LOC101071578 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101071578
Entrez Gene ID	101071578
Full Name	triple functional domain protein-like
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011605456.1	XP_011603758.1	triple functional domain protein-like
XM_029839219.1	XP_029695079.1	triple functional domain protein-like isoform X3
XM_029839218.1	XP_029695078.1	triple functional domain protein-like isoform X2
XM_029839220.1	XP_029695080.1	triple functional domain protein-like isoform X4
XM_029839217.1	XP_029695077.1	triple functional domain protein-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC101071578 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101071578 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo26252	XM_011605456.1 Latest version!	Takifugu rubripes triple functional domain protein-like (LOC101071578), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OTo69928	XM_029839218.1 Latest version!	Takifugu rubripes triple functional domain protein-like (LOC101071578), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OTo69927	XM_029839217.1 Latest version!	Takifugu rubripes triple functional domain protein-like (LOC101071578), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OTo69929	XM_029839219.1 Latest version!	Takifugu rubripes triple functional domain protein-like (LOC101071578), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OTo69930	XM_029839220.1 Latest version!	Takifugu rubripes triple functional domain protein-like (LOC101071578), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101071578 ( XM_011605456.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011603758.1 Takifugu rubripes triple functional domain protein-like (LOC101071578), mRNA.

Price & Availability↑

CloneID	OTo26252
Clone ID Related Accession (Same CDS sequence)	XM_011605456.1
Accession Version	XM_011605456.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	triple functional domain protein-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070985.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGAAATAT CTGCACCTAA AACCCTCCAA TGTTCCCTCA ATATTTGTCA AAATGAGCCC 
ATCAATAGGG AGTTGCAGAA GGAATTGCTA GATGACGTAT ACGCAGAATC CGTGGAGGCT 
GTTCAGGACC TGATCAAACG CTTCGGCCAG CAGCAGCAGA CCACCCTGCA GGCTACTGTC 
AATGTCATCA AAGAGGGAGA GGACCTTATC CAACAGCTCA GAGACTCAGC TATCTCAAGC 
AACAAAACTC CCCATAACAG CTCCATGGCC CACATCGAGA GTGTGCTGCA GCAGCTGGAC 
GAGGCTCAGG GCCAGATGGA GGAACTGTTT CAGGAAAGGA AGATCAAACT GGAGCTCTTT 
CTGCAGCTGC GCATCTTCGA GAGGGACGCC ATCGATATCA TCTCTGACCT GGAGTCATGG 
AACGAGGAGC TCTCCCAGCA GATGAGTGAC TTCGACACCG AGGATCTGAC GCTGGCGGAG 
CAGAGGCTGC AGCACCACGC AGACAAGGCG CTCACCATGA ACAACCTGAC CTACGACGTC 
ATCCACCAGG GCCAAGAGCT GCTGCAGTAC GTGACCGAGG TCCAGGCGTC TGGTGTGGAG 
CTGCTGTGCG ACAGAGATGT GGACATGGCC ACACGGGTCC AGGACCTGCT GGAGTTCCTC 
CACGAGAAGC AGCAGGAGCT GGACCTGGCG GCGGAGCAGC ACAGGAGACA CTTGGAACAG 
TGTGTCCAAC TGAGACACCT CCAGGCAGAA GTCAAACAGG TGCTGGGATG GATCCGGAAT 
GGCGAGTCCA TGCTGAACGC TGGTCTGATC ACAGCCAGCT CGTTACAGGA AGCTGAACAG 
CTGCAGAAGG AACATGAACA ATTTCAGCAT GCAATAGAGA AAACTCATCA GAGCGCGCTG 
CAGGTGCAGC AGAAGGCCGA GACCTTACTC CAGGCAAACC ACTATGACAT GGACATGATC 
AGGGACTGTG CAGAAAAGGT GGCAGACCAC TGGCAGCAGC TCATGCTGAA AATGGAAGAC 
CGACTCAAGC TGGTCAACGC TTCTGTGGCC TTCTACAAAA CCTCCGAACA GGTTTGCAGT 
GTGTTGGAAA GCCTGGAGCA GGAGTACAAG AGAGAAGAGG ACTGGTGTGG TGGTGCTGAT 
AAACTAGGCC CCAACAGCGA GTCCGACCAC GTCACTCCCA TGATCAGCAA ACATCTGGAG 
CAGAAAGAGG CCTTTCTCAA GGCGTGCACG CTAGCCCGGC GGAATGCTGA CGTCTTCTTG 
AAGTACCTGC ACAGAAACAG TGTCAATATG CCCGGCATGC TGTCCCACGT CAAAGCTCCA 
GAGACTCAGG TTAAGAATAT CTTGAACGAG CTGCTCCAGA GAGAGAACCG AGTCCTTCAC 
TTCTGGACAA TGAGGAAGAG GAGGCTGGAC CAGTGTCAGC AATATGTCGT ATTTGAACGC 
AGCGCCAAAC AGGCTCTGGA GTGGATCCAT GACACTGGGG AGTTTTACTT GTCCACACAC 
ACATCCACTG GCTCCAGCAT TCATCACACA CAAGAGCTGC TGAAGGAGCA TGAGGACTTC 
CAGATCACAG CAAAGCAAAC CAAAGAGCGA GTAAAGCTGT TGATCCAGCT GGCAGATGGG 
TTTTGTGACA AGGGGCACGC CCATGCACTG GAAATAAAGA AATGGGTGTC TTCTGTGGAC 
AAGCGCTACC GGGACTTCTC TCTCCGCATG GACAAATACC GTACCTGCCT GGAAACTGCG 
CTCGGCATAT CATCCGACTC CAACAAGGCT AGCAAAGAGC TCCAGCTGGA TATAATCCCA 
GCCAGCGCTC CGGGCTCAGA GGTCAAACTC CGGGATGCTG CCCATGACCT CAATGAGGAG 
AAGAGGAAGT CGGCACGGAG GAAGGAATTC ATAATGGCGG AGCTGATTCA AACAGAAAAA 
GCCTACGTCA GGGACCTAAG GGAGTGTTTG GATACGTACC TGTGGGAGAT GACCAGTGGT 
GTGGAGGAGA TTCCCGCCGG AATTGTCAAC AAAGAGCACA TCATCTTTGG GAACATGCAG 
GACCTCTACG AGTTCCACCA CAACATCTTC CTGAAGGAGT TGGAGAAGTA TGAGCAACTT 
CCAGAAGATG TCGGCCATTG TTTTGTCACT TGGGCTGATA AGTTCCAGAT GTACGTCAGT 
TACTGTAAGA ACAAACCCGA CTCCACGCAG CTCATCCTCG AACATGCCGG AGCATACTTC 
GATGAAATCC AGCAAAGACA TCGCCTTGCT AACTCCATCT CCTCTTACCT GATCAAACCA 
GTGCAGAGAA TCACAAAGTA CCAGCTGCTT CTCAAGGAGC TGCTGACATG CTGCGAGGAG 
GGAAAAGGTG AGATCAAGGA CGGACTGGAA GTGATGCTCA GCGTCCCTAA GAAAGCCAAC 
GACGCCATGC ACCTGTCTAT GCTGGAGGGG TTTGATGGAA ACATTGACTC CCAGGGAGAG 
CTGATACTTC AGGAATCCTT CCAGGTCTGG GATCCTAAGA CTCTGATCCG AAAGGGTCGG 
GACCGGCACC TCTTCCTGTT TGAGATGTCT CTGGTGTTAA GCAAAGAAGT CAAAGATTCC 
AACGGGCGCA GCAAATACCT GTACAAGAGC AAGCTGTTTA CATCAGAACT TGGAGTTACG 
GAACATGTGG AGGGAGATCC CTGTAAGTTC GCCCTGTGGG TGGGCAGGAC CCCAACGTCT 
GACAACAAGA TCGTCCTCAA GGCATCCAGT ATCGAGAACA AACAGGACTG GATCAAACAC 
ATCCGGGAGG TGATCCAGGA GCGGATTGTC CACCTGCGCG GCGCACTGAA GGAGCCCATC 
CACATCCCCA AAACCACCAC AGCCAAACAC AAAGGCAGAC GGGATGGAGA GGAACTGGAC 
AGCCAGGGGG ATGCCAGCAG CCAACCAGAT ACCATCTCCA TTGCCTCGCG AACATCCCAG 
AACACAATGG ACAGCGATAA GCTCTCCGGT GGCTGTGAGT TGACAGTGGT GATCCACGAC 
TTCGTGGCCA GTAATGGCAG CAGCGGCGAG CTGTCGGTGC GCCGAGGTCA GACTGTGGAA 
GTCCTGGAAC GGCTCCACGA CAAGCCCGAC TGGTGCCTGG TGCGGACCAC GGACCGTTCC 
CCGGCTCAGG AGGGCATCGT TCCCTGCTCC ATGCTCTGCA TCGCTCACTC GAGGTCTTCC 
ATGGAGATGG AAGGGCTCTT TAACCACAAA GACACATTAT CTGTATCCAG TAACGACGCC 
CTCCAACCAG GCTCCAGCCA AACCCTGGGT CCTCACAGCT CCCCCGGCCC CAAACGCCCA 
GGGAACACAC TGAGGAAGTG GCTGACCAGC CCCGTGCGGA GGCTCAGCTC TGGCAAGGCC 
GACGGCCACG TGAAGAAACT GGCCCACAAG CATAAGAAGA ACCGCGAGGG CGCGAGGAAG 
AACATGGATG CCATGCACGG TTCTCAGAAG GACTCTGATG ACAGCGCCGC TACGCCCCAG 
GATGAAACGC TAGAGGAACG TATGCGTAAC GAAGGGCTGA GCAGCGGCAC CCTGTCCAAG 
TCTTCCTCTT CTGGCATGCA GAGCTGTGGC GAGGAGGAAG GAGAGGAGGG AGCTGACGCC 
GTCCCTCTAC CGCCCCCTAT GGCCATCCAG CAGCACAGCT TGCTGCAGCC TGACTCCCAG 
GACGACAAGT CCGGATCTCG TCTGTCCGCA CGTCCAAGCA GCTCAGAGAC GCCCAGCGCG 
GCCGAACTAG TCAGCGCCAT CGAGGAGCTG GTGAAGAGTA AGATGTCCCT GGAGGATCGC 
CCCAGCTCTC TGTCAGTAGA ACAAGGTGAC AGCAGCTCTC CCTCCCTAAA TCCCTCTGAC 
AACTCCCTCC TCTCCTCCTC CTCACCCATC GATGAGATGG ACGAGCGCAA GGCCGACTTC 
CTCAAGAGAA GACATTACGT GCTACTGGAG TTGGTGGAGA CCGAGAGGGA CTATGTCAGA 
GATCTGGGAT CAGTGGTAGA GGGCTACATG TGTAGGATGA AAGAAGAGGG TGTTCCTGAT 
GACATGAGGG GAAAAGACAA GATCGTCTTT GGAAACATCC ATCAGATCTA CGACTGGCAC 
AAAGATTTTT TCCTGGGGGA GCTTGAGAAG TGTTTAGAGG ATCCCGACAG GCTGGCACCT 
TTATTCATTA AACAGGAGCG GAGGCTGCAC ATGTACATCG TGTACTGCCA GAACAAACCC 
AAGTCTGAGC ACATCGTTTC AGAGTACATC GACACTTATT TTGAGGACCT AAAGCAGCGT 
TTGGGCCACA GGCTGCAGAT CACTGATCTA TTAATCAAGC CAGTCCAGCG TATAATGAAG 
TACCAATTAC TGCTAAAAGA TCTTTTGAAA ATGTCCAAGA AGGCTGGCTT GGATATGGCA 
GAGCTTGAGA AAGCTGTGGA GGTGATGTGC GTGGTTCCCA AACGCTGCAA CGACATGATG 
AATGTTGGAC GCCTTCAGGG TTTTGAAGGT AAAATTGTGG CGCAGGGCAG ATTACTGCTC 
CAAGATACCT TCATGGTGTC GGACCAGGAT GGGGGACTTC TGTCCAGGAT GAAGGAGAGA 
AGGGTTTTCT TATTTGAACA GATAGTAATC TTTAGCGAAC CTCTTGACAA AAAGAAGGGC 
TTCTCCACTC CAGGATACCT CTTTAAGAAC AGCATCAAGG TGAGCTGGTT GGGTTTAGAA 
GAACATTCAG AGGACCCTTG CAAGTTCACG CTTACCTCCA GATCATCTAG TGGCAACTTG 
GAGAGATACA CCCTCCATTC AATGAGTCCT GGGGTCAGTC AAGTCTGGGT TCACCAGATT 
AGCCAGATAC TGGAGAACCA GCGCAACTTC CTCAATGCTT TGACATCCCC CATTGAATAC 
CAGAGAAACC ATGTGGGTAG TGGAGTGCCA GGTGGTCTTC CCAGCAGCAG CAGCAGCAGT 
ACTGGAGGCC TACCAGGAGG CAGCAGTTCC ATTGGCTCCT CTAGCATGGG AGTAGGAGGC 
GGCGGTGGCT CTGGGGGACC TGGGAACAGC TCATCCTCCT CACTCTGCGG TCCCCGTTCC 
CGACCGTCCC GCATTCCACA GCCATCGTCA CGACTCCCAC AACCCGTCCA CCACCACCAT 
CCCCCGGGCC CTGATGGGCC TGACAGATCA GCAGGTATGT GGCCGCCCTG CCACCCTCAG 
TCCCACCCAT CTAACTCTTA TTCAGACAGG GCCACAAATG GAGAACCATT AGGCTCAGAG 
TTCCCCAAGA TGAAAAGATT GGAGCTTCAC GGAAATAACA GCAACAACAG CAACAAGTCC 
TCAGACAGTT ATGAAGACAC TCAGAAGCAC CCGGCAGCTG GTATACCGCA GATGGCTGTT 
GCGCCTCTGA ATTTGACCCT AAAAAGCCCC CGAGTCGGGA CAGTTTCTCC TCTGATTTCA 
CCTCAGTCTC CTGCAGGAGG AAAAGAAGCA TTTATCCCTT CAAGTCAAGC TCATAAAGGC 
AACTTGTTCT GGGCTGTGGT CCCTCCTATG CCTTCCTCTG TGTCGAGTCG GCCCAGTTCG 
CTCTCCTACC CCAGCGATAG TGTTGGTGGA GGGGATTCTC TGGGAAGAGG CGGCCACTGT 
ACGCCCTCTC ACCATCGCCA GTCCACTCAC AGTAAGGACA TAGACCGCAT GAGCACCTGC 
TCCTCCACCA GCGAGCAGTC CATACAGTCC ATCCAGAGCA ATGGGAGTGA AAACAGTAGC 
AGCAGCATTG TGTCCACCAT GTTGGTGACC CAGGACTATG TGGCAGTGAA GGAGGATGAG 
ATCAGTGTGG TCCAGGGTGA GGTGGTCCAA ATACTGGCCA GCAACCAACA AAACATGTTC 
CTGGTGTACC GGGCAGCCAA CGAGCACTCT CCTGCTGCTG AGGGCTGGAT CCCTGGCTAT 
GTATTAGGAC ACACCTCATC CTCTGTCACA CCTGAGCTCC CTGAAGGAAC CATCAAAAAA 
TCACTATCAT GGCACACAGC ACTCCGAATT CGCCGTAAAT CTGAAAAAAG GGACAAGGAG 
GGCAGGAAGA TCGAGAACGG GTACCGCAAA TCTCAGGACA GCCTGGCCAA CAAAGTCTCT 
GTTAAGCTTC TGAATCCCAA TTTCATTCCT GACGCACCTC CAGAATTCCT CATCCCTGTC 
ACCGATGTAG CATGTGAGAG CGGCGACACT GTGACACTCA GGTGCAAAAT CTGTAGCCGG 
CCTCGCGCCA CCGTTACCTG GCGAGGACCT GACAACGGCG TTCGGAGCAA CAACGGACGC 
TACAGCACCA CCTACAGTGA GACAGAAGAA GCGACTCTCC GTATTGTGAT GGTGTCCGTA 
GAGGACAGTG GCGTGTACAC GTGTGTCGCC ACAAATGTTG CAGGCTCCGT CACGTCCTCC 
GCCAACCTCA CAGTCTCAGC TTCGCATGAT GGCAACAGTG AGATCTGGAA GAGCAGCTTT 
GAGTTCAACT ACACAGAGAT CACAGAACTA GGAAGGGGCC GTTTCTCAGT GACCAAACGG 
TGCGACCAGA GAGGGAGCAA GCGCACGGTT GCGGCCAAAT ACGTTAGCAA GAAGCTGCTG 
CGGCGAGAGC AGGTGCTGCA GGAGATCAGA CTCCTGCAGA CTCTGGACCA TCCCAACCTG 
GTTAAGCTTC TGGACACCTA TGAAATGGCC AACAGCTATG TTCTCGTGCT AGAAATGGCG 
GACCAGGGAC GTTTCTTGGA TTACGTAGTG AGCTGGGGCA ACCTCACAGA GGAGAAGGTG 
GCACTGTACC TCAGAGAAAT TCTTGAAGCT CTTCACTACC TGCACGGCTG GAGGATAGCG 
CACCTCGACC TCAAACCTGA GAACATTGTG GTGGAACATG CATCCTCGCA GCCGGTGATC 
AAGCTGACAG ACTTTGGTGA TGCCGTGCAA CTGAATCCAG CCTCCTCGTA CATCCATCCT 
CTGCTGGGGA GCCCAGAGTT CTCTGCTCCT GAGCTGGTCC TCGGGCAGCC CTCCTCGCTG 
ACGTCGGACA TCTGGAGTTT AGGGGTGGTG ACATACATTG TACTCAGTGG CGCTTCCCCC 
TTCCTGGATG AGAGTTTGGA GGAGACGTGT CTGAACATCT GTCGCCTGGA CTTCAGCTTC 
CCGGAGGACT ACTTCCAGGG AGTGAGCCTG GCTGCCAAGG ACTTTGTCTG CCTGCTGCTC 
CAGAGCGAGC CGGAGCGGCG GCCCACTGCG TTAGCCTGCC TGCAGGAGCA GTGGCTCCAG 
CCCCGAGGTG TGATCAATAC TGGATTTGTA GGCTCGAAAC ATGCACACGC GTCGCCCAAC 
CATCACACTT TACATCTGGA CACATCCAGA CTCATTTCTT TCATTGAGAG GCGGAAACAC 
CAGAACGACA TTCGGCCCAT AGACACGGTC AGGGCCTTCC TCCACAGCCG CCTGCTTAAC 
AACATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011603758.1
CDS	1..7389
Translation	MEISAPKTLQCSLNICQNEPINRELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQATVNVIKEGEDLIQQLRDSAISSNKTPHNSSMAHIESVLQQLDEAQGQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNEELSQQMSDFDTEDLTLAEQRLQHHADKALTMNNLTYDVIHQGQELLQYVTEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRRHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQKEHEQFQHAIEKTHQSALQVQQKAETLLQANHYDMDMIRDCAEKVADHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSESDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMLSHVKAPETQVKNILNELLQRENRVLHFWTMRKRRLDQCQQYVVFERSAKQALEWIHDTGEFYLSTHTSTGSSIHHTQELLKEHEDFQITAKQTKERVKLLIQLADGFCDKGHAHALEIKKWVSSVDKRYRDFSLRMDKYRTCLETALGISSDSNKASKELQLDIIPASAPGSEVKLRDAAHDLNEEKRKSARRKEFIMAELIQTEKAYVRDLRECLDTYLWEMTSGVEEIPAGIVNKEHIIFGNMQDLYEFHHNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVSYCKNKPDSTQLILEHAGAYFDEIQQRHRLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKKANDAMHLSMLEGFDGNIDSQGELILQESFQVWDPKTLIRKGRDRHLFLFEMSLVLSKEVKDSNGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERIVHLRGALKEPIHIPKTTTAKHKGRRDGEELDSQGDASSQPDTISIASRTSQNTMDSDKLSGGCELTVVIHDFVASNGSSGELSVRRGQTVEVLERLHDKPDWCLVRTTDRSPAQEGIVPCSMLCIAHSRSSMEMEGLFNHKDTLSVSSNDALQPGSSQTLGPHSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKNREGARKNMDAMHGSQKDSDDSAATPQDETLEERMRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKSGSRLSARPSSSETPSAAELVSAIEELVKSKMSLEDRPSSLSVEQGDSSSPSLNPSDNSLLSSSSPIDEMDERKADFLKRRHYVLLELVETERDYVRDLGSVVEGYMCRMKEEGVPDDMRGKDKIVFGNIHQIYDWHKDFFLGELEKCLEDPDRLAPLFIKQERRLHMYIVYCQNKPKSEHIVSEYIDTYFEDLKQRLGHRLQITDLLIKPVQRIMKYQLLLKDLLKMSKKAGLDMAELEKAVEVMCVVPKRCNDMMNVGRLQGFEGKIVAQGRLLLQDTFMVSDQDGGLLSRMKERRVFLFEQIVIFSEPLDKKKGFSTPGYLFKNSIKVSWLGLEEHSEDPCKFTLTSRSSSGNLERYTLHSMSPGVSQVWVHQISQILENQRNFLNALTSPIEYQRNHVGSGVPGGLPSSSSSSTGGLPGGSSSIGSSSMGVGGGGGSGGPGNSSSSSLCGPRSRPSRIPQPSSRLPQPVHHHHPPGPDGPDRSAGMWPPCHPQSHPSNSYSDRATNGEPLGSEFPKMKRLELHGNNSNNSNKSSDSYEDTQKHPAAGIPQMAVAPLNLTLKSPRVGTVSPLISPQSPAGGKEAFIPSSQAHKGNLFWAVVPPMPSSVSSRPSSLSYPSDSVGGGDSLGRGGHCTPSHHRQSTHSKDIDRMSTCSSTSEQSIQSIQSNGSENSSSSIVSTMLVTQDYVAVKEDEISVVQGEVVQILASNQQNMFLVYRAANEHSPAAEGWIPGYVLGHTSSSVTPELPEGTIKKSLSWHTALRIRRKSEKRDKEGRKIENGYRKSQDSLANKVSVKLLNPNFIPDAPPEFLIPVTDVACESGDTVTLRCKICSRPRATVTWRGPDNGVRSNNGRYSTTYSETEEATLRIVMVSVEDSGVYTCVATNVAGSVTSSANLTVSASHDGNSEIWKSSFEFNYTEITELGRGRFSVTKRCDQRGSKRTVAAKYVSKKLLRREQVLQEIRLLQTLDHPNLVKLLDTYEMANSYVLVLEMADQGRFLDYVVSWGNLTEEKVALYLREILEALHYLHGWRIAHLDLKPENIVVEHASSQPVIKLTDFGDAVQLNPASSYIHPLLGSPEFSAPELVLGQPSSLTSDIWSLGVVTYIVLSGASPFLDESLEETCLNICRLDFSFPEDYFQGVSLAAKDFVCLLLQSEPERRPTALACLQEQWLQPRGVINTGFVGSKHAHASPNHHTLHLDTSRLISFIERRKHQNDIRPIDTVRAFLHSRLLNNI

Target ORF information:

RefSeq Version	XM_011605456.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes triple functional domain protein-like (LOC101071578), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011605456.1

ORF Insert Sequence:

