCAC 
GCCACAGAAA CAGACGGAGA CGCCTCAGAC ACAGGATGCT CTGAGGACGA GTCCAAAGCC 
AAGGGGACAT TTATTCTACC CATTACTCTG GGAATGGGAG ACAGTGAGGC TGACCAGCCT 
CTCAACGATT TCGCTGAGTC GTCAACCACT GGACGGCCAG ATGTGGAGTA TCCTGAGGAT 
CTGGTCGATA AAGAGAACTG TTCGGAAGAC GGCCGACGCC ATGATTCCCC TGCAGAAGGT 
GCTGCAGACG ATAGTGATGA GTCGGAAGAT GAGGAAGAGA GGGAAGACGT CCCCAGCAAG 
ACTCCAGCCT TTGTCAGAAA GAAAAGAGTG TTTTCCCAAC CTTTTGATCA CAATGTTCAG 
CCCAGTGGTC CCAGTAAAAG CTCCCCTGCT AAATCAAAGC AGGTCAGAAA GCCGTCACGG 
CCCAGGAAAA GGCAGGAAGG CCTTCCAAAG TCTTACCTCA TGAATGTTTT CAAGCATTTT 
GCCAAAACCA AAGTCTCTGC TGATGTGTAT CCTGTCCTAA ATGAAGTAAT GGATAAGTTC 
TTTGAGAGGC TTGCAGAGGA TTTAGAGACA TATGCTGCTC ATGCAAAGAG AAAAACCATA 
GATGTTGAAG ATGCTAAGCT TCTTTTGAAA AGGCAAGGTC ATGTGAGTGA CAAGGTGCCA 
GTGGAGGTGC TTATTGAAAA ATATCTTCGC TTGGAACAAC GCAAGCTCCT GATCCCAATT 
GCGACGAGTG GAAATGTTGT TATAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601001.2
CDS	225..2774
Translation	MDPTEDLSARVLLRHILSTEPPRTPITRSVTKAQSLSVTRRSSRLSNKDNVGQTPQDLLRRNLRQKMRESITTTSLPPTKRRTAVKRESNTAALMSFDDGGTPRHILKNILMTEPVRSPVVHRKTEAEEPQPFSANSSLTGKNTSIELSGLDLPDLTMGIAPTVTKGLSRKRQRQSLNVTAFEKRLKGGDDVGEESELWANEHSSLSLSGSTSLSLKTPFADVQTEKRGLQRRVSNRRKITEEAFGAAVNRIEMGGMSSFVGLGPSDNTCTEGLTLGLSTLSEPDITTDIVQCDTDRDTRLKEESTTRSHYKEEESARDSQTEESDGDPQPEVDVSTGTQSQEEEEEEEGAQKSHSEEECGDPQPEVEVFTGSQSQEEEEEGAQTSHSEEECGDPEPEVEVFTGSQSQEEEEEDEEEEEGAQKSHSEEECGDPEPEVEVSTGSQSQEEEEEEEGAQESHKEEECGDPEPEVEVFTGSQSQEEEEEDEEEEEGAQKSHSEEECGDPEPEVEVSTGSQSQEEEEEEEGAQESHTANSQTKDLNPVDCQADEEEEDFADDERGHKNSPEEDLTHSEAEELEEHGDGRVSEEHEAHCSGGVAVHATETDGDASDTGCSEDESKAKGTFILPITLGMGDSEADQPLNDFAESSTTGRPDVEYPEDLVDKENCSEDGRRHDSPAEGAADDSDESEDEEEREDVPSKTPAFVRKKRVFSQPFDHNVQPSGPSKSSPAKSKQVRKPSRPRKRQEGLPKSYLMNVFKHFAKTKVSADVYPVLNEVMDKFFERLAEDLETYAAHAKRKTIDVEDAKLLLKRQGHVSDKVPVEVLIEKYLRLEQRKLLIPIATSGNVVIT

Target ORF information:

RefSeq Version	XM_011602699.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes centromere protein T (LOC101073726), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011602699.2

ORF Insert Sequence:

