Resources

List by Alphabet: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

rapid amplification of cDNA ends (RACE)

A PCR-based method to obtain cDNA clones of rare transcripts which begin with only a partial internal sequence. The 3'- and 5'-ends are analysed separately; a single (-)-strand is first constructed from the 3'-end of the transcript by use of reverse transcriptase and a poly(dT) primer. The (+)-3'-end is then generated in multiple copies by PCR using poly(dA) and the known internal partial sequence as primers. A single (-)-strand copy is constructed from the 5'-end using reverse transcriptase with a (-)-primer based on the known internal sequence. This 5'-(-)-transcript is tailed with poly(dA) and then used as a template in a PCR with the known internal sequence and poly(dA) as primers. Once the 3'- and 5'-ends are known, primers can be constructed to produce multiple copies of the gene by PCR. (see also RecA-assisted restriction cleavage by endonuclease (RACE cleavage))Frohman, M.A. (1990) in PCR Protocols: Rapid amplification of cDNA Ends (Innis, M.A., Gelfand, D.H., Sninsky, J.J. and White, T.J., eds.), pp. 28-38, Academic Press, San Diego

Related Biology Tools

  • GenSmart™ Codon Optimization

    GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.

    Learn More

  • DNA Construct Design Tool

    GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.

    Learn More

  • Codon Frequency Tables

    This online tool shows commonly used genetic codon frequency table in expression host organisms including Escherichia coli and other common host organisms.

    Learn More

Service and Products

Gene Synthesis

Offer gene synthesis of 100% sequence guarantee, free codon optimization, DNA synthesis in as fast as 4 days.

ORF cDNA Clones and Custom Clones

Largest commercial ORF clones database, 70,000 next-day shipping ORF clones.

Plasmid DNA Preparation

High quality plasmids supported by fully-automated platform, 100% full insert sequence accuracy guaranteed.

Express Cloning & Subcloning

$49 and 2 day turnaround, choose from over 150 IP FREE vectors.

Mutation and Library Services

Mutagenesis Portfolio offers several mutation and library services to meet various customers’ needs.

Oligo Synthesis

Bridge the gap between chemically synthesized oligos and bological applications.

If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: website@genscript.com

If your term is adopted, we will send 1,000 EzCoupon points to your GenScript account.