ATGGAAATAT CTGCACCTAA AACCCTCCAA TGTTCCCTCA ATATTTGTCA AAATGAGCCC 
ATCAATAGGG AGTTGCAGAA GGAATTGCTA GATGACGTAT ACGCAGAATC CGTGGAGGCT 
GTTCAGGACC TGATCAAACG CTTCGGCCAG CAGCAGCAGA CCACCCTGCA GGCTACTGTC 
AATGTCATCA AAGAGGGAGA GGACCTTATC CAACAGCTCA GAGACTCAGC TATCTCAAGC 
AACAAAACTC CCCATAACAG CTCCATGGCC CACATCGAGA GTGTGCTGCA GCAGCTGGAC 
GAGGCTCAGG GCCAGATGGA GGAACTGTTT CAGGAAAGGA AGATCAAACT GGAGCTCTTT 
CTGCAGCTGC GCATCTTCGA GAGGGACGCC ATCGATATCA TCTCTGACCT GGAGTCATGG 
AACGAGGAGC TCTCCCAGCA GATGAGTGAC TTCGACACCG AGGATCTGAC GCTGGCGGAG 
CAGAGGCTGC AGCACCACGC AGACAAGGCG CTCACCATGA ACAACCTGAC CTACGACGTC 
ATCCACCAGG GCCAAGAGCT GCTGCAGTAC GTGACCGAGG TCCAGGCGTC TGGTGTGGAG 
CTGCTGTGCG ACAGAGATGT GGACATGGCC ACACGGGTCC AGGACCTGCT GGAGTTCCTC 
CACGAGAAGC AGCAGGAGCT GGACCTGGCG GCGGAGCAGC ACAGGAGACA CTTGGAACAG 
TGTGTCCAAC TGAGACACCT CCAGGCAGAA GTCAAACAGG TGCTGGGATG GATCCGGAAT 
GGCGAGTCCA TGCTGAACGC TGGTCTGATC ACAGCCAGCT CGTTACAGGA AGCTGAACAG 
CTGCAGAAGG AACATGAACA ATTTCAGCAT GCAATAGAGA AAACTCATCA GAGCGCGCTG 
CAGGTGCAGC AGAAGGCCGA GACCTTACTC CAGGCAAACC ACTATGACAT GGACATGATC 
AGGGACTGTG CAGAAAAGGT GGCAGACCAC TGGCAGCAGC TCATGCTGAA AATGGAAGAC 
CGACTCAAGC TGGTCAACGC TTCTGTGGCC TTCTACAAAA CCTCCGAACA GGTTTGCAGT 
GTGTTGGAAA GCCTGGAGCA GGAGTACAAG AGAGAAGAGG ACTGGTGTGG TGGTGCTGAT 
AAACTAGGCC CCAACAGCGA GTCCGACCAC GTCACTCCCA TGATCAGCAA ACATCTGGAG 
CAGAAAGAGG CCTTTCTCAA GGCGTGCACG CTAGCCCGGC GGAATGCTGA CGTCTTCTTG 
AAGTACCTGC ACAGAAACAG TGTCAATATG CCCGGCATGC TGTCCCACGT CAAAGCTCCA 
GAGACTCAGG TTAAGAATAT CTTGAACGAG CTGCTCCAGA GAGAGAACCG AGTCCTTCAC 
TTCTGGACAA TGAGGAAGAG GAGGCTGGAC CAGTGTCAGC AATATGTCGT ATTTGAACGC 
AGCGCCAAAC AGGCTCTGGA GTGGATCCAT GACACTGGGG AGTTTTACTT GTCCACACAC 
ACATCCACTG GCTCCAGCAT TCATCACACA CAAGAGCTGC TGAAGGAGCA TGAGGACTTC 
CAGATCACAG CAAAGCAAAC CAAAGAGCGA GTAAAGCTGT TGATCCAGCT GGCAGATGGG 
TTTTGTGACA AGGGGCACGC CCATGCACTG GAAATAAAGA AATGGGTGTC TTCTGTGGAC 
AAGCGCTACC GGGACTTCTC TCTCCGCATG GACAAATACC GTACCTGCCT GGAAACTGCG 
CTCGGCATAT CATCCGACTC CAACAAGGCT AGCAAAGAGC TCCAGCTGGA TATAATCCCA 
GCCAGCGCTC CGGGCTCAGA GGTCAAACTC CGGGATGCTG CCCATGACCT CAATGAGGAG 
AAGAGGAAGT CGGCACGGAG GAAGGAATTC ATAATGGCGG AGCTGATTCA AACAGAAAAA 
GCCTACGTCA GGGACCTAAG GGAGTGTTTG GATACGTACC TGTGGGAGAT GACCAGTGGT 
GTGGAGGAGA TTCCCGCCGG AATTGTCAAC AAAGAGCACA TCATCTTTGG GAACATGCAG 
GACCTCTACG AGTTCCACCA CAACATCTTC CTGAAGGAGT TGGAGAAGTA TGAGCAACTT 
CCAGAAGATG TCGGCCATTG TTTTGTCACT TGGGCTGATA AGTTCCAGAT GTACGTCAGT 
TACTGTAAGA ACAAACCCGA CTCCACGCAG CTCATCCTCG AACATGCCGG AGCATACTTC 
GATGAAATCC AGCAAAGACA TCGCCTTGCT AACTCCATCT CCTCTTACCT GATCAAACCA 
GTGCAGAGAA TCACAAAGTA CCAGCTGCTT CTCAAGGAGC TGCTGACATG CTGCGAGGAG 
GGAAAAGGTG AGATCAAGGA CGGACTGGAA GTGATGCTCA GCGTCCCTAA GAAAGCCAAC 
GACGCCATGC ACCTGTCTAT GCTGGAGGGG TTTGATGGAA ACATTGACTC CCAGGGAGAG 
CTGATACTTC AGGAATCCTT CCAGGTCTGG GATCCTAAGA CTCTGATCCG AAAGGGTCGG 
GACCGGCACC TCTTCCTGTT TGAGATGTCT CTGGTGTTAA GCAAAGAAGT CAAAGATTCC 
AACGGGCGCA GCAAATACCT GTACAAGAGC AAGCTGTTTA CATCAGAACT TGGAGTTACG 
GAACATGTGG AGGGAGATCC CTGTAAGTTC GCCCTGTGGG TGGGCAGGAC CCCAACGTCT 
GACAACAAGA TCGTCCTCAA GGCATCCAGT ATCGAGAACA AACAGGACTG GATCAAACAC 
ATCCGGGAGG TGATCCAGGA GCGGATTGTC CACCTGCGCG GCGCACTGAA GGAGCCCATC 
CACATCCCCA AAACCACCAC AGCCAAACAC AAAGGCAGAC GGGATGGAGA GGAACTGGAC 
AGCCAGGGGG ATGCCAGCAG CCAACCAGAT ACCATCTCCA TTGCCTCGCG AACATCCCAG 
AACACAATGG ACAGCGATAA GCTCTCCGGT GGCTGTGAGT TGACAGTGGT GATCCACGAC 
TTCGTGGCCA GTAATGGCAG CAGCGGCGAG CTGTCGGTGC GCCGAGGTCA GACTGTGGAA 
GTCCTGGAAC GGCTCCACGA CAAGCCCGAC TGGTGCCTGG TGCGGACCAC GGACCGTTCC 
CCGGCTCAGG AGGGCATCGT TCCCTGCTCC ATGCTCTGCA TCGCTCACTC GAGGTCTTCC 
ATGGAGATGG AAGGGCTCTT TAACCACAAA GACACATTAT CTGTATCCAG TAACGACGCC 
CTCCAACCAG GCTCCAGCCA AACCCTGGGT CCTCACAGCT CCCCCGGCCC CAAACGCCCA 
GGGAACACAC TGAGGAAGTG GCTGACCAGC CCCGTGCGGA GGCTCAGCTC TGGCAAGGCC 
GACGGCCACG TGAAGAAACT GGCCCACAAG CATAAGAAGA ACCGCGAGGG CGCGAGGAAG 
AACATGGATG CCATGCACGG TTCTCAGAAG GACTCTGATG ACAGCGCCGC TACGCCCCAG 
GATGAAACGC TAGAGGAACG TATGCGTAAC GAAGGGCTGA GCAGCGGCAC CCTGTCCAAG 
TCTTCCTCTT CTGGCATGCA GAGCTGTGGC GAGGAGGAAG GAGAGGAGGG AGCTGACGCC 
GTCCCTCTAC CGCCCCCTAT GGCCATCCAG CAGCACAGCT TGCTGCAGCC TGACTCCCAG 
GACGACAAGT CCGGATCTCG TCTGTCCGCA CGTCCAAGCA GCTCAGAGAC GCCCAGCGCG 
GCCGAACTAG TCAGCGCCAT CGAGGAGCTG GTGAAGAGTA AGATGTCCCT GGAGGATCGC 
CCCAGCTCTC TGTCAGTAGA ACAAGGTGAC AGCAGCTCTC CCTCCCTAAA TCCCTCTGAC 
AACTCCCTCC TCTCCTCCTC CTCACCCATC GATGAGATGG ACGAGCGCAA GGCCGACTTC 
CTCAAGAGAA GACATTACGT GCTACTGGAG TTGGTGGAGA CCGAGAGGGA CTATGTCAGA 
GATCTGGGAT CAGTGGTAGA GGGCTACATG TGTAGGATGA AAGAAGAGGG TGTTCCTGAT 
GACATGAGGG GAAAAGACAA GATCGTCTTT GGAAACATCC ATCAGATCTA CGACTGGCAC 
AAAGATTTTT TCCTGGGGGA GCTTGAGAAG TGTTTAGAGG ATCCCGACAG GCTGGCACCT 
TTATTCATTA AACAGGAGCG GAGGCTGCAC ATGTACATCG TGTACTGCCA GAACAAACCC 
AAGTCTGAGC ACATCGTTTC AGAGTACATC GACACTTATT TTGAGGACCT AAAGCAGCGT 
TTGGGCCACA GGCTGCAGAT CACTGATCTA TTAATCAAGC CAGTCCAGCG TATAATGAAG 
TACCAATTAC TGCTAAAAGA TCTTTTGAAA ATGTCCAAGA AGGCTGGCTT GGATATGGCA 
GAGCTTGAGA AAGCTGTGGA GGTGATGTGC GTGGTTCCCA AACGCTGCAA CGACATGATG 
AATGTTGGAC GCCTTCAGGG TTTTGAAGGT AAAATTGTGG CGCAGGGCAG ATTACTGCTC 
CAAGATACCT TCATGGTGTC GGACCAGGAT GGGGGACTTC TGTCCAGGAT GAAGGAGAGA 
AGGGTTTTCT TATTTGAACA GATAGTAATC TTTAGCGAAC CTCTTGACAA AAAGAAGGGC 
TTCTCCACTC CAGGATACCT CTTTAAGAAC AGCATCAAGG TGAGCTGGTT GGGTTTAGAA 
GAACATTCAG AGGACCCTTG CAAGTTCACG CTTACCTCCA GATCATCTAG TGGCAACTTG 
GAGAGATACA CCCTCCATTC AATGAGTCCT GGGGTCAGTC AAGTCTGGGT TCACCAGATT 
AGCCAGATAC TGGAGAACCA GCGCAACTTC CTCAATGCTT TGACATCCCC CATTGAATAC 
CAGAGAAACC ATGTGGGTAG TGGAGTGCCA GGTGGTCTTC CCAGCAGCAG CAGCAGCAGT 
ACTGGAGGCC TACCAGGAGG CAGCAGTTCC ATTGGCTCCT CTAGCATGGG AGTAGGAGGC 
GGCGGTGGCT CTGGGGGACC TGGGAACAGC TCATCCTCCT CACTCTGCGG TCCCCGTTCC 
CGACCGTCCC GCATTCCACA GCCATCGTCA CGACTCCCAC AACCCGTCCA CCACCACCAT 
CCCCCGGGCC CTGATGGGCC TGACAGATCA GCAGGTATGT GGCCGCCCTG CCACCCTCAG 
TCCCACCCAT CTAACTCTTA TTCAGACAGG GCCACAAATG GAGAACCATT AGGCTCAGAG 
TTCCCCAAGA TGAAAAGATT GGAGCTTCAC GGAAATAACA GCAACAACAG CAACAAGTCC 
TCAGACAGTT ATGAAGACAC TCAGAAGCAC CCGGCAGCTG GTATACCGCA GATGGCTGTT 
GCGCCTCTGA ATTTGACCCT AAAAAGCCCC CGAGTCGGGA CAGTTTCTCC TCTGATTTCA 
CCTCAGTCTC CTGCAGGAGG AAAAGAAGCA TTTATCCCTT CAAGTCAAGC TCATAAAGGC 
AACTTGTTCT GGGCTGTGGT CCCTCCTATG CCTTCCTCTG TGTCGAGTCG GCCCAGTTCG 
CTCTCCTACC CCAGCGATAG TGTTGGTGGA GGGGATTCTC TGGGAAGAGG CGGCCACTGT 
ACGCCCTCTC ACCATCGCCA GTCCACTCAC AGTAAGGACA TAGACCGCAT GAGCACCTGC 
TCCTCCACCA GCGAGCAGTC CATACAGTCC ATCCAGAGCA ATGGGAGTGA AAACAGTAGC 
AGCAGCATTG TGTCCACCAT GTTGGTGACC CAGGACTATG TGGCAGTGAA GGAGGATGAG 
ATCAGTGTGG TCCAGGGTGA GGTGGTCCAA ATACTGGCCA GCAACCAACA AAACATGTTC 
CTGGTGTACC GGGCAGCCAA CGAGCACTCT CCTGCTGCTG AGGGCTGGAT CCCTGGCTAT 
GTATTAGGAC ACACCTCATC CTCTGTCACA CCTGAGCTCC CTGAAGGAAC CATCAAAAAA 
TCACTATCAT GGCACACAGC ACTCCGAATT CGCCGTAAAT CTGAAAAAAG GGACAAGGAG 
GGCAGGAAGA TCGAGAACGG GTACCGCAAA TCTCAGGACA GCCTGGCCAA CAAAGTCTCT 
GTTAAGCTTC TGAATCCCAA TTTCATTCCT GACGCACCTC CAGAATTCCT CATCCCTGTC 
ACCGATGTAG CATGTGAGAG CGGCGACACT GTGACACTCA GGTGCAAAAT CTGTAGCCGG 
CCTCGCGCCA CCGTTACCTG GCGAGGACCT GACAACGGCG TTCGGAGCAA CAACGGACGC 
TACAGCACCA CCTACAGTGA GACAGAAGAA GCGACTCTCC GTATTGTGAT GGTGTCCGTA 
GAGGACAGTG GCGTGTACAC GTGTGTCGCC ACAAATGTTG CAGGCTCCGT CACGTCCTCC 
GCCAACCTCA CAGTCTCAGC TTCGCATGAT GGCAACAGTG AGATCTGGAA GAGCAGCTTT 
GAGTTCAACT ACACAGAGAT CACAGAACTA GGAAGGGGCC GTTTCTCAGT GACCAAACGG 
TGCGACCAGA GAGGGAGCAA GCGCACGGTT GCGGCCAAAT ACGTTAGCAA GAAGCTGCTG 
CGGCGAGAGC AGGTGCTGCA GGAGATCAGA CTCCTGCAGA CTCTGGACCA TCCCAACCTG 
GTTAAGCTTC TGGACACCTA TGAAATGGCC AACAGCTATG TTCTCGTGCT AGAAATGGCG 
GACCAGGGAC GTTTCTTGGA TTACGTAGTG AGCTGGGGCA ACCTCACAGA GGAGAAGGTG 
GCACTGTACC TCAGAGAAAT TCTTGAAGCT CTTCACTACC TGCACGGCTG GAGGATAGCG 
CACCTCGACC TCAAACCTGA GAACATTGTG GTGGAACATG CATCCTCGCA GCCGGTGATC 
AAGCTGACAG ACTTTGGTGA TGCCGTGCAA CTGAATCCAG CCTCCTCGTA CATCCATCCT 
CTGCTGGGGA GCCCAGAGTT CTCTGCTCCT GAGCTGGTCC TCGGGCAGCC CTCCTCGCTG 
ACGTCGGACA TCTGGAGTTT AGGGGTGGTG ACATACATTG TACTCAGTGG CGCTTCCCCC 
TTCCTGGATG AGAGTTTGGA GGAGACGTGT CTGAACATCT GTCGCCTGGA CTTCAGCTTC 
CCGGAGGACT ACTTCCAGGG AGTGAGCCTG GCTGCCAAGG ACTTTGTCTG CCTGCTGCTC 
CAGAGCGAGC CGGAGCGGCG GCCCACTGCG TTAGCCTGCC TGCAGGAGCA GTGGCTCCAG 
CCCCGAGGTG TGATCAATAC TGGATTTGTA GGCTCGAAAC ATGCACACGC GTCGCCCAAC 
CATCACACTT TACATCTGGA CACATCCAGA CTCATTTCTT TCATTGAGAG GCGGAAACAC 
CAGAACGACA TTCGGCCCAT AGACACGGTC AGGGCCTTCC TCCACAGCCG CCTGCTTAAC 
AACATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101071578 ( XM_029839218.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029695078.1 Takifugu rubripes triple functional domain protein-like (LOC101071578), transcript variant X2, mRNA.

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CloneID	OTo69928
Clone ID Related Accession (Same CDS sequence)	XM_029839218.1
Accession Version	XM_029839218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	triple functional domain protein-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042291.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCAG GAGAGGGATT AGATGAGGCT GCGAAGGACG CGGCAGACAT AGCGGCGTTT 
TTCAAATCCG ATTGCGCTCT CCCAAAATGT GAATCCAAAC TGGACGGGCC AGGTCAAACC 
TCAGGGTTTC ACCGCAATGA GGATATGAAG GCCATTGACG TACTTCCTAT CCTCAAGGAG 
AAGGTCGCCT TTCTCTCAGG TGGCAGAGAC AGACGCGGAG GTCCCGTTCT CACATTTCCT 
GCTCGGAGCA ACCATGACAG AATACGACCT GAAGACCTCC GCAGGCTCAT AGCTTACCTG 
GCTACTATTC CCAGCGAGGA AGTGGCGAGG CATGGCTTCA CGGTCATTGT GGACATGCGT 
GGCTCTAAAT GGGACAGCAT CAAGCCTTTG CTGAAGATCC TTCAGGAGTC GTTCCCATCT 
TGCATCCACG TTGCCCTCAT CATCAAGCCC GACAACTTCT GGCAGAAGCA GAGGACAAAC 
TTCGGCAGCT CCAAATTTGA ATTCGAGACG GTGATGGTCT CTTTAGAGGG TTTATCCAAA 
ATCGTGGACC CATCACAGCT GACAGCTGAC TTTGAGGGCA GCCTGGAATA CAACCATGAT 
GACTGGATCG AGGTGCGGCT GTCCTTTGAG ACGTTTGCCA GCGATGCGGC ACGTGCGCTG 
GCACGCCTGG AGGAGCTCCA GGACACGTTG TCCCAGCGTG ATCTCCCGCG GGACCTGGAA 
GGAGCTCGGC GGCTCATGGA GGAACACGCG GCGCTCAAGA AAAGGGCCAC GAAAGCATCC 
GTGGAGGAGC TGGACACGCA GGGGCGACGG CTGCTGCAGA GGCTGCAGGC ACAGGCTACG 
GGAGGAGGCA GCGTTAATGA AAGCGGGTAC ATTAACCGCC TGGGCGGAGC TAGCGGAGAC 
CCCAACGACC ACCATCACGG GTGCCACACG CACGCAGACG CACACAACCT CATCACCAAA 
GTGAGCAGCT TGTTGGAAAA GCTTCACGGG ACTCGGCAAA ATCTGCAGCA GCTGTGGCAC 
ATGAGGAAGC TGAAGCTGGA CCAGTGTTTC CAGCTGCGTC TTTTTGAACA GGACGCAGAG 
AAGATGTTCG ACTGGATCAT GCACAACAAG GGGCTTTTTC TCACGAGTTA CACGGAGATC 
GGGGGGAACC ACCAGCACGC TGTCGAGCTG CAGACCCAAC ACAACCACTT TGCCATGAAC 
TGCATGAATG TGTACGTGAA CATCAACCGG ATCATGTCAG TCGGGAACCG GCTCCTGGAG 
TCGGGCCATT ACGCCTCGCA GCAGATCCAG CAGATCTCCG GGCAGCTGGA GCAGGAGTGG 
AAGGCCTTCG CTGCGGCGCT GGATGAACGC AGCACGCTGC TGGAGATGTC CGCCAGCTTC 
CACCAGAAAG CGGACCAGTA CATGGGGAAA GTGGAACCGT GGTGTAAAGC CTGTGGTGAA 
GGAGAGCTGC CCTCTGAACT CCAGGATCTG GAAGACACAA TCCACCATCA TCAGGGGCTT 
TATGAGCACA TCACCACTGC TTACTCTGAG GTAAGCCAGG ATGGGAAGTC ACTCTTGGAC 
AAGCTGCAGC GCCCTTTGAC CCCAGGGAGC GCTGATTCCT TGATGGCCTC TGCCAACTAC 
TCCAAGGCCG TGCATCACGT CTTAGACATC ATCCACGAGG TGCTGCACCA CCAGAGGCAG 
CTGGAAAACA TCTGGCAGCA TCGCAAAGTC CGCCTGCACC AACGGCTGCA ACTGTGTGTT 
TTTCAGCAGG ATGTGCAGCA GGTACTGGAC TGGATAGAAA ACCACGGAGA AGCCTTCCTA 
AGCAAACACA CTGGTGTTGG CAAGTCTCTG CACAGAGCCA GAGCTCTGCA GAAGAGGCAT 
GAGGATTTTG AAGAGGTTGC ACAGAACACC TACACTAATG CTGACAAACT GCTGGAAGCC 
GCAGAGCAGC TGGCTCAGAC CGGCGAATGT GATCCAGAGG AAATTTACCA GGCTGCCCAC 
CAGCTGGAAG ATCGCATCCA GGACTTCGTA CGACGTGTCG AGCAGAGAAA AGTCCTGTTG 
GACATGTCTG TCGCCTTCCA CACGCATGTC AAAGAGCTGT GGACGTGGCT GGAGGAGTTG 
CAGAAGGAAT TGCTAGATGA CGTATACGCA GAATCCGTGG AGGCTGTTCA GGACCTGATC 
AAACGCTTCG GCCAGCAGCA GCAGACCACC CTGCAGGCTA CTGTCAATGT CATCAAAGAG 
GGAGAGGACC TTATCCAACA GCTCAGAGAC TCGGCTATCT CAAGCAACAA AACTCCCCAT 
AACAGCTCCA TGGCCCACAT CGAGAGTGTG CTGCAGCAGC TGGACGAGGC TCAGGGCCAG 
ATGGAGGAAC TGTTTCAGGA AAGGAAGATC AAACTGGAGC TCTTTCTGCA GCTGCGCATC 
TTCGAGAGGG ACGCCATCGA TATCATCTCT GACCTGGAGT CATGGAACGA GGAGCTCTCC 
CAGCAGATGA GTGACTTCGA CACCGAGGAT CTGACGCTGG CGGAGCAGAG GCTGCAGCAC 
CACGCAGACA AGGCGCTCAC CATGAACAAC CTGACCTACG ACGTCATCCA CCAGGGCCAA 
GAGCTGCTGC AGTACGTGAC CGAGGTCCAG GCGTCTGGTG TGGAGCTGCT GTGCGACAGA 
GATGTGGACA TGGCCACACG GGTCCAGGAC CTGCTGGAGT TCCTCCACGA GAAGCAGCAG 
GAGCTGGACC TGGCGGCGGA GCAGCACAGG AGACACTTGG AACAGTGTGT CCAACTGAGA 
CACCTCCAGG CAGAAGTCAA ACAGGTGCTG GGATGGATCC GGAATGGCGA GTCCATGCTG 
AACGCTGGTC TGATCACAGC CAGCTCGTTA CAGGAAGCTG AACAGCTGCA GAAGGAACAT 
GAACAATTTC AGCATGCAAT AGAGAAAACT CATCAGAGCG CGCTGCAGGT GCAGCAGAAG 
GCCGAGACCT TACTCCAGGC AAACCACTAT GACATGGACA TGATCAGGGA CTGTGCAGAA 
AAGGTGGCAG ACCACTGGCA GCAGCTCATG CTGAAAATGG AAGACCGACT CAAGCTGGTC 
AACGCTTCTG TGGCCTTCTA CAAAACCTCC GAACAGGTTT GCAGTGTGTT GGAAAGCCTG 
GAGCAGGAGT ACAAGAGAGA AGAGGACTGG TGTGGTGGCG CTGATAAACT AGGCCCCAAC 
AGCGAGTCCG ACCACGTCAC TCCCATGATC AGCAAACATC TGGAGCAGAA AGAGGCCTTT 
CTCAAGGCGT GCACGCTAGC CCGGCGGAAT GCTGACGTCT TCTTGAAGTA CCTGCACAGA 
AACAGTGTCA ATATGCCCGG CATGCTGTCC CACGTCAAAG CTCCAGAGAC TCAGGTTAAG 
AATATCTTGA ACGAGCTGCT CCAGAGAGAG AACCGAGTCC TTCACTTCTG GACAATGAGG 
AAGAGGAGGC TGGACCAGTG TCAGCAATAT GTCGTATTTG AACGCAGCGC CAAACAGGCT 
CTGGAGTGGA TCCATGACAC TGGGGAGTTT TACTTGTCCA CACACACATC CACTGGCTCC 
AGCATTCATC ACACACAAGA GCTGCTGAAG GAGCATGAGG ACTTCCAGAT CACAGCAAAG 
CAAACCAAAG AGCGAGTAAA GCTGTTGATC CAGCTGGCAG ATGGGTTTTG TGACAAGGGG 
CACGCCCATG CACTGGAAAT AAAGAAATGG GTGTCTTCTG TGGACAAGCG CTACCGGGAC 
TTCTCTCTCC GCATGGACAA ATACCGTACC TGCCTGGAAA CTGCGCTCGG CATATCATCC 
GACTCCAACA AGGCTAGCAA AGAGCTCCAG CTGGATATAA TCCCAGCCAG CGCTCCGGGC 
TCAGAGGTCA AACTCCGGGA TGCTGCCCAT GACCTCAATG AGGAGAAGAG GAAGTCGGCA 
CGGAGGAAGG AATTCATAAT GGCGGAGCTG ATTCAAACAG AAAAAGCCTA CGTCAGGGAC 
CTAAGGGAGT GTTTGGATAC GTACCTGTGG GAGATGACCA GTGGTGTGGA GGAGATTCCC 
GCCGGAATTG TCAACAAAGA GCACATCATC TTTGGGAACA TGCAGGACCT CTACGAGTTC 
CACCACAACA TCTTCCTGAA GGAGTTGGAG AAGTATGAGC AACTTCCAGA AGATGTCGGC 
CATTGTTTTG TCACTTGGGC TGATAAGTTC CAGATGTACG TCAGTTACTG TAAGAACAAA 
CCCGACTCCA CGCAGCTCAT CCTCGAACAT GCCGGAGCAT ACTTCGATGA AATCCAGCAA 
AGACATCGCC TTGCTAACTC CATCTCCTCT TACCTGATCA AACCAGTGCA GAGAATCACA 
AAGTACCAGC TGCTTCTCAA GGAGCTGCTG ACATGCTGCG AGGAGGGAAA AGGTGAGATC 
AAGGACGGAC TGGAAGTGAT GCTCAGCGTC CCTAAGAAAG CCAACGACGC CATGCACCTG 
TCTATGCTGG AGGGGTTTGA TGGAAACATT GACTCCCAGG GAGAGCTGAT ACTTCAGGAA 
TCCTTCCAGG TCTGGGATCC TAAGACTCTG ATCCGAAAGG GTCGGGACCG GCACCTCTTC 
CTGTTTGAGA TGTCTCTGGT GTTAAGCAAA GAAGTCAAAG ATTCCAACGG GCGCAGCAAA 
TACCTGTACA AGAGCAAGCT GTTTACATCA GAACTTGGAG TTACGGAACA TGTGGAGGGA 
GATCCCTGTA AGTTCGCCCT GTGGGTGGGC AGGACCCCAA CGTCTGACAA CAAGATCGTC 
CTCAAGGCAT CCAGTATCGA GAACAAACAG GACTGGATCA AACACATCCG GGAGGTGATC 
CAGGAGCGGA TTGTCCACCT GCGCGGCGCA CTGAAGGAGC CCATCCACAT CCCCAAAACC 
ACCACAGCCA AACACAAAGG CAGACGGGAT GGAGAGGAAC TGGACAGCCA GGGGGATGCC 
AGCAGCCAAC CAGATACCAT CTCCATTGCC TCGCGAACAT CCCAGAACAC AATGGACAGC 
GATAAGCTCT CCGGTGGCTG TGAGTTGACA GTGGTGATCC ACGACTTCGT GGCCAGTAAT 
GGCAGCAGCG GCGAGCTGTC GGTGCGCCGA GGTCAGACTG TGGAAGTCCT GGAACGGCTC 
CACGACAAGC CCGACTGGTG CCTGGTGCGG ACCACGGACC GTTCCCCGGC TCAGGAGGGC 
ATCGTTCCCT GCTCCATGCT CTGCATCGCT CACTCGAGGT CTTCCATGGA GATGGAAGGG 
CTCTTTAACC ACAAAGACAC ATTATCTGTA TCCAGTAACG ACGCCCTCCA ACCAGGCTCC 
AGCCAAACCC TGGGTCCTCA CAGCTCCCCC GGCCCCAAAC GCCCAGGGAA CACACTGAGG 
AAGTGGCTGA CCAGCCCCGT GCGGAGGCTC AGCTCTGGCA AGGCCGACGG CCACGTGAAG 
AAACTGGCCC ACAAGCATAA GAAGAACCGC GAGGGCGCGA GGAAGAACAT GGATGCCATG 
CACGGTTCTC AGAAGGACTC TGATGACAGC GCCGCTACGC CCCAGGATGA AACGCTAGAG 
GAACGTATGC GTAACGAAGG GCTGAGCAGC GGCACCCTGT CCAAGTCTTC CTCTTCTGGC 
ATGCAGAGCT GTGGCGAGGA GGAAGGAGAG GAGGGAGCCG ACGCCGTCCC TCTACCGCCC 
CCTATGGCCA TCCAGCAGCA CAGCTTGCTG CAGCCTGACT CCCAGGACGA CAAGTCCGGA 
TCTCGTCTGT CCGCACGTCC AAGCAGCTCA GAGACGCCCA GCGCGGCCGA ACTAGTCAGC 
GCCATCGAGG AGCTGGTGAA GAGTAAGATG TCCCTGGAGG ATCGTCCCAG CTCTCTGTCA 
GTAGAACAAG GTGACAGCAG CTCTCCCTCC CTAAATCCCT CTGACAACTC CCTCCTCTCC 
TCCTCCTCAC CCATCGATGA GATGGACGAG CGCAAGGCCG ACTTCCTCAA GAGAAGACAT 
TACGTGCTAC TGGAGTTGGT GGAGACCGAG AGGGACTATG TCAGAGATCT GGGATCAGTG 
GTAGAGGGCT ACATGTGTAG GATGAAAGAA GAGGGTGTTC CTGATGACAT GAGGGGAAAA 
GACAAGATCG TCTTTGGAAA CATCCATCAG ATCTACGACT GGCACAAAGA TTTTTTCCTG 
GGGGAGCTTG AGAAGTGTTT AGAGGATCCC GACAGGCTGG CACCTTTATT CATTAAACAG 
GAGCGGAGGC TGCACATGTA CATCGTGTAC TGCCAGAACA AACCCAAGTC TGAGCACATC 
GTTTCAGAGT ACATCGACAC TTATTTTGAG GACCTAAAGC AGCGTTTGGG CCACAGGCTG 
CAGATCACTG ATCTATTAAT CAAGCCAGTC CAGCGTATAA TGAAGTACCA ATTACTGCTA 
AAAGATCTTT TGAAAATGTC CAAGAAGGCT GGCTTGGATA TGGCAGAGCT TGAGAAAGCT 
GTGGAGGTGA TGTGCGTGGT TCCCAAACGC TGCAACGACA TGATGAATGT TGGACGCCTT 
CAGGGTTTTG AAGGTAAAAT TGTGGCGCAG GGCAGATTAC TGCTCCAAGA TACCTTCATG 
GTGTCGGACC AGGATGGGGG ACTTCTGTCC AGGATGAAGG AGAGAAGGGT TTTCTTATTT 
GAACAGATAG TAATCTTTAG CGAACCTCTT GACAAAAAGA AGGGCTTCTC CACTCCAGGA 
TACCTCTTTA AGAACAGCAT CAAGGTGAGC TGGTTGGGTT TAGAAGAACA TTCAGAGGAC 
CCTTGCAAGT TCACGCTTAC CTCCAGATCA TCTAGTGGCA ACTTGGAGAG ATACACCCTC 
CATTCAATGA GTCCTGGGGT CAGTCAAGTC TGGGTTCACC AGATTAGCCA GATACTGGAG 
AACCAGCGCA ACTTCCTCAA TGCTTTGACA TCCCCCATTG AATACCAGAG AAACCATGTG 
GGTAGTGGAG TGCCAGGTGG TCTTCCCAGC AGCAGCAGCA GCAGTACTGG AGGCCTACCA 
GGAGGCAGCA GTTCCATTGG CTCCTCTAGC ATGGGAGTAG GAGGCGGCGG TGGCTCTGGG 
GGACCTGGGA ACAGCTCATC CTCCTCACTC TGCGGTCCCC GTTCCCGACC GTCCCGCATT 
CCACAGCCAT CGTCACGACT CCCACAACCC GTCCACCACC ACCATCCCCC GGGCCCTGAT 
GGGCCTGACA GATCAGCAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029695078.1
CDS	203..7366
Translation	MSAGEGLDEAAKDAADIAAFFKSDCALPKCESKLDGPGQTSGFHRNEDMKAIDVLPILKEKVAFLSGGRDRRGGPVLTFPARSNHDRIRPEDLRRLIAYLATIPSEEVARHGFTVIVDMRGSKWDSIKPLLKILQESFPSCIHVALIIKPDNFWQKQRTNFGSSKFEFETVMVSLEGLSKIVDPSQLTADFEGSLEYNHDDWIEVRLSFETFASDAARALARLEELQDTLSQRDLPRDLEGARRLMEEHAALKKRATKASVEELDTQGRRLLQRLQAQATGGGSVNESGYINRLGGASGDPNDHHHGCHTHADAHNLITKVSSLLEKLHGTRQNLQQLWHMRKLKLDQCFQLRLFEQDAEKMFDWIMHNKGLFLTSYTEIGGNHQHAVELQTQHNHFAMNCMNVYVNINRIMSVGNRLLESGHYASQQIQQISGQLEQEWKAFAAALDERSTLLEMSASFHQKADQYMGKVEPWCKACGEGELPSELQDLEDTIHHHQGLYEHITTAYSEVSQDGKSLLDKLQRPLTPGSADSLMASANYSKAVHHVLDIIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKVLLDMSVAFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQATVNVIKEGEDLIQQLRDSAISSNKTPHNSSMAHIESVLQQLDEAQGQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNEELSQQMSDFDTEDLTLAEQRLQHHADKALTMNNLTYDVIHQGQELLQYVTEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRRHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQKEHEQFQHAIEKTHQSALQVQQKAETLLQANHYDMDMIRDCAEKVADHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSESDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMLSHVKAPETQVKNILNELLQRENRVLHFWTMRKRRLDQCQQYVVFERSAKQALEWIHDTGEFYLSTHTSTGSSIHHTQELLKEHEDFQITAKQTKERVKLLIQLADGFCDKGHAHALEIKKWVSSVDKRYRDFSLRMDKYRTCLETALGISSDSNKASKELQLDIIPASAPGSEVKLRDAAHDLNEEKRKSARRKEFIMAELIQTEKAYVRDLRECLDTYLWEMTSGVEEIPAGIVNKEHIIFGNMQDLYEFHHNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVSYCKNKPDSTQLILEHAGAYFDEIQQRHRLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKKANDAMHLSMLEGFDGNIDSQGELILQESFQVWDPKTLIRKGRDRHLFLFEMSLVLSKEVKDSNGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERIVHLRGALKEPIHIPKTTTAKHKGRRDGEELDSQGDASSQPDTISIASRTSQNTMDSDKLSGGCELTVVIHDFVASNGSSGELSVRRGQTVEVLERLHDKPDWCLVRTTDRSPAQEGIVPCSMLCIAHSRSSMEMEGLFNHKDTLSVSSNDALQPGSSQTLGPHSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKNREGARKNMDAMHGSQKDSDDSAATPQDETLEERMRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKSGSRLSARPSSSETPSAAELVSAIEELVKSKMSLEDRPSSLSVEQGDSSSPSLNPSDNSLLSSSSPIDEMDERKADFLKRRHYVLLELVETERDYVRDLGSVVEGYMCRMKEEGVPDDMRGKDKIVFGNIHQIYDWHKDFFLGELEKCLEDPDRLAPLFIKQERRLHMYIVYCQNKPKSEHIVSEYIDTYFEDLKQRLGHRLQITDLLIKPVQRIMKYQLLLKDLLKMSKKAGLDMAELEKAVEVMCVVPKRCNDMMNVGRLQGFEGKIVAQGRLLLQDTFMVSDQDGGLLSRMKERRVFLFEQIVIFSEPLDKKKGFSTPGYLFKNSIKVSWLGLEEHSEDPCKFTLTSRSSSGNLERYTLHSMSPGVSQVWVHQISQILENQRNFLNALTSPIEYQRNHVGSGVPGGLPSSSSSSTGGLPGGSSSIGSSSMGVGGGGGSGGPGNSSSSSLCGPRSRPSRIPQPSSRLPQPVHHHHPPGPDGPDRSAE