ATGGATCCTA CGGAGGACTT ATCTGCTCGG GTCCTGCTAA GACATATTCT TAGCACCGAG 
CCGCCTAGAA CTCCAATCAC CCGCAGTGTG ACCAAAGCAC AGAGTCTCAG CGTGACCAGG 
CGCAGCAGCC GACTGAGTAA TAAGGACAAT GTTGGTCAAA CACCACAGGA CTTACTGAGG 
CGAAACCTGC GACAGAAAAT GCGCGAGAGT ATAACAACAA CGTCATTGCC CCCCACCAAG 
AGAAGGACCG CCGTCAAGAG AGAGTCCAAC ACTGCAGCCC TAATGTCTTT TGATGATGGA 
GGCACACCGA GACACATCCT GAAGAACATC CTGATGACAG AGCCTGTAAG GTCCCCGGTG 
GTTCATAGGA AGACAGAGGC AGAAGAGCCA CAGCCATTTT CAGCAAATTC CAGCCTCACC 
GGCAAGAACA CGAGCATTGA ATTATCAGGA CTGGACTTGC CTGATCTTAC CATGGGCATT 
GCTCCAACGG TTACTAAGGG ACTGAGCAGA AAGAGACAAC GGCAGAGTCT AAACGTCACC 
GCTTTTGAGA AGCGTCTTAA AGGTGGAGAT GATGTTGGAG AGGAAAGTGA GCTATGGGCA 
AATGAACATT CATCACTCTC TTTATCAGGA TCTACATCTT TGAGTCTGAA GACTCCTTTT 
GCAGATGTCC AGACTGAAAA AAGAGGTCTG CAGAGAAGAG TCTCTAACCG CAGGAAAATC 
ACAGAAGAAG CCTTTGGTGC TGCTGTAAAT AGAATAGAGA TGGGAGGGAT GAGCAGCTTT 
GTTGGACTGG GTCCCAGTGA CAACACCTGC ACTGAAGGCT TAACTTTGGG CTTAAGCACA 
CTCAGCGAAC CTGATATCAC AACAGATATT GTTCAGTGTG ACACTGATCG CGATACCCGA 
TTGAAAGAGG AATCTACAAC CAGGTCTCAC TATAAAGAGG AGGAGAGTGC ACGAGACTCT 
CAAACTGAAG AAAGTGATGG TGATCCTCAG CCTGAAGTAG ATGTTTCCAC CGGGACACAA 
TCTCAAGAAG AGGAGGAGGA GGAGGAGGGT GCCCAAAAGT CTCACAGTGA GGAAGAATGT 
GGTGATCCTC AGCCTGAAGT CGAAGTTTTT ACCGGGTCAC AATCTCAAGA GGAGGAGGAG 
GAGGGTGCCC AAACGTCTCA CAGTGAGGAA GAATGTGGTG ATCCTGAGCC TGAAGTCGAA 
GTTTTTACCG GGTCACAATC TCAAGAAGAG GAGGAGGAGG ATGAAGAGGA GGAGGAGGGT 
GCCCAAAAGT CTCACAGTGA GGAAGAATGT GGTGATCCTG AGCCTGAAGT CGAAGTTTCT 
ACTGGGTCAC AATCTCAAGA AGAGGAGGAG GAAGAGGAGG GTGCCCAAGA GTCTCACAAG 
GAGGAAGAAT GTGGTGATCC TGAGCCTGAA GTCGAAGTTT TTACCGGGTC ACAATCTCAA 
GAAGAGGAGG AGGAGGATGA AGAGGAGGAG GAGGGTGCCC AAAAGTCTCA CAGTGAGGAA 
GAATGTGGTG ATCCTGAGCC TGAAGTCGAA GTTTCTACTG GGTCACAGTC TCAAGAAGAG 
GAGGAGGAGG AGGAGGGTGC CCAAGAGTCT CACACTGCTA ATTCTCAAAC TAAAGACTTA 
AATCCTGTTG ATTGTCAGGC TGACGAAGAG GAGGAGGATT TTGCAGATGA TGAGAGAGGG 
CACAAAAATT CACCCGAGGA GGATTTGACT CATTCTGAAG CTGAGGAGCT AGAGGAGCAT 
GGTGACGGGC GCGTATCAGA AGAGCACGAG GCTCATTGTT CTGGAGGTGT TGCCGTGCAC 
GCCACAGAAA CAGACGGAGA CGCCTCAGAC ACAGGATGCT CTGAGGACGA GTCCAAAGCC 
AAGGGGACAT TTATTCTACC CATTACTCTG GGAATGGGAG ACAGTGAGGC TGACCAGCCT 
CTCAACGATT TCGCTGAGTC GTCAACCACT GGACGGCCAG ATGTGGAGTA TCCTGAGGAT 
CTGGTCGATA AAGAGAACTG TTCGGAAGAC GGCCGACGCC ATGATTCCCC TGCAGAAGGT 
GCTGCAGACG ATAGTGATGA GTCGGAAGAT GAGGAAGAGA GGGAAGACGT CCCCAGCAAG 
ACTCCAGCCT TTGTCAGAAA GAAAAGAGTG TTTTCCCAAC CTTTTGATCA CAATGTTCAG 
CCCAGTGGTC CCAGTAAAAG CTCCCCTGCT AAATCAAAGC AGGTCAGAAA GCCGTCACGG 
CCCAGGAAAA GGCAGGAAGG CCTTCCAAAG TCTTACCTCA TGAATGTTTT CAAGCATTTT 
GCCAAAACCA AAGTCTCTGC TGATGTGTAT CCTGTCCTAA ATGAAGTAAT GGATAAGTTC 
TTTGAGAGGC TTGCAGAGGA TTTAGAGACA TATGCTGCTC ATGCAAAGAG AAAAACCATA 
GATGTTGAAG ATGCTAAGCT TCTTTTGAAA AGGCAAGGTC ATGTGAGTGA CAAGGTGCCA 
GTGGAGGTGC TTATTGAAAA ATATCTTCGC TTGGAACAAC GCAAGCTCCT GATCCCAATT 
GCGACGAGTG GAAATGTTGT TATAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101073726 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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