Target ORF information:

RefSeq Version	XM_029839218.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes triple functional domain protein-like (LOC101071578), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029839218.1

ORF Insert Sequence:

ATGAGCGCAG GAGAGGGATT AGATGAGGCT GCGAAGGACG CGGCAGACAT AGCGGCGTTT 
TTCAAATCCG ATTGCGCTCT CCCAAAATGT GAATCCAAAC TGGACGGGCC AGGTCAAACC 
TCAGGGTTTC ACCGCAATGA GGATATGAAG GCCATTGACG TACTTCCTAT CCTCAAGGAG 
AAGGTCGCCT TTCTCTCAGG TGGCAGAGAC AGACGCGGAG GTCCCGTTCT CACATTTCCT 
GCTCGGAGCA ACCATGACAG AATACGACCT GAAGACCTCC GCAGGCTCAT AGCTTACCTG 
GCTACTATTC CCAGCGAGGA AGTGGCGAGG CATGGCTTCA CGGTCATTGT GGACATGCGT 
GGCTCTAAAT GGGACAGCAT CAAGCCTTTG CTGAAGATCC TTCAGGAGTC GTTCCCATCT 
TGCATCCACG TTGCCCTCAT CATCAAGCCC GACAACTTCT GGCAGAAGCA GAGGACAAAC 
TTCGGCAGCT CCAAATTTGA ATTCGAGACG GTGATGGTCT CTTTAGAGGG TTTATCCAAA 
ATCGTGGACC CATCACAGCT GACAGCTGAC TTTGAGGGCA GCCTGGAATA CAACCATGAT 
GACTGGATCG AGGTGCGGCT GTCCTTTGAG ACGTTTGCCA GCGATGCGGC ACGTGCGCTG 
GCACGCCTGG AGGAGCTCCA GGACACGTTG TCCCAGCGTG ATCTCCCGCG GGACCTGGAA 
GGAGCTCGGC GGCTCATGGA GGAACACGCG GCGCTCAAGA AAAGGGCCAC GAAAGCATCC 
GTGGAGGAGC TGGACACGCA GGGGCGACGG CTGCTGCAGA GGCTGCAGGC ACAGGCTACG 
GGAGGAGGCA GCGTTAATGA AAGCGGGTAC ATTAACCGCC TGGGCGGAGC TAGCGGAGAC 
CCCAACGACC ACCATCACGG GTGCCACACG CACGCAGACG CACACAACCT CATCACCAAA 
GTGAGCAGCT TGTTGGAAAA GCTTCACGGG ACTCGGCAAA ATCTGCAGCA GCTGTGGCAC 
ATGAGGAAGC TGAAGCTGGA CCAGTGTTTC CAGCTGCGTC TTTTTGAACA GGACGCAGAG 
AAGATGTTCG ACTGGATCAT GCACAACAAG GGGCTTTTTC TCACGAGTTA CACGGAGATC 
GGGGGGAACC ACCAGCACGC TGTCGAGCTG CAGACCCAAC ACAACCACTT TGCCATGAAC 
TGCATGAATG TGTACGTGAA CATCAACCGG ATCATGTCAG TCGGGAACCG GCTCCTGGAG 
TCGGGCCATT ACGCCTCGCA GCAGATCCAG CAGATCTCCG GGCAGCTGGA GCAGGAGTGG 
AAGGCCTTCG CTGCGGCGCT GGATGAACGC AGCACGCTGC TGGAGATGTC CGCCAGCTTC 
CACCAGAAAG CGGACCAGTA CATGGGGAAA GTGGAACCGT GGTGTAAAGC CTGTGGTGAA 
GGAGAGCTGC CCTCTGAACT CCAGGATCTG GAAGACACAA TCCACCATCA TCAGGGGCTT 
TATGAGCACA TCACCACTGC TTACTCTGAG GTAAGCCAGG ATGGGAAGTC ACTCTTGGAC 
AAGCTGCAGC GCCCTTTGAC CCCAGGGAGC GCTGATTCCT TGATGGCCTC TGCCAACTAC 
TCCAAGGCCG TGCATCACGT CTTAGACATC ATCCACGAGG TGCTGCACCA CCAGAGGCAG 
CTGGAAAACA TCTGGCAGCA TCGCAAAGTC CGCCTGCACC AACGGCTGCA ACTGTGTGTT 
TTTCAGCAGG ATGTGCAGCA GGTACTGGAC TGGATAGAAA ACCACGGAGA AGCCTTCCTA 
AGCAAACACA CTGGTGTTGG CAAGTCTCTG CACAGAGCCA GAGCTCTGCA GAAGAGGCAT 
GAGGATTTTG AAGAGGTTGC ACAGAACACC TACACTAATG CTGACAAACT GCTGGAAGCC 
GCAGAGCAGC TGGCTCAGAC CGGCGAATGT GATCCAGAGG AAATTTACCA GGCTGCCCAC 
CAGCTGGAAG ATCGCATCCA GGACTTCGTA CGACGTGTCG AGCAGAGAAA AGTCCTGTTG 
GACATGTCTG TCGCCTTCCA CACGCATGTC AAAGAGCTGT GGACGTGGCT GGAGGAGTTG 
CAGAAGGAAT TGCTAGATGA CGTATACGCA GAATCCGTGG AGGCTGTTCA GGACCTGATC 
AAACGCTTCG GCCAGCAGCA GCAGACCACC CTGCAGGCTA CTGTCAATGT CATCAAAGAG 
GGAGAGGACC TTATCCAACA GCTCAGAGAC TCGGCTATCT CAAGCAACAA AACTCCCCAT 
AACAGCTCCA TGGCCCACAT CGAGAGTGTG CTGCAGCAGC TGGACGAGGC TCAGGGCCAG 
ATGGAGGAAC TGTTTCAGGA AAGGAAGATC AAACTGGAGC TCTTTCTGCA GCTGCGCATC 
TTCGAGAGGG ACGCCATCGA TATCATCTCT GACCTGGAGT CATGGAACGA GGAGCTCTCC 
CAGCAGATGA GTGACTTCGA CACCGAGGAT CTGACGCTGG CGGAGCAGAG GCTGCAGCAC 
CACGCAGACA AGGCGCTCAC CATGAACAAC CTGACCTACG ACGTCATCCA CCAGGGCCAA 
GAGCTGCTGC AGTACGTGAC CGAGGTCCAG GCGTCTGGTG TGGAGCTGCT GTGCGACAGA 
GATGTGGACA TGGCCACACG GGTCCAGGAC CTGCTGGAGT TCCTCCACGA GAAGCAGCAG 
GAGCTGGACC TGGCGGCGGA GCAGCACAGG AGACACTTGG AACAGTGTGT CCAACTGAGA 
CACCTCCAGG CAGAAGTCAA ACAGGTGCTG GGATGGATCC GGAATGGCGA GTCCATGCTG 
AACGCTGGTC TGATCACAGC CAGCTCGTTA CAGGAAGCTG AACAGCTGCA GAAGGAACAT 
GAACAATTTC AGCATGCAAT AGAGAAAACT CATCAGAGCG CGCTGCAGGT GCAGCAGAAG 
GCCGAGACCT TACTCCAGGC AAACCACTAT GACATGGACA TGATCAGGGA CTGTGCAGAA 
AAGGTGGCAG ACCACTGGCA GCAGCTCATG CTGAAAATGG AAGACCGACT CAAGCTGGTC 
AACGCTTCTG TGGCCTTCTA CAAAACCTCC GAACAGGTTT GCAGTGTGTT GGAAAGCCTG 
GAGCAGGAGT ACAAGAGAGA AGAGGACTGG TGTGGTGGCG CTGATAAACT AGGCCCCAAC 
AGCGAGTCCG ACCACGTCAC TCCCATGATC AGCAAACATC TGGAGCAGAA AGAGGCCTTT 
CTCAAGGCGT GCACGCTAGC CCGGCGGAAT GCTGACGTCT TCTTGAAGTA CCTGCACAGA 
AACAGTGTCA ATATGCCCGG CATGCTGTCC CACGTCAAAG CTCCAGAGAC TCAGGTTAAG 
AATATCTTGA ACGAGCTGCT CCAGAGAGAG AACCGAGTCC TTCACTTCTG GACAATGAGG 
AAGAGGAGGC TGGACCAGTG TCAGCAATAT GTCGTATTTG AACGCAGCGC CAAACAGGCT 
CTGGAGTGGA TCCATGACAC TGGGGAGTTT TACTTGTCCA CACACACATC CACTGGCTCC 
AGCATTCATC ACACACAAGA GCTGCTGAAG GAGCATGAGG ACTTCCAGAT CACAGCAAAG 
CAAACCAAAG AGCGAGTAAA GCTGTTGATC CAGCTGGCAG ATGGGTTTTG TGACAAGGGG 
CACGCCCATG CACTGGAAAT AAAGAAATGG GTGTCTTCTG TGGACAAGCG CTACCGGGAC 
TTCTCTCTCC GCATGGACAA ATACCGTACC TGCCTGGAAA CTGCGCTCGG CATATCATCC 
GACTCCAACA AGGCTAGCAA AGAGCTCCAG CTGGATATAA TCCCAGCCAG CGCTCCGGGC 
TCAGAGGTCA AACTCCGGGA TGCTGCCCAT GACCTCAATG AGGAGAAGAG GAAGTCGGCA 
CGGAGGAAGG AATTCATAAT GGCGGAGCTG ATTCAAACAG AAAAAGCCTA CGTCAGGGAC 
CTAAGGGAGT GTTTGGATAC GTACCTGTGG GAGATGACCA GTGGTGTGGA GGAGATTCCC 
GCCGGAATTG TCAACAAAGA GCACATCATC TTTGGGAACA TGCAGGACCT CTACGAGTTC 
CACCACAACA TCTTCCTGAA GGAGTTGGAG AAGTATGAGC AACTTCCAGA AGATGTCGGC 
CATTGTTTTG TCACTTGGGC TGATAAGTTC CAGATGTACG TCAGTTACTG TAAGAACAAA 
CCCGACTCCA CGCAGCTCAT CCTCGAACAT GCCGGAGCAT ACTTCGATGA AATCCAGCAA 
AGACATCGCC TTGCTAACTC CATCTCCTCT TACCTGATCA AACCAGTGCA GAGAATCACA 
AAGTACCAGC TGCTTCTCAA GGAGCTGCTG ACATGCTGCG AGGAGGGAAA AGGTGAGATC 
AAGGACGGAC TGGAAGTGAT GCTCAGCGTC CCTAAGAAAG CCAACGACGC CATGCACCTG 
TCTATGCTGG AGGGGTTTGA TGGAAACATT GACTCCCAGG GAGAGCTGAT ACTTCAGGAA 
TCCTTCCAGG TCTGGGATCC TAAGACTCTG ATCCGAAAGG GTCGGGACCG GCACCTCTTC 
CTGTTTGAGA TGTCTCTGGT GTTAAGCAAA GAAGTCAAAG ATTCCAACGG GCGCAGCAAA 
TACCTGTACA AGAGCAAGCT GTTTACATCA GAACTTGGAG TTACGGAACA TGTGGAGGGA 
GATCCCTGTA AGTTCGCCCT GTGGGTGGGC AGGACCCCAA CGTCTGACAA CAAGATCGTC 
CTCAAGGCAT CCAGTATCGA GAACAAACAG GACTGGATCA AACACATCCG GGAGGTGATC 
CAGGAGCGGA TTGTCCACCT GCGCGGCGCA CTGAAGGAGC CCATCCACAT CCCCAAAACC 
ACCACAGCCA AACACAAAGG CAGACGGGAT GGAGAGGAAC TGGACAGCCA GGGGGATGCC 
AGCAGCCAAC CAGATACCAT CTCCATTGCC TCGCGAACAT CCCAGAACAC AATGGACAGC 
GATAAGCTCT CCGGTGGCTG TGAGTTGACA GTGGTGATCC ACGACTTCGT GGCCAGTAAT 
GGCAGCAGCG GCGAGCTGTC GGTGCGCCGA GGTCAGACTG TGGAAGTCCT GGAACGGCTC 
CACGACAAGC CCGACTGGTG CCTGGTGCGG ACCACGGACC GTTCCCCGGC TCAGGAGGGC 
ATCGTTCCCT GCTCCATGCT CTGCATCGCT CACTCGAGGT CTTCCATGGA GATGGAAGGG 
CTCTTTAACC ACAAAGACAC ATTATCTGTA TCCAGTAACG ACGCCCTCCA ACCAGGCTCC 
AGCCAAACCC TGGGTCCTCA CAGCTCCCCC GGCCCCAAAC GCCCAGGGAA CACACTGAGG 
AAGTGGCTGA CCAGCCCCGT GCGGAGGCTC AGCTCTGGCA AGGCCGACGG CCACGTGAAG 
AAACTGGCCC ACAAGCATAA GAAGAACCGC GAGGGCGCGA GGAAGAACAT GGATGCCATG 
CACGGTTCTC AGAAGGACTC TGATGACAGC GCCGCTACGC CCCAGGATGA AACGCTAGAG 
GAACGTATGC GTAACGAAGG GCTGAGCAGC GGCACCCTGT CCAAGTCTTC CTCTTCTGGC 
ATGCAGAGCT GTGGCGAGGA GGAAGGAGAG GAGGGAGCCG ACGCCGTCCC TCTACCGCCC 
CCTATGGCCA TCCAGCAGCA CAGCTTGCTG CAGCCTGACT CCCAGGACGA CAAGTCCGGA 
TCTCGTCTGT CCGCACGTCC AAGCAGCTCA GAGACGCCCA GCGCGGCCGA ACTAGTCAGC 
GCCATCGAGG AGCTGGTGAA GAGTAAGATG TCCCTGGAGG ATCGTCCCAG CTCTCTGTCA 
GTAGAACAAG GTGACAGCAG CTCTCCCTCC CTAAATCCCT CTGACAACTC CCTCCTCTCC 
TCCTCCTCAC CCATCGATGA GATGGACGAG CGCAAGGCCG ACTTCCTCAA GAGAAGACAT 
TACGTGCTAC TGGAGTTGGT GGAGACCGAG AGGGACTATG TCAGAGATCT GGGATCAGTG 
GTAGAGGGCT ACATGTGTAG GATGAAAGAA GAGGGTGTTC CTGATGACAT GAGGGGAAAA 
GACAAGATCG TCTTTGGAAA CATCCATCAG ATCTACGACT GGCACAAAGA TTTTTTCCTG 
GGGGAGCTTG AGAAGTGTTT AGAGGATCCC GACAGGCTGG CACCTTTATT CATTAAACAG 
GAGCGGAGGC TGCACATGTA CATCGTGTAC TGCCAGAACA AACCCAAGTC TGAGCACATC 
GTTTCAGAGT ACATCGACAC TTATTTTGAG GACCTAAAGC AGCGTTTGGG CCACAGGCTG 
CAGATCACTG ATCTATTAAT CAAGCCAGTC CAGCGTATAA TGAAGTACCA ATTACTGCTA 
AAAGATCTTT TGAAAATGTC CAAGAAGGCT GGCTTGGATA TGGCAGAGCT TGAGAAAGCT 
GTGGAGGTGA TGTGCGTGGT TCCCAAACGC TGCAACGACA TGATGAATGT TGGACGCCTT 
CAGGGTTTTG AAGGTAAAAT TGTGGCGCAG GGCAGATTAC TGCTCCAAGA TACCTTCATG 
GTGTCGGACC AGGATGGGGG ACTTCTGTCC AGGATGAAGG AGAGAAGGGT TTTCTTATTT 
GAACAGATAG TAATCTTTAG CGAACCTCTT GACAAAAAGA AGGGCTTCTC CACTCCAGGA 
TACCTCTTTA AGAACAGCAT CAAGGTGAGC TGGTTGGGTT TAGAAGAACA TTCAGAGGAC 
CCTTGCAAGT TCACGCTTAC CTCCAGATCA TCTAGTGGCA ACTTGGAGAG ATACACCCTC 
CATTCAATGA GTCCTGGGGT CAGTCAAGTC TGGGTTCACC AGATTAGCCA GATACTGGAG 
AACCAGCGCA ACTTCCTCAA TGCTTTGACA TCCCCCATTG AATACCAGAG AAACCATGTG 
GGTAGTGGAG TGCCAGGTGG TCTTCCCAGC AGCAGCAGCA GCAGTACTGG AGGCCTACCA 
GGAGGCAGCA GTTCCATTGG CTCCTCTAGC ATGGGAGTAG GAGGCGGCGG TGGCTCTGGG 
GGACCTGGGA ACAGCTCATC CTCCTCACTC TGCGGTCCCC GTTCCCGACC GTCCCGCATT 
CCACAGCCAT CGTCACGACT CCCACAACCC GTCCACCACC ACCATCCCCC GGGCCCTGAT 
GGGCCTGACA GATCAGCAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101071578 ( XM_029839217.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029695077.1 Takifugu rubripes triple functional domain protein-like (LOC101071578), transcript variant X1, mRNA.

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CloneID	OTo69927
Clone ID Related Accession (Same CDS sequence)	XM_029839217.1
Accession Version	XM_029839217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	triple functional domain protein-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042291.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCAG GAGAGGGATT AGATGAGGCT GCGAAGGACG CGGCAGACAT AGCGGCGTTT 
TTCAAATCCG ATTGCGCTCT CCCAAAATGT GAATCCAAAC TGGACGGGCC AGGTCAAACC 
TCAGGGTTTC ACCGCAATGA GGATATGAAG GCCATTGACG TACTTCCTAT CCTCAAGGAG 
AAGGTCGCCT TTCTCTCAGG TGGCAGAGAC AGACGCGGAG GTCCCGTTCT CACATTTCCT 
GCTCGGAGCA ACCATGACAG AATACGACCT GAAGACCTCC GCAGGCTCAT AGCTTACCTG 
GCTACTATTC CCAGCGAGGA AGTGGCGAGG CATGGCTTCA CGGTCATTGT GGACATGCGT 
GGCTCTAAAT GGGACAGCAT CAAGCCTTTG CTGAAGATCC TTCAGGAGTC GTTCCCATCT 
TGCATCCACG TTGCCCTCAT CATCAAGCCC GACAACTTCT GGCAGAAGCA GAGGACAAAC 
TTCGGCAGCT CCAAATTTGA ATTCGAGACG GTGATGGTCT CTTTAGAGGG TTTATCCAAA 
ATCGTGGACC CATCACAGCT GACAGCTGAC TTTGAGGGCA GCCTGGAATA CAACCATGAT 
GACTGGATCG AGGTGCGGCT GTCCTTTGAG ACGTTTGCCA GCGATGCGGC ACGTGCGCTG 
GCACGCCTGG AGGAGCTCCA GGACACGTTG TCCCAGCGTG ATCTCCCGCG GGACCTGGAA 
GGAGCTCGGC GGCTCATGGA GGAACACGCG GCGCTCAAGA AAAGGGCCAC GAAAGCATCC 
GTGGAGGAGC TGGACACGCA GGGGCGACGG CTGCTGCAGA GGCTGCAGGC ACAGGCTACG 
GGAGGAGGCA GCGTTAATGA AAGCGGGTAC ATTAACCGCC TGGGCGGAGC TAGCGGAGAC 
CCCAACGACC ACCATCACGG GTGCCACACG CACGCAGACG CACACAACCT CATCACCAAA 
GTGAGCAGCT TGTTGGAAAA GCTTCACGGG ACTCGGCAAA ATCTGCAGCA GCTGTGGCAC 
ATGAGGAAGC TGAAGCTGGA CCAGTGTTTC CAGCTGCGTC TTTTTGAACA GGACGCAGAG 
AAGATGTTCG ACTGGATCAT GCACAACAAG GGGCTTTTTC TCACGAGTTA CACGGAGATC 
GGGGGGAACC ACCAGCACGC TGTCGAGCTG CAGACCCAAC ACAACCACTT TGCCATGAAC 
TGCATGAATG TGTACGTGAA CATCAACCGG ATCATGTCAG TCGGGAACCG GCTCCTGGAG 
TCGGGCCATT ACGCCTCGCA GCAGATCCAG CAGATCTCCG GGCAGCTGGA GCAGGAGTGG 
AAGGCCTTCG CTGCGGCGCT GGATGAACGC AGCACGCTGC TGGAGATGTC CGCCAGCTTC 
CACCAGAAAG CGGACCAGTA CATGGGGAAA GTGGAACCGT GGTGTAAAGC CTGTGGTGAA 
GGAGAGCTGC CCTCTGAACT CCAGGATCTG GAAGACACAA TCCACCATCA TCAGGGGCTT 
TATGAGCACA TCACCACTGC TTACTCTGAG GTAAGCCAGG ATGGGAAGTC ACTCTTGGAC 
AAGCTGCAGC GCCCTTTGAC CCCAGGGAGC GCTGATTCCT TGATGGCCTC TGCCAACTAC 
TCCAAGGCCG TGCATCACGT CTTAGACATC ATCCACGAGG TGCTGCACCA CCAGAGGCAG 
CTGGAAAACA TCTGGCAGCA TCGCAAAGTC CGCCTGCACC AACGGCTGCA ACTGTGTGTT 
TTTCAGCAGG ATGTGCAGCA GGTACTGGAC TGGATAGAAA ACCACGGAGA AGCCTTCCTA 
AGCAAACACA CTGGTGTTGG CAAGTCTCTG CACAGAGCCA GAGCTCTGCA GAAGAGGCAT 
GAGGATTTTG AAGAGGTTGC ACAGAACACC TACACTAATG CTGACAAACT GCTGGAAGCC 
GCAGAGCAGC TGGCTCAGAC CGGCGAATGT GATCCAGAGG AAATTTACCA GGCTGCCCAC 
CAGCTGGAAG ATCGCATCCA GGACTTCGTA CGACGTGTCG AGCAGAGAAA AGTCCTGTTG 
GACATGTCTG TCGCCTTCCA CACGCATGTC AAAGAGCTGT GGACGTGGCT GGAGGAGTTG 
CAGAAGGAAT TGCTAGATGA CGTATACGCA GAATCCGTGG AGGCTGTTCA GGACCTGATC 
AAACGCTTCG GCCAGCAGCA GCAGACCACC CTGCAGGCTA CTGTCAATGT CATCAAAGAG 
GGAGAGGACC TTATCCAACA GCTCAGAGAC TCGGCTATCT CAAGCAACAA AACTCCCCAT 
AACAGCTCCA TGGCCCACAT CGAGAGTGTG CTGCAGCAGC TGGACGAGGC TCAGGGCCAG 
ATGGAGGAAC TGTTTCAGGA AAGGAAGATC AAACTGGAGC TCTTTCTGCA GCTGCGCATC 
TTCGAGAGGG ACGCCATCGA TATCATCTCT GACCTGGAGT CATGGAACGA GGAGCTCTCC 
CAGCAGATGA GTGACTTCGA CACCGAGGAT CTGACGCTGG CGGAGCAGAG GCTGCAGCAC 
CACGCAGACA AGGCGCTCAC CATGAACAAC CTGACCTACG ACGTCATCCA CCAGGGCCAA 
GAGCTGCTGC AGTACGTGAC CGAGGTCCAG GCGTCTGGTG TGGAGCTGCT GTGCGACAGA 
GATGTGGACA TGGCCACACG GGTCCAGGAC CTGCTGGAGT TCCTCCACGA GAAGCAGCAG 
GAGCTGGACC TGGCGGCGGA GCAGCACAGG AGACACTTGG AACAGTGTGT CCAACTGAGA 
CACCTCCAGG CAGAAGTCAA ACAGGTGCTG GGATGGATCC GGAATGGCGA GTCCATGCTG 
AACGCTGGTC TGATCACAGC CAGCTCGTTA CAGGAAGCTG AACAGCTGCA GAAGGAACAT 
GAACAATTTC AGCATGCAAT AGAGAAAACT CATCAGAGCG CGCTGCAGGT GCAGCAGAAG 
GCCGAGACCT TACTCCAGGC AAACCACTAT GACATGGACA TGATCAGGGA CTGTGCAGAA 
AAGGTGGCAG ACCACTGGCA GCAGCTCATG CTGAAAATGG AAGACCGACT CAAGCTGGTC 
AACGCTTCTG TGGCCTTCTA CAAAACCTCC GAACAGGTTT GCAGTGTGTT GGAAAGCCTG 
GAGCAGGAGT ACAAGAGAGA AGAGGACTGG TGTGGTGGCG CTGATAAACT AGGCCCCAAC 
AGCGAGTCCG ACCACGTCAC TCCCATGATC AGCAAACATC TGGAGCAGAA AGAGGCCTTT 
CTCAAGGCGT GCACGCTAGC CCGGCGGAAT GCTGACGTCT TCTTGAAGTA CCTGCACAGA 
AACAGTGTCA ATATGCCCGG CATGCTGTCC CACGTCAAAG CTCCAGAGAC TCAGGTTAAG 
AATATCTTGA ACGAGCTGCT CCAGAGAGAG AACCGAGTCC TTCACTTCTG GACAATGAGG 
AAGAGGAGGC TGGACCAGTG TCAGCAATAT GTCGTATTTG AACGCAGCGC CAAACAGGCT 
CTGGAGTGGA TCCATGACAC TGGGGAGTTT TACTTGTCCA CACACACATC CACTGGCTCC 
AGCATTCATC ACACACAAGA GCTGCTGAAG GAGCATGAGG ACTTCCAGAT CACAGCAAAG 
CAAACCAAAG AGCGAGTAAA GCTGTTGATC CAGCTGGCAG ATGGGTTTTG TGACAAGGGG 
CACGCCCATG CACTGGAAAT AAAGAAATGG GTGTCTTCTG TGGACAAGCG CTACCGGGAC 
TTCTCTCTCC GCATGGACAA ATACCGTACC TGCCTGGAAA CTGCGCTCGG CATATCATCC 
GACTCCAACA AGGCTAGCAA AGAGCTCCAG CTGGATATAA TCCCAGCCAG CGCTCCGGGC 
TCAGAGGTCA AACTCCGGGA TGCTGCCCAT GACCTCAATG AGGAGAAGAG GAAGTCGGCA 
CGGAGGAAGG AATTCATAAT GGCGGAGCTG ATTCAAACAG AAAAAGCCTA CGTCAGGGAC 
CTAAGGGAGT GTTTGGATAC GTACCTGTGG GAGATGACCA GTGGTGTGGA GGAGATTCCC 
GCCGGAATTG TCAACAAAGA GCACATCATC TTTGGGAACA TGCAGGACCT CTACGAGTTC 
CACCACAACA TCTTCCTGAA GGAGTTGGAG AAGTATGAGC AACTTCCAGA AGATGTCGGC 
CATTGTTTTG TCACTTGGGC TGATAAGTTC CAGATGTACG TCAGTTACTG TAAGAACAAA 
CCCGACTCCA CGCAGCTCAT CCTCGAACAT GCCGGAGCAT ACTTCGATGA AATCCAGCAA 
AGACATCGCC TTGCTAACTC CATCTCCTCT TACCTGATCA AACCAGTGCA GAGAATCACA 
AAGTACCAGC TGCTTCTCAA GGAGCTGCTG ACATGCTGCG AGGAGGGAAA AGGTGAGATC 
AAGGACGGAC TGGAAGTGAT GCTCAGCGTC CCTAAGAAAG CCAACGACGC CATGCACCTG 
TCTATGCTGG AGGGGTTTGA TGGAAACATT GACTCCCAGG GAGAGCTGAT ACTTCAGGAA 
TCCTTCCAGG TCTGGGATCC TAAGACTCTG ATCCGAAAGG GTCGGGACCG GCACCTCTTC 
CTGTTTGAGA TGTCTCTGGT GTTAAGCAAA GAAGTCAAAG ATTCCAACGG GCGCAGCAAA 
TACCTGTACA AGAGCAAGCT GTTTACATCA GAACTTGGAG TTACGGAACA TGTGGAGGGA 
GATCCCTGTA AGTTCGCCCT GTGGGTGGGC AGGACCCCAA CGTCTGACAA CAAGATCGTC 
CTCAAGGCAT CCAGTATCGA GAACAAACAG GACTGGATCA AACACATCCG GGAGGTGATC 
CAGGAGCGGA TTGTCCACCT GCGCGGCGCA CTGAAGGAGC CCATCCACAT CCCCAAAACC 
ACCACAGCCA AACACAAAGG CAGACGGGAT GGAGAGGAAC TGGACAGCCA GGGGGATGCC 
AGCAGCCAAC CAGATACCAT CTCCATTGCC TCGCGAACAT CCCAGAACAC AATGGACAGC 
GATAAGCTCT CCGGTGGCTG TGAGTTGACA GTGGTGATCC ACGACTTCGT GGCCAGTAAT 
GGCAGCAGCG GCGAGCTGTC GGTGCGCCGA GGTCAGACTG TGGAAGTCCT GGAACGGCTC 
CACGACAAGC CCGACTGGTG CCTGGTGCGG ACCACGGACC GTTCCCCGGC TCAGGAGGGC 
ATCGTTCCCT GCTCCATGCT CTGCATCGCT CACTCGAGGT CTTCCATGGA GATGGAAGGG 
CTCTTTAACC ACAAAGACAC ATTATCTGTA TCCAGTAACG ACGCCCTCCA ACCAGGCTCC 
AGCCAAACCC TGGGTCCTCA CAGCTCCCCC GGCCCCAAAC GCCCAGGGAA CACACTGAGG 
AAGTGGCTGA CCAGCCCCGT GCGGAGGCTC AGCTCTGGCA AGGCCGACGG CCACGTGAAG 
AAACTGGCCC ACAAGCATAA GAAGAACCGC GAGGGCGCGA GGAAGAACAT GGATGCCATG 
CACGGTTCTC AGAAGGACTC TGATGACAGC GCCGCTACGC CCCAGGATGA AACGCTAGAG 
GAACGTATGC GTAACGAAGG GCTGAGCAGC GGCACCCTGT CCAAGTCTTC CTCTTCTGGC 
ATGCAGAGCT GTGGCGAGGA GGAAGGAGAG GAGGGAGCCG ACGCCGTCCC TCTACCGCCC 
CCTATGGCCA TCCAGCAGCA CAGCTTGCTG CAGCCTGACT CCCAGGACGA CAAGTCCGGA 
TCTCGTCTGT CCGCACGTCC AAGCAGCTCA GAGACGCCCA GCGCGGCCGA ACTAGTCAGC 
GCCATCGAGG AGCTGGTGAA GAGTAAGATG TCCCTGGAGG ATCGTCCCAG CTCTCTGTCA 
GTAGAACAAG GTGACAGCAG CTCTCCCTCC CTAAATCCCT CTGACAACTC CCTCCTCTCC 
TCCTCCTCAC CCATCGATGA GATGGACGAG CGCAAGGCCG ACTTCCTCAA GAGAAGACAT 
TACGTGCTAC TGGAGTTGGT GGAGACCGAG AGGGACTATG TCAGAGATCT GGGATCAGTG 
GTAGAGGGCT ACATGTGTAG GATGAAAGAA GAGGGTGTTC CTGATGACAT GAGGGGAAAA 
GACAAGATCG TCTTTGGAAA CATCCATCAG ATCTACGACT GGCACAAAGA TTTTTTCCTG 
GGGGAGCTTG AGAAGTGTTT AGAGGATCCC GACAGGCTGG CACCTTTATT CATTAAACAG 
GAGCGGAGGC TGCACATGTA CATCGTGTAC TGCCAGAACA AACCCAAGTC TGAGCACATC 
GTTTCAGAGT ACATCGACAC TTATTTTGAG GACCTAAAGC AGCGTTTGGG CCACAGGCTG 
CAGATCACTG ATCTATTAAT CAAGCCAGTC CAGCGTATAA TGAAGTACCA ATTACTGCTA 
AAAGATCTTT TGAAAATGTC CAAGAAGGCT GGCTTGGATA TGGCAGAGCT TGAGAAAGCT 
GTGGAGGTGA TGTGCGTGGT TCCCAAACGC TGCAACGACA TGATGAATGT TGGACGCCTT 
CAGGGTTTTG AAGGTAAAAT TGTGGCGCAG GGCAGATTAC TGCTCCAAGA TACCTTCATG 
GTGTCGGACC AGGATGGGGG ACTTCTGTCC AGGATGAAGG AGAGAAGGGT TTTCTTATTT 
GAACAGATAG TAATCTTTAG CGAACCTCTT GACAAAAAGA AGGGCTTCTC CACTCCAGGA 
TACCTCTTTA AGAACAGCAT CAAGGTGAGC TGGTTGGGTT TAGAAGAACA TTCAGAGGAC 
CCTTGCAAGT TCACGCTTAC CTCCAGATCA TCTAGTGGCA ACTTGGAGAG ATACACCCTC 
CATTCAATGA GTCCTGGGGT CAGTCAAGTC TGGGTTCACC AGATTAGCCA GATACTGGAG 
AACCAGCGCA ACTTCCTCAA TGCTTTGACA TCCCCCATTG AATACCAGAG AAACCATGTG 
GGTAGTGGAG TGCCAGGTGG TCTTCCCAGC AGCAGCAGCA GCAGTACTGG AGGCCTACCA 
GGAGGCAGCA GTTCCATTGG CTCCTCTAGC ATGGGAGTAG GAGGCGGCGG TGGCTCTGGG 
GGACCTGGGA ACAGCTCATC CTCCTCACTC TGCGGTCCCC GTTCCCGACC GTCCCGCATT 
CCACAGCCAT CGTCACGACT CCCACAACCC GTCCACCACC ACCATCCCCC GGGCCCTGAT 
GGGCCTGACA GATCAGCAGG TATGTGGCCG CCCTGCCACC CTCAGTCCCA CCCATCTAAC 
TCTTATTCAG ACAGGGCCAC AAATGGAGAA CCATTAGGCT CAGAGTTCCC CAAGATGAAA 
AGATTGGAGC TTCACGGAAA TAACAGCAAC AACAGCAACA AGTCCTCAGA CAGTTATGAA 
GACACTCAGA AGCACCCGGC AGCTGGTATA CCGCAGATGG CTGTTGCGCC TCTGAATTTG 
ACCCTAAAAA GCCCCCGAGT CGGGACAGTT TCTCCTCTGA TTTCACCTCA GTCTCCTGCA 
GGAGGAAAAG AAGCATTTAT CCCTTCAAGT CAAGCTCATA AAGGCAACTT GTTCTGGGCT 
GTGGTCCCTC CTATGCCTTC CTCTGTGTCG AGTCGGCCCA GTTCGCTCTC CTACCCCAGC 
GATAGTGTTG GTGGAGGGGA TTCTCTGGGA AGAGGCGGCC ACTGTACGCC CTCTCACCAT 
CGCCAGTCCA CTCACAGTAA GGACATAGAC CGCATGAGCA CCTGCTCCTC CACCAGCGAG 
CAGTCCATAC AGTCCATCCA GAGCAATGGG AGTGAAAACA GTAGCAGCAG CATTGTGTCC 
ACCATGTTGG TGACCCAGGA CTATGTGGCA GTGAAGGAGG ATGAGATCAG TGTGGTCCAG 
GGTGAGGTGG TCCAAATACT GGCCAGCAAC CAACAAAACA TGTTCCTGGT GTACCGGGCA 
GCCAACGAGC ACTCTCCTGC TGCTGAGGGC TGGATCCCTG GCTATGTATT AGGACACACC 
TCATCCTCTG TCACACCTGA GCTCCCTGAA GGAACCATCA AAAAATCACT ATCATGGCAC 
ACAGCACTCC GAATTCGCCG TAAATCTGAA AAAAGGGACA AGGAGGGCAG GAAGATCGAG 
AACGGGTACC GCAAATCTCA GGACAGCCTG GCCAACAAAG TCTCTGTTAA GCTTCTGAAT 
CCCAATTTCA TTCCTGACGC ACCTCCAGAA TTCCTCATCC CTGTCACCGA TGTAGCATGT 
GAGAGCGGCG ACACTGTGAC ACTCAGGTGC AAAATCTGTA GCCGGCCTCG CGCCACCGTT 
ACCTGGCGAG GACCTGACAA CGGCGTTCGG AGCAACAACG GACGCTACAG CACCACCTAC 
AGTGAGACAG AAGAAGCGAC TCTCCGTATT GTGATGGTGT CCGTAGAGGA CAGTGGCGTG 
TACACGTGTG TCGCCACAAA TGTTGCAGGC TCCGTCACGT CCTCCGCCAA CCTCACAGTC 
TCAGCTTCGC ATGATGGCAA CAGTGAGATC TGGAAGAGCA GCTTTGAGTT CAACTACACA 
GAGATCACAG AACTAGGAAG GGGCCGTTTC TCAGTGACCA AACGGTGCGA CCAGAGAGGG 
AGCAAGCGCA CGGTTGCGGC CAAATACGTT AGCAAGAAGC TGCTGCGGCG AGAGCAGGTG 
CTGCAGGAGA TCAGACTCCT GCAGACTCTG GACCATCCCA ACCTGGTTAA GCTTCTGGAC 
ACCTATGAAA TGGCCAACAG CTATGTTCTC GTGCTAGAAA TGGCGGACCA GGGACGTTTC 
TTGGATTACG TAGTGAGCTG GGGCAACCTC ACAGAGGAGA AGGTGGCACT GTACCTCAGA 
GAAATTCTTG AAGCTCTTCA CTACCTGCAC GGCTGGAGGA TAGCGCACCT CGACCTCAAA 
CCTGAGAACA TTGTGGTGGA ACATGCATCC TCGCAGCCGG TGATCAAGCT GACAGACTTT 
GGTGATGCCG TGCAACTGAA TCCAGCCTCC TCGTACATCC ATCCTCTGCT GGGGAGCCCA 
GAGTTCTCTG CTCCTGAGCT GGTCCTCGGG CAGCCCTCCT CGCTGACGTC GGACATCTGG 
AGTTTAGGGG TGGTGACATA CATTGTACTC AGTGGCGCTT CCCCCTTCCT GGATGAGAGT 
TTGGAGGAGA CGTGTCTGAA CATCTGTCGC CTGGACTTCA GCTTCCCGGA GGACTACTTC 
CAGGGAGTGA GCCTGGCTGC CAAGGACTTT GTCTGCCTGC TGCTCCAGAG CGAGCCGGAG 
CGGCGGCCCA CTGCGTTAGC CTGCCTGCAG GAGCAGTGGC TCCAGCCCCG AGGTGTGATC 
AATACTGGAT TTGTAGGCTC GAAACATGCA CACGCGTCGC CCAACCATCA CACTTTACAT 
CTGGACACAT CCAGACTCAT TTCTTTCATT GAGAGGCGGA AACACCAGAA CGACATTCGG 
CCCATAGACA CGGTCAGGGC CTTCCTCCAC AGCCGCCTGC TTAACAACAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029695077.1
CDS	203..9616
Translation	MSAGEGLDEAAKDAADIAAFFKSDCALPKCESKLDGPGQTSGFHRNEDMKAIDVLPILKEKVAFLSGGRDRRGGPVLTFPARSNHDRIRPEDLRRLIAYLATIPSEEVARHGFTVIVDMRGSKWDSIKPLLKILQESFPSCIHVALIIKPDNFWQKQRTNFGSSKFEFETVMVSLEGLSKIVDPSQLTADFEGSLEYNHDDWIEVRLSFETFASDAARALARLEELQDTLSQRDLPRDLEGARRLMEEHAALKKRATKASVEELDTQGRRLLQRLQAQATGGGSVNESGYINRLGGASGDPNDHHHGCHTHADAHNLITKVSSLLEKLHGTRQNLQQLWHMRKLKLDQCFQLRLFEQDAEKMFDWIMHNKGLFLTSYTEIGGNHQHAVELQTQHNHFAMNCMNVYVNINRIMSVGNRLLESGHYASQQIQQISGQLEQEWKAFAAALDERSTLLEMSASFHQKADQYMGKVEPWCKACGEGELPSELQDLEDTIHHHQGLYEHITTAYSEVSQDGKSLLDKLQRPLTPGSADSLMASANYSKAVHHVLDIIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKVLLDMSVAFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQATVNVIKEGEDLIQQLRDSAISSNKTPHNSSMAHIESVLQQLDEAQGQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNEELSQQMSDFDTEDLTLAEQRLQHHADKALTMNNLTYDVIHQGQELLQYVTEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRRHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQKEHEQFQHAIEKTHQSALQVQQKAETLLQANHYDMDMIRDCAEKVADHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSESDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMLSHVKAPETQVKNILNELLQRENRVLHFWTMRKRRLDQCQQYVVFERSAKQALEWIHDTGEFYLSTHTSTGSSIHHTQELLKEHEDFQITAKQTKERVKLLIQLADGFCDKGHAHALEIKKWVSSVDKRYRDFSLRMDKYRTCLETALGISSDSNKASKELQLDIIPASAPGSEVKLRDAAHDLNEEKRKSARRKEFIMAELIQTEKAYVRDLRECLDTYLWEMTSGVEEIPAGIVNKEHIIFGNMQDLYEFHHNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVSYCKNKPDSTQLILEHAGAYFDEIQQRHRLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKKANDAMHLSMLEGFDGNIDSQGELILQESFQVWDPKTLIRKGRDRHLFLFEMSLVLSKEVKDSNGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERIVHLRGALKEPIHIPKTTTAKHKGRRDGEELDSQGDASSQPDTISIASRTSQNTMDSDKLSGGCELTVVIHDFVASNGSSGELSVRRGQTVEVLERLHDKPDWCLVRTTDRSPAQEGIVPCSMLCIAHSRSSMEMEGLFNHKDTLSVSSNDALQPGSSQTLGPHSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKNREGARKNMDAMHGSQKDSDDSAATPQDETLEERMRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKSGSRLSARPSSSETPSAAELVSAIEELVKSKMSLEDRPSSLSVEQGDSSSPSLNPSDNSLLSSSSPIDEMDERKADFLKRRHYVLLELVETERDYVRDLGSVVEGYMCRMKEEGVPDDMRGKDKIVFGNIHQIYDWHKDFFLGELEKCLEDPDRLAPLFIKQERRLHMYIVYCQNKPKSEHIVSEYIDTYFEDLKQRLGHRLQITDLLIKPVQRIMKYQLLLKDLLKMSKKAGLDMAELEKAVEVMCVVPKRCNDMMNVGRLQGFEGKIVAQGRLLLQDTFMVSDQDGGLLSRMKERRVFLFEQIVIFSEPLDKKKGFSTPGYLFKNSIKVSWLGLEEHSEDPCKFTLTSRSSSGNLERYTLHSMSPGVSQVWVHQISQILENQRNFLNALTSPIEYQRNHVGSGVPGGLPSSSSSSTGGLPGGSSSIGSSSMGVGGGGGSGGPGNSSSSSLCGPRSRPSRIPQPSSRLPQPVHHHHPPGPDGPDRSAGMWPPCHPQSHPSNSYSDRATNGEPLGSEFPKMKRLELHGNNSNNSNKSSDSYEDTQKHPAAGIPQMAVAPLNLTLKSPRVGTVSPLISPQSPAGGKEAFIPSSQAHKGNLFWAVVPPMPSSVSSRPSSLSYPSDSVGGGDSLGRGGHCTPSHHRQSTHSKDIDRMSTCSSTSEQSIQSIQSNGSENSSSSIVSTMLVTQDYVAVKEDEISVVQGEVVQILASNQQNMFLVYRAANEHSPAAEGWIPGYVLGHTSSSVTPELPEGTIKKSLSWHTALRIRRKSEKRDKEGRKIENGYRKSQDSLANKVSVKLLNPNFIPDAPPEFLIPVTDVACESGDTVTLRCKICSRPRATVTWRGPDNGVRSNNGRYSTTYSETEEATLRIVMVSVEDSGVYTCVATNVAGSVTSSANLTVSASHDGNSEIWKSSFEFNYTEITELGRGRFSVTKRCDQRGSKRTVAAKYVSKKLLRREQVLQEIRLLQTLDHPNLVKLLDTYEMANSYVLVLEMADQGRFLDYVVSWGNLTEEKVALYLREILEALHYLHGWRIAHLDLKPENIVVEHASSQPVIKLTDFGDAVQLNPASSYIHPLLGSPEFSAPELVLGQPSSLTSDIWSLGVVTYIVLSGASPFLDESLEETCLNICRLDFSFPEDYFQGVSLAAKDFVCLLLQSEPERRPTALACLQEQWLQPRGVINTGFVGSKHAHASPNHHTLHLDTSRLISFIERRKHQNDIRPIDTVRAFLHSRLLNNI

Target ORF information:

RefSeq Version	XM_029839217.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes triple functional domain protein-like (LOC101071578), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029839217.1

ORF Insert Sequence:

ATGAGCGCAG GAGAGGGATT AGATGAGGCT GCGAAGGACG CGGCAGACAT AGCGGCGTTT 
TTCAAATCCG ATTGCGCTCT CCCAAAATGT GAATCCAAAC TGGACGGGCC AGGTCAAACC 
TCAGGGTTTC ACCGCAATGA GGATATGAAG GCCATTGACG TACTTCCTAT CCTCAAGGAG 
AAGGTCGCCT TTCTCTCAGG TGGCAGAGAC AGACGCGGAG GTCCCGTTCT CACATTTCCT 
GCTCGGAGCA ACCATGACAG AATACGACCT GAAGACCTCC GCAGGCTCAT AGCTTACCTG 
GCTACTATTC CCAGCGAGGA AGTGGCGAGG CATGGCTTCA CGGTCATTGT GGACATGCGT 
GGCTCTAAAT GGGACAGCAT CAAGCCTTTG CTGAAGATCC TTCAGGAGTC GTTCCCATCT 
TGCATCCACG TTGCCCTCAT CATCAAGCCC GACAACTTCT GGCAGAAGCA GAGGACAAAC 
TTCGGCAGCT CCAAATTTGA ATTCGAGACG GTGATGGTCT CTTTAGAGGG TTTATCCAAA 
ATCGTGGACC CATCACAGCT GACAGCTGAC TTTGAGGGCA GCCTGGAATA CAACCATGAT 
GACTGGATCG AGGTGCGGCT GTCCTTTGAG ACGTTTGCCA GCGATGCGGC ACGTGCGCTG 
GCACGCCTGG AGGAGCTCCA GGACACGTTG TCCCAGCGTG ATCTCCCGCG GGACCTGGAA 
GGAGCTCGGC GGCTCATGGA GGAACACGCG GCGCTCAAGA AAAGGGCCAC GAAAGCATCC 
GTGGAGGAGC TGGACACGCA GGGGCGACGG CTGCTGCAGA GGCTGCAGGC ACAGGCTACG 
GGAGGAGGCA GCGTTAATGA AAGCGGGTAC ATTAACCGCC TGGGCGGAGC TAGCGGAGAC 
CCCAACGACC ACCATCACGG GTGCCACACG CACGCAGACG CACACAACCT CATCACCAAA 
GTGAGCAGCT TGTTGGAAAA GCTTCACGGG ACTCGGCAAA ATCTGCAGCA GCTGTGGCAC 
ATGAGGAAGC TGAAGCTGGA CCAGTGTTTC CAGCTGCGTC TTTTTGAACA GGACGCAGAG 
AAGATGTTCG ACTGGATCAT GCACAACAAG GGGCTTTTTC TCACGAGTTA CACGGAGATC 
GGGGGGAACC ACCAGCACGC TGTCGAGCTG CAGACCCAAC ACAACCACTT TGCCATGAAC 
TGCATGAATG TGTACGTGAA CATCAACCGG ATCATGTCAG TCGGGAACCG GCTCCTGGAG 
TCGGGCCATT ACGCCTCGCA GCAGATCCAG CAGATCTCCG GGCAGCTGGA GCAGGAGTGG 
AAGGCCTTCG CTGCGGCGCT GGATGAACGC AGCACGCTGC TGGAGATGTC CGCCAGCTTC 
CACCAGAAAG CGGACCAGTA CATGGGGAAA GTGGAACCGT GGTGTAAAGC CTGTGGTGAA 
GGAGAGCTGC CCTCTGAACT CCAGGATCTG GAAGACACAA TCCACCATCA TCAGGGGCTT 
TATGAGCACA TCACCACTGC TTACTCTGAG GTAAGCCAGG ATGGGAAGTC ACTCTTGGAC 
AAGCTGCAGC GCCCTTTGAC CCCAGGGAGC GCTGATTCCT TGATGGCCTC TGCCAACTAC 
TCCAAGGCCG TGCATCACGT CTTAGACATC ATCCACGAGG TGCTGCACCA CCAGAGGCAG 
CTGGAAAACA TCTGGCAGCA TCGCAAAGTC CGCCTGCACC AACGGCTGCA ACTGTGTGTT 
TTTCAGCAGG ATGTGCAGCA GGTACTGGAC TGGATAGAAA ACCACGGAGA AGCCTTCCTA 
AGCAAACACA CTGGTGTTGG CAAGTCTCTG CACAGAGCCA GAGCTCTGCA GAAGAGGCAT 
GAGGATTTTG AAGAGGTTGC ACAGAACACC TACACTAATG CTGACAAACT GCTGGAAGCC 
GCAGAGCAGC TGGCTCAGAC CGGCGAATGT GATCCAGAGG AAATTTACCA GGCTGCCCAC 
CAGCTGGAAG ATCGCATCCA GGACTTCGTA CGACGTGTCG AGCAGAGAAA AGTCCTGTTG 
GACATGTCTG TCGCCTTCCA CACGCATGTC AAAGAGCTGT GGACGTGGCT GGAGGAGTTG 
CAGAAGGAAT TGCTAGATGA CGTATACGCA GAATCCGTGG AGGCTGTTCA GGACCTGATC 
AAACGCTTCG GCCAGCAGCA GCAGACCACC CTGCAGGCTA CTGTCAATGT CATCAAAGAG 
GGAGAGGACC TTATCCAACA GCTCAGAGAC TCGGCTATCT CAAGCAACAA AACTCCCCAT 
AACAGCTCCA TGGCCCACAT CGAGAGTGTG CTGCAGCAGC TGGACGAGGC TCAGGGCCAG 
ATGGAGGAAC TGTTTCAGGA AAGGAAGATC AAACTGGAGC TCTTTCTGCA GCTGCGCATC 
TTCGAGAGGG ACGCCATCGA TATCATCTCT GACCTGGAGT CATGGAACGA GGAGCTCTCC 
CAGCAGATGA GTGACTTCGA CACCGAGGAT CTGACGCTGG CGGAGCAGAG GCTGCAGCAC 
CACGCAGACA AGGCGCTCAC CATGAACAAC CTGACCTACG ACGTCATCCA CCAGGGCCAA 
GAGCTGCTGC AGTACGTGAC CGAGGTCCAG GCGTCTGGTG TGGAGCTGCT GTGCGACAGA 
GATGTGGACA TGGCCACACG GGTCCAGGAC CTGCTGGAGT TCCTCCACGA GAAGCAGCAG 
GAGCTGGACC TGGCGGCGGA GCAGCACAGG AGACACTTGG AACAGTGTGT CCAACTGAGA 
CACCTCCAGG CAGAAGTCAA ACAGGTGCTG GGATGGATCC GGAATGGCGA GTCCATGCTG 
AACGCTGGTC TGATCACAGC CAGCTCGTTA CAGGAAGCTG AACAGCTGCA GAAGGAACAT 
GAACAATTTC AGCATGCAAT AGAGAAAACT CATCAGAGCG CGCTGCAGGT GCAGCAGAAG 
GCCGAGACCT TACTCCAGGC AAACCACTAT GACATGGACA TGATCAGGGA CTGTGCAGAA 
AAGGTGGCAG ACCACTGGCA GCAGCTCATG CTGAAAATGG AAGACCGACT CAAGCTGGTC 
AACGCTTCTG TGGCCTTCTA CAAAACCTCC GAACAGGTTT GCAGTGTGTT GGAAAGCCTG 
GAGCAGGAGT ACAAGAGAGA AGAGGACTGG TGTGGTGGCG CTGATAAACT AGGCCCCAAC 
AGCGAGTCCG ACCACGTCAC TCCCATGATC AGCAAACATC TGGAGCAGAA AGAGGCCTTT 
CTCAAGGCGT GCACGCTAGC CCGGCGGAAT GCTGACGTCT TCTTGAAGTA CCTGCACAGA 
AACAGTGTCA ATATGCCCGG CATGCTGTCC CACGTCAAAG CTCCAGAGAC TCAGGTTAAG 
AATATCTTGA ACGAGCTGCT CCAGAGAGAG AACCGAGTCC TTCACTTCTG GACAATGAGG 
AAGAGGAGGC TGGACCAGTG TCAGCAATAT GTCGTATTTG AACGCAGCGC CAAACAGGCT 
CTGGAGTGGA TCCATGACAC TGGGGAGTTT TACTTGTCCA CACACACATC CACTGGCTCC 
AGCATTCATC ACACACAAGA GCTGCTGAAG GAGCATGAGG ACTTCCAGAT CACAGCAAAG 
CAAACCAAAG AGCGAGTAAA GCTGTTGATC CAGCTGGCAG ATGGGTTTTG TGACAAGGGG 
CACGCCCATG CACTGGAAAT AAAGAAATGG GTGTCTTCTG TGGACAAGCG CTACCGGGAC 
TTCTCTCTCC GCATGGACAA ATACCGTACC TGCCTGGAAA CTGCGCTCGG CATATCATCC 
GACTCCAACA AGGCTAGCAA AGAGCTCCAG CTGGATATAA TCCCAGCCAG CGCTCCGGGC 
TCAGAGGTCA AACTCCGGGA TGCTGCCCAT GACCTCAATG AGGAGAAGAG GAAGTCGGCA 
CGGAGGAAGG AATTCATAAT GGCGGAGCTG ATTCAAACAG AAAAAGCCTA CGTCAGGGAC 
CTAAGGGAGT GTTTGGATAC GTACCTGTGG GAGATGACCA GTGGTGTGGA GGAGATTCCC 
GCCGGAATTG TCAACAAAGA GCACATCATC TTTGGGAACA TGCAGGACCT CTACGAGTTC 
CACCACAACA TCTTCCTGAA GGAGTTGGAG AAGTATGAGC AACTTCCAGA AGATGTCGGC 
CATTGTTTTG TCACTTGGGC TGATAAGTTC CAGATGTACG TCAGTTACTG TAAGAACAAA 
CCCGACTCCA CGCAGCTCAT CCTCGAACAT GCCGGAGCAT ACTTCGATGA AATCCAGCAA 
AGACATCGCC TTGCTAACTC CATCTCCTCT TACCTGATCA AACCAGTGCA GAGAATCACA 
AAGTACCAGC TGCTTCTCAA GGAGCTGCTG ACATGCTGCG AGGAGGGAAA AGGTGAGATC 
AAGGACGGAC TGGAAGTGAT GCTCAGCGTC CCTAAGAAAG CCAACGACGC CATGCACCTG 
TCTATGCTGG AGGGGTTTGA TGGAAACATT GACTCCCAGG GAGAGCTGAT ACTTCAGGAA 
TCCTTCCAGG TCTGGGATCC TAAGACTCTG ATCCGAAAGG GTCGGGACCG GCACCTCTTC 
CTGTTTGAGA TGTCTCTGGT GTTAAGCAAA GAAGTCAAAG ATTCCAACGG GCGCAGCAAA 
TACCTGTACA AGAGCAAGCT GTTTACATCA GAACTTGGAG TTACGGAACA TGTGGAGGGA 
GATCCCTGTA AGTTCGCCCT GTGGGTGGGC AGGACCCCAA CGTCTGACAA CAAGATCGTC 
CTCAAGGCAT CCAGTATCGA GAACAAACAG GACTGGATCA AACACATCCG GGAGGTGATC 
CAGGAGCGGA TTGTCCACCT GCGCGGCGCA CTGAAGGAGC CCATCCACAT CCCCAAAACC 
ACCACAGCCA AACACAAAGG CAGACGGGAT GGAGAGGAAC TGGACAGCCA GGGGGATGCC 
AGCAGCCAAC CAGATACCAT CTCCATTGCC TCGCGAACAT CCCAGAACAC AATGGACAGC 
GATAAGCTCT CCGGTGGCTG TGAGTTGACA GTGGTGATCC ACGACTTCGT GGCCAGTAAT 
GGCAGCAGCG GCGAGCTGTC GGTGCGCCGA GGTCAGACTG TGGAAGTCCT GGAACGGCTC 
CACGACAAGC CCGACTGGTG CCTGGTGCGG ACCACGGACC GTTCCCCGGC TCAGGAGGGC 
ATCGTTCCCT GCTCCATGCT CTGCATCGCT CACTCGAGGT CTTCCATGGA GATGGAAGGG 
CTCTTTAACC ACAAAGACAC ATTATCTGTA TCCAGTAACG ACGCCCTCCA ACCAGGCTCC 
AGCCAAACCC TGGGTCCTCA CAGCTCCCCC GGCCCCAAAC GCCCAGGGAA CACACTGAGG 
AAGTGGCTGA CCAGCCCCGT GCGGAGGCTC AGCTCTGGCA AGGCCGACGG CCACGTGAAG 
AAACTGGCCC ACAAGCATAA GAAGAACCGC GAGGGCGCGA GGAAGAACAT GGATGCCATG 
CACGGTTCTC AGAAGGACTC TGATGACAGC GCCGCTACGC CCCAGGATGA AACGCTAGAG 
GAACGTATGC GTAACGAAGG GCTGAGCAGC GGCACCCTGT CCAAGTCTTC CTCTTCTGGC 
ATGCAGAGCT GTGGCGAGGA GGAAGGAGAG GAGGGAGCCG ACGCCGTCCC TCTACCGCCC 
CCTATGGCCA TCCAGCAGCA CAGCTTGCTG CAGCCTGACT CCCAGGACGA CAAGTCCGGA 
TCTCGTCTGT CCGCACGTCC AAGCAGCTCA GAGACGCCCA GCGCGGCCGA ACTAGTCAGC 
GCCATCGAGG AGCTGGTGAA GAGTAAGATG TCCCTGGAGG ATCGTCCCAG CTCTCTGTCA 
GTAGAACAAG GTGACAGCAG CTCTCCCTCC CTAAATCCCT CTGACAACTC CCTCCTCTCC 
TCCTCCTCAC CCATCGATGA GATGGACGAG CGCAAGGCCG ACTTCCTCAA GAGAAGACAT 
TACGTGCTAC TGGAGTTGGT GGAGACCGAG AGGGACTATG TCAGAGATCT GGGATCAGTG 
GTAGAGGGCT ACATGTGTAG GATGAAAGAA GAGGGTGTTC CTGATGACAT GAGGGGAAAA 
GACAAGATCG TCTTTGGAAA CATCCATCAG ATCTACGACT GGCACAAAGA TTTTTTCCTG 
GGGGAGCTTG AGAAGTGTTT AGAGGATCCC GACAGGCTGG CACCTTTATT CATTAAACAG 
GAGCGGAGGC TGCACATGTA CATCGTGTAC TGCCAGAACA AACCCAAGTC TGAGCACATC 
GTTTCAGAGT ACATCGACAC TTATTTTGAG GACCTAAAGC AGCGTTTGGG CCACAGGCTG 
CAGATCACTG ATCTATTAAT CAAGCCAGTC CAGCGTATAA TGAAGTACCA ATTACTGCTA 
AAAGATCTTT TGAAAATGTC CAAGAAGGCT GGCTTGGATA TGGCAGAGCT TGAGAAAGCT 
GTGGAGGTGA TGTGCGTGGT TCCCAAACGC TGCAACGACA TGATGAATGT TGGACGCCTT 
CAGGGTTTTG AAGGTAAAAT TGTGGCGCAG GGCAGATTAC TGCTCCAAGA TACCTTCATG 
GTGTCGGACC AGGATGGGGG ACTTCTGTCC AGGATGAAGG AGAGAAGGGT TTTCTTATTT 
GAACAGATAG TAATCTTTAG CGAACCTCTT GACAAAAAGA AGGGCTTCTC CACTCCAGGA 
TACCTCTTTA AGAACAGCAT CAAGGTGAGC TGGTTGGGTT TAGAAGAACA TTCAGAGGAC 
CCTTGCAAGT TCACGCTTAC CTCCAGATCA TCTAGTGGCA ACTTGGAGAG ATACACCCTC 
CATTCAATGA GTCCTGGGGT CAGTCAAGTC TGGGTTCACC AGATTAGCCA GATACTGGAG 
AACCAGCGCA ACTTCCTCAA TGCTTTGACA TCCCCCATTG AATACCAGAG AAACCATGTG 
GGTAGTGGAG TGCCAGGTGG TCTTCCCAGC AGCAGCAGCA GCAGTACTGG AGGCCTACCA 
GGAGGCAGCA GTTCCATTGG CTCCTCTAGC ATGGGAGTAG GAGGCGGCGG TGGCTCTGGG 
GGACCTGGGA ACAGCTCATC CTCCTCACTC TGCGGTCCCC GTTCCCGACC GTCCCGCATT 
CCACAGCCAT CGTCACGACT CCCACAACCC GTCCACCACC ACCATCCCCC GGGCCCTGAT 
GGGCCTGACA GATCAGCAGG TATGTGGCCG CCCTGCCACC CTCAGTCCCA CCCATCTAAC 
TCTTATTCAG ACAGGGCCAC AAATGGAGAA CCATTAGGCT CAGAGTTCCC CAAGATGAAA 
AGATTGGAGC TTCACGGAAA TAACAGCAAC AACAGCAACA AGTCCTCAGA CAGTTATGAA 
GACACTCAGA AGCACCCGGC AGCTGGTATA CCGCAGATGG CTGTTGCGCC TCTGAATTTG 
ACCCTAAAAA GCCCCCGAGT CGGGACAGTT TCTCCTCTGA TTTCACCTCA GTCTCCTGCA 
GGAGGAAAAG AAGCATTTAT CCCTTCAAGT CAAGCTCATA AAGGCAACTT GTTCTGGGCT 
GTGGTCCCTC CTATGCCTTC CTCTGTGTCG AGTCGGCCCA GTTCGCTCTC CTACCCCAGC 
GATAGTGTTG GTGGAGGGGA TTCTCTGGGA AGAGGCGGCC ACTGTACGCC CTCTCACCAT 
CGCCAGTCCA CTCACAGTAA GGACATAGAC CGCATGAGCA CCTGCTCCTC CACCAGCGAG 
CAGTCCATAC AGTCCATCCA GAGCAATGGG AGTGAAAACA GTAGCAGCAG CATTGTGTCC 
ACCATGTTGG TGACCCAGGA CTATGTGGCA GTGAAGGAGG ATGAGATCAG TGTGGTCCAG 
GGTGAGGTGG TCCAAATACT GGCCAGCAAC CAACAAAACA TGTTCCTGGT GTACCGGGCA 
GCCAACGAGC ACTCTCCTGC TGCTGAGGGC TGGATCCCTG GCTATGTATT AGGACACACC 
TCATCCTCTG TCACACCTGA GCTCCCTGAA GGAACCATCA AAAAATCACT ATCATGGCAC 
ACAGCACTCC GAATTCGCCG TAAATCTGAA AAAAGGGACA AGGAGGGCAG GAAGATCGAG 
AACGGGTACC GCAAATCTCA GGACAGCCTG GCCAACAAAG TCTCTGTTAA GCTTCTGAAT 
CCCAATTTCA TTCCTGACGC ACCTCCAGAA TTCCTCATCC CTGTCACCGA TGTAGCATGT 
GAGAGCGGCG ACACTGTGAC ACTCAGGTGC AAAATCTGTA GCCGGCCTCG CGCCACCGTT 
ACCTGGCGAG GACCTGACAA CGGCGTTCGG AGCAACAACG GACGCTACAG CACCACCTAC 
AGTGAGACAG AAGAAGCGAC TCTCCGTATT GTGATGGTGT CCGTAGAGGA CAGTGGCGTG 
TACACGTGTG TCGCCACAAA TGTTGCAGGC TCCGTCACGT CCTCCGCCAA CCTCACAGTC 
TCAGCTTCGC ATGATGGCAA CAGTGAGATC TGGAAGAGCA GCTTTGAGTT CAACTACACA 
GAGATCACAG AACTAGGAAG GGGCCGTTTC TCAGTGACCA AACGGTGCGA CCAGAGAGGG 
AGCAAGCGCA CGGTTGCGGC CAAATACGTT AGCAAGAAGC TGCTGCGGCG AGAGCAGGTG 
CTGCAGGAGA TCAGACTCCT GCAGACTCTG GACCATCCCA ACCTGGTTAA GCTTCTGGAC 
ACCTATGAAA TGGCCAACAG CTATGTTCTC GTGCTAGAAA TGGCGGACCA GGGACGTTTC 
TTGGATTACG TAGTGAGCTG GGGCAACCTC ACAGAGGAGA AGGTGGCACT GTACCTCAGA 
GAAATTCTTG AAGCTCTTCA CTACCTGCAC GGCTGGAGGA TAGCGCACCT CGACCTCAAA 
CCTGAGAACA TTGTGGTGGA ACATGCATCC TCGCAGCCGG TGATCAAGCT GACAGACTTT 
GGTGATGCCG TGCAACTGAA TCCAGCCTCC TCGTACATCC ATCCTCTGCT GGGGAGCCCA 
GAGTTCTCTG CTCCTGAGCT GGTCCTCGGG CAGCCCTCCT CGCTGACGTC GGACATCTGG 
AGTTTAGGGG TGGTGACATA CATTGTACTC AGTGGCGCTT CCCCCTTCCT GGATGAGAGT 
TTGGAGGAGA CGTGTCTGAA CATCTGTCGC CTGGACTTCA GCTTCCCGGA GGACTACTTC 
CAGGGAGTGA GCCTGGCTGC CAAGGACTTT GTCTGCCTGC TGCTCCAGAG CGAGCCGGAG 
CGGCGGCCCA CTGCGTTAGC CTGCCTGCAG GAGCAGTGGC TCCAGCCCCG AGGTGTGATC 
AATACTGGAT TTGTAGGCTC GAAACATGCA CACGCGTCGC CCAACCATCA CACTTTACAT 
CTGGACACAT CCAGACTCAT TTCTTTCATT GAGAGGCGGA AACACCAGAA CGACATTCGG 
CCCATAGACA CGGTCAGGGC CTTCCTCCAC AGCCGCCTGC TTAACAACAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101071578 ( XM_029839219.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029695079.1 Takifugu rubripes triple functional domain protein-like (LOC101071578), transcript variant X3, mRNA.

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CloneID	OTo69929
Clone ID Related Accession (Same CDS sequence)	XM_029839219.1
Accession Version	XM_029839219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	triple functional domain protein-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042291.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGTGT TGGATAACCA CAGCTGGCAG TATGCCCAAG CTGGGAAAAT GCTCATCAGA 
TTATCAGGGT TTCACCGCAA TGAGGATATG AAGGCCATTG ACGTACTTCC TATCCTCAAG 
GAGAAGGTCG CCTTTCTCTC AGGTGGCAGA GACAGACGCG GAGGTCCCGT TCTCACATTT 
CCTGCTCGGA GCAACCATGA CAGAATACGA CCTGAAGACC TCCGCAGGCT CATAGCTTAC 
CTGGCTACTA TTCCCAGCGA GGAAGTGGCG AGGCATGGCT TCACGGTCAT TGTGGACATG 
CGTGGCTCTA AATGGGACAG CATCAAGCCT TTGCTGAAGA TCCTTCAGGA GTCGTTCCCA 
TCTTGCATCC ACGTTGCCCT CATCATCAAG CCCGACAACT TCTGGCAGAA GCAGAGGACA 
AACTTCGGCA GCTCCAAATT TGAATTCGAG ACGGTGATGG TCTCTTTAGA GGGTTTATCC 
AAAATCGTGG ACCCATCACA GCTGACAGCT GACTTTGAGG GCAGCCTGGA ATACAACCAT 
GATGACTGGA TCGAGGTGCG GCTGTCCTTT GAGACGTTTG CCAGCGATGC GGCACGTGCG 
CTGGCACGCC TGGAGGAGCT CCAGGACACG TTGTCCCAGC GTGATCTCCC GCGGGACCTG 
GAAGGAGCTC GGCGGCTCAT GGAGGAACAC GCGGCGCTCA AGAAAAGGGC CACGAAAGCA 
TCCGTGGAGG AGCTGGACAC GCAGGGGCGA CGGCTGCTGC AGAGGCTGCA GGCACAGGCT 
ACGGGAGGAG GCAGCGTTAA TGAAAGCGGG TACATTAACC GCCTGGGCGG AGCTAGCGGA 
GACCCCAACG ACCACCATCA CGGGTGCCAC ACGCACGCAG ACGCACACAA CCTCATCACC 
AAAGTGAGCA GCTTGTTGGA AAAGCTTCAC GGGACTCGGC AAAATCTGCA GCAGCTGTGG 
CACATGAGGA AGCTGAAGCT GGACCAGTGT TTCCAGCTGC GTCTTTTTGA ACAGGACGCA 
GAGAAGATGT TCGACTGGAT CATGCACAAC AAGGGGCTTT TTCTCACGAG TTACACGGAG 
ATCGGGGGGA ACCACCAGCA CGCTGTCGAG CTGCAGACCC AACACAACCA CTTTGCCATG 
AACTGCATGA ATGTGTACGT GAACATCAAC CGGATCATGT CAGTCGGGAA CCGGCTCCTG 
GAGTCGGGCC ATTACGCCTC GCAGCAGATC CAGCAGATCT CCGGGCAGCT GGAGCAGGAG 
TGGAAGGCCT TCGCTGCGGC GCTGGATGAA CGCAGCACGC TGCTGGAGAT GTCCGCCAGC 
TTCCACCAGA AAGCGGACCA GTACATGGGG AAAGTGGAAC CGTGGTGTAA AGCCTGTGGT 
GAAGGAGAGC TGCCCTCTGA ACTCCAGGAT CTGGAAGACA CAATCCACCA TCATCAGGGG 
CTTTATGAGC ACATCACCAC TGCTTACTCT GAGGTAAGCC AGGATGGGAA GTCACTCTTG 
GACAAGCTGC AGCGCCCTTT GACCCCAGGG AGCGCTGATT CCTTGATGGC CTCTGCCAAC 
TACTCCAAGG CCGTGCATCA CGTCTTAGAC ATCATCCACG AGGTGCTGCA CCACCAGAGG 
CAGCTGGAAA ACATCTGGCA GCATCGCAAA GTCCGCCTGC ACCAACGGCT GCAACTGTGT 
GTTTTTCAGC AGGATGTGCA GCAGGTACTG GACTGGATAG AAAACCACGG AGAAGCCTTC 
CTAAGCAAAC ACACTGGTGT TGGCAAGTCT CTGCACAGAG CCAGAGCTCT GCAGAAGAGG 
CATGAGGATT TTGAAGAGGT TGCACAGAAC ACCTACACTA ATGCTGACAA ACTGCTGGAA 
GCCGCAGAGC AGCTGGCTCA GACCGGCGAA TGTGATCCAG AGGAAATTTA CCAGGCTGCC 
CACCAGCTGG AAGATCGCAT CCAGGACTTC GTACGACGTG TCGAGCAGAG AAAAGTCCTG 
TTGGACATGT CTGTCGCCTT CCACACGCAT GTCAAAGAGC TGTGGACGTG GCTGGAGGAG 
TTGCAGAAGG AATTGCTAGA TGACGTATAC GCAGAATCCG TGGAGGCTGT TCAGGACCTG 
ATCAAACGCT TCGGCCAGCA GCAGCAGACC ACCCTGCAGG CTACTGTCAA TGTCATCAAA 
GAGGGAGAGG ACCTTATCCA ACAGCTCAGA GACTCGGCTA TCTCAAGCAA CAAAACTCCC 
CATAACAGCT CCATGGCCCA CATCGAGAGT GTGCTGCAGC AGCTGGACGA GGCTCAGGGC 
CAGATGGAGG AACTGTTTCA GGAAAGGAAG ATCAAACTGG AGCTCTTTCT GCAGCTGCGC 
ATCTTCGAGA GGGACGCCAT CGATATCATC TCTGACCTGG AGTCATGGAA CGAGGAGCTC 
TCCCAGCAGA TGAGTGACTT CGACACCGAG GATCTGACGC TGGCGGAGCA GAGGCTGCAG 
CACCACGCAG ACAAGGCGCT CACCATGAAC AACCTGACCT ACGACGTCAT CCACCAGGGC 
CAAGAGCTGC TGCAGTACGT GACCGAGGTC CAGGCGTCTG GTGTGGAGCT GCTGTGCGAC 
AGAGATGTGG ACATGGCCAC ACGGGTCCAG GACCTGCTGG AGTTCCTCCA CGAGAAGCAG 
CAGGAGCTGG ACCTGGCGGC GGAGCAGCAC AGGAGACACT TGGAACAGTG TGTCCAACTG 
AGACACCTCC AGGCAGAAGT CAAACAGGTG CTGGGATGGA TCCGGAATGG CGAGTCCATG 
CTGAACGCTG GTCTGATCAC AGCCAGCTCG TTACAGGAAG CTGAACAGCT GCAGAAGGAA 
CATGAACAAT TTCAGCATGC AATAGAGAAA ACTCATCAGA GCGCGCTGCA GGTGCAGCAG 
AAGGCCGAGA CCTTACTCCA GGCAAACCAC TATGACATGG ACATGATCAG GGACTGTGCA 
GAAAAGGTGG CAGACCACTG GCAGCAGCTC ATGCTGAAAA TGGAAGACCG ACTCAAGCTG 
GTCAACGCTT CTGTGGCCTT CTACAAAACC TCCGAACAGG TTTGCAGTGT GTTGGAAAGC 
CTGGAGCAGG AGTACAAGAG AGAAGAGGAC TGGTGTGGTG GCGCTGATAA ACTAGGCCCC 
AACAGCGAGT CCGACCACGT CACTCCCATG ATCAGCAAAC ATCTGGAGCA GAAAGAGGCC 
TTTCTCAAGG CGTGCACGCT AGCCCGGCGG AATGCTGACG TCTTCTTGAA GTACCTGCAC 
AGAAACAGTG TCAATATGCC CGGCATGCTG TCCCACGTCA AAGCTCCAGA GACTCAGGTT 
AAGAATATCT TGAACGAGCT GCTCCAGAGA GAGAACCGAG TCCTTCACTT CTGGACAATG 
AGGAAGAGGA GGCTGGACCA GTGTCAGCAA TATGTCGTAT TTGAACGCAG CGCCAAACAG 
GCTCTGGAGT GGATCCATGA CACTGGGGAG TTTTACTTGT CCACACACAC ATCCACTGGC 
TCCAGCATTC ATCACACACA AGAGCTGCTG AAGGAGCATG AGGACTTCCA GATCACAGCA 
AAGCAAACCA AAGAGCGAGT AAAGCTGTTG ATCCAGCTGG CAGATGGGTT TTGTGACAAG 
GGGCACGCCC ATGCACTGGA AATAAAGAAA TGGGTGTCTT CTGTGGACAA GCGCTACCGG 
GACTTCTCTC TCCGCATGGA CAAATACCGT ACCTGCCTGG AAACTGCGCT CGGCATATCA 
TCCGACTCCA ACAAGGCTAG CAAAGAGCTC CAGCTGGATA TAATCCCAGC CAGCGCTCCG 
GGCTCAGAGG TCAAACTCCG GGATGCTGCC CATGACCTCA ATGAGGAGAA GAGGAAGTCG 
GCACGGAGGA AGGAATTCAT AATGGCGGAG CTGATTCAAA CAGAAAAAGC CTACGTCAGG 
GACCTAAGGG AGTGTTTGGA TACGTACCTG TGGGAGATGA CCAGTGGTGT GGAGGAGATT 
CCCGCCGGAA TTGTCAACAA AGAGCACATC ATCTTTGGGA ACATGCAGGA CCTCTACGAG 
TTCCACCACA ACATCTTCCT GAAGGAGTTG GAGAAGTATG AGCAACTTCC AGAAGATGTC 
GGCCATTGTT TTGTCACTTG GGCTGATAAG TTCCAGATGT ACGTCAGTTA CTGTAAGAAC 
AAACCCGACT CCACGCAGCT CATCCTCGAA CATGCCGGAG CATACTTCGA TGAAATCCAG 
CAAAGACATC GCCTTGCTAA CTCCATCTCC TCTTACCTGA TCAAACCAGT GCAGAGAATC 
ACAAAGTACC AGCTGCTTCT CAAGGAGCTG CTGACATGCT GCGAGGAGGG AAAAGGTGAG 
ATCAAGGACG GACTGGAAGT GATGCTCAGC GTCCCTAAGA AAGCCAACGA CGCCATGCAC 
CTGTCTATGC TGGAGGGGTT TGATGGAAAC ATTGACTCCC AGGGAGAGCT GATACTTCAG 
GAATCCTTCC AGGTCTGGGA TCCTAAGACT CTGATCCGAA AGGGTCGGGA CCGGCACCTC 
TTCCTGTTTG AGATGTCTCT GGTGTTAAGC AAAGAAGTCA AAGATTCCAA CGGGCGCAGC 
AAATACCTGT ACAAGAGCAA GCTGTTTACA TCAGAACTTG GAGTTACGGA ACATGTGGAG 
GGAGATCCCT GTAAGTTCGC CCTGTGGGTG GGCAGGACCC CAACGTCTGA CAACAAGATC 
GTCCTCAAGG CATCCAGTAT CGAGAACAAA CAGGACTGGA TCAAACACAT CCGGGAGGTG 
ATCCAGGAGC GGATTGTCCA CCTGCGCGGC GCACTGAAGG AGCCCATCCA CATCCCCAAA 
ACCACCACAG CCAAACACAA AGGCAGACGG GATGGAGAGG AACTGGACAG CCAGGGGGAT 
GCCAGCAGCC AACCAGATAC CATCTCCATT GCCTCGCGAA CATCCCAGAA CACAATGGAC 
AGCGATAAGC TCTCCGGTGG CTGTGAGTTG ACAGTGGTGA TCCACGACTT CGTGGCCAGT 
AATGGCAGCA GCGGCGAGCT GTCGGTGCGC CGAGGTCAGA CTGTGGAAGT CCTGGAACGG 
CTCCACGACA AGCCCGACTG GTGCCTGGTG CGGACCACGG ACCGTTCCCC GGCTCAGGAG 
GGCATCGTTC CCTGCTCCAT GCTCTGCATC GCTCACTCGA GGTCTTCCAT GGAGATGGAA 
GGGCTCTTTA ACCACAAAGA CACATTATCT GTATCCAGTA ACGACGCCCT CCAACCAGGC 
TCCAGCCAAA CCCTGGGTCC TCACAGCTCC CCCGGCCCCA AACGCCCAGG GAACACACTG 
AGGAAGTGGC TGACCAGCCC CGTGCGGAGG CTCAGCTCTG GCAAGGCCGA CGGCCACGTG 
AAGAAACTGG CCCACAAGCA TAAGAAGAAC CGCGAGGGCG CGAGGAAGAA CATGGATGCC 
ATGCACGGTT CTCAGAAGGA CTCTGATGAC AGCGCCGCTA CGCCCCAGGA TGAAACGCTA 
GAGGAACGTA TGCGTAACGA AGGGCTGAGC AGCGGCACCC TGTCCAAGTC TTCCTCTTCT 
GGCATGCAGA GCTGTGGCGA GGAGGAAGGA GAGGAGGGAG CCGACGCCGT CCCTCTACCG 
CCCCCTATGG CCATCCAGCA GCACAGCTTG CTGCAGCCTG ACTCCCAGGA CGACAAGTCC 
GGATCTCGTC TGTCCGCACG TCCAAGCAGC TCAGAGACGC CCAGCGCGGC CGAACTAGTC 
AGCGCCATCG AGGAGCTGGT GAAGAGTAAG ATGTCCCTGG AGGATCGTCC CAGCTCTCTG 
TCAGTAGAAC AAGGTGACAG CAGCTCTCCC TCCCTAAATC CCTCTGACAA CTCCCTCCTC 
TCCTCCTCCT CACCCATCGA TGAGATGGAC GAGCGCAAGG CCGACTTCCT CAAGAGAAGA 
CATTACGTGC TACTGGAGTT GGTGGAGACC GAGAGGGACT ATGTCAGAGA TCTGGGATCA 
GTGGTAGAGG GCTACATGTG TAGGATGAAA GAAGAGGGTG TTCCTGATGA CATGAGGGGA 
AAAGACAAGA TCGTCTTTGG AAACATCCAT CAGATCTACG ACTGGCACAA AGATTTTTTC 
CTGGGGGAGC TTGAGAAGTG TTTAGAGGAT CCCGACAGGC TGGCACCTTT ATTCATTAAA 
CAGGAGCGGA GGCTGCACAT GTACATCGTG TACTGCCAGA ACAAACCCAA GTCTGAGCAC 
ATCGTTTCAG AGTACATCGA CACTTATTTT GAGGACCTAA AGCAGCGTTT GGGCCACAGG 
CTGCAGATCA CTGATCTATT AATCAAGCCA GTCCAGCGTA TAATGAAGTA CCAATTACTG 
CTAAAAGATC TTTTGAAAAT GTCCAAGAAG GCTGGCTTGG ATATGGCAGA GCTTGAGAAA 
GCTGTGGAGG TGATGTGCGT GGTTCCCAAA CGCTGCAACG ACATGATGAA TGTTGGACGC 
CTTCAGGGTT TTGAAGGTAA AATTGTGGCG CAGGGCAGAT TACTGCTCCA AGATACCTTC 
ATGGTGTCGG ACCAGGATGG GGGACTTCTG TCCAGGATGA AGGAGAGAAG GGTTTTCTTA 
TTTGAACAGA TAGTAATCTT TAGCGAACCT CTTGACAAAA AGAAGGGCTT CTCCACTCCA 
GGATACCTCT TTAAGAACAG CATCAAGGTG AGCTGGTTGG GTTTAGAAGA ACATTCAGAG 
GACCCTTGCA AGTTCACGCT TACCTCCAGA TCATCTAGTG GCAACTTGGA GAGATACACC 
CTCCATTCAA TGAGTCCTGG GGTCAGTCAA GTCTGGGTTC ACCAGATTAG CCAGATACTG 
GAGAACCAGC GCAACTTCCT CAATGCTTTG ACATCCCCCA TTGAATACCA GAGAAACCAT 
GTGGGTAGTG GAGTGCCAGG TGGTCTTCCC AGCAGCAGCA GCAGCAGTAC TGGAGGCCTA 
CCAGGAGGCA GCAGTTCCAT TGGCTCCTCT AGCATGGGAG TAGGAGGCGG CGGTGGCTCT 
GGGGGACCTG GGAACAGCTC ATCCTCCTCA CTCTGCGGTC CCCGTTCCCG ACCGTCCCGC 
ATTCCACAGC CATCGTCACG ACTCCCACAA CCCGTCCACC ACCACCATCC CCCGGGCCCT 
GATGGGCCTG ACAGATCAGC AGGTATGTGG CCGCCCTGCC ACCCTCAGTC CCACCCATCT 
AACTCTTATT CAGACAGGGC CACAAATGGA GAACCATTAG GCTCAGAGTT CCCCAAGATG 
AAAAGATTGG AGCTTCACGG AAATAACAGC AACAACAGCA ACAAGTCCTC AGACAGTTAT 
GAAGACACTC AGAAGCACCC GGCAGCTGGT ATACCGCAGA TGGCTGTTGC GCCTCTGAAT 
TTGACCCTAA AAAGCCCCCG AGTCGGGACA GTTTCTCCTC TGATTTCACC TCAGTCTCCT 
GCAGGAGGAA AAGAAGCATT TATCCCTTCA AGTCAAGCTC ATAAAGGCAA CTTGTTCTGG 
GCTGTGGTCC CTCCTATGCC TTCCTCTGTG TCGAGTCGGC CCAGTTCGCT CTCCTACCCC 
AGCGATAGTG TTGGTGGAGG GGATTCTCTG GGAAGAGGCG GCCACTGTAC GCCCTCTCAC 
CATCGCCAGT CCACTCACAG TAAGGACATA GACCGCATGA GCACCTGCTC CTCCACCAGC 
GAGCAGTCCA TACAGTCCAT CCAGAGCAAT GGGAGTGAAA ACAGTAGCAG CAGCATTGTG 
TCCACCATGT TGGTGACCCA GGACTATGTG GCAGTGAAGG AGGATGAGAT CAGTGTGGTC 
CAGGGTGAGG TGGTCCAAAT ACTGGCCAGC AACCAACAAA ACATGTTCCT GGTGTACCGG 
GCAGCCAACG AGCACTCTCC TGCTGCTGAG GGCTGGATCC CTGGCTATGT ATTAGGACAC 
ACCTCATCCT CTGTCACACC TGAGCTCCCT GAAGGAACCA TCAAAAAATC ACTATCATGG 
CACACAGCAC TCCGAATTCG CCGTAAATCT GAAAAAAGGG ACAAGGAGGG CAGGAAGATC 
GAGAACGGGT ACCGCAAATC TCAGGACAGC CTGGCCAACA AAGTCTCTGT TAAGCTTCTG 
AATCCCAATT TCATTCCTGA CGCACCTCCA GAATTCCTCA TCCCTGTCAC CGATGTAGCA 
TGTGAGAGCG GCGACACTGT GACACTCAGG TGCAAAATCT GTAGCCGGCC TCGCGCCACC 
GTTACCTGGC GAGGACCTGA CAACGGCGTT CGGAGCAACA ACGGACGCTA CAGCACCACC 
TACAGTGAGA CAGAAGAAGC GACTCTCCGT ATTGTGATGG TGTCCGTAGA GGACAGTGGC 
GTGTACACGT GTGTCGCCAC AAATGTTGCA GGCTCCGTCA CGTCCTCCGC CAACCTCACA 
GTCTCAGCTT CGCATGATGG CAACAGTGAG ATCTGGAAGA GCAGCTTTGA GTTCAACTAC 
ACAGAGATCA CAGAACTAGG AAGGGGCCGT TTCTCAGTGA CCAAACGGTG CGACCAGAGA 
GGGAGCAAGC GCACGGTTGC GGCCAAATAC GTTAGCAAGA AGCTGCTGCG GCGAGAGCAG 
GTGCTGCAGG AGATCAGACT CCTGCAGACT CTGGACCATC CCAACCTGGT TAAGCTTCTG 
GACACCTATG AAATGGCCAA CAGCTATGTT CTCGTGCTAG AAATGGCGGA CCAGGGACGT 
TTCTTGGATT ACGTAGTGAG CTGGGGCAAC CTCACAGAGG AGAAGGTGGC ACTGTACCTC 
AGAGAAATTC TTGAAGCTCT TCACTACCTG CACGGCTGGA GGATAGCGCA CCTCGACCTC 
AAACCTGAGA ACATTGTGGT GGAACATGCA TCCTCGCAGC CGGTGATCAA GCTGACAGAC 
TTTGGTGATG CCGTGCAACT GAATCCAGCC TCCTCGTACA TCCATCCTCT GCTGGGGAGC 
CCAGAGTTCT CTGCTCCTGA GCTGGTCCTC GGGCAGCCCT CCTCGCTGAC GTCGGACATC 
TGGAGTTTAG GGGTGGTGAC ATACATTGTA CTCAGTGGCG CTTCCCCCTT CCTGGATGAG 
AGTTTGGAGG AGACGTGTCT GAACATCTGT CGCCTGGACT TCAGCTTCCC GGAGGACTAC 
TTCCAGGGAG TGAGCCTGGC TGCCAAGGAC TTTGTCTGCC TGCTGCTCCA GAGCGAGCCG 
GAGCGGCGGC CCACTGCGTT AGCCTGCCTG CAGGAGCAGT GGCTCCAGCC CCGAGGTGTG 
ATCAATACTG GATTTGTAGG CTCGAAACAT GCACACGCGT CGCCCAACCA TCACACTTTA 
CATCTGGACA CATCCAGACT CATTTCTTTC ATTGAGAGGC GGAAACACCA GAACGACATT 
CGGCCCATAG ACACGGTCAG GGCCTTCCTC CACAGCCGCC TGCTTAACAA CATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029695079.1
CDS	49..9405
Translation	MAVLDNHSWQYAQAGKMLIRLSGFHRNEDMKAIDVLPILKEKVAFLSGGRDRRGGPVLTFPARSNHDRIRPEDLRRLIAYLATIPSEEVARHGFTVIVDMRGSKWDSIKPLLKILQESFPSCIHVALIIKPDNFWQKQRTNFGSSKFEFETVMVSLEGLSKIVDPSQLTADFEGSLEYNHDDWIEVRLSFETFASDAARALARLEELQDTLSQRDLPRDLEGARRLMEEHAALKKRATKASVEELDTQGRRLLQRLQAQATGGGSVNESGYINRLGGASGDPNDHHHGCHTHADAHNLITKVSSLLEKLHGTRQNLQQLWHMRKLKLDQCFQLRLFEQDAEKMFDWIMHNKGLFLTSYTEIGGNHQHAVELQTQHNHFAMNCMNVYVNINRIMSVGNRLLESGHYASQQIQQISGQLEQEWKAFAAALDERSTLLEMSASFHQKADQYMGKVEPWCKACGEGELPSELQDLEDTIHHHQGLYEHITTAYSEVSQDGKSLLDKLQRPLTPGSADSLMASANYSKAVHHVLDIIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKVLLDMSVAFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQATVNVIKEGEDLIQQLRDSAISSNKTPHNSSMAHIESVLQQLDEAQGQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNEELSQQMSDFDTEDLTLAEQRLQHHADKALTMNNLTYDVIHQGQELLQYVTEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRRHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQKEHEQFQHAIEKTHQSALQVQQKAETLLQANHYDMDMIRDCAEKVADHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSESDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMLSHVKAPETQVKNILNELLQRENRVLHFWTMRKRRLDQCQQYVVFERSAKQALEWIHDTGEFYLSTHTSTGSSIHHTQELLKEHEDFQITAKQTKERVKLLIQLADGFCDKGHAHALEIKKWVSSVDKRYRDFSLRMDKYRTCLETALGISSDSNKASKELQLDIIPASAPGSEVKLRDAAHDLNEEKRKSARRKEFIMAELIQTEKAYVRDLRECLDTYLWEMTSGVEEIPAGIVNKEHIIFGNMQDLYEFHHNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVSYCKNKPDSTQLILEHAGAYFDEIQQRHRLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKKANDAMHLSMLEGFDGNIDSQGELILQESFQVWDPKTLIRKGRDRHLFLFEMSLVLSKEVKDSNGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERIVHLRGALKEPIHIPKTTTAKHKGRRDGEELDSQGDASSQPDTISIASRTSQNTMDSDKLSGGCELTVVIHDFVASNGSSGELSVRRGQTVEVLERLHDKPDWCLVRTTDRSPAQEGIVPCSMLCIAHSRSSMEMEGLFNHKDTLSVSSNDALQPGSSQTLGPHSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKNREGARKNMDAMHGSQKDSDDSAATPQDETLEERMRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKSGSRLSARPSSSETPSAAELVSAIEELVKSKMSLEDRPSSLSVEQGDSSSPSLNPSDNSLLSSSSPIDEMDERKADFLKRRHYVLLELVETERDYVRDLGSVVEGYMCRMKEEGVPDDMRGKDKIVFGNIHQIYDWHKDFFLGELEKCLEDPDRLAPLFIKQERRLHMYIVYCQNKPKSEHIVSEYIDTYFEDLKQRLGHRLQITDLLIKPVQRIMKYQLLLKDLLKMSKKAGLDMAELEKAVEVMCVVPKRCNDMMNVGRLQGFEGKIVAQGRLLLQDTFMVSDQDGGLLSRMKERRVFLFEQIVIFSEPLDKKKGFSTPGYLFKNSIKVSWLGLEEHSEDPCKFTLTSRSSSGNLERYTLHSMSPGVSQVWVHQISQILENQRNFLNALTSPIEYQRNHVGSGVPGGLPSSSSSSTGGLPGGSSSIGSSSMGVGGGGGSGGPGNSSSSSLCGPRSRPSRIPQPSSRLPQPVHHHHPPGPDGPDRSAGMWPPCHPQSHPSNSYSDRATNGEPLGSEFPKMKRLELHGNNSNNSNKSSDSYEDTQKHPAAGIPQMAVAPLNLTLKSPRVGTVSPLISPQSPAGGKEAFIPSSQAHKGNLFWAVVPPMPSSVSSRPSSLSYPSDSVGGGDSLGRGGHCTPSHHRQSTHSKDIDRMSTCSSTSEQSIQSIQSNGSENSSSSIVSTMLVTQDYVAVKEDEISVVQGEVVQILASNQQNMFLVYRAANEHSPAAEGWIPGYVLGHTSSSVTPELPEGTIKKSLSWHTALRIRRKSEKRDKEGRKIENGYRKSQDSLANKVSVKLLNPNFIPDAPPEFLIPVTDVACESGDTVTLRCKICSRPRATVTWRGPDNGVRSNNGRYSTTYSETEEATLRIVMVSVEDSGVYTCVATNVAGSVTSSANLTVSASHDGNSEIWKSSFEFNYTEITELGRGRFSVTKRCDQRGSKRTVAAKYVSKKLLRREQVLQEIRLLQTLDHPNLVKLLDTYEMANSYVLVLEMADQGRFLDYVVSWGNLTEEKVALYLREILEALHYLHGWRIAHLDLKPENIVVEHASSQPVIKLTDFGDAVQLNPASSYIHPLLGSPEFSAPELVLGQPSSLTSDIWSLGVVTYIVLSGASPFLDESLEETCLNICRLDFSFPEDYFQGVSLAAKDFVCLLLQSEPERRPTALACLQEQWLQPRGVINTGFVGSKHAHASPNHHTLHLDTSRLISFIERRKHQNDIRPIDTVRAFLHSRLLNNI

Target ORF information:

RefSeq Version	XM_029839219.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes triple functional domain protein-like (LOC101071578), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029839219.1

ORF Insert Sequence:

ATGGCAGTGT TGGATAACCA CAGCTGGCAG TATGCCCAAG CTGGGAAAAT GCTCATCAGA 
TTATCAGGGT TTCACCGCAA TGAGGATATG AAGGCCATTG ACGTACTTCC TATCCTCAAG 
GAGAAGGTCG CCTTTCTCTC AGGTGGCAGA GACAGACGCG GAGGTCCCGT TCTCACATTT 
CCTGCTCGGA GCAACCATGA CAGAATACGA CCTGAAGACC TCCGCAGGCT CATAGCTTAC 
CTGGCTACTA TTCCCAGCGA GGAAGTGGCG AGGCATGGCT TCACGGTCAT TGTGGACATG 
CGTGGCTCTA AATGGGACAG CATCAAGCCT TTGCTGAAGA TCCTTCAGGA GTCGTTCCCA 
TCTTGCATCC ACGTTGCCCT CATCATCAAG CCCGACAACT TCTGGCAGAA GCAGAGGACA 
AACTTCGGCA GCTCCAAATT TGAATTCGAG ACGGTGATGG TCTCTTTAGA GGGTTTATCC 
AAAATCGTGG ACCCATCACA GCTGACAGCT GACTTTGAGG GCAGCCTGGA ATACAACCAT 
GATGACTGGA TCGAGGTGCG GCTGTCCTTT GAGACGTTTG CCAGCGATGC GGCACGTGCG 
CTGGCACGCC TGGAGGAGCT CCAGGACACG TTGTCCCAGC GTGATCTCCC GCGGGACCTG 
GAAGGAGCTC GGCGGCTCAT GGAGGAACAC GCGGCGCTCA AGAAAAGGGC CACGAAAGCA 
TCCGTGGAGG AGCTGGACAC GCAGGGGCGA CGGCTGCTGC AGAGGCTGCA GGCACAGGCT 
ACGGGAGGAG GCAGCGTTAA TGAAAGCGGG TACATTAACC GCCTGGGCGG AGCTAGCGGA 
GACCCCAACG ACCACCATCA CGGGTGCCAC ACGCACGCAG ACGCACACAA CCTCATCACC 
AAAGTGAGCA GCTTGTTGGA AAAGCTTCAC GGGACTCGGC AAAATCTGCA GCAGCTGTGG 
CACATGAGGA AGCTGAAGCT GGACCAGTGT TTCCAGCTGC GTCTTTTTGA ACAGGACGCA 
GAGAAGATGT TCGACTGGAT CATGCACAAC AAGGGGCTTT TTCTCACGAG TTACACGGAG 
ATCGGGGGGA ACCACCAGCA CGCTGTCGAG CTGCAGACCC AACACAACCA CTTTGCCATG 
AACTGCATGA ATGTGTACGT GAACATCAAC CGGATCATGT CAGTCGGGAA CCGGCTCCTG 
GAGTCGGGCC ATTACGCCTC GCAGCAGATC CAGCAGATCT CCGGGCAGCT GGAGCAGGAG 
TGGAAGGCCT TCGCTGCGGC GCTGGATGAA CGCAGCACGC TGCTGGAGAT GTCCGCCAGC 
TTCCACCAGA AAGCGGACCA GTACATGGGG AAAGTGGAAC CGTGGTGTAA AGCCTGTGGT 
GAAGGAGAGC TGCCCTCTGA ACTCCAGGAT CTGGAAGACA CAATCCACCA TCATCAGGGG 
CTTTATGAGC ACATCACCAC TGCTTACTCT GAGGTAAGCC AGGATGGGAA GTCACTCTTG 
GACAAGCTGC AGCGCCCTTT GACCCCAGGG AGCGCTGATT CCTTGATGGC CTCTGCCAAC 
TACTCCAAGG CCGTGCATCA CGTCTTAGAC ATCATCCACG AGGTGCTGCA CCACCAGAGG 
CAGCTGGAAA ACATCTGGCA GCATCGCAAA GTCCGCCTGC ACCAACGGCT GCAACTGTGT 
GTTTTTCAGC AGGATGTGCA GCAGGTACTG GACTGGATAG AAAACCACGG AGAAGCCTTC 
CTAAGCAAAC ACACTGGTGT TGGCAAGTCT CTGCACAGAG CCAGAGCTCT GCAGAAGAGG 
CATGAGGATT TTGAAGAGGT TGCACAGAAC ACCTACACTA ATGCTGACAA ACTGCTGGAA 
GCCGCAGAGC AGCTGGCTCA GACCGGCGAA TGTGATCCAG AGGAAATTTA CCAGGCTGCC 
CACCAGCTGG AAGATCGCAT CCAGGACTTC GTACGACGTG TCGAGCAGAG AAAAGTCCTG 
TTGGACATGT CTGTCGCCTT CCACACGCAT GTCAAAGAGC TGTGGACGTG GCTGGAGGAG 
TTGCAGAAGG AATTGCTAGA TGACGTATAC GCAGAATCCG TGGAGGCTGT TCAGGACCTG 
ATCAAACGCT TCGGCCAGCA GCAGCAGACC ACCCTGCAGG CTACTGTCAA TGTCATCAAA 
GAGGGAGAGG ACCTTATCCA ACAGCTCAGA GACTCGGCTA TCTCAAGCAA CAAAACTCCC 
CATAACAGCT CCATGGCCCA CATCGAGAGT GTGCTGCAGC AGCTGGACGA GGCTCAGGGC 
CAGATGGAGG AACTGTTTCA GGAAAGGAAG ATCAAACTGG AGCTCTTTCT GCAGCTGCGC 
ATCTTCGAGA GGGACGCCAT CGATATCATC TCTGACCTGG AGTCATGGAA CGAGGAGCTC 
TCCCAGCAGA TGAGTGACTT CGACACCGAG GATCTGACGC TGGCGGAGCA GAGGCTGCAG 
CACCACGCAG ACAAGGCGCT CACCATGAAC AACCTGACCT ACGACGTCAT CCACCAGGGC 
CAAGAGCTGC TGCAGTACGT GACCGAGGTC CAGGCGTCTG GTGTGGAGCT GCTGTGCGAC 
AGAGATGTGG ACATGGCCAC ACGGGTCCAG GACCTGCTGG AGTTCCTCCA CGAGAAGCAG 
CAGGAGCTGG ACCTGGCGGC GGAGCAGCAC AGGAGACACT TGGAACAGTG TGTCCAACTG 
AGACACCTCC AGGCAGAAGT CAAACAGGTG CTGGGATGGA TCCGGAATGG CGAGTCCATG 
CTGAACGCTG GTCTGATCAC AGCCAGCTCG TTACAGGAAG CTGAACAGCT GCAGAAGGAA 
CATGAACAAT TTCAGCATGC AATAGAGAAA ACTCATCAGA GCGCGCTGCA GGTGCAGCAG 
AAGGCCGAGA CCTTACTCCA GGCAAACCAC TATGACATGG ACATGATCAG GGACTGTGCA 
GAAAAGGTGG CAGACCACTG GCAGCAGCTC ATGCTGAAAA TGGAAGACCG ACTCAAGCTG 
GTCAACGCTT CTGTGGCCTT CTACAAAACC TCCGAACAGG TTTGCAGTGT GTTGGAAAGC 
CTGGAGCAGG AGTACAAGAG AGAAGAGGAC TGGTGTGGTG GCGCTGATAA ACTAGGCCCC 
AACAGCGAGT CCGACCACGT CACTCCCATG ATCAGCAAAC ATCTGGAGCA GAAAGAGGCC 
TTTCTCAAGG CGTGCACGCT AGCCCGGCGG AATGCTGACG TCTTCTTGAA GTACCTGCAC 
AGAAACAGTG TCAATATGCC CGGCATGCTG TCCCACGTCA AAGCTCCAGA GACTCAGGTT 
AAGAATATCT TGAACGAGCT GCTCCAGAGA GAGAACCGAG TCCTTCACTT CTGGACAATG 
AGGAAGAGGA GGCTGGACCA GTGTCAGCAA TATGTCGTAT TTGAACGCAG CGCCAAACAG 
GCTCTGGAGT GGATCCATGA CACTGGGGAG TTTTACTTGT CCACACACAC ATCCACTGGC 
TCCAGCATTC ATCACACACA AGAGCTGCTG AAGGAGCATG AGGACTTCCA GATCACAGCA 
AAGCAAACCA AAGAGCGAGT AAAGCTGTTG ATCCAGCTGG CAGATGGGTT TTGTGACAAG 
GGGCACGCCC ATGCACTGGA AATAAAGAAA TGGGTGTCTT CTGTGGACAA GCGCTACCGG 
GACTTCTCTC TCCGCATGGA CAAATACCGT ACCTGCCTGG AAACTGCGCT CGGCATATCA 
TCCGACTCCA ACAAGGCTAG CAAAGAGCTC CAGCTGGATA TAATCCCAGC CAGCGCTCCG 
GGCTCAGAGG TCAAACTCCG GGATGCTGCC CATGACCTCA ATGAGGAGAA GAGGAAGTCG 
GCACGGAGGA AGGAATTCAT AATGGCGGAG CTGATTCAAA CAGAAAAAGC CTACGTCAGG 
GACCTAAGGG AGTGTTTGGA TACGTACCTG TGGGAGATGA CCAGTGGTGT GGAGGAGATT 
CCCGCCGGAA TTGTCAACAA AGAGCACATC ATCTTTGGGA ACATGCAGGA CCTCTACGAG 
TTCCACCACA ACATCTTCCT GAAGGAGTTG GAGAAGTATG AGCAACTTCC AGAAGATGTC 
GGCCATTGTT TTGTCACTTG GGCTGATAAG TTCCAGATGT ACGTCAGTTA CTGTAAGAAC 
AAACCCGACT CCACGCAGCT CATCCTCGAA CATGCCGGAG CATACTTCGA TGAAATCCAG 
CAAAGACATC GCCTTGCTAA CTCCATCTCC TCTTACCTGA TCAAACCAGT GCAGAGAATC 
ACAAAGTACC AGCTGCTTCT CAAGGAGCTG CTGACATGCT GCGAGGAGGG AAAAGGTGAG 
ATCAAGGACG GACTGGAAGT GATGCTCAGC GTCCCTAAGA AAGCCAACGA CGCCATGCAC 
CTGTCTATGC TGGAGGGGTT TGATGGAAAC ATTGACTCCC AGGGAGAGCT GATACTTCAG 
GAATCCTTCC AGGTCTGGGA TCCTAAGACT CTGATCCGAA AGGGTCGGGA CCGGCACCTC 
TTCCTGTTTG AGATGTCTCT GGTGTTAAGC AAAGAAGTCA AAGATTCCAA CGGGCGCAGC 
AAATACCTGT ACAAGAGCAA GCTGTTTACA TCAGAACTTG GAGTTACGGA ACATGTGGAG 
GGAGATCCCT GTAAGTTCGC CCTGTGGGTG GGCAGGACCC CAACGTCTGA CAACAAGATC 
GTCCTCAAGG CATCCAGTAT CGAGAACAAA CAGGACTGGA TCAAACACAT CCGGGAGGTG 
ATCCAGGAGC GGATTGTCCA CCTGCGCGGC GCACTGAAGG AGCCCATCCA CATCCCCAAA 
ACCACCACAG CCAAACACAA AGGCAGACGG GATGGAGAGG AACTGGACAG CCAGGGGGAT 
GCCAGCAGCC AACCAGATAC CATCTCCATT GCCTCGCGAA CATCCCAGAA CACAATGGAC 
AGCGATAAGC TCTCCGGTGG CTGTGAGTTG ACAGTGGTGA TCCACGACTT CGTGGCCAGT 
AATGGCAGCA GCGGCGAGCT GTCGGTGCGC CGAGGTCAGA CTGTGGAAGT CCTGGAACGG 
CTCCACGACA AGCCCGACTG GTGCCTGGTG CGGACCACGG ACCGTTCCCC GGCTCAGGAG 
GGCATCGTTC CCTGCTCCAT GCTCTGCATC GCTCACTCGA GGTCTTCCAT GGAGATGGAA 
GGGCTCTTTA ACCACAAAGA CACATTATCT GTATCCAGTA ACGACGCCCT CCAACCAGGC 
TCCAGCCAAA CCCTGGGTCC TCACAGCTCC CCCGGCCCCA AACGCCCAGG GAACACACTG 
AGGAAGTGGC TGACCAGCCC CGTGCGGAGG CTCAGCTCTG GCAAGGCCGA CGGCCACGTG 
AAGAAACTGG CCCACAAGCA TAAGAAGAAC CGCGAGGGCG CGAGGAAGAA CATGGATGCC 
ATGCACGGTT CTCAGAAGGA CTCTGATGAC AGCGCCGCTA CGCCCCAGGA TGAAACGCTA 
GAGGAACGTA TGCGTAACGA AGGGCTGAGC AGCGGCACCC TGTCCAAGTC TTCCTCTTCT 
GGCATGCAGA GCTGTGGCGA GGAGGAAGGA GAGGAGGGAG CCGACGCCGT CCCTCTACCG 
CCCCCTATGG CCATCCAGCA GCACAGCTTG CTGCAGCCTG ACTCCCAGGA CGACAAGTCC 
GGATCTCGTC TGTCCGCACG TCCAAGCAGC TCAGAGACGC CCAGCGCGGC CGAACTAGTC 
AGCGCCATCG AGGAGCTGGT GAAGAGTAAG ATGTCCCTGG AGGATCGTCC CAGCTCTCTG 
TCAGTAGAAC AAGGTGACAG CAGCTCTCCC TCCCTAAATC CCTCTGACAA CTCCCTCCTC 
TCCTCCTCCT CACCCATCGA TGAGATGGAC GAGCGCAAGG CCGACTTCCT CAAGAGAAGA 
CATTACGTGC TACTGGAGTT GGTGGAGACC GAGAGGGACT ATGTCAGAGA TCTGGGATCA 
GTGGTAGAGG GCTACATGTG TAGGATGAAA GAAGAGGGTG TTCCTGATGA CATGAGGGGA 
AAAGACAAGA TCGTCTTTGG AAACATCCAT CAGATCTACG ACTGGCACAA AGATTTTTTC 
CTGGGGGAGC TTGAGAAGTG TTTAGAGGAT CCCGACAGGC TGGCACCTTT ATTCATTAAA 
CAGGAGCGGA GGCTGCACAT GTACATCGTG TACTGCCAGA ACAAACCCAA GTCTGAGCAC 
ATCGTTTCAG AGTACATCGA CACTTATTTT GAGGACCTAA AGCAGCGTTT GGGCCACAGG 
CTGCAGATCA CTGATCTATT AATCAAGCCA GTCCAGCGTA TAATGAAGTA CCAATTACTG 
CTAAAAGATC TTTTGAAAAT GTCCAAGAAG GCTGGCTTGG ATATGGCAGA GCTTGAGAAA 
GCTGTGGAGG TGATGTGCGT GGTTCCCAAA CGCTGCAACG ACATGATGAA TGTTGGACGC 
CTTCAGGGTT TTGAAGGTAA AATTGTGGCG CAGGGCAGAT TACTGCTCCA AGATACCTTC 
ATGGTGTCGG ACCAGGATGG GGGACTTCTG TCCAGGATGA AGGAGAGAAG GGTTTTCTTA 
TTTGAACAGA TAGTAATCTT TAGCGAACCT CTTGACAAAA AGAAGGGCTT CTCCACTCCA 
GGATACCTCT TTAAGAACAG CATCAAGGTG AGCTGGTTGG GTTTAGAAGA ACATTCAGAG 
GACCCTTGCA AGTTCACGCT TACCTCCAGA TCATCTAGTG GCAACTTGGA GAGATACACC 
CTCCATTCAA TGAGTCCTGG GGTCAGTCAA GTCTGGGTTC ACCAGATTAG CCAGATACTG 
GAGAACCAGC GCAACTTCCT CAATGCTTTG ACATCCCCCA TTGAATACCA GAGAAACCAT 
GTGGGTAGTG GAGTGCCAGG TGGTCTTCCC AGCAGCAGCA GCAGCAGTAC TGGAGGCCTA 
CCAGGAGGCA GCAGTTCCAT TGGCTCCTCT AGCATGGGAG TAGGAGGCGG CGGTGGCTCT 
GGGGGACCTG GGAACAGCTC ATCCTCCTCA CTCTGCGGTC CCCGTTCCCG ACCGTCCCGC 
ATTCCACAGC CATCGTCACG ACTCCCACAA CCCGTCCACC ACCACCATCC CCCGGGCCCT 
GATGGGCCTG ACAGATCAGC AGGTATGTGG CCGCCCTGCC ACCCTCAGTC CCACCCATCT 
AACTCTTATT CAGACAGGGC CACAAATGGA GAACCATTAG GCTCAGAGTT CCCCAAGATG 
AAAAGATTGG AGCTTCACGG AAATAACAGC AACAACAGCA ACAAGTCCTC AGACAGTTAT 
GAAGACACTC AGAAGCACCC GGCAGCTGGT ATACCGCAGA TGGCTGTTGC GCCTCTGAAT 
TTGACCCTAA AAAGCCCCCG AGTCGGGACA GTTTCTCCTC TGATTTCACC TCAGTCTCCT 
GCAGGAGGAA AAGAAGCATT TATCCCTTCA AGTCAAGCTC ATAAAGGCAA CTTGTTCTGG 
GCTGTGGTCC CTCCTATGCC TTCCTCTGTG TCGAGTCGGC CCAGTTCGCT CTCCTACCCC 
AGCGATAGTG TTGGTGGAGG GGATTCTCTG GGAAGAGGCG GCCACTGTAC GCCCTCTCAC 
CATCGCCAGT CCACTCACAG TAAGGACATA GACCGCATGA GCACCTGCTC CTCCACCAGC 
GAGCAGTCCA TACAGTCCAT CCAGAGCAAT GGGAGTGAAA ACAGTAGCAG CAGCATTGTG 
TCCACCATGT TGGTGACCCA GGACTATGTG GCAGTGAAGG AGGATGAGAT CAGTGTGGTC 
CAGGGTGAGG TGGTCCAAAT ACTGGCCAGC AACCAACAAA ACATGTTCCT GGTGTACCGG 
GCAGCCAACG AGCACTCTCC TGCTGCTGAG GGCTGGATCC CTGGCTATGT ATTAGGACAC 
ACCTCATCCT CTGTCACACC TGAGCTCCCT GAAGGAACCA TCAAAAAATC ACTATCATGG 
CACACAGCAC TCCGAATTCG CCGTAAATCT GAAAAAAGGG ACAAGGAGGG CAGGAAGATC 
GAGAACGGGT ACCGCAAATC TCAGGACAGC CTGGCCAACA AAGTCTCTGT TAAGCTTCTG 
AATCCCAATT TCATTCCTGA CGCACCTCCA GAATTCCTCA TCCCTGTCAC CGATGTAGCA 
TGTGAGAGCG GCGACACTGT GACACTCAGG TGCAAAATCT GTAGCCGGCC TCGCGCCACC 
GTTACCTGGC GAGGACCTGA CAACGGCGTT CGGAGCAACA ACGGACGCTA CAGCACCACC 
TACAGTGAGA CAGAAGAAGC GACTCTCCGT ATTGTGATGG TGTCCGTAGA GGACAGTGGC 
GTGTACACGT GTGTCGCCAC AAATGTTGCA GGCTCCGTCA CGTCCTCCGC CAACCTCACA 
GTCTCAGCTT CGCATGATGG CAACAGTGAG ATCTGGAAGA GCAGCTTTGA GTTCAACTAC 
ACAGAGATCA CAGAACTAGG AAGGGGCCGT TTCTCAGTGA CCAAACGGTG CGACCAGAGA 
GGGAGCAAGC GCACGGTTGC GGCCAAATAC GTTAGCAAGA AGCTGCTGCG GCGAGAGCAG 
GTGCTGCAGG AGATCAGACT CCTGCAGACT CTGGACCATC CCAACCTGGT TAAGCTTCTG 
GACACCTATG AAATGGCCAA CAGCTATGTT CTCGTGCTAG AAATGGCGGA CCAGGGACGT 
TTCTTGGATT ACGTAGTGAG CTGGGGCAAC CTCACAGAGG AGAAGGTGGC ACTGTACCTC 
AGAGAAATTC TTGAAGCTCT TCACTACCTG CACGGCTGGA GGATAGCGCA CCTCGACCTC 
AAACCTGAGA ACATTGTGGT GGAACATGCA TCCTCGCAGC CGGTGATCAA GCTGACAGAC 
TTTGGTGATG CCGTGCAACT GAATCCAGCC TCCTCGTACA TCCATCCTCT GCTGGGGAGC 
CCAGAGTTCT CTGCTCCTGA GCTGGTCCTC GGGCAGCCCT CCTCGCTGAC GTCGGACATC 
TGGAGTTTAG GGGTGGTGAC ATACATTGTA CTCAGTGGCG CTTCCCCCTT CCTGGATGAG 
AGTTTGGAGG AGACGTGTCT GAACATCTGT CGCCTGGACT TCAGCTTCCC GGAGGACTAC 
TTCCAGGGAG TGAGCCTGGC TGCCAAGGAC TTTGTCTGCC TGCTGCTCCA GAGCGAGCCG 
GAGCGGCGGC CCACTGCGTT AGCCTGCCTG CAGGAGCAGT GGCTCCAGCC CCGAGGTGTG 
ATCAATACTG GATTTGTAGG CTCGAAACAT GCACACGCGT CGCCCAACCA TCACACTTTA 
CATCTGGACA CATCCAGACT CATTTCTTTC ATTGAGAGGC GGAAACACCA GAACGACATT 
CGGCCCATAG ACACGGTCAG GGCCTTCCTC CACAGCCGCC TGCTTAACAA CATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101071578 ( XM_029839220.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029695080.1 Takifugu rubripes triple functional domain protein-like (LOC101071578), transcript variant X4, mRNA.

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CloneID	OTo69930
Clone ID Related Accession (Same CDS sequence)	XM_029839220.1
Accession Version	XM_029839220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	triple functional domain protein-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042291.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCAG GAGAGGGATT AGATGAGGCT GCGAAGGACG CGGCAGACAT AGCGGCGTTT 
TTCAAATCCG GGTTTCACCG CAATGAGGAT ATGAAGGCCA TTGACGTACT TCCTATCCTC 
AAGGAGAAGG TCGCCTTTCT CTCAGGTGGC AGAGACAGAC GCGGAGGTCC CGTTCTCACA 
TTTCCTGCTC GGAGCAACCA TGACAGAATA CGACCTGAAG ACCTCCGCAG GCTCATAGCT 
TACCTGGCTA CTATTCCCAG CGAGGAAGTG GCGAGGCATG GCTTCACGGT CATTGTGGAC 
ATGCGTGGCT CTAAATGGGA CAGCATCAAG CCTTTGCTGA AGATCCTTCA GGAGTCGTTC 
CCATCTTGCA TCCACGTTGC CCTCATCATC AAGCCCGACA ACTTCTGGCA GAAGCAGAGG 
ACAAACTTCG GCAGCTCCAA ATTTGAATTC GAGACGGTGA TGGTCTCTTT AGAGGGTTTA 
TCCAAAATCG TGGACCCATC ACAGCTGACA GCTGACTTTG AGGGCAGCCT GGAATACAAC 
CATGATGACT GGATCGAGGT GCGGCTGTCC TTTGAGACGT TTGCCAGCGA TGCGGCACGT 
GCGCTGGCAC GCCTGGAGGA GCTCCAGGAC ACGTTGTCCC AGCGTGATCT CCCGCGGGAC 
CTGGAAGGAG CTCGGCGGCT CATGGAGGAA CACGCGGCGC TCAAGAAAAG GGCCACGAAA 
GCATCCGTGG AGGAGCTGGA CACGCAGGGG CGACGGCTGC TGCAGAGGCT GCAGGCACAG 
GCTACGGGAG GAGGCAGCGT TAATGAAAGC GGGTACATTA ACCGCCTGGG CGGAGCTAGC 
GGAGACCCCA ACGACCACCA TCACGGGTGC CACACGCACG CAGACGCACA CAACCTCATC 
ACCAAAGTGA GCAGCTTGTT GGAAAAGCTT CACGGGACTC GGCAAAATCT GCAGCAGCTG 
TGGCACATGA GGAAGCTGAA GCTGGACCAG TGTTTCCAGC TGCGTCTTTT TGAACAGGAC 
GCAGAGAAGA TGTTCGACTG GATCATGCAC AACAAGGGGC TTTTTCTCAC GAGTTACACG 
GAGATCGGGG GGAACCACCA GCACGCTGTC GAGCTGCAGA CCCAACACAA CCACTTTGCC 
ATGAACTGCA TGAATGTGTA CGTGAACATC AACCGGATCA TGTCAGTCGG GAACCGGCTC 
CTGGAGTCGG GCCATTACGC CTCGCAGCAG ATCCAGCAGA TCTCCGGGCA GCTGGAGCAG 
GAGTGGAAGG CCTTCGCTGC GGCGCTGGAT GAACGCAGCA CGCTGCTGGA GATGTCCGCC 
AGCTTCCACC AGAAAGCGGA CCAGTACATG GGGAAAGTGG AACCGTGGTG TAAAGCCTGT 
GGTGAAGGAG AGCTGCCCTC TGAACTCCAG GATCTGGAAG ACACAATCCA CCATCATCAG 
GGGCTTTATG AGCACATCAC CACTGCTTAC TCTGAGGTAA GCCAGGATGG GAAGTCACTC 
TTGGACAAGC TGCAGCGCCC TTTGACCCCA GGGAGCGCTG ATTCCTTGAT GGCCTCTGCC 
AACTACTCCA AGGCCGTGCA TCACGTCTTA GACATCATCC ACGAGGTGCT GCACCACCAG 
AGGCAGCTGG AAAACATCTG GCAGCATCGC AAAGTCCGCC TGCACCAACG GCTGCAACTG 
TGTGTTTTTC AGCAGGATGT GCAGCAGGTA CTGGACTGGA TAGAAAACCA CGGAGAAGCC 
TTCCTAAGCA AACACACTGG TGTTGGCAAG TCTCTGCACA GAGCCAGAGC TCTGCAGAAG 
AGGCATGAGG ATTTTGAAGA GGTTGCACAG AACACCTACA CTAATGCTGA CAAACTGCTG 
GAAGCCGCAG AGCAGCTGGC TCAGACCGGC GAATGTGATC CAGAGGAAAT TTACCAGGCT 
GCCCACCAGC TGGAAGATCG CATCCAGGAC TTCGTACGAC GTGTCGAGCA GAGAAAAGTC 
CTGTTGGACA TGTCTGTCGC CTTCCACACG CATGTCAAAG AGCTGTGGAC GTGGCTGGAG 
GAGTTGCAGA AGGAATTGCT AGATGACGTA TACGCAGAAT CCGTGGAGGC TGTTCAGGAC 
CTGATCAAAC GCTTCGGCCA GCAGCAGCAG ACCACCCTGC AGGCTACTGT CAATGTCATC 
AAAGAGGGAG AGGACCTTAT CCAACAGCTC AGAGACTCGG CTATCTCAAG CAACAAAACT 
CCCCATAACA GCTCCATGGC CCACATCGAG AGTGTGCTGC AGCAGCTGGA CGAGGCTCAG 
GGCCAGATGG AGGAACTGTT TCAGGAAAGG AAGATCAAAC TGGAGCTCTT TCTGCAGCTG 
CGCATCTTCG AGAGGGACGC CATCGATATC ATCTCTGACC TGGAGTCATG GAACGAGGAG 
CTCTCCCAGC AGATGAGTGA CTTCGACACC GAGGATCTGA CGCTGGCGGA GCAGAGGCTG 
CAGCACCACG CAGACAAGGC GCTCACCATG AACAACCTGA CCTACGACGT CATCCACCAG 
GGCCAAGAGC TGCTGCAGTA CGTGACCGAG GTCCAGGCGT CTGGTGTGGA GCTGCTGTGC 
GACAGAGATG TGGACATGGC CACACGGGTC CAGGACCTGC TGGAGTTCCT CCACGAGAAG 
CAGCAGGAGC TGGACCTGGC GGCGGAGCAG CACAGGAGAC ACTTGGAACA GTGTGTCCAA 
CTGAGACACC TCCAGGCAGA AGTCAAACAG GTGCTGGGAT GGATCCGGAA TGGCGAGTCC 
ATGCTGAACG CTGGTCTGAT CACAGCCAGC TCGTTACAGG AAGCTGAACA GCTGCAGAAG 
GAACATGAAC AATTTCAGCA TGCAATAGAG AAAACTCATC AGAGCGCGCT GCAGGTGCAG 
CAGAAGGCCG AGACCTTACT CCAGGCAAAC CACTATGACA TGGACATGAT CAGGGACTGT 
GCAGAAAAGG TGGCAGACCA CTGGCAGCAG CTCATGCTGA AAATGGAAGA CCGACTCAAG 
CTGGTCAACG CTTCTGTGGC CTTCTACAAA ACCTCCGAAC AGGTTTGCAG TGTGTTGGAA 
AGCCTGGAGC AGGAGTACAA GAGAGAAGAG GACTGGTGTG GTGGCGCTGA TAAACTAGGC 
CCCAACAGCG AGTCCGACCA CGTCACTCCC ATGATCAGCA AACATCTGGA GCAGAAAGAG 
GCCTTTCTCA AGGCGTGCAC GCTAGCCCGG CGGAATGCTG ACGTCTTCTT GAAGTACCTG 
CACAGAAACA GTGTCAATAT GCCCGGCATG CTGTCCCACG TCAAAGCTCC AGAGACTCAG 
GTTAAGAATA TCTTGAACGA GCTGCTCCAG AGAGAGAACC GAGTCCTTCA CTTCTGGACA 
ATGAGGAAGA GGAGGCTGGA CCAGTGTCAG CAATATGTCG TATTTGAACG CAGCGCCAAA 
CAGGCTCTGG AGTGGATCCA TGACACTGGG GAGTTTTACT TGTCCACACA CACATCCACT 
GGCTCCAGCA TTCATCACAC ACAAGAGCTG CTGAAGGAGC ATGAGGACTT CCAGATCACA 
GCAAAGCAAA CCAAAGAGCG AGTAAAGCTG TTGATCCAGC TGGCAGATGG GTTTTGTGAC 
AAGGGGCACG CCCATGCACT GGAAATAAAG AAATGGGTGT CTTCTGTGGA CAAGCGCTAC 
CGGGACTTCT CTCTCCGCAT GGACAAATAC CGTACCTGCC TGGAAACTGC GCTCGGCATA 
TCATCCGACT CCAACAAGGC TAGCAAAGAG CTCCAGCTGG ATATAATCCC AGCCAGCGCT 
CCGGGCTCAG AGGTCAAACT CCGGGATGCT GCCCATGACC TCAATGAGGA GAAGAGGAAG 
TCGGCACGGA GGAAGGAATT CATAATGGCG GAGCTGATTC AAACAGAAAA AGCCTACGTC 
AGGGACCTAA GGGAGTGTTT GGATACGTAC CTGTGGGAGA TGACCAGTGG TGTGGAGGAG 
ATTCCCGCCG GAATTGTCAA CAAAGAGCAC ATCATCTTTG GGAACATGCA GGACCTCTAC 
GAGTTCCACC ACAACATCTT CCTGAAGGAG TTGGAGAAGT ATGAGCAACT TCCAGAAGAT 
GTCGGCCATT GTTTTGTCAC TTGGGCTGAT AAGTTCCAGA TGTACGTCAG TTACTGTAAG 
AACAAACCCG ACTCCACGCA GCTCATCCTC GAACATGCCG GAGCATACTT CGATGAAATC 
CAGCAAAGAC ATCGCCTTGC TAACTCCATC TCCTCTTACC TGATCAAACC AGTGCAGAGA 
ATCACAAAGT ACCAGCTGCT TCTCAAGGAG CTGCTGACAT GCTGCGAGGA GGGAAAAGGT 
GAGATCAAGG ACGGACTGGA AGTGATGCTC AGCGTCCCTA AGAAAGCCAA CGACGCCATG 
CACCTGTCTA TGCTGGAGGG GTTTGATGGA AACATTGACT CCCAGGGAGA GCTGATACTT 
CAGGAATCCT TCCAGGTCTG GGATCCTAAG ACTCTGATCC GAAAGGGTCG GGACCGGCAC 
CTCTTCCTGT TTGAGATGTC TCTGGTGTTA AGCAAAGAAG TCAAAGATTC CAACGGGCGC 
AGCAAATACC TGTACAAGAG CAAGCTGTTT ACATCAGAAC TTGGAGTTAC GGAACATGTG 
GAGGGAGATC CCTGTAAGTT CGCCCTGTGG GTGGGCAGGA CCCCAACGTC TGACAACAAG 
ATCGTCCTCA AGGCATCCAG TATCGAGAAC AAACAGGACT GGATCAAACA CATCCGGGAG 
GTGATCCAGG AGCGGATTGT CCACCTGCGC GGCGCACTGA AGGAGCCCAT CCACATCCCC 
AAAACCACCA CAGCCAAACA CAAAGGCAGA CGGGATGGAG AGGAACTGGA CAGCCAGGGG 
GATGCCAGCA GCCAACCAGA TACCATCTCC ATTGCCTCGC GAACATCCCA GAACACAATG 
GACAGCGATA AGCTCTCCGG TGGCTGTGAG TTGACAGTGG TGATCCACGA CTTCGTGGCC 
AGTAATGGCA GCAGCGGCGA GCTGTCGGTG CGCCGAGGTC AGACTGTGGA AGTCCTGGAA 
CGGCTCCACG ACAAGCCCGA CTGGTGCCTG GTGCGGACCA CGGACCGTTC CCCGGCTCAG 
GAGGGCATCG TTCCCTGCTC CATGCTCTGC ATCGCTCACT CGAGGTCTTC CATGGAGATG 
GAAGGGCTCT TTAACCACAA AGACACATTA TCTGTATCCA GTAACGACGC CCTCCAACCA 
GGCTCCAGCC AAACCCTGGG TCCTCACAGC TCCCCCGGCC CCAAACGCCC AGGGAACACA 
CTGAGGAAGT GGCTGACCAG CCCCGTGCGG AGGCTCAGCT CTGGCAAGGC CGACGGCCAC 
GTGAAGAAAC TGGCCCACAA GCATAAGAAG AACCGCGAGG GCGCGAGGAA GAACATGGAT 
GCCATGCACG GTTCTCAGAA GGACTCTGAT GACAGCGCCG CTACGCCCCA GGATGAAACG 
CTAGAGGAAC GTATGCGTAA CGAAGGGCTG AGCAGCGGCA CCCTGTCCAA GTCTTCCTCT 
TCTGGCATGC AGAGCTGTGG CGAGGAGGAA GGAGAGGAGG GAGCCGACGC CGTCCCTCTA 
CCGCCCCCTA TGGCCATCCA GCAGCACAGC TTGCTGCAGC CTGACTCCCA GGACGACAAG 
TCCGGATCTC GTCTGTCCGC ACGTCCAAGC AGCTCAGAGA CGCCCAGCGC GGCCGAACTA 
GTCAGCGCCA TCGAGGAGCT GGTGAAGAGT AAGATGTCCC TGGAGGATCG TCCCAGCTCT 
CTGTCAGTAG AACAAGGTGA CAGCAGCTCT CCCTCCCTAA ATCCCTCTGA CAACTCCCTC 
CTCTCCTCCT CCTCACCCAT CGATGAGATG GACGAGCGCA AGGCCGACTT CCTCAAGAGA 
AGACATTACG TGCTACTGGA GTTGGTGGAG ACCGAGAGGG ACTATGTCAG AGATCTGGGA 
TCAGTGGTAG AGGGCTACAT GTGTAGGATG AAAGAAGAGG GTGTTCCTGA TGACATGAGG 
GGAAAAGACA AGATCGTCTT TGGAAACATC CATCAGATCT ACGACTGGCA CAAAGATTTT 
TTCCTGGGGG AGCTTGAGAA GTGTTTAGAG GATCCCGACA GGCTGGCACC TTTATTCATT 
AAACAGGAGC GGAGGCTGCA CATGTACATC GTGTACTGCC AGAACAAACC CAAGTCTGAG 
CACATCGTTT CAGAGTACAT CGACACTTAT TTTGAGGACC TAAAGCAGCG TTTGGGCCAC 
AGGCTGCAGA TCACTGATCT ATTAATCAAG CCAGTCCAGC GTATAATGAA GTACCAATTA 
CTGCTAAAAG ATCTTTTGAA AATGTCCAAG AAGGCTGGCT TGGATATGGC AGAGCTTGAG 
AAAGCTGTGG AGGTGATGTG CGTGGTTCCC AAACGCTGCA ACGACATGAT GAATGTTGGA 
CGCCTTCAGG GTTTTGAAGG TAAAATTGTG GCGCAGGGCA GATTACTGCT CCAAGATACC 
TTCATGGTGT CGGACCAGGA TGGGGGACTT CTGTCCAGGA TGAAGGAGAG AAGGGTTTTC 
TTATTTGAAC AGATAGTAAT CTTTAGCGAA CCTCTTGACA AAAAGAAGGG CTTCTCCACT 
CCAGGATACC TCTTTAAGAA CAGCATCAAG GTGAGCTGGT TGGGTTTAGA AGAACATTCA 
GAGGACCCTT GCAAGTTCAC GCTTACCTCC AGATCATCTA GTGGCAACTT GGAGAGATAC 
ACCCTCCATT CAATGAGTCC TGGGGTCAGT CAAGTCTGGG TTCACCAGAT TAGCCAGATA 
CTGGAGAACC AGCGCAACTT CCTCAATGCT TTGACATCCC CCATTGAATA CCAGAGAAAC 
CATGTGGGTA GTGGAGTGCC AGGTGGTCTT CCCAGCAGCA GCAGCAGCAG TACTGGAGGC 
CTACCAGGAG GCAGCAGTTC CATTGGCTCC TCTAGCATGG GAGTAGGAGG CGGCGGTGGC 
TCTGGGGGAC CTGGGAACAG CTCATCCTCC TCACTCTGCG GTCCCCGTTC CCGACCGTCC 
CGCATTCCAC AGCCATCGTC ACGACTCCCA CAACCCGTCC ACCACCACCA TCCCCCGGGC 
CCTGATGGGC CTGACAGATC AGCAGGTATG TGGCCGCCCT GCCACCCTCA GTCCCACCCA 
TCTAACTCTT ATTCAGACAG GGCCACAAAT GGAGAACCAT TAGGCTCAGA GTTCCCCAAG 
ATGAAAAGAT TGGAGCTTCA CGGAAATAAC AGCAACAACA GCAACAAGTC CTCAGACAGT 
TATGAAGACA CTCAGAAGCA CCCGGCAGCT GGTATACCGC AGATGGCTGT TGCGCCTCTG 
AATTTGACCC TAAAAAGCCC CCGAGTCGGG ACAGTTTCTC CTCTGATTTC ACCTCAGTCT 
CCTGCAGGAG GAAAAGAAGC ATTTATCCCT TCAAGTCAAG CTCATAAAGG CAACTTGTTC 
TGGGCTGTGG TCCCTCCTAT GCCTTCCTCT GTGTCGAGTC GGCCCAGTTC GCTCTCCTAC 
CCCAGCGATA GTGTTGGTGG AGGGGATTCT CTGGGAAGAG GCGGCCACTG TACGCCCTCT 
CACCATCGCC AGTCCACTCA CAGTAAGGAC ATAGACCGCA TGAGCACCTG CTCCTCCACC 
AGCGAGCAGT CCATACAGTC CATCCAGAGC AATGGGAGTG AAAACAGTAG CAGCAGCATT 
GTGTCCACCA TGTTGGTGAC CCAGGACTAT GTGGCAGTGA AGGAGGATGA GATCAGTGTG 
GTCCAGGGTG AGGTGGTCCA AATACTGGCC AGCAACCAAC AAAACATGTT CCTGGTGTAC 
CGGGCAGCCA ACGAGCACTC TCCTGCTGCT GAGGGCTGGA TCCCTGGCTA TGTATTAGGA 
CACACCTCAT CCTCTGTCAC ACCTGAGCTC CCTGAAGGAA CCATCAAAAA ATCACTATCA 
TGGCACACAG CACTCCGAAT TCGCCGTAAA TCTGAAAAAA GGGACAAGGA GGGCAGGAAG 
ATCGAGAACG GGTACCGCAA ATCTCAGGAC AGCCTGGCCA ACAAAGTCTC TGTTAAGCTT 
CTGAATCCCA ATTTCATTCC TGACGCACCT CCAGAATTCC TCATCCCTGT CACCGATGTA 
GCATGTGAGA GCGGCGACAC TGTGACACTC AGGTGCAAAA TCTGTAGCCG GCCTCGCGCC 
ACCGTTACCT GGCGAGGACC TGACAACGGC GTTCGGAGCA ACAACGGACG CTACAGCACC 
ACCTACAGTG AGACAGAAGA AGCGACTCTC CGTATTGTGA TGGTGTCCGT AGAGGACAGT 
GGCGTGTACA CGTGTGTCGC CACAAATGTT GCAGGCTCCG TCACGTCCTC CGCCAACCTC 
ACAGTCTCAG CTTCGCATGA TGGCAACAGT GAGATCTGGA AGAGCAGCTT TGAGTTCAAC 
TACACAGAGA TCACAGAACT AGGAAGGGGC CGTTTCTCAG TGACCAAACG GTGCGACCAG 
AGAGGGAGCA AGCGCACGGT TGCGGCCAAA TACGTTAGCA AGAAGCTGCT GCGGCGAGAG 
CAGGTGCTGC AGGAGATCAG ACTCCTGCAG ACTCTGGACC ATCCCAACCT GGTTAAGCTT 
CTGGACACCT ATGAAATGGC CAACAGCTAT GTTCTCGTGC TAGAAATGGC GGACCAGGGA 
CGTTTCTTGG ATTACGTAGT GAGCTGGGGC AACCTCACAG AGGAGAAGGT GGCACTGTAC 
CTCAGAGAAA TTCTTGAAGC TCTTCACTAC CTGCACGGCT GGAGGATAGC GCACCTCGAC 
CTCAAACCTG AGAACATTGT GGTGGAACAT GCATCCTCGC AGCCGGTGAT CAAGCTGACA 
GACTTTGGTG ATGCCGTGCA ACTGAATCCA GCCTCCTCGT ACATCCATCC TCTGCTGGGG 
AGCCCAGAGT TCTCTGCTCC TGAGCTGGTC CTCGGGCAGC CCTCCTCGCT GACGTCGGAC 
ATCTGGAGTT TAGGGGTGGT GACATACATT GTACTCAGTG GCGCTTCCCC CTTCCTGGAT 
GAGAGTTTGG AGGAGACGTG TCTGAACATC TGTCGCCTGG ACTTCAGCTT CCCGGAGGAC 
TACTTCCAGG GAGTGAGCCT GGCTGCCAAG GACTTTGTCT GCCTGCTGCT CCAGAGCGAG 
CCGGAGCGGC GGCCCACTGC GTTAGCCTGC CTGCAGGAGC AGTGGCTCCA GCCCCGAGGT 
GTGATCAATA CTGGATTTGT AGGCTCGAAA CATGCACACG CGTCGCCCAA CCATCACACT 
TTACATCTGG ACACATCCAG ACTCATTTCT TTCATTGAGA GGCGGAAACA CCAGAACGAC 
ATTCGGCCCA TAGACACGGT CAGGGCCTTC CTCCACAGCC GCCTGCTTAA CAACATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029695080.1
CDS	203..9562
Translation	MSAGEGLDEAAKDAADIAAFFKSGFHRNEDMKAIDVLPILKEKVAFLSGGRDRRGGPVLTFPARSNHDRIRPEDLRRLIAYLATIPSEEVARHGFTVIVDMRGSKWDSIKPLLKILQESFPSCIHVALIIKPDNFWQKQRTNFGSSKFEFETVMVSLEGLSKIVDPSQLTADFEGSLEYNHDDWIEVRLSFETFASDAARALARLEELQDTLSQRDLPRDLEGARRLMEEHAALKKRATKASVEELDTQGRRLLQRLQAQATGGGSVNESGYINRLGGASGDPNDHHHGCHTHADAHNLITKVSSLLEKLHGTRQNLQQLWHMRKLKLDQCFQLRLFEQDAEKMFDWIMHNKGLFLTSYTEIGGNHQHAVELQTQHNHFAMNCMNVYVNINRIMSVGNRLLESGHYASQQIQQISGQLEQEWKAFAAALDERSTLLEMSASFHQKADQYMGKVEPWCKACGEGELPSELQDLEDTIHHHQGLYEHITTAYSEVSQDGKSLLDKLQRPLTPGSADSLMASANYSKAVHHVLDIIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKVLLDMSVAFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQATVNVIKEGEDLIQQLRDSAISSNKTPHNSSMAHIESVLQQLDEAQGQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNEELSQQMSDFDTEDLTLAEQRLQHHADKALTMNNLTYDVIHQGQELLQYVTEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRRHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQKEHEQFQHAIEKTHQSALQVQQKAETLLQANHYDMDMIRDCAEKVADHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSESDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMLSHVKAPETQVKNILNELLQRENRVLHFWTMRKRRLDQCQQYVVFERSAKQALEWIHDTGEFYLSTHTSTGSSIHHTQELLKEHEDFQITAKQTKERVKLLIQLADGFCDKGHAHALEIKKWVSSVDKRYRDFSLRMDKYRTCLETALGISSDSNKASKELQLDIIPASAPGSEVKLRDAAHDLNEEKRKSARRKEFIMAELIQTEKAYVRDLRECLDTYLWEMTSGVEEIPAGIVNKEHIIFGNMQDLYEFHHNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVSYCKNKPDSTQLILEHAGAYFDEIQQRHRLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKKANDAMHLSMLEGFDGNIDSQGELILQESFQVWDPKTLIRKGRDRHLFLFEMSLVLSKEVKDSNGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERIVHLRGALKEPIHIPKTTTAKHKGRRDGEELDSQGDASSQPDTISIASRTSQNTMDSDKLSGGCELTVVIHDFVASNGSSGELSVRRGQTVEVLERLHDKPDWCLVRTTDRSPAQEGIVPCSMLCIAHSRSSMEMEGLFNHKDTLSVSSNDALQPGSSQTLGPHSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKNREGARKNMDAMHGSQKDSDDSAATPQDETLEERMRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKSGSRLSARPSSSETPSAAELVSAIEELVKSKMSLEDRPSSLSVEQGDSSSPSLNPSDNSLLSSSSPIDEMDERKADFLKRRHYVLLELVETERDYVRDLGSVVEGYMCRMKEEGVPDDMRGKDKIVFGNIHQIYDWHKDFFLGELEKCLEDPDRLAPLFIKQERRLHMYIVYCQNKPKSEHIVSEYIDTYFEDLKQRLGHRLQITDLLIKPVQRIMKYQLLLKDLLKMSKKAGLDMAELEKAVEVMCVVPKRCNDMMNVGRLQGFEGKIVAQGRLLLQDTFMVSDQDGGLLSRMKERRVFLFEQIVIFSEPLDKKKGFSTPGYLFKNSIKVSWLGLEEHSEDPCKFTLTSRSSSGNLERYTLHSMSPGVSQVWVHQISQILENQRNFLNALTSPIEYQRNHVGSGVPGGLPSSSSSSTGGLPGGSSSIGSSSMGVGGGGGSGGPGNSSSSSLCGPRSRPSRIPQPSSRLPQPVHHHHPPGPDGPDRSAGMWPPCHPQSHPSNSYSDRATNGEPLGSEFPKMKRLELHGNNSNNSNKSSDSYEDTQKHPAAGIPQMAVAPLNLTLKSPRVGTVSPLISPQSPAGGKEAFIPSSQAHKGNLFWAVVPPMPSSVSSRPSSLSYPSDSVGGGDSLGRGGHCTPSHHRQSTHSKDIDRMSTCSSTSEQSIQSIQSNGSENSSSSIVSTMLVTQDYVAVKEDEISVVQGEVVQILASNQQNMFLVYRAANEHSPAAEGWIPGYVLGHTSSSVTPELPEGTIKKSLSWHTALRIRRKSEKRDKEGRKIENGYRKSQDSLANKVSVKLLNPNFIPDAPPEFLIPVTDVACESGDTVTLRCKICSRPRATVTWRGPDNGVRSNNGRYSTTYSETEEATLRIVMVSVEDSGVYTCVATNVAGSVTSSANLTVSASHDGNSEIWKSSFEFNYTEITELGRGRFSVTKRCDQRGSKRTVAAKYVSKKLLRREQVLQEIRLLQTLDHPNLVKLLDTYEMANSYVLVLEMADQGRFLDYVVSWGNLTEEKVALYLREILEALHYLHGWRIAHLDLKPENIVVEHASSQPVIKLTDFGDAVQLNPASSYIHPLLGSPEFSAPELVLGQPSSLTSDIWSLGVVTYIVLSGASPFLDESLEETCLNICRLDFSFPEDYFQGVSLAAKDFVCLLLQSEPERRPTALACLQEQWLQPRGVINTGFVGSKHAHASPNHHTLHLDTSRLISFIERRKHQNDIRPIDTVRAFLHSRLLNNI

Target ORF information:

RefSeq Version	XM_029839220.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes triple functional domain protein-like (LOC101071578), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029839220.1

ORF Insert Sequence:

ATGAGCGCAG GAGAGGGATT AGATGAGGCT GCGAAGGACG CGGCAGACAT AGCGGCGTTT 
TTCAAATCCG GGTTTCACCG CAATGAGGAT ATGAAGGCCA TTGACGTACT TCCTATCCTC 
AAGGAGAAGG TCGCCTTTCT CTCAGGTGGC AGAGACAGAC GCGGAGGTCC CGTTCTCACA 
TTTCCTGCTC GGAGCAACCA TGACAGAATA CGACCTGAAG ACCTCCGCAG GCTCATAGCT 
TACCTGGCTA CTATTCCCAG CGAGGAAGTG GCGAGGCATG GCTTCACGGT CATTGTGGAC 
ATGCGTGGCT CTAAATGGGA CAGCATCAAG CCTTTGCTGA AGATCCTTCA GGAGTCGTTC 
CCATCTTGCA TCCACGTTGC CCTCATCATC AAGCCCGACA ACTTCTGGCA GAAGCAGAGG 
ACAAACTTCG GCAGCTCCAA ATTTGAATTC GAGACGGTGA TGGTCTCTTT AGAGGGTTTA 
TCCAAAATCG TGGACCCATC ACAGCTGACA GCTGACTTTG AGGGCAGCCT GGAATACAAC 
CATGATGACT GGATCGAGGT GCGGCTGTCC TTTGAGACGT TTGCCAGCGA TGCGGCACGT 
GCGCTGGCAC GCCTGGAGGA GCTCCAGGAC ACGTTGTCCC AGCGTGATCT CCCGCGGGAC 
CTGGAAGGAG CTCGGCGGCT CATGGAGGAA CACGCGGCGC TCAAGAAAAG GGCCACGAAA 
GCATCCGTGG AGGAGCTGGA CACGCAGGGG CGACGGCTGC TGCAGAGGCT GCAGGCACAG 
GCTACGGGAG GAGGCAGCGT TAATGAAAGC GGGTACATTA ACCGCCTGGG CGGAGCTAGC 
GGAGACCCCA ACGACCACCA TCACGGGTGC CACACGCACG CAGACGCACA CAACCTCATC 
ACCAAAGTGA GCAGCTTGTT GGAAAAGCTT CACGGGACTC GGCAAAATCT GCAGCAGCTG 
TGGCACATGA GGAAGCTGAA GCTGGACCAG TGTTTCCAGC TGCGTCTTTT TGAACAGGAC 
GCAGAGAAGA TGTTCGACTG GATCATGCAC AACAAGGGGC TTTTTCTCAC GAGTTACACG 
GAGATCGGGG GGAACCACCA GCACGCTGTC GAGCTGCAGA CCCAACACAA CCACTTTGCC 
ATGAACTGCA TGAATGTGTA CGTGAACATC AACCGGATCA TGTCAGTCGG GAACCGGCTC 
CTGGAGTCGG GCCATTACGC CTCGCAGCAG ATCCAGCAGA TCTCCGGGCA GCTGGAGCAG 
GAGTGGAAGG CCTTCGCTGC GGCGCTGGAT GAACGCAGCA CGCTGCTGGA GATGTCCGCC 
AGCTTCCACC AGAAAGCGGA CCAGTACATG GGGAAAGTGG AACCGTGGTG TAAAGCCTGT 
GGTGAAGGAG AGCTGCCCTC TGAACTCCAG GATCTGGAAG ACACAATCCA CCATCATCAG 
GGGCTTTATG AGCACATCAC CACTGCTTAC TCTGAGGTAA GCCAGGATGG GAAGTCACTC 
TTGGACAAGC TGCAGCGCCC TTTGACCCCA GGGAGCGCTG ATTCCTTGAT GGCCTCTGCC 
AACTACTCCA AGGCCGTGCA TCACGTCTTA GACATCATCC ACGAGGTGCT GCACCACCAG 
AGGCAGCTGG AAAACATCTG GCAGCATCGC AAAGTCCGCC TGCACCAACG GCTGCAACTG 
TGTGTTTTTC AGCAGGATGT GCAGCAGGTA CTGGACTGGA TAGAAAACCA CGGAGAAGCC 
TTCCTAAGCA AACACACTGG TGTTGGCAAG TCTCTGCACA GAGCCAGAGC TCTGCAGAAG 
AGGCATGAGG ATTTTGAAGA GGTTGCACAG AACACCTACA CTAATGCTGA CAAACTGCTG 
GAAGCCGCAG AGCAGCTGGC TCAGACCGGC GAATGTGATC CAGAGGAAAT TTACCAGGCT 
GCCCACCAGC TGGAAGATCG CATCCAGGAC TTCGTACGAC GTGTCGAGCA GAGAAAAGTC 
CTGTTGGACA TGTCTGTCGC CTTCCACACG CATGTCAAAG AGCTGTGGAC GTGGCTGGAG 
GAGTTGCAGA AGGAATTGCT AGATGACGTA TACGCAGAAT CCGTGGAGGC TGTTCAGGAC 
CTGATCAAAC GCTTCGGCCA GCAGCAGCAG ACCACCCTGC AGGCTACTGT CAATGTCATC 
AAAGAGGGAG AGGACCTTAT CCAACAGCTC AGAGACTCGG CTATCTCAAG CAACAAAACT 
CCCCATAACA GCTCCATGGC CCACATCGAG AGTGTGCTGC AGCAGCTGGA CGAGGCTCAG 
GGCCAGATGG AGGAACTGTT TCAGGAAAGG AAGATCAAAC TGGAGCTCTT TCTGCAGCTG 
CGCATCTTCG AGAGGGACGC CATCGATATC ATCTCTGACC TGGAGTCATG GAACGAGGAG 
CTCTCCCAGC AGATGAGTGA CTTCGACACC GAGGATCTGA CGCTGGCGGA GCAGAGGCTG 
CAGCACCACG CAGACAAGGC GCTCACCATG AACAACCTGA CCTACGACGT CATCCACCAG 
GGCCAAGAGC TGCTGCAGTA CGTGACCGAG GTCCAGGCGT CTGGTGTGGA GCTGCTGTGC 
GACAGAGATG TGGACATGGC CACACGGGTC CAGGACCTGC TGGAGTTCCT CCACGAGAAG 
CAGCAGGAGC TGGACCTGGC GGCGGAGCAG CACAGGAGAC ACTTGGAACA GTGTGTCCAA 
CTGAGACACC TCCAGGCAGA AGTCAAACAG GTGCTGGGAT GGATCCGGAA TGGCGAGTCC 
ATGCTGAACG CTGGTCTGAT CACAGCCAGC TCGTTACAGG AAGCTGAACA GCTGCAGAAG 
GAACATGAAC AATTTCAGCA TGCAATAGAG AAAACTCATC AGAGCGCGCT GCAGGTGCAG 
CAGAAGGCCG AGACCTTACT CCAGGCAAAC CACTATGACA TGGACATGAT CAGGGACTGT 
GCAGAAAAGG TGGCAGACCA CTGGCAGCAG CTCATGCTGA AAATGGAAGA CCGACTCAAG 
CTGGTCAACG CTTCTGTGGC CTTCTACAAA ACCTCCGAAC AGGTTTGCAG TGTGTTGGAA 
AGCCTGGAGC AGGAGTACAA GAGAGAAGAG GACTGGTGTG GTGGCGCTGA TAAACTAGGC 
CCCAACAGCG AGTCCGACCA CGTCACTCCC ATGATCAGCA AACATCTGGA GCAGAAAGAG 
GCCTTTCTCA AGGCGTGCAC GCTAGCCCGG CGGAATGCTG ACGTCTTCTT GAAGTACCTG 
CACAGAAACA GTGTCAATAT GCCCGGCATG CTGTCCCACG TCAAAGCTCC AGAGACTCAG 
GTTAAGAATA TCTTGAACGA GCTGCTCCAG AGAGAGAACC GAGTCCTTCA CTTCTGGACA 
ATGAGGAAGA GGAGGCTGGA CCAGTGTCAG CAATATGTCG TATTTGAACG CAGCGCCAAA 
CAGGCTCTGG AGTGGATCCA TGACACTGGG GAGTTTTACT TGTCCACACA CACATCCACT 
GGCTCCAGCA TTCATCACAC ACAAGAGCTG CTGAAGGAGC ATGAGGACTT CCAGATCACA 
GCAAAGCAAA CCAAAGAGCG AGTAAAGCTG TTGATCCAGC TGGCAGATGG GTTTTGTGAC 
AAGGGGCACG CCCATGCACT GGAAATAAAG AAATGGGTGT CTTCTGTGGA CAAGCGCTAC 
CGGGACTTCT CTCTCCGCAT GGACAAATAC CGTACCTGCC TGGAAACTGC GCTCGGCATA 
TCATCCGACT CCAACAAGGC TAGCAAAGAG CTCCAGCTGG ATATAATCCC AGCCAGCGCT 
CCGGGCTCAG AGGTCAAACT CCGGGATGCT GCCCATGACC TCAATGAGGA GAAGAGGAAG 
TCGGCACGGA GGAAGGAATT CATAATGGCG GAGCTGATTC AAACAGAAAA AGCCTACGTC 
AGGGACCTAA GGGAGTGTTT GGATACGTAC CTGTGGGAGA TGACCAGTGG TGTGGAGGAG 
ATTCCCGCCG GAATTGTCAA CAAAGAGCAC ATCATCTTTG GGAACATGCA GGACCTCTAC 
GAGTTCCACC ACAACATCTT CCTGAAGGAG TTGGAGAAGT ATGAGCAACT TCCAGAAGAT 
GTCGGCCATT GTTTTGTCAC TTGGGCTGAT AAGTTCCAGA TGTACGTCAG TTACTGTAAG 
AACAAACCCG ACTCCACGCA GCTCATCCTC GAACATGCCG GAGCATACTT CGATGAAATC 
CAGCAAAGAC ATCGCCTTGC TAACTCCATC TCCTCTTACC TGATCAAACC AGTGCAGAGA 
ATCACAAAGT ACCAGCTGCT TCTCAAGGAG CTGCTGACAT GCTGCGAGGA GGGAAAAGGT 
GAGATCAAGG ACGGACTGGA AGTGATGCTC AGCGTCCCTA AGAAAGCCAA CGACGCCATG 
CACCTGTCTA TGCTGGAGGG GTTTGATGGA AACATTGACT CCCAGGGAGA GCTGATACTT 
CAGGAATCCT TCCAGGTCTG GGATCCTAAG ACTCTGATCC GAAAGGGTCG GGACCGGCAC 
CTCTTCCTGT TTGAGATGTC TCTGGTGTTA AGCAAAGAAG TCAAAGATTC CAACGGGCGC 
AGCAAATACC TGTACAAGAG CAAGCTGTTT ACATCAGAAC TTGGAGTTAC GGAACATGTG 
GAGGGAGATC CCTGTAAGTT CGCCCTGTGG GTGGGCAGGA CCCCAACGTC TGACAACAAG 
ATCGTCCTCA AGGCATCCAG TATCGAGAAC AAACAGGACT GGATCAAACA CATCCGGGAG 
GTGATCCAGG AGCGGATTGT CCACCTGCGC GGCGCACTGA AGGAGCCCAT CCACATCCCC 
AAAACCACCA CAGCCAAACA CAAAGGCAGA CGGGATGGAG AGGAACTGGA CAGCCAGGGG 
GATGCCAGCA GCCAACCAGA TACCATCTCC ATTGCCTCGC GAACATCCCA GAACACAATG 
GACAGCGATA AGCTCTCCGG TGGCTGTGAG TTGACAGTGG TGATCCACGA CTTCGTGGCC 
AGTAATGGCA GCAGCGGCGA GCTGTCGGTG CGCCGAGGTC AGACTGTGGA AGTCCTGGAA 
CGGCTCCACG ACAAGCCCGA CTGGTGCCTG GTGCGGACCA CGGACCGTTC CCCGGCTCAG 
GAGGGCATCG TTCCCTGCTC CATGCTCTGC ATCGCTCACT CGAGGTCTTC CATGGAGATG 
GAAGGGCTCT TTAACCACAA AGACACATTA TCTGTATCCA GTAACGACGC CCTCCAACCA 
GGCTCCAGCC AAACCCTGGG TCCTCACAGC TCCCCCGGCC CCAAACGCCC AGGGAACACA 
CTGAGGAAGT GGCTGACCAG CCCCGTGCGG AGGCTCAGCT CTGGCAAGGC CGACGGCCAC 
GTGAAGAAAC TGGCCCACAA GCATAAGAAG AACCGCGAGG GCGCGAGGAA GAACATGGAT 
GCCATGCACG GTTCTCAGAA GGACTCTGAT GACAGCGCCG CTACGCCCCA GGATGAAACG 
CTAGAGGAAC GTATGCGTAA CGAAGGGCTG AGCAGCGGCA CCCTGTCCAA GTCTTCCTCT 
TCTGGCATGC AGAGCTGTGG CGAGGAGGAA GGAGAGGAGG GAGCCGACGC CGTCCCTCTA 
CCGCCCCCTA TGGCCATCCA GCAGCACAGC TTGCTGCAGC CTGACTCCCA GGACGACAAG 
TCCGGATCTC GTCTGTCCGC ACGTCCAAGC AGCTCAGAGA CGCCCAGCGC GGCCGAACTA 
GTCAGCGCCA TCGAGGAGCT GGTGAAGAGT AAGATGTCCC TGGAGGATCG TCCCAGCTCT 
CTGTCAGTAG AACAAGGTGA CAGCAGCTCT CCCTCCCTAA ATCCCTCTGA CAACTCCCTC 
CTCTCCTCCT CCTCACCCAT CGATGAGATG GACGAGCGCA AGGCCGACTT CCTCAAGAGA 
AGACATTACG TGCTACTGGA GTTGGTGGAG ACCGAGAGGG ACTATGTCAG AGATCTGGGA 
TCAGTGGTAG AGGGCTACAT GTGTAGGATG AAAGAAGAGG GTGTTCCTGA TGACATGAGG 
GGAAAAGACA AGATCGTCTT TGGAAACATC CATCAGATCT ACGACTGGCA CAAAGATTTT 
TTCCTGGGGG AGCTTGAGAA GTGTTTAGAG GATCCCGACA GGCTGGCACC TTTATTCATT 
AAACAGGAGC GGAGGCTGCA CATGTACATC GTGTACTGCC AGAACAAACC CAAGTCTGAG 
CACATCGTTT CAGAGTACAT CGACACTTAT TTTGAGGACC TAAAGCAGCG TTTGGGCCAC 
AGGCTGCAGA TCACTGATCT ATTAATCAAG CCAGTCCAGC GTATAATGAA GTACCAATTA 
CTGCTAAAAG ATCTTTTGAA AATGTCCAAG AAGGCTGGCT TGGATATGGC AGAGCTTGAG 
AAAGCTGTGG AGGTGATGTG CGTGGTTCCC AAACGCTGCA ACGACATGAT GAATGTTGGA 
CGCCTTCAGG GTTTTGAAGG TAAAATTGTG GCGCAGGGCA GATTACTGCT CCAAGATACC 
TTCATGGTGT CGGACCAGGA TGGGGGACTT CTGTCCAGGA TGAAGGAGAG AAGGGTTTTC 
TTATTTGAAC AGATAGTAAT CTTTAGCGAA CCTCTTGACA AAAAGAAGGG CTTCTCCACT 
CCAGGATACC TCTTTAAGAA CAGCATCAAG GTGAGCTGGT TGGGTTTAGA AGAACATTCA 
GAGGACCCTT GCAAGTTCAC GCTTACCTCC AGATCATCTA GTGGCAACTT GGAGAGATAC 
ACCCTCCATT CAATGAGTCC TGGGGTCAGT CAAGTCTGGG TTCACCAGAT TAGCCAGATA 
CTGGAGAACC AGCGCAACTT CCTCAATGCT TTGACATCCC CCATTGAATA CCAGAGAAAC 
CATGTGGGTA GTGGAGTGCC AGGTGGTCTT CCCAGCAGCA GCAGCAGCAG TACTGGAGGC 
CTACCAGGAG GCAGCAGTTC CATTGGCTCC TCTAGCATGG GAGTAGGAGG CGGCGGTGGC 
TCTGGGGGAC CTGGGAACAG CTCATCCTCC TCACTCTGCG GTCCCCGTTC CCGACCGTCC 
CGCATTCCAC AGCCATCGTC ACGACTCCCA CAACCCGTCC ACCACCACCA TCCCCCGGGC 
CCTGATGGGC CTGACAGATC AGCAGGTATG TGGCCGCCCT GCCACCCTCA GTCCCACCCA 
TCTAACTCTT ATTCAGACAG GGCCACAAAT GGAGAACCAT TAGGCTCAGA GTTCCCCAAG 
ATGAAAAGAT TGGAGCTTCA CGGAAATAAC AGCAACAACA GCAACAAGTC CTCAGACAGT 
TATGAAGACA CTCAGAAGCA CCCGGCAGCT GGTATACCGC AGATGGCTGT TGCGCCTCTG 
AATTTGACCC TAAAAAGCCC CCGAGTCGGG ACAGTTTCTC CTCTGATTTC ACCTCAGTCT 
CCTGCAGGAG GAAAAGAAGC ATTTATCCCT TCAAGTCAAG CTCATAAAGG CAACTTGTTC 
TGGGCTGTGG TCCCTCCTAT GCCTTCCTCT GTGTCGAGTC GGCCCAGTTC GCTCTCCTAC 
CCCAGCGATA GTGTTGGTGG AGGGGATTCT CTGGGAAGAG GCGGCCACTG TACGCCCTCT 
CACCATCGCC AGTCCACTCA CAGTAAGGAC ATAGACCGCA TGAGCACCTG CTCCTCCACC 
AGCGAGCAGT CCATACAGTC CATCCAGAGC AATGGGAGTG AAAACAGTAG CAGCAGCATT 
GTGTCCACCA TGTTGGTGAC CCAGGACTAT GTGGCAGTGA AGGAGGATGA GATCAGTGTG 
GTCCAGGGTG AGGTGGTCCA AATACTGGCC AGCAACCAAC AAAACATGTT CCTGGTGTAC 
CGGGCAGCCA ACGAGCACTC TCCTGCTGCT GAGGGCTGGA TCCCTGGCTA TGTATTAGGA 
CACACCTCAT CCTCTGTCAC ACCTGAGCTC CCTGAAGGAA CCATCAAAAA ATCACTATCA 
TGGCACACAG CACTCCGAAT TCGCCGTAAA TCTGAAAAAA GGGACAAGGA GGGCAGGAAG 
ATCGAGAACG GGTACCGCAA ATCTCAGGAC AGCCTGGCCA ACAAAGTCTC TGTTAAGCTT 
CTGAATCCCA ATTTCATTCC TGACGCACCT CCAGAATTCC TCATCCCTGT CACCGATGTA 
GCATGTGAGA GCGGCGACAC TGTGACACTC AGGTGCAAAA TCTGTAGCCG GCCTCGCGCC 
ACCGTTACCT GGCGAGGACC TGACAACGGC GTTCGGAGCA ACAACGGACG CTACAGCACC 
ACCTACAGTG AGACAGAAGA AGCGACTCTC CGTATTGTGA TGGTGTCCGT AGAGGACAGT 
GGCGTGTACA CGTGTGTCGC CACAAATGTT GCAGGCTCCG TCACGTCCTC CGCCAACCTC 
ACAGTCTCAG CTTCGCATGA TGGCAACAGT GAGATCTGGA AGAGCAGCTT TGAGTTCAAC 
TACACAGAGA TCACAGAACT AGGAAGGGGC CGTTTCTCAG TGACCAAACG GTGCGACCAG 
AGAGGGAGCA AGCGCACGGT TGCGGCCAAA TACGTTAGCA AGAAGCTGCT GCGGCGAGAG 
CAGGTGCTGC AGGAGATCAG ACTCCTGCAG ACTCTGGACC ATCCCAACCT GGTTAAGCTT 
CTGGACACCT ATGAAATGGC CAACAGCTAT GTTCTCGTGC TAGAAATGGC GGACCAGGGA 
CGTTTCTTGG ATTACGTAGT GAGCTGGGGC AACCTCACAG AGGAGAAGGT GGCACTGTAC 
CTCAGAGAAA TTCTTGAAGC TCTTCACTAC CTGCACGGCT GGAGGATAGC GCACCTCGAC 
CTCAAACCTG AGAACATTGT GGTGGAACAT GCATCCTCGC AGCCGGTGAT CAAGCTGACA 
GACTTTGGTG ATGCCGTGCA ACTGAATCCA GCCTCCTCGT ACATCCATCC TCTGCTGGGG 
AGCCCAGAGT TCTCTGCTCC TGAGCTGGTC CTCGGGCAGC CCTCCTCGCT GACGTCGGAC 
ATCTGGAGTT TAGGGGTGGT GACATACATT GTACTCAGTG GCGCTTCCCC CTTCCTGGAT 
GAGAGTTTGG AGGAGACGTG TCTGAACATC TGTCGCCTGG ACTTCAGCTT CCCGGAGGAC 
TACTTCCAGG GAGTGAGCCT GGCTGCCAAG GACTTTGTCT GCCTGCTGCT CCAGAGCGAG 
CCGGAGCGGC GGCCCACTGC GTTAGCCTGC CTGCAGGAGC AGTGGCTCCA GCCCCGAGGT 
GTGATCAATA CTGGATTTGT AGGCTCGAAA CATGCACACG CGTCGCCCAA CCATCACACT 
TTACATCTGG ACACATCCAG ACTCATTTCT TTCATTGAGA GGCGGAAACA CCAGAACGAC 
ATTCGGCCCA TAGACACGGT CAGGGCCTTC CTCCACAGCC GCCTGCTTAA CAACATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101071578 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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